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geographic distribution of the invasive cattle tick rhipicephalus microplus, a country-wide survey in benin.the cattle tick rhipicephalus microplus is currently invading the west african region, and little information is available on the spread of this exotic tick in this region. we set out a country-wide field survey to determine its current distribution in benin. ticks were collected on cattle from 106 farms selected by random sampling covering all regions of the country. rhipicephalus annulatus was found on 70 % of all farms, r. decoloratus on 42 %, r. geigyi on 58 %, and r. microplus on 49 %. ther ...201222689006
exploring the use of an anti-tick vaccine as a tool for the integrated eradication of the cattle fever tick, rhipicephalus (boophilus) annulatus.bovine babesiosis, also known as cattle fever, is a tick-borne protozoal disease foreign to the united states. it was eradicated by eliminating the vector species, rhipicephalus (boophilus) annulatus and rhipicephalus (boophilus) microplus, through the efforts of the cattle fever tick eradication program (cftep), with the exception of a permanent quarantine zone (pqz) in south texas along the border with mexico. keeping the u.s. free of cattle fever ticks in a sustainable manner is a critical na ...201222687762
resistant ticks inhibit metarhizium infection prior to haemocoel invasion by reducing fungal viability on the cuticle surface.we studied disease progression of, and host responses to, four species in the metarhizium anisopliae complex expressing green fluorescent protein (gfp). we compared development and determined their relative levels of virulence against two susceptible arthropods, the cattle tick rhipicephalus annulatus and the lepidopteran galleria mellonella, and two resistant ticks, hyalomma excavatum and rhipicephalus sanguineus. metarhizium brunneum ma7 caused the greatest mortality of r. annulatus, metarhizi ...201222507442
in-vitro assessment of the acaricidal properties of artemisia annua and zataria multiflora essential oils to control cattle ticks.the aim of this study was to investigate the 'acaricidal effect' of zataria multiflora and artemisia annua essential oils on rhipicephalus (boophilus) annulatus.022347275
functional genomics studies of rhipicephalus (boophilus) annulatus ticks in response to infection with the cattle protozoan parasite, babesia bigemina.ticks are obligate haematophagous ectoparasites of wild and domestic animals as well as humans, considered to be second worldwide to mosquitoes as vectors of human diseases, but the most important vectors of disease-causing pathogens in domestic and wild animals. babesia spp. are tick-borne pathogens that cause a disease called babesiosis in a wide range of animals and in humans. in particular, babesia bovis and babesia bigemina are transmitted by cattle ticks, rhipicephalus (boophilus) annulatu ...201222265898
molecular detection of spotted fever group rickettsiae associated with ixodid ticks in egypt.tick-borne diseases comprise a complex epidemiological and ecological network that connects the vectors, pathogens, and a group of host species. the aim of this study was to identify bacteria from the genus rickettsia associated with ixodid ticks infesting camels and cows in egypt. ticks were collected from 6 different localities: qina, giza, qalet el nakhl, new valley, el arish, and minufia, from july to october 2008. species were identified using pcr, followed by sequencing. the glta and rompa ...201222217182
first molecular detection of theileria ovis in rhipicephalus sanguineus tick in iran.to determine tick infestation of domestic ruminants and their infection to ovine theileriosis in northern iran.201222182639
distribution and seasonal activity of tick species on cattle in the west aegean region of turkey.the aim of the present study was to determine the identity, seasonal activity and distribution of tick species of cattle in the west aegean region of turkey between june 2006 and may 2008. nine villages within three provinces, viz. manisa, izmir and aydin, were included in the study and a total of 75 animal barns were visited monthly for a period of 24 months and 443 cattle were examined for the presence of ticks. it was determined that 23% of cattle were infested with ticks. a total of 19,679 a ...201122113777
Control of tick infestations in cattle vaccinated with bacterial membranes containing surface-exposed tick protective antigens.Vaccines containing the Rhipicephalus (Boophilus) microplus BM86 and BM95 antigens protect cattle against tick infestations. Tick subolesin (SUB), elongation factor 1a (EF1a) and ubiquitin (UBQ) are new candidate protective antigens for the control of cattle tick infestations. Previous studies showed that R. microplus BM95 immunogenic peptides fused to the Anaplasma marginale major surface protein (MSP) 1a N-terminal region (BM95-MSP1a) for presentation on the Escherichia coli membrane were prot ...201222085549
expression of heat shock and other stress response proteins in ticks and cultured tick cells in response to anaplasma spp. infection and heat shock.ticks are ectoparasites of animals and humans that serve as vectors of anaplasma and other pathogens that affect humans and animals worldwide. ticks and the pathogens that they transmit have coevolved molecular interactions involving genetic traits of both the tick and the pathogen that mediate their development and survival. in this paper, the expression of heat shock proteins (hsps) and other stress response proteins (srps) was characterized in ticks and cultured tick cells by proteomics and t ...201022084679
acaricidal activity of cassia alata against rhipicephalus (boophilus) annulatus.using adult immersion test, the acaricidal activity of ethanolic extracts of leaves of cassia alata l. was studied against rhipicephalus (boophilus) annulatus. the efficacy was assessed by measuring per cent adult mortality, inhibition of fecundity and hatching rate. the ethanolic extract of c. alata produced a concentration dependant increase in the adult tick mortality. the highest mortality (45.8%) and inhibition of fecundity (10.9%) were observed at the highest concentration tested (100 mg/m ...201221909822
toxic effects of various solvents against rhipicephalus (boophilus) annulatus.the current need of identification of a new acaricidal agent which is acceptable to public as environmentally safe is a daring task. use of herbal acaricides is such an alternative. most of the herbal extracts or fractions are dissolved in polar or non-polar solvents or detergents before tested for acaricidal activity. the solvent or detergent to be used for dissolving the herbal extract should be of little acaricidal activity. in the present study, experimentations were carried out on adult eng ...201121890070
