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challenge infection model for mers-cov based on naturally infected camels.middle east respiratory syndrome coronavirus (mers-cov) is an emerging virus that infects humans and camels with no approved antiviral therapy or vaccine. some vaccines are in development for camels as a one-health intervention where vaccinating camels is proposed to reduce human viral exposure. this intervention will require an understanding of the prior exposure of camels to the virus and appropriate vaccine efficacy studies in camels.202032552831
some pathological observations on the naturally infected dromedary camels (camelus dromedarius) with the middle east respiratory syndrome coronavirus (mers-cov) in saudi arabia 2018-2019.background: the natural mers-cov infection in dromedary camels is understudied. recent experimental studies showed no obvious clinical signs in the infected dromedary camels.aim: to study the pathological changes associated with natural mers-cov infection in dromedary camels.methods: tissues from three mers-cov positive animals as well as two negative animals were collected and examined for the presence of pathological changes. the screening of the animals was carried out first by the rapid aggl ...202032543343
genetic diversity and population structure of dromedary camel-types.the dromedary camel is a unique livestock for its adaptations to arid-hot environments and its ability to provide goods under extreme conditions. there are no registries or breed standards for camels. thus, named camel populations (i.e., camel-types) were examined for genetic uniqueness and breed status. camel populations are generally named based on shared phenotype, country or region of origin, tribal ownership, or the ecology of their habitat. a dataset of 10 short-tandem repeat markers genot ...202032530038
alterations of hemogram, serum biochemistry, oxidative/nitrosative balance, and copper/zinc homeostasis in dromedary camels naturally infected with poxvirus.camel pox (cmlp), a contagious viral disease of camels, causes considerable economic loss in terms of milk, meat, wool, and leather production besides reduction of draught power. the effect of spontaneous cmlp infection on hemogram, oxidative/nitrosative imbalance, and trace mineral homeostasis has not been studied earlier in dromedary camels. in the current study, hemogram, serum biochemistry, oxidant/antioxidant imbalance, and zinc (zn)-copper (cu) homeostasis were evaluated in healthy and pox ...202032519073
detection of blood pathogens in camels and their associated ectoparasitic camel biting keds, hippobosca camelina: the potential application of keds in xenodiagnosis of camel haemopathogens.background: major constraints to camel production include pests and diseases. in northern kenya, little information is available about blood-borne pathogens circulating in one-humped camels ( camelus dromedarius) or their possible transmission by the camel haematophagous ectoparasite, hippobosca camelina, commonly known as camel ked or camel fly. this study aimed to: (i) identify the presence of potentially insect-vectored pathogens in camels and camel keds, and (ii) assess the potential utility ...201932510036
diagnosis and treatment of foreign bodies swallowing syndrome in camels (camelus dromedarius) with special reference to the role of mineral deficiency.this study describes the clinical presentation of ruminal and reticular foreign body syndrome (rrfbs), and evaluates the effect of mineral deficiency on its occurrence in dromedary camels. thirty dromedary camels were divided into two groups. group 1 (control) included 10 apparently healthy she-camels. group 2 consisted of twenty dromedary camels diagnosed with rrfbs on the basis of clinical, ultrasonographic, hematological, and biochemical examinations. clinical findings showed decreased appeti ...202032507784
successful vitrification of dromedary camel embryos using a novel embryo vitrification kit.embryos (n = 87) collected 8 days after mating and 7 days after ovulation were vitrified using a camel-specific vitrification kit. vitrification solutions (vs) containing 20% foetal calf serum, with or without 2% bovine serum albumin (bsa) were used to cryopreserve embryos, in three steps vs1 (5 min), vs2 (5 min) and vs3 (30-35 s) at room temperature (rt) before being loaded into open pulled straws and immediately frozen in liquid nitrogen. embryos were subsequently thawed in warming solutions ( ...202032507263
genomic sequencing and analysis of eight camel-derived middle east respiratory syndrome coronavirus (mers-cov) isolates in saudi arabia.middle east respiratory syndrome coronavirus (mers-cov) causes severe respiratory illness in humans; the second-largest and most deadly outbreak to date occurred in saudi arabia. the dromedary camel is considered a possible host of the virus and also to act as a reservoir, transmitting the virus to humans. here, we studied evolutionary relationships for 31 complete genomes of betacoronaviruses, including eight newly sequenced mers-cov genomes isolated from dromedary camels in saudi arabia. throu ...202032503352
gangrenous mastitis in dromedary camels in uae caused by streptococcus agalactiae.mastitis is a disease of economic concern that affects dairy industry worldwide. this study aimed to investigate and identify possible etiologies encountered in an episode of acute gangrenous mastitis in lactating she-camels in al dhafra region, abu dhabi emirate, united arab emirates (uae). beside the routine clinical examination, conventional bacteriological methods were used to isolate and identify possible aerobic/anaerobic bacterial or fungal pathogens from cultured milk samples collected f ...202032493341
genome-wide diversity, population structure and demographic history of dromedaries in the central desert of iran.the development of camel husbandry for good production in a desert climate is very important, thus we need to understand the genetic basis of camels and give attention to genomic analysis. we assessed genome-wide diversity, linkage disequilibrium (ld), effective population size (ne) and relatedness in 96 dromedaries originating from five different regions of the central desert of iran using genotyping-by-sequencing (gbs). a total of 14,522 single nucleotide polymorphisms (snps) with an average m ...202032485848
cross-sectional prevalence study of mers-cov in local and imported dromedary camels in saudi arabia, 2016-2018.the middle east respiratory syndrome-coronavirus (mers-cov) is an endemic virus in dromedaries. annually, saudi arabia imports thousands of camels from the horn of africa, yet the epidemiology of mers-cov in these animals is largely unknown. here, mers-cov prevalence was compared in imported african camels and their local counterparts. a total of 1399 paired sera and nasal swabs were collected from camels between 2016 and 2018. imported animals from sudan (n = 829) and djibouti (n = 328) were sa ...202032453746
