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candidate genes and quantitative trait loci affecting fruit ascorbic acid content in three tomato populations.fresh fruit and vegetables are a major source of ascorbic acid (vitamin c), an important antioxidant for the human diet and also for plants. ascorbic acid content in fruit exhibits a quantitative inheritance. quantitative trait loci (qtl) for ascorbic acid content have been mapped in three tomato populations derived from crosses between cultivated tomato varieties (solanum lycopersicum accessions) and three related wild species or subspecies. the first population consists of a set of introgressi ...200717277090
the construction of a solanum habrochaites lyc4 introgression line population and the identification of qtls for resistance to botrytis cinerea.tomato (solanum lycopersicum) is susceptible to grey mold (botrytis cinerea). partial resistance to this fungus has been identified in accessions of wild relatives of tomato such as solanum habrochaites lyc4. in a previous f(2) mapping study, three qtls conferring resistance to b. cinerea (rbcq1, rbcq2 and rbcq4a) were identified. as it was probable that this study had not identified all qtls involved in resistance we developed an introgression line (il) population (n = 30), each containing a s. ...200717273845
the inheritance of unilateral incompatibility in lycopersicon hirsutum. 196417248219
haploid selection for low temperature tolerance of tomato pollen.pollen grains were harvested from an interspecific f(1) hybrid between the cultivated tomato, lycopersicon esculentum mill., and its wild relative lycopersicon hirsutum humb. & bonpl., a low temperature tolerant accession originating from an altitude of 3200 m in the peruvian andes. the two species differ for electrophoretically-detectable loci that mark six (possibly seven) of the 12 tomato chromosomes. isozyme analysis of the bc(1) populations derived from controlled pollinations at normal and ...198217246078
comparative genetics of potential prezygotic and postzygotic isolating barriers in a lycopersicon species cross.i compare the genetic basis of quantitative traits that potentially contribute to pre- and postzygotic isolation between the plant species solanum lycopersicum (formerly lycopersicon esculentum) and solanum habrochaites (formerly lycopersicon hirsutum), using quantitative trait loci (qtl) mapping in a set of near-isogenic lines. putative prezygotic isolating traits include flower size, flower shape, stigma exertion, and inflorescence length, that can influence pollinator preferences and/or selfi ...200717229779
temporally extended gene expression of the adp-glc pyrophosphorylase large subunit (agpl1) leads to increased enzyme activity in developing tomato fruit.tomato plants (solanum lycopersicum) harboring the allele for the agpase large subunit (agpl1) derived from the wild species solanum habrochaites (agpl1 ( h )) are characterized by higher agpase activity and increased starch content in the immature fruit, as well as higher soluble solids in the mature fruit following the breakdown of the transient starch, as compared to fruits from plants harboring the cultivated tomato allele (agpl1 ( e )). comparisons of agpase subunit gene expression and prot ...200616770584
sucrose phosphate synthase, sucrose synthase, and invertase activities in developing fruit of lycopersicon esculentum mill. and the sucrose accumulating lycopersicon hirsutum humb. and bonpl.the green-fruited lycopersicon hirsutum humb. and bonpl. accumulated sucrose to concentrations of about 118 micromoles per gram fresh weight during the final stages of development. in comparison, lycopersicon esculentum mill. cultivars contained less than 15 micromoles per gram fresh weight of sucrose at the ripe stage. glucose and fructose levels remained relatively constant throughout development in l. hirsutum at 22 to 50 micromoles per gram fresh weight each. starch content was low even at e ...199116668028
sensitivity of altitudinal ecotypes of the wild tomato lycopersicon hirsutum to chilling injury.the transition temperature of the leaf polar lipids and the critical temperature for chill-induced inhibition of photosynthesis was determined for three altitudinal ecotypes of the wild tomato lycopersicon hirsutum. photosynthesis was measured as co(2)-dependent o(2) evolution at 25 degrees c after leaf slices were exposed to chilling temperatures for 2 hours at a moderate photon flux density of 450 micromoles per square meter per second. the transition temperature of the leaf polar lipids was d ...198916667203