Distribution of Rhipicephalus (Boophilus) microplus and Rhipicephalus (Boophilus) annulatus (Acari: Ixodidae) infestations detected in the United States along the Texas/Mexico border.Species identification and global positioning system coordinates of infestations of cattle fever ticks, Rhipicephalus (Boophilus) annulatus (Say) and Rhipicephalus (Boophilus) microplus (Canestrini), were determined for 790 specimens submitted to the National Veterinary Services Laboratory between 1 October 1999 and 30 September 2010. Cattle fever tick specimens obtained by personnel of the United States Department of Agriculture-Animal and Plant Health Inspection Service-Cattle Fever Tick Eradi ...201121845935
species diversity and geographic distribution of hard ticks (acari: ixodoidea: ixodidae) infesting domestic ruminants, in qazvin province, iran.this report presents the results of the first faunistic study of hard ticks in qazvin province of iran. the primary objective was to determine the species diversity and geographic distribution of hard ticks that parasitize domestic ruminants. information about the abiotic preferences of these species has been provided. a total of 286 cattle, 1,053 goats, and 2,050 sheep were examined in 13 villages in 28 flocks distributed throughout the studied areas. total direct body collections of ticks were ...201121805220
crimean-congo hemorrhagic fever virus in ticks collected from humans, livestock, and picnic sites in the hyperendemic region of turkey.abstract during june and july 2007, about 3125 adult ticks were collected from humans, animals, and vegetation in a hyperendemic region (sivas and tokat) of turkey. a total of 2193 ticks were pooled in 225 pools and screened for the crimean congo hemorrhagic fever virus (cchfv) presence by antigen-capture enzyme-linked immunosorbent assay. infection rates were calculated as the maximum likelihood estimation with 95% confidence intervals (ci). the dominant tick species was found to be hyalomma ma ...201121736490
the hard-tick fauna of mainland portugal (acari: ixodidae): an update on geographical distribution and known associations with hosts and pathogens.this work is an updated revision of the available information on portuguese ixodid tick species. it includes data on tick biology, ecology, taxonomy and host/pathogen-associations. the current list of portuguese ixodid ticks comprises twenty species: dermacentor marginatus (sulzer, 1776), dermacentor reticulatus (fabricius, 1794), haemaphysalis hispanica gil collado, 1938, haemaphysalis inermis birula, 1895, haemaphysalis punctata canestrini & fanzago, 1878, hyalomma lusitanicum koch, 1844, hyal ...201121452063
eclosion blocking effect of ethanolic extract of leucas aspera (lamiaceae) on rhipicephalus (boophilus) annulatus.the crude ethanolic extract of aerial parts of leucas aspera was tested for its acaricidal properties against rhipicephalus (boophilus) annulatus. the per cent adult mortality, inhibition of fecundity and hatching of laid ova were studied at concentrations of 1.56, 3.13, 6.25, 12.5, 25, 50 and 100mg/ml. adult tick mortality was significant at the highest concentration tested. inhibition of fecundity of treated groups differed significantly from control and was concentration dependent. l. aspera ...201121440993
species diversity of ixodid ticks feeding on humans in amasya, turkey: seasonal abundance and presence of crimean-congo hemorrhagic fever virus.ticks (acari:ixodidae) are important pests transmitting tick-borne diseases such as crimean-congo hemorrhagic fever (cchf) to humans. between 2002 and 2009, numerous cchf cases were reported in turkey, including amasya province. in the current study, species diversity, seasonal abundance of ticks, and presence of cchf virus (cchfv) in ticks infesting humans in several districts of amasya province were determined. in the survey, a total of 2,528 ixodid ticks were collected from humans with tick b ...201121337953
rna interference in ticks.ticks are obligate hematophagous ectoparasites of wild and domestic animals and humans, and are considered to be second worldwide to mosquitoes as vectors of human diseases(1) and the most important vectors affecting cattle industry worldwide(2). ticks are classified in the subclass acari, order parasitiformes, suborder ixodida and are distributed worldwide from arctic to tropical regions(3). despite efforts to control tick infestations, these ectoparasites remain a serious problem for human and ...201121304465
identification and characterization of ixodes scapularis antigens that elicit tick immunity using yeast surface display.repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. in this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. we constructed an ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identifie ...201121246036
assessment of bacterial diversity in the cattle tick rhipicephalus (boophilus) microplus through tag-encoded pyrosequencing.ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. the cattle tick, rhipicephalus (boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. tick microbiomes remain largely unexplored. the objective of this study was to explore the r. microplus microbiome by applying the bacterial 16s tag-encoded flx-titanium amplicon pyrosequencing (btefap) technique to characterize its bacterial ...201121211038
the rhipicephalus (boophilus) microplus bm86 gene plays a critical role in the fitness of ticks fed on cattle during acute babesia bovis infection.abstract:201021092112
detection of babesia and theileria species infection in cattle from portugal using a reverse line blotting method.babesiosis and theileriosis are tick-borne diseases widespread in tropical and sub-tropical regions with high economic impact worldwide. in portugal there are at least 4 tick vectors known to be competent for the transmission of babesia and theileria sp. identified: rhipicephalus bursa, rhipicephalus (boophilus) annulatus, ixodes ricinus and haemaphysalis punctata. all these potential babesia and theileria tick vectors are widely distributed in portugal, although they are predominant in the sout ...201021036481
crimean-congo hemorrhagic fever in european part of turkey: genetic analysis of the virus strains from ticks and a seroepidemiological study in humans.abstract a survey of ticks from domestic ruminants, together with a serosurvey in humans was conducted in thrace (northwestern turkey) to evaluate the prevalence of crimean-congo hemorrhagic fever virus (cchfv) in ticks and humans. more prevalent ticks were hyalomma marginatum, hyalomma aegyptium, rhipicephalus bursa, and rhipicephalus (boophilus) annulatus, with low numbers of dermacentor marginatus, rhipicephalus sanguineus group, and ixodes ricinus. no differences in the tick faunal compositi ...201021028961
tick-borne rickettsioses, neglected emerging diseases in rural senegal.rickettsioses are one of the most important causes of systemic febrile illness among travelers from developed countries, but little is known about their incidence in indigenous populations, especially in west africa.201020856858
molecular cloning of a small heat shock protein (shspii) from the cattle tick rhipicephalus (boophilus) annulatus salivary gland.immunoscreening of a cdna expression library of the rhipicephalus (boophilus) annulatus tick with purified rabbit anti-r annulatus salivary glands antigens polyclonal antibodies led to the identification of a 661bp sequence. the sequence includes an open reading frame of 543bp encoding a protein of 180 amino acids with calculated molecular weight of 20.51kda, isoelectric point of 9.071 and with no signal sequence. comparison of the deduced amino acids with protein data bank showed that the ident ...201020723560
