Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| non-injected illicit drug use and infectious disease risk of donor tissue: a single institution retrospective review. | this study assessed the relationship of non-injected illicit drug use and infectious disease seropositivity for human immunodeficiency virus (hiv-1), hepatitis b virus (hbv), hepatitis c virus (hcv), and syphilis. in a retrospective review of 986 donor charts recovered from 2009 to 2011 at a single tissue bank, the absence of reported non-injected illicit drug use corresponded with seropositivity in 6.61 %, of recovered donors while reported illicit drug use in the medical and social history cor ... | 2015 | 26006785 |
| snapshot and ce-sscp: two simple and cost-effective methods to reveal genetic variability within a virus species. | the multiplex snapshot and the capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) procedures are here used for rapid and high-throughput description of the molecular variability of viral populations. both approaches are based on (1) standard amplification of genomic sequence(s), (2) labeled primers or labeled single-stranded dna, and (3) migration of fluorescent-labeled molecules in capillary electrophoresis system. the snapshot technology was used to describe the divers ... | 2015 | 25981256 |
| respiratory viruses in airline travellers with influenza symptoms: results of an airport screening study. | there is very little known about the prevalence and distribution of respiratory viruses, other than influenza, in international air travellers and whether symptom screening would aid in the prediction of which travellers are more likely to be infected with specific respiratory viruses. | 2015 | 25959149 |
| can epstein-barr virus dna load in nasopharyngeal brushings or whole blood predict recurrent nasopharyngeal carcinoma in a non-endemic region? a prospective nationwide study of the dutch head and neck oncology cooperative group. | this study estimated the value of quantitative measurements of ebv markers in the clinical management of nasopharyngeal carcinoma in a non-endemic area. the aim was to predict prognosis and detect recurrent and residual disease. in 72 patients, ebv dna load in blood and nasopharyngeal brushes, and iga vca-p18 and ebna1 in plasma were measured at different time points. at diagnosis and post-treatment, a cut-off value was used for detecting disease [positive (ppv) and negative (npv) predictive val ... | 2016 | 25929413 |
| serological prevalence of viral agents that induce reproductive failure in south korean wild boar. | viral agents associated with reproductive failure such as aujeszky's disease virus (adv), encephalomyocarditis virus (emcv), and porcine parvovirus (ppv) have also been identified in european wild boar. to screen for the presence of antibodies against adv, emcv, and ppv from wild boar (sus scrofa) in south korea, 481 serum samples were collected from wild boar hunted between december 2010 and may 2011. | 2015 | 25888836 |
| clinical characteristics and biopsy accuracy in suspected cases of sjögren's syndrome referred to labial salivary gland biopsy. | labial salivary gland biopsy (lsgb) is the most important diagnostic tool for the diagnosis of sjögren's syndrome (ss), but its diagnostic value is rarely studied. this study assessed the sensibility and specificity of lsgb, and the clinical profiles of patients who were referred for biopsy. | 2015 | 25887888 |
| use of real time polymerase chain reaction for detection of m. tuberculosis, m. avium and m. kansasii from clinical specimens. | the incidence of m. tuberculosis (mtb) and non tuberculous mycobacterium species (ntms) like m. avium and m. kansasii has increased due to human immunodeficiency virus (hiv) epidemic. therefore accurate, rapid and cost effective methods for the identification of these ntms and mtb are greatly needed for appropriate tb management. thus in this study we evaluated the performance of lightcycler(®) mycobacterium detection assay to detect mtb, m. avium and m. kansasii in sputum specimens. | 2015 | 25884439 |
| rapid and specific detection of porcine parvovirus using real-time pcr and high resolution melting (hrm) analysis. | porcine parvovirus (ppv) is the important causative agent for infectious infertility, which is a fairly tough virus that multiplies normally in the intestine of pigs without causing clinical signs in the world. | 2015 | 25879634 |
| enveloped virus flocculation and removal in osmolyte solutions. | our ability to reduce infectious disease burden throughout the world has been greatly improved by the creation of vaccines. however, worldwide immunization rates are low. the two most likely reasons are the lack of sufficient distribution in underdeveloped countries and the high cost of vaccine products. the high costs are due to the difficulties of manufacturing individual vaccine products with specialized purification trains. in this study, we propose to use virus flocculation in osmolytes, fo ... | 2015 | 25865274 |
| the role of polymerase chain reaction of high-risk human papilloma virus in the screening of high-grade squamous intraepithelial lesions in the anal mucosa of human immunodeficiency virus-positive males having sex with males. | to evaluate the advantages of cytology and pcr of high-risk human papilloma virus (pcr hr-hpv) infection in biopsy-derived diagnosis of high-grade squamous intraepithelial lesions (hsil = ain2/ain3) in hiv-positive men having sex with men (msm). | 2015 | 25849412 |
| in vitro antiviral activity of germacrone against porcine parvovirus. | porcine parvovirus (ppv) infections can lead to significant losses to the swine industry by causing reproductive failure in pigs. germacrone has been reported to efficiently suppress the replication of influenza virus. in this report, the antiviral activity of germacrone on ppv in swine testis (st) cells was investigated. here, we show for the first time that germacrone protects cells from ppv infection and suppresses the synthesis of viral mrna and protein. furthermore, we show that germacrone ... | 2015 | 25813663 |
| clinical utility of urinary cytology to detect bk viral nephropathy. | reactivation of bk polyoma virus can result in destructive viral allograft nephropathy (bkvan) with limited treatment options. screening programs using surrogate markers of viral replication are important preventive strategies, guiding immunosuppression reduction. | 2015 | 25769077 |
| hbsag quantification in clinical practice. | several standardized commercial assays for quantification of hepatitis b surface antigen (qhbsag) are now available. studies on hbsag kinetics from asia and europe have demonstrated that hbsag levels are highest during the immune-tolerant phase, become lower during immune-clearance phase and are the lowest in hepatitis b 'e' antigen (hbeag)-negative inactive low-replicative phase with a rise during hbeag-negative chronic hepatitis b (chb). combined use of hepatitis b virus-deoxyribonucleic acid ... | 2012 | 25755408 |