exploring the eukaryotic diversity in rumen of indian camel (camelus dromedarius) using 18s rrna amplicon sequencing.in addition to a wide variety of anaerobic and facultative anaerobic bacteria, camel rumen also harbors a diverse of eukaryotic organisms. in the present study, the eukaryotic communities of camel rumen were characterized using 18s rrna amplicon sequencing. metagenomic dna was isolated from rumen samples of fourteen adult bikaneri and kachchhi breeds of camel fed different diets containing jowar, bajra, maize, and guar. illumina sequencing generated 27,161,904 number of reads corresponding to 15 ...202032448959
seroprevalence of dromedary camel hev in domestic and imported camels from saudi arabia.hepatitis e virus (hev) imposes a major health concern in areas with very poor sanitation in africa and asia. the pathogen is transmitted mainly through ingesting contaminated water or food, coming into contact with affected people, and blood transfusions. very few reports including old reports are available on the prevalence of hev in saudi arabia in humans and no reports exist on hev prevalence in camels. dromedary camel trade and farming are increasing in saudi arabia with importation occurri ...202032443401
genital abnormalities associated to lack of uterine adenogenesis or endometrial gland dysgenesis of female dromedary camels (camelus dromedarius).the developmental disruption of the müllerian duct and the endometrial dynamic can generate genital lesions that could contribute to infertility.202032426256
snp discovery and population structure analysis in lassi and marecha camel breeds using a genotyping by sequencing method.pakistani camels have been classified socio-geographically into 20 breeds, but they have not yet been subjected to substantial selective pressures and the genetic basis for these breeds is not understood. however, it should be possible to distinguish them by use of molecular data. this study investigated the genetic diversity and population structure within and between two major pakistani camel breeds, marecha and lassi. as no snp array is currently available, we first identified 63 619 snps usi ...202032421863
preservation of the spermatozoa of the dromedary camel (camelus dromedarius) by chilling and freezing: the effects of cooling time, extender composition and catalase supplementation.this study sought to determine the characteristics of dromedary camel sperm following 24 h chilling and cryopreservation, testing two different buffers and cryoprotectants and the presence of catalase (500 iu/ml). ejaculates were liquefied in tris-citric acid-fructose buffer, and centrifuged through a colloid. for experiment 1 (n = 5) sperm were cooled 24 h in green buffer or inra-96® containing 0 or 3% glycerol or ethylene glycol. experiment 2 (n = 5) used the same six treatments to evaluate sp ...202032416545
intrafollicular spontaneous parthenogenetic development of dromedary camel oocytes.dromedary camel oocytes are unique in their capability for intrafollicular and in vitro spontaneous parthenogenetic activation (spa) and development. this study was designed for (a) observing the incidence of spa and development of dromedary camel oocytes retrieved from ovaries; (b) assessing intrafollicular development of dromedary camel oocytes using histological examination; (c) evaluating the abilities of dromedary camel oocytes to mature, spa, and develop in vitro; and (d) identifying the t ...202032415813
scanning electron microscopy of onchocerca fasciata (filarioidea: onchocercidae) adults, microfilariae and eggs with notes on histopathological findings in camels.onchocerca fasciata is a prevalent filarial species in camelids of asia and africa forming nodules in the skin of dromedary and bactrian camels. in spite of recent advances in the biology and epidemiology of this nematode species, a relatively scant number of studies have focussed on the morphology of this parasite. the main objective of this study was to describe morphological characteristics of adults, microfilariae and eggs of o. fasciata by scanning electron microscopy (sem), staining and hi ...202032404208
a pilot study on the pharmacokinetics of a single intramuscular injection of cefquinome in arabian camel calves.this study was conducted to evaluate the pharmacokinetics of cefquinome in camel calves after a single intramuscular injection in a dose of 2 mg/kg body weight (kg b. w.). cefquinome concentrations were measured by ultra-high performance liquid chromatography-mass spectrometry (uplc-ms/ms). a non-compartmental pharmacokinetic model was used to fit the time-concentration curve and estimate the pharmacokinetic parameters. the peak serum concentration (cmax) was 28.4 μg/ml at the time of maximum co ...202032384074
morphometric characterization and estimating body weight of two algerian camel breeds using morphometric measurements.this study was carried out in order to identify the body measurements of two different dromedary camel breeds raised in algeria. the animal material of the study consisted of a total of 115 animals belong to steppe (n = 55) and sahraoui (n = 60) camel breeds. eye and coat colors along with body measurements such as head length, neck length, neck girth, tail length, distance between eyes, distance between ears, body length, withers height, chest girth, and live weight were determined. least squar ...202032377968
the dipeptidyl peptidase-4 expression in some mers-cov naturally infected dromedary camels in saudi arabia 2018-2019.mers-cov usually causes respiratory and renal failure in some patients, which may be the underlying cause of death. dromedary camels are the only known reservoir of the virus until now. they shed the virus in their body secretions thus potentiate a risk for human infection. mers-cov tropism and replication is mainly affected by the presence of certain receptor ligands on the target tissues. the dipeptidyl peptidase-4 (dpp-4) is believed to act as receptors for mers-cov. the main objective of thi ...202032377556
comparative analysis of milk fat globular membrane (mfgm) proteome between saudi arabia camelus dromedary safra and wadha breeds.camel milk is traditionally known to have medicinal properties and many potential health benefits. natural milk contains many soluble proteins and nanoparticles, such as a milk fat globule membrane (mfgm), a three-layered membrane covering of milk fat globule mainly composed of proteins and lipids, which plays an important role in human health. mfgm proteins account for 1%-4% of total milk proteins, and their nutritive value and distribution depends on the different breeds. the differential comp ...202032375319
detection of the middle east respiratory syndrome coronavirus in dromedary camel's seminal plasma in saudi arabia 2015-2017.the middle east respiratory syndrome coronavirus (mers-cov) is an emergent respiratory virus. dromedary camels are currently the only known reservoir of mers-cov and are capable of transmitting the virus within a herd. the role of semen in the transmission of mers-cov has never been investigated as yet, to the best of our knowledge. our goal was to test semen collected from dromedary camels for mers-cov. a total of 67 seminal plasma samples from infertile and 13 from fertile dromedary camels wer ...202032374945
bacterial disease of dromedaries and bacterian camels. 198732370336
trypanosomiasis in the camel (camelus dromedarius). 198732370333