application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples.abstract:201020704695
ixodid ticks (acari: ixodidae) infesting humans in tokat province of turkey: species diversity and seasonal activity.ixodid ticks (acari: ixodidae) are the major vectors of pathogens threatening animal and human health. tokat province, turkey, is a suitable habitat for extended tick activity with its moderate climate and vegetation. in the present study, we surveyed humans visiting health centers to determine the species diversity, geographical distribution, and seasonal activity of ixodid ticks infesting them. out of 5,999 adult ticks collected from humans from april to september, 2008, 800 ticks were identif ...201020618665
prevalence of tick-borne pathogens in ticks in sicily.the prevalence of anaplasma, ehrlichia, rickettsia and babesia/theileria species was analysed in questing and feeding adult ticks in sicily. a total of 678 ticks were collected and analysed in this study. of these, 29 were questing ticks and 649 were collected from infested cattle, sheep, goats or dogs. tick species analysed included rhipicephalus bursa, r. turanicus, r. sanguineus, hyalomma lusitanicum, h. marginatum, dermacentor marginatus, ixodes ricinus, r. (boophilus) annulatus and haemaphy ...201020537102
lavandula angustifolia essential oil as a novel and promising natural candidate for tick (rhipicephalus (boophilus) annulatus) control.lavandula angustifolia is a well known herbal medicine with a variety of useful properties, including its acaricidal effect. this experiment was carried out to study the bioacaricidal activity of l. angustifolia essential oil (eo) against engorged rhipicephalus (boophilus) annulatus (acari; ixodidae) females. for this purpose six serial concentrations (0.5, 1.0, 2.0, 4.0, 6.0 and 8.0% w/v) of l. angustifolia eo were used. there was considerable mortality in concentrations more than 4.0% although ...201020433836
evidence for role of white-tailed deer (artiodactyla: cervidae) in epizootiology of cattle ticks and southern cattle ticks (acari: ixodidae) in reinfestations along the texas/mexico border in south texas: a review and update.from 1907 when the fever tick eradication campaign began until 1933, the tick eradication methods of dipping cattle in an acaricide or "pasture vacation" were enormously successful in eradicating southern cattle ticks [rhipicephalus (boophilus) microplus (canestrini)], until failures began to occur in some areas of florida. regarding the failures in florida, the consensus was that populations of white-tailed deer [odocoileus virginianus (zimmermann)] infested with southern cattle ticks were resp ...201020429430
coxiella burnetii in humans and ticks in rural senegal.q fever is a worldwide zoonotic disease caused by coxiella burnetii. epidemiologically, animals are considered reservoirs and humans incidental hosts.201020386603
novel technique for quantifying adhesion of metarhizium anisopliae conidia to the tick cuticle.the present study describes an accurate quantitative method for quantifying the adherence of conidia to the arthropod cuticle and the dynamics of conidial germination on the host. the method was developed using conidia of metarhizium anisopliae var. anisopliae (metschn.) sorokin (hypocreales: clavicipitaceae) and engorged rhipicephalus annulatus (say) (arachnida: ixodidae) females and was also verified for m. anisopliae var. acridum driver et milner (hypocreales: clavicipitaceae) and alphitobius ...201020363785
association of pathogen strain-specific gene transcription and transmission efficiency phenotype of anaplasma marginale.efficient transmission of pathogens by an arthropod vector is influenced by the ability of the pathogen to replicate and develop infectiousness within the arthropod host. while the basic life cycle of development within and transmission from the arthropod vector are known for many bacterial and protozoan pathogens, the determinants of transmission efficiency are largely unknown and represent a significant gap in our knowledge. the st. maries strain of anaplasma marginale is a high-transmission-e ...201020308303
preliminary tests in mexico with ddt, cube, hexachlorocyclohexane (benzene hexachloride) and combinations thereof, for the control of the cattle fever tick, boophilus annulatus. 194720251185
meeting the challenges of on-host and off-host water balance in blood-feeding arthropods.in this review, we describe water balance requirements of blood-feeding arthropods, particularly contrasting dehydration tolerance during the unfed, off-host state and the challenges of excess water that accompany receipt of the bloodmeal. most basic water balance characteristics during the off-host stage are applicable to other terrestrial arthropods, as well. a well-coordinated suite of responses enable arthropods to conserve water resources, enhance their desiccation tolerance, and increase t ...201020206630
biogeography of tick-borne bhanja virus (bunyaviridae) in europe.bhanja virus (bhav) is pathogenic for young domestic ruminants and also for humans, causing fever and affections of the central nervous system. this generally neglected arbovirus of the family bunyaviridae is transmitted by metastriate ticks of the genera haemaphysalis, dermacentor, hyalomma, rhipicephalus, boophilus, and amblyomma. geographic distribution of bhav covers southern and central asia, africa, and southern (partially also central) europe. comparative biogeographic study of eight know ...201020182535
characterization of ferritin 2 for the control of tick infestations.ixodes ricinus is one the most abundant tick species in europe and these ticks transmit pathogens causing human and animal diseases. the cattle ticks, rhipicephalus (boophilus) spp., affect cattle production in tropical and subtropical regions of the world. development of vaccines directed against tick proteins may reduce tick infestations and the transmission of tick-borne pathogens. however, a limiting step in tick vaccine development has been the identification of tick protective antigens. he ...201020171306
selection of reference genes for quantitative rt-pcr studies in rhipicephalus (boophilus) microplus and rhipicephalus appendiculatus ticks and determination of the expression profile of bm86.for accurate and reliable gene expression analysis, normalization of gene expression data against reference genes is essential. in most studies on ticks where (semi-)quantitative rt-pcr is employed, normalization occurs with a single reference gene, usually beta-actin, without validation of its presumed expression stability. the first goal of this study was to evaluate the expression stability of commonly used reference genes in rhipicephalus appendiculatus and rhipicephalus (boophilus) microplu ...200920040102
