Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| paenibacillus wooponensis sp. nov., isolated from wetland freshwater. | a rod-shaped enodospore-forming gram-positive bacterium, strain wpcb018t, was isolated from a fresh water sample collected from woopo wetland, korea. the isolate was identified as a member of the genus paenibacillus on the basis of phenotypic characteristics and phylogenetic inference based on 16s rrna gene sequence. the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and unknown aminophospholipids. the diamino acid found in the cell wall peptidoglycan is meso-diaminopime ... | 2011 | 21216920 |
| characterization of a paenibacillus woosongensis beta-xylosidase/alpha-arabinofuranosidase produced by recombinant escherichia coli. | a gene encoding the beta-xylosidase/alpha-arabinofuranosidase (xylc) of paenibacillus woosongensis was cloned into escherichia coli. this xylc gene consisted of 1,425 nucleotides, encoding a polypeptide of 474 amino acid residues. the deduced amino acid sequence exhibited an 80% similarity with those of both clostridium stercorarium beta-xylosidase/alpha-n-arabinosidase and bacillus cellulosilyticus alpha-arabinofuranosidase, belonging to the glycosyl hydrolase family 43. the structural gene was ... | 2010 | 21193828 |
| assessment of root-associated paenibacillus polymyxa groups on growth promotion and induced systemic resistance in pepper. | twenty-nine p. polymyxa strains isolated from rhizospheres of various crops were clustered into five genotypic groups on the basis of box-pcr analysis. the characteristics of several plant growth-promoting factors among the isolates revealed the distinct attributes in each allocated group. under gnotobiotic conditions, inoculation of pepper roots with p. polymyxa isolates significantly increased the biomass in 17 of total 29 treated plants with untreated plants. experiments on induced systemic r ... | 2010 | 21193814 |
| production of a newly isolated paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater. | a phyllosphere bacterial strain ebl-06 was isolated from wheat leaves. the morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. a phylogenetic tree was constructed by comparing with the published 16s rdna sequences of the relevant bacteria. the results showed that the isolate ebl-06 was a strain of paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad ... | 2010 | 21174972 |
| genome sequence of the pattern forming paenibacillus vortex bacterium reveals potential for thriving in complex environments. | the pattern-forming bacterium paenibacillus vortex is notable for its advanced social behavior, which is reflected in development of colonies with highly intricate architectures. prior to this study, only two other paenibacillus species (paenibacillus sp. jdr-2 and paenibacillus larvae) have been sequenced. however, no genomic data is available on the paenibacillus species with pattern-forming and complex social motility. here we report the de novo genome sequence of this gram-positive, soil-dwe ... | 2010 | 21167037 |
| cell surface display of cellulase activity-free xylanase enzyme on saccharomyces cerevisiae eby100. | cellulase-free xylanase has potential for its application in the selective removal of hemicellulose from kraft pulp to give good strength to paper. in this study, a gene (xyn) encoding cellulase activity-free xylanase enzyme (xyn) was isolated from paenibacillus polymyxa ppl-3. the xyn gene encoded a protein of 221 amino acids, and the purified xyn was about 22.5 kda measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. moreover, the cellulase activity-free xylanase enzyme (xyn) ... | 2010 | 21161608 |
| effects of plant growth promoting rhizobacteria (pgpr) on rooting and root growth of kiwifruit (actinidia deliciosa) stem cuttings. | the effects of plant growth promoting rhizobacteria (pgpr) on the rooting and root growth of semi-hardwood and hardwood kiwifruit stem cuttings were investigated. the pgpr used were bacillus rc23, paenibacillus polymyxa rc05, bacillus subtilis osu142, bacillus rc03, comamonas acidovorans rc41, bacillus megaterium rc01 and bacillus simplex rc19. all the bacteria showed indole-3-acetic acid (iaa) producing capacity. among the pgpr used, the highest rooting ratios were obtained at 47.50% for semi-h ... | 2010 | 21157636 |
| [genotypic analysis and plant growth-promoting ability of four plant growth-promoting bacteria from mangrove]. | we identified four strains of plant growth-promoting bacteria (pgpb) and their plant growth-promoting ability. | 2010 | 21141471 |
| paenibacillus polymyxa jb05-01-1 and its perspectives for food conservation and medical applications. | the aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a livestock feed prebiotic supplement. a novel strain, termed paenibacillus polymyxa jb05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical properties as well as its 16s rrna gene sequence. this strain was able to inhibit growth of gram-negative bacteria including escherichia coli rr1, pseudomonas fluores ... | 2010 | 21132276 |
| study of a novel glycoconjugate, thiopeptidoglycan, and a novel polysaccharide lyase, thiopeptidoglycan lyase. | a typical filamentous bacterium, sphaerotilus natans, secretes a thiolic glycoconjugate which is assembled into a microtube, so called sheath. the glycoconjugate is known to consist of a pentasaccharide-dipeptide repeating unit, but its chemical structure has not been completely elucidated. in order to determine its chemical structure, the sheath was broken down by performic acid oxidation. the released sulfonated derivative was water soluble which was suitable for detailed nmr analysis. the dat ... | 2010 | 21095202 |
| harvest of scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures. | the optimal flocculating conditions for harvesting high-density cultures of scenedesmus sp. were investigated using inorganic coagulants and the bioflocculant produced by paenibacillus polymyxa am49. the flocculated medium as nutrients for subsequent algal cultivation was also tested. consecutive treatment with 8.5 mm cacl(2) and 0.2 mm fecl(3) as coagulants and 1% bioflocculant from the culture broth of p. polymyxa am49 showed the highest flocculating activity of up to 95% for high density alga ... | 2010 | 21094603 |
| [fusion expression of fibrinolytic enzyme gene ppfe-i from endophytic paenibacillus polymyxa in escherichia coli and activity analysis]. | with the genomic dna of strain ejs-3 as the template, we amplified the gene of fibrinolytic enzyme from paenibacillus polymyxa (ppfe-i) by pcr. we purified the pcr product and ligated it into pmd19-t. after dna sequencing, we cloned the ppfe-i gene into expression vector pet-dsba and transformed it into escherichia coli bl21(de3). upon induction of iptg, we found that the activity of recombinant fibrinolytic enzyme fused with dsba expressed in escherichia coli was 228 iu/ml. sds-page analysis sh ... | 2010 | 21090119 |
| phytoremediation of metals from fly ash through bacterial augmentation. | different combinations of four bacterial strains isolated from fly ash were used by us to study their impact on phytoextraction of metals from fly ash by brassica juncea grown in fly ash amended with farm yard manure (50:50 w/w). out of 11 bacterial consortia, a combination of two strains i.e. paenibacillus macerans nbrft5 + bacillus pumilus nbrft9 (c7) inoculated in the rhizosphere was found to enhance pb accumulation maximally by 278%, mn by 75%, zn by 163%, cr by 226% and ni by 414% compared ... | 2010 | 21080221 |