molecular evolution and structural mapping of n-terminal domain in spike gene of middle east respiratory syndrome coronavirus (mers-cov).the middle east respiratory syndrome coronavirus (mers-cov) is a lethal zoonotic pathogen circulating in the arabian peninsula since 2012. there is no vaccine for mers and anti-viral treatment is generally not applicable. we investigated the evolution of the mers-cov spike gene sequences and changes in viral loads over time from patients in saudi arabia from 2105-2017. all the mers-cov strains belonged to lineage 5, and showed high sequence homology (99.9%) to 2017 strains. recombination analysi ...202032370153
genetic diversity and population genetic structure of six dromedary camel (camelus dromedarius) populations in saudi arabia.camels are an integral and essential component of the saudi arabian heritage. the genetic diversity and population genetic structure of dromedary camels are poorly documented in saudi arabia so this study was carried out to investigate the genetic diversity of both local and exotic camel breeds. the genetic diversity was evaluated within and among camel populations using 21 microsatellite loci. hair and blood samples were collected from 296 unrelated animals representing 4 different local breeds ...202032346350
preliminary survey for antibodies against respiratory viruses among slaughter camels (camelus dromedarius) in north-eastern nigeria. 198932344952
effects of short-term inhibition of rho kinase on dromedary camel oocyte in vitro maturation.this is the first report on a biphasic in vitro maturation (ivm) approach with a meiotic inhibitor to improve dromedary camel ivm. spontaneous meiotic resumption poses a major setback for in vitro matured oocytes. the overall objective of this study was to improve in vitro maturation of dromedary camel oocytes using rock inhibitor (y-27632) in a biphasic ivm to prevent spontaneous meiotic resumption. in the first experiment, we cultured immature cumulus-oocyte complexes (cocs, n = 375) in a prem ...202032344840
properties of coronavirus and sars-cov-2.were identified beginning with the discovery of sars-cov in 2002. with the recent detection of sars-cov-2, there are now seven human coronaviruses. those that cause mild diseases are the 229e, oc43, nl63 and hku1, and the pathogenic species are sars-cov, mers-cov and sars-cov-2 coronaviruses (order nidovirales, family coronaviridae, and subfamily orthocoronavirinae) are spherical (125nm diameter), and enveloped with club-shaped spikes on the surface giving the appearance of a solar corona. withi ...202032342926
virulence, antimicrobial resistance and phylogenetic analysis of zoonotic walking pneumonia mycoplasma arginini in the one-humped camel (camelus dromedarius).in the scientific literature, a small amount of information is found concerning mycoplasmosis in camel species. a variety of pathogens could be causative agents for pneumonia, but walking pneumonia is mostly caused by mycoplasma with slow development and mild symptoms. the aim of this study was to identify mycoplasmas from camels (camelus dromedarius) and extending the arsenal of factors implicated in pathogenicity of m. arginini to shed light on the current knowledge gap. 460 lung samples (pneu ...202032330451
microbial quality and formation of biogenic amines in the meat and edible offal of camelus dromedaries with a protection trial using gingerol and nisin.this study aimed firstly at the investigation of the microbial status of the camel meat and edible offal including liver, kidneys, lungs, rumen, and duodenum distributed at local markets of egypt. total plate count, total psychrophilic counts, total enterobacteriaceae count, the most probable number of coliforms, and total mold counts were monitored at the collected samples. the produced biogenic amines (ba) in the camel meat and offal were further estimated. an experimental trial to investigate ...202032328276
superovulation of dromedary camels with ecg: an effective and practical technique.this study was conducted to develop simple superovulation protocols for dromedary camels using ecg. in experiment 1, camels received either 1000, 2000, 3000, 4000, 5000 or 6000 iu ecg. in experiment 2, camels received either 400 mg fsh (folltropin-v) twice-daily over 5 days or 3000 iu ecg. in experiment 3, camels received 3000 iu ecg either at 2, 3, 4 or 5 days after ovulation induction. ovarian response and embryo yield were evaluated in all experiments and embryos collected from camels treated ...202032325323
first serological evidence of the rift valley fever phlebovirus in tunisian camels.rift valley fever (rvf) is a mosquito-borne zoonosis that severely impacts livelihoods, national and international economies, and human health. few studies have investigated the prevalence of this infection in tunisian livestock. the present report aimed to update the epidemiological status and identify the risk factors associated with this rvf virus infection in the one-humped dromedary camel from arid areas. a total of 470 sera of apparently healthy camels (camelus dromedarius) were collected ...202032325049
investigation of a cluster of severe respiratory disease referred from uganda to kenya, february 2017.on february 22, 2017, hospital x-kampala and us cdc-kenya reported to the uganda ministry of health a respiratory illness in a 46-year-old expatriate of company a. the patient, mr. a, was evacuated from uganda to kenya and died. he had recently been exposed to dromedary camels (mers-cov) and wild birds with influenza a (h5n6). we investigated the cause of illness, transmission, and recommended control. we defined a suspected case of severe acute respiratory illness (sari) as acute onset of fever ...202032324075
calving date and its variability as a potential trait in the breeding objective to account for reproductive seasonality in alpacas.the low fertility and offspring survival indicators in alpacas can be partially due to their particularity seasonal reproduction that reduces the opportunities of the females to become pregnant within a season, with the survival of the offspring concerned by the availability of food and exposure to diseases that depends on the calving date. optimizing the date of delivery and reducing its variability are shown as eligible criteria that could be used as selection criteria within the genetic impro ...202032320085
ca2+ ions promote fusion of middle east respiratory syndrome coronavirus with host cells and increase infectivity.fusion with, and subsequent entry into, the host cell is one of the critical steps in the life cycle of enveloped viruses. for middle east respiratory syndrome coronavirus (mers-cov), the spike (s) protein is the main determinant of viral entry. proteolytic cleavage of the s protein exposes its fusion peptide (fp), which initiates the process of membrane fusion. previous studies on the related severe acute respiratory syndrome coronavirus (sars-cov) fp have shown that calcium ions (ca2+) play an ...202032295925
nucleocapsid gene analysis from an imported case of middle east respiratory syndrome coronavirus, malaysia.to describe the complete nucleocapsid (n) gene region of middle east respiratory syndrome coronavirus (mers-cov) from imported case in malaysia and the relations with human- and camel-derived mers-cov.201532289031
