Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| wild birds as reservoirs for diverse and abundant gamma- and deltacoronaviruses. | wild birds interconnect all parts of the globe through annual cycles of migration with little respect for country or continental borders. although wild birds are reservoir hosts for a high diversity of gamma- and deltacoronaviruses, we have little understanding of the ecology or evolution of any of these viruses. in this review, we use genome sequence and ecological data to disentangle the evolution of coronaviruses in wild birds. specifically, we explore host range at the levels of viral genus ... | 2020 | 32672814 |
| a novel mucosal adjuvant system for immunization against avian coronavirus causing infectious bronchitis. | infectious bronchitis (ib) caused by infectious bronchitis virus (ibv) is currently a major threat to chicken health, with multiple outbreaks being reported in the united states over the past decade. modified live virus (mlv) vaccines used in the field can persist and provide the genetic material needed for recombination and emergence of novel ibv serotypes. inactivated and subunit vaccines overcome some of the limitations of mlv with no risk of virulence reversion and emergence of new virulent ... | 2020 | 32669327 |
| pathogenic characteristics of a qx-like infectious bronchitis virus strain sd in chickens exposed at different ages and protective efficacy of combining live homologous and heterologous vaccination. | continued reports of infections with infectious bronchitis virus (ibv) variants have occurred since its first isolation in the 1930s. currently, qx-like ibvs are the predominant circulating genotype around the world. here, the pathogenicity of qx-like ibv strain sd was characterized in chickens at different ages of exposure to the virus, and the protection efficacy of available vaccine combinations against ibv was evaluated. the results revealed that qx-like ibv strain sd was severely pathogenic ... | 2020 | 32641162 |
| emerging infectious bronchitis virus (ibv) in egypt: evidence for an evolutionary advantage of a new s1 variant with a unique gene 3ab constellation. | infectious bronchitis virus (ibv), a gamma-coronavirus, causes infectious bronchitis (ib), a major respiratory disease of chicken. its high mutation rate in conjunction with recombination of the rna genome constantly creates ibv variants that are difficult to control by currently available vaccines. in this study, we addressed the question whether small-scale holdings might harbor ibv variants that serve as a reservoir for newly emerging variants. egyptian ibv isolate egy/nr725/2016 (nr725/16) f ... | 2020 | 32622080 |
| molecular monitoring of d1466 genotype of avian infectious bronchitis virus in iran: a retrospective study in 2013-2017. | infectious bronchitis is one of the most common diseases in the poultry industry in many countries, especially in the regions with a dense poultry farming industry. gammacoronavirus is the etiologic agent of this disease, with the chickens and poultries as the natural reservoirs of the virus. various strains of infectious bronchitis virus have been reported in poultry around the world. in terms of pathogenicity, this virus can induce a spectrum of diseases ranging from the moderate respiratory t ... | 2020 | 32621444 |
| molecular detection of avian infectious bronchitis viruses in live bird markets, gilan province. | coronaviruses (avcov) which include infectious bronchitis virus (ibv) and other bird coronaviruses belong to the genus gammacoronavirus, subfamily coronavirinae. one of the most prominent representatives of gammacoronavirus genus is infectious bronchitis virus (ibv) which is a highly contagious viral pathogen of chickens causing considerable economic losses to the poultry industry. ibvs mostly affect the respiratory, urinary, and reproductive tracts leading to a substantial drop in production. b ... | 2020 | 32621443 |
| comparative protective efficacies of novel avian paramyxovirus-vectored vaccines against virulent infectious bronchitis virus in chickens. | viral vectored vaccines are desirable alternatives for conventional infectious bronchitis virus (ibv) vaccines. we have recently shown that a recombinant newcastle disease virus (rndv) strain lasota expressing the spike (s) protein of ibv strain mass-41 (rlasota/ibv-s) was a promising vaccine candidate for ibv. here we evaluated a novel chimeric rndv/avian paramyxovirus serotype 2 (rndv/apmv-2) as a vaccine vector against ibv. the rndv/apmv-2 vector was chosen because it is much safer than the r ... | 2020 | 32605292 |
| nitric oxide production and fc receptor-mediated phagocytosis as functional readouts of macrophage activity upon stimulation with inactivated poultry vaccines in vitro. | vaccine batches must pass routine quality control to confirm that their ability to induce protection against disease is consistent with batches of proven efficacy from development studies. for poultry vaccines, these tests are often performed in laboratory chickens by vaccination-challenge trials or serological assays. the aim of this study was to investigate innate immune responses against inactivated poultry vaccines and identify candidate immune parameters for in vitro quality tests as altern ... | 2020 | 32580391 |
| infectious bronchitis virus evolution, diagnosis and control. | rna viruses are characterized by high mutation and recombination rates, which allow a rapid adaptation to new environments. most of the emerging diseases and host jumps are therefore sustained by these viruses. rapid evolution may also hinder the understanding of molecular epidemiology, affect the sensitivity of diagnostic assays, limit the vaccine efficacy and favor episodes of immune escape, thus significantly complicating the control of even well-known pathogens. the history of infectious bro ... | 2020 | 32580381 |
| limited cross-protection against infectious bronchitis provided by recombinant infectious bronchitis viruses expressing heterologous spike glycoproteins. | gammacoronavirus infectious bronchitis virus (ibv) causes an economically important respiratory disease of poultry. protective immunity is associated with the major structural protein, spike (s) glycoprotein, which induces neutralising antibodies and defines the serotype. cross-protective immunity between serotypes is limited and can be difficult to predict. in this study, the ability of two recombinant ibv vaccine candidates, beaur-m41(s) and beaur-4/91(s), to induce cross-protection against a ... | 2020 | 32580371 |
| genetic, antigenic, and pathogenic characteristics of infectious bronchitis virus gi-7/tw-ii in china. | nine infectious bronchitis virus (ibv) strains belonging to the gi-7 lineage were isolated between 2009 and 2017 in china. phylogenetic analysis and comparisons of full-length sequences of the s1 gene suggested that the gi-7 lineage should be further classified as taiwan (tw)-i and tw-ii sublineages, which correspond to the previous tw-i and tw-ii genotypes. the nine ibv strains were clustered in the tw-ii sublineage. further investigation revealed that viruses in the tw-i and tw-ii were not onl ... | 2020 | 32550619 |
| infectious coryza: persistence, genotyping, and vaccine testing. | the reemergence of infectious coryza (ic) caused by avibacterium paragallinarum (ap) as an acute and occasionally chronic respiratory disease in domestic poultry has caused severe losses in several u.s. states. the disease is also associated with decreased egg production in layers and increased condemnations from air sac infections in broilers. a series of applied experiments were performed to elucidate the persistence of ap in infected broiler flocks, to genotype ap strains isolated from field ... | 2020 | 32550616 |
