Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| nonsuppurative meningoencephalitis associated with aleutian mink disease parvovirus infection in ranch mink. | severe nonsuppurative meningoencephalitis associated with aleutian mink disease parvovirus (adv) infection was observed in adult ranch mink. brain lesions included severe, locally extensive to coalescing lymphoplasmacytic meningoencephalitis with accompanying gliosis, satellitosis, and mild extension of inflammation into the leptomeninges. adv was identified in mesenteric lymph node, spleen, brain, and liver of affected mink by polymerase chain reaction techniques. sequences of the adv isolate ( ... | 2000 | 10730948 |
| replication of aleutian mink disease parvovirus in mink lymph node histocultures. | aleutian mink disease parvovirus (adv), causes an immune disorder with a persistent infection of lymphoid organs in adult mink. we studied replication of adv in gel-supported histocultures prepared from adult mink mesenteric lymph node (mln). evidence of virus replication in the histocultures was first observed by indirect immunofluorescence 72 h after incubation with virus. cells resembling lymphocytes and macrophages contained both adv capsid (vp2) and nonstructural (ns1 and ns2) proteins, and ... | 2000 | 10644831 |
| unusual, high genetic diversity of aleutian mink disease virus. | the genetic diversity of aleutian mink disease virus (amdv) was examined. sequences obtained from 35 clinical samples were compared with five published sequences. an unusual, high genetic variability was revealed. three phylogenetic subgroups of amdv were identified, and the presence of more than one genotype at some farms was detected. | 1999 | 10565948 |
| molecular and structural basis of the evolution of parvovirus tropism. | parvoviruses have small genomes and, consequently, are highly dependent on their host for various functions in their reproduction. since these viruses generally use ubiquitous receptors, restrictions are usually intracellularly regulated. a lack of mitosis, and hence absence of enzymes required for dna replication, is a powerful block of virus infection. allotropic determinants have been identified for several parvoviruses: porcine parvovirus, canine parvovirus (cpv), feline parvovirus (feline p ... | 1999 | 10497831 |
| replication of aleutian mink disease parvovirus in vivo is influenced by residues in the vp2 protein. | aleutian mink disease parvovirus (adv) is the etiological agent of aleutian disease of mink. several adv isolates have been identified which vary in the severity of the disease they elicit. the isolate adv-utah replicates to high levels in mink, causing severe aleutian disease that results in death within 6 to 8 weeks, but does not replicate in crandell feline kidney (crfk) cells. in contrast, adv-g replicates in crfk cells but does not replicate in mink. the ability of the virus to replicate in ... | 1999 | 10482625 |
| three-dimensional structure of aleutian mink disease parvovirus: implications for disease pathogenicity. | the three-dimensional structure of expressed vp2 capsids of aleutian mink disease parvovirus strain g (advg-vp2) has been determined to 22 a resolution by cryo-electron microscopy and image reconstruction techniques. a structure-based sequence alignment of the vp2 capsid protein of canine parvovirus (cpv) provided a means to construct an atomic model of the advg-vp2 capsid. the advg-vp2 reconstruction reveals a capsid structure with a mean external radius of 128 a and several surface features si ... | 1999 | 10400786 |
| epitope mapping of aleutian mink disease parvovirus virion protein vp1 and 2. | six overlapping fragments of the aleutian mink disease parvovirus (amdv) virion protein vp1 and 2 (vp1/2) gene were inserted into the expression vector pmal-c2. four of the clones carried large overlapping fragments covering the entire vp1/2 gene. the remaining two clones covered specifically chosen regions within the vp1/2 gene. using a western blotting detection system, sera from amdv-infected mink were tested against the recombinant polypeptides. these studies showed reactions primarily direc ... | 1999 | 10219758 |
| identification of a cell surface protein from crandell feline kidney cells that specifically binds aleutian mink disease parvovirus. | aleutian mink disease parvovirus (adv) is the etiological agent of aleutian disease of mink. the acute disease caused by adv consists of permissive infection of alveolar type ii cells that results in interstitial pneumonitis. the permissive infection is experimentally modeled in vitro by infecting crandell feline kidney (crfk) cells with a tissue culture-adapted isolate of adv, adv-g. adv-g vp2 empty virions expressed in a recombinant baculovirus system were analyzed for the ability to bind to t ... | 1999 | 10196278 |
| construction of pathogenic molecular clones of aleutian mink disease parvovirus that replicate both in vivo and in vitro. | the adv-g isolate of aleutian mink disease parvovirus (adv) replicates permissively in crandell feline kidney (crfk) cells but is nonpathogenic for mink, whereas the highly pathogenic adv-utah isolate is nonviable in crfk cells. to assign control of host range in crfk cells and pathogenicity to specific regions of the adv genome, we constructed a full-length molecular clone chimeric between adv-g and adv-utah. if either the map unit (mu) 54-65 (clone g/u-5) or mu 65-88 (clone g/u-7) sections wer ... | 1998 | 9837793 |
| a comparison between permissive and restricted infections with aleutian mink disease parvovirus (adv): characterization of the viral protein composition at nuclear sites of virus replication. | we used three-color fluorescent labeling and confocal microscopy to compare the permissive and the antibody-mediated, restricted replication of aleutian mink disease parvovirus (adv). in both permissive (crfk cells) and restricted (k562 cells) situations, both adv non-structural proteins (ns1 and ns2) concentrated at focal sites in the nucleus, which also contained viral dna. bromodeoxyuridine labeling demonstrated that these sites also supported active adv single-strand dna synthesis, indicatin ... | 1998 | 9784064 |
| vaccination with aleutian mink disease parvovirus (amdv) capsid proteins enhances disease, while vaccination with the major non-structural amdv protein causes partial protection from disease. | vaccination studies were performed with partially purified recombinant amdv vp1/2 capsids as well as with the major amdv non-structural protein (ns1). all vaccine constructs induced an antibody response, but did not prevent infection upon challenge with amdv. the severity of aleutian disease (ad) was judged by the serum gammaglobulin level, the quantity of peripheral blood cd8 lymphocytes, antibody titers to vp1/2 and ns1 proteins and mink death rates. the vp1/2 vaccine constructs enhanced the d ... | 1998 | 9682374 |
| analyses of leucocytes in blood and lymphoid tissues from mink infected with aleutian mink disease parvovirus (amdv). | mink were infected with aleutian mink disease parvovirus (amdv) and sacrificed at monthly intervals after infection. during this time humoral immune responses and leucocyte numbers in blood, mesenteric lymph node, spleen and thymus were monitored. serum hypergammaglobulinaemia was observed together with elevated antibody responses to amdv ns1 and vp1/2 proteins. in blood, a highly significant increase in cd8+ lymphocytes was observed. however, (presumed)cd4+ cells defined as cd3+cd8- cells, and ... | 1998 | 9656422 |