| identification of β-propeller phytase-encoding genes in culturable paenibacillus and bacillus spp. from the rhizosphere of pasture plants on volcanic soils. | phytate is one of the most abundant sources of organic phosphorus (p) in soils, but must be mineralized by phytase-producing bacteria to release p for plant uptake. microbial inoculants based on bacillus spp. have been developed commercially, but few studies have evaluated the ecology of these bacteria in the rhizosphere or the types of enzymes that they produce. here, we studied the diversity of aerobic endospore-forming bacteria (efb) with the ability to mineralize phytate in the rhizosphere o ... | 2010 | 21073489 |
| characterization of paenibacillus curdlanolyticus intracellular xylanase xyn10b encoded by the xyn10b gene. | the paenibacillus curdlanolyticus xyn10b gene encoding a family-10 xylanase was cloned and expressed in escherichia coli, and the recombinant enzyme rxyn10b was characterized. immunological analysis suggested that xyn10b is an intracellular enzyme. rxyn10b hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. its properties were significantly different from those of some homologous enzymes. | 2010 | 21071839 |
| novel sigmab regulation modules of gram-positive bacteria involve the use of complex hybrid histidine kinases. | a common bacterial strategy to cope with stressful conditions is the activation of alternative sigma factors that control specific regulons enabling targeted responses. in the human pathogen bacillus cereus, activation of the major stress-responsive sigma factor σ(b) is controlled by a signalling route that involves the multi-sensor hybrid histidine kinase rsbk. rsbk-type kinases are not restricted to the b. cereus group, but occur in a wide variety of other bacterial species, including members ... | 2010 | 21051490 |
| purification and characterization of chitinase from paenibacillus sp. d1. | a 56.56-kda extracellular chitinase from paenibacillus sp. d1 was purified to 52.3-fold by ion exchange chromatography using sp sepharose. maximum enzyme activity was recorded at ph 5.0 and 50 °c. maldi-lc-ms/ms analysis identified the purified enzyme as chitinase with 60% similarity to chitinase chi55 of paenibacillus ehimensis. the activation energy (e (a)) for chitin hydrolysis and temperature quotient (q (10)) at optimum temperature was found to be 19.14 kj/mol and 1.25, respectively. determ ... | 2010 | 21049291 |
| complete genome sequence of paenibacillus polymyxa sc2, a strain of plant growth-promoting rhizobacterium with broad-spectrum antimicrobial activity. | paenibacillus polymyxa sc2 is an important plant growth-promoting rhizobacterium (pgpr). here, we report the complete genome sequence of p. polymyxa sc2. multiple sets of functional genes have been found in the genome. as far as we know, this is the first complete genome sequence of paenibacillus polymyxa. | 2010 | 21037012 |
| paenibacillus algorifonticola sp. nov., isolated from a cold spring in china. | a gram-positive, endospore-forming, rod-shaped bacterium, designated xj259t was isolated from a cold spring sample from xinjiang uyghur autonomous region, china. the isolate grew optimally at 20-30 °c and ph 7.3-7.8. comparative analysis of the 16s rrna gene sequence showed that isolate xj259t phylogenetically belonged to the genus paenibacillus, and most closely related to paenibacillus xinjiangensis b538t (with 96.6 % similarity), paenibacillus glycanilyticus ds-1t (96.3 %) and paenibacillus c ... | 2010 | 20889764 |
| bacterial community diversity in the brazilian atlantic forest soils. | the aim of this study was to characterize the bacterial community diversity of the brazilian atlantic forest soil by means of both cultivation and 16s rrna clone libraries. a collection of 86 representative isolates, obtained from six samples of atlantic forest soils from the national park of serra dos órgãos (parnaso), belonged to the genera arthrobacter, bacillus, burkholderia, leifsonia, paenibacillus, pseudomonas, ralstonia, serratia, and streptomyces according to the 16s rrna sequences. rep ... | 2010 | 20886336 |
| isolation of tn1546-like elements in vancomycin-resistant enterococcus faecium isolated from wood frogs: an emerging risk for zoonotic bacterial infections to humans. | isolation and characterization of vancomycin-resistant enterococci (vre), mainly enterococcus faecium, from the faecal pellet of wood frogs (rana sylvatica). | 2010 | 20880145 |
| reclassification of paenibacillus ginsengisoli as a later heterotypic synonym of paenibacillus anaericanus. | the type strains of the species paenibacillus ginsengisoli (kctc 13931t) and paenibacillus anaericanus (dsm 15890t) were compared in order to clarify the taxonomic relationship of the two species. on the basis of 16s rrna, gyrb and rpob gene sequence comparisons, the two strains shared 99.9, 100 and 99.4 % similarity, respectively. the mean dna-dna relatedness value was 77 % and the genomic dna g+c contents were 43.2 and 42.2 mol%, respectively. phenotypic data, including fatty acid patterns and ... | 2010 | 20870883 |
| stimulation of defense reactions in medicago truncatula by antagonistic lipopeptides from paenibacillus sp. strain b2. | with the aim of obtaining new strategies to control plant diseases, we investigated the ability of antagonistic lipopolypeptides (paenimyxin) from paenibacillus sp. strain b2 to elicit hydrogen peroxide (h₂o₂) production and several defense-related genes in the model legume medicago truncatula. for this purpose, m. truncatula cell suspensions were used and a pathosystem between m. truncatula and fusarium acuminatum was established. in m. truncatula cell cultures, the induction of h₂o₂ reached a ... | 2010 | 20870792 |
| evidence of the involvement of asparagine deamidation in the formation of cyclodextrin glycosyltransferase isoforms in paenibacillus sp. rb01. | cyclodextrin glycosyltransferase (cgtase) from paenibacillus sp. rb01 and its recombinant enzyme exhibit three isoforms (i, ii, and iii) with the same apparent size but different charge. here, we demonstrate for the first time that the deamidation of labile asns causes the change in molecular forms of cgtase. the faster increase in number of isoforms was observed upon incubation in deamidation buffer at the more alkaline ph. the increase in levels of isoform ii and iii over time correlated with ... | 2011 | 20865347 |
| genome sequence of the polymyxin-producing plant-probiotic rhizobacterium paenibacillus polymyxa e681. | paenibacillus polymyxa e681, a spore-forming, low-g+c, gram-positive bacterium isolated from the rhizosphere of winter barley grown in south korea, has great potential for agricultural applications due to its ability to promote plant growth and suppress plant diseases. here we present the complete genome sequence of p. polymyxa e681. its 5.4-mb genome encodes functions specialized to the plant-associated lifestyle and characteristics that are beneficial to plants, such as the production of a pla ... | 2010 | 20851896 |