emerging developments on pathogenicity, molecular virulence, epidemiology and clinical symptoms of current middle east respiratory syndrome coronavirus (mers-cov).middle east respiratory syndrome coronavirus (mers-cov) is a recently reported virus that is associated with severe, life threatening and rapidly spreading primarily respiratory illness called the middle east respiratory syndrome. mers-cov possesses a unique positive-sense single-stranded rna and can undergo rapid mutation in the viral genome. this results in antigenic switching and genetic variation, finally leading to the emergence of novel and new mers-cov subtypes which are uncontrollable by ...201732288937
foot and mouth disease virus serological study of dromedary camels in oman.the potential role of camels in the epidemiology of foot and mouth disease in oman was investigated. sera from local dromedaries (n = 151) that graze with animals (cattle and small ruminants) positive for foot and mouth disease virus (fmdv) non-structural protein antibody (nsp-ab) were tested for the detection of fmdv nsp-ab. the samples were tested using a commercial competitive enzyme-linked immunosorbent assay (celisa) , a rapid immunochromatographic assay and a solid-phase celisa for the det ...201932286571
piebald camels.animal breeds are the diverse outcome of the thousands-year-long process of livestock domestication. many of these breeds are piebald, resulting from the artificial selection by pastoralists of animals bearing a genetic condition known as leucism, and selected for their productive, behavioural, or aesthetical traits. piebald dromedary camels have not been studied or discussed before, and their same existence is often overlooked. based on fieldwork in western sahara, direct observations across no ...201732269746
middle east respiratory syndrome coronavirus (mers-cov) seropositive camel handlers in kenya.middle east respiratory syndrome (mers) is a respiratory disease caused by a zoonotic coronavirus (mers-cov). camel handlers, including slaughterhouse workers and herders, are at risk of acquiring mers-cov infections. however, there is limited evidence of infections among camel handlers in africa. the purpose of this study was to determine the presence of antibodies to mers-cov in high-risk groups in kenya. sera collected from 93 camel handlers, 58 slaughterhouse workers and 35 camel herders, we ...202032260186
field investigation and phylogenetic characterization of orf virus (orfv) circulating in small ruminants and pseudocowpoxvirus (pcpv) in dromedary camels of eastern sudan.in this study, livestock herders in eastern sudan were interviewed through structured questionnaire involved 14046 animals in 151 herds (87 camel herds, 51 sheep and 13 goats) from june to september of 2016 in showak area of gadarif state to get some epidemiological information on contagious ecthyma (ce) infection. 102 suspected cases of ce were investigated (38 sheep, 22 goats and 42 camels) by a second questionnaire focusing on age and sex of affected animals beside number and localization of ...202032258461
seroprevalence of toxoplasma gondii and neospora caninum in dromedary camels ( camelus dromedarius ) from saudi arabia.serological screening of 199 serum samples from dromedary camels-from different cities in saudi arabia-was performed using enzyme-linked immunosorbent assay for detecting antibodies against two cyst-forming coccidian parasites, namely toxoplasma gondii and neospora caninum. antibodies against t. gondii were detected in 68 (34.2%) samples, while those against n. caninum were present in 33 (16.6%) samples. the highest seroprevalence of t. gondii antibodies was reported in samples from taif (51.2%) ...202032236334
camel production systems in ethiopia: a review of literature with notes on mers-cov risk factors.camels are the most adapted species to the harsh conditions of arid/semi-arid rangelands of ethiopia where pastoralism is the dominant mode of life and mobility is an inherent strategy to efficiently utilize the spatially and temporally distributed pasture and water resources. usually, large numbers of camels and other domestic animals from many different herds/flocks congregate at watering sites, and this may create a perfect condition for disease transmission and spread among animals. the same ...201832226597
comparison of serological and molecular tests for detection of trypanosoma evansi in domestic animals from ghardaïa district, south algeria.trypanosoma evansi (t. evansi) is a hemoflagellate parasite that affects a broad range of mammalian hosts and that causes a disease called surra. diagnosis of surra based on clinical symptoms alone is inaccurate. therefore, a variety of serological and molecular diagnostic tests are used to assist in the detection of t. evansi infections. the aim of this study was to compare the diagnostic performance of four serological tests (catt/t.evansi, immune trypanolysis, elisa with purified variant surf ...202032222595
diagnostic evaluation of subclinical endometritis in dromedary camels.the objective of the present study was to evaluate the efficacy of various diagnostic methods and to estimate the prevalence of bacterial pathogens associated with subclinical endometritis (sce) in dromedary camels. during two consecutive breeding seasons, a total of 2122 infertile female dromedaries were assigned to this study and suspected cases of sce were identified using the established criteria which included failure to conceive after three or more consecutive matings with a fertile male, ...202032216929
detection of pancoronavirus using pcr in camelus dromedarius in iran (first report).coronaviride is a colossal family of viruses that cause a variety of diseases in humans and other animals. as of late, a novel coronavirus, not anterior-optically discerned in humans, has been identified in a denizen of the middle east. there is growing evidence that the camelus dromedarius is host species for the virus and plays an important role of a source of human infection. along these lines, the authors decided to detect coronaviruses in dromedary camels in two high-risk areas of iran by e ...201732214976
coxiella burnetii in camels (camelus dromedarius) from algeria: seroprevalence, molecular characterization, and ticks (acari: ixodidae) vectors.q fever is a widespread zoonotic disease caused by coxiella burnetii that most commonly infects not only a variety of mammals but also arthropods and in particularly ticks. the aim of this study was to detect c. burnetii infection in camels including ixodid ticks using serological and molecular assays. between july 2018 to june 2019, blood samples from 184 male and female camels (camelus dromedarius) were collected from 3 regions of south-east algeria and serum samples were tested for antibodies ...202032173315
subclinical mastitis in pastoralist dairy camel herds in isiolo, kenya: prevalence, risk factors, and antimicrobial susceptibility.mastitis is an important constraint to milk production in pastoralist camel (camelus dromedarius) herds in kenya. the objective of this study was to investigate the prevalence, risk factors, and bacterial panorama of subclinical mastitis (scm) in pastoralist camel herds in isiolo county, kenya. furthermore, antimicrobial susceptibility in udder pathogens was studied. a cross-sectional sample of 206 camels from 20 milking herds was screened using the california mastitis test (cmt), and quarter mi ...202032171518