| two parvoviruses that cause different diseases in mink have different transcription patterns: transcription analysis of mink enteritis virus and aleutian mink disease parvovirus in the same cell line. | the two parvoviruses of mink cause very different diseases. mink enteritis virus (mev) is associated with rapid, high-level viral replication and acute disease. in contrast, infection with aleutian mink disease parvovirus (adv) is associated with persistent, low-level viral replication and chronic severe immune dysregulation. in the present report, we have compared viral transcription in synchronized crfk cells infected with either mev or adv using a nonradioactive rnase protection assay. the ov ... | 1997 | 9188563 |
| aleutian mink disease parvovirus infection of k562 cells is antibody-dependent and is mediated via an fc(gamma)rii receptor. | aleutian mink disease parvovirus (adv) infects macrophages but infection is restricted. using the human macrophage cell line u937, we reported infection to be antibody-dependent [22,23]. recently, we learned that the u937 cells were contaminated with the human cell line, k562 [12]. to clarify which cell line actually supported adv infection, we studied the antibody-dependent enhancement (ade) of adv infection in pure lines of u937 and k562. after infection with adv complexed with mink anti-adv a ... | 1997 | 9125049 |
| s-phase-dependent cell cycle disturbances caused by aleutian mink disease parvovirus. | we examined replication of the autonomous parvovirus aleutian mink disease parvovirus (adv) in relation to cell cycle progression of permissive crandell feline kidney (crfk) cells. flow cytometric analysis showed that adv caused a composite, binary pattern of cell cycle arrest. adv-induced cell cycle arrest occurred exclusively in cells containing de novo-synthesized viral nonstructural (ns) proteins. production of adv ns proteins, indicative of adv replication, was triggered during s-phase trav ... | 1997 | 8995664 |
| expression of aleutian mink disease parvovirus capsid proteins in defined segments: localization of immunoreactive sites and neutralizing epitopes to specific regions. | the capsid proteins of the adv-g isolate of aleutian mink disease parvovirus (adv) were expressed in 10 nonoverlapping segments as fusions with maltose-binding protein in pmal-c2 (pvp1, pvp2a through pvp2i). the constructs were designed to capture the vp1 unique sequence and the portions analogous to the four variable surface loops of canine parvovirus (cpv) in individual fragments (pvp2b, pvp2d, pvp2e, and pvp2g, respectively). the panel of fusion proteins was immunoblotted with sera from mink ... | 1997 | 8985402 |
| investigation of an outbreak of aleutian disease on a commercial mink ranch. | to determine the modes of transmission of aleutian mink disease in a natural outbreak. | 1996 | 8950422 |
| a cluster of cases of juvenile mediastinal lymphoma in a ferret colony. | three cases of juvenile mediastinal lymphoma developed in a laboratory colony of ferrets. two ferrets became acutely moribund, and one was found dead with no preceding signs of illness. splenomegaly, hepatomegaly, and a large thoracic mass were the primary features in each case. all three ferrets had multiorgan metastasis of the tumor. two ferrets were tested for feline leukemia virus and aleutian disease virus with negative results. | 1996 | 8799931 |
| identification of a dna segment in ferret aleutian disease virus similar to a hypervariable capsid region of mink aleutian disease parvovirus. | a 401bp dna fragment of ferret aleutian disease virus (adv) was amplified using pcr primers spanning a hypervariable region of mink adv capsid sequence. the amplified fragment was 88-89% homologous to the same region of previously known sequence of three different strains of mink adv, however, as low as 54% homology was observed when compared with a 39bp segment known as hypervariable region. within the predicted 13 amino acid hypervariable region, the ferret adv sequence differed at 6 positions ... | 1996 | 8774691 |
| clusters of lymphoma in ferrets. | cluster outbreaks of lymphoma and leukemia have been associated with viral infections in many species including humans, cattle, and cats. this study describes epidemiological, clinical, and pathological features of cluster outbreaks of lymphoma in multiferret households and examines and compares the aleutian disease virus (adv) and feline leukemia virus (felv) status of cases, ferrets at risk, and controls. three ferret groups with 21 cases of histologically diagnosed lymphoma (12.6% cumulative ... | 1996 | 8630683 |
| subcellular localization of aleutian mink disease parvovirus proteins and dna during permissive infection of crandell feline kidney cells. | confocal microscopy allowed us to localize viral nonstructural (ns) and capsid (vp) proteins and dna simultaneously in cells permissively infected with aleutian mink disease parvovirus (adv). early after infection, ns proteins colocalized with viral dna to form intranuclear inclusions, whereas vp proteins formed hollow intranuclear shells around the inclusions. later, nuclei had irregular outlines and were virtually free of adv products. in these cells, inclusions of viral dna with or without as ... | 1996 | 8627805 |
| investigation of the pathogenesis of transplacental transmission of aleutian mink disease parvovirus in experimentally infected mink. | the transplacental transmission of aleutian mink disease parvovirus (adv) was studied in experimental infection of 1-year-old female non-aleutian mink. the adv-seronegative female mink were inoculated with adv prior to mating or after the expected implantation of the embryos during pregnancy. a group of uninfected females served as a control group. animals from each group were killed prior to or shortly after parturition. the in situ hybridization technique with radiolabeled strand-specific rna ... | 1996 | 8627663 |
| the relationship between capsid protein (vp2) sequence and pathogenicity of aleutian mink disease parvovirus (adv): a possible role for raccoons in the transmission of adv infections. | aleutian mink disease parvovirus (adv) dna was identified by pcr in samples from mink and raccoons on commercial ranches during an outbreak of aleutian disease (ad). comparison of dna sequences of the hypervariable portion of vp2, the major capsid protein of adv, indicated that both mink and raccoons were infected by a new isolate of adv, designated adv-tr. because the capsid proteins of other parvoviruses play a prominent role in the determination of viral pathogenicity and host range, we decid ... | 1996 | 8551624 |
| characterization of chimeric full-length molecular clones of aleutian mink disease parvovirus (adv): identification of a determinant governing replication of adv in cell culture. | the adv-g strain of aleutian mink disease parvovirus (adv) is nonpathogenic for mink but replicates permissively in cell culture, whereas the adv-utah 1 strain is highly pathogenic for mink but replicates poorly in cell culture. in order to relate these phenotypic differences to primary genomic features, we constructed a series of chimeric plasmids between a full-length replication-competent molecular clone of adv-g and subgenomic clones of adv-utah 1 representing map units (mu) 15 to 88. after ... | 1993 | 8396664 |