physical properties of mitochondrial lipids from lycopersicon hirsutum.mitochondrial lipids from lycopersicon hirsutum undergo a broad thermal transition beginning well below 0 degrees c and ending at approximately 25 degrees c. differential thermal analysis of mitochondrial lipids isolated from ecotypes of l. hirsutum that differ in chilling sensitivity indicates that these lipid preparations have physically similar properties. this was confirmed by electron-spin-resonance experiments, although this technique failed to detect the broad transition detected by diffe ...198216662499
ripening behavior of wild tomato species.nine wild tomato species were surveyed for variability in ripening characteristics. external signs of ripening, age of fruit at ripening, and ethylene production patterns were compared. ethylene production was monitored using an ethylene-free air stream system and gas chromatography. based on these ripening characteristics, the fruits fell into three general categories: those that change color when they ripen, green-fruited species that abscise prior to ripening, and green-fruited species that r ...198116662121
temperature-induced leakage from chilling-sensitive and chilling-resistant plants.leakage rates were determined from leaf cells loaded with rubidium and [(3)h]leucine. there was a differential response between leucine and rubidium leakage depending upon the species used. the rate of leucine leakage shows a small decline below 5 c for two altitudinal variants of lycopersicon hirsutum humb. and bonpl., whereas lycopersicon esculentum l. showed a marked increase below 5 c. rubidium showed a marked increase in leakage rate below 10 c with the altitudinal variants, with only a sli ...198116661859
assay of chilling injury in wild and domestic tomatoes based on photosystem activity of the chilled leaves.tomato leaves were detached and stored at 0 c for various periods of time. chloroplasts were isolated from the leaves and their photoreductive activities were determined. comparisons were made between two altitudinal forms of the wild tomato lycopersicon hirsutum humb. and bonpl. (a tropical lowlands form and a highlands form adapted to growth at 3,100 meters), and two cultivars of the domestic tomato l. esculentum mill. in each case the capacity of the isolated chloroplasts to photoreduce ferri ...197916660815
temperature and photoperiod influence trichome density and sesquiterpene content of lycopersicon hirsutum f. hirsutum.resistance to colorado potato beetle in a clone of lycopersicon hirsutum f. hirsutum l. is attributed to the presence of the sesquiterpene zingiberene in the type vi leaf trichomes; however, both day/night temperature regimen and photoperiod affect zingiberene content and trichome density. in short days (sd), zingiberene content per trichome is more than 3-fold greater than in long days. in sd, trichome density per unit leaf surface is 2-fold greater at 25/20 degrees c (day/night) than at either ...199216653137
natural products: repellency and toxicity of wild tomato leaf extracts to the two-spotted spider mite, tetranychus urticae koch.the potential of using phytochemicals from leaves of wild tomato for controlling the two-spotted spider mite, tetranychus urticae koch, is explored in this study as a promising alternative to the use of synthetic pesticides. wild tomato accessions of lycopersicon hirsutum plants that are not consumed by humans were planted under greenhouse conditions for mass production of leaves. crude extracts from leaves of three accessions of l. hirsutum, six accessions of l. hirsutum f. glabratum, and one a ...200616393894
genetic transfer of a twospotted spider mite (acari: tetranychidae) repellent in tomato hybrids.lycopersicon hirsutum dunal is very resistant to arthropod herbivory, and research on causes of resistance has often implicated trichomes and their secretions. to better understand relationships among resistance, repellency, and 2,3-dihydrofarnesoic acid, a trichome-borne sesquiterpenoid spider mite repellent, two tomato, lycopersicon esculentum miller, varieties were interbred with a highly resistant, spider mite repellent accession (la1363) of l. hirsutum. backcross and f2 generations were pro ...200516334344
the cf-4 and cf-9 resistance genes against cladosporium fulvum are conserved in wild tomato species.the cf-4 and cf-9 genes originate from the wild tomato species lycopersicon hirsutum and l. pimpinellifolium and confer resistance to strains of the leaf mold fungus cladosporium fulvum that secrete the avr4 and avr9 elicitor proteins, respectively. homologs of cf-4 and cf-9 (hcr9s) are located in several clusters and evolve mainly through sequence exchange between homologs. to study the evolution of cf genes, we set out to identify functional hcr9s that mediate recognition of avr4 and avr9 (des ...200516167771