zoonotic implications of camel diseases in iran.approximately 60% of all human pathogens and 75% of emerging infectious diseases are zoonotic (of animal origin). camel zoonotic diseases can be encountered in all camel-rearing countries. in this article, all studies carried out on camel zoonotic diseases in iran are reviewed to show the importance of camels for public health in this country. more than 900 published documents were systematically searched to find relevant studies from 1,890 until late 2018. the collected articles were classified ...202032160657
prevalence, distribution on host's body, and chemical control of camel ticks hyalomma dromedarii in the united arab emirates.camel farming remains a part of the culture of the arabian peninsula although modern methods have greatly increased camel densities in the entire region. in the united arab emirates (uae), camel production is threatened by tick parasitism. however, no study has considered assessing the magnitude of the problem in the uae. we conducted a study evaluating tick richness, abundance, and spatial distribution of ticks on camels in farms near al ain, uae. in addition, we conducted a survey of farm owne ...202032158160
analysis of variation in growth and spline-based growth models for marecha and lassi dromedary camels.camel is an important domestic animal that is well adapted to extremely harsh environments. due to its multi-purpose role, the camel is gaining importance, particularly as a meat producer. body weights at different ages and growth curves have been extensively studied in several livestock species. however, the information on the camel is very limited. we investigate patterns of variation in birth weight and weaning weight of two prominent breeds of camels in pakistan, namely marecha and lassi. a ...202032157518
genetic parameters of birth weight trait in dromedary camels (camelus dromedarius).birth weight data of dromedary calves from the database of one of the world's largest dairy herds (dubai, uae) were analyzed for the period from 2007 to 2018. the assessment included the data of 4124 camel calves that were classified into six ecotypes (emirate, emirate crossed, black, pakistanian, saudi-sudanian, and saudi crossed). the aim of the study was to describe the heritability of birth weight of calves and the breeding value of sires. genetic parameters of birth weight were estimated by ...202032157517
seroprevalence of middle east respiratory syndrome corona virus in dromedaries and their traders in upper egypt.camel trade in egypt depends mainly on importation. seemingly healthy imported camels are responsible for the ingress of serious diseases into egypt. a striking example of this concerning public health globally is the middle east respiratory coronavirus (mers-cov) which causes case fatalities of over 34%. here, we determined the seroepidemiological situation of the mers-cov in imported camels and their traders in upper egypt.202032146454
genotyping and antimicrobial susceptibility of clostridium perfringens isolated from dromedary camels, pastures and herders.the present study aimed to isolate and genotype c. perfringens from healthy and diarrheic dromedary camels, pastures and herders; and to evaluate and compare antimicrobial susceptibility of the isolates. a total of 262 (56.3%) c. perfringens isolates were recovered from 465 samples of healthy and diarrheic dromedary camels, pastures and herders. c. perfringens type a (75.2%), type b (4.2%), type c (13.7%) and type d (6.9%) were detected. c. perfringens type a with only cpa+ gene was found in 191 ...202032145560
molecular characterization and diagnostic investigations of rabies encephalitis in camels (camelus dromedaries) in oman: a retrospective study.the accurate and early diagnosis of rabies is critical for undertaking public health measures in animals. the aim of the current study was to identify the molecular characterization of the circulating rabies virus (rabv) among camels (camelus dromedaries) in oman and to evaluate the efficacy of the histopathology and reverse transcription polymerase chain reaction (rt-pcr) as diagnostic tools of acute rabies encephalitis in camels in comparison with direct fluorescent antibody test (dfat). of th ...202032124182
spatial association between primary middle east respiratory syndrome coronavirus infection and exposure to dromedary camels in saudi arabia.middle east respiratory syndrome coronavirus (mers-cov) is an emerging zoonotic disease. exposure to dromedary camels (camelus dromedaries) has been consistently considered the main source of primary human infection. although saudi arabia reports the highest rate of human mers-cov infection and has one of the largest populations of dromedary camels worldwide, their spatial association has not yet been investigated. thus, this study aimed to examine the correlation between the spatial distributio ...202032112508
ticks and associated pathogens in camels (camelus dromedarius) from riyadh province, saudi arabia.camel production in saudi arabia is severely affected by various diseases and by inadequate veterinary services. ticks and tick-borne pathogens (tbps) affect the health and wellbeing of camels consequently diminishing their productivity and performances. in addition, camels may act as hosts for tbps (e.g. anaplasma phagocytophilum) causing diseases in humans. the current study aimed to determine the prevalence of ixodid ticks and molecularly investigate the associated pathogens in camels from sa ...202032111251
ultrasonography of the liver in healthy and diseased camels (camelus dromedaries).in camels, hepatic diseases are relatively common and most of them are misdiagnosed as a cause of illness because signs may be subtle. in addition, diagnostic laboratory methods are insufficient as hepatic enzymes can also be elevated in camels with cardiac or skeletal muscle damage. examples of liver diseases in camels are hepatic lipidosis, hepatitis, cirrhosis, hepatic necrosis, choleostasis, hyperplasia of biliary epithelium, hydatid cysts, glycogen deposition, cholangitis, cholangiohepatiti ...202032101826
crimean-congo hemorrhagic fever virus endemicity in united arab emirates, 2019.we conducted a cross-sectional survey of crimean-congo hemorrhagic fever virus (cchfv) in dromedary camels and attached ticks at 3 locations in the united arab emirates. results revealed a high prevalence of cchfv-reactive antibodies in camels and viral rna in ticks and camel serum, suggesting the virus is endemic in this country.202032097111
evidence of peste des petits ruminants' virus in dromedary camels in the kingdom of saudi arabia between 2014 and 2016.infection with the peste des petits ruminants virus (pprv) is a highly devastating viral infection of small ruminants. dromedary camels live in close proximity of small ruminants in arabian peninsula (ap) and many other regions in the world. little is known about the reasons behind continuous pprv emergence in saudi arabia (ksa). our objective was to test some dromedary camel population across the kingdom for the presence of specific pprv antibodies. our results show detection of specific pprv a ...201932089813