identification and characterization of rhipicephalus (boophilus) microplus candidate protective antigens for the control of cattle tick infestations.the cattle ticks, rhipicephalus (boophilus) spp., affect cattle production in tropical and subtropical regions of the world. tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. the recombinant rhipicephalus microplus bm86 antigen has been shown to protect cattle against tick infestations. however, variable efficacy of bm86-based vaccines against geographic tick strains has encouraged the research for additional tick-protective antigens. herein, we ...201019943063
the first clinical case due to ap92 like strain of crimean-congo hemorrhagic fever virus and a field survey.crimean-congo hemorrhagic fever (cchf) is a fatal infection, but no clinical case due to ap92 strain was reported. we described the first clinical case due to ap92 like cchfv.200919515251
isolation of entomopathogenic fungi from soils and ixodes scapularis (acari: ixodidae) ticks: prevalence and methods.entomopathogenic fungi are commonly found in forested soils that provide tick habitat, and many species are pathogenic to ixodes scapularis say, the blacklegged tick. as a first step to developing effective biocontrol strategies, the objective of this study was to determine the best methods to isolate entomopathogenic fungal species from field-collected samples of soils and ticks from an eastern deciduous forest where i. scapularis is common. several methods were assessed: (1) soils, leaf litter ...200919496427
pathogenicity of metarhizium anisopliae (hypocreales: clavicipitaceae) to tick eggs and the effect of egg cuticular lipids on conidia development.the ovicidal efficacy of two entomopathogenic hyphomycetes fungi--metarhizium anisopliae variety acridum (m. an. ac.) driver and milner (hypocreales: clavicipitaceae) and metarhizium anisopliae variety anisopliae (m. an. an.) (metschn.) sorokin (hypocreales: clavicipitaceae)--was evaluated against eggs of three tick species (acari: ixodidae)--hyalomma excavatum (koch), rhipicephalus (boophilus) annulatus (say), and rhipicephalus sanguineus (latereille)--by placing eggs, laid by surface-sterilize ...200919496424
[construction of cdna expression library of unfed female haemaphysalis longicornis and immuno-screening].to construct a cdna expression library from unfed female tick haemaphysalis longicornis for screening and cloning potential antigenic genes.200919459491
detection of tick blood parasites in egypt using pcr assay i--babesia bovis and babesia bigemina.babesia bovis and babesia bigemina are distributed all over the world; the etiologic agents of the animal babesiosis are considered the most important tick-borne disease. the present research work was the first attempt to determine the prevalence of b. bovis and b. bigemina infection in ticks, in egypt, by using polymerase chain reaction (pcr). questing 5,243 hard and soft ticks were collected from different localities throughout the giza governorate. furthermore, dna from 500 different individu ...200919415329
acaricidal effect of pelargonium roseum and eucalyptus globulus essential oils against adult stage of rhipicephalus (boophilus) annulatus in vitro.in a laboratory trial, in west-central iran, the acaricidal effects of the essential oils (eos) prepared from two medicinal plants, i.e. pelargonium roseum and eucalyptus globulus on the adult stage of rhipicephalus (boophilus) annulatus were evaluated. for this purpose, the engorged females of r. (b) annulatus were exposed to two-fold serial dilutions of oils (0.31-5.0%) using a "dipping method" in vitro. the engorged ticks were immersed in different plant dilutions (eight per dilution) for 1mi ...200919356854
vaccination with recombinant boophilus annulatus bm86 ortholog protein, ba86, protects cattle against b. annulatus and b. microplus infestations.the cattle ticks, boophilus spp., affect cattle production in tropical and subtropical regions of the world. tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. the recombinant b. microplus bm86 protective antigen has been shown to protect cattle against tick infestations. recently, the gene coding for b. annulatus bm86 ortholog, ba86, was cloned and the recombinant protein was secreted and purified from the yeast pichia pastoris.200919335900
allopatric speciation in ticks: genetic and reproductive divergence between geographic strains of rhipicephalus (boophilus) microplus.the cattle tick, rhipicephalus (boophilus) microplus, economically impact cattle industry in tropical and subtropical regions of the world. the morphological and genetic differences among r. microplus strains have been documented in the literature, suggesting that biogeographical and ecological separation may have resulted in boophilid ticks from america/africa and those from australia being different species. to test the hypothesis of the presence of different boophilid species, herein we perfo ...200919243585
prevalence and genotypes of anaplasma species and habitat suitability for ticks in a mediterranean ecosystem.anaplasma species are tick-transmitted pathogens that impact veterinary and human health. sicily is one of the locations where these pathogens are endemic. sicily represents a typical mediterranean ecosystem to study anaplasma infection and tick habitat suitability. the aims of this study were (i) to characterize by 16s rrna and species-specific msp4 gene pcr the prevalence and genotypes of a. marginale, a. phagocytophilum, and a. ovis in the most abundant host species in sicilian provinces and ...200818978093
experimental transmission of field anaplasma marginale and the a. centrale vaccine strain by hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus ticks.the cattle rickettsia anaplasma marginale is distributed worldwide and is transmitted by about 20 tick species, but only rhipicephalus simus, a strictly african tick species, has been shown to transmit the vaccine strain of a. centrale. the aim of the present study was to examine transmission of field strains of a. marginale and of the vaccine strain of a. centrale by three tick species -hyalomma excavatum, rhipicephalus sanguineus and rhipicephalus (boophilus) annulatus - to susceptible calves. ...200918823724
human babesiosis.human babesiosis is an emerging intraerythrocytic infection caused by protozoal parasites transmitted by ixodid ticks. babesiosis is endemic in the northeastern and upper midwestern regions of the united states and is found sporadically in other parts of the united states, europe, asia, africa, and south america. babesial infections range from asymptomatic to severe and occasionally are fatal. specific laboratory diagnosis of babesial infection is made by morphologic examination of giemsa-staine ...200818755385
reliminary survey of ticks (acari: ixodidae) on cattle in central equatoria state, southern sudan.in a preliminary survey conducted in 2005, the species composition and seasonality of ticks infesting cattle in central equatoria state, southern sudan was determined. three locations were selected (gumbo, khor rumla and nyaing) and surveyed every 3 months. two cattle herds in each of the three locations were visited four times during the study period. total body collections of ticks were made from each of five cattle (nilotic zebu breed) kept in six different herds. four tick genera and ten spe ...200818575063
