Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the effect of condensed tannins from lotus pedunculatus and lotus corniculatus on the growth of proteolytic rumen bacteria in vitro and their possible mode of action. | five strains of proteolytic rumen bacteria were treated with condensed tannins (ct) purified from lotus pedunculatus and lotus corniculatus to investigate their effect on the growth of these bacteria in vitro. streptococcus bovis ncfb 2476, eubacterium sp. c124b, prevotella bryantii b14, butyrivibrio fibrisolvens h17c, and clostridium proteoclasticum b316t were tested against 200, 400, and 600 microg ct x ml(-1) extracted from l. pedunculatus and l. corniculatus. in the absence of ct, all bacter ... | 2001 | 11547882 |
| the bio operon on the acquired symbiosis island of mesorhizobium sp. strain r7a includes a novel gene involved in pimeloyl-coa synthesis. | the symbiosis island of mesorhizobium sp. strain r7a is a 500 kb chromosomal genetic element that upon transfer converts nonsymbiotic mesorhizobia to symbionts able to nodulate and fix nitrogen with lotus corniculatus. four genomic species of nonsymbiotic mesorhizobia have been isolated. all were auxotrophic for thiamin and biotin and three were auxotrophic for nicotinate, whereas derivatives of the strains containing the symbiosis island were prototrophic for all three vitamins. in this work, a ... | 2001 | 11320134 |
| the effect of birdsfoot trefoil (lotus corniculatus) and white clover (trifolium repens) in mixed pasture swards on incoming and established nematode infections in young lambs. | lotus corniculatus l., a leguminous plant containing condensed tannins, is now being evaluated in sweden as a component of mixed pastures. this is because of its high nutritive value, palatability, modest requirement for water, calcium and phosphorous and particularly because it has the ability to survive harsh weather conditions that typify northern scandinavia. this trial was undertaken to assess the possible parasitological benefits of using l. corniculatus when fed as a minor component in a ... | 2000 | 11234969 |
| symbiosis of astragalus cicer with its microsymbionts: partial nodc gene sequence, host plant specificity, and root nodule structure. | astragalus cicer (cicer milkvetch) nodule bacteria were investigated for host plant specificity and partial nodc gene sequences, whilst their native host was studied for the microscopic structure of root nodules. the strains under investigation formed nodules not only on the original host but also on astragalus glycyphyllos, astragalus sinicus, lotus corniculatus, and phaseolus vulgaris. the nodules induced on the cicer milkvetch were classified as indeterminate and characterized by apical, pers ... | 2000 | 11016697 |
| the implications of condensed tannins on the nutritive value of temperate forages fed to ruminants. | new methodology for measuring forage condensed tannin (ct) content is described and the effects of ct upon forage feeding and nutritive value for ruminant animals are reviewed. ct react with forage proteins in a ph-reversible manner, with reactivity determined by the concentration, structure and molecular mass of the ct. increasing concentrations of ct in lotus corniculatus and lotus pedunculatus reduce the rates of solubilization and degradation of fraction 1 leaf protein in the rumen and incre ... | 1999 | 10999013 |
| effect of condensed tannins extracted from four forages on the viability of the larvae of deer lungworms and gastrointestinal nematodes. | the inhibitory activity of condensed tannins extracted from four forage legume plants were evaluated by using a larval migration inhibition assay. the first (l1) and third (l3) stages of deer lungworm (dictyocaulus viviparus), and the third stage (l3) of deer gastrointestinal nematodes were incubated with tannins extracted from lotus pedunculatus, lotus corniculatus, sulla (hedysarum coronarium) and sainfoin (onobrychus viciifolia). the tannins extracted from all the forages had inhibitory activ ... | 2000 | 10955893 |
| effect of crop rotation and soil cover on alteration of the soil microflora generated by the culture of transgenic plants producing opines. | the culture of transgenic lotus corniculatus plants producing opines, which are bacterial growth substrates, leads to the selection of rhizospheric bacteria able to utilize these substrates. we have investigated the fate of the opine-utilizing community over time under different experimental conditions following elimination of selective pressure exerted by the transgenic plants. these plants were removed from the soil, which was either left unplanted or replanted with wild-type l. corniculatus o ... | 2000 | 10886651 |