| interactive optimization of biosurfactant production by paenibacillus alvei arn63 isolated from an iranian oil well. | the potential of an indigenous bacterial strain isolated from an iranian oil field for the production of biosurfactant was investigated in this study. after isolation, the bacterium was characterized to be paenibacillus alvei by biochemical tests and 16s ribotyping. the biosurfactant, which was produced by this bacterium, was able to lower the surface tension of media to 35 mn/m. accordingly, thin layer chromatography (tlc) and ft-ir has been carried out to determine compositional analysis of th ... | 2011 | 20846835 |
| 1h,1'h-[3,3']biindolyl from the terrestrial fungus gliocladium catenulatum. | 2010 | 20823893 | |
| [screening and identification of plant growth-promoting rhizobacteria]. | the interaction between plant growth promoting rhizobacteria (pgpr) and plants can be unstable, pgpr with pgp activities may be well adapted to particular soil environment. based on this, we isolated and identified pgprs from different rhizosphere soils according to their multiple mechanisms. | 2010 | 20815230 |
| characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity--description of paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room. | in the course of studying the influence of n-fertilization on n(2) and n(2)o flux rates in relation to soil bacterial community composition of a long-term fertilization experiment in fen peat grassland, a strain group was isolated that was related to a strain isolated from a spacecraft assembly clean room during diversity studies of microorganisms, which withstood cleaning and bioburden reduction strategies. both the fen soil isolates and the clean room strain revealed versatile physiological ca ... | 2010 | 20813476 |
| purification and characterization of a novel anticoagulant and fibrinolytic enzyme produced by endophytic bacterium paenibacillus polymyxa ejs-3. | endophytes may become a new source of thrombolytic agents for thrombosis treatment. | 2010 | 20813399 |
| ammonium production during the nitrogen-fixing process by wild paenibacillus strains and cell-free extract adsorbed on nano tio₂ particles. | during the nitrogen-fixing process, ammonia (nh₃) is incorporated into glutamate to yield glutamine and is generally not secreted. however, in this study, nh₃- excreting strains of nitrogen-fixing paenibacillus were isolated from soil. the ammonium production by the paenibacillus strains was assayed in different experiments (dry biomass, wet biomass, cell-free extract, and cell-free extract adsorbed on nano tio₂ particles) inside an innovative bioreactor containing capsules of n₂ and h₂. in addi ... | 2010 | 20798591 |
| a novel light-dependent selection marker system in plants. | photosensitizers are common in nature and play diverse roles as defense compounds and pathogenicity determinants and as important molecules in many biological processes. toxoflavin, a photosensitizer produced by burkholderia glumae, has been implicated as an essential virulence factor causing bacterial rice grain rot. toxoflavin produces superoxide and h₂o₂ during redox cycles under oxygen and light, and these reactive oxygen species cause phytotoxic effects. to utilize toxoflavin as a selection ... | 2011 | 20731786 |
| use of cellulases and recombinant cellulose binding domains for refining tcf kraft pulp. | the modular endoglucanase cel9b from paenibacillus barcinonensis is a highly efficient biocatalyst, which expedites pulp refining and reduces the associated energy costs as a result. in this work, we set out to identify the specific structural domain or domains responsible for the action of this enzyme on cellulose fibre surfaces with a view to facilitating the development of new cellulases for optimum biorefining. using the recombinant enzymes gh9-cbd3c, fn3-cbd3b, and cbd3b, which are truncate ... | 2010 | 20730755 |
| identification, cloning and expression of a cold-active beta-galactosidase from a novel arctic bacterium, alkalilactibacillus ikkense. | a novel, cold-active beta-galactosidase was isolated from an arctic gram-positive bacterium, alkalilactibacillus ikkense. the corresponding gene was cloned and expressed as an active enzyme in escherichia coli. denaturing gel electrophoresis of both the native and the recombinant beta-galactosidase showed a monomeric molecular weight of 115-120 kda. analysis of the dna sequence showed sequence similarity to known glycosyl hydrolase family 2 beta-galactosidases from the genera bacillus, paenibaci ... | 2010 | 20718293 |
| production of folate by bacteria isolated from oat bran. | twenty bacteria isolated from three commercial oat bran products were tested for their folate production capability. the bacteria as well as some reference organisms were grown until early stationary phase on a rich medium (ypd), and the amount of total folate in the separated cell mass and the culture medium (supernatant) was determined by microbiological assay. folate vitamer distribution was determined for eight bacteria including one isolated from rye flakes. for seven bacteria the effect of ... | 2010 | 20708290 |
| evaluation of metal ions (zn(2+), fe(3+) and mg(2+)) effect on the production of fusaricidin-type antifungal compounds by paenibacillus polymyxa sqr-21. | effect of metal ions (mg(2+), zn(2+) and fe(3+)) on the production of fusaricidin-type antifungal compounds by paenibacillus polymyxa sqr-21 was studied in liquid culture. first, one-factor; three-level experiments were conducted to find out optimal concentrations of each metal ion for maximum production of fusaricidins. later, three-factor; five-level experiments were performed and a quadratic predictive model was developed using response surface methodology (rsm). the results indicated that fe ... | 2010 | 20685115 |
| monitoring of bacterial communities during low temperature thermal treatment of activated sludge combining dna phylochip and respirometry techniques. | sludge reduction is one of the major challenges in biological wastewater treatment. one approach is to increase the sludge degradation yield together with the biodegradation kinetics. among the various sludge pretreatment strategies proposed, thermal pretreatment at around 65 °c was described as promising. the enhancement in the biodegradation activity due to the selection of thermophilic hydrolytic bacteria was proposed, but further experiments are needed to demonstrate the specific role of the ... | 2010 | 20673948 |
| paleic acid, a fatty acid from paenibacillus sp.: taxonomy, fermentation, isolation, structure determination, and anti-mannheimia and -pasteurella activity. | paleic acid (1), an antibiotic, was obtained from a fermentation broth of paenibacillus sp. bmk771-af3. the compound is a fatty acid (9z,16r)-16-hydroxy-9-octadecenoic acid ((r)-16-hydroxyoleic acid), whose isolation required protection of its polar functional groups. mosher esters of paleic acid yielded information on the absolute configuration of secondary alcohol, and well-resolved (1)h nmr peaks around the double bond suggested that olefin adopted a z geometry. paleic acid showed potent anti ... | 2010 | 20664607 |
| isolation and characterization of bacteriophage phibp from paenibacillus polymyxa ccm 7400. | a bacteriophage phibp infecting paenibacillus polymyxa ccm 7400 was isolated from culture lysate. electron microscopy of lysate samples revealed the presence of bacteriophage particles with polyhedral heads 56 nm in diameter and flexible noncontractile tails 144 nm in length. the profile of phibp structural proteins resembles that of other bacteriophages. the phibp genome consists of double-stranded dna of 43-kbp size. homology search of sequenced dna fragments from ecori digest revealed regions ... | 2010 | 20659166 |