a major qtl introgressed from wild lycopersicon hirsutum confers chilling tolerance to cultivated tomato (lycopersicon esculentum).many plants of tropical or subtropical origin, such as tomato, suffer damage under chilling temperatures (under 10 degrees c but above 0 degrees c). an earlier study identified several quantitative trait loci (qtls) for shoot turgor maintenance (stm) under root chilling in an interspecific backcross population derived from crossing chilling-susceptible cultivated tomato (lycopersicon esculentum) and chilling-tolerant wild l. hirsutum. the qtl with the greatest phenotypic effect on stm was locate ...200516075210
occurrence of colombian datura virus in brugmansia hybrids, physalis peruviana l. and solanum muricatum ait. in hungary.colombian datura virus (cdv) has been found to infect angel trumpets (brugmansia spp.) frequently and cape gooseberry (physalis peruviana) and pepino (solanum muricatum) sporadically in hungary. a cdv brg/h isolate was characterized. it had flexuous thread-like virions of about 750 x 12 nm in size. host range and symptomathological studies revealed its great similarity to authentic cdv isolates. nicotiana tabacum cultivars and lines resistant to potato virus y (pvyn) either genically or transgen ...200516047739
metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones.medium-length methylketones (c7-c15) are highly effective in protecting plants from numerous pests. we used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato lycopersicon hirsutum f glabratum (accession pi126449). a comparison of gland est databases from accession pi126449 and a second l. hirsutum accession, la1777, whose glands do not contain methylketones, showed that the expression of genes for fatty ac ...200515772286
genetics of hybrid incompatibility between lycopersicon esculentum and l. hirsutum.we examined the genetics of hybrid incompatibility between two closely related diploid hermaphroditic plant species. using a set of near-isogenic lines (nils) representing 85% of the genome of the wild species lycopersicon hirsutum (solanum habrochaites) in the genetic background of the cultivated tomato l. esculentum (s. lycopersicum), we found that hybrid pollen and seed infertility are each based on 5-11 qtl that individually reduce hybrid fitness by 36-90%. seed infertility qtl act additivel ...200515466436
proteomic analysis of resistance mediated by rcm 2.0 and rcm 5.1, two loci controlling resistance to bacterial canker of tomato.two quantitative trait loci from lycopersicon hirsutum, rcm 2.0 and rcm 5.1, control resistance to clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. lines containing rcm 2.0 and rcm 5.1 and a susceptible control line were compared at 72 and 144 h postinoculation, using 2-dimensional gel electrophoresis to identify proteins regulated in response to c. michiganensis subsp. michiganensis infection. a total of 47 proteins were subjected to tandem mass sp ...200415384492
qtl analysis of quantitative resistance to phytophthora infestans (late blight) in tomato and comparisons with potato.quantitative trait loci (qtls) for resistance to phytophthora infestans (late blight) were mapped in tomato. reciprocal backcross populations derived from cultivated lycopersicon esculentum x wild lycopersicon hirsutum (bc-e, backcross to l. esculentum; bc-h, backcross to l. hirsutum) were phenotyped in three types of replicated disease assays (detached-leaflet, whole-plant, and field). linkage maps were constructed for each bc population with rflps. resistance qtls were identified on all 12 tom ...200415190365
mapping, genetic effects, and epistatic interaction of two bacterial canker resistance qtls from lycopersicon hirsutum.two quantitative trait loci (qtl) from lycopersicon hirsutum, rcm 2.0 and rcm 5.1, control resistance to clavibacter michiganensis subsp. michiganensis ( cmm). to precisely map both loci, we applied interval mapping techniques to 1,056 individuals in three populations exhibiting f(2) segregation. based on a 1-lod confidence interval, rcm 2.0 mapped to a 14.9-cm interval on chromosome 2 and accounted for 25.7-34.0% of the phenotypic variation in disease severity. rcm 5.1 mapped to a 4.3-cm interv ...200415067391
mutations in potato virus y genome-linked protein determine virulence toward recessive resistances in capsicum annuum and lycopersicon hirsutum.the recessive resistance genes pot-1 and pvr2 in lycopersicon hirsutum and capsicum annuum, respectively, control potato virus y (pvy) accumulation in the inoculated leaves. infectious cdna molecules from two pvy isolates differing in their virulence toward these resistances were obtained using two different strategies. chimeras constructed with these cdna clones showed that a single nucleotide change corresponding to an amino acid substitution (arg119his) in the central part of the viral protei ...200415000399