| diagnostic accuracy of ultrasonography to detect false layers in a commercial laying flock infected by an infectious bronchitis virus delmarva genotype causing cystic oviducts. | infection of the oviduct by an infectious bronchitis virus (ibv) in laying hens has been associated with the false layer syndrome. because the diagnostic procedure for the detection of cystic oviducts by postmortem examinations in ibv-positive replacement pullet flocks could involve the unnecessary sacrifice of numerous healthy pullets without reproductive tract anomalies, the development of a noninvasive and nonlethal diagnostic procedure would be desirable. the first objective of the study was ... | 2020 | 32550615 |
| health status of free-ranging ring-necked pheasant chicks (phasianus colchicus) in north-western germany. | being a typical ground-breeding bird of the agricultural landscape in germany, the pheasant has experienced a strong and persistent population decline with a hitherto unexplained cause. contributing factors to the ongoing negative trend, such as the effects of pesticides, diseases, predation, increase in traffic and reduced fallow periods, are currently being controversially discussed. in the present study, 62 free-ranging pheasant chicks were caught within a two-year period in three federal sta ... | 2020 | 32544211 |
| infectious bronchitis virus inhibits activation of the tlr7 pathway, but not the tlr3 pathway. | various strains of infectious bronchitis virus (ibv) cause different forms of infectious bronchitis with different clinical signs. here, primary chicken embryo kidney (cek) cells and specific-pathogen-free (spf) chickens were infected with three pathogenic ibv strains, and it was observed that the tlr7-myd88 pathway was inhibited but the tlr3-tirf pathway was activated. after treatment with poly(i:c)-lmw, poly (i:c)-lmw/lyovec, and imiquimod, the replication of ibv was significantly suppressed a ... | 2020 | 32524263 |
| efficacy of infectious bronchitis gi-13 (793b) vaccine candidate tested according to the current european union requirements and for cross-protection against heterologous qx-like challenge. | infectious bronchitis (ib) is a highly contagious viral disease of chickens, known to cause severe economic losses. vaccination against ib virus (ibv) is an important control measure against the disease. the objective of the present study was to test avishield ib gi-13, the vaccine candidate against ibv, strain v-173/11 (gi-13 genotype), according to european pharmacopoeia (ph. eur.) efficacy requirements. laboratory study on specific-pathogen-free (spf) chickens showed 100% protection against c ... | 2020 | 32522104 |
| the construction and immunoadjuvant activities of the oral interleukin-17b expressed by lactobacillus plantarum nc8 strain in the infectious bronchitis virus vaccination of chickens. | interleukin-17b (il-17b) is a protective cytokine of the il-17 family and plays an essential role in the regulation of mucosal inflammation. however, little is known about the role of il-17b in the control of viral infections. in this study, a recombinant lactobacillus plantarum, designated as nc8-chil17b, was constructed to express the chicken il-17b (chil-17b) gene. the recombinant chil17b (rchil17b) protein was about 14 kda and was anchored to the surface of nc8 cells. in vitro, it was found ... | 2020 | 32517220 |
| changes in the percentages of b- and t-lymphocytes and antibody titres in laying hens infested with dermanyssus gallinae-a preliminary study. | (1) background: dermanyssus gallinae, a hematophagous ectoparasite, adversely affects the health status of laying hens, leading to reduced egg production and significant economic losses in commercial farms. the aim of this study was to determine the effect of d. gallinae on the development of post-vaccination immune responses in layer hens. (2) methods: a total of 80 blood samples were collected at four time-points (b1-b4) from 10 hy-line brown hens, randomly selected from a commercial layer far ... | 2020 | 32517104 |
| a unifying structural and functional model of the coronavirus replication organelle: tracking down rna synthesis. | zoonotic coronavirus (cov) infections, such as those responsible for the current severe acute respiratory syndrome-cov 2 (sars-cov-2) pandemic, cause grave international public health concern. in infected cells, the cov rna-synthesizing machinery associates with modified endoplasmic reticulum membranes that are transformed into the viral replication organelle (ro). although double-membrane vesicles (dmvs) appear to be a pan-cov ro element, studies to date describe an assortment of additional cov ... | 2020 | 32511245 |
| evaluation of inactivated vaccine of the variant 2 (is-1494 /gi-23) genotype of avian infectious bronchitis. | the infectious bronchitis virus (ibv) is the cause of avian infectious bronchitis (ib). ib is one of the most highly contagious diseases, which results in many economic losses in the poultry industry worldwide. the nature of this virus is such that it generates new genotypes continuously. proper vaccination is the most suitable way of combatting ib. one of the novel genotypes of ibv, which has been circulating in the middle eastern countries, is the variant 2 (is-1494/gi-23) genotype. this study ... | 2020 | 32505764 |
| induction of robust immune responses by cpg-odn-loaded hollow polymeric nanoparticles for antiviral and vaccine applications in chickens. | poultry vaccine has limited choices of adjuvants and is facing severe threat of infectious diseases due to ineffective of widely used commercial vaccines. thus, development of novel adjuvant that offers safe and effective immunity is of urgent need. | 2020 | 32494131 |
| evolutionary analysis of infectious bronchitis virus reveals marked genetic diversity and recombination events. | in the last 5 years, frequent outbreaks of infectious bronchitis virus (ibv) are observed in both broiler and layer chicken flocks in the kingdom of saudi arabia (ksa) in spite of extensive usage of vaccines. the ibv is a widespread avian coronavirus affecting both vaccinated and unvaccinated chicken flocks and is attributed to significant economic losses, around the globe. in the present study, 58 (n = 58) samples were collected from four different commercial poultry flocks from 8 ksa districts ... | 2020 | 32486006 |
| epidemiological investigation of avian infectious bronchitis and locally determined genotype diversity in central china: a 2016-2018 study. | infectious bronchitis (ib), caused by avian ib virus (ibv), is an acute and highly contagious disease of chickens. from 2016 to 2018, 56 ibv strains were isolated and identified from clinical samples obtained from various chicken farms located in central china. the s1 sequencing of these strains revealed nucleotide and amino acid identities of 70.2 to 100% and 62.6 to 100%, respectively, compared with those of reference strains. phylogenetic analysis indicated that the genotypes of the isolates ... | 2020 | 32475435 |
| molecular and pathogenicity of infectious bronchitis virus (gammacoronavirus) in japanese quail (coturnix japonica). | infectious bronchitis virus (ibv) infection is highly infectious respiratory disease in poultry industry with significant economic importance. the prevalence of ibv in quail industry in malaysia was not well documented; therefore, its actual role in the epidemiology of the disease is relatively unknown. this study was to determine the susceptibility of japanese quail, as one of the species in commercial poultry industry, toward ibv. in addition, it will also give a potential impact on the overal ... | 2020 | 32475428 |