ticks infesting domestic animals in northern greece.the tick species infesting grazing animals in the countryside of 11 prefectures in northern greece were investigated during april-july and september-december of consecutive years 2003-2006. a total of 3,249 (1,952 males, 1,297 females) adult ticks were collected from goats, sheep, cattle and dogs. ticks were identified as ixodes ricinus (44.57%), ixodes gibbosus (4.09%), rhipicephalus bursa (19.14%), rhipicephalus sanguineus, rhipicephalus turanicus (5.79%), hyalomma marginatum marginatum (12.40 ...200818563592
a molecular survey of theileria and babesia parasites in cattle, with a note on the distribution of ticks in tunisia.between october and november 2006, a total of 278 bovine blood samples were examined, and 104 (37.4%) were positive for piroplasms by microscopy. a reverse line blot hybridisation with polymerase chain reaction detected theileria annulata, t. buffeli, babesia bovis and b. bigemina in cattle accounting for 48.6% of positive samples. the most frequently found species was t. buffeli, which was present in 39.2% of the samples. t. annulata was found in 48 samples (17.3%). babesia infections were less ...200818458949
cellular and molecular characterization of an embryonic cell line (bme26) from the tick rhipicephalus (boophilus) microplus.the cellular and molecular characteristics of a cell line (bme26) derived from embryos of the cattle tick rhipicephalus (boophilus) microplus were studied. the cells contained glycogen inclusions, numerous mitochondria, and vesicles with heterogeneous electron densities dispersed throughout the cytoplasm. vesicles contained lipids and sequestered palladium meso-porphyrin (pd-mp) and rhodamine-hemoglobin, suggesting their involvement in the autophagic and endocytic pathways. the cells phagocytose ...200818405834
expression of recombinant rhipicephalus (boophilus) microplus, r. annulatus and r. decoloratus bm86 orthologs as secreted proteins in pichia pastoris.rhipicephalus (boophilus) spp. ticks economically impact on cattle production in africa and other tropical and subtropical regions of the world. tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. the r. microplus bm86 protective antigen has been produced by recombinant dna technology and shown to protect cattle against tick infestations.200818275601
greek goat encephalitis virus strain isolated from ixodes ricinus, greece.a strain of greek goat encephaltitis virus was isolated from engorged ixodes ricinus ticks that had fed on goats in northern greece. the strain was almost identical to the prototype strain isolated 35 years ago.200818258134
molecular cloning, expression and characterization of a functional gstmu class from the cattle tick boophilus annulatus.a full-length cdna of a glutathione s-transferase (gst) was cloned from a cdna library of the local egyptian cattle tick boophilus annulatus. the 672 bp cloned fragment was sequenced and showed an open reading frame encoding a protein of 223 amino acids. comparison of the deduced amino acid sequence with gsts from other species revealed that the sequence is closely related to the mammalian mu-class gst. the cloned gene was expressed in e. coli under t7 promotor of pet-30b vector, and purified un ...200818241993
competitive exclusion between piroplasmosis and anaplasmosis agents within cattle. 200818225951
prevalence of ixodid ticks on cattle in mazandaran province, iran.a survey was carried out to investigate the prevalence of hard tick species (acari: ixodidae) on cattle in mazandaran province, iran. a total of 953 ticks were collected from 86 infested cattle during activating seasons of ticks during 2004-2005. nine species were identified: boophilus annulatus (51.3%), rhipicephalus bursa (16.8%), haemaphysalis punctata (6.3%), ixodes ricinus (6.8%), hyalomma marginatum (12.5%), hyalomma anatolicum excavatum (5.2%), hyalomma asiaticum (0.6%), hyalomma detritum ...200718165714
molecular cloning and expression of a larval immunogenic protein from the cattle tick boophilus annulatus.a full-length cdna of an immunogenic protein was cloned from a cdna library of the local egyptian cattle tick boophilus annulatus. antibodies raised against b. annulatus larval proteins were used to screen a cdna expression library. a 936bp cloned fragment was sequenced and showed an open reading frame of 516bp encoding a protein of 171 amino acids. comparison of the deduced amino acid sequence with protein data bank revealed that the sequence is related to a sequence isolated from the hard tick ...200818054085
climate niches of tick species in the mediterranean region: modeling of occurrence data, distributional constraints, and impact of climate change.in this study, we used ecological niche factor analysis (enfa) and principal components analysis (pca) of climate variables to define the climate niches and areas of potential colonization of six species of ticks in the mediterranean region: dermacentor marginatus sulzer, rhipicephalus bursa canestrini & fanzago, rhipicephalus turanicus pomerantsev, matikashvili & lototsky, hyalomma marginatum koch, hyalomma excavatum koch, and boophilus annulatus (say). enfa generated distribution models that v ...200718047215
status of tick infestation of cattle in the kayseri region of turkey.this study was carried out to determine the status of tick infestation of cattle in the kayseri region in turkey. eight hundred and sixty-six cattle and their barns in 12 localities were examined during the period of june 2000-november 2001, where 188 (21.7%) of which were infested by ticks. a total of 1,585 ticks consisting of rhipicephalus turanicus (2.27%), r. bursa (2.14%), r. sanguineus (0.94%), hyalomma marginatum (17.16%), h. anatolicum excavatum (24.73%), h. a. anatolicum (19.62%), derma ...200717823821
biological activities of chamomile (matricaria chamomile) flowers' extract against the survival and egg laying of the cattle fever tick (acari ixodidae).in the present work, the potential of acaricidal activity of chamomile flowers' extract was studied against engorged rhipicephalus annulatus tick under laboratory condition. for this purpose, the engorged females of rhipicephalus annulatus were exposed to two-fold serial dilutions of chamomile flowers' extract (0.5%, 1.0%, 2.0%, 4.0% and 8.0%) using "dipping method" in vitro. the engorged ticks were immersed in different plant dilutions (five ticks for each dilution) for 1 min and they were imme ...200717726752
immunologic and molecular identification of babesia bovis and babesia bigemina in free-ranging white-tailed deer in northern mexico.the suitability of white-tailed deer (odocoileus virginianus) as hosts for the cattle ticks rhipicephalus (boophilus) microplus and rhipicephalus (boophilus) annulatus, has been well documented. these ticks have a wide host range, and both transmit babesia bovis and babesia bigemina, the agents responsible for bovine babesiosis. although this disease and its vectors have been eradicated from the united states and some states in northern mexico, it still is a problem in other mexican states. it i ...200717699089