towards the introducing of resistance to powdery mildew from lycopersicon hirsutum into l. esculentum.genes of resistance to oidium lycopersicum from lycopersicon hirsutum la 1775 were introduced to l. esculentum. breeding procedures were based on a one-way programme up to the f2 generation and then four different methods were adopted to obtain f4 and bc4 populations. screening tests among those hybrid populations were performed in a greenhouse and showed segregation for resistance to powdery mildew due to different genetic backgrounds of the families derived from four breeding methods that chan ...200114564061
complex unilateral hybridization in lycopersicon hirsutum. 196113767338
zingiberene and curcumene in wild tomato.composition of ginger oil prepared from fresh ginger rhizomes, zingiber officinale roscoe (zingiberaceae) was determined by gas chromatography (gc) and gc-mass spectrometric techniques. the main sesquiterpene hydrocarbons identified were alpha-zingiberene (27-30%), alpha-curcumene (8-9%), beta-sesquiphellandrene (4.8%). and bisabolene (3.2%). the function of zingiberene and curcumene as insecticides, repellents, and insect feeding deterrents has been previously reported. other plant species havi ...200312856930
recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato ( lycopersicon spp.) and pepper ( capsicum spp.) genomes.resistance against both potato virus y (pvy) and tobacco etch virus (tev) was identified in the wild tomato relative lycopersicon hirsutum pi247087. analysis of the segregation ratio in f(2)/f(3) and bc(1) interspecific progenies indicated that a single recessive gene, or two very tightly linked recessive loci, are involved in resistance to both potyviruses. this locus was named pot-1. using amplified fragment length polymorphism markers and a set of l. hirsutum introgression lines, pot-1 was ma ...200212582910
identification of qtls for early blight ( alternaria solani) resistance in tomato using backcross populations of a lycopersicon esculentum x l. hirsutum cross.most commercial cultivars of tomato, lycopersicon esculentum mill., are susceptible to early blight (eb), a devastating fungal ( alternaria solani sorauer) disease of tomato in the northern and eastern parts of the u.s. and elsewhere in the world. the disease causes plant defoliation, which reduces yield and fruit quality, and contributes to significant crop loss. sources of resistance have been identified within related wild species of tomato. the purpose of this study was to identify and valid ...200212582599
sucrose uptake, invertase localization and gene expression in developing fruit of lycopersicon esculentum and the sucrose-accumulating lycopersicon hirsutum.by using immunolocalization and differential extraction methods we show that only apoplastic invertase, but not vacuolar invertase, was present in the mature, sucrose-accumulating l. hirsutum pericarp. in contrast, in the hexose-accumulating l. esculentum fruit, both the apoplastic and vacuolar invertase activities and protein content increase in the mature fruit. quantitative expression studies of the soluble invertase gene (tiv1) and the apoplastic invertase genes (lins) showed that only tiv1 ...200212010465
a molecular linkage map of tomato displaying chromosomal locations of resistance gene analogs based on a lycopersicon esculentum x lycopersicon hirsutum cross.a molecular linkage map of tomato was constructed based on a bc1 population (n = 145) of a cross between lycopersicon esculentum mill. line nc84173 (maternal and recurrent parent) and lycopersicon hirsutum humb. and bonpl. accession pi126445. nc84173 is an advanced breeding line that is resistant to several tomato diseases, not including early blight (eb) and late blight (lb). pi126445 is a self-incompatible accession that is resistant to many tomato diseases, including eb and lb. the map includ ...200211908656
production and quantification of methyl ketones in wild tomato accessions.production of methyl ketones as naturally occurring insecticides from wild tomato accessions is explored in this study. density of two glandular trichomes (type iv and type vi) on the leaves of six wild tomato accessions of lycopersicon hirsutum f. glabratum (mull); three accessions of l. hirsutum f. typicum (humb & bonpl.); two accessions of l. pennellii corr. (d'arcy); and density of type vi glandular trichomes of the commercial tomato lycopersicon esculentum cv. fabulous are reported. densiti ...200111757742
