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the challenge of monitoring elusive large carnivores: an accurate and cost-effective tool to identify and sex pumas (puma concolor) from footprints.acquiring reliable data on large felid populations is crucial for effective conservation and management. however, large felids, typically solitary, elusive and nocturnal, are difficult to survey. tagging and following individuals with vhf or gps technology is the standard approach, but costs are high and these methodologies can compromise animal welfare. such limitations can restrict the use of these techniques at population or landscape levels. in this paper we describe a robust technique to id ...201728273159
functional responses of cougars (puma concolor) in a multiple prey-species system.the study of predator-prey interactions is commonly analyzed using functional responses to gain an understanding of predation patterns and the impact they have on prey populations. despite this, little is known about predator-prey systems with multiple prey species in sites near the equator. here we studied the functional response of cougars (puma concolor) in sierra nanchititla natural reserve (mexico), in relation to their main prey, armadillo (dasypus novemcinctus), coati (nasua narica) and w ...201728261959
evolutionary and functional mitogenomics associated with the genetic restoration of the florida panther.florida panthers are endangered pumas that currently persist in reduced patches of habitat in south florida, usa. we performed mitogenome reference-based assemblies for most parental lines of the admixed florida panthers that resulted from the introduction of female texas pumas into south florida in 1995. with the addition of 2 puma mitogenomes, we characterized 174 single nucleotide polymorphisms (snps) across 12 individuals. we defined 5 haplotypes (pco1-pco5), one of which (pco1) had a geogra ...201728204600
assessing timing and causes of neonatal lamb losses in a bighorn sheep ovis canadensis canadensis herd via use of vaginal implant transmitters.we evaluated the use of vaginal implant transmitters (vits) as a means of detecting, capturing, and radio collaring rocky mountain bighorn sheep ( ovis canadensis canadensis) lambs to estimate survival and to facilitate carcass recovery to assess causes of mortality. we focused on one of several bighorn herds in colorado, usa, suffering from depressed recruitment that was not preceded by a classic all-age die-off. we captured, radio-collared, diagnosed pregnancy by ultrasound examination, and in ...201728192042
attitudes toward jaguars and pumas and the acceptability of killing big cats in the brazilian atlantic forest: an application of the potential for conflict index2.we explored the overall acceptability of killing jaguars and pumas in different scenarios of people-big cat interactions, the influence of attitudes toward big cats on acceptability, and the level of consensus on the responses. data were obtained from 326 self-administered questionnaires in areas adjacent to intervales state park and alto ribeira state park. overall, people held slightly positive attitudes toward jaguars and pumas and viewed the killing of big cats as unacceptable. however, indi ...201728188582
incorporating periodic variability in hidden markov models for animal movement.clustering time-series data into discrete groups can improve prediction and provide insight into the nature of underlying, unobservable states of the system. however, temporal variation in probabilities of group occupancy, or the rates at which individuals move between groups, can obscure such signals. we use finite mixture and hidden markov models (hmms), two standard clustering techniques, to model long-term hourly movement data from florida panthers (puma concolor coryi). allowing for tempora ...201728149522
jaguar interactions with pumas and prey at the northern edge of jaguars' range.we present the first study that evaluates jaguar-puma interactions in the arid lands of northern mexico, where jaguars have their northernmost breeding population and both predators are persecuted for livestock depredation. we tested whether jaguars are the dominant species in this unique ecosystem, where: (1) pumas outnumber jaguars, (2) pumas are better adapted to arid environments, and (3) jaguars and pumas are of similar size. we analyzed four years of data with two approaches; a two species ...201728133569
photoreceptor degeneration in a mountain lion cub (puma concolor).an orphaned 4-mo-old female mountain lion cub ( puma concolor ) was captured along the coastline in montaña de oro state park in los osos, california, usa. following suspicion that the cub was visually impaired, ophthalmic examination revealed diffuse bilateral retinal atrophy. due to a poor prognosis, humane euthanasia was elected. necropsy and histopathological findings were consistent with photoreceptor degeneration. based on the cub's signalment, history, and histopathology, a genetic or nut ...201628080904
dental and temporomandibular joint pathology of the california mountain lion (puma concolor couguar).skulls from 91 california mountain lions (puma concolor couguar) were examined macroscopically and radiographically. the majority of the skulls were from young adult animals (57.1%). the skull specimens were from 42 male (46.1%) and 34 female (37.4%) animals, while the gender was unknown for the remainder. the majority (94.5%) of teeth were present for examination. only 11 teeth were identified as absent congenitally; five of these teeth were maxillary first molar teeth and three were maxillary ...201728024874
human expansion precipitates niche expansion for an opportunistic apex predator (puma concolor).there is growing recognition that developed landscapes are important systems in which to promote ecological complexity and conservation. yet, little is known about processes regulating these novel ecosystems, or behaviours employed by species adapting to them. we evaluated the isotopic niche of an apex carnivore, the cougar (puma concolor), over broad spatiotemporal scales and in a region characterized by rapid landscape change. we detected a shift in resource use, from near complete specializat ...201628008961
feline immunodeficiency virus cross-species transmission: implications for emergence of new lentiviral infections.owing to a complex history of host-parasite coevolution, lentiviruses exhibit a high degree of species specificity. given the well-documented viral archeology of human immunodeficiency virus (hiv) emergence following human exposures to simian immunodeficiency virus (siv), an understanding of processes that promote successful cross-species lentiviral transmissions is highly relevant. we previously reported natural cross-species transmission of a subtype of feline immunodeficiency virus, puma lent ...201728003486
big cat coalitions: a comparative analysis of regional brain volumes in felidae.broad-based species comparisons across mammalian orders suggest a number of factors that might influence the evolution of large brains. however, the relationship between these factors and total and regional brain size remains unclear. this study investigated the relationship between relative brain size and regional brain volumes and sociality in 13 felid species in hopes of revealing relationships that are not detected in more inclusive comparative studies. in addition, a more detailed analysis ...201627812324