detection of theileria and babesia species in ticks collected from cattle.the present study was carried out to detect tick species that infest cattle, and theileria and babesia species transmitted by these ticks in kayseri province (turkey). a total of 300 cattle were examined for tick infestations. of the 300 cattle, 117 (39%) were infested with ticks. a total of 1160 ticks belonging to 11 ixodid genera were collected from the infested animals and their shelters. the most prevalent tick species was boophilus annulatus 26.37% (306/1160) followed by hyalomma marginatum ...200717614205
conservation of transmission phenotype of anaplasma marginale (rickettsiales: anaplasmataceae) strains among dermacentor and rhipicephalus ticks (acari: ixodidae).before the eradication of boophilus ticks from the united states, rhipicephalus (boophilus) microplus (canestrini) and rhipicephalus (boophilus) annulatus (say) were important biological vectors of the cattle pathogen anaplasma marginale theiler. in the absence of boophilus ticks, a. marginale continues to be transmitted by dermacentor ticks. however, a few u.s. strains are not transmissible by dermacentor andersoni stiles, dermacentor variabilis (say), or both, raising the question of how these ...200717547235
purification and characterization of two larval glycoproteins from the cattle tick, boophilus annulatus.the present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, boophilus annulatus (acari: ixodidae). two specific larval glycoproteins (glps) were isolated by two-step affinity chromatography. the larval immunogens were first purified with cnbr-sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with con-a sepharose. these glycoproteins have molecular weights of approximately 32 and 15 kda with ...200717519572
identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of anaplasma marginale.understanding the determinants of efficient tick-borne microbial transmission is needed to better predict the emergence of highly transmissible pathogen strains and disease outbreaks. although the basic developmental cycle of anaplasma and ehrlichia spp. within the tick has been delineated, there are marked differences in the ability of specific strains to be efficiently tick transmitted. using the highly transmissible st. maries strain of anaplasma marginale in dermacentor andersoni as a positi ...200717420231
babesia bigemina: attenuation of an uzbek isolate for immunization of cattle with live calf- or culture-derived parasites.the virulence of an uzbek isolate of babesia bigemina, obtained from infected boophilus annulatus ticks from an endemic area in uzbekistan, was attenuated for immunization of cattle with autochthonous calf- or culture-derived parasites in uzbekistan. after four "slow passages" in vivo the virulence was reduced, as evidenced by the response of calves inoculated with an experimental live frozen vaccine produced from the following passage. the vaccine was safe and protective against homologous viru ...200717368728
gene silencing of the tick protective antigens, bm86, bm91 and subolesin, in the one-host tick boophilus microplus by rna interference.the use of rna interference (rnai) to assess gene function has been demonstrated in several three-host tick species but adaptation of rnai to the one-host tick, boophilus microplus, has not been reported. we evaluated the application of rnai in b. microplus and the effect of gene silencing on three tick-protective antigens: bm86, bm91 and subolesin. gene-specific double-stranded (dsrna) was injected into two tick stages, freshly molted unfed and engorged females, and specific gene silencing was ...200717196597
biological control of rhipicephalus (boophilus) annulatus by different strains of metarhizium anisopliae, beauveria bassiana and lecanicillium psalliotae fungi.virulence of 11 native strains of entomopathogenic fungi; metarhizium anisopliae (three strains), beauveria bassiana (six strains) and lecanicillium psalliotae (two strains) collected from different parts of iran, were studied against different developmental stages of rhipicephalus (boophilus) annulatus. after the exposure of ticks to the fungal strains in different concentrations (i.e. 10(3), 10(5), 10(7) conidia/ml), various parameters such as mortality rate and reproductive efficiency of engo ...200717186273
[determination of tick species and treatment of cows, sheep and goats in the sivas-zara region].the purpose of this study was to examine tick infestation in cattle, sheep and goats in the zara-sivas region for one year and to determine the epizootiology of the tick species as well as to investigate treatment of the infested animals. tick infestation was detected in 71 (29.6%) out of 240 cattle, 66 (24.0%) out of 275 sheep and 50 (19.9%) out of 252 goats in the zara region. it has been shown that the tick infestation on cattle included haemaphysalis parva (33.8%), dermacentor marginatus (2. ...200617160854
importance of ticks and their chemical and immunological control in livestock.the medical and economic importance of ticks has long been recognized due to their ability to transmit diseases to humans and animals. ticks cause great economic losses to livestock, and adversely affect livestock hosts in several ways. loss of blood is a direct effect of ticks acting as potential vector for haemo-protozoa and helminth parasites. blood sucking by large numbers of ticks causes reduction in live weight and anemia among domestic animals, while their bites also reduce the quality of ...017048307
rickettsial agents in egyptian ticks collected from domestic animals.to assess the presence of rickettsial pathogens in ticks from egypt, we collected ticks from domestic and peridomestic animals between june 2002 and july 2003. dna extracts from 1019 ticks were tested, using pcr and sequencing, for anaplasma spp., bartonella spp., coxiella burnetii, ehrlichia spp., and rickettsia spp. ticks included: 29 argas persicus, 10 hyalomma anatolicum anatolicum, 55 hyalomma anatolicum excavatum, 174 hyalomma dromedarii, 2 hyalomma impeltatum, 3 hyalomma marginatum rufipe ...200617004028
high-resolution predictive mapping for boophilus annulatus and b. microplus (acari: ixodidae) in mexico and southern texas.in this study we use high-resolution satellite imagery to map habitat suitability for both boophilus annulatus and b. microplus over an area covering parts of texas and northeastern mexico. the habitat for these tick species covers widely different regions. b. microplus finds satisfactory abiotic conditions in wet coastal habitats, whereas b. annulatus finds suitable habitat in the drier inner parts of southwestern texas and northern mexico; the two species have very different habitat requiremen ...200616956729