| adjuvant effects of platycodin d on immune responses to infectious bronchitis vaccine in chickens. | adjuvants are common vaccine components. novel adjuvants may improve the protective immunity conferred by vaccines against poultry diseases. here, a less-hemolytic saponin, platycodin d (pd), isolated from the root of platycodon grandiflorum was investigated as a potential alternative adjuvant. pd was tested as an adjuvant in the infectious bronchitis (ib) vaccine, because the existing ib vaccine has often failed to induce effective immune responses. the adjuvant activity of pd in conjunction wi ... | 2020 | 32461731 |
| molecular characterization of infectious bronchitis virus based on rna-dependent rna polymerase gene. | extensive rate of variations in the s1 gene (spike glycoprotein subunit gene) of infectious bronchitis virus (ibv) causes challenges for clinicians in counting variants for differentiation of infected from vaccinated birds and addressing the variants of unknown significance. this study investigated the possibility of using an rna-dependent rna polymerase gene (rdrp) as a target for molecular characterization of ibv strains in iran. trachea samples were collected from commercial broiler flocks (n ... | 2020 | 32458483 |
| a novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in china. | at present, pig industry in china is faced with the complex situation of mixed infection caused by multiple pathogens. it is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies. biochip array technology has made it possible to screen thousands of samples simultaneously; it has been twice named as one of the top 10 scientific and technological breakthroughs. studies have reported encouraging results using protein biochips for dete ... | 2020 | 32456688 |
| characterization of novel, pathogenic field strains of infectious bronchitis virus (ibv) in poultry in trinidad and tobago. | avian coronaviruses, including infectious bronchitis virus (ibv) and turkey coronavirus (tcov), are economically important viruses affecting poultry worldwide. ibv is responsible for causing severe losses to the commercial poultry sector globally. the objectives of this study were to identify the viruses that were causing outbreaks of severe respiratory disease in chickens in trinidad and tobago (t&t) and to characterize the strains. swab samples were collected from birds showing severe respirat ... | 2020 | 32438523 |
| pustulan activates chicken bone marrow-derived dendritic cells in vitro and promotes ex vivo cd4+ t cell recall response to infectious bronchitis virus. | infectious bronchitis virus (ibv) is a highly contagious avian coronavirus. ibv causes substantial worldwide economic losses in the poultry industry. vaccination with live-attenuated viral vaccines, therefore, are of critical importance. live-attenuated viral vaccines, however, exhibit the potential for reversion to virulence and recombination with virulent field strains. therefore, alternatives such as subunit vaccines are needed together with the identification of suitable adjuvants, as subuni ... | 2020 | 32429204 |
| infectious bronchitis virus regulates cellular stress granule signaling. | viruses must hijack cellular translation machinery to express viral genes. in many cases, this is impeded by cellular stress responses. these stress responses result in the global inhibition of translation and the storage of stalled mrnas, into rna-protein aggregates called stress granules. this results in the translational silencing of the majority of mrnas excluding those beneficial for the cell to resolve the specific stress. for example, the expression of antiviral factors is maintained duri ... | 2020 | 32422883 |
| immunomodulatory effect of ginseng stem-leaf saponins and selenium on harderian gland in immunization of chickens to newcastle disease vaccine. | our previous study demonstrated that ginseng stem-leaf saponins (gsls) in combination with selenium (gsls-se) have adjuvant effect on the live vaccine of newcastle disease virus (ndv) and infectious bronchitis virus (ibv) in intraocular-and-intranasal immunization in chickens. the present study was to investigate the potential molecular mechanisms involved in the immunomodulation of gsls-se on the harderian gland (hg). it was found that the window allowing animals susceptible to infections due t ... | 2020 | 32422443 |
| reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus. | the study was conducted to develop a specific, simple, and sensitive method for diagnosis of avian infectious bronchitis virus (ibv). in this experiment, the selected downstream primer was labeled with biotin and the 5' end of raa probe was labeled with fam by reverse transcription recombinase-aided amplification (rt-raa) combined with lateral flow dipstick (lfd). a rt-raa-lfd assay that could be used for detection of ibv was established after optimization of rt-raa reaction time, reaction tempe ... | 2020 | 32416856 |
| pathogenicity of a qx-like avian infectious bronchitis virus isolated in china. | avian infectious bronchitis is a serious and highly contagious disease caused by infectious bronchitis virus (ibv). we isolated a highly virulent ibv strain (ck/ch/js/tahy) from kidneys of diseased chickens. phylogenetic analysis based on the s1 gene revealed that ck/ch/js/tahy clustered with the qx-like type. the s1 gene has 1,620 nucleotides and encoded a polypeptide of 540 amino acids with typical coronavirus cleavage recognition sites of hrrr. about 1-day-old specific pathogen-free white leg ... | 2020 | 32416792 |
| immunosensor based on antibody-functionalized mos2 for rapid detection of avian coronavirus on cotton thread. | infectious bronchitis virus (ibv), an avian coronavirus, significantly affects the performance of both the egg-laying and meat-type birds causing the foremost of economic loss in poultry industry. this paper aims to develop a rapid, low-cost, and sensitive biosensor for ibv detection by using molybdenum disulfide (mos2). mos2 is a 2-d nanosheet which has strong high fluorescence-quenching ability when applied to a dye-labeled antibody (ab). in this paper, we developed an ab-functionalized mos2-b ... | 2018 | 32390783 |
| a highly pathogenic gi-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism. | in the present study, an ibv strain i0305/19 was isolated from a diseased commercial broiler flock in 2019 in china with high morbidity and mortality. the isolate i0305/19 was clustered together with viruses in sublineage d of gi-19 lineage on the basis of the complete s1 sequence analysis. isolate i0305/19 and other gi-19 viruses isolated in china have the amino acid sequence mia at positions 110-112 in the s protein. further analysis based on the complete genomic sequence showed that the isola ... | 2020 | 32380209 |
| spotlight on avian coronaviruses. | coronaviruses (covs) mainly cause enteric and/or respiratory signs. mammalian covs including covid-19 (now officially named sars-cov-2) belong to either the alphacoronavirus or betacoronavirus genera. in birds, the majority of the known covs belong to the gammacoronavirus genus, whilst a small number are classified as deltacoronaviruses. gammacoronaviruses continue to be reported in an increasing number of avian species, generally by detection of viral rna. apart from infectious bronchitis virus ... | 2020 | 32374218 |
| emergence of variant avian infectious bronchitis virus in india. | infectious bronchitis virus (ibv) is the etiological agent of an acute and highly contagious disease. infectious bronchitis (ib) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. the continuous evolution of the spike protein (s1) of ibv is responsible for the prevalence of many serotypes/genotypes around the world. multiple lineages of ibv strains have been detected in chicken flocks in india since 2003. | 2020 | 32368223 |
