Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| molecular characterization and gene expression of japanese eel (anguilla japonica) gonadotropin receptors. | a luteinizing hormone receptor (lhr) cdna with high identity to other fish lhrs was fully cloned from the ovary of the japanese eel (anguilla japonica). the genes for two gonadotropin receptors (gthr), follicle-stimulating hormone receptor (fshr) and lhr, were differentially expressed during oogenesis, which was artificially induced by salmon pituitary extract, a gonadotropin-rich source. transcript abundance of fshr was significantly elevated at the early vitellogenic stage and peaked at the la ... | 2012 | 22379989 |
| immunogenicity of pressure inactivated edwardsiella tarda bacterin to anguilla japonica (japanese eel). | japanese eel anguilla japonica were immunized with inactivated edwardsiella tarda bacterin preparations (formalin-killed cells, fkc (0.4%), formalin with heat-killed cells, fhkc (0.1% and 70 degreesc for 10 min), heat-killed cells, hkc (70 degrees c for 15 min), potassium chloride-killed cells, kkc (0.6%), tannic acid-killed cells, tkc (0.9%), citric acid-killed cells, cakc (0.9%), pressure-killed cells, pkc (600 psi for 5 min) and electric current-killed cells, eckc (100 ma at 12 v dc for 5 sec ... | 2011 | 22303581 |
| differential regulation of the expression of cytochrome p450 aromatase, estrogen and androgen receptor subtypes in the brain-pituitary-ovarian axis of the japanese eel (anguilla japonica) reveals steroid dependent and independent mechanisms. | this study aimed at investigating the role of sexual steroids in the regulation of the expression of the single aromatase gene and steroid receptor subtypes in the brain-pituitary-ovarian axis of the japanese eel. unlike other teleosts, which possess duplicated genes for aromatase, cyp19a1a and cyp19a1b, expressed in the gonads and in the brain, respectively, eel species possess a single cyp19a1. phylogenetic analysis indicated that eel brain/gonadal cyp19a1 branches at the basis of both teleost ... | 2012 | 22107840 |
| efficiency of peptide nucleic acid-directed pcr clamping and its application in the investigation of natural diets of the japanese eel leptocephali. | polymerase chain reaction (pcr)-clamping using blocking primer and dna-analogs, such as peptide nucleotide acid (pna), may be used to selectively amplify target dna for molecular diet analysis. we investigated pcr-clamping efficiency by studying pna position and mismatch with complementary dna by designing pnas at five different positions on the nuclear rdna internal transcribed spacer 1 of the japanese eel anguilla japonica in association with intra-specific nucleotide substitutions. all five p ... | 2011 | 22069444 |
| testosterone improves the transition of primary oocytes in artificial maturation eels (anguilla japonica) by altering ovarian pten expression. | in mammals, androgens appear to enhance the development of primary ovarian follicles, but pi3k (phosphoinositide 3-kinases) pathway is well recognized as one of the critical pathways in early follicular development. roles of the pi3k were revealed by deletion of pten (phosphatase and tensin homolog on chromosome 10). pten is demonstrated to play an important role in the early stage of follicle development. in the japanese eel, two forms of pten have been cloned, but what their functions on the d ... | 2012 | 21986810 |
| gh is produced by the testis of japanese eel and stimulates proliferation of spermatogonia. | gh plays important roles in development, somatic growth and gametogenesis in vertebrates. to determine the physiological role of gh in reproduction in male teleosts, the expression of genes encoding gh and the two gh receptors (ghrs) during spermatogenesis, and the action of gh in vitro was examined using the japanese eel (anguilla japonica). gh, ghr1 and ghr2 mrna transcripts were detected in all spermatogenic stages. in situ hybridization showed the presence of ghr1 and ghr2 mrna in the germ c ... | 2011 | 21976616 |
| the mechanism for primordial germ-cell migration is conserved between japanese eel and zebrafish. | primordial germ cells (pgcs) are segregated and specified from somatic cells during early development. these cells arise elsewhere and have to migrate across the embryo to reach developing gonadal precursors. several molecules associated with pgc migration (i.e. dead-end, nanos1, and cxcr4) are highly conserved across phylum boundaries. however, since cell migration is a complicated process that is regulated spatially and temporally by multiple adaptors and signal effectors, the process is unlik ... | 2011 | 21931724 |