a case of histologically diagnosed tick infestation on the scalp of a korean child.a scalp mass surgically excised from a 4-year-old korean boy was identified as a tick through histological observations. in sections of the mass, characteristic features of a tick, including its gross contour, cuticular structures, well developed musculature and salivary glands, and the capitulum, were discovered. in particular, the capitulum is anteriorly protruded, which strongly suggests that the specimen be a hard tick of family ixodidae. however, the present histological features were not e ...200616809965
ixodid ticks parasitizing iberian red deer (cervus elaphus hispanicus) and european wild boar (sus scrofa) from spain: geographical and temporal distribution.commercial hunting of spanish wild ungulates has made them an important economic resource. wild ungulates may have an important role in the maintenance of ixodid tick populations, and also as reservoirs of pathogens. we studied the ixodid ticks that parasitize iberian red deer and european wild boar from spain. ixodid ticks (n=6,336) were collected from 431 iberian red deer and 142 wild boar in different regions of spain. we found 10 different ixodid tick species parasitizing iberian red deer, m ...200616675125
comparative study of anaplasma parasites in tick carrying buffaloes and cattle.a comparative study on the prevalence of anaplasma parasite was conducted on ticks carrying buffaloes and cattle. five hundred blood samples of both animals (250 of each) were collected during february, march and april. thin blood smears on glass slides were made, fixed in 100% methyl alcohol and examined. microscopic examination revealed that 205 (41%) animals had anaplasma parasites, out of which 89, 44 and 72 animals had anaplasma marginale, anaplasma centrale and mixed infection respectively ...200516252338
[detection of the circulation of crimean-congo hemorrhagic fever virus in the piedmont steppes of the north caucasus].the laboratory verified cases of crimean-congo hemorrhagic fever (cchf) in the piedmont steppes of the north caucasus (malgobeksky district, republic of ingushetia) are first described. the source of the first infection was ixodidae ticks; three subsequent sources were contacts with the bloody discharges from patients. cchf virus genome was detected in the blood of the cattle from an epidemic focus and in the pools of the ixodes ticks haemaphysalis parva neum., 1897 and boophilus annulatus say, ...200516250591
the distribution of ticks (acari: ixodidae) of domestic livestock in portugal.this paper introduces the first countrywide faunistic study of the tick parasites on ruminants in portugal. the aim of this study was to map accurately the distribution of the ticks dermacentor marginatus, rhipicephalus (boophilus) annulatus, r. bursa, hyalomma m. marginatum, h. lusitanicum and ixodes ricinus in portugal. additional information about the abiotic preferences of these species has been obtained through the use of abiotic (temperature- and vegetation-derived) variables have been rec ...200516132737
genetic diversity of anaplasma marginale strains from cattle farms in the province of palermo, sicily.bovine anaplasmosis, caused by the tick-borne rickettsia anaplasma marginale, is endemic in sicily and results in economic loss to the cattle industry. this study was designed to characterize strains of a. marginale at the molecular level from cattle in the province of palermo, sicily. seropositivity of cattle >or=1 year old for a. marginale in the study area ranged from 62% to 100%. the observed prevalence of a. marginale infections in cattle herds ranged from 25% to 100%. two predominant a. ma ...200516115096
monthly dynamics of ticks (acari:ixodida) infesting n'dama cattle in the republic of guinea.the occurrence of ixodid ticks on n'dama cattle was studied in the republic of guinea between june 1994 and may 1995. monthly tick collections were performed on 80 animals from 14 villages located in dabola, kouroussa and dinguiraye prefectures. a total of 19,804 ticks was collected and classified using standard taxonomic keys. the following tick species were identified: amblyomma variegatum, hyalomma marginatum rufipes, hyalomma trunctum, hyalomma nitidum, rhipicephalus lunulatus, rhipicephalus ...200415139086
immunity against boophilus annulatus induced by the bm86 (tick-gard) vaccine.friesian cattle were immunized with two inoculations of anti-tick bm86 (tick-gard) vaccine and were challenged 30 or 90 d later with boophilus annulatus larvae derived from 1.2 g of eggs. no nymphs or adult ticks were found on the immunized cattle during four weeks after challenge. repeated infestations (2 to 4) with larvae on three other calves during a period of 160 and 390 d after the immunization did not result in development of nymphal and adult stages. in control, non-immunized cattle infe ...200314580066
the susceptibility of different species and stages of ticks to entomopathogenic fungi.boophilus annulatus, hyalomma excavatum and rhipicephalus sanguineus ticks were shown to be susceptible to different entomopathogenic fungi under laboratory conditions. comparative results of bioassays using five different fungal species showed that some strains of metarhizium anisopliae are highly pathogenic against various tick stages tested. in contrast to to their activity against insects, fungi also affected tick eggs. all tested tick stages including those feeding on a host were found to b ...200214570142
phylogeny, evolution and historical zoogeography of ticks: a review of recent progress.there has been much progress in our understanding of the phylogeny and evolution of ticks, particularly hard ticks, in the past 5 years. indeed, a consensus about the phylogeny of the hard ticks has emerged. our current working hypothesis for the phylogeny of ticks is quite different to the working hypothesis of 5 years ago. so that the classification reflects our knowledge of ticks, several changes to the nomenclature of ticks are imminent. one subfamily, the hyalomminae, will probably be sunk, ...200214570116
a survey of ticks (acari: ixodidae) on dairy cattle on the island of menorca in spain.a survey of ticks affecting dairy cattle under extensive management on menorca island (off the spanish mediterranean coast) was performed through 1999-2000 on seven farms. the species collected were rhipicephalus bursa, r. turanicus, hyalomma m. marginatum, h. lusitanicum and boophilus annulatus. the most abundant species were r. bursa and h. m. marginatum, with peaks of 10.7 (in summer) and 7.5 (in spring) ticks/cow, respectively. r. turanicus was scarcely encountered, while b. annulatus and h. ...200112455879
introduced ticks and tick-borne diseases: the threat and approaches to eradication.exotic tick species and tick-borne diseases are serious threats to live-stock, companion animals, and wildlife in the united states. recurring introductions of exotic tick species into the united states are a significant indicator of the degree of risk. successful tick-eradication campaigns, such as the national program that eradicated boophilus annulatus and b. microplus from the united states, the cattle fever tick eradication program of the us department of agriculture's veterinary services t ...200212442574
