Publications

diversity in soil fungi from undisturbed and disturbed celtis tala and scutia buxifolia forests in the eastern buenos aires province (argentina).	the rhizospheric soil microfungi from a native forest (undisturbed and disturbed) were studied using soil dilution plate and soil washing methods. fungi were isolated using slightly acid and alkaline culture media. 54 taxa were isolated: 49 from undisturbed forest soil and 37 from disturbed forest soil. acremonium sp., aspergillus ustus, coemansia pectinata, doratomyces stemonitis, fusarium solani, f. oxysporum, gliocladium roseum, humicola fusco-atra, mortierella sp., penicillium lilacinum, tri ...	2002	12002400
production of a monoclonal antibody specific to the genus trichoderma and closely related fungi, and its use to detect trichoderma spp. in naturally infested composts.	studies of the interactions between hyperparasitic fungi and their hosts are severely hampered by the absence of methods that allow the unambiguous identification of individual genera in complex environments that contain mixed populations of fungi, such as soil or compost. this study details the development of a monoclonal antibody (mf2) that allows the detection and recovery of trichoderma spp. in naturally infested composts, and the visualization of hyperparasitic strains of trichoderma during ...	2002	11988501
a novel lactonohydrolase responsible for the detoxification of zearalenone: enzyme purification and gene cloning.	zearalenone (zen) is converted into a far less oestrogenic product by incubation with clonostachys rosea ifo 7063. an alkaline hydrolase responsible for the detoxification was purified to homogeneity from the fungus by a combination of salt precipitation and column chromatography methods. the purified enzyme was homodimeric with a subunit molecular mass of 30 kda and contained an intra-subunit disulphide bridge. on the basis of the internal peptide sequences of the purified protein, we cloned th ...	2002	11978180
comparative degradation of oomycete, ascomycete, and basidiomycete cell walls by mycoparasitic and biocontrol fungi.	fourteen fungi (primarily representing mycoparasitic and biocontrol fungi) were tested for their ability to grow on and degrade cell walls (cws) of an oomycete (pythium ultimum), ascomycete (fusarium equisetii), and basidiomycete (rhizoctonia solani), and their hydrolytic enzymes were characterized. protein was detected in the cultural medium of eleven of the test isolates, and these fungi significantly degraded cws over the 14-day duration of the experiment. in general, a greater level of cw de ...	2002	11888164
biotransformation of 4beta-hydroxyeudesmane-1,6-dione by gliocladium roseum and exserohilum halodes.	biotransformation of sesquiterpene 4beta-hydroxyeudesmane-1,6-dione by the filamentous fungi gliocladium roseum and exserohilum halodes was achieved. with exserohilum halodes, only one metabolite was obtained, as a result of the regio- and stereoselective reduction of the keto group at c-1, which is difficult to achieve by chemical means. five metabolites were produced with gliocladium roseum, three of which, the 7alpha-hydroxylated, the 7alpha,11- and the 1alpha,8alpha-dihydroxylated derivative ...	2001	11684186
scale-up studies on a defined medium process for pilot plant production of illicicolin by gliocladium roseum.	illicicolin was cultivated at the 600-l pilot scale for purposes of material generation and process development. the initial medium containing oat flour was difficult operationally as a result of excessive foaming during sterilization, so a new defined medium process (with either glucose or sucrose as the carbon source), developed at the 23-l scale, was scaled up and improved for pilot scale needs. pilot scale media development efforts focused on exploring the highest concentration of media (1.0 ...	2001	11312705
negative fungal chemotropism to toxic metals.	hyphal growth responses of geotrichum candidum, gliocladium roseum, humicola grisea and trichoderma viride to cu and cd were studied using a simple tessellated agar tile system. negative chemotropic behaviour of hyphae, which included curling and growth away from metal-containing domains, occurred in all species and with both metals. both toxic metal and sucrose concentrations in the medium modulated the magnitude of the negative chemotropic effects observed. in general, greater concentrations o ...	2000	11111025
identification of a universally primed-pcr-derived sequence-characterized amplified region marker for an antagonistic strain of clonostachys rosea and development of a strain-specific pcr detection assay.	we developed a pcr detection method that selectively recognizes a single biological control agent and demonstrated that universally primed pcr (up-pcr) can identify strain-specific markers. antagonistic strains of clonostachys rosea (syn. gliocladium roseum) were screened by up-pcr, and a strain-specific marker was identified for strain gr5. no significant sequence homology was found between this marker and any other sequences in the databases. southern blot analysis of the pcr product revealed ...	2000	11055920
structure elucidation of roselipins, inhibitors of diacylglycerol acyltransferase produced by gliocladium roseum kf-1040.	the structures of roselipins 1a, 1b, 2a and 2b were elucidated by spectroscopic studies including 1h-1h cosy, 13c-1h cosy, 13c-1h hmqc and 13c-1h hmbc nmr experiments, and degradation experiments. they have the common skeleton of 2,4,6,8,10,12,14,16,18-nonamethyl-5,9,13-trihydroxy-2e,6e, 10e-icosenoic acid modified with a d-mannose and a d-arabinitol. roselipin a and b groups were stereoisomers at the arabinitol moiety, which esterified the fatty acid from the different terminal hydroxy residue. ...	1999	10726930