| effect of forage legumes containing condensed tannins on lungworm (dictyocaulus sp.) and gastrointestinal parasitism in young red deer (cervus elaphus). | to investigate the effect of feeding forage legumes containing condensed tannins (ct) on internal parasitism, red deer calves were fed either lucerne (medicago sativa; 0.1 per cent ct), birdsfoot trefoil (lotus corniculatus; 1.9 per cent ct) or sulla (hedysarum coronarium; 3.5 per cent ct) and trickle-infected with deer-origin gastrointestinal nematode and lungworm (dictyocaulus sp.) larvae for 5 weeks, then slaughtered at 7 weeks. there was a significant negative linear relationship between die ... | 2000 | 10877967 |
| characterization of an atrazine-degrading pseudaminobacter sp. isolated from canadian and french agricultural soils. | atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. fourteen bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from soils obtained from two farms in canada and two farms in france. these strains were indistinguishable from each other based on repetitive extragenic palindromic pcr genomic fingerprinting performed with primers eric1r, eric2, and boxa1r. based on 16s rrna sequence analysis of one representativ ... | 2000 | 10877767 |
| the effect of condensed tannins from seven herbages on trichostrongylus colubriformis larval migration in vitro. | the effects of condensed tannins (ct) extracted from seven forages on the motility of the economically important nematode, trichostrongylus colubriformis (giles, 1892), were evaluated by using a larval migration inhibition (lmi) assay. the assay involved incubation of third stage (l3) exsheathed t. colubriformis larvae with ct extracted from lotus pedunculatus, lotus corniculatus, sulla (hedysarum coronarium), sainfoin (onobrychis viciifolia), dorycnium rectum, dorycnium pentaphyllum and dock (r ... | 2000 | 10833014 |
| lupine leghemoglobin i: expression in transgenic lotus and tobacco tissues. | the proximal parts of the promoters of the genes for symbiotic-type hemoglobins are generally conserved, but the promoter of the lbi gene of lupine (lulbi) shows some unusual structural features. it lacks typical organ-specific elements characteristic of all the leghemoglobin gene promoters described thus far. we have analysed its functional activity in transgenic lotus corniculatus. a fusion construct between the lbi promoter and the gus reporter gene was expressed mainly in the central zone of ... | 2000 | 10778735 |
| a new mesorhizobium loti hambi 1129 phage isolated from polish soil. | phage a1 isolated from the rhizosphere of lotus corniculatus was studied. it had a very narrow host range, as it was active only against mesorhizobium loti hambi 1129. phage a1 was classified as belonging to c bradley's group bacteriophages. the latent period of a1 was 120-130 min and a burst size 13-17 particles per cell. the nature of the phage receptor was examined. lipopolysaccharide from the phage-sensitive strain inactivated phage a1 in contrast to lps from the phage-resistant bacteria. pu ... | 2000 | 10706666 |
| a strong constitutive positive element is essential for the ammonium-regulated expression of a soybean gene encoding cytosolic glutamine synthetase. | in order to identify important promoter elements controlling the ammonium-regulated expression of the soybean gene gs15 encoding cytosolic glutamine synthetase, a series of 5' promoter deletions were fused to the gus reporter gene. to allow the detection of positive and negative regulatory elements, a series of 3' deletions were fused to a -90 camv 35s promoter fragment placed upstream of the gus gene. both types of construct were introduced into lotus corniculatus plants and soybean roots via a ... | 1999 | 10092182 |
| production, faecal egg counts and worm burdens of ewe lambs which grazed six contrasting forages. | lambs were grazed on monospecific swards of one of six forages, some containing condensed tannins (ct); sulla (hedysarum coronarium), lotus corniculatus and l. pedunculatus and some without ct (lucerne (medicago sativa), plantain (plantago lanceolata) and a ryegrass/white clover pasture (lolium perenne/trifolium repens)) to evaluate effects of forage type on gastrointestinal nematode burdens, faecal egg count as well as liveweight gain (lwg), wool growth and dag formation over a 42-day period. a ... | 1998 | 9877067 |