hunting as a management tool? cougar-human conflict is positively related to trophy hunting.overexploitation and persecution of large carnivores resulting from conflict with humans comprise major causes of declines worldwide. although little is known about the interplay between these mortality types, hunting of predators remains a common management strategy aimed at reducing predator-human conflict. emerging theory and data, however, caution that such policy can alter the age structure of populations, triggering increased conflict in which conflict-prone juveniles are involved.201627729084
puma (puma concolor) epididymal sperm morphometry.the andean puma (puma concolor) has not been widely studied, particularly in reference to its semen characteristics. the aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the hemacolor kit. morphometric data were obtained from 581 spermatozoa using a casa-morph system, rendering 13 mo ...201727678466
the occurrence of trichinella species in the cougar puma concolor couguar from the state of colorado and other regions of north and south america.trichinella species are zoonotic nematodes that infect wild carnivores and omnivores throughout the world. we examined the prevalence and species of trichinella infections in cougars (puma concolor couguar) from colorado, united states. tongues from cougars were examined by pepsin-hcl artificial digestion to detect trichinella spp. larvae. the species or genotype of individual worms was identified by multiplex polymerase chain reaction (pcr). trichinella spp. larvae were detected in 17 of 39 cou ...201727582037
interactions between demography, genetics, and landscape connectivity increase extinction probability for a small population of large carnivores in a major metropolitan area.the extinction vortex is a theoretical model describing the process by which extinction risk is elevated in small, isolated populations owing to interactions between environmental, demographic, and genetic factors. however, empirical demonstrations of these interactions have been elusive. we modelled the dynamics of a small mountain lion population isolated by anthropogenic barriers in greater los angeles, california, to evaluate the influence of demographic, genetic, and landscape factors on ex ...201627581877
molecular and morphological evidence of taenia omissa in pumas (puma concolor) in the peruvian highlands.a total of 41 cestodes were collected during necropsy examination on 2 pumas (puma concolor) that were found in 2 communities in canchis province, cuzco region, peru, at 4500 meters above sea level (peruvian andes). the cestodes were evaluated morphologically and molecularly. a fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) was used as a genetic marker. all the cestodes were identified as taenia omissa. in the present report, we give a brief description by molecular and ...201627580394
bartonella rochalimae and b. vinsonii subsp. berkhoffii in wild carnivores from colorado, usa.spleen samples from 292 wild carnivores from colorado, us were screened for bartonella infection. bartonella dna was detected in coyotes ( canis latrans ) (28%), striped skunks ( mephitis mephitis ) (23%), red foxes ( vulpes vulpes ) (27%), and raccoons ( procyon lotor ) (8%) but not in black bears ( ursus americanus ), gray foxes ( urocyon cinereoargenteus ), and mountain lions ( puma concolor ). two bartonella species, b. vinsonii subsp. berkhoffii and b. rochalimae, were identified. all 10 in ...201627529290
exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus and parvovirus in the cerrado of central brazil.human population growth around protected areas increases the contact between wild and domestic animals, promoting disease transmission between them. this study investigates the exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus (cdv) and parvovirus in emas national park (enp) in the cerrado savanna of central brazil. serum samples were collected from 169 wild carnivores, including the maned wolf (chrysocyon brachyurus), crab-eating fox (cerdocyon thous), hoary f ...201627469023
isolation of bartonella henselae, bartonella koehlerae subsp. koehlerae, bartonella koehlerae subsp. bothieri and a new subspecies of b. koehlerae from free-ranging lions (panthera leo) from south africa, cheetahs (acinonyx jubatus) from namibia and captive cheetahs from california.bartonellae are blood- and vector-borne gram-negative bacteria, recognized as emerging pathogens. whole-blood samples were collected from 58 free-ranging lions (panthera leo) in south africa and 17 cheetahs (acinonyx jubatus) from namibia. blood samples were also collected from 11 cheetahs (more than once for some of them) at the san diego wildlife safari park. bacteria were isolated from the blood of three (5%) lions, one (6%) namibian cheetah and eight (73%) cheetahs from california. the lion ...201627453220
first records of canis dirus and smilodon fatalis from the late pleistocene tule springs local fauna, upper las vegas wash, nevada.late pleistocene groundwater discharge deposits (paleowetlands) in the upper las vegas wash north of las vegas, nevada, have yielded an abundant and diverse vertebrate fossil assemblage, the tule springs local fauna (tslf). the tslf is the largest open-site vertebrate fossil assemblage dating to the rancholabrean north american land mammal age in the southern great basin and mojave desert. over 600 discrete body fossil localities have been recorded from the wash, including an area that now encom ...201627366649
lagochilascaris minor (nematoda: ascarididae) from a wild cougar ( puma concolor ) in mexico.we document parasitation of a wild cougar ( puma concolor ) by the nematode lagochilascaris minor in hidalgo state, mexico. this finding contributes to our understanding of the epidemiology of this zoonotic agent in mexico.201627310170
evaluating the responses of a territorial solitary carnivore to potential mates and competitors.successful communication is critical to the fitness of individuals and maintenance of populations, but less is known regarding the social contexts and reactions to scent marking by other individuals in solitary carnivores, including pumas. we evaluated the responses of resident male pumas to visitation and scent marking by potential competitors (other male pumas) and potential mates (female pumas) by capturing and marking 46 pumas (puma concolor), and documenting scent marking behaviours using m ...201627251230
pathogen exposure varies widely among sympatric populations of wild and domestic felids across the united states.understanding how landscape, host, and pathogen traits contribute to disease exposure requires systematic evaluations of pathogens within and among host species and geographic regions. the relative importance of these attributes is critical for management of wildlife and mitigating domestic animal and human disease, particularly given rapid ecological changes, such as urbanization. we screened > 1000 samples from sympatric populations of puma (puma concolor), bobcat (lynx rufus), and domestic ca ...201627209780