| behavioral migration diversity of the yangtze river japanese eel, anguilla japonica, based on otolith sr/ca ratios. | the yangtze river estuary is the main production area of anguilla japonica in china, as well as the only existing fishery area for adult eels. japanese eels are distributed in the main rivers and many tributaries from the yangtze river estuary to the upper jinsha river, which extend to nearly 3 000 km. however, their migration behaviors remain relatively unknown. we analyzed the biological characteristics of 153 specimens of silver eels collected from the jingjiang section of yangtze river (31(o ... | 2011 | 21842541 |
| distribution of pepsinogen- and ghrelin-producing cells in the digestive tract of japanese eel (anguilla japonica) during metamorphosis and the adult stage. | pepsinogen is the precursor form of the gastric-specific digestive enzyme, pepsin. ghrelin is a representative gastric hormone with multiple functions in vertebrates, including the regulation of growth hormone release, stimulation of food intake and gastrointestinal motility function. we investigated chronological changes in the distribution of pepsinogen-expressing cells by in situ hybridization and ghrelin-immunoreactive cells by immunohistochemistry in the japanese eel (anguilla japonica) dur ... | 2011 | 21827762 |
| hatching enzyme of japanese eel anguilla japonica and the possible evolution of the egg envelope digestion mechanism. | we purified eel hatching enzyme (ehe) from the hatching liquid of japanese eel anguilla japonica belonging to elopomorpha to a single band on sds/page. tof-ms analysis revealed that the purified ehe contained several isozymes with similar molecular masses. comparison of the egg envelope digestion specificities of the purified ehe and of recombinant ehe4, one of the ehe isozymes, suggested that the isozymes contained in the purified ehe were functionally the same in terms of egg envelope digestio ... | 2011 | 21819542 |
| isolation and identification of a novel inducible antibacterial peptide from the skin mucus of japanese eel, anguilla japonica. | in this study, acetone extracts and acidic extracts were prepared from skin mucus, gill, kidney, liver and spleen of the japanese eel, anguilla japonica, and they exhibited different levels of antibacterial activities against three strains of gram-negative bacteria, edwardsiella tarda, aeromonas hydrophila, aeromonas sp. and one gram-positive bacterium micrococcus leteus. the mucus was chosen as the source of antibacterial peptide for further purification of antibacterial peptides. following the ... | 2011 | 21796440 |
| host size- and habitat-dependent intensity of heliconema longissimum (nematoda: physalopteridae) in the japanese eel (anguilla japonica). | heliconema longissimum (ortlepp, 1923) is an ecologically poorly known nematode found in the stomach of japanese eels, anguilla japonica temminck and schlegel. the occurrence of this nematode in japanese eels was surveyed in 2 contiguous brackish-water areas (misho cove and the lower renjoji river) of ehime prefecture, western japan, during april 2008 to march 2009. the factors associated with the nematode intensity were also assessed by applying generalized additive models (gam). heliconema lon ... | 2011 | 21671717 |
| expression and localization of aquaporin 1b during oocyte development in the japanese eel (anguilla japonica). | to elucidate the molecular mechanisms underling hydration during oocyte maturation, we characterized the structure of japanese eel (anguilla japonica) novel-water selective aquaporin 1 (aqp1b) that thought to be involved in oocyte hydration. the aqp1b cdna encodes a 263 amino acid protein that includes the six potential transmembrane domains and two asn-pro-ala motifs. reverse transcription-polymerase chain reaction showed transcription of japanese eel aqp1b in ovary and testis but not in the ot ... | 2011 | 21615964 |
| electroneutral cation-cl(-) cotransporters nkcc2β and nccβ expressed in the intestinal tract of japanese eel anguilla japonica. | in the present study, we aimed to elucidate the mechanisms of intestinal na(+) and cl(-) absorption in japanese eel, focusing on electroneutral cation-cl(-) cotransporters, nkcc2β and nccβ, expressed in the intestinal tract. first, we cloned cdnas encoding nkcc2β and nccβ from the intestinal tract of japanese eel. in both freshwater- and seawater-acclimated eels, quantitative pcr analysis showed that nkcc2β was predominantly expressed in the anterior and posterior intestines, and that nccβ expre ... | 2011 | 21539929 |
