Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| novel nitrogen-fixing acetic acid bacteria, gluconacetobacter johannae sp. nov. and gluconacetobacter azotocaptans sp. nov., associated with coffee plants. | diazotrophic bacteria were isolated, in two different years, from the rhizosphere and rhizoplane of coffee (coffea arabica l.) plants cultivated in mexico; they were designated as type dor and type sad isolates. they showed characteristics of the family acetobacteraceae, having some features in common with gluconacetobacter (formerly acetobacter) diazotrophicus, the only known n2-fixing species of the acetic acid bacteria, but they differed from this species with regard to several characteristic ... | 2001 | 11491326 |
| further observations on the interaction between sugar cane and gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. | sugar cane (saccharum spp.) variety sp 70-1143 was inoculated with gluconacetobacter diazotrophicus strain pal5 (atcc 49037) in two experiments. in experiment 1 the bacteria were inoculated into a modified, low sucrose ms medium within which micropropagated plantlets were rooted. after 10 d there was extensive anatomical evidence of endophytic colonization by g. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. the identity ... | 2001 | 11413211 |
| comparison of benefit to sugarcane plant growth and 15n2 incorporation following inoculation of sterile plants with acetobacter diazotrophicus wild-type and nif- mutants strains. | the ability of the nitrogen-fixing bacterial endophyte acetobacter diazotrophicus strain pal5 to enhance the growth of sugarcane sp70-1143 was evaluated in the growth chamber, greenhouse, and field by comparing plants inoculated with wild-type and nif mutant mad3a in two independent experiments. the wild-type and nif mutant strains colonized sugarcane plants equally and persisted in mature plants. in n-deficient conditions, sugarcane plants inoculated with a. diazotrophicus pal5 generally grew b ... | 2001 | 11277433 |
| fructo-oligosaccharides production by the gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast pichia pastoris. | levansucrase (lsda) (ec 2.4.1.10) from gluconacetobacter diazotrophicus (formerly acetobacter diazotrophicus) yields high levels of fructo-oligosaccharides (fos) from sucrose. a dna fragment encoding the precursor lsda lacking the first 57 amino acids was fused to the pho1 signal sequence under the control of the pichia pastoris-alcohol oxidase 1 (aox1) promoter. methanol induction of a p. pastoris strain harboring a single copy of the lsda expression cassette integrated in the genome resulted i ... | 2001 | 11166804 |
| transfer of acetobacter oboediens sokollek et al 1998 and acetobacter intermedius boesch et al. 1998 to the genus gluconacetobacter as gluconacetobacter oboediens comb. nov. and gluconacetobacter intermedius comb. nov. | acetobacter oboediens sokollek et al. 1998 and acetobacter intermedius boesch et al. 1998 are transferred to the genus gluconacetobacter as gluconacetobacter oboediens comb. nov. and gluconacetobacter intermedius comb. nov. because, on the basis of their 16s rrna gene sequences, the type strains of both species are located in the cluster of the genus gluconacetobacter along with those of gluconacetobacter xylinus, gluconacetobacter europaeus, gluconacetobacter hansenii, gluconacetobacter liquefa ... | 2000 | 11155999 |
| characterization of a major cluster of nif, fix, and associated genes in a sugarcane endophyte, acetobacter diazotrophicus. | a major 30.5-kb cluster of nif and associated genes of acetobacter diazotrophicus (syn. gluconacetobacter diazotrophicus), a nitrogen-fixing endophyte of sugarcane, was sequenced and analyzed. this cluster represents the largest assembly of contiguous nif-fix and associated genes so far characterized in any diazotrophic bacterial species. northern blots and promoter sequence analysis indicated that the genes are organized into eight transcriptional units. the overall arrangement of genes is most ... | 2000 | 11092875 |
| the carbon source influences the energetic efficiency of the respiratory chain of n2-fixing acetobacter diazotrophicus. | acetobacter diazotrophicus is a diazotrophic bacterium that colonizes sugarcane tissues. glucose is oxidized to gluconate in the periplasm prior to uptake and metabolism. a membrane-bound glucose dehydrogenase quinoenzyme [which contains pyrroloquinoline quinone (pqq) as the prosthetic group] is involved in that oxidation. gluconate is oxidized further via the hexose monophosphate pathway and tricarboxylic acid cycle. a. diazotrophicus pal3 was grown in a chemostat with atmospheric nitrogen as t ... | 2000 | 11092633 |