oxygen isotope composition of north american bobcat (lynx rufus) and puma (puma concolor) bone phosphate: implications for provenance and climate reconstruction.feline carnivores are threatened by illegal wildlife trade. tracing the provenance of unknown felid tissues via stable isotope analysis could provide important information in wildlife crime investigations. the oxygen isotope composition of mammalian skeletal phosphate (δ(18)op) is widely applied to trace the origin of animal remains and to reconstruct migratory patterns in palaeontological, archaeological, ecological and wildlife forensic applications. teeth and bones of terrestrial mammals form ...201627002600
isolation of bartonella henselae and two new bartonella subspecies, bartonellakoehlerae subspecies boulouisii subsp. nov. and bartonella koehlerae subspecies bothieri subsp. nov. from free-ranging californian mountain lions and bobcats.domestic cats are the natural reservoir of bartonella henselae, b. clarridgeiae and b. koehlerae. to determine the role of wild felids in the epidemiology of bartonella infections, blood was collected from 14 free-ranging california mountain lions (puma concolor) and 19 bobcats (lynx rufus). bartonella spp. were isolated from four (29%) mountain lions and seven (37%) bobcats. these isolates were characterized using growth characteristics, biochemical reactions, molecular techniques, including pc ...201626981874
a comparative analysis of genetic diversity and structure in jaguars (panthera onca), pumas (puma concolor), and ocelots (leopardus pardalis) in fragmented landscapes of a critical mesoamerican linkage zone.with increasing anthropogenic impact and landscape change, terrestrial carnivore populations are becoming more fragmented. thus, it is crucial to genetically monitor wild carnivores and quantify changes in genetic diversity and gene flow in response to these threats. this study combined the use of scat detector dogs and molecular scatology to conduct the first genetic study on wild populations of multiple neotropical felids coexisting across a fragmented landscape in belize, central america. we ...201626974968
canine distemper virus in wild felids of costa rica.several highly infectious diseases can be transmitted through feces and cause elevated mortality among carnivore species. one such infectious agent, canine distemper virus (cdv; paramyxoviridae: morbillivirus), has been reported to affect wild carnivores, among them several felid species. we screened free-ranging and captive wild carnivores in costa rica for cdv. between 2006 and 2012, we collected 306 fecal samples from 70 jaguars (panther onca), 71 ocelots ( leopardus pardalis ), five jaguarun ...201626967127
species-specific responses of carnivores to human-induced landscape changes in central argentina.the role that mammalian carnivores play in ecosystems can be deeply altered by human-driven habitat disturbance. while most carnivore species are negatively affected, the impact of habitat changes is expected to depend on their ecological flexibility. we aimed to identify key factors affecting the habitat use by four sympatric carnivore species in landscapes of central argentina. camera trapping surveys were carried out at 49 sites from 2011 to 2013. each site was characterized by 12 habitat att ...201626950300
data from camera surveys identifying co-occurrence and occupancy linkages between fishers (pekania pennanti), rodent prey, mesocarnivores, and larger predators in mixed-conifer forests.these data provide additional information relevant to the frequency of fisher detections by camera traps, and single-season occupancy and local persistence of fishers in small patches of forest habitats detailed elsewhere, "landscape fuel reduction, forest fire, and biophysical linkages to local habitat use and local persistence of fishers (pekania pennanti) in sierra nevada mixed-conifer forests" [10]. the data provides insight on camera trap detections of 3 fisher predators (bobcat [lynx rufus ...201626937448
ticks and rickettsiae from wildlife in belize, central america.the agents of spotted fevers in latin america are rickettsia rickettsii, r. parkeri, rickettsia sp. strain atlantic rainforest, and r. massiliae. in continental central america, r. rickettsii remains the only known pathogenic tick-borne rickettsia. in the present study, ticks were collected from wild mammals in natural areas of belize. besides providing new data of ticks from belize, we investigated rickettsial infection in some of these ticks. our results provide ticks harboring rickettsial age ...201626831147
interspecific interactions between wild felids vary across scales and levels of urbanization.ongoing global landscape change resulting from urbanization is increasingly linked to changes in species distributions and community interactions. however, relatively little is known about how urbanization influences competitive interactions among mammalian carnivores, particularly related to wild felids. we evaluated interspecific interactions between medium- and large-sized carnivores across a gradient of urbanization and multiple scales. specifically, we investigated spatial and temporal inte ...201526811767
hypercarnivorous apex predator could provide ecosystem services by dispersing seeds.large "hypercarnivorous" felids are recognized for their role as apex predators and hence as key elements in food webs and ecosystem functioning through competition and depredation. here we show that cougars (puma concolor), one of the largest and the most widely ranging apex felid predators with a strictly carnivorous diet, could also be effective secondary long distance seed dispersers, potentially establishing direct and non-herbivore mediated interactions with plant species at the bottom of ...201626791932
using species distribution models to predict potential landscape restoration effects on puma conservation.a mosaic of intact native and human-modified vegetation use can provide important habitat for top predators such as the puma (puma concolor), avoiding negative effects on other species and ecological processes due to cascade trophic interactions. this study investigates the effects of restoration scenarios on the puma's habitat suitability in the most developed brazilian region (são paulo state). species distribution models incorporating restoration scenarios were developed using the species' oc ...201626735128
[population estimates and conservation of felids (carnivora: felidae) in northern quintana roo, mexico].wildlife density estimates provide an idea of the current state of populations, and in some cases, reflect the conservation status of ecosystems, essential aspects for effective management actions. in mexico, several regions have been identified as high priority areas for the conservation of species that have some level of risk, like the yucatan peninsula (yp), where the country has the largest population of jaguars. however, little is known about the current status of threatened and endangered ...201526666134
weather and prey predict mammals' visitation to water.throughout many arid lands of africa, australia and the united states, wildlife agencies provide water year-round for increasing game populations and enhancing biodiversity, despite concerns that water provisioning may favor species more dependent on water, increase predation, and reduce biodiversity. in part, understanding the effects of water provisioning requires identifying why and when animals visit water. employing this information, by matching water provisioning with use by target species ...201526560518
