Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [phylogenetic diversity of the culturable rare actinomycetes in marine sponge hymeniacidon perlevis by improved isolation media]. | based on the molecular diversity information, seven actinomycete-selective culture media and isolation conditions were modified to isolate and cultivate diverse rare actinomycetes from hymeniacidon perlevis. | 2009 | 19873748 |
| detection and hazard assessment of pathogenic microorganisms in medical wastes. | this study was undertaken to investigate the types and concentrations of microbial agents in various medical wastes as well as to characterize their survivals in these wastes at different temperatures for microbial risk assessment. medical wastes collected from 5 major hospitals in south korea were classified and stored at three different temperatures (-20, 6, and 30 degrees c). presence of various microorganisms such as pathogenic viruses and bacteria were investigated by both cultivation and b ... | 2009 | 19827491 |
| characterization of the caenorhabditis elegans udp-galactopyranose mutase homolog glf-1 reveals an essential role for galactofuranose metabolism in nematode surface coat synthesis. | galactofuranose (gal(f)), the furanoic form of d-galactose produced by udp-galactopyranose mutases (ugms), is present in surface glycans of some prokaryotes and lower eukaryotes. absence of the gal(f) biosynthetic pathway in vertebrates and its importance in several pathogens make ugms attractive drug targets. since the existence of gal(f) in nematodes has not been established, we investigated the role of the caenorhabditis elegans ugm homolog glf-1 in worm development. glf-1 mutants display sig ... | 2009 | 19751718 |
| novel n-acylhomoserine lactone-degrading bacteria isolated from the leaf surface of solanum tuberosum and their quorum-quenching properties. | we isolated and identified ahl-degrading bacteria from the leaf surface of solanum tuberosum. the 16 isolates inactivated both short- and long-chain ahls. two of these isolates, identified as microbacterium testaceum, showed putative ahl-lactonase activity. these two strains interrupted quorum-sensing dependent bacterial infection by plant pathogen pectobacterium carotovorum subsp. carotovorum. m. testaceum strains, which were isolated in this study, might be useful in the biocontrol of plant di ... | 2009 | 19734660 |
| microbacterium azadirachtae sp. nov., a plant-growth-promoting actinobacterium isolated from the rhizoplane of neem seedlings. | microbacterium strain ai-s262(t) was isolated from the rhizoplane of neem seedlings in the botanical garden of tamilnadu agricultural university, coimbatore, india, and subjected to phenotypic, chemotaxonomic and genetic characterization. cells of this strain were gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. strain ai-s262(t) contained mk-12 and mk-13 as the main respiratory quinones, anteiso-c(15 : 0), anteiso-c(17 : 0) ... | 2010 | 19734284 |
| isolation of identical nitrilase genes from multiple bacterial strains and real-time pcr detection of the genes from soils provides evidence of horizontal gene transfer. | bacterial enzymes capable of nitrile hydrolysis have significant industrial potential. microbacterium sp. aj115, rhodococcus erythropolis aj270 and aj300 were isolated from the same location in england and harbour identical nitrile hydratase/amidase gene clusters. strain aj270 has been well studied due to its nitrile hydratase and amidase activity. r. erythropolis itcbp was isolated from denmark and carries a very similar nitrile hydratase/amidase gene cluster. in this study, an identical nitril ... | 2009 | 19730817 |
| vertical distribution and anaerobic biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments in hong kong, south china. | the vertical distribution of polycyclic aromatic hydrocarbons (pahs) at different sediment depths, namely 0-2 cm, 2-4 cm, 4-6 cm, 6-10 cm, 10-15 cm and 15-20 cm, in one of the most contaminated mangrove swamps, ma wan, hong kong was investigated. it was the first time to study the intrinsic potential of deep sediment to biodegrade pahs under anaerobic conditions and the abundance of electron acceptors in sediment for anaerobic degradation. results showed that the total pahs concentrations (summa ... | 2009 | 19683792 |
| microbacterium agarici sp. nov., microbacterium humi sp. nov. and microbacterium pseudoresistens sp. nov., isolated from the base of the mushroom agaricus blazei. | three gram-positive, rod-shaped bacteria (strains cc-sbck-209( t), cc-12309(t) and cc-5209(t)) were isolated from the stalk of the edible mushroom agaricus blazei grown in the laboratory. 16s rrna gene sequence analysis indicated that all three isolates clearly belonged to the genus microbacterium. strains cc-sbck-209( t) and cc-12309(t) were most related closely to the type strain of microbacterium halotolerans (95.9 and 96.1 % 16s rrna gene sequence similarity, respectively). these two novel s ... | 2010 | 19661494 |
| microbacterium soli sp. nov., an alpha-glucosidase-producing bacterium isolated from soil of a ginseng field. | five gram-type-positive, aerobic, rod-shaped, non-motile strains of microbacterium (dcy 17(t), ms1, ms2, ms3 and ms4) were isolated from soil from a ginseng field in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, these strains were shown to be related to microbacterium esteraromaticum dsm 8609(t) (96.1 %), m. xylanilyticum dsm 16914(t) (96.0 %), m. aquimaris js54-2(t) (95.6 %), m. insulae ds-66(t) (95.5 %), m. ketosireducens ifo 14548(t) (95.5 %) and m. arabinogalactano ... | 2010 | 19654351 |
| mutation of asparagine 76 in the center of glutamine transporter snat3 modulates substrate-induced conductances and na+ binding. | the glutamine transporter slc38a3 (snat3) plays an important role in the release of glutamine from brain astrocytes and the uptake of glutamine into hepatocytes. it is related to the vesicular gaba (gamma-aminobutyric acid) transporter and the slc36 family of proton-amino acid cotransporters. the transporter carries out electroneutral na+-glutamine cotransport-h+ antiport. in addition, substrate-induced uncoupled cation currents are observed. mutation of asparagine 76 to glutamine or histidine i ... | 2009 | 19596860 |
| stereospecific degradation of phenylsuccinate by actinomycetes. | racemic phenylsuccinate was stereospecifically degraded by the actinomycetes ps9 and ps17 isolated from soil obtained from okinawa island, japan. strain ps9, identified as a citricoccus sp., preferentially degraded the r-form, while strain ps17, identified as a microbacterium sp., preferentially degraded the s-form of phenylsuccinate. analysis of the culture broths of these species with phenylsuccinate as the sole carbon source revealed that benzoic acid was produced as a metabolic intermediate. ... | 2009 | 19580991 |
| occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland. | the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ... | 2009 | 19572487 |
| microbacterium invictum sp. nov., isolated from homemade compost. | strain dc-200t was isolated from homemade compost produced from kitchen refuse and characterized using a polyphasic approach. the isolate was a gram-positive motile short rod, facultatively aerobic, catalase-positive and oxidase-negative, and was able to grow at 10-37 degrees c, ph 6.0-9.5 and with up to 5% of nacl. the peptidoglycan was of the type b1 alpha and the muramic acid residues were glycolylated. the major fatty acids were anteiso-c15:0 and anteiso-c17:0. the predominant respiratory me ... | 2009 | 19567581 |
| microbacterium insulae sp. nov., isolated from soil. | a gram-positive, non-motile, rod- or coccoid-shaped microbacterium-like bacterium, designated strain ds-66(t), was isolated from soil of dokdo, korea, and its exact taxonomic position was investigated by using a polyphasic approach. strain ds-66(t) grew optimally at 30 degrees c and ph 6.5-7.0 in the presence of 0.5-1.0 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain ds-66(t) belonged to the genus microbacterium. strain ds-66(t) had a peptidoglycan type ba ... | 2009 | 19542140 |
| characterization of microbacterium sp. f10a and its role in polycyclic aromatic hydrocarbon removal in low-temperature soil. | a polycyclic aromatic hydrocarbon degrading bacterium isolated from oil-polluted soil was identified as microbacterium sp. f10a based on 16s rdna gene sequence analysis. plant growth promoting characteristics of the strain, degradation rate of phenanthrene and pyrene, and cell surface hydrophobicity characteristics of the strain were further characterized. the strain was also evaluated for promoting the growth of wheat and phenanthrene and pyrene removal from soil artificially contaminated with ... | 2009 | 19483781 |
| assessment of the microbial diversity at the surface of livarot cheese using culture-dependent and independent approaches. | the microbial diversity of the surface of a commercial red-smear cheese, livarot cheese, sold on the retail market was studied using culture-dependent and independent approaches. forty yeasts and 40 bacteria from the cheese surface were collected, dereplicated using single-strand conformation polymorphism (sscp) analysis and identified using rrna gene sequencing for the culture-dependent approach. the culture-independent approach involved cloning and sequencing of the 16s rrna gene and sscp anal ... | 2009 | 19481828 |
| microbacterium binotii sp. nov., isolated from human blood. | two strains of non-spore-forming, rod-shaped, gram-positive bacteria, cip 101303(t) and cip 102116, were isolated from human blood in 1976 and 1977, respectively. these strains had chemotaxonomic markers that were consistent with classification in the genus microbacterium, i.e. mk-10, mk-11 and mk-12 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, galactose, mannose and rhamnose as the cell-wall sugars and ornithine as the diamino acid in the cell-wall pept ... | 2009 | 19406785 |
| sand aggregation by exopolysaccharide-producing microbacterium arborescens--agsb. | in the rhizosphere, exopolymers are also known to be useful to improve the moisture-holding capacity. the ability of the isolates from coastal sand dunes to produce exopolymers was determined. among which the isolate, showing very high production of exopolysaccharide (eps), microbacterium arborescens--agsb, a facultative alkalophile was further studied for exopolymer production. the isolate a gram-positive non-spore forming, slender rod, catalase positive, oxidase negative, showed growth in 12% ... | 2009 | 19340484 |
| impact of two bacterial biocontrol agents on bacterial and fungal culturable groups associated with the roots of field-grown maize. | to assess the impact of bacillus amyloliquefaciens and microbacterium oleovorans on bacterial and fungal groups associated to the roots of field-grown maize. | 2009 | 19292823 |
| lateral transfer of genes for hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) degradation. | recent studies demonstrated that degradation of the military explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by species of rhodococcus, gordonia, and williamsia is mediated by a novel cytochrome p450 with a fused flavodoxin reductase domain (xpla) in conjunction with a flavodoxin reductase (xplb). pulse field gel analysis was used to localize xpla to extrachromosomal elements in a rhodococcus sp. and distantly related microbacterium sp. strain ma1. comparison of rhodococcus rhodochrous 1 ... | 2009 | 19270122 |
| biological interactions to select biocontrol agents against toxigenic strains of aspergillus flavus and fusarium verticillioides from maize. | biological control represent an alternative to the use of pesticides in crop protection. a key to progress in biological control to protect maize against fusarium verticillioides and aspergillus flavus maize pathogens are, to select in vitro, the best agent to be applied in the field. the aim of this study was to examine the antagonistic activity of bacterial and yeast isolates against f.verticillioides and a. flavus toxigenic strains. the first study showed the impact of bacillus amyloliquefaci ... | 2009 | 19247799 |
| the c. elegans hox gene egl-5 is required for correct development of the hermaphrodite hindgut and for the response to rectal infection by microbacterium nematophilum. | members of the hox gene family encode transcription factors that specify positional identity along the anterior-posterior axis of nearly all metazoans. one among the caenorhabditis elegans hox genes is egl-5. a deletion allele of egl-5 was isolated in a screen for animals which fail to develop swollen tails when exposed to the bacterial pathogen microbacterium nematophilum. we show that compromised rectal development, which occurs as a result of loss of egl-5 function, results in a failure of re ... | 2009 | 19232338 |
| hydrolysis of fenamiphos and its toxic oxidation products by microbacterium sp. in pure culture and groundwater. | a bacterium with an exceptional ability to hydrolyse fenamiphos and its toxic oxidation products fenamiphos sulfoxide and fenamiphos sulfone, all possessing poc bond was isolated from soil. based on 16s rrna gene determination, this bacterium was putatively identified as microbacterium esteraromaticum. the phenols (fenamiphos phenol, sulfoxide phenol and sulfone phenol) formed during bacterial hydrolysis resisted further degradation in mineral salts medium and sterile groundwater, but were trans ... | 2009 | 19195880 |