| binding of soluble glycoproteins from sugarcane juice to cells of acetobacter diazotrophicus. | sugarcane produces two different pools of glycoproteins containing a heterofructan as glycidic moiety, tentatively defined as high-molecular mass (hmmg) and mid-molecular mass (mmmg) glycoproteins. both kinds of glycoproteins can be recovered in sugarcane juice. fluorescein-labelled glycoproteins are able to bind to acetobacter diazotrophicus cells, a natural endophyte of sugarcane. this property implies the aggregation of bacterial cells in liquid culture after addition of hmmg or mmmg. anionic ... | 2000 | 11032311 |
| cloning and sequencing of the levansucrase gene from acetobacter xylinum nci 1005. | the levansucrase gene (lsxa) was cloned from the genomic dna of acetobacter xylinum nci 1005, and the nucleotide sequence of the lsxa gene (1,293 bp) was determined. the deduced amino acid sequence of the lsxa gene showed 57.4% and 46.2% identity with the levansucrases from zymomonas mobilis and erwinia amylovora, respectively, while only 35.2% identity with that from acetobacter diazotrophicus. the gene product of lsxa (lsxa) that was overproduced in e. coli coded for a polypeptide of molecular ... | 2000 | 10997873 |
| structural levansucrase gene (lsda) constitutes a functional locus conserved in the species gluconacetobacter diazotrophicus. | levansucrase (ec 2.4.1.10) was identified as a constitutive exoenzyme in 14 gluconacetobacter diazotrophicus strains recovered from different host plants in diverse geographical regions. the enzyme, consisting of a single 60-kda polypeptide, hydrolysed sucrose to synthesise oligofructans and levan. sugar-cane-associated strains of the most abundant genotype (electrophoretic type 1) showed maximal values of levansucrase production. these values were three-fold higher than those of the isolates re ... | 2000 | 10985751 |
| azospirillum, a free-living nitrogen-fixing bacterium closely associated with grasses: genetic, biochemical and ecological aspects. | azospirillum represents the best characterized genus of plant growth-promoting rhizobacteria. other free-living diazotrophs repeatedly detected in association with plant roots, include acetobacter diazotrophicus, herbaspirillum seropedicae, azoarcus spp. and azotobacter. four aspects of the azospirillum-plant root interaction are highlighted: natural habitat, plant root interaction, nitrogen fixation and biosynthesis of plant growth hormones. each of these aspects is dealt with in a comparative ... | 2000 | 10978548 |
| functional bradyrhizobium japonicum nifa expression under a hybrid nptii-nifh promoter in e. coli and acetobacter diazotrophicus srt4. | a hybrid promoter consisting of the in tandem fusion of the tn5 nptii and the klebsiella pneumoniae nifh promoters was constructed to study the functionality of the nif genes transcriptional activator nifa from bradyrhizobium japonicum in two different host bacteria. beta-galactosidase experiments in e. coli revealed that the hybrid nptii-nifh promoter can behave as a constitutive or a nifa-inducible promoter depending on the aeration conditions. expression of the b. japonicum nifa from the hybr ... | 1998 | 10932742 |
| natural endophytic occurrence of acetobacter diazotrophicus in pineapple plants. | the presence of endophytic acetobacter diazotrophicus was tested for pineapple plants (ananas comosus [l.] merr.) grown in the field. diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. phenotypic tests permitted the selection of presumptive nitrogen-fixing a. diazotrophicus isolates. restriction fragment length polymorphisms (rflps) of small subunit (ssu) rdna using total dna digested with endonuclease sphi and with endo ... | 2000 | 10790517 |
| molecular detection of gluconacetobacter sacchari associated with the pink sugarcane mealybug saccharicoccus sacchari (cockerell) and the sugarcane leaf sheath microenvironment by fish and pcr. | molecular tools for the detection of the newly described acetic acid bacterium gluconacetobacter sacchari from the pink sugarcane mealybug, saccharicoccus sacchari cockerell (homiptera: pseudococcidae), and in the sugarcane leaf sheath microenvironment were developed. g. sacchari specific 16s rrna-targeted oligonucleotide primers were designed and used in pcr amplification of g. sacchari dna directly from mealybugs, and in a nested pcr to detect low numbers of the bacteria from sugarcane leaf sh ... | 2000 | 10620720 |