ocelot population status in protected brazilian atlantic forest.forest fragmentation and habitat loss are detrimental to top carnivores, such as jaguars (panthera onca) and pumas (puma concolor), but effects on mesocarnivores, such as ocelots (leopardus pardalis), are less clear. ocelots need native forests, but also might benefit from the local extirpation of larger cats such as pumas and jaguars through mesopredator release. we used a standardized camera trap protocol to assess ocelot populations in six protected areas of the atlantic forest in southeaster ...201526560347
the role of scent marking in mate selection by female pumas (puma concolor).mate selection influences individual fitness, is often based on complex cues and behaviours, and can be difficult to study in solitary species including carnivores. we used motion-triggered cameras at 29 community scrapes (i.e. scent marking locations used by multiple individuals) and home range data from 39 gps-collared pumas (puma concolor) to assess the relevance of communication behaviours for mate selection by female pumas in california. female pumas visited community scrapes irregularly an ...201526489008
a conservation-based approach to compensation for livestock depredation: the florida panther case study.calf (bos taurus) depredation by the federally endangered florida panther (puma concolor coryi) on ranches in southwest florida is an important issue because ranches represent mixed landscapes that provide habitat critical to panther recovery. the objectives of this study were to (1) quantify calf depredation by panthers on two ranches in southwest florida, and (2) develop a habitat suitability model to evaluate the quality of panther hunting habitat on ranchlands, assess whether the model could ...201526421433
improvements on gps location cluster analysis for the prediction of large carnivore feeding activities: ground-truth detection probability and inclusion of activity sensor measures.animal space use studies using gps collar technology are increasingly incorporating behavior based analysis of spatio-temporal data in order to expand inferences of resource use. gps location cluster analysis is one such technique applied to large carnivores to identify the timing and location of feeding events. for logistical and financial reasons, researchers often implement predictive models for identifying these events. we present two separate improvements for predictive models that future p ...201526398546
spatial overlap in a solitary carnivore: support for the land tenure, kinship or resource dispersion hypotheses?there are several alternative hypotheses about the effects of territoriality, kinship and prey availability on individual carnivore distributions within populations. the first is the land-tenure hypothesis, which predicts that carnivores regulate their density through territoriality and temporal avoidance. the second is the kinship hypothesis, which predicts related individuals will be clumped within populations, and the third is the resource dispersion hypothesis, which suggests that resource r ...201626395576
inconsistency of allometric scaling for dissociative anesthesia of wild felids.to evaluate allometric scaling for ketamine-xylazine (kx) anesthesia in wild felids using domestic cats for reference.201626376437
space-use, movement and dispersal of sub-adult cougars in a geographically isolated population.cougar (puma concolor) observations have increased in midwest north america, with breeding populations re-establishing in several regions east of their contemporary range. the cypress hills uplands, located in southwest saskatchewan and southeast alberta, was recently re-colonized by cougars and now supports the easternmost confirmed breeding population of cougars in canada. a number of factors contribute to this cougar range expansion, but it is dispersal that provides the mechanism for re-colo ...201526290786
identification of novel gammaherpesviruses in ocelots (leopardus pardalis) and bobcats (lynx rufus) in panama and colorado, usa.gammaherpesviruses (ghvs) have been identified in many species and are often associated with disease. recently, we characterized three novel felid ghvs in domestic cats (felis catus), bobcats (lynx rufus), and pumas (puma concolor). we investigated whether free-ranging ocelots (leopardus pardalis) and bobcats are infected with additional ghvs. we screened dna samples from ocelots on barro colorado island, panama, and bobcats in western colorado, us, by using a degenerate nested pcr that targets ...201526280877
on the available names for the northern and eastern south american subspecies of puma concolor (linnaeus, 1771) (mammalia: felidae) and their type localities. 201326213758
survival and mortality of pumas (puma concolor) in a fragmented, urbanizing landscape.wide-ranging large carnivores pose myriad challenges for conservation, especially in highly fragmented landscapes. over a 13-year period, we combined monitoring of radio collared pumas (puma concolor) with complementary multi-generational genetic analyses to inform puma conservation in southern california, usa. our goals were to generate survivorship estimates, determine causes of mortality, identify barriers to movement, and determine the genetic and demographic challenges to puma persistence a ...201526177290
livestock predation by puma (puma concolor) in the highlands of a southeastern brazilian atlantic forest.we evaluated local opinion about reducing livestock losses to puma (puma concolor) and the potential for conflict among livestock breeders inside a protected area in the highlands of a southeastern brazilian atlantic forest. we also quantified the number and type of livestock losses, and determined if predation by puma was correlated with property profile and landscape characteristics. we conducted semistructured interviews with 42 livestock breeders sampled in 36 rural properties. when asked ho ...201526092051
the comparative effects of large carnivores on the acquisition of carrion by scavengers.pumas (puma concolor) and black bears (ursus americanus) are large carnivores that may influence scavenger population dynamics. we used motion-triggered video cameras deployed at deer carcasses to determine how pumas and black bears affected three aspects of carrion acquisition by scavengers: presence, total feeding time, and mean feeding-bout duration. we found that pumas were unable to limit acquisition of carrion by large carnivores but did limit aspects of carrion acquisition by both birds a ...201525996866
is there a single best estimator? selection of home range estimators using area-under-the-curve.global positioning system (gps) technology for monitoring home range and movements of wildlife has resulted in prohibitively large sample sizes of locations for traditional estimators of home range. we used area-under-the-curve to explore the fit of 8 estimators of home range to data collected with both gps and concurrent very high frequency (vhf) technology on a terrestrial mammal, the florida panther puma concolor coryi, to evaluate recently developed and traditional estimators.201525973204
ecological relationships of meso-scale distribution in 25 neotropical vertebrate species.vertebrates are a vital ecological component of amazon forest biodiversity. although vertebrates are a functionally important part of various ecosystem services they continue to be threatened by anthropogenic impacts throughout the amazon. here we use a standardized, regularly spaced arrangement of camera traps within 25km2 to provide a baseline assessment of vertebrate species diversity in a sustainable use protected area in the eastern brazilian amazon. we examined seasonal differences in the ...201525938582