| aleutian mink disease parvovirus infection of mink peritoneal macrophages and human macrophage cell lines. | aleutian mink disease parvovirus (adv) mrnas are found in macrophages in lymph nodes and peritoneal exudate cells from adv-infected mink. therefore, we developed an in vitro infection system for adv by using primary cultures of mink macrophages or macrophage cell lines. in peritoneal macrophage cultures from adult mink, virulent adv-utah i strain showed nuclear expression of viral antigens with fluorescein isothiocyanate-labeled adv-infected mink serum, but delineation of specific viral proteins ... | 1993 | 8383229 |
| cis-acting sequences in the aleutian mink disease parvovirus late promoter important for transcription: comparison to the canine parvovirus and minute virus of mice. | we are currently investigating the regulation of transcription of the aleutian mink disease parvovirus (adv). adv causes a chronic immune complex-mediated condition known as classical aleutian disease, characterized by slow viral replication. this slow replication is an intrinsic property of adv and distinguishes it from the more prototypic parvoviruses such as minute virus of mice (mvm) and canine parvovirus (cpv). we have previously suggested a role for the weak adv promoters in the slow repli ... | 1993 | 8383216 |
| comparison of promoter activity in aleutian mink disease parvovirus, minute virus of mice, and canine parvovirus: possible role of weak promoters in the pathogenesis of aleutian mink disease parvovirus infection. | aleutian mink disease parvovirus (adv) infection causes both acute and chronic disease in mink, and we have previously shown that it is the level of viral gene expression that determines the disease pattern. to study the gene regulation of adv, we have cloned the p3 adv and p36 adv promoters in front of a reporter gene, the chloramphenicol acetyltransferase (cat) gene, and analyzed these constructs by transient transfection in a feline kidney cell line and mouse nih 3t3 cells. the genes for adv ... | 1993 | 8383215 |
| expression of aleutian mink disease parvovirus proteins in a baculovirus vector system. | we have previously published a detailed transcription map of aleutian mink disease parvovirus (adv) and proposed a model for the translation of the two virion structural proteins (vp1 and vp2) and three nonstructural proteins (ns-1, ns-2, and ns-3) (s. alexandersen, m. e. bloom, and s. perryman, j. virol. 62:3684-3994, 1988). to verify and further characterize this model, we cloned the predicted open reading frames for ns-1, ns-2, ns-3, vp1-vp2, and vp2 alone into a recombinant baculovirus and e ... | 1993 | 8380073 |
| pathogenesis of aleutian mink disease parvovirus infection: effects of suppression of antibody response on viral mrna levels and on development of acute disease. | we suppressed the b-cell development and antibody response in mink by using treatment with polyclonal anti-immunoglobulin m (anti-igm) to study the effects of antiviral antibodies on development of aleutian mink disease parvovirus (adv)-induced disease in more detail. newborn mink kits were injected intraperitoneally with 1 mg of either anti-igm or a control preparation three times a week for 30 to 34 days. at 21 days after birth, groups of mink kits were infected with the highly virulent united ... | 1994 | 8289377 |
| aleutian mink disease parvovirus infection of mink macrophages and human macrophage cell line u937: demonstration of antibody-dependent enhancement of infection. | aleutian mink disease parvovirus (adv) infects macrophages in adult mink. the virulent adv-utah i strain, but not the cell culture-adapted adv-g strain, infects mink peritoneal macrophage cultures and the human macrophage cell line u937 in vitro. however, preincubation of adv-g with adv-infected mink serum enhanced its infectivity for u937 cells. the enhancing activity was present in the protein a-binding immunoglobulin g fraction in the serum, but f(ab')2 fragments failed to enhance the infecti ... | 1993 | 8230426 |
| interstitial nephritis in aleutian mink disease. possible role of cell-mediated immunity against virus-infected tubular epithelial cells. | aleutian mink disease (ad) has been characterized by immune complex glomerulonephritis associated with persistent infection of aleutian mink disease parvovirus (adv). histopathological examination of kidneys from adv-infected mink in this study revealed that interstitial nephritis characterized by prominent damage of renal tubuli and lymphocyte infiltration was also common in ad along with glomerulonephritis. by using strand-specific in situ molecular hybridization technique, replication of adv ... | 1994 | 8203470 |
| acute interstitial pneumonia in mink kits inoculated with defined isolates of aleutian mink disease parvovirus. | the present study addressed the causal role of aleutian mink disease parvovirus (adv) in acute interstitial pneumonia in mink kits. all the examined isolates of adv caused interstitial pneumonia in newborn kits, although the severity of disease and the mortality varied. these findings indicate that adv is the direct causal agent of this disease in mink kits and that cofactors, which could have been present in the original adv-k isolate, do not play a role. acute interstitial pneumonia characteri ... | 1994 | 8203085 |
| role of alveolar type ii cells and of surfactant-associated protein c mrna levels in the pathogenesis of respiratory distress in mink kits infected with aleutian mink disease parvovirus. | neonatal mink kits infected with aleutian mink disease parvovirus (adv) develop an acute interstitial pneumonia with clinical symptoms and pathological lesions that resemble those seen in preterm human infants with respiratory distress syndrome and in human adults with adult respiratory distress syndrome. we have previously suggested that adv replicates in the alveolar type ii epithelial cells of the lung. by using double in situ hybridization, with the simultaneous use of a probe to detect adv ... | 1994 | 8139047 |
| nursing disease in mink: clinical and postmortem findings. | one hundred fifty lactating mink on one ranch in southern ontario were monitored over the lactation period in 1989 for evidence of clinical disease, and serial blood samples were collected for biochemical analysis. antemortem blood samples were collected and necropsies performed on the 17 females that developed nursing disease and on 17 controls matched to the same stage of lactation and on ten nonlactating controls. twenty-two additional nursing disease cases were selected from seven ranches in ... | 1993 | 8116144 |
| expression of aleutian mink disease parvovirus capsid proteins in a baculovirus expression system for potential diagnostic use. | a 2.3-kb cdna clone encoding aleutian mink disease parvovirus (adv) structural proteins vp1 and vp2 was inserted into the polyhedron gene of autographa californica nuclear polyhedrosis virus (acnpv) and expressed by the recombinant virus, acadv-1, in spodoptera frugiperda-9 cells. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western immunoblot analysis (wia) indicated that synthesis of both vp1 and vp2 was being directed by acadv-1. fluorescence microscopic examination of acadv- ... | 1994 | 8011776 |