| predicting mortality across a broad spectrum of liver disease-an assessment of model for end-stage liver disease (meld), child-turcotte-pugh (ctp), and creatinine-modified ctp scores. | the role of model for end-stage liver disease (meld) among indian patients with cirrhosis is uncertain. we studied and compared meld with child-turcotte-pugh (ctp) and creatinine-modified-ctp (crctp) scores for predicting 1-, 3-, and 6-months mortality. | 2011 | 25755381 |
| utility of hepatitis c viral load monitoring on direct-acting antiviral therapy. | hepatitis c virus (hcv) rna loads serve as predictors of treatment response during interferon-based therapy. we evaluated the predictive ability of hcv rna levels at end of treatment (eot) for sustained virologic response (svr12) during interferon-sparing direct-acting antiviral therapies. | 2015 | 25733369 |
| parapoxvirus (ppv) of red deer reveals subclinical infection and confirms a unique species. | parapoxvirus (ppv) infections are of worldwide importance, particularly in sheep and goat herds. owing to the zoonotic potential of all ppv species, they are a permanent threat to human health as well. the virus is also known to affect wildlife, as reported for pinnipeds, red deer and several other wild ruminants. ppvs found in red deer have been claimed as a unique species according to certain genomic features. so far infection of wildlife has been recognized because of clinical manifestation s ... | 2015 | 25701822 |
| comparative sequence analysis of double stranded rna binding protein encoding gene of parapoxviruses from indian camels. | the dsrna binding protein (rbp) encoding gene of parapoxviruses (ppvs) from the dromedary camels, inhabitating different geographical region of rajasthan, india were amplified by polymerase chain reaction using the primers of pseudocowpoxvirus (pcpv) from finnish reindeer and cloned into pgem-t for sequence analysis. analysis of rbp encoding gene revealed that ppv dna from bikaner shared 98.3% and 76.6% sequence identity at the amino acid level, with pali and udaipur ppv dna, respectively. refer ... | 2014 | 25685494 |
| mechanistic evaluation of virus clearance by depth filtration. | virus clearance by depth filtration has not been well-understood mechanistically due to lack of quantitative data on filter charge characteristics and absence of systematic studies. it is generally believed that both electrostatic interactions and sized based mechanical entrapment contribute to virus clearance by depth filtration. in order to establish whether the effectiveness of virus clearance correlates with the charge characteristics of a given depth filter, a counter-ion displacement techn ... | 2015 | 25683459 |
| antiviral effect of lithium chloride on infection of cells by porcine parvovirus. | porcine parvovirus (ppv) causes reproductive failure in pigs, which leads to economic losses to the industry. as reported previously, licl efficiently impairs the replication of a variety of viruses, including the coronavirus infectious bronchitis virus (ibv), transmissible gastroenteritis virus (tgev), and pseudorabies herpesvirus. we demonstrate for the first time that inhibition of ppv replication in swine testis (st) cells by licl is dose-dependent, and that the antiviral effect of licl occu ... | 2015 | 25663217 |
| the potyviridae p1a leader protease contributes to host range specificity. | the p1a protein of the ipomovirus cucumber vein yellowing virus is one of the self-cleavage serine proteases present in potyviridae family members. p1a is located at the n-terminal end of the viral polyprotein, and is closely related to potyviral p1 protease. for its proteolytic activity, p1a requires a still unknown host factor; this might be linked to involvement in host specificity. here we built a series of constructs and chimeric viruses to help elucidate the role of p1a cleavage in host ra ... | 2015 | 25562450 |
| serum levels of hepatitis b surface antigen predict severity of fibrosis in patients with e antigen-positive chronic hepatitis b. | noninvasive techniques are needed to assess hepatic fibrosis in patients with chronic hepatitis b. we developed a scoring system to determine the degree of fibrosis in patients with genotype b or genotype c hepatitis b virus (hbv) infection and positive for the hepatitis b e antigen. | 2015 | 25542306 |
| predictors of viral pneumonia in patients with community-acquired pneumonia. | viruses are increasingly recognized as major causes of community-acquired pneumonia (cap). few studies have investigated the clinical predictors of viral pneumonia, and the results have been inconsistent. in this study, the clinical predictors of viral pneumonia were investigated in terms of their utility as indicators for viral pneumonia in patients with cap. | 2014 | 25531901 |
| effect of plum pox virus on chemical composition and fruit quality of plum. | the quality of fruit from ppv (plum pox virus)-infected plum (prunus domestica l.) trees was investigated during the last 3 weeks of ripening using healthy tree (t3), tree with short-term infection (t2), and tree with long-term infection (t1). the pomological variables (presence of necrosis on fruit, color of fruit, color of flesh, firmness, soluble solids content, and fruit weight) and composition of nutritive compounds (sugars and organic acids) and bioactive compounds (phenolics: anthocyanins ... | 2015 | 25495040 |
| development and validation of new diagnostic criteria for acute retinal necrosis. | the purposes of this study are to develop and validate new diagnostic criteria for acute retinal necrosis (arn) based on the ocular findings, clinical course, and virologic testing of intraocular fluids. | 2015 | 25492579 |
| the p25 protein of potato virus x (pvx) is the main pathogenicity determinant responsible for systemic necrosis in pvx-associated synergisms. | most plant viruses counter the rna silencing-based antiviral defense by expressing viral suppressors of rna silencing (vsrs). in this sense, vsrs may be regarded as virulence effectors that can be recognized by the host as avirulence (avr) factors to induce r-mediated resistance. we made use of agrobacterium-mediated transient coexpression of vsrs in combination with potato virus x (pvx) to recapitulate in local tissues the systemic necrosis (sn) caused by pvx-potyvirus synergistic infections in ... | 2015 | 25473046 |
| serum and bal beta-d-glucan for the diagnosis of pneumocystis pneumonia in hiv positive patients. | the diagnosis of patients with pulmonary infiltrates and human immunodeficiency virus (hiv) infection remains a challenge. in current clinical practice the gold standard for pneumocystis jirovecii pneumonia (pcp) diagnosis remains the identification of the organism in bronco alveolar lavage (bal) using microscopy (e.g., silver stain). (1->3)-β -d-glucan (bg) is a polysaccharide that is present within the cell wall of pneumocystis and other fungi. | 2014 | 25448310 |