| bioactivity of rosmarinus officinalis essential oils against apis mellifera, varroa destructor and paenibacillus larvae related to the drying treatment of the plant material. | in this study, chemical composition, physicochemical properties and bioactivity of two essential oils of rosmarinus officinalis extracted from plant material with different drying treatments against apis mellifera, varroa destructor and paenibacillus larvae were assessed. the lethal concentration 50 (lc50) for mites and bees was estimated using a complete exposure method test. the broth microdilution method was followed in order to determine the minimum inhibitory concentrations (mics) of the es ... | 2011 | 20623426 |
| mutanase from paenibacillus sp. mp-1 produced inductively by fungal α-1,3-glucan and its potential for the degradation of mutan and streptococcus mutans biofilm. | laetiporus sulphureus is a source of α-1,3-glucan that can substitute for the commercially-unavailable streptococcal mutan used to induce microbial mutanases. the water-insoluble fraction of its fruiting bodies from 0.15 to 0.2% (w/v) induced mutanase activity in paenibacillus sp. mp-1 at 0.35 μ ml(-1). the mutanase extensively hydrolyzed streptococcal mutan, giving 23% of saccharification, and 83% of solubilization of glucan after 6 h. it also degraded α-1,3-polymers of biofilms, formed in vitr ... | 2010 | 20623316 |
| expression and characterization of a novel deoxyribose 5-phosphate aldolase from paenibacillus sp. ea001. | a novel deoc gene was identified from paenibacillus sp. ea001 isolated from soil. the gene had an open reading frame (orf) of 663 base pairs encoding 220 amino acids with a molecular mass of 24.5 kda. the amino acid sequence was 79 % identical to that of deoxyribose 5-phosphate aldolase (dera) from geobacillus sp. y412mc10. the deoc gene encoding dera was cloned into expression vector and the protein was expressed in escherichia coli. the recombinant dera was purified by using ni-nta affinity ch ... | 2010 | 20622498 |
| characterization of two paenibacillus amylolyticus strain 27c64 pectate lyases with activity on highly methylated pectin. | two pectate lyases were identified from paenibacillus amylolyticus 27c64; both enzymes demonstrated activity on methylated pectin in addition to polygalacturonic acid. pela is in a subclass of the pectate lyase family iii. pelb shows some features of pectate lyase family i but is highly divergent. | 2010 | 20622125 |
| anaerobic acidogenic digestion of olive mill wastewaters in biofilm reactors packed with ceramic filters or granular activated carbon. | four identically configured anaerobic packed bed biofilm reactors were developed and employed in the continuous acidogenic digestion of olive mill wastewaters to produce volatile fatty acids (vfas), which can be exploited in the biotechnological production of polyhydroxyalkanoates. ceramic porous cubes or granular activated carbon were used as biofilm supports. aside packing material, the role of temperature and organic loading rate (olr) on vfa production yield and mixture composition were also ... | 2010 | 20619436 |
| isolation and partial characterization of antibiotics produced by paenibacillus elgii b69. | a newly isolated strain b69 with broad antimicrobial activity was identified as paenibacillus elgii by 16s rrna gene sequence analysis, along with physiological and biochemical characterization. two antimicrobial compounds, named as pelgipeptins a and b, were isolated from the culture medium using mci gel chp20p column chromatography and hplc methods. the molecular masses of pelgipeptins a and b were 1072 and 1100 da, respectively. the esi-cid-ms and amino acid analysis suggested that both of th ... | 2010 | 20618851 |
| novel intracellular gh10 xylanase from cohnella laeviribosi hy-21: biocatalytic properties and alterations of substrate specificities by site-directed mutagenesis of trp residues. | the novel intracellular gh10 xylanase (ixylc) gene (1023-bp) of cohnella laeviribosi hy-21 encoded a protein consisting of 340 amino acids with a deduced molecular mass of 39,330da and a calculated pi of 5.81. the primary structure of ixylc was 70% identical to that of geobacillus sp. gh10 enzyme (genbank accession number: edv78425). xylanolytic activity of the his-tagged ixylc overproduced in escherichiacoli bl21 was stimulated by 2.2-fold in the presence of 0.5% non-ionic detergents. ixylc pro ... | 2010 | 20615688 |
| paenibacillus larvae enolase as a virulence factor in honeybee larvae infection. | paenibacillus larvae is a gram-positive spore-forming bacteria, causative agent of american foulbrood (afb), a severe disease affecting larvae of the honeybee apis mellifera. in an attempt to detect potential virulence factors secreted by p. larvae, we identified an enolase among different secreted proteins. although this protein is a cytosolic enzyme involved in glycolytic pathways, it has been related to virulence. the aim of the present work was to evaluate its role during the infection of ho ... | 2011 | 20609532 |
| paenibacillus jilunlii sp. nov., a nitrogen-fixing species isolated from the rhizosphere of begonia semperflorens. | a nitrogen-fixing bacterium, designated strain be17(t), was isolated from rhizosphere soil of begonia semperflorens planted in beijing botanical garden, pr china. phylogenetic analyses based on a segment of the nifh gene sequence and a full-length 16s rrna gene sequence revealed that strain be17(t) was a member of the genus paenibacillus. high levels of 16s rrna gene sequence similarity were found between strain be17(t) and paenibacillus graminis rsa19(t) (97.9 %), paenibacillus sonchi lmg 24727 ... | 2010 | 20601486 |
| expression and characterization of a fusion protein-containing cyclodextrin glycosyltransferase from paenibacillus sp. a11. | a recombinant cyclodextrin glycosyltransferase (cgtase) gene fused with thioredoxin (trx), hexa-histidine (his(6)) and s-protein (s) at the n terminus and a proline-rich peptide (prp) at the c terminus, was constructed using the wild-type gene from paenibacillus sp. a11, the pet-32a vector and escherichia coli bl21(de3) as the host cell. the expression levels and enzyme characteristics of the trx-his(6)-cgtase-prp fusion protein, the recombinant cgtase without fusion peptides, and the wild-type ... | 2010 | 20586059 |
| paenibacillus phoenicis sp. nov., isolated from the phoenix lander assembly facility and a subsurface molybdenum mine. | a novel gram-positive, motile, endospore-forming, aerobic bacterium was isolated from the nasa phoenix lander assembly clean room that exhibits 100 % 16s rrna gene sequence similarity to two strains isolated from a deep subsurface environment. all strains are rod-shaped, endospore-forming bacteria, whose endospores are resistant to uv radiation up to 500 j m(-2). a polyphasic taxonomic study including traditional phenotypic tests, fatty acid analysis, 16s rrna gene sequencing and dna-dna hybridi ... | 2010 | 20584815 |