| high-resolution melting curve analysis for infectious bronchitis virus strain differentiation. | belonging to the coronaviridae family, avian infectious bronchitis virus (ibv) causes respiratory, reproductive, and renal diseases in poultry. preventative measures lie mainly in vaccination, while the gold standard for ibv classification and differentiation is based on the sequence analysis of the spike 1 (s1) gene. in this study, we tested a new assay for ibv strain classification that is less expensive and requires reduced time and effort to perform. we carried out a quantitative real-time p ... | 2020 | 32367941 |
| natural product-derived phytochemicals as potential agents against coronaviruses: a review. | coronaviruses are responsible for a growing economic, social and mortality burden, as the causative agent of diseases such as severe acute respiratory syndrome (sars), middle east respiratory syndrome (mers), avian infectious bronchitis virus (ibv) and covid-19. however, there is a lack of effective antiviral agents for many coronavirus strains. naturally existing compounds provide a wealth of chemical diversity, including antiviral activity, and thus may have utility as therapeutic agents again ... | 2020 | 32360300 |
| development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus. | infectious laryngotracheitis disease is an acute, highly contagious viral disease seriously affecting poultry industry worldwide. in this study, a rapid and simple immune colloidal gold test strip for detecting infectious laryngotracheitis virus (iltv) was developed based on membrane chromatography with monoclonal antibodies (mabs) against gj protein of iltv and systematically evaluated for the detection of iltv from clinical samples. mab 2d4 1d7 was conjugated with colloidal gold as the detecto ... | 2020 | 32359575 |
| phylodynamic analysis and evaluation of the balance between anthropic and environmental factors affecting ibv spreading among italian poultry farms. | infectious bronchitis virus (ibv) control is mainly based on wide vaccine administration. although effective, its efficacy is not absolute, the viral circulation is not prevented and some side effects cannot be denied. despite this, the determinants of ibv epidemiology and the factors affecting its circulation are still largely unknown and poorly investigated. in the present study, 361 ibv qx (the most relevant field genotype in italy) sequences were obtained between 2012 and 2016 from the two m ... | 2020 | 32350378 |
| in ovo delivered toll-like receptor 7 ligand, resiquimod enhances host responses against infectious bronchitis corona virus (ibv) infection. | toll-like receptor (tlr) 7 ligand, resiquimod, has been studied as an adjuvant and antiviral agent against several pathogens in chicken. yet, the effectiveness of resiquimod against infectious bronchitis virus (ibv) infection has not been evaluated. in this study, we investigated the effectiveness of resiquimod delivered pre-hatch (in ovo) against ibv infection post-hatch identifying key mechanisms involved in resiquimod driven immune activation. first, we found an upregulation of interleukin (i ... | 2020 | 32326430 |
| decontamination of face masks with steam for mask reuse in fighting the pandemic covid-19: experimental supports. | the coronavirus disease 2019 pandemic caused by the novel coronavirus sars-cov-2 (severe acute respiratory syndrome coronavirus 2) has claimed many lives worldwide. wearing medical masks (mms) or n95 masks ([n95ms] namely n95 respirators) can slow the virus spread and reduce the infection risk. reuse of these masks can minimize waste, protect the environment, and help solve the current imminent shortage of masks. disinfection of used masks is needed for their reuse with safety, but improper deco ... | 2020 | 32320083 |
| cocirculation of four infectious bronchitis virus lineages in broiler chickens in the eastern region of saudi arabia from 2012 to 2014. | avian infectious bronchitis virus (ibv) is an evolving and dynamic virus that causes major economic losses for the poultry industry worldwide. continuous evolution and emergence of new variants of this virus are the major challenges for controlling the disease with routine vaccination. successful vaccination usually requires the use of a homologous vaccine, which in turn necessitates continuous investigation of the circulating strains. herein, we performed a reverse transcriptase-polymerase chai ... | 2020 | 32292579 |
| immunogenicity and efficacy of live infectious bronchitis 793/b.08ir vaccine in spf chickens. | infectious bronchitis virus (ibv) has a variety of serotypes with relatively limited cross-protection leading the disease to be a major problem in the poultry industry. the ibv 793/b strain has identified to circulate in iran; therefore, the development of a specific vaccine to protect against the virulent virus has received attention. in this regard, the live ib 793/b vaccine (793/b.08ir) was developed in the razi vaccine and serum research institute. in this study, the immunogenicity of 793/b. ... | 2020 | 32291999 |
| molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in egypt during 2012. | one of the major problems of avian infectious bronchitis virus (ibv) is the frequent emergence of new variants. in the present study 205 tracheal swabs and organs were collected from broilers and layers chicken farms during january to august 2012 from 19 governorates all over egypt. the chickens demonstrated respiratory signs and mortality. out of the examined samples, 130 of which (about 64%) of suspected farms were positive for ibv with real time rt-pcr. 13 ibv-positive samples were selected f ... | 2013 | 32289036 |
| a duplex rt-pcr assay for detection of h9 subtype avian influenza viruses and infectious bronchitis viruses. | h9 subtype avian influenza virus (aiv) and infectious bronchitis virus (ibv) are major pathogens circulating in poultry and have resulted in great economic losses due to respiratory disease and reduced egg production. as similar symptoms are elicited by the two pathogens, it is difficult for their differential diagnosis. so far, no reverse transcription-polymerase chain reaction (rt-pcr) assay has been found to differentiate between h9 aiv and ibv in one reaction. therefore, developing a sensiti ... | 2016 | 32288952 |
| current situation, genetic relationship and control measures of infectious bronchitis virus variants circulating in african regions. | infectious bronchitis virus (ibv) is a major viral pathogen of commercial poultry, affecting chickens of all ages and causing major economic losses in poultry industry worldwide. frequent points of mutations and recombination events in the s1 gene region, result in the emergence of new ibvs variants circulating in the form of several serotypes/genotypes that can be partially or poorly neutralized by current vaccines. ibv is well studied worldwide, nevertheless in african countries epidemiologica ... | 2016 | 32288944 |
| efficacy of intranasal and spray delivery of adjuvanted live vaccine against infectious bronchitis virus in experimentally infected poultry. | live vaccines are widely used in the avian industry. such vaccines can be either injected or delivered on animal mucosa and are usually not adjuvanted. in this study we show that live vaccines efficacy can be improved by formulation with adjuvants in a model of mucosal delivery of live infectious bronchitis vaccine in chicken. three adjuvant technologies have been tested using intranasal and spray delivery methods to poultry. those technologies are water in oil in water emulsion, nanoparticles a ... | 2012 | 32288918 |