| lotus corniculatus nodulation specificity is changed by the presence of a soybean lectin gene | plant lectins have been implicated as playing an important role in mediating recognition and specificity in the rhizobium-legume nitrogen-fixing symbiosis. to test this hypothesis, we introduced the soybean lectin gene le1 either behind its own promoter or behind the cauliflower mosaic virus 35s promoter into lotus corniculatus, which is nodulated by r. loti. we found that nodulelike outgrowths developed on transgenic l. corniculatus plant roots in response to bradyrhizobium japonicum, which nod ... | 1998 | 9707526 |
| genetic manipulation of condensed tannins in higher plants. ii. analysis of birdsfoot trefoil plants harboring antisense dihydroflavonol reductase constructs | we have produced and analyzed transgenic birdsfoot trefoil (lotus corniculatus l.) plants harboring antisense dihydroflavonol reductase (as-dfr) sequences. in initial experiments the effect of introducing three different antisense antirrhinum majus l. dfr constructs into a single recipient genotype (s50) was assessed. there were no obvious effects on plant biomass, but levels of condensed tannins showed a statistical reduction in leaf, stem, and root tissues of some of the antisense lines. trans ... | 1998 | 9501146 |
| differential modification of flavonoid and isoflavonoid biosynthesis with an antisense chalcone synthase construct in transgenic lotus corniculatus. | three clonal genotypes of lotus corniculatus l. (bird's foot trefoil) were transformed with an antisense chalcone synthase (chs) gene construct made using a stress induced chs17 cdna from phaseolus vulgaris under the control of the constitutive camv 35s promoter and nos terminator via agrobacterium rhizogenes. after initial screening, ten antisense and five control co-transformation events from each recipient clonal genotype were analysed. after elicitation with glutathione, the level of tannin ... | 1997 | 9349273 |
| expression of an antirrhinum dihydroflavonol reductase gene results in changes in condensed tannin structure and accumulation in root cultures of lotus corniculatus (bird's foot trefoil). | condensed tannins (proanthocyanidins) are an important factor in the nutritive and dietary quality of many forage crops. we report here experiments aimed at altering the levels and monomer composition of condensed tannins (cts) in 'hairy root' cultures of lotus corniculatus (bird's foot trefoil) using genetic manipulation. an antirrhinum majus dihydroflavonol reductase (dfr) cdna was expressed in sense in l. corniculatus and ct levels in transgenic root cultures were analysed. two co-transformed ... | 1997 | 9349268 |
| overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic lotus corniculatus l. plants triggers changes in ammonium assimilation and plant development. | a soybean cytosolic glutamine synthetase gene (gs15) was fused with the constitutive 35s cauliflower mosaic virus (camv) promoter in order to direct overexpression in lotus corniculatus l. plants. following transformation with agrobacterium rhizogenes, eight independent lotus transformants were obtained which synthesized additional cytosolic glutamine synthetase (gs) in the shoots. to eliminate any interference caused by the t-dna from the ri plasmid, three primary transformants were crossed wit ... | 1997 | 9151446 |
| cell-specific expression of the promoters of two nonlegume hemoglobin genes in a transgenic legume, lotus corniculatus. | the promoters of the hemoglobin genes from the nitrogen-fixing tree parasponia andersonii and the related nonnitrogen-fixing trema tomentosa both confer beta-glucuronidase reporter gene expression to the central zone of the nodules of a transgenic legume, lotus corniculatus. beta-glucuronidase expression was high in the uninfected interstitial cells and parenchyma of the surrounding boundary layer and was low in the rhizobium-infected cells. this contrasts with the expression of both the p. ande ... | 1997 | 9008386 |
| controlling internal parasites in grazing ruminants without recourse to anthelmintics: approaches, experiences and prospects. | interest in ways of raising stock without using anthelmintics has been stimulated by the desire for intensive grazing systems to adopt more sustainable methods of internal parasite control and by potential premiums for "organic" produce. this requires grazing management systems which are practicable and achieve levels of parasite control sufficient to meet realistic production objectives. this paper will summarise 3 years experience of lamb and cattle production without a recourse to anthelminti ... | 1996 | 8923145 |