| association between oral candidiasis and low cd4+ count among hiv positive patients in hoima regional referral hospital. | the aim of this study was to determine the prevalence of human immune virus (hiv) related oral lesions and their association with cluster of differentiation 4 (cd4+) count among treatment naïve hiv positive patients. | 2014 | 25432363 |
| [diagnostic value of urine hbv dna for hepatitis b virus-associated glomerulonephritis]. | to investigate the potential value of urine hepatitis b virus (hbv) dna as a new noninvasive diagnostic indicator for hbv-associated glomerulonephritis (hbv-gn). | 2014 | 25413080 |
| limits in virus filtration capability? impact of virus quality and spike level on virus removal with xenotropic murine leukemia virus. | virus filtration (vf) is a key step in an overall viral clearance process since it has been demonstrated to effectively clear a wide range of mammalian viruses with a log reduction value (lrv) > 4. the potential to achieve higher lrv from virus retentive filters has historically been examined using bacteriophage surrogates, which commonly demonstrated a potential of > 9 lrv when using high titer spikes (e.g. 10(10) pfu/ml). however, as the filter loading increases, one typically experiences sign ... | 2015 | 25395156 |
| first prospective comparison of genotypic vs phenotypic tropism assays in predicting virologic responses to maraviroc (mvc) in a phase 3 study: modern. | modern (a4001095) was the first prospective phase 3 study comparing genotype vs phenotype (trofile™) tropism assessments. | 2014 | 25394028 |
| robust rna silencing-mediated resistance to plum pox virus under variable abiotic and biotic conditions. | some abiotic and biotic conditions are known to have a negative impact on post-transcriptional gene silencing (ptgs), thus representing a potential concern for the production of stable engineered virus resistance traits. however, depending on the strategy followed to achieve ptgs of the transgene, different responses to external conditions can be expected. in the present study, we utilized the nicotiana benthamiana–plum pox virus (ppv) pathosystem to evaluate in detail the stability of intron-ha ... | 2014 | 25346969 |
| sharka: how do plants respond to plum pox virus infection? | plum pox virus (ppv), the causal agent of sharka disease, is one of the most studied plant viruses, and major advances in detection techniques, genome characterization and organization, gene expression, transmission, and the description of candidate genes involved in ppv resistance have been described. however, information concerning the plant response to ppv infection is very scarce. in this review, we provide an updated summary of the research carried out to date in order to elucidate how plan ... | 2015 | 25336685 |
| coding algorithms for identifying patients with cirrhosis and hepatitis b or c virus using administrative data. | despite the use of administrative data to perform epidemiological and cost-effectiveness research on patients with hepatitis b or c virus (hbv, hcv), there are no data outside of the veterans health administration validating whether international classification of disease, ninth revision, clinical modification (icd-9-cm) codes can accurately identify cirrhotic patients with hbv or hcv. the validation of such algorithms is necessary for future epidemiological studies. | 2015 | 25335773 |
| evaluating a surveillance system: live-bird market surveillance for highly pathogenic avian influenza, a case study. | highly pathogenic avian influenza h5n1 was first reported in poultry in nigeria in february 2006. the only human case that occurred was linked to contact with poultry in a live bird market (lbm). lbm surveillance was instituted to assess the degree of threat of human exposure to h5n1. the key indicator was detection of h5n1 in lbms. we evaluated the surveillance system to assess its operations and attributes. | 2014 | 25328630 |
| performance of rapid influenza diagnostic testing in outbreak settings. | rapid influenza diagnostic tests (ridts) may be useful during institutional respiratory disease outbreaks to identify influenza and enable antivirals to be rapidly administered to patients and for the prophylactic treatment of those exposed to the virus but not yet symptomatic. the performance of ridts at the outbreak level is not well documented in the literature. this study aimed to evaluate the performance of ridts in comparison with that of real-time reverse transcription (rrt)-pcr in the co ... | 2014 | 25320225 |
| concordance of sustained virological response 4, 12, and 24 weeks post-treatment with sofosbuvir-containing regimens for hepatitis c virus. | historically, clinical trials of regimens to treat chronic infection with hepatitis c virus (hcv) have used, as their primary efficacy endpoint, a sustained virological response (svr)—defined as hcv rna levels below a designated threshold of quantification—24 weeks after the end of treatment (svr24). more recently, regulatory authorities have begun to accept svr at 12 weeks post-treatment (svr12) as a valid efficacy endpoint because of its high rate of concordance with svr24. however, the concor ... | 2015 | 25314116 |
| ion-channel genosensor for the detection of specific dna sequences derived from plum pox virus in plant extracts. | a dna biosensor for detection of specific oligonucleotides sequences of plum pox virus (ppv) in plant extracts and buffer is proposed. the working principles of a genosensor are based on the ion-channel mechanism. the nh2-ssdna probe was deposited onto a glassy carbon electrode surface to form an amide bond between the carboxyl group of oxidized electrode surface and amino group from ssdna probe. the analytical signals generated as a result of hybridization were registered in osteryoung square w ... | 2014 | 25302809 |
| ns1 antigen capture elisa an effective method for diagnosis of early dengue infection - report of an outbreak at angul district, odisha, india. | an outbreak of dengue infection occurred in angul district of odisha in the month of august & september, 2011. the study was undertaken to detect ns1 antigen positivity among the study population, to compare igm capture elisa with ns1 antigen detection for diagnosis of dengue and to identify the predominant genotype of dengue virus responsible for the outbreak. | 2014 | 25302194 |
| adult vaccination disparities among foreign-born populations in the u.s., 2012. | foreign-born persons are considered at higher risk of undervaccination and exposure to many vaccine-preventable diseases. information on vaccination coverage among foreign-born populations is limited. | 2014 | 25300733 |