| localization of diversified relaxin gene transcripts in the brain of eels. | relaxin 3 (rln3) is a newly-discovered member of the insulin superfamily. we isolated three rln3-like cdnas from the brain of the japanese eel (anguilla japonica). the deduced amino acid sequences of the rln3-like cdnas contained the two-chain structure common to relaxin including a rxxxrxxi/v motif in the b-chain. phylogenetic analysis assigned the two prepropeptides into teleost/mammalian rln3 group, which are a pair of duplicates generated by the teleost-specific third-round whole genome dupl ... | 2011 | 21530530 |
| offshore spawning of conger myriaster in the western north pacific: evidence for convergent migration strategies of anguilliform eels in the atlantic and pacific. | the spawning area of the common japanese conger, conger myriaster, had remained unknown because spawning adults or its newly hatched larvae were never collected. using genetic identification, we determined that c. myriaster spawns far offshore in the western north pacific, just west of the spawning area of the japanese eel, anguilla japonica. in june 2008, six newly hatched c. myriaster larvae, 5.6-6.9 mm, were collected at the eastern edge of where many small unidentified conger leptocephali (7 ... | 2011 | 21509576 |
| modulation of ion transporter expression in gill mitochondrion-rich cells of eels acclimated to low-na(+) or-cl(-) freshwater. | in this study, we aimed to establish an experimental model to study the role of the gill mitochondrion-rich cells (mrcs) of freshwater fish in na(+) uptake and to examine the effect of adjusting external na(+) and cl(-) ions on selected ion transporters in gill mrcs. japanese eels (anguilla japonica) acclimated to deionized (di) water for 2 weeks were transferred directly to (a) ion-supplemented artificial freshwater (af), (b) na(+) -deficient af, or (c) cl(-) -deficient af for 2 days. the effec ... | 2011 | 21455947 |
| [seasonal changes of fish species composition and diversity in mudflat wetlands of hangzhou bay]. | in order to understand the spatiotemporal variation of fish species composition and biodiversity in the mudflat wetlands of hangzhou bay, thirty six surveys were conducted in the mudflat area, inning area, and aquaculture area in the south bank of the bay in. march (early spring), may (spring), july (summer), and october (autumn), 2009. a total of 41 species belonging to 9 orders and 16 families were observed, among which, cyprinid had the largest species number (14 species, 33.3% of the total), ... | 2010 | 21443016 |
| ovarian steroidogenesis and the role of sex steroid hormones on ovarian growth and maturation of the japanese eel. | three sex steroid hormones, estradiol-17β (e2), 11-ketotestosterone (11-kt), and 17α,20β-dihydroxy-4-pregnen-3-one (dhp), are well established as primary estrogen, androgen, and progestin, respectively, in teleost fish. japanese eel, anguilla japonica, would be a suitable candidate to study ovarian steroid physiology of fish because the ovarian growth and steroidogenesis is dormant under laboratory condition but can be induced by administration of exogenous gonadotropic reagents. in this review, ... | 2011 | 21414407 |
| first artificial hybrid of the eel species anguilla australis and anguilla anguilla. | studies on artificial hybridization of different anguilla species were conducted recently, i.e. female a. australis with male a. dieffenbachii, and female a. japonica with male a. anguilla. the existence of these artificial hybrids was however not demonstrated by independent genetic methods. two species - a. anguilla and a. australis - that are phylogenetically close but have different sexual maturation times (12-25 weeks and 6-8 weeks, respectively), were expected to produce favourable hybrids ... | 2011 | 21396126 |
| dynamics of reproductive hormones during downstream migration in females of the japanese eel, anguilla japonica. | the profiles of sex steroids (estradiol-17β, testosterone and 11-ketotestosterone) and the mrna levels of gonadotropins (luteinizing hormone and follicle-stimulating hormone) were investigated before and after downstream migration in females of the japanese eel species anguilla japonica, which were collected in the brackish hamana lake and its inlet freshwater rivers. eels were separated into three groups using otolith microchemistry: 'migrants' that grew in the inlet rivers and then made a down ... | 2011 | 21385058 |
| novel dna virus isolated from samples showing endothelial cell necrosis in the japanese eel, anguilla japonica. | economic loss due to viral endothelial cell necrosis of eel (vecne) of anguilla japonica is a serious problem for the cultured japanese eel market. however, the viral genome responsible for vecne is unknown. we recently developed a rapid determination system for viral nucleic acid sequences (rdv) to determine viral genome sequences. in this study, viral dna fragments were obtained using rdv, and approximately 15-kbp circular full genome sequences were determined using a next-generation sequencin ... | 2011 | 21277610 |