using predator-prey theory to predict outcomes of broadscale experiments to reduce apparent competition.apparent competition is an important process influencing many ecological communities. we used predator-prey theory to predict outcomes of ecosystem experiments aimed at mitigating apparent competition by reducing primary prey. simulations predicted declines in secondary prey following reductions in primary prey because predators consumed more secondary prey until predator numbers responded to reduced prey densities. losses were exacerbated by a higher carrying capacity of primary prey and a long ...201525905509
evaluating landscape connectivity for puma concolor and panthera onca among atlantic forest protected areas.strictly protected areas and riparian forests in brazil are rarely large enough or connected enough to maintain viable populations of carnivores and animal movement over time, but these characteristics are fundamental for species conservation as they prevent the extinction of isolated animal populations. therefore, the need to maintain connectivity for these species in human-dominated atlantic landscapes is critical. in this study, we evaluated the landscape connectivity for large carnivores (co ...201525860593
do attacks by jaguars panthera onca and pumas puma concolor (carnivora: felidae) on livestock correlate with species richness and relative abundance of wild prey?abstract: attacks by big cats on livestock are one of the major causes of human-felid conflicts and, therefore, an important factor in the conservation of these species. it has been argued that a reduction in natural prey abundance promotes attacks on domestic species, but few studies have tested this statement, and some have delivered contradictory results. we investigated whether the occurrence of attacks to livestock by jaguar and puma relates to the abundance and richness of their natural pr ...201425720180
movement, resting, and attack behaviors of wild pumas are revealed by tri-axial accelerometer measurements.accelerometers are useful tools for biologists seeking to gain a deeper understanding of the daily behavior of cryptic species. we describe how we used gps and tri-axial accelerometer (sampling at 64 hz) collars to monitor behaviors of free-ranging pumas (puma concolor), which are difficult or impossible to observe in the wild. we attached collars to twelve pumas in the santa cruz mountains, ca from 2010-2012. by implementing random forest models, we classified behaviors in wild pumas based on t ...201525709837
high prevalence of trichinella pseudospiralis in florida panthers (puma concolor coryi).parasites of the genus trichinella are zoonotic nematodes common in carnivores throughout the world. we determined the prevalence and species of trichinella infections in florida panthers (puma concolor coryi).201525649739
malignant paraganglioma in a cougar (puma concolor).a 7½-yr-old male cougar (puma concolor) was presented with a 2-wk history of progressive hindlimb abnormalities. an abdominal mass was palpated on physical examination. computed tomography of the abdomen showed a mass surrounding the left ureter. a postmortem diagnosis of paraganglioma was established.201425632701
medicinal use of wild fauna by mestizo communities living near san guillermo biosphere reserve (san juan, argentina).wild and domestic animals and their by-products are important ingredients in the preparation of curative, protective and preventive medicines. despite the medicinal use of animals worldwide, this topic has received less attention than the use of medicinal plants. this study assessed the medicinal use of animals by mestizo communities living near san guillermo mab reserve by addressing the following questions: what animal species and body parts are used? what ailments or diseases are treated with ...201525608977
top carnivores increase their kill rates on prey as a response to human-induced fear.the fear induced by predators on their prey is well known to cause behavioural adjustments by prey that can ripple through food webs. little is known, however, about the analogous impacts of humans as perceived top predators on the foraging behaviour of carnivores. here, we investigate the influence of human-induced fear on puma foraging behaviour using location and prey consumption data from 30 tagged individuals living along a gradient of human development. we observed strong behavioural respo ...201525608884
attractiveness of native mammal's feces of different trophic guilds to dung beetles (coleoptera: scarabaeinae).mammal feces are the primary food and nesting resource for the majority of dung beetle species, and larval development depends on the quantity and quality of that resource. physiological necessities, competitive interactions, and resource sharing are common and suggest that dung beetles may show preferences for feces of greater nutritional quality, which may in turn impact beetle assemblages and community structure. this study investigated whether attractiveness of dung beetles to different reso ...201425528749
effect of bungee-carcass enrichment on behavior and fecal glucocorticoid metabolites in two species of zoo-housed felids.enrichment can improve animal physiological and psychological well-being. this study sought to promote more natural felid behavior and prevent development or incidence of stereotypies through the use of a feeding enrichment. our objectives are to use fecal glucocorticoid metabolites values and behavioral observations to quantify the effectiveness of the enrichment device for two species of large cats, bengal tiger (panthera tigris tigris; n = 2) and cougar (felis concolor; n = 2). the feeding en ...201525492384
habitat capacity for cougar recolonization in the upper great lakes region.recent findings indicate that cougars (puma concolor) are expanding their range into the midwestern united states. confirmed reports of cougar in michigan, minnesota, and wisconsin have increased dramatically in frequency during the last five years, leading to speculation that cougars may re-establish in the upper great lakes (ugl) region, usa. recent work showed favorable cougar habitat in northeastern minnesota, suggesting that the northern forested regions of michigan and wisconsin may have s ...201425389761
effects of hunting on cougar spatial organization.the effects of increased mortality on the spatial dynamics of solitary carnivores are not well understood. we examined the spatial ecology of two cougar populations that differed in hunting intensity to test whether increased mortality affected home range size and overlap. the stability hypothesis predicts that home range size and overlap will be similar for both sexes among the two areas. the instability hypothesis predicts that home range size and overlap will be greater in the heavily hunted ...201425360259
high consumption of primates by pumas and ocelots in a remnant of the brazilian atlantic forest.we studied the diet of the ocelot and puma during the years 2007 and 2008 at the feliciano miguel abdala reserve, in minas gerais, south-eastern brazil. we collected 49 faecal samples (scats) from cats, and identified the species of cat from 23 of them by the analysis of the microstructure patterns of hairs found in their faeces: 17 scats of the puma (puma concolor) and six of the ocelot (leopardus pardalis). in the puma scats, we identified three species of primates (brachyteles hypoxanthus, al ...201425296212