sesquiterpene carboxylic acids from a wild tomato species affect larval feeding behavior and survival of helicoverpa zea and spodoptera exigua (lepidoptera: noctuidae).the sesquiterpene carboxylic acids (sca), (+)-(e)-alpha-santalen-12-oic, (-)-(e)-endo-alpha-bergamoten-12-oic, and (+)-(e)-endo-beta-bergamoten-12-oic acid, are produced in glandular trichomes of lycopersicon hirsutum f. typicum humb. & bonpl. accession (la) 1777, which is highly resistant to a range of pests of cultivated tomatoes. l. esculentum mill. exposure of the larvae of two key tomato pests, tomato fruitworm [helicoverpa zea (boddie)] and beet armyworm [spodoptera exigua (hübner)], to th ...200111681691
evidence for involvement of phytochrome in tumor development on plants.the regulation of nonpathogenic tumorous growths on tomato plants by red and far-red radiation was studied using leaf discs floated on water and irradiated from beneath. it was found that red light (600-700 nanometers) was required for the induction of tumors on tomato (lycopersicon hirsutum humb. & bonpl. plant introduction la 1625), while both blue (400-500 nanometers) and green (500-600 nanometers) light had little effect on tumor development. detailed studies with red light demonstrated that ...198811537433
characterization and mapping of resistance to oidium lycopersicum in two lycopersicon hirsutum accessions: evidence for close linkage of two ol-genes on chromosome 6 of tomato.the cultivated tomato is susceptible to powdery mildew (oidium lycopersicum). several accessions of wild species are resistant. in this study we describe (i) the genetics and mapping of resistance to o. lycopersicum in g1.1290, one of the resistant accessions in lycopersicon hirsutum, (ii) fine mapping of ol-1 originated from l. hirsutum g1.1560, another resistant accession of l. hirsutum, and (iii) tests of allelism for resistance in g1.1290 and g1.1560. initially, it is demonstrated that the r ...200011240617
ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene pto.we have investigated the origin of the pto disease resistance (r) gene that was previously identified in the wild tomato species lycopersicon pimpinellifolium and isolated by map-based cloning. pto encodes a serine-threonine protein kinase that specifically recognizes strains of pseudomonas syringae pv. tomato (pst) that express the avirulence gene avrpto. we examined an accession of the distantly related wild species lycopersicon hirsutum var. glabratum that exhibits avrpto-specific resistance ...200111172075
development of a set of near isogenic and backcross recombinant inbred lines containing most of the lycopersicon hirsutum genome in a l. esculentum genetic background: a tool for gene mapping and gene discovery.a novel population consisted of a set of 99 near isogenic lines (nils) and backcross recombinant inbred lines (bcrils) derived from a cross between the cultivated tomato lycopersicon esculentum cv. e6206 and l. hirsutum accession la1777 is presented. most of the lines contain a single defined introgression from l. hirsutum in the l. esculentum genetic background and together, the lines provide a coverage of more than the 85% of the l. hirsutum genome. these lines represent a new tool to uncover ...200011081970
identification and ds-tagged isolation of a new gene at the cf-4 locus of tomato involved in disease resistance to cladosporium fulvum race 5.leaf mould disease in tomato is caused by the biotrophic fungus cladosporium fulvum. an ac/ds targeted transposon tagging strategy was used to isolate the gene conferring resistance to race 5 of c. fulvum, a strain expressing the avirulence gene avr4. an infection assay of 2-week-old seedlings yielded five susceptible mutants, of which two had a ds element integrated in the same gene at different positions. this gene, member of a gene family, showed high sequence homology to the c. fulvum resist ...19989670557
s-related protein can be recombined with self-compatibility in interspecific derivatives of lycopersicon.stylar proteins involved in the self-incompatible (si) response of lycopersicon hirsutum have been identified and mapped to the locus that controls si (s locus). l. esculentum, a self-compatible (sc) species of cultivated tomato, does not display these proteins. hybrids between sc l. esculentum and si l. hirsutum are self-sterile despite these individuals bearing pollen containing the s allele of l. esculentum. in progeny derived from backcrossing the hybrids to l. esculentum, there was a strong ...19958595049
Displaying items 101 - 139 of 139