| [application of biological aerated filter in treating biomass wastewater and its microbial population characteristics]. | biomass wastewater contains plenty of cellulose, hemicellulose, starch, saccharide, organic acid and protein. it has high cod value and leads to environment pollution and resource waste. biomass wastewater was treated in this investigate using a biological aerated filter (baf) on laboratory scale, and the effect of treatment in different hydraulic retention time (hrt) had been studied. the results show that the optimum hrt is 10 h. the value of cod and tn in effluent is 74.90 mg/l and 1.21 mg/l ... | 2008 | 19186816 |
| growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp. during ripening in a cheese model medium. | the growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp., some common but rarely mentioned cheese bacteria, were investigated in a cheese model deacidified by debaryomyces hansenii during the ripening process. our results show that these bacteria had distinct growth and cheese flavour production patterns during the ripening process. p. vulgaris had the greatest capacity to produce not only the widest variety but also the highest quantities of volatile ... | 2009 | 19083231 |
| microbacterium profundi sp. nov., isolated from deep-sea sediment of polymetallic nodule environments. | a gram-positive, aerobic, neutrophilic and rod-shaped bacterium, strain shh49(t), was isolated from a deep-sea sediment sample collected from the east pacific polymetallic nodule region. the strain was able to grow within a temperature range of 4-35 degrees c and tolerated up to 7.5 % (w/v) nacl. strain shh49(t) was characterized chemotaxonomically by having mk-12 and mk-13 as predominant isoprenoid quinones, anteiso-c(15 : 0), iso-c(15 : 0), iso-c(16 : 0) and anteiso-c(17 : 0) as major fatty ac ... | 2008 | 19060085 |
| agrococcus versicolor sp. nov., an actinobacterium associated with the phyllosphere of potato plants. | the taxonomic position of a group of actinobacterial strains isolated from the phyllosphere of potato plants was investigated by using a polyphasic approach. although the similarity values for their 16s rrna gene sequences suggested an intermediate position between microbacterium and agrococcus, the phylogenetic tree demonstrated a clear clustering of the representative strain, k 114/01(t), within the genus agrococcus. the presence of 2,4-diaminobutyric acid as the diagnostic diamino acid in the ... | 2008 | 19060068 |
| crystallization of the hydantoin transporter mhp1 from microbacterium liquefaciens. | the integral membrane protein mhp1 from microbacterium liquefaciens transports hydantoins and belongs to the nucleobase:cation symporter 1 family. mhp1 was successfully purified and crystallized. initial crystals were obtained using the hanging-drop vapour-diffusion method but diffracted poorly. optimization of the crystallization conditions resulted in the generation of orthorhombic crystals (space group p2(1)2(1)2(1), unit-cell parameters a = 79.7, b = 101.1, c = 113.8 a). a complete data set ... | 2008 | 19052379 |
| endoxanthanase, a novel beta-d-glucanase hydrolyzing backbone linkage of intact xanthan from newly isolated microbacterium sp. xt11. | a novel endoxanthanase was produced and isolated from the culture of microbacterium sp. xt11 growing on xanthan. optimum ph and temperature for the enzyme activity were 6.0 and 35-40 degrees c, respectively. the endoxanthanase cleaves the backbone endo-beta-1,4-glucosidic linkage of the xanthan molecule, which is specific to the intact xanthan. however, the lyase-treated xanthan or carboxymethyl cellulose could not be cleaved by endoxanthanase. | 2009 | 19050833 |
| viable ultramicrocells in drinking water. | to examine the diversity of cultivable 0.2 micron filtrate biofilm forming bacteria from drinking water systems. | 2009 | 19040704 |
| the acyltransferase gene bus-1 exhibits conserved and specific expression in nematode rectal cells and reveals pathogen-induced cell swelling. | susceptibility to the rectal pathogen microbacterium nematophilum provides a means of examining hindgut differentiation in c. elegans. mutants of bus-1 are resistant to infection with this pathogen. we show here that bus-1 encodes a predicted acyltransferase expressed in rectal epithelial cells (k, f, and u), suggesting its involvement in regional surface modification. bus-1 reporter genes were used to show spatial regulation by hindgut developmental control genes: egl-38, mab-9, and mab-23. a b ... | 2008 | 19035336 |
| plasmid transformation and expression of the firefly luciferase in microbacterium testaceum type and endophytic colonizing field strains. | microbacterium testaceum is a predominant endophytic bacterial species isolated from corn and sorghum in the midwestern united states. the development of genetic transfer systems for m. testaceum may enable its use for biocontrol and other applications. the type strain (ifo 12675) and field isolates (se017, se034, and ce648) were grown to mid-exponential phase, concentrated (1.0 x 1011 cfu x ml(-1)), electroporated (escherichia coli-clavibacter shuttle plasmid pdm302), and plated on tsa with 10 ... | 2008 | 18997853 |
| microbacterium kribbense sp. nov., isolated from soil. | a gram-positive, short, rod-shaped, cream-coloured actinobacterium, designated msl-04(t), was isolated from a soil sample collected from an agricultural field on bigeum island (republic of korea) and was subjected to polyphasic characterization to unravel its taxonomic position. the optimum growth temperature and ph were 28-30 degrees c and ph 7.2-7.4. chemotaxonomic characteristics such as b2beta-type peptidoglycan with glycolyl residues, mk-11 and mk-12 as the major menaquinones and a fatty ac ... | 2008 | 18984689 |
| isolation and characterization of biosurfactant-producing alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis. | biosurfactant-producing bacteria belonging to the genera alcanivorax, cobetia and halomonas were isolated from marine sediments with a history of hydrocarbon exposure (aristizábal and gravina peninsulas, argentina). two alcanivorax isolates were found to form naturally occurring consortia with strains closely related to pseudomonas putida and microbacterium esteraromaticum. alkane hydroxylase gene analysis in these two alcanivorax strains resulted in the identification of two novel alkb genes, s ... | 2009 | 18983915 |