| sequence comparison of the non-structural genes of four different types of aleutian mink disease parvovirus indicates an unusual degree of variability. | the present work shows that at least four different sequence types of aleutian mink disease parvovirus (adv) are present in adv isolates from mink. we here report the nucleotide sequences of these four types of adv from nucleotide 123 to 2208 (map unit 3 to 46). this part of the genome encodes three non-structural (ns) proteins of adv. comparison of the deduced amino acid sequences of these ns proteins showed that the adv proteins are much less conserved than the ns proteins from other members o ... | 1994 | 7998830 |
| aleutian mink disease: puzzles and paradigms. | aleutian mink disease (ad) is a naturally occurring persistent virus infection of mink caused by the aleutian mink disease parvovirus (adv). the classical form of ad, which occurs in adult mink, is notable for high titers of antiviral antibodies, hypergammaglobulinemia, plasmacytosis, and immune complex disease. in addition, there is a progressive renal disease characterized by mesangial proliferative glomerulonephritis and severe interstitial nephritis. development of ad depends on both host an ... | 1994 | 7889316 |
| purification and characterization of the major nonstructural protein (ns-1) of aleutian mink disease parvovirus. | we have previously described the expression of the major nonstructural protein (ns-1) of aleutian mink disease parvovirus (adv) in insect cells by using a baculovirus vector (j. christensen, t. storgaard, b. bloch, s. alexandersen, and b. aasted, j. virol. 67:229-238, 1993). to study its biochemical properties, adv ns-1 was expressed in sf9 insect cells and purified to apparent homogeneity with a combination of nuclear extraction, zn2+ ion chromatography, and immunoaffinity chromatography on mon ... | 1995 | 7853520 |
| ocular lesions in mink affected with aleutian disease. | uveitis, characterized by infiltrates of lymphocytes and plasma cells, was the principal ocular lesion in 122 sapphire and pastel mink affected with experimental aleutian disease. it was present to various degrees in all but five mink examined five to 164 weeks after inoculation (intraperitoneal or intranasal) with any of four north american strains of aleutian disease virus. the uveitis, mostly iridocyclitis, was accompanied often by protein-rich fluid in the anterior chamber and less often by ... | 1982 | 7200277 |
| inhibition of precipitation in counter current electrophoresis. a sensitive method for detection of mink antibodies to aleutian disease virus. | inhibition of precipitation in counter current electrophoresis was at least 32 more sensitive when compared to the normal counter current electrophoresis for the demonstration of mink antibodies against aleutian disease virus (adv). adv antigen can be produced from mink organs or in cell culture. the reactivity of the two types of antigen in the two kinds of counter current electrophoresis methods is described in this report. when 22 mink sera were titrated in normal counter current electrophore ... | 1982 | 7080835 |
| intrapulmonary lymphoid tissue in mink infected with aleutian disease virus. | pulmonary lymphoid tissue was studied histologically in mink infected experimentally and naturally with aleutian disease virus, an infection characterised by systemic lymphoid tissue hyperplasia. lymphoid tissue hyperplasia was observed in the lamina propria of the bronchial tree, in perivascular tissue, in interlobular septae and subpleurally by day 42 after experimental aerosol infection. four forms of pulmonary lymphoid tissue were recognised: lymphoepithelial nodules or bronchus-associated l ... | 1982 | 7079603 |
| immune complexes in aleutian disease: demonstration of antibody on isolated virus. | the specific binding of staphylococcal protein a for mammalian immunoglobulin g was used to demonstrate igg associated with aleutian disease virus (adv) when isolated from infected mink tissues. protein a specifically bound to mink serum ig with no reaction with other serum or tissue proteins. protein a labeled with 131iodine reacted with crude virus preparations but not with virus that had been purified by freon extraction to the point where it became reactive with antibody by counterimmunoelec ... | 1984 | 6328738 |
| mink with aleutian disease have high-affinity antiviral antibodies. | mink persistently infected with aleutian disease virus (adv) develop plasmacytosis (hypergammaglobulinaemia) and immune complex disease. mink of different colour phases were infected with different strains of adv and bled at different times after infection. the average antibody affinities (kav) were measured in the sera and found to fall in the range of 2 x 10(9) - 2 x 10(10) m-1, thus indicating good-quality antibodies. in sera of non-aleutian genotype mink a decline in kav during development o ... | 1984 | 6328642 |
| analysis of the quantity of antiviral antibodies from mink infected with different aleutian disease virus strains. | mink persistently infected with aleutian disease virus (adv) develop hypergammaglobulinaemia and immune complex disease. radiolabelled antibodies from mink infected with adv-g, dk, pullman , and utah i strains of adv were reacted against all four adv strains in radioimmunoassay (ria). the amount of anti-adv antibody in two equally hypergammaglobulinaemic serum pools varied from 13% (anti- pullman ) to 57% (anti-utah i). serum pools from two other sources (anti-dk and anti-adv-g), although less h ... | 1984 | 6328641 |
| immunoglobulin classes of aleutian disease virus antibody. | aleutian disease virus (adv) persistently infects mink and causes marked hypergammaglobulinemia. immunoglobulin class-specific antisera were used to define the total immunoglobulin of each class by radial immunodiffusion and the immunoglobulin class of adv-specific antibody by immunofluorescence in experimentally and naturally infected mink. electrophoretic gamma globulin closely reflects the immunoglobulin g (igg) level in mink, and the majority of the increased immunoglobulin and adv antibody ... | 1984 | 6319283 |
| molecular cloning of the aleutian disease virus genome: expression of aleutian disease virus antigens by a recombinant plasmid. | three nonoverlapping segments representing approximately 80% of the 4.8-kilobase pair aleutian disease virus (adv-g) duplex genome were molecularly cloned into either bacteriophage m13mp9 (m13bm2 = 0.07 to 0.15 map unit; m13bm1 = 0.15 to 0.54 map unit) or plasmid puc8 (pbm1 = 0.54 to 0.88 map units). in addition the 0.54- to 0.88-map unit segment of a danish isolate of adv (dk adv) was also cloned into puc8 (pbm2). the recombinant plasmids pbm1 and pbm2 induced expression of several polypeptides ... | 1983 | 6313959 |
| characterization of deoxyribonucleic acid from cells infected with aleutian disease virus. | viral dna was extracted from crandell feline kidney (crfk) cells infected with aleutian disease virus (adv) and labeled with [ 3h ]thymidine. the sedimentation coefficient in alkaline sucrose gradients was 16s corresponding to a molecular weight of 1.5 x 10(6). the buoyant densities of dna from infected and control cells were determined by isopyknic sedimentation in cscl and nai gradients. two additional peaks of [ 3h ]dna were found in infected cells, but not in control cell extracts. fractiona ... | 1983 | 6309040 |