roselipins, inhibitors of diacylglycerol acyltransferase, produced by gliocladium roseum kf-1040.	gliocladium roseum kf-1040, a marine isolate, was found to produce a series of new inhibitors of diacylglycerol acyltransferase (dgat). four active compounds, designated roselipins 1a, 1b, 2a and 2b, were isolated from the fermentation broth of the producing strain by solvent extraction, ods column chromatography and preparative hplc. the highest production of roselipins was observed when cultured in the medium containing natural sea water. roselipins inhibit dgat activity with ic50 values of 15 ...	1999	10580381
glisoprenins c, d and e, new inhibitors of appressorium formation in magnaporthe grisea, from cultures of gliocladium roseum. 2. structure determination.		1998	9544946
glisoprenins c, d and e, new inhibitors of appressorium formation in magnaporthe grisea, from cultures of gliocladium roseum. 1. production and biological activities.	glisoprenins c, d, and e, new glisoprenin derivatives, were isolated together with glisoprenin a from submerged cultures of the deuteromycete gliocladium roseum ha190-95. all glisoprenins inhibited appressorium formation in magnaporthe grisea on inductive (hydrophobic) surfaces. the compounds exhibited moderate cytotoxic, but no antifungal, antibacterial or phytotoxic activities.	1998	9544931
production of free amino acids by some earthworm-borne microorganisms.	production of free amino acids by some earthworm-borne microorganisms was investigated in three different synthetic media. among the fungi tried gliocladium roseum and heterocephallum aurantiacum; among bacteria screened bacillus macerans and b. mycoides; and among actinomycetes tested streptomyces rimosus, s. violans, s. antibiticus, s. corchorusii and s. atroolivaceus produced significant amount of free amino acids. no correlations could be observed between vegetative growth and free amino aci ...	1995	8972137
selective effects of two systemic fungicides on soil fungi.	bas 317 00f was not toxic to the total count of fungi after 2 days but was regularly significantly toxic at the three doses after 5, 20 and 40 days and toxic at the low and the high doses after 80 days. in the agar medium, it was toxic to the counts of total fungi. aspergillus, a. terreus, rhizopus oryzae and mucor racemosus at the high dose. only the mycelial growth of trichoderma viride which was significantly inhibited by the three doses when this fungicide was added to the liquid medium. pol ...	1982	6290890
studies on the biosynthesis of phenols in fungi. biosynthesis of 3,4-dimethoxy-6-methyltoluquinol and gliorosein in gliocladium roseum i.m.i. 93 065.	1. gliorosein was obtained in excellent yield (150mg./200ml. of raulin-thom medium) from surface cultures of gliocladium roseum. its nuclear-magnetic-resonance spectrum showed conclusively that it is 1,6-dihydro-3,4-dimethoxy-6-methyltoluquinone. 2. sodium [2-(14)c]acetate was incorporated into gliorosein and the related products (3.3% conversion). the specific activities of these substances increased in the order gliorosein, 3,4-dimethoxy-6-methyltoluquinol, the related quinhydrone and quinone, ...	1967	6067663
incorporation of 5-methylorcylaldehyde and methionine into the acetogenin (polyketide) gliorosein in gliocladium roseum i.m.i. 93065.	1. methyl-(14)c-labelled 1,3-dihydroxy-4,5-dimethylbenzene, 5-methylorcylaldehyde and 5-methylorsellinic acid were synthesized from orcinol and sodium [(14)c]cyanide and tested for activity as precursors of gliorosein. ring-(14)c-labelled orcylaldehyde was also prepared. 5[(14)c]-methylorcylaldehyde was incorporated into gliorosein (36% conversion); all the radioactivity was located in the c-methyl groups. 5-methylorsellinic acid was decarboxylated by gliocladium roseum and the resulting phenol ...	1968	5691382
a perfusion technique for the detection of fungal interreaction. 1. effect of gliocladium roseum on six cellulolytic fungi.		1970	5531234
microbial cleavage of zearalenone.	1. zearalenone, a fungal oestrogenic compound, was subjected to microbial transformation studies. preliminary screening with 150 fungal species showed that gliocladium roseum was capable of metabolizing zearalenone in 80-90% yields. 2. large-scale fermentation with g. roseum produced a 1:1 mixture of 1-(3,5-dihydroxyphenyl)-10'-hydroxy-1-undecen-6'-one and 1-(3,5-dihydroxyphenyl)-6'-hydroxy-1-undecen-10'-one. the compounds were isolated and purified at -20 degrees c, and identified using spectro ...	1988	2969647
monoclonal antibodies to gliocladium roseum, a potential biological control fungus of sap-staining fungi in wood.	immunological probes were developed to discriminate between a potential biological control fungus and sap-staining fungi present in wood. this paper describes the production of monoclonal antibodies to isolated cell wall fragments of the biological control fungus gliocladium roseum. two monoclonals, designated 6a5 and 3f12, were characterized. their specificity was assessed by elisa, by immunogold silver staining light microscopy, by immunogold electron microscopy, and by immunoblotting. monoclo ...	1992	1479354
distributional patterns of mesophilous and thermophilous microfungi in two bahamian soils.	this study focuses on the characteristics displayed by mesophilous and thermophilous microfungal populations occurring in two tropical monodominant plant communities, a cocos nucifera grove and a casuarina equisetifolia forest, that provide distinctly different edaphic conditions. the mesophilous population sampled at 25 degrees c by the dilution plate method and the thermophilous population that developed on soil plates incubated at 45 degrees c consisted of 1693 isolates representing 60 specie ...	1975	1214847
the influence of water-logging stage upon the paddy soil mycoflora in the ukraine.	the soil fungal flora of paddy soils from the southern districts of the ukraine was investigated. from 287 soil samples 170 species were isolated. they belonged to the following genera: oomycetes 1.2%, zygomycetes 8.8%, ascomycetes 5.8%, deuteromycetes 84.2%. certain fungi were found to be dominant forms, among them mortierella alpina peyron., penicillium funiculosum thom, p. rugulosum thom, p. chrysogenum thom, acremonium strictum w. gams, acr. breve w. gams, alternaria alternata (fr.) riessler ...	1978	726714