| interactions between strains of 2,4-diacetylphloroglucinol-producing pseudomonas fluorescens in the rhizosphere of wheat. | abstract strains of fluorescent pseudomonas spp. that produce the antibiotic 2,4-diacetylphoroglucinol (2,4-dapg) are among the most effective rhizobacteria controlling diseases caused by soilborne pathogens. the genotypic diversity that exists among 2,4-dapg producers can be exploited to improve rhizosphere competence and biocontrol activity. knowing that d-genotype 2,4-dapg-producing strains are enriched in some take-all decline soils and that p. fluorescens q8r1-96, a representative d-genotyp ... | 2003 | 18943865 |
| characterization of the microbial community involved in the suppression of pythium aphanidermatum in cucumber grown on rockwool. | abstract the root pathogen pythium aphanidermatum induced lower levels of disease in cucumber (cucumis sativus) plants on unsterilized, re-used rockwool slabs than on heat-sterilized, re-used rockwool. several recolonization treatments of the sterilized rockwool enhanced the suppressiveness of the rockwool. microbial community structures in the different rockwool treatments were investigated by plate counts on selective media. disease suppressiveness in the different rockwool treatments showed t ... | 2005 | 18943014 |
| structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter. | the nucleobase-cation-symport-1 (ncs1) transporters are essential components of salvage pathways for nucleobases and related metabolites. here, we report the 2.85-angstrom resolution structure of the ncs1 benzyl-hydantoin transporter, mhp1, from microbacterium liquefaciens. mhp1 contains 12 transmembrane helices, 10 of which are arranged in two inverted repeats of five helices. the structures of the outward-facing open and substrate-bound occluded conformations were solved, showing how the outwa ... | 2008 | 18927357 |
| normal intestinal flora of wild nile crocodiles (crocodylus niloticus) in the okavango delta, botswana. | bacterial and fungal cultures were performed from cloacal swabs collected from 29 wild nile crocodiles, captured in the okavango delta, botswana. sixteen species of bacteria and 6 fungal species were cultured. individual crocodiles yielded 1-4 bacterial species, and 0-2 fungal species. the most commonly isolated bacteria were microbacterium, enterococcus faecalis, aeromonas hydrophila, and escherichia coli. no salmonellae were cultured. the most commonly occurring fungus was cladosporium. severa ... | 2008 | 18846850 |
| isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the southwest indian ocean ridge. | microorganisms play an important role in the geobiocycling of arsenic element. however, little is known about the bacteria involved in this process in oceanic environments. in this report, arsenite-resistant bacteria were detected in deep-sea sediments on the southwest indian ridge. from arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2-80 mm. phylogenetic analysis based on 16s rrna showed that they mainly belonged to proteobacteria and actinob ... | 2009 | 18841325 |
| [diversity of actinomycetes in wuling mountain]. | to isolate new actinomycetes for discovering compounds of pharmaceutical importance. | 2008 | 18837376 |
| chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxb) in rhizobacteria isolated from crop plants. | twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16s rrna analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the beta and gamma subclasses of proteobacteria and actinobacteria. among these, five genera, including dyella, burkholderia, alcaligenes, microbacterium and leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. the thiosulfate-oxidizing dyella, burkholderia, alcaligenes, microbacterium, lei ... | 2008 | 18832027 |
| characterisation of aerobically grown non-spore-forming bacteria from paper mill pulps containing recycled fibres. | a total of 179 non-spore-forming bacteria aerobically growing on nutrient agar, plate count agar or in specific enrichment conditions for salmonella, campylobacteria, listeria, yersinia or staphylococci, were isolated from 16 untreated paper mill pulps. after phenotypical screening the isolates were characterised by automated ribotyping and partial sequencing of the 16s rrna gene. they could be divided into seven taxonomical classes representing 63 taxa (species): actinobacteria (11 species), ba ... | 2009 | 18820960 |
| application of compochip microarray to investigate the bacterial communities of different composts. | a microarray spotted with 369 different 16s rrna gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. the microarray was tested with pure cultures, and of the 30,258 individual probe-target hybridization reactions performed, there were only 188 false positive (0.62%) and 22 false negative signals (0.07%). labeled target dna was prepared by polymerase chain reaction amplification of 16s rrna genes using a cy5-labeled universa ... | 2009 | 18818861 |
| enhanced removal of dimethyl sulfide from a synthetic waste gas stream using a bioreactor inoculated with microbacterium sp. ntut26 and pseudomonas putida. | the removal of dimethyl sulfide (dms) from industrial gas streams has received a high priority due to its very low odorous threshold value and relatively low biodegradability compared to other reduced sulfur compounds. a variety of bacteria that utilize dms as a carbon/energy source have been studied and the degradation pathway elucidated. however, to date, there have been few reports on the industrial application of such bacteria inoculated into a bioreactor for dms treatment. an additional pro ... | 2009 | 18815822 |
| identities of microbacterium spp. encountered in human clinical specimens. | in the present study, 50 strains of yellow-pigmented gram-positive rods that had been isolated from human clinical specimens and collected over a 5-year period were further characterized by phenotypic and molecular genetic methods. all 50 strains belonged to the genus microbacterium, and together they represented 18 different species. microbacterium oxydans (n = 11), m. paraoxydans (n = 9), and m. foliorum (n = 7) represented more than half of the strains included in the present study. the isola ... | 2008 | 18799696 |
| conversion of puerarin into its 7-o-glycoside derivatives by microbacterium oxydans (cgmcc 1788) to improve its water solubility and pharmacokinetic properties. | microbacterium oxydans strain nj 6 isolated from soil samples converted puerarin into two novel compounds, puerarin-7-o-glucoside and puerarin-7-o-isomaltoside, via an unreported o-glycosylation of the phenolic hydroxyl group at the 7-position of puerarin. sucrose, maltotriose, and maltose could be used as glucosyl donors for glycosylation of puerarin, but uridine-diphosphate glucose, glucose, fructose, lactose, cyclodextrin, and starch could not. regardless of the position of b-ring in the (iso ... | 2008 | 18795283 |