| the respiratory system and diazotrophic activity of acetobacter diazotrophicus pal5. | the characteristics of the respiratory system of acetobacter diazotrophicus pal5 were investigated. increasing aeration (from 0.5 to 4.0 liters of air min(-1) liter of medium(-1)) had a strong positive effect on growth and on the diazotrophic activity of cultures. cells obtained from well-aerated and diazotrophically active cultures possessed a highly active, membrane-bound electron transport system with dehydrogenases for nadh, glucose, and acetaldehyde as the main electron donors. ethanol, suc ... | 1999 | 10559164 |
| description of gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug. | a new species of the genus gluconacetobacter, for which the name gluconacetobacter sacchari sp. nov. is proposed, was isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug, saccharicoccus sacchari, found on sugar cane growing in queensland and northern new south wales, australia. the nearest phylogenetic relatives in the alpha-subclass of the proteobacteria are gluconacetobacter liquefaciens and gluconacetobacter diazotrophicus, which have 98.8-99.3% and 97.9-98.5% 1 ... | 1999 | 10555349 |
| identification, sequencing and structural analysis of a nifa-like gene of acetobacter diazotrophicus. | a recombinant plasmid, pad101, containing a dna fragment of acetobacter diazotrophicus strain pal5 was isolated by its ability to restore nif+ phenotype to a nifa- ntrc- double mutant of azotobacter vinelandii. hybridization with the nifa genes of azospirillum brasilense located the nifa gene more precisely to specific fragments of pad101. dna sequencing of appropriate subclones of pad101 revealed that the nifa gene was adjacent to the nifb gene in a. diazotrophicus, and the 5' end of the nifb g ... | 1999 | 10530336 |
| levansucrase from acetobacter diazotrophicus srt4 is secreted via periplasm by a signal-peptide-dependent pathway. | acetobacter diazotrophicus srt4 secretes a constitutive levansucrase (lsda) (ec 2.4.1.10) that is responsible for sucrose utilization. immunogold electron microscopical studies revealed that lsda accumulates in the periplasm before secretion. the periplasmic and extracellular forms of the enzyme were purified to homogeneity. both proteins exhibited similar physical and biochemical characteristics indicating that lsda adopts its final conformation in the periplasm. the n-terminal sequence of matu ... | 1999 | 10441728 |
| purification and enzymic properties of the fructosyltransferase of streptococcus salivarius atcc 25975. | the recombinant fructosyltransferase (ftf) of streptococcus salivarius was expressed in escherichia coli and purified to electrophoretic homogeneity after a combination of adsorption, ion-exchange and gel-filtration chromatography. the n-terminal signal sequence of the ftf was removed by e. coli at the same site as in its natural host. the purified ftf exhibited maximum activity at ph 6.0 and 37 degrees c, was activated by ca2+, but inhibited by the metal ions cu2+, zn2+, hg2+ and fe3+. the enzy ... | 1999 | 10393084 |
| phylogenetic identification of two major nitrogen-fixing bacteria associated with sugarcane. | acetobacter diazotrophicus and herbaspirillum seropedicae were identified by genetic methods based on 16s rrna sequences. a specific pcr method in combination with probing was developed for a. diazotrophicus. the pcr system includes four primers, of which the primers named ac (ctgtttcccgcaagggac) and di (gcgccccattgctgggtt) generated an 445 bp amplicon in all of the 11 a. diazotrophicus strains tested. the phylogenetic position of h. seropedicae was determined. h. seropedicae forms with oxalobac ... | 1998 | 9924818 |
| substitution of asp-309 by asn in the arg-asp-pro (rdp) motif of acetobacter diazotrophicus levansucrase affects sucrose hydrolysis, but not enzyme specificity. | beta-fructofuranosidases share a conserved aspartic acid-containing motif (arg-asp-pro; rdp) which is absent from alpha-glucopyranosidases. the role of asp-309 located in the rdp motif of levansucrase (ec 2.4.1.10) from acetobacter diazotrophicus srt4 was studied by site-directed mutagenesis. substitution of asp-309 by asn did not affect enzyme secretion. the kcat of the mutant levansucrase was reduced 75-fold, but its km was similar to that of the wild-type enzyme, indicating that asp-309 plays ... | 1999 | 9895294 |
| effect of high sugar concentration on nitrogenase activity of acetobacter diazotrophicus. | acetobacter diazotrophicus is a nitrogen-fixing bacterium that growth inside sugar cane plant tissue where the sucrose concentration is approximately 10%. the influence of high sugar content on nitrogenase was measured in the presence of oxygen and of nitrogen added in the form of ammonium and amino acids. in all parameters analyzed, 10% sucrose protected nitrogenase against inhibition by oxygen, ammonium, some amino acids, and also to some extent by salt stress. the oxygen concentration at whic ... | 1998 | 9871014 |