| an easy method for comparing antibody affinities to related antigens. | four different aleutian disease virus (adv) isolates were coated to 1/4" polystyrene balls. the binding capacity of these balls against different mouse hybridoma antibodies prepared against each of the adv isolates was investigated in two ways. the different adv-coated balls were either mixed in a tube (competition between balls for antibody), or they were allowed to react separately with antibody (no competition). the difference in competitive and non-competitive binding values allows a compari ... | 1983 | 6306992 |
| characterization of the aleutian disease virus genome and its intracellular forms. | aleutian disease virus (adv) of mink is a nondefective parvovirus with a single-stranded dna genome. we characterized the viral dna forms found in infected cells prepared by a modified hirt extraction procedure. double-stranded dna molecules corresponding in size to 4.8-kilobase-pair duplex monomers and 9.6-kilobase-pair duplex dimers were identified in agarose gels by blot hybridization to 32p-labeled adv dna. a rapidly reannealing adv duplex monomer was isolated on a preparative scale and phys ... | 1983 | 6300467 |
| isolation of virus and antibody containing immune complexes from mink with aleutian disease by affinity chromatography of equine complement clq. | affinity chromatography on immobilized equine complement clq was used for the isolation of complement-binding immune complexes in sera of mink infected with aleutian disease virus. immune complexes were isolated and quantitated from 4 of 5 infected mink, as early as 2 weeks after infection and before hypergammaglobulinemia had appeared. the quantity of immunoglobulin g in these immune complexes ranged from 180 to 370 micrograms/ml serum. there were no clq-binding immune complexes found in mink w ... | 1983 | 6297342 |
| identification of a nonvirion protein of aleutian disease virus: mink with aleutian disease have antibody to both virion and nonvirion proteins. | we studied aleutian disease virus polypeptides in crandall feline kidney (crfk) cells. when crfk cells labeled with [35s]methionine at 60 h postinfection were studied by immunoprecipitation with sera from infected mink, the major aleutian disease virus virion polypeptides (p85 and p75) were consistently identified, as was a 71,000-dalton nonvirion protein (p71). the peptide maps of p85 and p75 were similar, but the map of p71 was different. p85, p75, and p71 were all precipitated by sera from al ... | 1982 | 6287034 |
| detection of antibody in aleutian disease of mink: comparison of enzyme-linked immunosorbent assay and counterimmunoelectrophoresis. | experiments were undertaken to investigate the potential of the enzyme-linked immunosorbent assay (elisa) as a screening test for the diagnosis of the 2 known naturally occurring forms of aleutian disease of mink. anti-aleutian disease virus (adv) antibody activity was not detectable in the sera of mink with nonprogressive aleutian disease despite the demonstration of antibody by counterimmunoelectrophoresis (ciep) in the same sera. anti-adv antibody was detectable in 93% of sera from mink at v ... | 1982 | 6283966 |
| evaluation of chemical disinfectants for aleutian disease virus of mink. | nine chemicals and commercial disinfectants were tested for inactivation of aleutian disease virus of mink. in the presence of distilled water, a commercial disinfectant (o-syl), halogen derivatives (iodophor and sodium hypochlorite), and glutaraldehyde (2.0%) inactivated 4 log10 (based on 0.25 ml) of the virus within 10 minutes at 23 c. formalin (2.0%) and o-syl were slower to inactivate the virus, but achieved a 4 log10 reduction in titer by 30 minutes' contact time. in the presence of 10% bov ... | 1981 | 6266294 |
| purification and characterization of aleutian disease virus. | virus was isolated from infected mink organs by a combination of tissue homogenization, fluorocarbon extraction and ultracentrifugation. the final preparation was analysed by crossed immunoelectrophoresis and electronmicroscopy. virions had a capsid diameter of 22 nm. preparative agarose electrophoresis separated virions from contaminating ferritin. crossed immunoelectrophoresis of virus gave a single precipitate with sera from infected mink. crossed immunoelectrophoretic analysis with intermedi ... | 1980 | 6261524 |
| anti-deoxyribonucleic acid antibody associated with persistent infection of mink with aleutian disease virus. | anti-deoxyribonucleic acid (dna) antibody was quantitated in sera from mink infected with aleutian disease virus (adv). during the course of the disease after experimental infection, the amount of anti-dna antibody increased 60% initially, but then decreased to an intermediate level when measured 2.5 months later. the percentage of serum immunoglobulin, however, steadily increased over 3.5-fold during this period, resulting in the characteristic gammopathy. correlation between the level of anti- ... | 1980 | 6260652 |
| antibodies to aleutian disease virus in human sera. | 1980 | 6255045 | |
| characterization of aleutian disease virus as a parvovirus. | we characterized a strain of aleutian disease virus adapted to growth in crandall feline kidney cells at 31.8 degrees c. when purified from infected cells, aleutian disease virus had a density in cscl of 1.42 to 1.44 g/ml and was 24 to 26 nm in diameter. [3h]thymidine could be incorporated into the viral genome, and the viral dna was then studied. in alkaline sucrose gradients, aleutian disease virus dna was a single species that cosedimented at 15.5s with single-stranded dna from adeno-associat ... | 1980 | 6252342 |
| aleutian disease virus, a parvovirus, is proteolytically degraded during in vivo infection in mink. | the polypeptides of the highly virulent mink-passaged utah i and the nonvirulent cell culture-adapted adv-g strain of aleutian disease virus (adv) were compared. when crfk cells infected with either utah i or adv-g were analyzed by immunoprecipitation, both viruses induced proteins with molecular weights characteristic of the adv-g 85,000 ( 85k )- and 75k-dalton structural proteins (p85 and p75) as well as the 71k -dalton nonvirion protein p71 . however, when utah i, pullman adv, and dk adv (a d ... | 1984 | 6202887 |
| serological analyses of different mink aleutian disease virus strains. | four different isolates of aleutian disease virus have been compared electrophoretically and serologically. these were the dk and utah 1 isolates, known as highly virulent strains, the pullman isolate, known as a low virulent strain and the avirulent adv-g isolate, which is the only strain grown in cell culture. adv-g was shown to migrate in agarose electrophoresis 22 per cent slower than the other strains. several murine monoclonal antibodies were prepared against each of the isolates. each one ... | 1984 | 6200092 |
| royal pastel mink respond variously to inoculation with aleutian disease virus of low virulence. | information was sought on the varied responses of royal pastel mink (a non-aleutian genotype) to aleutian disease virus of low virulence. thus, of 20 yearling female pastel mink inoculated subcutaneously with a large amount of the pullman strain of aleutian disease virus, only 3 succumbed to the disease. of the other 17 mink, 3 had neither viremia nor a rise in level of serum gamma globulin during the 24 weeks after inoculation. the other 14 mink were viremic for variable periods during the firs ... | 1984 | 6199516 |