fractured genetic connectivity threatens a southern california puma (puma concolor) population.pumas (puma concolor; also known as mountain lions and cougars) in southern california live among a burgeoning human population of roughly 20 million people. yet little is known of the consequences of attendant habitat loss and fragmentation, and human-caused puma mortality to puma population viability and genetic diversity. we examined genetic status of pumas in coastal mountains within the peninsular ranges south of los angeles, in san diego, riverside, and orange counties. the santa ana mount ...201425295530
detection of antibodies to toxoplasma gondii in wild animals in brazil.toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite, toxoplasma gondii, that affects all warm-blooded animals, including wild animals. the increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. in this study, the occurrence of t. gondii infection was investigated by the modified agglutination test (mat) in 26 differ ...201425278979
mammalian energetics. instantaneous energetics of puma kills reveal advantage of felid sneak attacks.pumas (puma concolor) live in diverse, often rugged, complex habitats. the energy they expend for hunting must account for this complexity but is difficult to measure for this and other large, cryptic carnivores. we developed and deployed a physiological smart (species movement, acceleration, and radio tracking) collar that used accelerometry to continuously monitor energetics, movements, and behavior of free-ranging pumas. this felid species displayed marked individuality in predatory activitie ...201425278610
hidden semi-markov models reveal multiphasic movement of the endangered florida panther.animals must move to find food and mates, and to avoid predators; movement thus influences survival and reproduction, and ultimately determines fitness. precise description of movement and understanding of spatial and temporal patterns as well as relationships with intrinsic and extrinsic factors is important both for theoretical and applied reasons. we applied hidden semi-markov models (hsmm) to hourly geographic positioning system (gps) location data to understand movement patterns of the enda ...201525251870
using detection dogs and genetic analyses of scat to expand knowledge and assist felid conservation in misiones, argentina.many carnivores require large ranges to meet their ecological and energetic needs; however, anthropogenic changes threaten species and their habitats. camera traps have been used to effectively collect data on carnivores in a variety of habitat types; however, a single survey effort is typically limited to species that have similar body size, habitat use and movement patterns, and individual identification of animals is not always possible. we evaluated whether scat detection dogs could effectiv ...201425236691
resistance to antibiotics of clinical relevance in the fecal microbiota of mexican wildlife.there are a growing number of reports of antibiotic resistance (atbr) in bacteria living in wildlife. this is a cause for concern as atbr in wildlife represents a potential public health threat. however, little is known about the factors that might determine the presence, abundance and dispersion of atbr bacteria in wildlife. here, we used culture and molecular methods to assess atbr in bacteria in fecal samples from howler monkeys (alouatta palliata), spider monkeys (ateles geoffroyi), tapirs ( ...201425233089
individual behaviors dominate the dynamics of an urban mountain lion population isolated by roads.large carnivores can be particularly sensitive to the effects of habitat fragmentation on genetic diversity [1, 2]. the santa monica mountains (smms), a large natural area within greater los angeles, is completely isolated by urban development and the 101 freeway to the north. yet the smms support a population of mountain lions (puma concolor), a very rare example of a large carnivore persisting within the boundaries of a megacity. gps locations of radio-collared lions indicate that freeways are ...201425131676
susceptibility of chickens (gallus gallus domesticus) to trichinella patagoniensis.trichinella spp. is a genus of parasites that is widespread all over the world. in argentina, t. spiralis was for years the only species involved in human and animal outbreaks. during the last decade, t. patagoniensis, a new trichinella species, was discovered in argentina. up to now, this species has only been found in cougars (puma concolor). experimental infections in pigs, cats, mice and rats with this new genotype showed that cats and mice were the most susceptible hosts. the aim of the pre ...201425123612
trophic facilitation or limitation? comparative effects of pumas and black bears on the scavenger community.scavenging is a widespread behaviour and an important process influencing food webs and ecological communities. large carnivores facilitate the movement of energy across trophic levels through the scavenging and decomposition of their killed prey, but competition with large carnivores is also likely to constrain acquisition of carrion by scavengers. we used an experimental approach based on motion-triggered video cameras at black-tailed deer (odocoileus hemionus columbianus) carcasses to measure ...201425010629
the dynamic proliferation of cansines mirrors the complex evolution of feliforms.repetitive short interspersed elements (sines) are retrotransposons ubiquitous in mammalian genomes and are highly informative markers to identify species and phylogenetic associations. of these, sines unique to the order carnivora (cansines) yield novel insights on genome evolution in domestic dogs and cats, but less is known about their role in related carnivores. in particular, genome-wide assessment of cansine evolution has yet to be completed across the feliformia (cat-like) suborder of car ...201424947429
what makes a feline fatal in toxoplasma gondii's fatal feline attraction? infected rats choose wild cats.toxoplasma gondii is an indirectly transmitted protozoan parasite, of which members of the cat family (felidae) are the only definitive hosts and small mammals such as rats serve as intermediate hosts. the innate aversion of rodents to cat odor provides an obstacle for the parasite against successful predation by the feline definitive host. previous research has demonstrated that t. gondii appears to alter a rat's perception of the risk of being preyed upon by cats. although uninfected rats disp ...201424907200
cougars' key to survival through the late pleistocene extinction: insights from dental microwear texture analysis.cougars (puma concolor) are one of only two large cats in north america to have survived the late pleistocene extinction (lpe), yet the specific key(s) to their relative success remains unknown. here, we compare the dental microwear textures of pleistocene cougars with sympatric felids from the la brea tar pits in southern california that went extinct at the lpe (panthera atrox and smilodon fatalis), to clarify potential dietary factors that led to the cougar's persistence through the lpe. we fu ...201424759373