| androgens and very low density lipoprotein are essential for the growth of previtellogenic oocytes from japanese eel, anguilla japonica, in vitro. | to investigate the regulation of lipid uptake into the eel oocyte in more detail, effects of 11-ketotestosterone (11-kt) and lipid transporters (lipoproteins) were determined in vitro. ovarian explants from previtellogenic japanese eels (anguilla japonica) were incubated for 28 days with 11-kt and/or with very low density lipoproteins (vldl), low density lipoproteins (ldl), or high density lipoproteins (hdl) purified from eel plasma. the androgen 11-kt induced notable increases in oocyte diamete ... | 2011 | 21148104 |
| potent cardiovascular effects of homologous urotensin ii (uii)-related peptide and uii in unanesthetized eels after peripheral and central injections. | we cloned cdnas encoding urotensin ii (uii)-related peptide (urp) and uii in japanese eel, anguilla japonica, the former being the first such cloning in teleost fishes. unlike the exclusive expression of uii in the urophysis, the urp gene was expressed most abundantly in the brain (medulla oblongata) followed by the urophysis. peripheral injections of urp into eels increased blood pressure by 16.1 ± 0.8 mmhg at 0.1 nmol/kg in ventral aortic blood pressure (p(va)) and with similar potency and eff ... | 2011 | 21123764 |
| effects of extracellular environment factors on the motility in japanese eel spermatozoa. | we have examined the effects in vitro of the ionic composition, ph and temperature on the motility by the spermatozoa of the japanese eel (anguilla japonica). milt was obtained from 10 males that had been artificially matured by repeated injections of hcg. sperm motility was monitored with a vhs video recorder and a video camera connected to a microscope. the results showed that most of the sperm were highly motile in 250-700 mm nacl, 250-650 mm kcl and 350-550 mm cacl2 solution. the longest dur ... | 2011 | 20979577 |
| evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the japanese eel, anguilla japonica. | a fish egg envelope is composed of several glycoproteins, called zona pellucida (zp) proteins, which are conserved among vertebrate species. euteleost fishes synthesize zp proteins in the liver, while otocephalans synthesize them in the growing oocyte. we investigated zp proteins of the japanese eel, anguilla japonica, belonging to elopomorpha, which diverged earlier than euteleostei and otocephala. five major components of the egg envelope were purified and their partial amino acid sequences we ... | 2010 | 20977667 |
| modelling the growth of japanese eel anguilla japonica in the lower reach of the kao-ping river, southern taiwan: an information theory approach. | information theory was applied to select the best model fitting total length (l(t))-at-age data and calculate the averaged model for japanese eel anguilla japonica compiled from published literature and the differences in growth between sexes were examined. five candidate growth models were the von bertalanffy, generalized von bertalanffy, gompertz, logistic and power models. the von bertalanffy growth model with sex-specific coefficients was best supported by the data and nearly overlapped the ... | 2009 | 20738485 |
| salinity-linked growth in anguillid eels and the paradox of temperate-zone catadromy. | temperate-zone anguillid eels use both saline (marine or brackish) and fresh waters during their continental phase, but use of fresh waters is paradoxical because on average these fishes grow more rapidly in saline than in fresh waters. based on data from anguillid eels whose habitat-residency histories had been determined by sr:ca otolithometry, superiority of growth rates in saline water is much greater in american eels anguilla rostrata in north-eastern north america (mean saline:fresh growth ... | 2009 | 20735690 |
| influence of water temperature and feeding regime on otolith growth in anguilla japonica glass eels and elvers: does otolith growth cease at low temperatures? | the influences of water temperature and feeding regime on otolith growth in anguilla japonica glass eels and elvers were investigated using individuals reared at 5, 10, 15, 20, 25 and 30 degrees c and in fed or unfed conditions at salinity 32 after their otoliths were marked with alizarin complexone (alc). to eliminate the difficulty of observing the edges of otoliths with optical (om) or scanning electron (sem) microscopes, three to 10 individuals were sampled from each tank at 10, 20 and 30 da ... | 2009 | 20735680 |