| resistance to plum pox virus strain c in arabidopsis thaliana and chenopodium foetidum involves genome-linked viral protein and other viral determinants and might depend on compatibility with host translation initiation factors. | research performed on model herbaceous hosts has been useful to unravel the molecular mechanisms that control viral infections. the most common plum pox virus (ppv) strains are able to infect nicotiana species as well as chenopodium and arabidopsis species. however, isolates belonging to strain c (ppv-c) that have been adapted to nicotiana spp. are not infectious either in chenopodium foetidum or in arabidopsis thaliana. in order to determine the mechanism underlying this interesting host-specif ... | 2014 | 25296116 |
| transient expression of the influenza a virus pb1-f2 protein using a plum pox virus-based vector in nicotiana benthamiana. | pb1-f2 protein of influenza a virus (iav) was cloned in a plum pox virus (ppv) genome-based vector and attempts to express it in biolistically transfected nicotiana benthamiana plants were performed. the vector-insert construct replicated in infected plants properly and was stable during repeated passage by mechanical inoculation, as demonstrated by disease symptoms and immunoblot detection of ppv capsid protein, while pb1-f2-specific band was more faint. we showed that it was due its low solubi ... | 2014 | 25283863 |
| association of interferon-γ-induced protein-10 serum levels with virological responses to peg-interferon-based therapy in hepatitis c virus genotype 1 or 2 chronically infected chinese patients. | interferon (ifn)-γ-induced protein-10 (ip-10) serum level has been shown be associated with viral response in chronic hepatitis c (chc) patients. however, little is known in chinese population. we determined ip-10 serum levels in chinese chc patients undergoing peg-ifn-based therapy. predictive role of ip-10 level for virological responses was accessed. | 2014 | 25263691 |
| varicella-zoster virus immunity among health care workers in catalonia. | to determine varicella-zoster virus (vzv) immunity among healthcare workers (hcws). cross-sectional study. | 2014 | 25219564 |
| complete genome sequences of two biologically distinct isolates of asparagus virus 1. | the complete genome sequences of two asparagus virus 1 (av-1) isolates differing in their ability to cause systemic infection in nicotiana benthamiana were determined. their genomes had 9,741 nucleotides excluding the 3'-terminal poly(a) tail, encoded a polyprotein of 3,112 amino acids, and shared 99.6 % nucleotide sequence identity. they differed at 37 nucleotide and 15 amino acid sequence positions (99.5 % identity) scattered over the polyprotein. the closest relatives of av-1 in amino acid se ... | 2015 | 25216774 |
| screening metagenomic data for viruses using the e-probe diagnostic nucleic acid assay. | next generation sequencing (ngs) is not used commonly in diagnostics, in part due to the large amount of time and computational power needed to identify the taxonomic origin of each sequence in a ngs data set. by using the unassembled ngs data sets as the target for searches, pathogen-specific sequences, termed e-probes, could be used as queries to enable detection of specific viruses or organisms in plant sample metagenomes. this method, designated e-probe diagnostic nucleic acid assay, first t ... | 2014 | 25207481 |
| establishment and application of a multiplex pcr for rapid and simultaneous detection of six viruses in swine. | a multiplex pcr assay was developed and evaluated subsequently for its effectiveness in simultaneously detecting mixed viral infections of swine. specific primers were designed and used for testing the six swine viruses: three dna viruses, including pseudorabies virus (prv), porcine parvovirus (ppv), and porcine circovirus type 2 (pcv2); three common rna viruses, including porcine reproductive and respiratory syndrome virus (prrsv), classical swine fever virus (csfv), and japanese encephalitis v ... | 2014 | 25116201 |
| evaluation of viral inactivation of pseudorabies virus, encephalomyocarditis virus, bovine viral diarrhea virus and porcine parvovirus in pancreatin of porcine origin. | pancreatin is a substance containing enzymes, principally amylase, lipase, and protease. it is obtained from bovine or porcine pancreas and used in the treatment of pancreatic endocrine insufficiency in humans. regulations and safety concerns mandate viral clearance (virus removal or inactivation) in biopharmaceuticals such as pancreatin. a virus validation study was performed to evaluate virus clearance achieved in the final step of drying under vacuum by testing a panel of four animal viruses: ... | 2014 | 25110118 |
| development of an evagreen-based multiplex real-time pcr assay with melting curve analysis for simultaneous detection and differentiation of six viral pathogens of porcine reproductive and respiratory disorder. | concurrent infection of pigs with two or more pathogens is common in pigs under intensive rearing conditions. porcine circovirus type 2 (pcv2), porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus (prrsv), classical swine fever virus (csfv), japanese encephalitis virus (jev) and pseudorabies virus (prv) are all associated with reproductive or respiratory disorders or both and can cause significant economic losses in pig production worldwide. an evagreen-based multiplex r ... | 2014 | 25102430 |
| the validation of the 2010 american association for the study of liver diseases guideline for the diagnosis of hepatocellular carcinoma in an endemic area. | hepatocellular carcinoma (hcc) diagnosis could be made with one typical imaging study in a cirrhotic liver by the guideline of the american association for the study of liver diseases (aasld) in 2010. patients with hepatitis b who may not have fully developed cirrhosis could be applied. we aim to retrospectively analyze and validate the diagnostic power of the 2010 guideline in an hcc endemic area (taiwan). | 2015 | 25092265 |
| prediction of spontaneous hbeag seroconversion in hbeag-positive chronic hepatitis b patients during the immune clearance phase. | spontaneous hepatitis b virus (hbv) e antigen (hbeag) seroconversion is associated with reduced risk of liver-related complications, but is poorly understood. in this study, 113 chronic hepatitis b patients in the immune active hbeag-positive phase were followed up for 76 weeks. based on the outcome of liver function, hbeag, hepatitis b viral e antibody (anti-hbe) and hbv dna at week 76, 18 patients were classified as spontaneous hbeag seroconversion group (group a) and 95 patients were classifi ... | 2014 | 25088043 |
| separation of porcine parvovirus from bovine serum albumin using peg-salt aqueous two-phase system. | vaccine production faces a challenge in adopting conventional downstream processing steps that can efficiently purify large viral particles. some major issues that plague vaccine purification are purity, potency, and quality. the industry currently considers 30% as an acceptable virus recovery for a vaccine purification process, including all downstream processes, whereas antibody recovery from cho cell culture is generally around 80-85%. a platform technology with an improved virus recovery wou ... | 2014 | 25086421 |