| evaluation of protection conferred by a vaccination program based on the h120 and cr88 commercial vaccines against a field variant of avian infectious bronchitis virus. | due to serotype variations among different avian infectious bronchitis viruses isolated in tunisia since 2000, protection of chicks, especially broiler flocks, with mass h120 vaccine often fails. therefore, association of cr88 (793b type) with h120 vaccines was used for better response. challenge experiments were then conducted to evaluate tracheal and renal cross-protection in chickens immunized via nasal and eye drops. conferred protection was measured by clinical signs and macroscopic lesions ... | 2014 | 32288459 |
| new pa/1220/98-like variant of infectious bronchitis virus in poland. | the aim of the present study was to report the first detection of a new infectious bronchitis virus (ibv) variant in polish commercial flocks which is completely different to any previously known in this region. in 2018, samples from ross 308 breeding hens aged 35 weeks were delivered for ibv diagnosis. ibv presence was detected, but all attempts to amplify the s gene fragment were negative. the field material was analysed using the illumina miseq platform and a 1073-nt fragment of the s1 coding ... | 2020 | 32271091 |
| infectious bronchitis virus immune responses in the harderian gland upon initial vaccination. | in recent years, arkansas delmarva poultry industry (arkdpi)-derived infectious bronchitis (ib) virus (ibv) vaccines have been used to characterize the immune responses of chickens subsequent to vaccination on day of hatch or beyond. perhaps because arkdpi vaccines display increased heterogeneity, the results on cell immune responses have shown ambiguity. in the current study, we investigated the effects of vaccination with a highly stable and homogeneous massachusetts (mass)-type vaccine on day ... | 2020 | 32267130 |
| nationwide survey of infectious bronchitis virus on layer farms in japan during 2015. | after accumulating data through a nationwide survey, we characterized the recent prevalences and geographic distributions of various genotypes of infectious bronchitis virus (ibv) on layer farms in japan. reverse transcription pcr analysis of fecal samples revealed the presence of the ibv nucleoprotein (n) gene on approximately 30% of the farms surveyed. n-gene detection rates were higher in the chugoku and kyushu regions than in the remaining surveyed regions. phylogenetic analysis of s1 gene s ... | 2020 | 32267128 |
| infectious bronchitis virus population structure defines immune response and protection. | a commercial arkansas (ark) delmarva poultry industry (dpi)-type vaccine and a more homogeneous population of that vaccine obtained previously through adaptation to chicken embryo kidney (cek) cells (cek-arkdpi) were used as a model to further understand the impact of population genetic structure on generation of immune responses and protection. in a first experiment, vaccinated chickens were challenged with an ibv ark99-type virulent strain (al/4614/98). despite extensive sequence similarity be ... | 2020 | 32267126 |
| limited protection conferred by recombinant newcastle disease virus expressing infectious bronchitis spike protein. | recombinant newcastle disease virus (ndv) lasota (ls) expressing secreted trimeric spike (s)-ectodomain (se) of infectious bronchitis virus (ibv) (rls/ibv.se) was developed and evaluated for protection conferred against ibv challenge. the ibv s-ectodomain protein, which is s excluding the transmembrane anchor and short cytoplasmic domain of s2, expressed from recombinant ls corresponds to an arkansas (ark)-type ibv. in a first experiment, chickens were primed at 1 day of age or primed at 1 day o ... | 2020 | 32267125 |
| cytokine responses in tracheas from major histocompatibility complex congenic chicken lines with distinct susceptibilities to infectious bronchitis virus. | the chicken major histocompatibility complex (mhc) b locus has been linked to resistance to infectious diseases. we have previously provided evidence that the mhc congenic chicken lines 331/b2 and 335/b19 differ in susceptibility to infectious bronchitis virus (ibv) strains m41 and arkdpi in in vivo challenge experiments. innate immune responses can be difficult to measure in vivo because they are nonspecific and can be triggered by environmental factors. in an attempt to address this issue, we ... | 2020 | 32267123 |
| intestinal tropism of an infectious bronchitis virus isolate not explained by spike protein binding specificity. | an infectious bronchitis virus (ibv) with an unusual enteric tropism (calent) was isolated from a california broiler flock exhibiting runting-stunting syndrome. ibv was detected in the small intestine, but not in the respiratory tract or kidney. during virus isolation in embryos, it did not replicate in chorioallantoic membrane (cam) but could be recovered from intestines. its s1 protein showed 93% amino acid sequence identity to a california variant isolated in 1999 (cal99). intestinal lesions ... | 2020 | 32267122 |
| protection following simultaneous vaccination with three or four different attenuated live vaccine types against infectious bronchitis virus. | two or more different live attenuated infectious bronchitis virus (ibv) vaccine types are often given to broilers to induce homologous protection as well as to broaden protection against other ibv types in the field. however, the ability of broilers to respond to three or four different antigenic types of ibv vaccine has not been examined experimentally. in this study, we vaccinated one-day-old broiler chicks by eyedrop with three or four different ibv vaccine types simultaneously. the presence ... | 2020 | 32242456 |
| multiple recombination events between field and vaccine strains resulted in the emergence of a novel infectious bronchitis virus with decreased pathogenicity and altered replication capacity. | in this study, we isolated and identified 2 infectious bronchitis virus (ibv) strains from layer chickens soon after vaccination with the massachusetts-connecticut bivalent vaccine (conn) and h120 and 4/91 booster vaccines in china in 2011. the results of cross-virus-neutralization tests and phylogenetic analysis of the s1 subunit of spike gene of these vaccine strains and other reference strains showed that strain ljl/110302 was of gi-19 lineage, whereas lln/111169 was of the gi-1 lineage of th ... | 2020 | 32241473 |
| genetic characterization of infectious bronchitis viruses in thailand, 2014-2016: identification of a novel recombinant variant. | infectious bronchitis (ib) causes severe economic losses to the poultry industry worldwide owing to frequent emergence of novel infectious bronchitis virus (ibv) variants, which potentially affect the effectiveness of the currently used ibv vaccine. therefore, continuous monitoring of ibv genotypes and lineages recently circulating in chickens worldwide is essential. in this study, we characterized the complete s1 gene from 120 ibvs circulating in chickens in thailand from 2014 to 2016. phylogen ... | 2020 | 32241468 |
| genetic sequence changes related to the attenuation of avian infectious bronchitis virus strain tw2575/98. | the attenuated avian infectious bronchitis virus (ibv), derived from a wild strain (tw2575/98w) in chicken embryos after 75 passages, is designed as a commercial vaccine strain (tw2575/98vac) to control the disease in taiwan. the differences in viral infectivity, replication efficiency, and genome sequences between tw2575/98w and tw2575/98vac were determined and compared. tw2575/98vac caused earlier death of chicken embryos and had higher viral replication efficiency. thirty amino acid substitut ... | 2020 | 32232712 |