noninvasive individual and species identification of jaguars (panthera onca), pumas (puma concolor) and ocelots (leopardus pardalis) in belize, central america using cross-species microsatellites and faecal dna.there is a great need to develop efficient, noninvasive genetic sampling methods to study wild populations of multiple, co-occurring, threatened felids. this is especially important for molecular scatology studies occurring in challenging tropical environments where dna degrades quickly and the quality of faecal samples varies greatly. we optimized 14 polymorphic microsatellite loci for jaguars (panthera onca), pumas (puma concolor) and ocelots (leopardus pardalis) and assessed their utility for ...201424751217
evolution of puma lentivirus in bobcats (lynx rufus) and mountain lions (puma concolor) in north america.mountain lions (puma concolor) throughout north and south america are infected with puma lentivirus clade b (plvb). a second, highly divergent lentiviral clade, plva, infects mountain lions in southern california and florida. bobcats (lynx rufus) in these two geographic regions are also infected with plva, and to date, this is the only strain of lentivirus identified in bobcats. we sequenced full-length plv genomes in order to characterize the molecular evolution of plv in bobcats and mountain l ...201424741092
analytical methods for chemical and sensory characterization of scent-markings in large wild mammals: a review.in conjoining the disciplines of "ethology" and "chemistry" the field of "ethochemistry" has been instituted. ethochemistry is an effective tool in conservation efforts of endangered species and the understanding of behavioral patterns across all species. chemical constituents of scent-markings have an important, yet poorly understood function in territoriality, reproduction, dominance, and impact on evolutionary biology, especially in large mammals. particular attention has recently been focuse ...201424603639
novel gammaherpesviruses in north american domestic cats, bobcats, and pumas: identification, prevalence, and risk factors.gammaherpesviruses (ghvs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. to identify felid ghvs, we screened domestic cat (felis catus), bobcat (lynx rufus), and puma (puma concolor) blood cell dna samples from california, colorado, and florida using a degenerate pan-ghv pcr. additional pan-ghv and long-distance pcrs were used to sequence a contiguous 3.4-kb region of each putative virus species, including partial glyco ...201424453374
intralesional vincristine use for treatment of squamous cell carcinoma in a puma (puma concolor).a 14-yr-old male puma (puma concolor) was presented to the veterinary staff of the national zoo in malaysia for an auricular mass. squamous cell carcinoma was diagnosed by histologic examination of a biopsy. systemic administration of chemotherapy using vincristine (0.5 mg/m2 i.v. q. 7 days for six treatments) and prednisolone (2 mg/kg i.m. q. 72 hr x 7 days) caused side effects of vomiting, weight loss, and alopecia and did not improve the size or appearance of the tumor. intralesional vincrist ...201324450069
cross transmission of gastrointestinal nematodes between captive neotropical felids and feral cats.pathogen cross transmission between wildlife and domestic animals represents an extinction risk for wildlife; however, reliable verification is difficult to perform, and in some cases, it is even considered unlikely to be conducted. the aim of this work was to identify cross transmission of helminths between feral cats and captive wild felids at a zoological park in southeastern mexico. feces were collected from jaguars (panthera onca), cougars (puma concolor), ocelots (leopardus pardalis), marg ...201324450052
molecular evidence for a recent demographic expansion in the puma (puma concolor) (mammalia, felidae).the puma is an iconic predator that ranges throughout the americas, occupying diverse habitats. previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. moreover, most of the species' molecular diversity was found to be in south america. to further investigate the phylogeographic structure and demographic history of pumas we analyzed mtdn ...201324385863
models of regional habitat quality and connectivity for pumas (puma concolor) in the southwestern united states.the impact of landscape changes on the quality and connectivity of habitats for multiple wildlife species is of global conservation concern. in the southwestern united states, pumas (puma concolor) are a well distributed and wide-ranging large carnivore that are sensitive to loss of habitat and to the disruption of pathways that connect their populations. we used an expert-based approach to define and derive variables hypothesized to influence the quality, location, and permeability of habitat f ...201324367495
seasonal foraging ecology of non-migratory cougars in a system with migrating prey.we tested for seasonal differences in cougar (puma concolor) foraging behaviors in the southern yellowstone ecosystem, a multi-prey system in which ungulate prey migrate, and cougars do not. we recorded 411 winter prey and 239 summer prey killed by 28 female and 10 male cougars, and an additional 37 prey items by unmarked cougars. deer composed 42.4% of summer cougar diets but only 7.2% of winter diets. males and females, however, selected different proportions of different prey; male cougars se ...201324349498
leukoencephalomyelopathy of mature captive cheetahs and other large felids: a novel neurodegenerative disease that came and went?a novel leukoencephalomyelopathy was identified in 73 mature male and female large captive felids between 1994 and 2005. while the majority of identified cases occurred in cheetahs (acinonyx jubatus), the disease was also found in members of 2 other subfamilies of felidae: 1 generic tiger (panthera tigris) and 2 florida panthers (puma concolor coryi). the median age at time of death was 12 years, and all but 1 cheetah were housed in the united states. characteristic clinical history included pro ...201424129896
seroprevalence of toxoplasma gondii infection in captive mammals in three zoos in mexico city, mexico.antibodies to toxoplasma gondii were determined in 167 mammals in three zoos in mexico city, mexico, using the modified agglutination test (mat). overall, antibodies to t. gondii were found in 89 (53.3%) of the 167 animals tested. antibodies were found in 35 of 43 wild felidae: 2 of 2 bobcats (lynx rufus); 4 of 4 cougars (puma concolor); 10 of 13 jaguars (panthera onca); 5 of 5 leopards (panthera pardus); 7 of 7 lions (panthera leo); 2 of 3 tigers (panthera tigris); 2 of 3 ocelots (leopardus par ...201324063119
does sex matter? temporal and spatial patterns of cougar-human conflict in british columbia.wildlife-human conflicts occur wherever large carnivores overlap human inhabited areas. conflict mitigation can be facilitated by understanding long-term dynamics and examining sex-structured conflict patterns. predicting areas with high probability of conflict helps focus management strategies in order to proactively decrease carnivore mortality. we investigated the importance of cougar (puma concolor) habitat, human landscape characteristics and the combination of habitat and human features on ...201324040312