| the effects of seasonal and interannual variability of oceanic structure in the western pacific north equatorial current on larval transport of the japanese eel anguilla japonica. | as the north equatorial current (nec)-bifurcation is known to be related to el niño-southern oscillation (enso) events, the influence of the position of the nec bifurcation on transport success of the larval japanese eel anguilla japonica was investigated. using a lagrangian modelling approach, larval transport was simulated and the relative influence of el niño and la niña events and the nec-bifurcation position on the success of particle transport analysed. the number of particles transported ... | 2009 | 20735678 |
| sympatric spawning of anguilla marmorata and anguilla japonica in the western north pacific ocean. | extensive collections were made of the larvae of the temperate japanese eel anguilla japonica and the tropical giant mottled eel anguilla marmorata in an overlapping area of the north equatorial current region of the western north pacific ocean. collections of 189 a. marmorata and > 2500 a. japonica larvae during nine surveys from 1991 to 2007 showed that these two anguillid eels have similar spawning areas just west of the southern west mariana ridge. in july to august 2006 and august 2007, mor ... | 2009 | 20735676 |
| oceanic migration and spawning of anguillid eels. | many aspects of the life histories of anguillid eels have been revealed in recent decades, but the spawning migrations of their silver eels in the open ocean still remains poorly understood. this paper overviews what is known about the migration and spawning of anguillid species in the ocean. the factors that determine exactly when anguillid eels will begin their migrations are not known, although environmental influences such as lunar cycle, rainfall and river discharge seem to affect their pat ... | 2009 | 20735675 |
| characterization of edwardsiella tarda isolated from farm-cultured eels, anguilla japonica, in the republic of korea. | we surveyed the occurrence of edwardsiellosis on eel farms and investigated the characteristics of edwardsiella tarda isolated from farm-cultured eels in the republic of korea. the occurrence rate of edwardsiellosis was 72% in the investigated samples. among the edwardsiellosis cases, 46% were found to be mixed infections, with parasites and other kinds of bacteria. some of the biochemical characteristics of the e. tarda isolates were different from those of the previously reported e. tarda isol ... | 2011 | 20716861 |
| the efficacy of five avirulent edwardsiella tarda strains in a live vaccine against edwardsiellosis in japanese flounder, paralichthys olivaceus. | we evaluated the tissue persistence and live vaccine efficacy of five avirulent edwardsiella tarda strains (e22, su100, su117, su138, and su244) isolated from the japanese eel (anguilla japonica) and from the environment. the live vaccines, containing a single strain, were injected intraperitoneally into japanese flounder (paralichthys olivaceus). viable bacteria from all the strains (excluding su100) were recovered from trunk-kidney tissue 28 d post-injection. japanese flounder inoculated with ... | 2010 | 20633656 |
| expression of androgen receptor mrna in the ovary of japanese eel, anguilla japonica, during artificially induced ovarian development. | in order to elucidate how androgens may mediate their effects on ovarian growth, we investigated the mrna levels of two subtypes of androgen receptor (ara and arb) in the ovary of feminized japanese eel (anguilla japonica) during artificially induced ovarian development by quantitative real-time reverse transcriptase polymerase chain reaction and in situ hybridization. ara mrna levels were high from the late oil droplet stage to the late vitellogenic stage, whereas arb mrna levels were high from ... | 2010 | 20553719 |
| investigation on natural diets of larval marine animals using peptide nucleic acid-directed polymerase chain reaction clamping. | the stomach contents of the larvae of marine animals are usually very small in quantity and amorphous, especially in invertebrates, making morphological methods of identification very difficult. nucleotide sequence analysis using polymerase chain reaction (pcr) is a likely approach, but the large quantity of larval (host) dna present may mask subtle signals from the prey genome. we have adopted peptide nucleic acid (pna)-directed pcr clamping to selectively inhibit amplification of host dna for ... | 2011 | 20535520 |