| rapid detection of avian infectious bronchitis virus by reverse transcriptase-loop mediated isothermal amplification. | a reverse-transcription loop mediated isothermal amplification (rt-lamp) was developed for rapid diagnosis of infectious bronchitis (ib) in poultry by targeting the spike protein 2 gene (s2). rt-lamp primers were designed for ibv-s2 targets and optimized to run at 60 °c for 45 min. as compared with rt-pcr, rt-lamp was 100 times more sensitive for ibv-s2 gene. rt-lamp showed specific amplification with ib viral genome but not with other avian respiratory pathogens due to their mismatching with ib ... | 2015 | 32226206 |
| detection of 793/b serotype of infectious bronchitis virus in tissue sample by indirect immunoperoxidase assay. | the indirect immunoperoxidase (iip) assay was compared with the reverse transcription-polymerase chain reaction (rt-pcr) for detection of 793/b serotype of infectious bronchitis virus in tissues samples collected from experimentally infected chickens. this technique was optimized in specific pathogen-free (spf)-embryonated chicken eggs and broiler chickens inoculated with the iranian ir/773/2001 strain of 793/b serotype the trachea, lung, kidney, and cecal tonsil tissue samples from experimental ... | 2014 | 32214974 |
| evaluation of sialic acid and acute-phase proteins (haptoglobin and serum amyloids a) in healthy and avian infection bronchitis virus-infected chicks. | forty-five 24-day-old cobb chicks infected with infectious bronchitis virus (ibv) and ten healthy 24-day-old cobb chicks without any clinical signs of ibv as control group were selected for the study. all of the diseased chicks showed some or all of the clinical signs of infectious bronchitis including gasping, coughing and nasal discharge, wet eyes, swollen sinuses, reduction of food consumption and weight gain. diagnosis of ibv was based on clinical signs and elisa test. blood samples were tak ... | 2011 | 32214968 |
| diverse gammacoronaviruses detected in wild birds from madagascar. | to date, infectious bronchitis virus (ibv) is potentially found in wild birds of different species. this work reports the survey of coronaviruses in wild birds from madagascar based on the targeting of a conserved genome sequence among different groups of covs. phylogenetic analyses revealed the presence of gammacoronaviruses in different species of gruiformes, passeriformes, ciconiiformes, anseriformes, and charadriiformes. furthermore, some sequences were related to various ibv strains. aquati ... | 2015 | 32214942 |
| genome sequencing and characterization analysis of a beijing isolate of chicken coronavirus infectious bronchitis virus. | avian infectious bronchitis virus (aibv) is classified as a member of the genus coronavirus in the family coronaviridae. the enveloped virus has a positive-sense, single-stranded rna genome of approximately 28 kilo-bases, which has a 5' cap structure and 3' polyadenylation tract. the complete genome sequence of infectious bronchitis virus (ibv), beijing isolate, was determined by cloning sequencing and primer walking. the whole genome is 27733 nucleotides in length, has ten open reading frames: ... | 2004 | 32214717 |
| optimization of transfection methods for huh-7 and vero cells: a comparative study. | availability of an efficient transfection protocol is the first determinant in success of gene transferring studies in mammalian cells which is accomplished experimentally for every single cell type. herein, we provide data of a comparative study on optimization of transfection condition by electroporation and chemical methods for huh-7 and vero cells. different cell confluencies, dna/reagent ratios and total transfection volumes were optimized for two chemical reagents including jetpei™ and lip ... | 2012 | 32214542 |
| n-glycosylation of infectious bronchitis virus m41 spike determines receptor specificity. | infection of chicken coronavirus infectious bronchitis virus (ibv) is initiated by binding of the viral heavily n-glycosylated attachment protein spike to the alpha-2,3-linked sialic acid receptor neu5ac. previously, we have shown that n-glycosylation of recombinantly expressed receptor binding domain (rbd) of the spike of ibv-m41 is of critical importance for binding to chicken trachea tissue. here we investigated the role of n-glycosylation of the rbd on receptor specificity and virus replicat ... | 2020 | 32213247 |
| pathogenicity and genome changes in qx-like infectious bronchitis virus during continuous passaging in embryonated chicken eggs. | infectious bronchitis (ib) remains a major problem in the global poultry industry despite the many available vaccines. live attenuated vaccines are the most effective means of preventing ib and are traditionally generated by serial passaging of a wild strain in embryonated chicken eggs. in this study, the sz isolate of the qx-like infectious bronchitis virus (ibv) was continuously passaged in chicken embryos for 250 passages. we compared the pathogenicity of different passages (sz50, sz100, sz15 ... | 2020 | 32142742 |
| real-time, minion-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples. | infectious bronchitis (ib) causes significant economic losses in the global poultry industry. control of ib is hindered by the genetic diversity of the causative agent, infectious bronchitis virus (ibv), which has led to the emergence of several serotypes that lack complete serologic cross-protection. although serotyping requires immunologic characterization, genotyping is an efficient means to identify ibvs detected in samples. sanger sequencing of the s1 subunit of the spike gene is currently ... | 2020 | 32133932 |
| a novel low virulent respiratory infectious bronchitis virus originating from the recombination of qx, tw and 4/91 genotype strains in china. | in china, variants of infectious bronchitis virus (ibv) evolve continually and diverse recombinant strains have been reported. here, an ibv strain, designated as ck/ch/ljx/2017/07 (referred as jx17) was isolated from chicken vaccinated with h120 and 4/91 in jiangxi, china, in 2017. sequence analysis reveals of the s1 gene of jx17 the highest nucleotide identity of 98.15% with that of gi-7 genotype tw2575/98 strain. furthermore, whole genome analysis among jx17 and other 18 ibv strains demonstrat ... | 2020 | 32122588 |
| complete genome sequence of avian coronavirus strain ga08 (gi-27 lineage). | avian coronavirus, also known as infectious bronchitis virus, is a highly contagious respiratory pathogen of chickens that is responsible for major economic losses to the poultry industry around the globe. here, we report the complete genome sequence of strain ga08 of the gi-27 lineage, isolated from a fecal sample from a broiler chicken collected in georgia in 2015. | 2020 | 32107296 |
| 16s rrna gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus. | infectious bronchitis virus (ibv), a member of the coronaviridae family, causes serious losses to the poultry industry. intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading pathogens. the aim of this study was to investigate the link between the intestinal microbiome and nephropathogenic ibv (nibv) infection. initially, chickens were randomly distributed into 2 groups: the normal group (inc) and the infected group (iibv). t ... | 2020 | 32103130 |
| molecular survey and interaction of common respiratory pathogens in chicken flocks (field perspective). | the present study was designed for the detection of the most prevalent respiratory infections in chicken flocks and clarifying their interaction and impact on flock health. | 2019 | 32095050 |
| expression and immunoreactivity of a recombinant multi-epitope antigen designed based on four major structural proteins of avian infectious bronchitis virus. | the development of rapid, simple, and sensitive diagnostic methods for identification of avian infectious bronchitis virus (ibv) is crucial for the effective control of avian infectious bronchitis. in the present study, a tandemly arranged multiepitope peptide (named semn) was designed with four antigenic regions derived from four major structural proteins of ibv. then, we performed codon optimization of semn gene by changing the codon-adaptation index from 0.45 to 0.94 and expressed the optimiz ... | 2020 | 32089981 |