| production of volatile metabolites by grape-associated microorganisms. | plant-associated microorganisms fulfill important functions for their hosts. whereas promotion of plant growth and health is well-studied, little is known about the impact of microorganisms on plant or fruit flavor. to analyze the production of volatiles of grape-associated microorganisms, samples of grapes of the red cultivar 'blaufraenkisch' were taken during harvest time from four different vineyards in burgenland (austria). the production of volatiles was analyzed for the total culturable mi ... | 2010 | 20575540 |
| [isolation, identification and insecticidal activity of endophyte from achnatherum inebrians]. | to study endophyte species of achnatherum inebrians and to screen strains with insecticidal activity against cotton insect. | 2010 | 20560358 |
| cloning, sequencing and expression analysis of a gene encoding alcohol oxidase in paenibacillus sp. aiu 311. | we have cloned a gene encoding an alcohol oxidase (aod) specific to aldehyde alcohols from paenibacillus sp. aiu 311. the aod gene contains an open reading frame consisting of 618 nucleotides corresponding to 205 amino acid residues. the deduced amino acid sequence exhibits a high similarity to that of manganese superoxide dismutases (sods). we expressed the cloned gene as an active product in escherichia coli bl21 cells. the productivity (total units per culture broth volume) of the recombinant ... | 2010 | 20547358 |
| isolation and identification of bacillus spp. and related genera from different starchy foods. | samples of butternut squash, potatoes, rice, and wheat flour were analyzed. bacillus spp. and related species belonging to paenibacillus and brevibacillus genera were found in 96% of the samples. in butternut squash, predominant species were bacillus pumilus and paenibacillus polymyxa together with other bacillus spp. species (b. cereus, b. licheniformis, b. sphaericus, and b. subtilis). in all the potato samples, bacillus species were detected (b. cereus, b. mycoides, and b. licheniformis). als ... | 2010 | 20546413 |
| lethal infection thresholds of paenibacillus larvae for honeybee drone and worker larvae (apis mellifera). | we compared the mortality of honeybee (apis mellifera) drone and worker larvae from a single queen under controlled in vitro conditions following infection with paenibacillus larvae, a bacterium causing the brood disease american foulbrood (afb). we also determined absolute p. larvae cell numbers and lethal titres in deceased individuals of both sexes up to 8 days post infection using quantitative real-time pcr (qpcr). our results show that in drones the onset of infection induced mortality is d ... | 2010 | 20545737 |
| paenibacillus tianmuensis sp. nov., isolated from soil. | two closely related, gram-stain-negative, rod-shaped, spore-forming strains, b27(t) and f6-b70, were isolated from soil samples of tianmu mountain national natural reserve in zhejiang, china. phylogenetic analysis based on 16s rrna gene and rpob sequences indicated that the isolates were members of the genus paenibacillus. both isolates were closely related to paenibacillus ehimensis ifo 15659(t), paenibacillus elgii sd17(t) and paenibacillus koreensis yc300(t) (≥95.2 % 16s rrna gene sequence si ... | 2011 | 20543152 |
| nodulation in black locust by the gammaproteobacteria pseudomonas sp. and the betaproteobacteria burkholderia sp. | nodulation abilities of bacteria in the subclasses gammaproteobacteria and betaproteobacteria on black locust (robinia pseudoacacia) were tested. pseudomonas sp., burkholderia sp., klebsiella sp., and paenibacillus sp. were isolated from surface-sterilized black locust nodules, but their nodulation ability is unknown. the aims of this study were to determine if these bacteria are symbiotic. the species and genera of the strains were determined by rflp analysis and dna sequencing of 16s rrna gene ... | 2010 | 20542651 |
| characterization and pulp refining activity of a paenibacillus campinasensis cellulase expressed in escherichia coli. | the cel-bl11 gene from paenibacillus campinasensis bl11 was cloned and expressed in escherichia coli as a his-tag fusion protein. zymographic analysis of the recombinant protein revealed cellulase activity corresponding to a protein with a 38-kda molecular weight. the optimum temperature and ph for purified cellulase were 60 degrees c and ph 7.0, respectively. the enzyme retained more than 80% activity after 8h at 60 degrees c at ph 6 and 7. the cellulase has a k(m) of 11.25mg/ml and a v(max) of ... | 2010 | 20541400 |
| indole-3-acetic acid (iaa) production in symbiotic and non-symbiotic nitrogen-fixing bacteria and its optimization by taguchi design. | production of indole-3-acetic acid (iaa) in 35 different symbiotic and non-symbiotic nitrogen-fixing bacteria strains isolated from soil and plant roots was studied and assayed by chromatography and colorimetric methods. these bacteria included agrobacterium, paenibacillus, rhizobium, klebsiella oxytoca, and azotobacter. the best general medium and synergism effects of isolates for iaa production were investigated. effects of different variables containing physical parameters and key media compo ... | 2010 | 20526603 |
| cell surface display of chimeric glycoproteins via the s-layer of paenibacillus alvei. | the gram-positive, mesophilic bacterium paenibacillus alvei ccm 2051(t) possesses a two-dimensional crystalline protein surface layer (s-layer) with oblique lattice symmetry composed of a single type of o-glycoprotein species. herein, we describe a strategy for nanopatterned in vivo cell surface co-display of peptide and glycan epitopes based on this s-layer glycoprotein self-assembly system. the open reading frame of the corresponding structural gene spaa codes for a protein of 983 amino acids, ... | 2010 | 20513375 |
| immunomodulatory activities and subacute toxicity of a novel β-glucan from paenibacillus polymyxa jb115 in rats. | subacute toxicity and immunopharmacological activities of β-glucan from p. polymyxa jb115 was evaluated in a 28-day feeding study in rats. the white blood cell count, red blood cell count, hematocrit, hemoglobin, thrombocytes (thr) and thrombocytocrit were significantly higher in male fed with β-glucan than control rats and the insignificant lower eosinophil count, mean corpuscular volume, mean cell hemoglobin and uninfected thr (uthr) levels were observed in male whereas no marked changes in fe ... | 2011 | 20500124 |
| cloning and characterization of a xylanase, krict px1 from the strain paenibacillus sp. hpl-001. | the krict px1 gene (gb: fj380951) consisting of 996bp encoding a protein of 332 amino acids (38.1kda) from the recently isolated paenibacillus sp. strain hpl-001 (kctc11365bp) has been cloned and expressed in escherichia coli. the xylanase krict px1 showed high activity on birchwood xylan, and was active over a ph range of 5.0 to 11.0, with two optima at ph 5.5 and 9.5 at 50 degrees c with k(m) value of 5.35 and 3.23, respectively. the xylanase activity was not affected by most salts, such as na ... | 2010 | 20493247 |
| optimization of medium for one-step fermentation of inulin extract from jerusalem artichoke tubers using paenibacillus polymyxa zj-9 to produce r,r-2,3-butanediol. | the medium for one-step fermentation of raw inulin extract from jerusalem artichoke tubers by paenibacillus polymyxa zj-9 to produce r,r-2,3-butanediol (r,r-2,3-bd) was developed. inulin, k(2)hpo(4) and nh(4)cl were found to be the key factors in the fermentation according to the results obtained from the plackett-burman experimental design. the optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated acco ... | 2010 | 20452206 |