| withdrawn: molecular cloning and expression analysis of relaxin 3c in japanese eel, anguilla japonica. | this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2010 | 20233608 |
| [non-indigenous and non-specific parasitic nematodes--the background and the consequences]. | introduction of the host is the main reason behind introduction of the parasite, e. g., nematodes. the introduction of the japanese eel (anguilla japonica) in europe in the 1980s resulted in expansion of the swimbladder nematode anguillicola crassus which soon invaded not only populations of the european eel (a. anguilla), but also other local fish. the raccoon (procyon lotor) is native to north america. it is the specific host of strongyloides procyonis and baylisascaris procyonis. more than 90 ... | 2009 | 20209803 |
| production of recombinant japanese eel gonadotropins by baculovirus in silkworm larvae. | recombinant follicle-stimulating hormone (refsh) and luteinizing hormone (relh) of the japanese eel anguilla japonica were produced by baculovirus in silkworm bombyx mori larvae. cdnas encoding japanese eel gonadotropin subunits (i.e., fsh beta, lh beta, and common alpha) were introduced into the baculovirus, which was infected into silkworm larvae after propagation of the recombinant virus in b. mori culture cells. a 100ml solution of pooled hemolymph from silkworm larvae containing refsh or re ... | 2010 | 20064515 |
| investigating expression profiles of vegf-flk, and angpt1 during development of gas glands in japanese eel (anguilla japonica). | angiogenesis is a highly regulated physiological process in animals. angiopoietin-1 (angpt1) induces the signaling pathways related to vessel maturation in late phase of angiogenesis, which recruits pericyte supplements to make compact interaction with vessel tubes. there are only few data showing angpt1 functions in fish. by using degenerate primers, partial sequence (812 bp) of angpt1 was cloned from anguilla japonica, and deduced amino acids showed 80% similarity to those of zebrafish. physio ... | 2010 | 19962446 |
| massive encapsulation of larval anguillicoloides crassus in the intestinal wall of japanese eels. | abstract: | 2009 | 19832983 |
| cloning and regulation of expression of the na+-cl--taurine transporter in gill cells of freshwater japanese eels. | our previous studies have demonstrated the hypertonic-induced expression of osmotic stress transcription factor and the regulatory volume increase (rvi) response in gill cells isolated from freshwater eels. in this study, we aimed to clone one of the organic osmolyte transporters, the na+-cl--taurine transporter (taut), and to characterize its expression in anisosmotic conditions, using both in vivo and in vitro approaches. a cdna clone encoding taut was isolated from gill tissues of japanese ee ... | 2009 | 19801424 |
| hox genes of the japanese eel anguilla japonica and hox cluster evolution in teleosts. | compared with other diploid teleosts (2n=48), anguilloid fish have a specialized karyotype (2n=38) and remarkable morphological variation, and represent one basal group species of teleosts. to investigate the hox gene/cluster inventory in basal teleosts, a pcr-based survey of hox genes in the japanese eel (anguilla japonica) was conducted with both gene-specific and homeobox-targeted degenerate primers. our data provide evidence that at least 34 distinct hox genes exist in the japanese eel genom ... | 2010 | 19670462 |
| oocyte hydration in the japanese eel (anguilla japonica) during meiosis resumption and ovulation. | in this study, to elucidate the mechanisms of oocyte hydration in the japanese eel (anguilla japonica), we examined the in vivo and in vitro morphological changes and hydration process occurring during oocyte maturation and ovulation. we also investigated the effects of the presence of ovarian follicles, aquaporin water permeability (hgcl(2)), and yolk proteolysis (bafilomycin a1) inhibitors, gap junction uncouplers (carbenoxolone and 1-octanol), a 3beta-hydroxysteroid dehydrogenase inhibitor (t ... | 2009 | 19623568 |
| zinc is an essential trace element for spermatogenesis. | zinc (zn) plays important roles in various biological activities but there is little available information regarding its functions in spermatogenesis. in our current study, we further examined the role of zn during spermatogenesis in the japanese eel (anguilla japonica). human cg (hcg) was injected into the animals to induce spermatogenesis, after which the concentration of zn in the testis increased in tandem with the progression of spermatogenesis. staining of testicular cells with a zn-specif ... | 2009 | 19541612 |