| pathological and virological analysis of concurrent disease of chicken anemia virus infection and infectious bronchitis in japanese native chicks. | a concurrent infection of chicken anemia virus (cav) and infectious bronchitis virus (ibv) was detected in japanese native chicks in 2017, in which a high mortality rate (97.7%) was recorded in a small flock of 130 chicks exhibiting poor growth. histological examination revealed that the affected chicks exhibited two different pathological entities: one was severe hematopoietic and lymphocytic depletion with abnormally large cells containing intranuclear inclusion bodies of cav, whereas the othe ... | 2020 | 32074517 |
| effect of tlr agonist on infections bronchitis virus replication and cytokine expression in embryonated chicken eggs. | avian infectious bronchitis (ib) is an acute, highly infectious and contagious viral disease of chickens caused by avian infectious bronchitis virus (ibv) belonging to the genus coronavirus and family coronaviridae. it can affect all age groups of birds. the toll-like receptors (tlrs) are a major class of innate immune pattern recognition receptors that have a key role in immune response and defense against various infections.the tlrs are essential for initiation of innate immune responses and i ... | 2020 | 32065987 |
| recombinant infectious bronchitis coronavirus h120 with the spike protein s1 gene of the nephropathogenic ibyz strain remains attenuated but induces protective immunity. | infectious bronchitis (ib) is a highly infectious viral disease responsible for major economic losses in the poultry industry. a reverse genetic vaccine is a safe, rapid, and effective method of achieving ib prevention and control. in this study, we constructed the recombinant strain, rh120-s1/yz, using a reverse genetic system, based on the backbone of the h120 vaccine strain, with the s1 gene replaced with that of the qx-like nephropathogenic strain, ck/ch/ibyz/2011, isolated in china. the res ... | 2020 | 32057575 |
| change of pathotype and phylogenetic analysis of infectious bronchitis virus detected in kagoshima prefecture, japan. | infectious bronchitis (ib) is a highly contagious disease in chickens, induced by ib virus (ibv) infection. the pathotype and s1 genotype of ibv field strain that was detected from 2008 to 2018 were investigated in kagoshima prefecture, japan. the frequency of cases that the renal lesion characteristic of ibv infection was histopathologically confirmed was significantly higher from 2014 to 2018 than from 2008 to 2009, suggesting the altered pathotype of ibv. of 7 genotypes (jp-i, jp-ii, jp-iii, ... | 2020 | 32037366 |
| research note: first evidence of infectious bronchitis virus middle-east gi-23 lineage (var2-like) in germany. | the infectious bronchitis virus middle-east gi-23 lineage (var2-like) was observed on a german broiler farm, for the first time. the animals suffered from respiratory and nephropathogenic disease. gross lesions observed during necropsy included tracheitis, aerosacculitis, and nephritis. tracheal swabs were tested positive for infectious bronchitis virus middle-east gi-23 lineage (var2-like) by pcr. furthermore, sequence analysis of the s1 spike protein showed close relationship to the commercial ... | 2020 | 32036979 |
| mirna repertoire and host immune factor regulation upon avian coronavirus infection in eggs. | avian infectious bronchitis virus (ibv) is a coronavirus with great economic impact on the poultry industry, causing an acute and highly contagious disease in chickens that primarily affects the respiratory and reproductive systems. the cellular regulation of ibv pathogenesis and the host immune responses involved remain to be fully elucidated. micrornas (mirnas) have emerged as a class of crucial regulators of numerous cellular processes, including responses to viral infections. here, we employ ... | 2020 | 32025807 |
| isolation and characterization of avian coronavirus from healthy eclectus parrots (eclectus roratus) from indonesia. | avian coronavirus has a wide range of hosts, from chickens and turkeys to wild birds. this virus causes an economically and, possibly, environmentally, important loss in the poultry industry. therefore, research into the avian coronavirus in various species of birds is required. the eclectus parrot (eclectus roratus) is an endemic bird to indonesia and northern australia and often kept as pets. at present, there has been limited information about avian coronavirus infection among birds. this stu ... | 2019 | 32009759 |
| regulation of immunity-related genes by infectious bronchitis virus challenge in spleen of laying chickens. | understanding of host pathogen interactions is important in planning strategies for effective control of the pathogen. the present study investigated the regulation of genes involved in the activation of splenic immune system in mature laying chickens challenged with t strain of infectious bronchitis virus (ibv). among all the genes studied, the relative expression levels of fas cell surface death receptor (fas), interleukin 7 (il7), il18, proteasome subunit alpha 3 (psma3), major histocompatibi ... | 2020 | 31985363 |
| a recombinant infectious bronchitis virus from a chicken with a spike gene closely related to that of a turkey coronavirus. | using viral metagenomics, the complete genome sequence of an infectious bronchitis virus (ibv) strain (named ahysx-1) from a fecal sample from a healthy chicken in anhui province, china, was determined. the genome sequence of ahysx-1 was found to be very similar to that of ibv strain ck/ch/lln/131040 (kx252787), except for the spike gene region, which is similar to that of a turkey coronavirus strain (eu022526), suggesting that ahysx-1 is a recombinant. recombination analysis and phylogenetic an ... | 2020 | 31982944 |
| gga-mir-30d regulates infectious bronchitis virus infection by targeting usp47 in hd11 cells. | avian infectious bronchitis virus (ibv) is a coronavirus which infects chickens and causes severe economic losses to the poultry industry worldwide. micrornas (mirnas) are important intracellular regulators and play a pivotal role in viral infections. in previous studies, we have revealed that ibv infection caused a significant down-regulation of gga-mir-30d expression in chicken kidneys. in present study, we investigated the role of gga-mir-30d in the process of ibv infection of hd11 cell line ... | 2020 | 31982568 |
| characterization of an outbreak of infectious coryza (avibacterium paragallinarum) in commercial chickens in central california. | in 2017, the turlock branch of the california animal health & food safety laboratory system received a significant increase in infectious coryza (ic) necropsy cases, with a total of 54 submissions originating from commercial broilers (n = 40), commercial layers (n = 11), and backyard chickens (n = 3). layer flocks positive for ic were distributed within the adjacent counties of merced and stanislaus, while broiler flocks were concentrated within merced county. the backyard flocks were located in ... | 2019 | 31967433 |
| qx-like infectious bronchitis virus reported in india. | 2020 | 31953290 | |