the ivomec sr bolus for control of boophilus annulatus (acari: ixodidae) on cattle in south texas.when hereford heifers infested with boophilus annulatus (say) were treated with a single ivomec sr bolus, the concentration of ivermectin in the serum of the treated cattle reached a maximum of 8.8 +/- 0.9 ppb at 2 wk posttreatment. the single bolus treatment resulted in 84.4% control of standard engorging b. annulatus females on treated cattle over the 20-wk trial. although fewer engorged ticks were collected from the sentinel heifers exposed in the treated pasture than those in the control pas ...200111777074
factors affecting the virulence of entomopathogenic nematodes to engorged female boophilus annulatus ticks.the laboratory trials rank the virulence of entomopathogenic nematode strains (3 heterorhabditids and 6 steinernematids) to engorged female boophilus annulatus ticks according to 3 parameters of the infection process: the effect of exposure time on tick mortality, the quantity of nematodes that penetrate ticks, and the rate of tick mortality after the injection of 1, 2, or 3 nematodes. exposure of the ticks to heterorhabditid strains for 6 hr resulted in >80% mortality, but only 20 or 65% mortal ...200111534645
detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in sicily.a reverse line blot hybridisation (rlb) of 21 oligonucleotides with polymerase chain reaction (pcr) amplified regions of 16s rrna (ehrlichia/anaplasma group) or 18s rrna (babesia/theileria group) genes of haemoparasites detected theileria annulata, t. buffeli/orientalis, babesia bovis, b. bigemina, b. divergens, ehrlichia bovis, anaplasma marginale, a. centrale and unknown species within the rickettsia tribe.a very high prevalence of mixed infections was detected, which indicated that animals in ...200111511414
observations on the structure and histology of the female genital system of the cattle tick boophilus annulatus (say).female genital system of boophilus annulatus was examined grossly on the day of drop-off from the host and grossly and microscopically on 4th day. it consists of an ovary, 2 oviducts, a common oviduct, a connecting tube, a seminal receptacle, a vagina divided into cervical and vestibular parts, 2 accessory glands and gene's organ. the ovary oocytes undergo excessive growth during the 4 days after drop-off from the host. oocytes accumulate eosinophilic material during growth and undergo histologi ...200111478455
investigation of blood parasites in livestock infested with argasid and ixodid ticks in egypt.nineteen species and subspecies of ixodid and argasid ticks, hyalomma dromedarii, h. impeltatum, h. anatolicum excavatum, h. a. anatolicum, h. truncatum, h. m. marginatum, h. m. rufipes, h. m. turanicum, boophilus annulatus, rhipicephalus sanguineus, r. turanicus, r. guilhoni, r. camicasi, amblyomma lipidum, a. marmoreum, a. vareigatum, argas persicus, a. hermanii and a. arboreus were collected from different localities in five governorates, giza, sharkia, ismailia, el beheira and sinai. hyalomm ...200111478436
biocontrol of ticks by entomopathogenic nematodes. research update.entomopathogenic nematodes (epns) are lethal to ticks even though they do not use their normal propagation cycle within tick cadavers. the tick boophilus annulatus was found to be far more susceptible to epns than hyalomma excavatum, rhipicephalus bursa, or rhipicephalus sanguineus. ticks seem to be less susceptible to nematodes when feeding on a host. preimaginal tick stages were less susceptible to nematodes than adult ticks. the mortality rate of unfed females was highest, followed by unfed m ...200011193678
clinical and laboratory studies on theileriosis outbreaks in sicily, italy.a total of 153 blood samples were collected all over the sicilian island in 15 farms. 29.4% and 25.5% were pcr positive for theileria annulata and t. buffeli/orientalis respectively. 15.7% of cattle blood samples showed a mixed infection with both theileria species. each farm showed an infection rate ranging from 0% to 100%. hyalomma marginatum and boophilus annulatus were the most common ticks collected in april and november 1998 respectively. the aim of the present work was to investigate on h ...199911071547
general review of tick species present in portugal.at present, 24 species are known to occur in portugal: argas vespertilionis, ornithodoros maritimus and ornithodoros erraticus in argasidae; ixodes acuminatus, ixodes bivari, ixodes canisuga, ixodes frontalis, ixodes hexagonus, ixodes ricinus, ixodes simplex simplex, ixodes ventalloi, ixodes vespertilionis, dermacentor marginatus, dermacentor pictus', haemaphysalis hispanica, haemaphysalis inermis, haemaphysalis punctata, rhipicephalus bursa, rhipicephalus pusillus, rhipicephalus sanguineus, rhi ...199911071535
distribution and ecology of ticks (acari: ixodidae) infesting livestock in tunisia: an overview of eighth years field collections.ticks (ixodidae) play a significant role as vectors of pathogens of domestic animals in tunisia. the major losses caused by ticks are related to transmission of protozoan parasites. these include agents of tropical theileriosis and babesiosis in ruminants. since 1991, we conducted research studies on tick population of livestock in tunisia. this overview reports a synthesis on tick distribution, their biology and their role as vectors of pathogens in domestic animals, particularly cattle. during ...199911071534
mortality rate of adult ticks due to infection by entomopathogenic nematodes.the mortality of boophilus annulatus, hyalomma excavatum, rhipicephalus bursa, and rhipicephalus sanguineus adult ticks was recorded after their exposure in petri dishes to 5 entomopathogenic nematode strains. the strains used were dt and mexican of steinernema carpocapsae, hb hp88 of heterorhabditis bacteriophora, and is-3 and is-5 of heterorhabditis sp. the most rapid killing rates (lt50 and lt90) were observed for b. annulatus ticks (0.8-5.0 days). most unfed males died 0.3-2.8 days after the ...200010958439
report on ticks collected in the southeast and mid-west regions of brazil: analyzing the potential transmission of tick-borne pathogens to man.specimens of ticks were collected in 1993, 1996, 1997, and 1998, mostly from wild and domestic animals in the southeast and mid-west regions of brazil. nine species of amblyommidae were identified: anocentor nitens, amblyomma cajennense, amblyomma ovale, amblyomma fulvum, amblyomma striatum, amblyomma rotundatum, boophilus microplus, boophilus annulatus, and rhipicephalus sanguineus. the potential of these tick species as transmitters of pathogens to man was analyzed. a flaviviridade flavivirus ...199910881097
cloning, characterization, and expression of a 200-kilodalton diagnostic antigen of babesia bigemina.current serological tests for babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. one of the major drawbacks of these tests is that antigen quality can vary from batch to batch. since the quality of the antigen contributes to the sensitivity and specificity of serological tests, the use of standardized recombinant antigens should ensure consistency in assay quality. previously, a 200-kda merozoite antigen (p200) was identified as a candidate diagnostic antige ...200010834983
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