| sensitive radioimmune assay for measuring aleutian disease virus antigen and antibody. | a solid-phase, one-step radioimmune assay was developed which could detect as little as 0.02 microliter of a standard aleutian disease virus antigen preparation, approximately 3.2 ng of viral protein. virus antigen was measured in different mink organs and cell types during experimental intraperitoneal infection. the gut and kidney were the first organs in which virus antigen could be detected (day 3 to 6 after infection). on day 6 or later virus antigen was found in spleen, liver, kidney, lymph ... | 1983 | 6195178 |
| demonstration of aleutian disease virus-specific lymphocyte response in mink with progressive aleutian disease: comparison of sapphire and pastel mink infected with different virus strains. | lymphocyte blastogenesis was used to study the antiviral lymphocyte response of sapphire (aleutian) and pastel (nonaleutian) mink inoculated with pullman or utah 1 aleutian disease virus (adv). both mink genotypes developed a virus-specific response when inoculated with utah 1 adv. in contrast, after inoculation of pullman adv, sapphire mink had a positive virus-specific response, whereas pastel mink did not. response occurred late after infection (8 wk) and correlated with the development of pr ... | 1983 | 6193194 |
| comparative pathogenicity of four strains of aleutian disease virus for pastel and sapphire mink. | information was sought on the comparative pathogenicity of four north american strains (isolates) of aleutian disease virus for royal pastel (a non-aleutian genotype) and sapphire (an aleutian genotype) mink. the four strains (utah-1, ontario [canada], montana, and pullman [washington]), all of mink origin, were inoculated intraperitoneally and intranasally in serial 10-fold dilutions. as indicated by the appearance of specific antibody (counterimmunoelectrophoresis test), all strains readily in ... | 1983 | 6193063 |
| autoimmunity in aleutian disease: contribution of antiviral and anti-dna antibody to hypergammaglobulinemia. | the contributions to aleutian disease gammopathy of specific antiviral antibody and an autoimmune component, anti-dna antibody, were studied with pastel ranch mink naturally infected with aleutian disease virus. specific antibody activities were determined by countercurrent immunoelectrophoresis and radioimmune assay, respectively. gamma globulin levels (percent gamma) were determined by serum electrophoresis. within an infected mink population, it was possible to predict the level of gammopathy ... | 1983 | 6192083 |
| aleutian disease in ferrets. | when 32 antibody-free ferrets were inoculated with the highly mink-virulent utah-1 strain of aleutian disease virus (adv), most developed adv antibody starting 15 days after infection, but the antibody titers were much lower than those seen in mink. relatively small amounts of adv were demonstrated in crfk cell culture, using ferret spleen and lymph node homogenates only 4 to 10 days after experimental infection, but low-level viral persistence for 180 days was shown by mink inoculation. the fer ... | 1982 | 6176546 |
| much of the increased igg in aleutian disease of mink is viral antibody. | aleutian disease (ad) is caused by a persistent infection of mink with an autonomous parvovirus. chronically infected mink develop widespread plasmacytosis, a marked elevation of their serum igg, and immune complex disease. a substantial fraction of the igg in the serum of mink with aleutian disease may be specifically absorbed by monolayer cell cultures infected with aleutian disease virus. the maximum percentage of absorption of igg found was 81% in a mink with 5.4 g/dl of igg. mink with the m ... | 1984 | 6100982 |
| examination of aleutian disease virus in charge-shift crossed immunoelectrophoresis. | the surface properties of aleutian disease virus were studied by charge-shift crossed immunoelectrophoresis. when different strains of aleutian disease virus were treated with non-charged detergent followed by charged detergents, they showed bi-directional migration velocity shifts in electrophoresis, indicating amphiphilic surface properties of the virus. | 1984 | 6099679 |
| aleutian disease in mink: virology, immunology and pathogenesis. | aleutian disease is an immunological disease of mink caused by a persistent virus infection. recently aleutian disease viral antigen has been extracted from tissue of mink early in the course of infection and the aleutian disease virus has been isolated. the virus particles were similar in morphology and size to certain parvovirus having a 23 nm diameter, spherical shape and icosahedral capsid. recent developments in serological techniques, i.e. immunofluorescence, complement fixation, countercu ... | 1984 | 6096541 |
| aleutian disease virus in b and t lymphocytes from blood and spleen and in bone marrow cells from naturally infected mink. | in the nuclei of 4% of peripheral blood or spleen mononuclear cells (mnc), aleutian disease virus(adv)-specific antigens were found by a direct immunofluorescence test. the mnc were further fractionated by nylon wool, affinity chromatography using staphylococcus aureus protein, or percoll gradient techniques. adv and specific antigens were detected in mnc fractions enriched in either the b or t lymphocytes. in the bone marrow, up to 40% antigen-positive cells were demonstrated over a period of 1 ... | 1984 | 6096293 |
| acute interstitial pneumonitis caused by aleutian disease virus in mink kits. | in four danish mink ranches acute interstitial pneumonitis caused excessive mortality among kits within the first 2 1/2 months after parturition. the disease was found to be due to an aleutian disease virus (adv) and could be reproduced experimentally in neonatal kits by inoculation with material from spontaneous cases, as well as with other strains of adv. experimental reproduction was only possible in kits from dams free of aleutian disease (ad) whereas kits from dams experimentally or natural ... | 1984 | 6095587 |
| immunoenzyme western blotting analysis of antibody specificity in aleutian disease of mink, a parvovirus infection. | aleutian disease virus (adv), an autonomous parvovirus, persistently infects mink and induces very high levels of virus-specific antibody. all strains of adv infect all mink, but only highly virulent strains cause progressive disease in non-aleutian mink. the development of antibody to individual adv proteins was evaluated by western blotting by using the sera of 22 uninfected mink and 163 naturally or experimentally infected mink. adv has virion proteins of 86,000 and 78,000 daltons that are cl ... | 1984 | 6092706 |
| the pathogenesis of aleutian disease of mink. i. in vivo viral replication and the host antibody response to viral antigen. | mink inoculated with 1 x 10(5)id(50) of aleutian disease virus revealed very high virus titers in the tissues 8-18 days later. the highest virus titers observed were 5 x 10(8)id(50) per g of spleen and 1 x 10(9)id(50) per g of liver 10 days after inoculation. concomitant with the increase in infectious virus titers, viral antigen(s) was found in the cytoplasm of macrophages in the spleen and lymph nodes and in kupffer cells in the liver. antiviral antibody was assayed by indirect immunofluoresce ... | 1969 | 4979953 |
| the persistence of aleutian disease virus in the mosquito aedes fitchii. | 1973 | 4633583 | |