a new atypical genotype mouse virulent strain of toxoplasma gondii isolated from the heart of a wild caught puma (felis concolor) from durango, mexico.nothing is known of the genetic diversity of toxoplasma gondii circulating in wildlife in mexico. in the present study, a mouse virulent t. gondii strain was isolated from the heart of a wild puma (felis concolor). the puma was found roaming in outskirt of durango city, mexico and tranquilized for moving to a zoo. the puma died during translocation and a necropsy examination was performed. the puma had an antibody titer for t. gondii of 200 by the modified agglutination test. its heart and brain ...201323849518
mitochondrial dna sequence variation and phylogeography of neotropic pumas (puma concolor).pumas occupy the largest latitudinal range of any new world terrestrial mammal. human population growth and associated habitat reduction has reduced their north american range by nearly two-thirds, but the impact of human expansion in central and south america on puma populations is not clear. we examined mitochondrial dna diversity of pumas across the majority of their range, with a focus on central and south america. four mitochondrial gene regions (1140 base pairs) revealed 16 unique haplotyp ...201423789770
characterization of regionally associated feline immunodeficiency virus (fiv) in bobcats (lynx rufus).feline immunodeficiency virus (fiv) classically infects felid species with highly divergent species-specific fivs. however, recent studies have detected an fiv strain infecting both bobcats (lynx rufus) and pumas (puma concolor) in california and florida. to further investigate this observation, we evaluated fiv from bobcats in florida (n=25) and colorado (n=80) between 2008 and 2011. partial viral sequences from five florida bobcats cluster with previously published sequences from florida panth ...201323778629
values, animal symbolism, and human-animal relationships associated to two threatened felids in mapuche and chilean local narratives.the chilean temperate rainforest has been subjected to dramatic fragmentation for agriculture and forestry exploitation. carnivore species are particularly affected by fragmentation and the resulting resource use conflicts with humans. this study aimed at understanding values and human-animal relationships with negatively perceived threatened carnivores through the disclosure of local stories and mapuche traditional folktales.201323764186
scale dependent behavioral responses to human development by a large predator, the puma.the spatial scale at which organisms respond to human activity can affect both ecological function and conservation planning. yet little is known regarding the spatial scale at which distinct behaviors related to reproduction and survival are impacted by human interference. here we provide a novel approach to estimating the spatial scale at which a top predator, the puma (puma concolor), responds to human development when it is moving, feeding, communicating, and denning. we find that reproducti ...201323613732
feline infectious peritonitis in a mountain lion (puma concolor), california, usa.feline infectious peritonitis (fip) is a fatal immune-mediated vasculitis of felids caused by a mutant form of a common feline enteric virus, feline enteric coronavirus. the virus can attack many organ systems and causes a broad range of signs, commonly including weight loss and fever. regardless of presentation, fip is ultimately fatal and often presents a diagnostic challenge. in may 2010, a malnourished young adult male mountain lion (puma concolor) from kern county, california, usa was eutha ...201323568918
development and validation of a fecal pcr assay for notoedres cati and application to notoedric mange cases in bobcats (lynx rufus) in northern california, usa.notoedric mange in felids is a devastating disease caused by a hypersensitivity reaction to the mite notoedres cati. the burrowing of the mite causes intense pruritis resulting in self-mutilation, secondary bacterial infection, and often death of affected felids if left untreated. our understanding of how notoedric mange is maintained in felid populations, and the true geographic extent of infestations, has been hampered because wild felids are elusive and, thus, traditional diagnostic methods a ...201323568905
risk factors for exposure to feline pathogens in california mountain lions (puma concolor).the primary challenge to mountain lion population viability in california is habitat loss and fragmentation. these habitat impacts could enhance disease risk by increasing contact with domestic animals and by altering patterns of exposure to other wild felids. we performed a serologic survey for feline pathogens in california mountain lions (puma concolor) using 490 samples from 45 counties collected from 1990 to 2008. most mountain lions sampled were killed because of depredation or public safe ...201323568903
complete genome sequences of two novel puma concolor foamy viruses from california.we report two complete foamy retrovirus (fv) genomes isolated from puma concolor, a large cat native to the americas. due to high overall genetic relatedness to known feline foamy viruses (ffvs), we propose the name puma concolor ffv (ffvpc). the data confirm that felines are infected with distinct but closely related fvs.201323516229
experimental infection with trichinella t12 in domestic cats.trichinella spiralis has been documented in wild animals in argentina, including puma, armadillos, rats and wild boars. in 2008, molecular analysis identified trichinella t12 from a naturally infected puma (puma concolor) from patagonia. the aim of the present work was to study the relationship between the infectivity and pathology of trichinella t12 in the puma and in domestic cats, and the possible risks that may be present for transmission among these animals. two cats (a and b) were orally-i ...201323474230
where do you come from, where do you go? directional migration rates in landscape genetics.identifying landscape elements that influence gene flow and migration in wild species is the current main topic of landscape genetics. most landscape genetic studies infer gene flow and migration from genetic distances among populations or individuals and statistically relate these measurements to landscape composition and configuration. this approach assumes symmetrical gene flow between pairs of populations. such an assumption, however, will often be violated, especially in source–sink systems ...201223310963
nuisance ecology: do scavenging condors exact foraging costs on pumas in patagonia?predation risk describes the energetic cost an animal suffers when making a trade off between maximizing energy intake and minimizing threats to its survival. we tested whether andean condors (vultur gryphus) influenced the foraging behaviors of a top predator in patagonia, the puma (puma concolor), in ways comparable to direct risks of predation for prey to address three questions: 1) do condors exact a foraging cost on pumas?; 2) if so, do pumas exhibit behaviors indicative of these risks?; an ...201323301093
high proportion of male faeces in jaguar populations.faeces provide relevant biological information which includes, with the application of genetic techniques, the sex and identity of individuals that defecated, thus providing potentially useful data on the behaviour and ecology of individuals, as well as the dynamics and structure of populations. this paper presents estimates of the sex ratio of different felid species (jaguar, panthera onca; puma, puma concolor; and ocelot/margay, leopardus pardalis/leopardus wiedi) as observed in field collecte ...201223285226
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