| antibacterial properties of grapefruit seed extract against paenibacillus larvae subsp. larvae. | twenty-one samples of grapefruit seed extract (gse) either from marketed products or provided by an apiculturist were analysed to verify their inhibition activity, in particular against paenibacillus larvae subsp. larvae, responsible for american foulbrood. the bactericide capacity of gse has been measured in bacillus subtilis bga, bacillus cereus 11778, bacillus cereus k250 and micrococcus luteus 9341a; these bacteria are normally used in the laboratory to study inhibitors. the results showed t ... | 2004 | 20437392 |
| paenibacillus sophorae sp. nov., a nitrogen-fixing species isolated from the rhizosphere of sophora japonica. | a nitrogen-fixing bacterium, designated strain s27(t), was isolated from rhizosphere soil of sophora japonica. phylogenetic analysis based on a fragment of the nifh gene and the full-length 16s rrna gene sequence revealed that strain s27(t) is a member of the genus paenibacillus. high levels of 16s rrna gene sequence similarity were found between strain s27(t) and paenibacillus durus dsm 1735(t) (97.3 %), paenibacillus sabinae dsm 17841(t) (96.9 %), paenibacillus forsythiae dsm 17842(t) (96.7 %) ... | 2011 | 20435753 |
| paenibacillus puldeungensis sp. nov., isolated from a grassy sandbank. | the taxonomic position of a gram-stain-positive, rod-shaped, endospore-forming, facultatively anaerobic bacterial strain, cau 9324(t), isolated from a grassy sandbank was investigated by using a polyphasic approach. strain cau 9324(t) grew optimally at 30 °c and ph 6.0. the cell-wall peptidoglycan contained meso-diaminopimelic acid. the major isoprenoid quinone was menaquinone-7 (mk-7). the polar lipid profile consisted of diphosphatidylglycerol as the major component. the predominant cellular f ... | 2011 | 20418417 |
| biochemical characterization of gram-positive and gram-negative plant-associated bacteria with micro-raman spectroscopy. | raman spectra of gram-positive and gram-negative plant bacteria have been measured with micro-raman spectrometers equipped with 785 and 514.5 nm lasers. the gram-positive bacteria microbacterium testaceum, paenibacillus validus, and clavibacter michiganensis subsp. michiganensis have strong carotenoid bands in the regions 1155-1157 cm(-1) and 1516-1522 cm(-1) that differentiate them from other tested gram-negative bacteria. in the raman spectrum of gram-positive bacteria bacillus megaterium exci ... | 2010 | 20412629 |
| presence of chemical additives and microbial inhibition capacity in grapefruit seed extracts used in apiculture. | american foulbrood, caused by paenibacillus larvae subsp. larvae (white 1906) is one of the most serious diseases of honey bees, causing beekeepers and health workers to make difficult, complex decisions and leading to the development of 'organic' treatments, such as grapefruit seed extract, with minor residue problems in the end product. this study evaluates the chemical composition of grapefruit seed extracts using gas chromatography/mass spectrometry for the detection of benzethonium chloride ... | 2007 | 20411504 |
| in vitro growth-inhibitory effect of plant-derived extracts and compounds against paenibacillus larvae and their acute oral toxicity to adult honey bees. | in total, 26 natural compounds of various chemical classes (flavonoids, alkaloids, terpenoids) and 19 crude extracts from selected plants were tested in vitro for antibacterial activity against three strains of p. larvae, the causal agent of american foulbrood disease of honey bees (afb) by the broth microdilution method. among the individual substances, sanguinarine (mic 4 microg/ml), followed by thymoquinone, capsaicin, trans-2-hexenal and nordihydroguaiaretic acid (mic 4-32 microg/ml) possess ... | 2010 | 20409652 |
| a real-time pcr-based strategy for the detection of paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of american foulbrood. | to develop a real-time pcr-based strategy for the detection of paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of american foulbrood (afb), the most harmful pathology of honeybee brood. | 2010 | 20406378 |
| antibacterial activity of water extracts and essential oils of various aromatic plants against paenibacillus larvae, the causative agent of american foulbrood. | vegetal water extracts, namely the water remaining after hydro-distillation and decoctions, and essential oils of 10 plant species were tested as inhibitors for the growth of paenibacillus larvae, the causative agent of american foulbrood. achyrocline satureioides, chenopodium ambrosioide, eucalyptus cinerea, gnaphalium gaudichaudianum, lippia turbinata, marrubium vulgare,minthostachys verticillata, origanum vulgare, tagetes minuta and thymus vulgaris were included in the study. the water remain ... | 2010 | 20398671 |
| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| paenibacillus sp. strain e18 bifunctional xylanase-glucanase with a single catalytic domain. | xylanases are utilized in a variety of industries for the breakdown of plant materials. most native and engineered bifunctional/multifunctional xylanases have separate catalytic domains within the same polypeptide chain. here we report a new bifunctional xylanase (xynbe18) produced by paenibacillus sp. e18 with xylanase and beta-1,3-1,4-glucanase activities derived from the same active center by substrate competition assays and site-directed mutagenesis of xylanase catalytic glu residues (e129a ... | 2010 | 20382811 |
| paenibacillus rigui sp. nov., isolated from a freshwater wetland. | a rod-shaped, endospore-forming, gram-reaction-variable bacterial strain, designated wpcb173(t), was isolated from freshwater collected from the woopo wetland, republic of korea. based on its phenotypic characteristics and phylogenetic position inferred from 16s rrna gene sequence analysis, the isolate was identified as being a member of the genus paenibacillus. major polar lipids present in strain wpcb173(t) included phosphatidylethanolamine and several unidentified phospholipids. the diamino a ... | 2011 | 20382792 |
| biodegradation of phenol in batch culture by pure and mixed strains of paenibacillus sp. and bacillus cereus. | the bacterial strains, paenibacillus sp. (ay952466) and bacillus cereus (dq002384), have proven capacity to degrade lignin and pentachlorophenol. in the present study, both strains were screened at different concentrations of phenol on mineral salt agar medium in the presence of glucose. at optimized condition (ph 7.5 +/- 0.2, 37 +/- 1 degrees c, 120 rpm, 1% glucose w/v), it was observed that both paenibacillus sp., b. cereus and its mixed culture degraded phenol (500 mg/l) up to 53.86%, 91.63% ... | 2009 | 20380142 |
| cloning and characterization of a novel mannanase from paenibacillus sp. bme-14. | a mannanase gene (man26b) was obtained from a sea bacterium, paenibacillus sp. bme-14, through the constructed genomic library and inverse pcr. the gene of man26b had an open reading frame of 1,428 bp that encoded a peptide of 475- amino acid residues with a calculated molecular mass of 53 kda. man26b possessed two domains, a carbohydrate binding module (cbm) belonging to family 6 and a family 26 catalytic domain (cd) of glycosyl hydrolases, which showed the highest homology to cel44c of p. poly ... | 2010 | 20372022 |