| characterization of infectious bronchitis virus d181, a new serotype (gii-2). | this paper describes the characterization of a new infectious bronchitis virus (ibv) strain d181, that rapidly evolved from a low-level incidental finding in 2017 to become the second most isolated ibv strain in dutch layers and breeders in 2018, as well as being found in samples from germany and belgium. based on the sequence of the s gene and the results of cross-neutralization tests, d181 can be considered as a new serotype and the second lineage within genotype ii (gii-2). the experimental i ... | 2020 | 31951468 |
| molecular characterization of variant infectious bronchitis virus in china, 2019: implications for control programmes. | infectious bronchitis virus (ibv), an ongoing emergence enveloped virus with a single-stranded positive-sense rna genome, belongs to the gammacoronavirus genus in the coronaviridae family. ibv-associated tracheitis, nephritis, salpingitis, proventriculitis and egg drop have caused devastating economic losses to poultry industry worldwide. since the end of 2018, a remarkably increasing number of commercial broilers and layers, vaccinated or not, were infected with ibv in china. here, we described ... | 2020 | 31943814 |
| author correction: a recombinant newcastle disease virus (ndv) expressing s protein of infectious bronchitis virus (ibv) protects chickens against ibv and ndv. | an amendment to this paper has been published and can be accessed via a link at the top of the paper. | 2020 | 31937880 |
| molecular epidemiology of infectious bronchitis virus in poland from 1980 to 2017. | the presence of infectious bronchitis virus (ibv) was identified for the first time in the poultry population in poland at the end of the 1960s. from this time a few waves of epidemics caused by different ibv variants spread across the country. in order to gain more insight into the molecular epidemiology of ibv in poland, in the present study the s1 coding region of 34 ibv isolates and nearly whole genome of 10 strains collected over a period of 38 years was characterized. phylogenetic analysis ... | 2020 | 31917362 |
| genetic, antigenic and pathogenic characterization of avian coronaviruses isolated from pheasants (phasianus colchicus) in china. | two viruses were isolated in 2017 from commercial pheasants with severe clinical signs and mortality in shandong and anhui provinces, china, respectively. we examined the pathogenic effects of the viruses in chicken embryos and the size and morphology of the virus particles, performed phylogenetic analysis based on the s1 gene and complete genomic sequences, and examined the antibody responses against infectious bronchitis virus (ibv). the results suggested that the viruses i0623/17 and i0710/17 ... | 2020 | 31902509 |
| antiviral responses against chicken respiratory infections: focus on avian influenza virus and infectious bronchitis virus. | some of the respiratory viral infections in chickens pose a significant threat to the poultry industry and public health. in response to viral infections, host innate responses provide the first line of defense against viruses, which often act even before the establishment of the infection. host cells sense the presence of viral components through germinal encoded pattern recognition receptors (prrs). the engagement of prrs with pathogen-associated molecular patterns leads to the induction of pr ... | 2020 | 31901597 |
| effect of ibv d1466 on egg production and egg quality and the effect of heterologous priming to increase the efficacy of an inactivated ibv vaccine. | to protect layers, breeders and grandparents against damage by infectious bronchitis virus infections during the laying period, vaccination using live priming followed by a boost with inactivated ib vaccine is commonly used. for many ib variants, homologous live vaccines are not available for priming. very little is known about the efficacy of priming with heterologous live ib vaccines (or combination of live ib vaccines) to induce broad ib protection in long-living chickens. in this study, the ... | 2020 | 31899942 |
| a simple and rapid diagnostic method to detect new goose astrovirus using reverse-transcription loop-mediated isothermal amplification. | in this study, we develop a reverse-transcription loop-mediated isothermal amplification (rt-lamp) assay for rapid and easy detection of goose astroviruses (gastvs) in clinical samples. the specific lamp primer sets were designed targeting the orf2 gene of gastv. the conditions of lamp amplification were optimized in terms of reaction time and temperature. the optimal conditions are 60 min in a 60 °c water bath. no cross-reactivity was noted with fowl adenovirus serotype 4 (fadv-4), duck tembusu ... | 2020 | 31879584 |
| antigenic and pathogenic characteristics of qx-type avian infectious bronchitis virus strains isolated in southwestern china. | the qx-type avian infectious bronchitis virus (ibv) is still a prevalent genotype in southwestern china. to analyze the antigenicity and pathogenicity characteristics of the dominant genotype strains (qx-type), s1 gene sequence analysis, virus cross-neutralization tests, and pathogenicity test of eight qx-type ibv isolates were conducted. sequence analysis showed that the nucleotide homology between the eight strains was high, but distantly related to h120 and 4/91 vaccine strains. cross-neutral ... | 2019 | 31847269 |
| evaluation of bacteriophage efficacy in reducing the impact of single and mixed infections with escherichia coli and infectious bronchitis in chickens. | infectious bronchitis virus (ibv) represents a major threat to poultry production worldwide particularly when complicated with bacterial infection. in the present study samples were collected from forty broiler farms with respiratory manifestations to characterize ibv and e. coli. bacteriophages were isolated and enriched from sampled farms to study its efficacy to control single and mixed infections with e. coli and ibv in vivo. twelve out of forty farms were positive for ibv. phylogenetic anal ... | 2019 | 31839902 |
| attenuated live infectious bronchitis virus qx vaccine disseminates slowly to target organs distant from the site of inoculation. | infectious bronchitis (ib) is a highly contagious respiratory disease of poultry, caused by the avian coronavirus infectious bronchitis virus (ibv). currently, one of the most relevant genotypes circulating worldwide is ibv-qx (gi-19), for which vaccines have been developed by passaging virulent qx strains in embryonated chicken eggs. here we explored the attenuated phenotype of a commercially available qx live vaccine, ib primo qx, in specific pathogens free broilers. at hatch, birds were inocu ... | 2020 | 31822427 |
| attenuation and amino acid changes in infectious bronchitis virus iranian 793/b serotype during serial passaging in embryonated chicken eggs. | the use of live attenuated vaccine (lav) is the main method for controlling infectious bronchitis (ib). it is advisable to develop a lav using a dominant serotype in the region in the case of vaccine failure. since 793/b serotype is one of the most predominant circulating ib viruses in iran, attenuation of three iranian 793/b isolates (ir/773/2001, ir/794/2002 and ir/520/2002) was done by serial passaging in specific pathogen free (spf) embryonated chicken eggs up to 90 passages to assess the de ... | 2019 | 31802683 |
| retraction note to: mitochondrial bioenergy alterations in avian hd11 macrophages infected with infectious bronchitis virus. | the editor-in-chief has retracted this article [1]. figures 1a, 1d and 2b (bottom right) are identical with figures 1a, 1h and 1b respectively in another article [2] which reports a study in a different species. in addition, table 1 contains data presented in a third article [3], which also reports a study in a different species. the editor-in-chief therefore no longer has confidence in the validity of the data and the conclusions drawn. tereza c. cardoso disagrees with this retraction. helena l ... | 2020 | 31784908 |