| a system for tissue-specific copper-controllable gene expression in transgenic plants: nodule-specific antisense of aspartate aminotransferase-p2. | a vector system, based on copper controllable gene expression, has been developed to give control over place as well as time of expression of an introduced gene. this system consists of two elements: (1) the yeast ace1 gene encoding a metallo-regulatory transcription factor, ace1, under control of either an organ-specific or a constitutive promoter; and (2) a gene of interest under control of a chimaeric promoter consisting of the 46 bp tata fragment of the camv 35s rna promoter linked to four r ... | 1996 | 8866892 |
| novel and complex chromosomal arrangement of rhizobium loti nodulation genes. | a mutational and structural analysis of rhizobium loti nodulation genes in strains nzp2037 and nzp2213 was carried out. unlike the case with other rhizobium strains examined to date, nodb was found on an operon separate from nodacij. sequence analysis of the nodacij and nodb operon regions confirm that r. loti common nod genes have a gene organization different from that of other rhizobium spp. at least 4 copies of nodd-like sequences were identified in r. loti. the complete nucleotide sequence ... | 1996 | 8850088 |
| differential expression of the sesbania rostrata leghemoglobin glb3 gene promoter in transgenic legume and non-legume plants. | the involvement of the sesbania rostrata glb3 gene promoter nice (nodule-infected cell expression) element in root-enhanced expression of 5'-srglb3-uida-3'nos chimeric gene was investigated in transgenic nicotiana tabacum plants. the full-length wild-type srglb3 promoter directed root meristem-enhanced expression in transgenic tobacco plants. the expression pattern of nine selected srglb3 promoter mutations in the nice element was examined in transgenic tobacco plants and compared with the patte ... | 1996 | 8806425 |
| four unnamed species of nonsymbiotic rhizobia isolated from the rhizosphere of lotus corniculatus. | previously, we found that genetically diverse rhizobia nodulating lotus corniculatus at a field site devoid of naturalized rhizobia had symbiotic dna regions identical to those of icmp3153, the inoculant strain used at the site (j. t. sullivan, h. n. patrick, w. l. lowther, d. b. scott, and c. w. ronson, proc. natl. acad. sci. usa 92:8985-8989, 1995). in this study, we characterized seven nonsymbiotic rhizobial isolates from the rhizosphere of l. corniculatus. these included two from plants at t ... | 1996 | 8702274 |
| agrobacterium rhizogenes as a vector for transforming higher plants. application in lotus corniculatus transformation. | 1995 | 8563829 | |
| minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from glycine max l. have different properties. | the characteristics of the soybean leghemoglobin lba gene promoter were analyzed and important promoter elements from the lba and lbc3 promoters were compared using transgenic lotus corniculatus plants. a 5' deletion analysis of the lba promoter delimited two cis-acting elements controlling expression: a distal positive element (-1254, -884) required for expression and a proximal element (-285, -60) essential for full-level activity. in contrast to the corresponding region of the lbc3 promoter, ... | 1993 | 8400139 |
| interaction of a rhizobial dna-binding protein with the promoter region of a plant leghemoglobin gene. | a nucleotide sequence was identified approximately 650 bp upstream of the sesbania rostrata leghemoglobin gene srglb3 start codon, which interacts specifically with a proteinaceous dna-binding factor found in nodule extracts but not in extracts from leaves or roots. the binding site for this factor was delimited using footprinting techniques. the dna-binding activity of this factor was found to be heat stable, dependent on divalent cations, and derived from the (infecting) azorhizobium caulinoda ... | 1993 | 8278541 |
| the quest for a herbal contraceptive. | an oral herbal contraceptive would allow couples control their fertility without consulting a health worker, which in turn would likely markedly increase the number of couples practicing family planning. other advantages of such a contraceptive would include the familiarity rural people have with herbal medicines, the fewer side effects associated with herbal preparations, their ready availability from local sources, and protection of privacy. there are many references to plants in india with ... | 1993 | 8241931 |