preliminary comparative assessment of brucellergene skin test for diagnosis of brucellosis in dromedary camels (camelus dromedarius).this study was conducted to evaluate the use of brucellergene skin test (bst) for the diagnosis of brucellosis in camels (camelus dromedarius) in comparison with rose bengal test (rbt) and competitive enzyme-linked immunosorbent assay (c-elisa). a total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of abu dhabi emirate, united arab emirates (uae), were included in the study. the skin test was applied on two shaved areas at the middle ...202032077807
isolation and characterization of vaginal lactobacillus spp. in dromedary camels (camelus dromedarius): in vitro evaluation of probiotic potential of selected isolates.lactobacillus spp. is one of the beneficial lactic acid producing microbiota in the vagina, which is important for a healthy vaginal environment. however, little is known about vaginal lactobacillus in dromedary camels (camelus dromedarius). therefore, this study aimed to isolate vaginal lactic acid bacteria (lab) in dromedary camels and to study the probiotic potential of selected isolates. a total of 75 vaginal swabs were collected from pluriparous, non-pregnant, non-lactating dromedary camels ...202032071813
dromedary camel cd14high mhciihigh monocytes display inflammatory properties and are reduced in newborn camel calves.in human and different animal species, blood monocytes are classified based on their expression pattern of different monocytic markers into phenotypically and functionally different subsets. in the current study, we used flow cytometry and monoclonal antibodies to cd172a, cd14, cd163 and mhcii to identify monocyte subsets in peripheral blood of dromedary camels.202032070351
meat quality characteristics of the arabian camel (camelus dromedarius) at different ages and post-mortem ageing periods.meat quality characteristics and sensory attributes were evaluated in three age groups (12, 18, and 24 mo) of one-humped camels of the saudi arabian najdi breed.202032054218
determination of zinc in camel skin using laser-induced breakdown spectroscopy.zinc plays a major role in skin integrity, which can be affected by dromedary camels' hard life conditions. deficiencies in some trace elements especially in zinc can explain susceptibility of this species to skin diseases. compared with ruminants, camel is already known for his relatively low zincemia. in order to assess dromedary camels' skin zinc content, the present study was carried out in several provinces located in the south of morocco where camel skin diseases are commonly observed. zin ...202032034682
ovary and ovarian bursa in dromedary camels: clinical relevance of the topographical features.this study aimed to associate ovarian characteristics with the efficiency of clinical examination and occurrence of genital diseases in dromedary camels. the reproductive tract of 870 female camels was examined through standard transrectal palpation and by ultrasonography during the breeding season. the ovaries were examined for structures and dimensions. the follicles were categorized according to size, the thickness of the wall and contents. follicle aspiration was carried out from females wit ...202032030811
camelid type i interferons: identification and functional characterization of interferon alpha from the dromedary camel (camelus dromedarius).investigations into the molecular immune response of dromedary camel, a key livestock species of the arid, have been limited due to the lack of species-specific reagents. here we describe for the first time, the identification and characterization of type i ifns of dromedary camel, which are the most important cytokines in the innate host immune response against viruses. we cloned camel ifn-α coding sequences and identified a total of eleven subtypes. the canonical ifn-α subtype designated as if ...202032014632
nanobody against pdl1.programmed death ligand 1 (pdl1, cd274, b7-h1) has been identified as the ligand for the immune inhibitory receptor programmed death 1 protein (pd1/pdcd1). pdl1 is a member of b7 family of immune molecules and this protein together with pdl2, are two ligands for pd1 expressed on activated lymphoid cells. by binding to pd1 on activated t cells, pdl1 may inhibit t cell responses by inducing apoptosis. accordingly, it leads to the immune evasion of cancers and contribute to tumor growth, thus pdl1 ...202032006351
seroprevalence of mers-cov in healthy adults in western saudi arabia, 2011-2016.the middle east respiratory syndrome coronavirus (mers-cov) is a newly recognized zoonotic coronavirus. current evidence confirms the role of dromedaries in primary human infections but does not explain the sporadic community cases. however, asymptomatic or subclinical cases could represent a possible source of infection in the community.202032005618
salmonella enterica and theileria co-infection in dromedary camels (camelus dromedarius) in uae.despite a steady increase in camel husbandry worldwide, pathology of camel diseases is still relatively under-investigated. clinical hematuria is generally indicative of either acute or chronic urogenital inflammations, traumatic calculous injuries, cancers, corrosive poisonings. infectious agents are not typically implicated in urinary tract infection of camels.201931998621
comparative evaluation of sedative and clinical effects of dexmedetomidine and xylazine in dromedary calves (camelus dromedarius).to compare the sedative and clinical effects of intravenous (iv) administration of dexmedetomidine and xylazine in dromedary calves.202031992523
role of a noncanonical disulfide bond in the stability, affinity, and flexibility of a vhh specific for the listeria virulence factor inlb.a distinguishing feature of camel (camelus dromedarius) vhh domains are noncanonical disulfide bonds between cdr1 and cdr3. the disulfide bond may provide an evolutionary advantage, as one of the cysteines in the bond is germline encoded. it has been hypothesized that this additional disulfide bond may play a role in binding affinity by reducing the entropic penalty associated with immobilization of a long cdr3 loop upon antigen binding. to examine the role of a noncanonical disulfide bond on an ...202031981247
thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress.the arabian camel is the largest known mammal that can survive in severe hot climatic conditions. we provide the molecular explanation for the thermotolerance of camel granulosa somatic cells after exposure to 45 °c for 2 (acute heat shock) or 20 h (chronic heat shock). the common features of the cellular responses to acute heat stress were the increase of heat shock proteins and dna repair enzymes expression. actin polymerization and rho signaling were critically activated as a cellular defense ...202031969994
middle east respiratory syndrome coronavirus antibodies in bactrian and hybrid camels from dubai.so far, dromedary camels are the only known animal reservoir for middle east respiratory syndrome (mers) coronavirus (mers-cov). previous published serological studies showed that sera of bactrian camels were all negative for mers-cov antibodies. however, a recent study revealed that direct inoculation of bactrian camels intranasally with mers-cov can lead to infection with abundant virus shedding and seroconversion. in this study, we examined the presence of mers-cov antibodies in bactrian and ...202031969478