| classic nuclear localization signals and a novel nuclear localization motif are required for nuclear transport of porcine parvovirus capsid proteins. | nuclear targeting of capsid proteins (vps) is important for genome delivery and precedes assembly in the replication cycle of porcine parvovirus (ppv). clusters of basic amino acids, corresponding to potential nuclear localization signals (nls), were found only in the unique region of vp1 (vp1up, for vp1 unique part). of the five identified basic regions (br), three were important for nuclear localization of vp1up: br1 was a classic pat7 nls, and the combination of br4 and br5 was a classic bipa ... | 2014 | 25078698 |
| generation of recombinant porcine parvovirus virus-like particles in saccharomyces cerevisiae and development of virus-specific monoclonal antibodies. | porcine parvovirus (ppv) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets. the gene coding for the major capsid protein vp2 of ppv was amplified using viral nucleic acid extract from swine serum and inserted into yeast saccharomyces cerevisiae expression plasmid. recombinant ppv vp2 protein was efficiently expressed in yeast and purified using density gradient centrifugation. electron microscopy analysis of purified ppv vp2 protein revealed ... | 2014 | 25045718 |
| a sensitive multiplex real-time pcr panel for rapid diagnosis of viruses associated with porcine respiratory and reproductive disorders. | the objective of this study was to develop a multiplex real-time pcr panel using taqman probes for the detection and differentiation of porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus north american type (prrsv-na), pseudorabies virus (prv), classical swine fever virus (csfv), porcine parvovirus type 1 (ppv1) and japanese encephalitis virus (jev). specific primer and probe combinations for pcv2, prrsv, prv, csfv, ppv1 and jev were selected within the conserv ... | 2014 | 25044282 |
| virus infection of haptolina ericina and phaeocystis pouchetii implicates evolutionary conservation of programmed cell death induction in marine haptophyte-virus interactions. | the mechanisms by which phytoplankton cope with stressors in the marine environment are neither fully characterized nor understood. as viruses are the most abundant entities in the global ocean and represent a strong top-down regulator of phytoplankton abundance and diversity, we sought to characterize the cellular response of two marine haptophytes to virus infection in order to gain more knowledge about the nature and diversity of microalgal responses to this chronic biotic stressor. we infect ... | 2014 | 25013242 |
| rapid diagnostic detection of plum pox virus in prunus plants by isothermal amplifyrp(®) using reverse transcription-recombinase polymerase amplification. | plum pox virus (ppv) causes the most destructive viral disease known as plum pox or sharka disease in stone fruit trees. as an important regulated pathogen, detection of ppv is thus of critical importance to quarantine and eradication of the spreading disease. in this study, the innovative development of two amplifyrp(®) tests is reported for a rapid isothermal detection of ppv using reverse transcription-recombinase polymerase amplification. in an amplifyrp(®) test, all specific recombination a ... | 2014 | 25010790 |
| porcine parvovirus flocculation and removal in the presence of osmolytes. | viruses can be modified into viral vaccines or gene therapy vectors in order to treat acquired or genetic diseases. to satisfy the current market demand, an improvement in current vaccine manufacturing is needed. chromatography and nanofiltration are not suitable for all types of viruses. in this study, we propose to use virus flocculation with osmolytes, followed by microfiltration, as a potential virus purification process. we hypothesize that osmolytes strongly bind to water, thus leading to ... | 2014 | 25003646 |
| virus removal capacity at varying ionic strength during nanofiltration of alphanine® sd. | nanofiltration is incorporated into the manufacturing processes of many protein biopharmaceuticals to enhance safety by providing the capacity to retain pathogens while allowing protein drugs to pass through the filter. retention is mainly a function of size; however, the shape of the pathogen may also influence retention. the ability of the viresolve(®) pro nanofilter to remove different sized viruses during the manufacture of a coagulation factor ix (alphanine(®) sd) was studied at varying ion ... | 2014 | 24999080 |
| analysis of gene expression changes in peach leaves in response to plum pox virus infection using rna-seq. | differences in gene expression were studied after plum pox virus (ppv, sharka disease) infection in peach gf305 leaves with and without sharka symptoms using rna-seq. for each sample, more than 80% of 100-nucleotide paired-end (pe) illumina reads were aligned on the peach reference genome. in the symptomatic sample, a significant proportion of reads were mapped to ppv reference genomes (1.04% compared with 0.00002% in non-symptomatic leaves), allowing for the ultra-deep assembly of the complete ... | 2015 | 24989162 |
| adsorption of a non-enveloped mammalian virus to functionalized nanofibers. | in the pursuit of finding superior methods to remove pathogens from drinking water, this study examines the adsorption of a non-enveloped, mammalian virus to highly charged nanofibers. n-[(2-hydroxyl-3-trimethylammonium) propyl] chitosan (htcc) nanofibers were synthesized by the addition of a quaternary amine to chitosan. htcc was blended with polyvinyl alcohol (pva) to produce nanofibers by electrospinning. the nanofibers were stabilized against water by crosslinking with glutaraldehyde. when s ... | 2014 | 24985761 |
| differential rnai responses of nicotiana benthamiana individuals transformed with a hairpin-inducing construct during plum pox virus challenge. | gene silencing and large-scale small rna analysis can be used to develop rna interference (rnai)-based resistance strategies for plum pox virus (ppv), a high impact disease of prunus spp. in this study, a pppvirna hairpin-inducing vector harboring two silencing motif-rich regions of the ppv coat protein (cp) gene was evaluated in transgenic nicotiana benthamiana (nb) plants. wild-type nb plants infected with a chimeric ppv virus (ppv::gfp) exhibited affected leaves with mosaic chlorosis congruen ... | 2014 | 24964777 |
| transcriptomic analysis of prunus domestica undergoing hypersensitive response to plum pox virus infection. | plum pox virus (ppv) infects prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. one variety of prunus domestica, named 'jojo', develops a hypersensitive response to viral infection. here we compared infected and non-infected samples using next-generation rna sequencing to characterize the genetic complexity of the viral population in infected samples and to identify genes involved in development of the resistance response. analysis of vira ... | 2014 | 24959894 |
| diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis b virus detection in the routine clinical diagnostic laboratory. | hepatitis b virus (hbv) is the most common significant chronic viral infection world-wide. hepatitis b surface antigen (hbsag) has been the principal target for laboratory testing to identify active infection by hbv. we aimed to find out diagnostic validity of the liaison chemiluminescent method compared to the polymerase chain reaction (pcr) method for hbv detection in the routine clinical diagnostic laboratory. | 2014 | 24949287 |
| a novel role of the potyviral helper component proteinase contributes to enhance the yield of viral particles. | the helper component proteinase (hcpro) is an indispensable, multifunctional protein of members of the genus potyvirus and other viruses of the family potyviridae. this viral factor is directly involved in diverse steps of viral infection, such as aphid transmission, polyprotein processing, and suppression of host antiviral rna silencing. in this paper, we show that although a chimeric virus based on the potyvirus plum pox virus lacking hcpro, which was replaced by a heterologous silencing suppr ... | 2014 | 24942578 |
| virus-induced gene silencing in transgenic plants: transgene silencing and reactivation associate with two patterns of transgene body methylation. | we used bisulfite sequencing to study the methylation of a viral transgene whose expression was silenced upon plum pox virus infection of the transgenic plant and its subsequent recovery as a consequence of so-called virus-induced gene silencing (vigs). vigs was associated with a general increase in the accumulation of small rnas corresponding to the coding region of the viral transgene. after vigs, the transgene promoter was not methylated and the coding region showed uneven methylation, with t ... | 2014 | 24916614 |
| clinical application evaluation of two fourth-generation human immunodeficiency virus (hiv) screening assays in west china hospital. | fourth-generation human immunodeficiency virus (hiv) screening assays have been used in many laboratories. the elecsys® hiv combi pt assay is a new kind of fourth-generation hiv screening assay developed to allow earlier detection of seroconversion. | 2015 | 24797498 |
| use of laboratory reports as predictors of west nile virus disease cases, texas, 2008-2012. | we evaluated laboratory reports as early indicators of west nile virus (wnv) disease cases in texas. we compared wnv laboratory results in the national electronic disease surveillance system base system (nbs) to wnv disease cases reported to the state health department from 2008 to 2012. we calculated sensitivity and positive predictive value (ppv) of nbs reports, estimated the number of disease cases expected per laboratory report, and determined lead and lag times. the sensitivity and ppv of n ... | 2015 | 24762928 |
| a comparison of two visual inspection methods for cervical cancer screening among hiv-infected women in kenya. | to determine the optimal strategy for cervical cancer screening in women with human immunodeficiency virus (hiv) infection by comparing two strategies: visual inspection of the cervix with acetic acid (via) and via followed immediately by visual inspection with lugol's iodine (via/vili) in women with a positive via result. | 2014 | 24700979 |
| cloning, expression and evaluation of diagnostic potential of recombinant capsid protein based igm elisa for chikungunya virus. | the resurgence of chikungunya virus in the form of unprecedented explosive epidemic with unusual clinical severity after a gap of 32 years is a point of major public health concern. definitive diagnosis is critical in differentiating the disease, especially in dengue endemic areas. the immunoglobulin m (igm) enzyme-linked immunosorbent assay (elisa) is widely used for diagnosis of chikungunya infection. however igm elisa based on whole virus antigen is associated with biohazard risk. the present ... | 2014 | 24681089 |
| utility of total lymphocyte count as a surrogate for absolute cd4 count in the adult indian hiv population: a prospective study. | standard methods of cd4 counts and plasma viral load estimation require specialized equipment, highly trained personnel and are extremely expensive. this remains a major challenge for the initiation of anti-retroviral therapy for patients in resource-limited settings. | 2014 | 24678463 |
| individual detection of 14 high risk human papilloma virus genotypes by the paptype test for the prediction of high grade cervical lesions. | hr hpv genotypes when assayed collectively, achieve high sensitivity but low specificity for the prediction of cin2+. knowledge of the specific genotypes in an infection may facilitate the use of hr hpv detection in routine clinical practice. | 2014 | 24630483 |
| performance and comparison of self-reported sti symptoms among high-risk populations - msm, sex workers, persons living with hiv/aids - in el salvador. | resource-limited countries have limited laboratory capability and rely on syndromic management to diagnose sexually transmitted infections (stis). we aimed to estimate the sensitivity, specificity and positive predictive value (ppv) of sti syndromic management when used as a screening method within a study setting. men who have sex with men (msm), female sex workers (fsws) and people living with hiv/aids (plwha) participated in a behavioural surveillance study. data were obtained on demographics ... | 2014 | 24616119 |
| antiviral effect of diammonium glycyrrhizinate on cell infection by porcine parvovirus. | porcine parvovirus (ppv) can cause reproductive failure in swine, resulting in economic losses to the industry. antiviral effects of diammonium glycyrrhizinate (dg) have been reported on several animal viruses; however, to date it has yet to be tested on ppv. in this study, the antiviral activity of dg on swine testis (st) cell infection by ppv was investigated using an empirically determined, non-toxic concentration of dg and three different experimental designs: (1) pre-treatment of virus prio ... | 2014 | 24614970 |
| the hypervariable amino-terminus of p1 protease modulates potyviral replication and host defense responses. | the replication of many rna viruses involves the translation of polyproteins, whose processing by endopeptidases is a critical step for the release of functional subunits. p1 is the first protease encoded in plant potyvirus genomes; once activated by an as-yet-unknown host factor, it acts in cis on its own c-terminal end, hydrolyzing the p1-hcpro junction. earlier research suggests that p1 cooperates with hcpro to inhibit host rna silencing defenses. using plum pox virus as a model, we show that ... | 2014 | 24603811 |