| site-specific mutagenesis of the nodule-infected cell expression (nice) element and the at-rich element atre-bs2* of the sesbania rostrata leghemoglobin glb3 promoter. | sesbania rostrata leghemoglobin glb3 (srglb3) promoter sequences responsible for expression in infected cells of transgenic lotus corniculatus nodules were delimited to a 78-bp dral-hinfl fragment. this region, which is located between coordinates -194 to -116 relative to the start codon of the srglb3 gene, was named the nodule-infected cell expression (nice) element. insertion of the nice element into the truncated nopaline synthase promoter was found to confer a nodule-specific expression patt ... | 1994 | 8180496 |
| effect of condensed tannins from birdsfoot trefoil on endoglucanase activity and the digestion of cellulose filter paper by ruminal fungi. | the ruminal fungi neocallimastix frontalis re1, neocallimastix patriciarum 27, piromyces communis 22, and orpinomyces joyonii 19-2 were examined for their ability to digest filter paper in the presence of condensed tannins from birdsfoot trefoil (lotus corniculatus l.). for all four fungi, inhibition of endoglucanases was evident at 100 micrograms condensed tannins.ml-1 with nearly complete inhibition at 300 micrograms condensed tannins.ml-1. at 100 and 200 micrograms condensed tannins.ml-1, the ... | 1994 | 8039053 |
| a protein binding at-rich sequence in the soybean leghemoglobin c3 promoter is a general cis element that requires proximal dna elements to stimulate transcription. | a nodule nuclear factor, nat2, interacts with two at-rich binding sites (nat2 bs1 and nat2 bs2) in the soybean leghemoglobin (lb) c3 promoter. in transgenic lotus corniculatus nodules, an oligonucleotide containing nat2 bs1 activated an inactive -159 lbc3 promoter when placed immediately upstream of the promoter. the activation was independent of the orientation of nat2 bs1 but was dependent on its position in the promoter. the abilities of different mutated binding sites to activate expression ... | 1994 | 8038605 |
| proanthocyanidins inhibit hydrolysis of leaf proteins by rumen microflora in vitro. | proanthocyanidins (condensed tannins; pa) purified from the leaves of forage legumes trifolium arvense, lotus pedunculatus, lotus corniculatus, dorycnium rectum, coronilla varia, onobrychis viciifolia, or hedysarum coronarium, were added to soluble lucerne (medicago sativa) leaf protein and incubated with strained rumen fluid in vitro. fractions were collected and frozen immediately. denatured proteins were fractionated by sodium dodecylsulphate-polyacrylamide gel electrophoresis (sds-page), sta ... | 1994 | 8031741 |
| identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic lotus corniculatus. | a dna fragment containing sequences hybridizing to the 5' region of gs15, a gene encoding soybean cytosolic glutamine synthetase, was isolated from a soybean genomic library. mapping and partial sequence analysis of the genomic clone revealed that it encodes a cytosolic gs gene, gs21, which is different from gs15. in parallel, a number of cdna clones encoding cytosolic gs were isolated using the coding region of pgs20 as a probe (pgs20 is a cdna clone which corresponds to a transcript of the gs1 ... | 1995 | 7865779 |
| the expression of a chimeric phaseolus vulgaris nodulin 30-gus gene is restricted to the rhizobially infected cells in transgenic lotus corniculatus nodules. | in phaseolus vulgaris there is a nodulin family, npv30, of ca. 30 kda, as detected in an in vitro translation assay [2]. we isolated a gene (npv30-1) for one of the members of this family. the nucleotide sequence of the promoter of npv30-1 contains nodule-specific motifs common to other late nodulin genes. the promoter was fused to the gus reporter gene; this chimeric fusion was introduced into lotus corniculatus via agrobacterium rhizogenes transformation. gus activity was only detected in the ... | 1994 | 7858234 |
| the effect of condensed tannins in lotus corniculatus on plasma metabolism of methionine, cystine and inorganic sulphate by sheep. | fresh lotus corniculatus containing 27 g extractable condensed tannin (ct)/kg dry matter (dm) and 8 g bound ct/kg dm was fed at hourly intervals to sheep held in metabolism cages to study the effects of ct on nutrient digestion and on metabolism of methionine, cystine and inorganic sulphate in plasma. polyethylene glycol (peg) was continuously infused into the rumen of half the sheep to remove the effects of ct. principal measurements in the two groups were plasma irreversible loss (irl) rate an ... | 1994 | 7827012 |