| medium optimization for the production of cyclic adenosine 3',5'-monophosphate by microbacterium sp. no. 205 using response surface methodology. | response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3',5'-monophosphate (camp) with microbacterium sp. no. 205. a fractional factorial design (2(11-7)) was applied to evaluate the effects of different components in the medium. k(2)hpo(4), mgso(4) and naf were found to significantly influence on the camp production. the steepest ascent method was used to access the optimal region of the medium composition. the concentrations of the three ... | 2009 | 18778935 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading: brits culture (south africa). | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. cr(vi) reduction rate up to 8 times higher than the rate in previous cultures was achieved by the brits culture under aerobic conditions. near complete cr(vi) reduction was observed in batches under initial concentrations up to 200mg cr(vi)/l after incubati ... | 2008 | 18760438 |
| coevolution between attine ants and actinomycete bacteria: a reevaluation. | we reassess the coevolution between actinomycete bacteria and fungus-gardening (attine) ants. actinomycete bacteria are of special interest because they are metabolic mutualists of diverse organisms (e.g., in nitrogen-fixation or antibiotic production) and because pseudonocardia actinomycetes are thought to serve disease-suppressing functions in attine gardens. phylogenetic information from culture-dependent and culture-independent microbial surveys reveals (1) close affinities between free-livi ... | 2008 | 18752608 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading. | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. complete cr(vi) reduction in aerobic cultures was achieved at a high concentration of 200 mg/l after incubation for only 65 hours. under anaerobic conditions up to 150 mg, cr(vi)/l was completely removed after incubating for 130 to 155 hours, still higher t ... | 2008 | 18701792 |
| culture-independent nested pcr method reveals high diversity of actinobacteria associated with the marine sponges hymeniacidon perleve and sponge sp. | a culture-independent nested polymerase chain reaction (pcr) technique was used to investigate the diversity of actinobacteria communities associated with the sponges hymeniacidon perleve and sponge sp. the phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16s rrna sequencing of cloned dna fragments. a total of 196 positive clones were screened by restriction fragment length polymorphism (rflp) analysis; 48 unique operational taxonomic units (otus) were selected for ... | 2008 | 18670903 |
| identities of arthrobacter spp. and arthrobacter-like bacteria encountered in human clinical specimens. | after the initial description of arthrobacter spp. isolated from clinical specimens in the mid-1990s, very few further reports on arthrobacter spp. have appeared in the clinical microbiology literature. the aim of the present study was to elucidate the distribution of arthrobacter spp. and arthrobacter-like bacteria encountered in clinical specimens by studying 50 consecutively isolated or received strains of large-colony-forming, whiteish-grayish, non-cheese-like-smelling, nonfermentative gram- ... | 2008 | 18650355 |
| treatment of bromoamine acid wastewater using combined process of micro-electrolysis and biological aerobic filter. | the wastewater originated from the production of bromoamine acid was treated in a sequential system of micro-electrolysis (me) and biological aerobic filter (baf). decolorization and cod(cr) removal rate of the proposed system was investigated with full consideration of the influence of two major controlling factors such as organic loading rate (olr) and hydraulic retention time (hrt). the removal rate of cod(cr) was 81.2% and that of chrominance could be up to 96.6% at an olr of 0.56 kg m(-3)d( ... | 2009 | 18621476 |
| probing the critical residues for intramolecular fructosyl transfer reaction of a levan fructotransferase. | levan fructotransferase (lftase) preferentially catalyzes the transfructosylation reaction in addition to levan hydrolysis, whereas other levan-degrading enzymes hydrolyze levan into a levan-oligosaccharide and fructose. based on sequence comparisons and enzymatic properties, the fructosyl transfer activity of lftase is proposed to have evolved from levanase. in order to probe the residues that are critical to the intramolecular fructosyl transfer reaction of the microbacterium sp. al-210 lftase ... | 2008 | 18600048 |
| microbacterium luticocti sp. nov., isolated from sewage sludge compost. | strain sc-087b(t), isolated from sewage sludge compost during a study of bacterial diversity in composts, was characterized. the isolate was a gram-positive, short rod that was motile, catalase- and oxidase-negative and able to grow at 27-45 degrees c, ph 5.5-9.7 and in up to 10 % nacl. the peptidoglycan was of the b2beta type, containing the characteristic amino acids ornithine, homoserine and hydroxyglutamic acid. the muramic acid residues of the peptidoglycan were partially glycolylated. the ... | 2008 | 18599719 |
| microbacterium aquimaris sp. nov., isolated from seawater. | strains js54-2(t) and js63-1 were isolated from seawater at jeju, korea. cells of these strains were gram-positive, non-motile, non-spore-forming, short rods and formed yellow-pigmented colonies on tryptic soy agar. the strains had chemotaxonomic markers that were consistent with their classification in the genus microbacterium, i.e. mk-11, mk-12 and mk-10 as the major menaquinones, fatty acids that were predominantly iso- and anteiso-branched, galactose and glucose as the cell-wall sugars, pept ... | 2008 | 18599704 |
| characterization of heavy metal-resistant endophytic bacteria from rape (brassica napus) roots and their potential in promoting the growth and lead accumulation of rape. | two lead (pb)-resistant endophytic bacteria were isolated from rape roots grown in heavy metal-contaminated soils and characterized. a pot experiment was conducted for investigating the capability of the two isolates to promote the growth and pb uptake of rape from pb-amended soil. the two isolates were identified as pseudomonas fluorescens g10 and microbacterium sp. g16 based on the 16s rdna gene sequence analysis. strains g10 and g16 exhibited different multiple heavy metal and antibiotic resi ... | 2008 | 18490091 |