| life in grasses: diazotrophic endophytes. | n2-fixing bacteria such as azoarcus spp., herbaspirillum spp, and acetobacter diazotrophicus can infect the interior of gramineous plants without causing symptoms of plant disease but do not survive in soil. like phytopathogens, they can penetrate into central tissues and spread systemically. there is no evidence for an endosymbiosis in living plant cells; however, the bacteria are physiologically active in the plant apoplast. | 1998 | 9587190 |
| nucleotide sequence of the nifh gene coding for nitrogen reductase in the acetic acid bacterium acetobacter diazotrophicus. | the nifh gene sequence of the nitrogen-fixing bacterium acetobacter diazotrophicus was determined with the use of the polymerase chain reaction and universal degenerate oligonucleotide primers. the gene shows highest pair-wise similarity to the nifh gene of azospirillum brasilense. the phylogenetic relationships of the nifh gene sequences were compared with those inferred from 16s rrna gene sequences. knowledge of the sequence of the nifh gene contributes to the growing database of nifh gene seq ... | 1998 | 9489028 |
| coffea arabica l., a new host plant for acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria. | acetobacter diazotrophicus was isolated from coffee plant tissues and from rhizosphere soils. isolation frequencies ranged from 15 to 40% and were dependent on soil ph. attempts to isolate this bacterial species from coffee fruit, from inside vesicular-arbuscular mycorrhizal fungi spores, or from mealybugs (planococcus citri) associated with coffee plants were not successful. other acid-producing diazotrophic bacteria were recovered with frequencies of 20% from the coffee rhizosphere. these n2-f ... | 1997 | 9293018 |
| structure of the repeating oligosaccharide from the lipopolysaccharide of the nitrogen-fixing bacterium acetobacter diazotrophicus strain pal 5. | acetobacter diazotrophicus is an acid-tolerant nitrogen-fixing bacterium found in roots, rhizosphere, stems, and leaves of sugar cane (saccharum officinarum) cultivated in brazil. the o-polysaccharide from the lipopolysaccharide of the root isolate strain pal 5 has been determined by a combination of methylation analysis and two-dimensional high field nmr spectroscopy. the pentasaccharide repeat has the structure: [formula: see text] minor resonances in the nmr spectra are consistent with the pr ... | 1997 | 9098959 |
| molecular characterization of the levansucrase gene from the endophytic sugarcane bacterium acetobacter diazotrophicus srt4. | the acetobacter diazotrophicus srt4 gene encoding levansucrase (ec 2.4.1.10) (isda) was isolated from a genomic library. the nucleotide sequence of a 2.3 kb dna fragment sufficient for complementation of a levansucrase-deficient mutant (obtained by ems treatment) was determined. the isda gene (1751 bp) coded for a polypeptide of molecular mass 64.9 kda with an isoelectric point of 5.2. the n-terminal amino acid sequence of the extracellular levansucrase indicated the presence of a precursor prot ... | 1996 | 8704949 |
| differentiation of capsular polysaccharides from acetobacter diazotrophicus strains isolated from sugarcane. | capsular polysaccharides (cpss) from six representative strains of acetobacter diazotrophicus were isolated and fractionated by gel filtration and anion-exchange chromatography. purified cpss obtained in the non-adsorbed fraction of a deae-sephadex a-25 column were qualitatively and quantitatively analyzed for sugar composition. uronic acid and amino sugars were not detected in all purified cpss. basically the cpss of a. diazotrophicus are composed of rhamnose, mannose, galactose and glucose. th ... | 1995 | 7651237 |
| isolation and enzymic properties of levansucrase secreted by acetobacter diazotrophicus srt4, a bacterium associated with sugar cane. | acetobacter diazotrophicus, a nitrogen-fixing bacterium associated with sugar cane, secretes a levansucrase (sucrose-2,6-beta-d-fructan 6-beta-d-fructosyltransferase; ec 2.4.1.10). this enzyme is constitutively expressed and represents more than 70% of the total proteins secreted by strain srt4. the purified protein consists of a single 58 kda polypeptide with an isoelectric point of 5.5. its activity is optimal at ph 5.0. it catalyses transfructosylation from sucrose to a variety of acceptors i ... | 1995 | 7619044 |