| symbiotic and nonsymbiotic hemoglobin genes of casuarina glauca. | casuarina glauca has a gene encoding hemoglobin (cashb-nonsym). this gene is expressed in a number of plant tissues. casuarina also has a second family of hemoglobin genes (cashb-sym) expressed at a high level in the nodules that casuarina forms in a nitrogen-fixing symbiosis with the actinomycete frankia. both the nonsymbiotic and symbiotic genes retained their specific patterns of expression when introduced into the legume lotus corniculatus. we interpret this finding to mean that the controls ... | 1995 | 7756831 |
| soybean nodulin-26 gene encoding a channel protein is expressed only in the infected cells of nodules and is regulated differently in roots of homologous and heterologous plants. | nodulin-26 (n-26) is a major peribacteroid membrane protein in soybean root nodules. the gene encoding this protein is a member of an ancient gene family conserved from bacteria to humans. n-26 is specifically expressed in root nodules, while its homolog, soybean putative channel protein, is expressed in vegetative parts of the plant, with its highest level in the root elongation zone. analysis of the soybean n-26 gene showed that its four introns mark the boundaries between transmembrane domain ... | 1993 | 7689881 |
| pasture management strategies for reducing the risk of legume bloat in cattle. | results from two decades (1973-1993) of bloat research at kamloops are reviewed. the trials were conducted with groups of ruminally fistulated cattle either grazing or fed daily fresh-cut alfalfa (medicago sativa) herbage. studies were conducted during the growing season (may to september) and in the fall (october and november). the alfalfa was usually in the vegetative to early bloom stages of growth. visual assessments of bloat severity on a scale of 1 to 5 were made .5 to 2 h after feeding st ... | 1995 | 7665381 |
| nodulating strains of rhizobium loti arise through chromosomal symbiotic gene transfer in the environment. | rhizobia were isolated from nodules off a stand of lotus corniculatus established with a single inoculant strain, icmp3153, 7 years earlier in an area devoid of naturalized rhizobium loti. the isolates showed diversity in growth rate, spe i fingerprint of genomic dna, and hybridization pattern to genomic dna probes. the 19% of isolates that grew at the same rate as strain icmp3153 were the only isolates that had the same fingerprint as strain icmp3153. sequencing of part of the 16s rrna gene of ... | 1995 | 7568057 |
| in vitro digestion of bloat-safe and bloat-causing legumes by rumen microorganisms: gas and foam production. | leaves of three bloat-safe legumes -- birdsfoot trefoil (lotus corniculatus l.), sainfoin (onobrychis viciaefolia scop.), and cicer milkvetch (astralagus cicer l.) -- and of three bloat-causing legumes -- alfalfa (medicago sativa l.), red clover (trifolium pratense l.), and white clover (trifolium repens l.) -- were incubated with strained rumen fluid or with mixed rumen fluid and solids. gas released was measured during the early period (0 to 22 h) of this in vitro digestion. gas volume was gre ... | 1980 | 7419778 |
| the effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on lotus corniculatus l. | sheep were used to evaluate the nutritional consequences of a low condensed-tannin concentration (22 g/kg dry matter (dm)) in lotus (lotus corniculatus l.) (control group) compared with lotus given to sheep receiving intraruminal polyethylene glycol (peg) infusion (peg group). peg selectively binds to tannins and prevents tannins from binding proteins. dm intakes (1430 (se 28) g/d) and digestibility of energy (663 (se 4.5) kj/mj intake) were similar for both groups but the apparent digestion of ... | 1987 | 3801377 |
| root nodule specific gene regulation: analysis of the soybean nodulin n23 gene promoter in heterologous symbiotic systems. | the nodulin n23 gene promoter was analysed in transgenic plants using the chloramphenicol acetyltransferase (cat) coding sequence as a reporter. a 5' flanking region of less than 1 kb was sufficient for the organ-specific expression of a chimeric n23-cat-3'lbc3 gene in root nodules formed on lotus corniculatus and trifolium repens after infection by their respective rhizobium symbionts. expression was regulated at the level of rna in both species of transgenic plants. promoter deletion analysis ... | 1988 | 3340542 |
| two glutamine synthetase genes from phaseolus vulgaris l. display contrasting developmental and spatial patterns of expression in transgenic lotus corniculatus plants. | the gln-gamma gene, which specifies the gamma subunit of glutamine synthetase in phaseolus vulgaris l., has been isolated and the regulatory properties of its promoter region analyzed in transgenic lotus corniculatus plants. a 2-kilobase fragment from the 5'-flanking region of gln-gamma conferred a strongly nodule-enhanced pattern of expression on the beta-glucuronidase reporter gene. parallel studies on the promoter of another glutamine synthetase gene (gln-beta) showed that a 1.7-kilobase frag ... | 2008 | 2577727 |