| identification of cis-regulatory elements from the c. elegans t-box gene mab-9 reveals a novel role for mab-9 in hypodermal function. | we have identified conserved non-coding elements (cnes) in the regulatory region of caenorhabditis elegans and caenorhabditis briggsae mab-9, a t-box gene known to be important for cell fate specification in the developing c. elegans hindgut. two adjacent cnes (a region 78 bp in length) are both necessary and sufficient to drive reporter gene expression in posterior hypodermal cells. the failure of a genomic mab-9::gfp construct lacking this region to express in posterior hypodermis correlates w ... | 2008 | 18402933 |
| the c. elegans glycosyltransferase bus-8 has two distinct and essential roles in epidermal morphogenesis. | ventral enclosure in caenorhabditis elegans involves migration of epidermal cells over a neuroblast substrate and subsequent adhesion at the ventral midline. organisation of the neuroblast layer by ephrins and their receptors is essential for this migration. we show that bus-8, which encodes a predicted glycosyltransferase, is essential for embryonic enclosure and acts in or with ephrin signalling to mediate neuroblast organisation and to permit epidermal migration. bus-8 acts non-cell-autonomou ... | 2008 | 18395708 |
| chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor. | using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ... | 2008 | 18392868 |
| genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants. | the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ... | 2008 | 18383223 |
| phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off shimokita peninsula. | "a meta-enzyme approach" is proposed as an ecological enzymatic method to explore the potential functions of microbial communities in extreme environments such as the deep marine subsurface. we evaluated a variety of extra-cellular enzyme activities of sediment slurries and isolates from a deep subseafloor sediment core. using the new deep-sea drilling vessel "chikyu", we obtained 365 m of core sediments that contained approximately 2% organic matter and considerable amounts of methane from offs ... | 2008 | 18368287 |
| characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients. | over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ... | 2008 | 18335166 |
| microbacterium hatanonis sp. nov., isolated as a contaminant of hairspray. | an aerobic, rod-shaped, gram-positive, oxidase-negative, catalase-positive bacterial isolate, strain fcc-01(t), originating as a contaminant of hairspray was characterized using phenotypic and molecular taxonomic methods. a 16s rrna gene sequence analysis revealed that the isolate belonged to the genus microbacterium and represented an evolutionary lineage that was distinct from recognized microbacterium species. cell-wall hydrolysate from the isolate contained ornithine and the cell-wall sugars ... | 2008 | 18319473 |
| elucidation of 2-hydroxybiphenyl effect on dibenzothiophene desulfurization by microbacterium sp. strain zd-m2. | the effect of 2-hydroxybiphenyl (2-hbp), the end product of dibenzothiophene (dbt) desulfurization via 4s pathway, on cell growth and desulfurization activity was investigated by microbacterium sp. the experimental results indicate that 2-hbp would inhibit the desulfurization activity. providing 2-hbp was added in the reaction media, the dbt degradation rate decreased along with the increase of 2-hbp addition. by contrast, cell growth would be promoted in the addition of 2-hbp at a low concentra ... | 2008 | 18296046 |
| assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches. | microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ... | 2008 | 18280146 |
| virulence of plant pathogenic bacteria attenuated by degradation of fatty acid cell-to-cell signaling factors. | diffusible signal factor (dsf) is a fatty acid signal molecule involved in regulation of virulence in several xanthomonas species as well as xylella fastidiosa. in this study, we identified a variety of bacteria that could disrupt dsf-mediated induction of virulence factors in xanthomonas campestris pv. campestris. while many bacteria had the ability to degrade dsf, several bacterial strains belonging to genera bacillus, paenibacillus, microbacterium, staphylococcus, and pseudomonas were identif ... | 2008 | 18257682 |
| microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field. | strain gsoil 259(t), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain gsoil 259(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens ifo 3750(t) (98.5 %) and microbacterium imperiale ifo 1261 ... | 2008 | 18218944 |
| a novel recombinant system for functional expression of myonecrotic snake phospholipase a(2) in escherichia coli using a new fusion affinity tag. | a novel recombinant expression system in escherichia coli was developed using conger eel galectin, namely, congerin ii, as an affinity tag. this system was applied for the functional expression of myotoxic lysine-49-phospholipase a(2) ([lys(49)]pla(2)), termed bpii and obtained from protobothrops flavoviridis (pf) venom. recombinant pf bpii fused with a congerin tag has been successfully expressed as a soluble fraction and showed better quantitative yield when folded correctly. the solubility of ... | 2008 | 18207418 |
| infections caused by coryneform bacteria in pediatric oncology patients. | invasive infections caused by coryneform bacteria are uncommon but have been reported with increasing frequency in recent decades, especially in immunocompromised persons. because pediatric experience is limited, we examined the epidemiology and clinical characteristics of these infections in children undergoing cancer therapy. | 2008 | 18174873 |
| the growth of microbacterium lacticum in a chemically defined medium. | 1949 | 18108881 | |
| effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation. | spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ... | 2008 | 18083857 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| sugar beet-associated bacterial and fungal communities show a high indigenous antagonistic potential against plant pathogens. | the aim of this study was to analyze microbial communities in/on sugar beet with special focus on antagonists toward plant pathogens. for this purpose, the composition of microorganisms isolated from the rhizosphere, phyllosphere, endorhiza, and endosphere of field-grown sugar beet plants was analyzed by a multiphasic approach at three different plant development stages at six locations in europe. the analysis of microbial communities by single strand conformation polymorphism (sscp) of 16s/18s ... | 2008 | 18060449 |