| arabidopsis protein phosphatase dbp1 nucleates a protein network with a role in regulating plant defense. | arabidopsis thaliana dbp1 belongs to the plant-specific family of dna-binding protein phosphatases. although recently identified as a novel host factor mediating susceptibility to potyvirus, little is known about dbp1 targets and partners and the molecular mechanisms underlying its function. analyzing changes in the phosphoproteome of a loss-of-function dbp1 mutant enabled the identification of 14-3-3λ isoform (grf6), a previously reported dbp1 interactor, and map kinase (mapk) mpk11 as componen ... | 2014 | 24595057 |
| reduction of spiked porcine circovirus during the manufacture of a vero cell-derived vaccine. | porcine circovirus-1 (pcv1) was recently identified as a contaminant in live rotavirus vaccines, which was likely caused by contaminated porcine trypsin. the event triggered the development of new regulatory guidance on the use of porcine trypsin which shall ensure that cell lines and porcine trypsin in use are free from pcv1. in addition, manufacturing processes of biologicals other than live vaccines include virus clearance steps that may prevent and mitigate any potential virus contamination ... | 2014 | 24560672 |
| comparison of hepatitis c virus rna and antibody detection in dried blood spots and plasma specimens. | current diagnostic tests for hepatitis c virus (hcv) involve phlebotomy and serologic testing for hcv antibodies (anti-hcv) and rna, which are not always feasible. dried blood spots (dbs) present a minimally invasive sampling method and are suitable for sample collection, storage and testing. | 2014 | 24529844 |
| trans-oral brush biopsies and quantitative pcr for ebv dna detection and screening of nasopharyngeal carcinoma. | to evaluate a newly developed noninvasive ambulatory, quantitative polymerase chain reaction (q-pcr) epstein-barr virus (ebv) dna detection and screening system (np screen™) for nasopharyngeal carcinoma (npc). | 2014 | 24486777 |
| comparison between screening and confirmatory serological assays in blood donors in a region of south italy. | screening assays are needed in order to guarantee safety of donated blood, but a significant number of safe donations are removed from blood supply because of reactive screening results. it is important to evaluate the positive predictive value (ppv) of screening assays in order to modulate confirmatory algorithm and implement an adequate counseling. | 2014 | 24478048 |
| peste des petits ruminants virus, mauritania. | 2014 | 24447781 | |
| identification of past and recent parvovirus b19 infection in immunocompetent individuals by quantitative pcr and enzyme immunoassays: a dual-laboratory study. | parvovirus b19 (b19v) is a member of the family parvoviridae, genus erythrovirus. b19v-specific igg and igm react differently against conformational and linear epitopes of vp1 and vp2 antigens, leading to the development of igg avidity and epitope type specificity (ets) enzyme immunoassays (eias) for distinguishing past from recent infection. additionally, b19v viral load determination (by quantitative pcr [qpcr]) is increasingly used in the staging of b19v infection. in this study, the utility ... | 2014 | 24403307 |
| comparative and retrospective molecular analysis of parapoxvirus (ppv) isolates. | species members of the genus parapoxvirus (ppv) within the family poxviridae cause contagious pustular dermatitis in small ruminants (orf virus, orfv) and mostly mild localized inflammation in cattle (bovine papular stomatitis virus, bpsv and pseudocowpox virus, pcpv). all ppvs are known to be zoonotic, leading to circumscribed skin lesions in humans, historically known as milker's nodules. human ppv isolates are often ill defined concerning their allocation to an animal origin. here we present ... | 2014 | 24373950 |
| differential replication of two porcine parvovirus strains in bovine cell lines ensues from initial dna processing and ns1 expression. | porcine parvovirus (ppv) is a small dna virus with restricted coding capacity. the 5 kb genome expresses three major non-structural proteins (ns1, ns2 and sat), and two structural proteins (vp1 and vp2). these few viral proteins are pleiotropic and interact with cellular components throughout viral replication. in this regard, very few cell lines have been shown to replicate the virus efficiently. cell lines were established from a primary culture of bovine cells that allowed allotropic variants ... | 2014 | 24347493 |
| comparative evaluation of the bio-rad geenius hiv-1/2 confirmatory assay and the bio-rad multispot hiv-1/2 rapid test as an alternative differentiation assay for clsi m53 algorithm-i. | the clsi-m53-a, criteria for laboratory testing and diagnosis of human immunodeficiency virus (hiv) infection; approved guideline includes an algorithm in which samples that are reactive on a 4th generation eia screen proceed to a supplemental assay that is able to confirm and differentiate between antibodies to hiv-1 and hiv-2. the recently ce-marked bio-rad geenius hiv-1/2 confirmatory assay was evaluated as an alternative to the fda-approved bio-rad multispot hiv-1/hiv-2 rapid test which has ... | 2013 | 24342484 |
| [value of high risk human papilloma virus detection in screening and diagnosing cervical lesion in littoral of zhejiang province]. | to analyse the infection of high-risk human papiliomavirus (hr-hpv) in cervical lesion wome, and evaluate the significance of high-risk human pappilomavirus detection by hybrid capture ii (hv-ii) in screening and diagnosing cervical lesion, especially high grade cervical intraepithelial neoplasia (cin). | 2013 | 24319961 |
| the role of genetic polymorphisms in cytochrome p450 and effects of tuberculosis co-treatment on the predictive value of cyp2b6 snps and on efavirenz plasma levels in adult hiv patients. | efavirenz (efv) exhibits interindividual pharmacokinetic variability caused by differences in cytochrome p450 (cyp) expression. most tuberculosis (tb) drugs interact with the cyp metabolizing enzymes, while the clinical validity of genotyping in predicting efv plasma levels in rwandan subjects is not known. we investigated in patients co-infected with human immunodeficiency virus (hiv) and tb recruited in rwanda the effects of 10 snps in five drug-metabolizing enzymes on efv plasma levels and tr ... | 2014 | 24316028 |
| coat protein mediated resistance to plum pox virus in nicotiana clevelandii and n. benthamiana. | transgenic nicotiana benthamiana and n. clevelandii plants expressing the coat protein of plum pox virus under the control of the 35s promoter from cauliflower mosaic virus were engineered by agrobacterium tumefaciens mediated transformation. the phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein and control plants were compared after challenge infection with plum pox virus. n. clevelandii coat protein transgenic plants circumvent ... | 1992 | 24213033 |