| isolation of aleutian mink disease virus by affinity chromatography. | affinity chromatography was used to isolate the aleutian disease virus of mink. dissociation of the immunoadsorbent-virus complex with 0.75 molar sodium chloride and then with a glycine-hydrochloride gradient released infective particles resembling picornaviruses. the elution profile suggests that two different types of virus-antibody complexes are formed, one dissociated by sodium chloride and another that requires glycine-hydrochloride in addition to sodium chloride for release of virus. | 1973 | 4629857 |
| antibody-forming cells and serum hemolysin responses of pastel and sapphire mink inoculated with aleutian disease virus. | the effect of aleutian disease virus (adv) on serum hemolysin titers and antibody-forming cells in lymph nodes and spleens of sapphire and pastel mink inoculated with goat erythrocytes (g-rbc) was investigated. adv injected 1 day after primary antigenic stimulation with g-rbc did not depress the immune responses of either color phase for a period of 26 days. however, when g-rbc were injected 47 days after adv, both the number of antibody-forming cells and hemolysin titers were more markedly depr ... | 1973 | 4584051 |
| the pathogenesis of aleutian disease of mink. 3. immune complex arteritis. | mink chronically infected with aleutian disease virus develop a severe necrotizing arteritis affecting muscular arteries. acute, subacute and healing lesions may be found. extracellular deposits of host immunoglobulin and complement and, after acid elution, viral antigen can be shown by immunofluorescence technics in areas of fibrinoid necrosis and between proliferating endothelial cells. no intracellular viral antigen was found, indicating that the virus probably does not replicate in vascular ... | 1973 | 4576760 |
| aleutian disease of mink. prevention of lesions by immunosuppression. | mink that were homozygous recessive for the aleutian gene (aa) were inoculated with aleutian disease virus (adv) and simultaneously treated with cyclophosphamide (cy). control mink were inoculated with adv. all mink were injected with bovine serum albumin (bsa) and their anti-bsa antibody response was measured to monitor the influence of drug therapy on the humoral antibody response. formation of anti-bsa antibody was markedly suppressed and the hypergammaglobulinemia and development of ad lesio ... | 1972 | 4551296 |
| demonstration of aleutian mink disease virus in cell culture. | 1973 | 4201043 | |
| pathogenesis of aleutian disease of mink: nature of the antiglobulin reaction and elution of antibody from erythrocytes and glomeruli of infected mink. | erythrocytes from mink chronically infected with aleutian disease virus (adv) gave positive antiglobulin reactions with rabbit anti-mink immunoglobulin (ig)g, anti-mink c3, and anti-mink serum, but did not react with anti-mink igm. the strongest reaction was observed with anti-mink c3. immunoelectrophoresis demonstrated that serum from rabbits injected with erythrocytes from adv-infected mink gave a precipitin line with normal mink serum in the beta globulin region corresponding to c3. when norm ... | 1973 | 4199157 |
| isolation, purification and structure of aleutian disease virus by immunological techniques. | 1973 | 4197346 | |
| the immunoglobulins in aleutian disease (viral plasmacytosis) of mink. different types of hypergammaglobulinemias. | mink infected with the aleutian disease virus developed different types of hypergammaglobulinemias according to the incidence of immunoglobulin classes. usually, increases of immunoglobulin in serum of infected mink occurred over a broad spectrum as a general increase in the immunoglobulins. in some infected animals a predominantly selective increase was demonstrated with only certain immunoglobulin classes being present at elevated levels. selective increases of immunoglobulin classes were obse ... | 1970 | 4097854 |
| natural antibodies in sera of mink before and after the development of aleutian disease (viral plasmacytosis). | the effect of infection of mink with aleutian disease virus on the level of natural antibodies in the serum was investigated. the level of natural antibodies to chicken red blood cells was increased following infection but there was no correlation between the degree of hypergamma globulinemia in the diseased mink and the increase in titers. on the other hand, serum levels of natural hemolytic antibodies to sheep red blood cells in mink did not increase during the course of aleutian disease. thes ... | 1970 | 4097853 |
| analysis of aleutian disease virus infection in vitro and in vivo: demonstration of aleutian disease virus dna in tissues of infected mink. | aleutian disease virus (adv) infection was analyzed in vivo and in vitro to compare virus replication in cell culture and in mink. initial experiments compared cultures of crandell feline kidney (crfk) cells infected with the avirulent adv-g strain or the highly virulent utah i adv. the number of adv-infected cells was estimated by calculating the percentage of cells displaying adv antigen by immunofluorescence (ifa), and several parameters of infection were determined. infected cells contained ... | 1985 | 3894686 |
| pregnancy rate and foetal mortality in aleutian disease virus infected mink. | 1988 | 3223478 | |
| in situ molecular hybridization for detection of aleutian mink disease parvovirus dna by using strand-specific probes: identification of target cells for viral replication in cell cultures and in mink kits with virus-induced interstitial pneumonia. | strand-specific hybridization probes were utilized in in situ molecular hybridization specifically to localize replicative form dna of aleutian mink disease parvovirus (adv). throughout in vitro infection, duplex replicative form dna of adv was located in the cell nuclei. single-stranded virion dna and capsid proteins were present in the nuclei early in infection, but were later translocated to the cytoplasm. in neonatal mink, adv causes acute interstitial pneumonia, and replicative forms of vir ... | 1987 | 3037104 |
| lymphotropic strain sl3 of aleutian disease virus: identification of replicative form dna, molecular cloning and expression of capsid-specific proteins. | replicative form (rf) dna of the lymphotropic strain sl3 of aleutian disease virus was isolated from infected cell cultures. a novel intermediate of about 7.6 kilobases was demonstrated in hirt lysates in addition to single-stranded viral, double-stranded monomer and dimer rf dna. the monomer rf dna exhibited a length heterogeneity of 70 bp and 160 bp at its 3' and 5' termini. the two major monomer rf dna species each contained hairpins in the extended or the foldback configurations. a central f ... | 1987 | 3033124 |
| replication of aleutian disease virus in mink lymphocytes infected in vitro. | the kinetics of aleutian disease virus (adv) replication in mink lymphocytes was followed by the analysis of virus-specific antigens, infectious virus, and viral dna. stimulation with pokeweed mitogen (pwm; 20 micrograms/ml) increased the synthesis of adv dna in cultivated cells of the b-cell fraction. maximum virus titers [10(6.5) fluorescence-forming units (ffu)/ml] were achieved after incubating infected cells for 60 h at 32 degrees. at this time, 10.4% of the cells in the b-cell-enriched fra ... | 1986 | 3026990 |