| degradation of phenanthrene on plant leaves by phyllosphere bacteria. | the activity of phyllosphere bacteria in the degradation of phenanthrene was investigated as a mechanism for the removal of atmospheric phenanthrene after its deposition on plant leaves. initially, leaf samples of six plant species were collected from two roadsides in bangkok to determine the presence of phenanthrene-degrading bacteria. the numbers of phenanthrene-degrading phyllosphere bacteria were varied and ranged from 3.5 x 10(4) to 1.95 x 10(7) cfu/g, in which the highest number was found ... | 2007 | 18057816 |
| interactions with microbial pathogens. | a wide variety of bacterial pathogens, as well as several fungi, kill c. elegans or produce non-lethal disease symptoms. this allows the nematode to be used as a simple, tractable model host for infectious disease. human pathogens that affect c. elegans include gram-negative bacteria of genera burkholderia, pseudomonas, salmonella, serratia and yersinia; gram-positive bacteria enterococcus, staphylococcus and streptococcus; and the fungus cryptococcus neoformans. microbes that are not pathogenic ... | 2005 | 18050390 |
| rheological characterization of levan polysaccharides from microbacterium laevaniformans. | levan polysaccharides were produced from microbacterium laevaniformans and its rheological behaviors were characterized as a function of concentration and temperature. the intrinsic viscosity of the purified levan was determined to be 0.38dl/g at 25 degrees c which was relatively higher than that of levans from other microbial sources. the flow behaviors of the levan solutions were characterized by the increase in the shear stress, giving more increments in the shear rate. thus, the levan soluti ... | 2008 | 17931701 |
| growth characteristics of brevibacterium, corynebacterium, microbacterium, and staphylococcus spp. isolated from surface-ripened cheese. | the growth characteristics of five bacteria, brevibacterium aurantiacum 1-16-58, corynebacterium casei dpc 5298(t), corynebacterium variabile dpc 5310, microbacterium gubbeenense dpc 5286(t), and staphylococcus saprophyticus 4e61, all of which were isolated from the surface of smear cheese, were studied in complex and chemically defined media. all of the coryneforms, except m. gubbeenense, grew in 12% salt, while b. aurantiacum and s. saprophyticus grew in 15% salt. all five bacteria assimilated ... | 2007 | 17921266 |
| microbacterium sediminicola sp. nov. and microbacterium marinilacus sp. nov., isolated from marine environments. | two novel microbacterium species are described on the basis of phenotypic, chemotaxonomic and genotypic studies. the two strains, designated ym10-847(t) and ym11-607(t), were isolated from river sediment and unidentified hydroid, respectively, of a marine lake. the strains were gram-positive, catalase-positive bacteria with l-ornithine as the diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-glycolyl. the major menaquinones were mk-10 and mk-11 for ym10-847(t ... | 2007 | 17911310 |
| diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates. | bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ... | 2007 | 17905875 |
| culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. | during cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. the diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms were investigated over a 10-month period. about 20% of the isolates were found to be novel species, indicating that there is still much to be learned about culturable psychrotrophs in ... | 2007 | 17890340 |
| adsorption of microorganisms onto an artificial siderophore-modified au substrate. | the hydroxamate-type artificial siderophore, tris[2-{3-(n-acetyl-n-hydroxamino)propylamido}propyl]aminomethane (tappa) and its fe(iii) complex, fe(iii)-tappa were prepared and characterized by several spectroscopic methods. fe(iii)-tappa exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, microbacterium flavescens, suggesting that fe(iii)-tappa can permeate the cell membrane of the microorganism. the adsorption of the fe(iii)-siderophore complex onto a de ... | 2007 | 17890077 |
| performance and microbial diversity of a membrane bioreactor treating real textile dyeing wastewater. | the textile industry is one of the major industries of taiwan but unfortunately it produces toxic and low biodegradable wastewater. to remedy this problem, this study compared the performance of the membrane bioreactor (mbr) and sequencing batch reactor (sbr) processes for treating real textile dyeing wastewater. the microbial diversity of the mbr process was also identified by a combination of culturing methods and molecular biotechnology. the removal efficiencies of the mbr process for color, ... | 2007 | 17879852 |
| isolation, characterization and complete nucleotide sequence of a novel temperate bacteriophage min1, isolated from the nematode pathogen microbacterium nematophilum. | we report the discovery, properties and complete sequence (46,365bp) of min1, the first bacteriophage to be reported for the coryneform genus microbacterium. this temperate phage is normally integrated into a stable plasmid, pmn1, found in cells of microbacterium nematophilum, a pathogen of certain soil nematodes including caenorhabditis elegans, but it can also grow lytically. the phage is lambdoid in morphology and in sequence, belonging to the family siphoviridae. general and specific feature ... | 2007 | 17869067 |
| diversity of microorganisms in decaying maize stalks revealed by a molecular method. | microbial diversity in decaying maize stalk was characterized by constructing and analyzing rrna gene clone library. total 47 otus were obtained from 82 bacterial clones, including proteobacteria (64.6%), actinobacteria (30.5%), bacteroidetes (2.4%) and firmicutes (2.4%). most proteobacterial clones were members of rhizobium, pseudomonas and stenotrophomonas. eighty-four percent of actinobacteria was related to microbacterium. only 14 otus were identified from 124 fungal clones, including ascomy ... | 2007 | 17846593 |
| vitamin requirements of microbacterium lacticum orla-jensen. | 1947 | 17797253 | |
| conversion of major ginsenoside rb1 to 20(s)-ginsenoside rg3 by microbacterium sp. gs514. | ginseng saponin, the most important secondary metabolite in ginseng, has various pharmacological activities. many studies have been directed towards converting major ginsenosides to the more active minor ginsenoside, rg3. due to the difficulty in preparing ginsenoside rg3 enzymatically, the compound has been mainly produced by either acid treatment or heating. a microbial strain gs514 was isolated from soil around ginseng roots in a field and used for enzymatic preparation of the ginsenoside rg3 ... | 2008 | 17764709 |