| regeneration of transgenic plants of prunus armeniaca containing the coat protein gene of plum pox virus. | a system was developed which allows the transfer of foreign genes into apricot cultivars. we report the transformation and regeneration of prunus armeniaca plants with agrobacterium tumefaciens strain lba 4404 containing various binary plasmids, pbingusint, carrying the marker gene ß-glucuronidase (gus) and pbinppvm, carrying the coat protein gene of plum pox virus (ppv). the marker gene gus was used for optical evaluation of the efficiency of the transformation system. the coat protein gene of ... | 1992 | 24213032 |
| predictive value of interferon-gamma release assays for postpartum active tuberculosis in hiv-1-infected women. | data on the prognostic utility of interferon-gamma release assays (igras) for active tuberculosis (tb) among human immunodeficiency virus 1 (hiv-1) infected individuals are limited. | 2013 | 24200267 |
| single amino acid changes in the 6k1-ci region can promote the alternative adaptation of prunus- and nicotiana-propagated plum pox virus c isolates to either host. | plum pox virus (ppv) c is one of the less common ppv strains and specifically infects cherry trees in nature. making use of two ppv-c isolates that display different pathogenicity features, i.e., swcmp, which had been adapted to nicotiana species, and by101, which had been isolated from cherry rootstock l2 (prunus lannesiana) and propagated only in cherry species, we have generated two infective full-length cdna clones in order to determine which viral factors are involved in the adaptation to e ... | 2014 | 24200075 |
| transgenic plums (prunus domestica l.) express the plum pox virus coat protein gene. | plum hypocotyl slices were transformed with the coat protein (cp) gene of plum pox virus (ppv-cp) following cocultivation with agrobacterium tumefaciens containing the plasmid pga482gg/ppvcp-33. this binary vector carries the ppv-cp gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidase genes. integration and expression of the transferred genes into regenerated plum plants was verified through kan resistance, gus assays, and pcr amplification of the ppv-cp gene. ... | 1994 | 24194220 |
| assessment of non-invasive models for liver fibrosis in chronic hepatitis b virus related liver disease patients in resource limited settings. | a total of 350 million individuals are affected by chronic hepatitis b virus infection world-wide. historically, liver biopsy has been instrumental in adequately assessing patients with chronic liver disease. a number of non-invasive models have been studied world-wide. | 2015 | 24152493 |
| clinical and laboratory predictive markers for acute dengue infection. | early diagnosis of dengue virus infection during the febrile stage is essential for adjusting appropriate management. this study is to identify the predictive markers of clinical and laboratory findings in the acute stage of dengue infection during a major outbreak of dengue virus type 1 that occurred in southern taiwan during 2007. a retrospective, hospital-based study was conducted at a university hospital in southern taiwan from january to december, 2007. patient who was reported for clinical ... | 2013 | 24138072 |
| a novel approach to achieving modular retrovirus clearance for a parvovirus filter. | viral filtration is routinely incorporated into the downstream purification processes for the production of biologics produced in mammalian cell cultures (mcc) to remove potential viral contaminants. in recent years, the use of retentive filters designed for retaining parvovirus (~20 nm) has become an industry standard in a conscious effort to further improve product safety. since retentive filters remove viruses primarily by the size exclusion mechanism, it is expected that filters designed for ... | 2014 | 24123923 |
| a sensitive and specific nanoparticle-assisted pcr assay for rapid detection of porcine parvovirus. | a novel nanoparticle-assisted polymerase chain reaction (nanopcr) assay to detect porcine parvovirus (ppv) is described here. primers for this assay were designed based on the conserved region of the nonstructural protein 1 (ns1) gene of ppv, which encodes one of the nonstructural proteins. the sensitivity of the ppv nanopcr assay was measured by using diluted recombinant plasmids in which the ppv ns1 gene had been inserted. the detection limit was 5.6 × 10(1) copies μl(-1) for the ppv nanopcr a ... | 2014 | 24117951 |
| body mass index and liver stiffness affect accuracy of ultrasonography in detecting steatosis in patients with chronic hepatitis c virus genotype 1 infection. | few studies have evaluated the accuracy of ultrasonography in detecting steatosis in patients with chronic hepatitis c. we assessed its accuracy in detecting steatosis and factors that affect its diagnostic performance in consecutive patients with chronic hepatitis c virus genotype 1 infection. | 2014 | 24112998 |
| plum pox virus and sharka: a model potyvirus and a major disease. | plum pox virus (ppv) is a member of the genus potyvirus in the family potyviridae. ppv diversity is structured into at least eight monophyletic strains. | 2014 | 24102673 |
| epidemiology of viral respiratory tract infections in an outpatient haematology facility. | viral respiratory tract infections (vrti) are an important cause of morbidity and mortality in haematology patients, particularly after haematopoietic stem cell transplantation (hsct). the incidence, clinical presentation and outcome of symptomatic and asymptomatic vrti in hsct outpatient unit were prospectively evaluated during a single influenza season (january-march 2011). pharyngeal swabs were performed at the first visit and if new symptoms were present. molecular multiplex assay for 12 res ... | 2014 | 24097084 |
| fluctuating temperature leads to evolution of thermal generalism and preadaptation to novel environments. | environmental fluctuations can select for generalism, which is also hypothesized to increase organisms' ability to invade novel environments. here, we show that across a range of temperatures, opportunistic bacterial pathogen serratia marcescens that evolved in fluctuating temperature (daily variation between 24°c and 38°c, mean 31°c) outperforms the strains that evolved in constant temperature (31°c). the growth advantage was also evident in novel environments in the presence of parasitic virus ... | 2013 | 24094344 |
| non-enveloped virus reduction with quaternized chitosan nanofibers containing graphene. | membranes are an accepted technology for water purification. membrane filtration can remove pathogens, including bacteria and viruses, by size. for small viruses that can have a diameter <25nm, removal by size leads to large membrane areas, high transmembrane pressures, low water flux, and frequent changing of membranes. in this work, we discovered that electrospun nanofibers made of chitosan and functionalized with a quaternary amine (htcc) have the ability to adsorb a model non-enveloped virus ... | 2013 | 24041631 |