comprehensive genomic analysis of the dromedary t cell receptor gamma (trg) locus and identification of a functional trgc5 cassette.the emergent availability in public databases of more complete genome assemblies allows us to improve genomic data obtained by classical molecular cloning. the main goal of this study was to refine the genomic map of the dromedary trg locus by integrating our previous genomic data with the analysis of recent genomic assemblies. we identified an additional trgc cassette, defined as a v-j-c recombination unit, located at the 5' of the locus and made up of five trgv genes followed by three trgj gen ...202031962062
middle east respiratory syndrome coronavirus transmission.middle east respiratory syndrome coronavirus (mers-cov) infection causes a spectrum of respiratory illness, from asymptomatic to mild to fatal. mers-cov is transmitted sporadically from dromedary camels to humans and occasionally through human-to-human contact. current epidemiologic evidence supports a major role in transmission for direct contact with live camels or humans with symptomatic mers, but little evidence suggests the possibility of transmission from camel products or asymptomatic mer ...202031961300
prevalence of trypanosoma evansi in livestock in palestine.trypanosoma evansi is the causative agent of surra, a disease that occurs in many animal species. the disease is responsible for substantial losses in global production and can be fatal if not diagnosed early. this study aims to determine the prevalence of t. evansi in livestock, equids and dromedary camels in palestine.202031931864
whole-genome sequencing of 128 camels across asia reveals origin and migration of domestic bactrian camels.the domestic bactrian camels were treated as one of the principal means of locomotion between the eastern and western cultures in history. however, whether they originated from east asia or central asia remains elusive. to address this question, we perform whole-genome sequencing of 128 camels across asia. the extant wild and domestic bactrian camels show remarkable genetic divergence, as they were split from dromedaries. the wild bactrian camels also contribute little to the ancestry of domesti ...202031925316
anatomical features in the kidney involved in water conservation through urine concentration in dromedaries (camelus dromedarius).the aim of this study was to report some of the morphological characteristics of the kidney involved in urine concentration and hence water conservation in the dromedaries. a total of 20 fresh kidneys of 10 apparently healthy camels were used in this study. the architecture of the renal pelvis was revealed by dissection and polyvinyl chloride corrosion casts. samples were also processed for histology and for enzyme histochemistry. the camel kidney is bean shaped, smooth, multilobar, unipapillary ...202031922050
comparison between the effects of adding vitamins, trace elements, and nanoparticles to shotor extender on the cryopreservation of dromedary camel epididymal spermatozoa.there are several obstacles in camel semen cryopreservation; such as increasing semen viscosity and the reduction in motile spermatozoa after ejaculation. epididymal spermatozoa offer an efficient alternative to overcome these problems and are well-suited for artificial insemination in camels. in the current study, we compared the effects of supplementation with vitamin c, e, inorganic trace elements of selenium (na2seo3) and zinc (znso4), and zinc and selenium nanoparticles (znonps and senps, r ...202031906462
camelids: new players in the international animal production context.the camelidae family comprises the bactrian camel (camelus bactrianus), the dromedary camel (camelus dromedarius), and four species of south american camelids: llama (lama glama), alpaca (lama pacos) guanaco (lama guanicoe), and vicuña (vicugna vicugna). the main characteristic of these species is their ability to cope with either hard climatic conditions like those found in arid regions (bactrian and dromedary camels) or high-altitude landscapes like those found in south america (south american ...202031898022
antigen capture enzyme-linked immunosorbent assay for detecting middle east respiratory syndrome coronavirus in humans.the middle east respiratory syndrome (mers) is the second novel zoonotic disease infecting humans caused by coronavirus (cov) in this century. to date, more than 2200 laboratory-confirmed human cases have been identified in 27 countries, and more than 800 mers-cov associated deaths have been reported since its outbreak in 2012. rapid laboratory diagnosis of mers-cov is the key to successful containment and prevention of the spread of infection. though the gold standard for diagnosing mers-cov in ...202031883089
zoonotic parasites of dromedary camels: so important, so ignored.with a global population of about 35 million in 47 countries, dromedary camels play a crucial role in the economy of many marginal, desert areas of the world where they survive under harsh conditions. nonetheless, there is scarce knowledge regarding camels' parasite fauna which can reduce their milk and meat productions. in addition, only scattered information is available about zoonotic parasites transmitted to humans via contamination (e.g. cryptosporidium spp., giardia duodenalis, balantidium ...201931881926
considerations for the development of a dromedary camel (camelus dromedarius) semen collection centre.the dromedary camel (dc) is a strategic animal for the exploitation of the desert and unhospitable lands (arid and semiarid areas). these animals are a genetic resource, locally adapted and more resilient to these environs, that may significantly contribute to food security and sustainable development of marginal land areas. artificial insemination is the least invasive, least expensive technique for improving genetic selection and minimising transmission of venereal disease among animals and he ...202031864495
parasitological, serological and molecular survey of camel trypanosomiasis in somalia.camel trypanosomiasis or surra is of great concern in somalia, since the country possesses the largest one-humped camel (camelus dromedarius) population in the world. civil war in somalia has resulted in the destruction of educational, research, economic and social structures, making the country scores very low for most humanitarian indicators. previous studies on detection of trypanosoma species in somali camels have only been performed during the 1990s using standard trypanosome detection meth ...201931864389
influenza a virus infections in dromedary camels, nigeria and ethiopia, 2015-2017.we examined nasal swabs and serum samples acquired from dromedary camels in nigeria and ethiopia during 2015-2017 for evidence of influenza virus infection. we detected antibodies against influenza a(h1n1) and a(h3n2) viruses and isolated an influenza a(h1n1)pdm09-like virus from a camel in nigeria. influenza surveillance in dromedary camels is needed.202031855544
seroprevalence and molecular characterization of foot-and-mouth disease virus in chad.this study aimed at determining the seroprevalence of foot-and-mouth disease (fmd) in domestic ruminants and at characterizing the virus strains circulating in four areas of chad (east batha, west batha, wadi fira and west ennedi). the study was carried out between october and november 2016. a total of 1,520 sera samples (928 cattle, 216 goats, 254 sheep and 122 dromedaries) were collected randomly for fmd serological analyses. nine epithelial tissue samples were also collected from cattle showi ...202031845545