| growth, fe3+ reductase activity, and siderophore production by paenibacillus polymyxa sqr-21 under differential iron conditions. | an experiment was planned to evaluate the behavior of paenibacillus polymyxa sqr-21 under differential iron availability. p. polymyxa was grown under three differential iron conditions (0, 2, 20 μm). iron starvation slowed bacterial growth and at all iron levels, ph of liquid culture was decreased, but maximum decrease was observed at highest iron level. cell surface ferrireductase activity decreased as culture aged, while extracellular fe(3+)-reducing activity constantly increased. hydroxamates ... | 2010 | 20358373 |
| [expression of paenibacillus macerans cyclodextrin glycosyltransferase in pichia pastoris and bacillus subtilis]. | the cgt gene was isolated from paenibacillus macerans by pcr amplification and was inserted into vectors of ppic9k and pmas. the recombinant vectors were transformed to pichia pastoris km71 and bacillus subtilis wb600, respectively. the results showed that alpha-cgtase activity in the culture media of recombinant p pastoris was only 0.2 u/ml, while it was 1.9 u/ml in recombinant b. subtilis. in addition, we optimized the culture conditions of the recombinant b. subtilis strain. after cultivation ... | 2009 | 20352973 |
| effect of bacterial inoculation, plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (solanum tuberosum). | beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner tissues, resulting in endophytic colonization. the major factors contributing to these interactions are not always well understood for most bacterial and plant species. it is believed that specific bacterial functions are required for plant colonization, but also from the plant side specific features are needed, such as plant genotype (cultivar) and developmental stage. v ... | 2010 | 20352404 |
| bacterial community associated with healthy and diseased reef coral mussismilia hispida from eastern brazil. | in order to characterize the bacterial community diversity associated to mucus of the coral mussismilia hispida, four 16s rdna libraries were constructed and 400 clones from each library were analyzed from two healthy colonies, one diseased colony and the surrounding water. nine bacterial phyla were identified in healthy m. hispida, with a dominance of proteobacteria, actinobacteria, acidobacteria, lentisphaerae, and nitrospira. the most commonly found species were related to the genera azospiri ... | 2010 | 20352207 |
| a simple method for the simultaneous decoloration and deproteinization of crude levan extract from paenibacillus polymyxa ejs-3 by macroporous resin. | a simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium paenibacillus polymyxa ejs-3 was developed through static and dynamic adsorption tests of macroporous resins. s-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. under optimized static adsorption conditions (ph 6.0, 35 degrees c and adsorption time of 70 min), the ratios of decoloration, deproteinization and polysaccharid ... | 2010 | 20346649 |
| use of elisa with antiexopolysaccharide antibodies to evaluate wheat-root colonization by the rhizobacterium paenibacillus polymyxa. | enzyme-linked immunosorbent assay with rabbit polyclonal antibodies developed to isolated exopolysaccharide of paenibacillus polymyxa 1465 was used to evaluate the colonization of wheat-seedling roots by this bacterium. the assay conditions were optimized for detection of the p. polymyxa exopolysaccharide determinants forming part of the samples used (homogenates of inoculated roots). the dynamics of the immunoenzymatic revealing of specific polysaccharidic antigenic determinants in the samples' ... | 2010 | 20339848 |
| investigation of factors influencing spore germination of paenibacillus polymyxa accc10252 and sqr-21. | bioorganic fertilizer containing paenibacillus polymyxa sqr-21 showed very good antagonistic activity against fusarium oxysporum. to optimize the role of p. polymyxa sqr-21 in bioorganic fertilizer, we conducted a study of spore germination under various conditions. in this study, l-asparagine, glucose, fructose and k(+) (agfk), and sugars (glucose, fructose, sucrose, and lactose) plus l-alanine were evaluated to determine their ability to induce spore germination of two strains; p. polymyxa acc ... | 2010 | 20309541 |
| lethal protein produced in response to competition between sibling bacterial colonies. | sibling paenibacillus dendritiformis bacterial colonies grown on low-nutrient agar medium mutually inhibit growth through secretion of a lethal factor. analysis of secretions reveals the presence of subtilisin (a protease) and a 12 kda protein, termed sibling lethal factor (slf). purified subtilisin promotes the growth and expansion of p. dendritiformis colonies, whereas slf is lethal and lyses p. dendritiformis cells in culture. slf is encoded by a gene belonging to a large family of bacterial ... | 2010 | 20308591 |
| 16srrna and enzymatic diversity of culturable bacteria from the sediments of oxygen minimum zone in the arabian sea. | sediment underlying the oxygen minimum zone of the eastern arabian sea is rich in organic matter. bacteria in this sediment-water interface are of great ecological importance as they are responsible for decomposing, mineralizing and subsequent recycling of organic matter. this study has for the first time addressed the phylogenetic and functional description of culturable bacteria of this region. genotypic characterization of the isolates using amplified rdna restriction analysis (ardra) followe ... | 2010 | 20229220 |
| paenibacillus chungangensis sp. nov., isolated from a tidal-flat sediment. | a gram-stain-positive, rod-shaped, endospore-forming bacterium, strain cau 9038(t), was isolated from a tidal-flat sediment of daeyijac island, republic of korea, and its taxonomic position was investigated using a polyphasic approach. the cell-wall peptidoglycan contained meso-diaminopimelic acid. the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, the major isoprenoid quinone was mk-7 and the dominant cellular fatty acid was anteiso-c(15 : 0). the dna g+c content was 5 ... | 2011 | 20228214 |
| staphylococcus aureus and zygosaccharomyces bailii as primary microbial contaminants of a spoiled herbal food supplement and evaluation of their survival during shelf life. | this investigation was carried out to identify the microbiota in a spoiled commercial food supplement consisting of a syrup suspension of a mixture of dried herbs and herb extracts. the product did not contain alkyl-p-hydroxybenzoates (parabens) as preservatives, was kept at room temperature and showed abundant gas formation. colonies of distinct morphology were recovered on bacteria- and yeast-specific media, and tested for their ability to grow in the product. genetic differentiation and ident ... | 2010 | 20227600 |
| extracellular expression and biochemical characterization of alpha-cyclodextrin glycosyltransferase from paenibacillus macerans. | the cgt gene encoding alpha-cyclodextrin glycosyltransferase (alpha-cgtase) from paenibacillus macerans strain jfb05-01 was expressed in escherichia coli as a c-terminal his-tagged protein. after 90h of induction, the activity of alpha-cgtase in the culture medium reached 22.5 u/ml, which was approximately 42-fold higher than that from the parent strain. the recombinant alpha-cgtase was purified to homogeneity through either nickel affinity chromatography or a combination of ion-exchange and hyd ... | 2010 | 20227067 |