| restricted heterogeneity of the early antibody response to aleutian disease virus in mink kits. | mink kits born of aleutian disease (ad) negative dams were infected neonatally with different isolates of aleutian disease virus (adv). they were then sacrificed at different days after infection (10-45 days) and their sera were analysed by different techniques for concentrations of immunoglobulins and quality of the produced antibodies to adv. the infected mink kits produced significantly higher concentrations of igm and igg immunoglobulins than non-infected-age matched control mink (p less tha ... | 1986 | 3024449 |
| studies on the sequential development of acute interstitial pneumonia caused by aleutian disease virus in mink kits. | we studied different parameters during the development of acute interstitial pneumonia in mink kits caused by neonatal infection with aleutian disease virus (adv). when histological lesions, presence of intranuclear inclusion bodies, and intranuclearly localized adv antigen were correlated with levels of single-stranded virion and duplex replicative forms of adv dna in the different tissues, it was concluded that the lung, probably alveolar type ii cells, is the major primary target for viral re ... | 1987 | 3023709 |
| acute interstitial pneumonia in mink kits: experimental reproduction of the disease. | organ homogenates from kits that died of interstitial pneumonia were inoculated into adult aleutian disease virus (adv)-negative mink and shown to contain infectious adv. acute interstitial pneumonia was experimentally reproduced with the organ homogenate but only by inoculation of newborn kits born from adv-negative dams. older kits and kits from adv-positive dams did not develop interstitial pneumonia, but later developed the classic form of aleutian disease. electron microscopic examination w ... | 1986 | 3022453 |
| demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report. | isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ... | 1986 | 3002299 |
| monoclonal antibodies against aleutian disease virus distinguish virus strains and differentiate sites of virus replication from sites of viral antigen sequestration. | monoclonal antibodies (mabs) were used to study antigenic differences among strains of aleutian disease virus (adv) and to characterize viral proteins in vitro and in vivo. a number of adv field strains could be discriminated, and highly virulent utah i adv was clearly delineated from the tissue culture-adapted avirulent adv-g strain. this specificity could be demonstrated by indirect immunofluorescence against infected cultures of crandell feline kidney cells or against tissues of utah i adv-in ... | 1986 | 3001352 |
| experimental transmission of aleutian disease virus (adv) to different animal species. | two animals from each of 8 different species (mink, finn raccoon, cat, dog, ferret, blue fox, mouse and rabbit) were inoculated with the highly virulent utah i strain of adv. only the mink developed hypergammaglobulinemia. all animals produced antibodies to adv antigens, but with different titres. mink sera had much higher antibody titres than the other animal sera. antibodies to the adv-coded, non-structural polypeptide (p71) were found in mink, finn raccoons and dogs only. presence of this kin ... | 1985 | 2994362 |
| temporal replication of the pullman strain of aleutian disease virus in royal pastel mink. | information was sought on the temporal replication of aleutian disease virus in 27 royal pastel mink. groups of three were examined 8 to 126 days after they were inoculated subcutaneously with 10(3) 50% lethal doses of the pullman strain. much individual variation was noted in the onset of infection, occurrence of viremia, and extent of virus replication in the tissues. thus, virus was detected in lymph nodes regional to the site of inoculation in only some mink during the first 14 days after in ... | 1985 | 2991603 |
| rocket line immunoelectrophoresis: an improved assay for simultaneous quantification of a mink parvovirus (aleutian disease virus) antigen and antibody. | a rocket line immunoelectrophoretic assay (rlie) was developed for the simultaneous quantification of viral antigens and antiviral antibodies of the important mink parvovirus, aleutian disease virus (adv). the sensitivity of the rlie assay was found to be 5 log2 higher than that of the counter current immunoelectrophoresis which is the assay routinely used for diagnostic purposes. | 1985 | 2984229 |
| detailed transcription map of aleutian mink disease parvovirus. | we studied the transcription program of aleutian mink disease parvovirus (adv) by using a combination of cdna cloning and sequencing, primer extension, and northern (rna) blot hybridization with splice-specific oligonucleotides. the 4.8-kilobase adv genome was transcribed in the rightward direction, yielding plus-sense polyadenylated transcripts of 4.3 (r1 rna), 2.8 (r2), 2.8 (r3), 1.1 (rx), and 0.85 (r2') kilobases. each rna transcript had potential translation initiation sites within open read ... | 1988 | 2843669 |
| capsid protein vp1 (p85) of aleutian disease virus is a major dna-binding protein. | the interaction between aleutian disease virus (adv) dna and proteins isolated from adv-infected cells or adv virions, respectively, was examined. proteins were separated on sds-polyacrylamide gels, transferred to nitrocellulose, and probed with 32p-labeled restriction fragments of replicative form (rf) dna or with single-stranded virion dna. the adv capsid protein vp1 was found to bind the 3'-terminal bamhi fragment of rf dna extending from map units (m.u.) 0 to 15. no binding was observed with ... | 1988 | 2842956 |
| nucleotide sequence and genomic organization of aleutian mink disease parvovirus (adv): sequence comparisons between a nonpathogenic and a pathogenic strain of adv. | a dna sequence of 4,592 nucleotides (nt) was derived for the nonpathogenic adv-g strain of aleutian mink disease parvovirus (adv). the 3'(left) end of the virion strand contained a 117-nt palindrome that could assume a y-shaped configuration similar to, but less stable than, that of other parvoviruses. the sequence obtained for the 5' end was incomplete and did not contain the 5' (right) hairpin structure but ended just after a 25-nt a + t-rich direct repeat. features of adv genomic organization ... | 1988 | 2839709 |
| detection of aleutian disease virus dna in tissues of naturally infected mink. | organs of naturally infected mink were examined for the presence of aleutian disease virus (adv) dna by in situ hybridization. spleen, lymph nodes, thymus, bone marrow, kidney, liver, lung and small intestine were found to be positive for adv to differing extents. infected lymphoid organs showed a focal distribution of positive cells. southern blot analysis of dna extracted from infected organs revealed replicative forms of viral dna in spleen and bone marrow samples only. these findings are con ... | 1988 | 2832533 |
| mink infected with aleutian disease virus have an elevated level of cd8-positive t-lymphocytes. | lymphocytes, monocytes, granulocytes, b-lymphocytes and cd8-positive t-lymphocytes of non-infected mink and mink infected with aleutian disease virus (adv) were measured by flow cytometry. the gammaglobulin levels of the sera were also measured. besides development of hypergammaglobulinaemia in the infected mink, the most pronounced finding was that the number of cd8-positive lymphocytes doubled on average during development of aleutian disease, while the number of b-lymphocytes did not change d ... | 1989 | 2785727 |