| characterization of predominant bacteria isolates from clean rooms in a pharmaceutical production unit. | to screen for the predominant bacteria strains distributed in clean rooms and to analyze their phylogenetic relationships. | 2007 | 17726748 |
| alkaliphilic and halophilic hydrocarbon-utilizing bacteria from kuwaiti coasts of the arabian gulf. | green animate materials from the intertidal zone of the arabian gulf coast accommodated more alkaliphilic and halophilic bacteria than inanimate materials. the alkaliphilic oil-utilizing bacteria, as identified by their 16s ribonucleic acid sequences, belonged to the following genera arranged in decreasing frequences: marinobacter, micrococcus, dietzia, bacillus, oceanobacillus, and citricoccus. the halophilic oil-utilizing bacteria belonged to the genera: marinobacter, georgenia, microbacterium ... | 2007 | 17710391 |
| production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses. | twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ... | 2007 | 17701035 |
| orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxin. | germination of orchid seeds is a complex process. in this paper we focus on interactions between the host-plant and its bacterial partners via indole-3-acetic acid (iaa). originally isolated from the roots of the epiphytic orchid dendrobium moschatum, the strains of rhizobium, microbacterium, sphingomonas, and mycobacterium genera were among the most active iaa producers. addition of exogenous tryptophan significantly enhanced auxin formation both in mineral and complex media. the presence of ia ... | 2007 | 17687544 |
| microbacterium indicum sp. nov., isolated from a deep-sea sediment sample from the chagos trench, indian ocean. | two bacterial strains, bbh6(t) and bbh9, were isolated from a deep-sea sediment sample collected from the chagos trench, indian ocean, at a depth of 5904 m. the two strains were closely related in their 16s rrna gene sequences (99.7 %), belonged to one genomic species and were virtually identical at the phenotypic level. microbacterium barkeri dsm 20145(t) was the nearest phylogenetic neighbour to the new isolates, with 16s rrna gene sequence similarity levels of 97.2-97.4 %. the new isolates ex ... | 2007 | 17684264 |
| isolation of bacterial endophytes from germinated maize kernels. | the germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation of kernel-associated bacterial endophytes. bacterial strains identified by partial 16s rrna gene sequencing as pantoea sp., microbacterium sp., frigoribacterium sp., bacillus sp., paenibacillus sp., and sphingomonas sp. were isolated from kernels of 4 different maize cultivars. genus pantoea was associated with a specific maize cultivar. the kernels of this cultivar were ofte ... | 2007 | 17668041 |
| [isolation of phenol-degrading bacteria from natural soil and their phylogenetic analysis]. | five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. they were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. strain phd-2, phd-4 and phd-5 belonged to the genera of ralstonia, acinetobacter and microbacterium respectively; strain phd-1 and phd-3 were from the genus of pseudomonas. homology compari ... | 2007 | 17633645 |
| purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant brachybacterium sp. strain lb25. | a bacterium that secretes maltooligosaccharide-forming amylase in a medium containing 12.5% (vol/vol) dimethylsulfoxide (dmso) was isolated and identified as brachybacterium sp. strain lb25. the amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kda. the enzyme was stable at ph 7.0-8.5 and active at ph 6.0-7.5. the optimum temperature at ph 7.0 was 35 degrees c in the presence of 5 mm cacl(2). the enzyme hydrolyzed starch to produce maltotriose primari ... | 2007 | 17619813 |
| microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. | in this study we evaluated the capacity of a defined microbial consortium (five bacteria: mycobacterium fortuitum, bacillus cereus, microbacterium sp., gordonia polyisoprenivorans, microbacteriaceae bacterium, naphthalene-utilizing bacterium; and a fungus identified as fusarium oxysporum) isolated from a pahs contaminated landfarm site to degrade and mineralize different concentrations (0, 250, 500 and 1000 mg kg(-1)) of anthracene, phenanthrene and pyrene in soil. pahs degradation and mineraliz ... | 2008 | 17572084 |
| molecular cloning, expression and characterization of a chitosanase from microbacterium sp. | a gene encoding a chitosanase (mschito) was cloned from microbacterium sp. ou01. the orf consists of 801 bp which encoded a polypeptide of 266 amino acid residues. the deduced amino acid sequence shows 98% identity to that of the chitosanase reported in pseudomonas sp. a-01. in addition, the fusion protein containing mschito was expressed in e. coli and purified using ni-nta affinity chromatography. the purified rmschito protein degraded the chitosan (the degree of deacetylation of 99%) and prod ... | 2007 | 17563859 |
| effects of ultraviolet radiation on the gram-positive marine bacterium microbacterium maritypicum. | although extensive information is available on the effect ultraviolet (uv) radiation has on gram-negative marine bacteria, there is a scarcity of data concerning uv radiation and gram-positive marine bacteria. the focus of this paper is on microbacterium maritypicum, with the gram-negative vibrio natriegens being used as a standard of comparison. m. maritypicum exhibited growth over a nacl range of 0-1000 mm: , with optimum growth occurring between 0 and 400 mm: nacl. in contrast, v. natriegens ... | 2007 | 17551790 |
| gene sequence phylogenies of the family microbacteriaceae. | the type strains of 32 species of 13 genera of the family microbacteriaceae were analysed with respect to gene-coding phylogeny for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca), and polyphosphate kinase (ppk). the resulting gene trees were compared with the 16s rrna gene phylogeny of the same strains. the topology of neighbour-joining and maximum parsimony phylogenetic trees, based on nucleic-acid sequences and protein sequences of housekeeping genes, differ ... | 2007 | 17551787 |
| papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo. | fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (approximately 1 cm) of papaya (carica papaya l. 'surya') planted on murashige and skoog (ms)-based papaya culture medium (23/50 nos.) during the 2-4 week period following in vitro culturing. these isolates were ascribed to six gram-negative genera, namely pantoea (p. ananatis), enterobacter (e. cloacae), brevundimonas (b. aurantiaca), sphingomonas, methylobacterium (m. rhodesianum), and agrobacterium (a. tumefac ... | 2007 | 17538647 |