| a gyrb-targeted pcr for rapid identification of paenibacillus mucilaginosus. | paenibacillus mucilaginosus, one of the typical silicate bacteria, has long been used as a biofertilizer in agriculture and has recently shown potential in bioleaching and wastewater engineering. there has been considerable research involving the isolation of p. mucilaginosus for various utilizations; therefore, rapid identification of this species is of great interest. herein, we describe a specific polymerase chain reaction (pcr) method developed for a rapid identification of p. mucilaginosus, ... | 2010 | 20221758 |
| recombinant expression of an alkali stable gh10 xylanase from paenibacillus barcinonensis. | xylanase a from paenibacillus barcinonensis, a new species isolated from a rice field, has been cloned and expressed in escherichia coli. purified recombinant xylanase showed high activity on xylans from hardwoods and cereals, and exhibited k(m) and v(max) of 2.93 mg/ml and 50.67 u/mg on birchwood xylan. xylanase a was highly active at 60 degrees c in alkaline ph values up to 9.5 and remained stable for at least 3 h in alkaline conditions. the amino acid sequence deduced from xyna revealed that ... | 2010 | 20218604 |
| quantitative polymerase chain reaction (pcr) assays for a bacterial thiaminase i gene and the thiaminase-producing bacterium paenibacillus thiaminolyticus. | the thiaminase i enzyme produced by the gram-positive bacterium paenibacillus thiaminolyticus isolated from the viscera of lake michigan alewives alosa pseudoharengus is currently the only defined source of the thiaminase activity linked to thiamine (vitamin b1) deficiency in early mortality syndrome (ems) in the larvae of great lakes salmonines. diets of alewife or isolated strains of p. thiaminolyticus mixed in a semipurified diet and fed to lake trout salvelinus namaycush have been shown to p ... | 2009 | 20218497 |
| variations of thiaminase i activity ph dependencies among typical great lakes forage fish and paenibacillus thiaminolyticus. | the source of thiaminase in the great lakes food web remains unknown. biochemical characterization of the thiaminase i activities observed in forage fish was undertaken to provide insights into potential thiaminase sources and to optimize catalytic assay conditions. we measured the thiaminase i activities of crude extracts from five forage fish species and one strain of paenibacillus thiaminolyticus over a range of ph values. the clupeids, alewife alosa pseudoharengus and gizzard shad dorosoma c ... | 2009 | 20218495 |
| paenibacillus larvae bacteremia in injection drug users. | paenibacillus larvae causes american foulbrood in honey bees. we describe p. larvae bacteremia in 5 injection drug users who had self-injected honey-prepared methadone proven to contain p. larvae spores. that such preparations may be contaminated with spores of this organism is not well known among pharmacists, physicians, and addicts. | 2010 | 20202425 |
| protein tyrosine o-glycosylation--a rather unexplored prokaryotic glycosylation system. | glycosylation is a frequent and heterogeneous posttranslational protein modification occurring in all domains of life. while protein n-glycosylation at asparagine and o-glycosylation at serine, threonine or hydroxyproline residues have been studied in great detail, only few data are available on o-glycosidic attachment of glycans to the amino acid tyrosine. in this study, we describe the identification and characterization of a bacterial protein tyrosine o-glycosylation system. in the gram-posit ... | 2010 | 20200052 |
| effects of bovine milk lactoperoxidase system on some bacteria. | bovine lactoperoxidase (lpo) was purified from skimmed milk using amberlite cg-50-h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography. lactoperoxidase was purified 20.45-fold with a yield of 28.8%. purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis method and a single band was observed. km was 0.25 mm at 20 degrees c, vmax value was 7.95 micromol/ml min at 20 degrees c (ph 6.0). antibacterial study was done ... | 2010 | 20198919 |
| paenibacillus xylanisolvens sp. nov., a xylan-degrading bacterium from soil. | a xylan-degrading bacterium, strain x11-1(t), was isolated from soil collected in nan province, thailand. the strain was characterized based on its phenotypic and genotypic characteristics. strain x11-1(t) was a gram-stain-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium. it contained meso-diaminopimelic acid in the cell-wall peptidoglycan. the major menaquinone was mk-7, anteiso-c(15 : 0) (56.6 %) and c(16 : 0) (14.0 %) were the predominant cellular fatty acids and diphosp ... | 2011 | 20190026 |
| identification of airborne bacterial and fungal species in the clinical microbiology laboratory of a university teaching hospital employing ribosomal dna (rdna) pcr and gene sequencing techniques. | universal or "broad-range" pcr-based ribosomal dna (rdna) was performed on a collection of 58 isolates (n = 30 bacteria + 28 fungi), originating from environmental air from several locations within a busy clinical microbiology laboratory, supporting a university teaching hospital. a total of 10 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 27/30 (90%) of total bacterial species, consisting of seven genera and include ... | 2009 | 20183192 |
| characterization of a novel lysm domain from lactobacillus fermentum bacteriophage endolysin and its use as an anchor to display heterologous proteins on the surfaces of lactic acid bacteria. | the endolysin lyb5, from lactobacillus fermentum temperate bacteriophage phipyb5, showed a broad lytic spectrum against gram-positive as well as gram-negative bacteria. sequence analysis revealed that the c terminus of the endolysin lyb5 (ly5c) contained three putative lysin motif (lysm) repeat regions, implying that ly5c was involved in bacterial cell wall binding. to investigate the potential of ly5c for surface display, green fluorescent protein (gfp) was fused to ly5c at its n or c terminus ... | 2010 | 20173067 |
| cell surface xylanases of the glycoside hydrolase family 10 are essential for xylan utilization by paenibacillus sp. w-61 as generators of xylo-oligosaccharide inducers for the xylanase genes. | paenibacillus sp. w-61 is capable of utilizing water-insoluble xylan for carbon and energy sources and has three xylanase genes, xyn1, xyn3, and xyn5. xyn1, xyn3, and xyn5 are extracellular enzymes of the glycoside hydrolase (gh) families 11, 30, and 10, respectively. xyn5 contains several domains including those of carbohydrate-binding modules (cbms) similar to a surface-layer homologous (slh) protein. this study focused on the role of xyn5, localized on the cell surface, in water-insoluble xyl ... | 2010 | 20154127 |
| effects of initial inoculation density of paenibacillus polymyxa on colony formation and starch-hydrolytic activity in relation to root rot in ginseng. | to examine the relationships between population growth and biological characters of the plant-growth-promoting rhizobacterium paenibacillus polymyxa gbr-1. | 2010 | 20132376 |