indigenous medicinal uses, toxicity, and chemical composition of browsing plant used by camel in ethiopia somali regional state: a survey.camels (camelus dromedarius) have access to different browsing plant that is a major source of feed and therapeutic value but also has the potential to cause poisoning to the camel. a survey was conducted between july and september 2017 to generate information on medicinal uses, toxicity, and chemical composition of major browsing plant in the somali regional state of ethiopia. a total of 150 respondents were interviewed using a structured questionnaire, and supplementary information was gathere ...202031845116
enzootic patterns of middle east respiratory syndrome coronavirus in imported african and local arabian dromedary camels: a prospective genomic study.the middle east respiratory syndrome coronavirus (mers-cov) is a lethal zoonotic pathogen endemic to the arabian peninsula. dromedary camels are a likely source of infection and the virus probably originated in africa. we studied the genetic diversity, geographical structure, infection prevalence, and age-associated prevalence among camels at the largest entry port of camels from africa into the arabian peninsula.201931843456
preservation of dromedary camel embryos at 4 °c for up to 5 days: factors affecting the pregnancy and pregnancy loss rates.the aims of the present study were to evaluate the effect of cooling of the dromedary camel embryos on the pregnancy and pregnancy loss rates, and to investigate the factors which might affect the outcomes of the transfer of cooled embryos. after the donors (n = 56) had been super-ovulated and mated, they were flushed at day 8 or 9 post-mating. of 487 collected embryos, 110 were refrigerated at 4°c for up to 5 days in holding medium (hm), flushing medium supplemented with 10% fetal calf serum (f ...202031835099
camelids and cattle are dead-end hosts for peste-des-petits-ruminants virus.peste-des-petits-ruminants virus (pprv) causes a severe respiratory disease in small ruminants. the possible impact of different atypical host species in the spread and planed worldwide eradication of pprv remains to be clarified. recent transmission trials with the virulent pprv lineage iv (liv)-strain kurdistan/2011 revealed that pigs and wild boar are possible sources of pprv-infection. we therefore investigated the role of cattle, llamas, alpacas, and dromedary camels in transmission trials ...201931817946
histological characterization of the lingual tonsils of the one-humped camel (camelus dromedarius).tonsils are located in the entrance of digestive and respiratory tracts forming waldeyer's ring that reacts against ingested or inhaled antigens. on occasion, tonsils may be a site of entry and replication for some pathogens. the lingual tonsils are a main constituent of the waldeyer's ring. despite the immunological importance of the lingual tonsils, there is limited information about their structure in the one-humped camel. the lingual tonsils of 10 clinically healthy male camels (3-25 years) ...202031811405
middle east respiratory syndrome coronavirus (mers-cov) neutralising antibodies in a high-risk human population, morocco, november 2017 to january 2018.backgroundmiddle east respiratory syndrome coronavirus (mers-cov) remains a major concern for global public health. dromedaries are the source of human zoonotic infection. mers-cov is enzootic among dromedaries on the arabian peninsula, the middle east and in africa. over 70% of infected dromedaries are found in africa. however, all known zoonotic cases of mers have occurred in the arabian peninsula with none being reported in africa.aimwe aimed to investigate serological evidence of mers-cov in ...201931796154
a review of asymptomatic and subclinical middle east respiratory syndrome coronavirus infections.the epidemiology of middle east respiratory syndrome coronavirus (mers-cov) since 2012 has been largely characterized by recurrent zoonotic spillover from dromedary camels followed by limited human-to-human transmission, predominantly in health-care settings. the full extent of infection of mers-cov is not clear, nor is the extent and/or role of asymptomatic infections in transmission. we conducted a review of molecular and serological investigations through pubmed and embase from september 2012 ...201931781765
molecular basis of binding between middle east respiratory syndrome coronavirus and cd26 from seven bat species.continued reports of middle east respiratory syndrome coronavirus (mers-cov) infecting humans have occurred since the identification of this virus in 2012. mers-cov is prone to cause endemic disease in the middle east, with several dozen spillover infections to other continents. it is hypothesized that mers-cov originated from bat coronaviruses and that dromedary camels are its natural reservoir. although gene segments identical to mers-cov were sequenced from certain species of bats and one spe ...202031776269
relationship between concentrations of macro and trace elements in serum and follicular, oviductal, and uterine fluids of the dromedary camel (camelus dromedarius).this study aimed at investigating the relationship between concentrations of macro and trace elements in blood serum, and fluids from small and large follicles (sff and lff, respectively), oviduct (of), and uterus (uf) of female dromedary camels. fluids from small (2-6 mm) and large follicles (7-20 mm), oviduct and uterus, and blood samples were collected from 19 camels. the results indicated that the concentrations of serum mg, fe, and mn were significantly higher than their follicular fluid, o ...202031760562
performance of an immunochromatographic test (ict) in comparison to some commonly used serological tests for the diagnosis of brucellosis in dromedary camels (camelus dromedarius).serological tests may represent an essential tool for the diagnosis of camel brucellosis; however, concerns arise in the scientific community regarding the direct transposition from cattle and small ruminants without adequate validation. the present study was made to compare four serological tests for the diagnosis of brucellosis in dromedary camels (camelus dromedarius). in terms of sensitivity, our results show that the immunochromatographic test (ict) shows the higher value of sensitivity, 98 ...201931757071
efficiency of commercial egg yolk-free and egg yolk-supplemented tris-based extenders for dromedary camel semen cryopreservation.this study compared the efficiency of commercial egg yolk-free (andromed, optixcell) and egg yolk-supplemented (triladyl, steridyl) tris-based extenders for semen cryopreservation in seven adult dromedary camels. the camel-specific extender shotor was used as control. the collected semen samples were evaluated and diluted with shotor, triladyl, steridyl, andromed, or optixcell. the diluted semen was gradually cooled and equilibrated for two hours before liquid nitrogen freezing. semen was evalua ...201931752406
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