| interdependence and nodule specificity of cis-acting regulatory elements in the soybean leghemoglobin lbc3 and n23 gene promoters. | the qualitative and quantitative contributions of four separate cis-acting dna elements controlling the root nodule-specific soybean leghemoglobin lbc3 gene were analyzed in transgenic lotus corniculatus plants. expression from internal deletions in the 5' region between positions -49 and -1956 was monitored from a cat reporter gene. the strong positive element (spe; -1090, -947) responsible for high-level expression was demonstrated to be an organ-specific element by deleting proximal nodule-sp ... | 1990 | 2338938 |
| nonlegume hemoglobin genes retain organ-specific expression in heterologous transgenic plants. | hemoglobin genes from the nitrogen-fixing nonlegume parasponia andersonii and the related non-nitrogen-fixing nonlegume trema tomentosa have been isolated [landsmann et al. (1986). nature 324, 166-168; bogusz et al. (1988). nature 331, 178-180]. the promoters of these genes have been linked to a beta-glucuronidase reporter gene and introduced into both the nonlegume nicotiana tabacum and the legume lotus corniculatus. both promoters directed root-specific expression in transgenic tobacco. when t ... | 1990 | 2136637 |
| functional analysis of the sesbania rostrata leghemoglobin glb3 gene 5'-upstream region in transgenic lotus corniculatus and nicotiana tabacum plants. | expression of the sesbania rostrata leghemoglobin glb3 gene was analyzed in transgenic lotus corniculatus and tobacco plants harboring chimeric glb3-uida (gus) gene fusions to identify cis-acting elements involved in nodule-specific gene expression and general transcriptional control. a 1.9-kilobase fragment of the glb3 5'-upstream region was found to direct a high level of nodule-specific beta-glucuronidase (gus) activity in l. corniculatus, restricted to the rhizobium-infected cells of the nod ... | 1990 | 2136628 |
| a two-component nodule-specific enhancer in the soybean n23 gene promoter. | the two positive cis elements in the soybean nodulin n23 gene promoter were investigated in transgenic lotus corniculatus plants and shown to constitute a two-component nodule-specific enhancer. equal quantitative contributions from the two components were suggested by the similar expression level of chimeric n23-chloramphenicol acetyltransferase genes after deletion of either the distal positive element (pe-a, -320 to -298) or the proximal positive element (pe-b, -257 to -165). a combined effec ... | 1991 | 1820821 |
| ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic lotus corniculatus. | a full-length cdna clone encoding cytosolic glutamine synthetase (gs), expressed in roots and root nodules of soybean, was isolated by direct complementation of an escherichia coli gln a- mutant. this sequence is induced in roots by the availability of ammonia. a 3.5-kilobase promoter fragment of a genomic clone (lambda gs15) corresponding to this cdna was isolated and fused with a reporter [beta-glucuronidase (gus)] gene. the gs-gus fusion was introduced into a legume (lotus corniculatus) and a ... | 1991 | 1688099 |
| forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme. | glutamine synthetase (gs; ec 6.3.1.2) is present in different subcellular compartments in plants. it is located in the cytoplasm in root and root nodules while generally present in the chloroplasts in leaves. the expression of gs gene(s) is enhanced in root nodules and in soybean roots treated with ammonia. we have isolated four genes encoding subunits of cytosolic gs from soybean (glycine max l. cv. prize). promoter analysis of one of these genes (gs15) showed that it is expressed in a root-spe ... | 1992 | 1356501 |
| functional analysis of the promoter region of a nodule-enhanced glutamine synthetase gene from phaseolus vulgaris l. | the 5'-flanking region of gln-gamma, the nodule-enhanced glutamine synthetase gene from phaseolus vulgaris l., has been analysed for cis-regulatory elements using a series of 5' deletions and hybrid gln-gamma:: camv 35s promoters. the promoters were fused to the uida reporter gene and their activities tested in two heterologous expression systems. in the first system, the chimaeric genes were transferred to lotus corniculatus l. using agrobacterium rhizogenes and their expression was studied in ... | 1992 | 1353692 |