| [modality and structure of japanese eel (anguilla japonica) during spermiogenesis]. | the special process and special structure which bring organelle during the spermiogenesis of japanese eel, anguilla japonica were observed by scanning electron microscope and transmission electron microscope. the process which spermatoblast became sperm including four special stages, the early stage, the middle stage, the later stage and the spermic stage, then came into being a normal mature sperm. in the early stage, cell nucleus became long form gradually by the oval form. in one side of the ... | 2009 | 19537199 |
| extracellular calcium-sensing receptor distribution in osmoregulatory and endocrine tissues of the tilapia. | the extracellular calcium-sensing receptor (casr) serves an important detector function in vertebrate ca(2+) homeostasis. in this study, we surveyed using immunohistochemistry the tissue and cellular distribution of the casr protein in the mozambique tilapia (oreochromis mossambicus) and the japanese eel (anguilla japonica). specifically, we examined receptor expression in ion-transporting barrier tissues that may be directly responsive to extracellular ca(2+) levels, and in tissues that are imp ... | 2009 | 19523399 |
| effect of eel galectin ajl-1 on periodontopathic bacterial biofilm formation and their lipopolysaccharide-mediated inflammatory cytokine induction. | porphyromonas gingivalis, prevotella intermedia and aggregatibacter actinomycetemcomitans, infectious pathogenic bacteria found in oral biofilm, cause periodontal disease. the inhibitory effect of ajl-1, a galectin present in the skin mucus of the japanese eel anguilla japonica, on biofilm formation by each of these strains was investigated by staining adherent bacteria on culture plates with crystal violet. an atp bioluminescence assay was used to determine whether inhibition of biofilm formati ... | 2009 | 19505801 |
| arsenic inhibits in vitro spermatogenesis and induces germ cell apoptosis in japanese eel (anguilla japonica). | the precise mechanism and direct effects of arsenic on fish, particularly in reproduction, are not well clarified. the aim of this study is to investigate the direct influence of arsenic on fish spermatogenesis using the japanese eel (anguilla japonica) in vitro testicular organ culture system. eel testicular fragments were cultured in vitro with 0.1-100 microm arsenic with or without human chorionic gonadotropin (hcg) for 6 or 15 days at 20 degrees c. arsenic treatment provoked a dose-dependent ... | 2009 | 19494047 |
| degradation of oxolinic acid and flumequine in aquaculture pond waters and sediments. | oxolinic acid (oa) and flumequine (flu) are two of the quinolone antibiotics (qas) that are widely used in aquaculture. the purpose of this study was to understand the fates of oa and flu in waters and sediment slurries from aquaculture ponds in a laboratory experiment. waters and sediments were sampled from an eel (anguilla japonica) pond and a shrimp (penaeus vannamei) pond. the effects of light, microbial activities, and temperature on the degradation of these two qas were elucidated. results ... | 2009 | 19230954 |
| rapid determination of arsenic species in freshwater organisms from the arsenic-rich hayakawa river in japan using hplc-icp-ms. | speciation analyses of water-soluble arsenicals from freshwater and biological samples collected from the hayakawa river (kanagawa, japan), which contains a high concentration of arsenic, were performed using high performance liquid chromatography/inductively coupled plasma mass spectrometry (hplc-icp-ms). river water contained only arsenate, which is a pentavalent inorganic arsenical. the water bug stenopsyche marmorata contained inorganic arsenicals accounting for 77% of the water-soluble arse ... | 2009 | 19203781 |
| purification and characterization of a proteinaceous toxin from the serum of japanese eel anguilla japonica. | although eels are well known to contain toxins in the serum, their chemical properties have remained to be clarified for a long time. in this study, a proteinaceous toxin was purified from the serum of japanese eel anguilla japonica by anion-exchange hplc, hydroxyapatite hplc and gel filtration hplc. the toxin was lethal to both mice and crabs; the ld(50) of the purified toxin against mice (intravenous injection) and crabs (injection into body cavity) were estimated to be 670 and 450 mug kg(-1), ... | 2008 | 19015964 |
| population structure of the parasitic nematode anguillicola crassus, an invader of declining north atlantic eel stocks. | probably half of all animal species exhibit a parasitic lifestyle and numerous parasites have recently expanded their distribution and host ranges due to anthropogenic activities. here, we report on the population genetic structure of the invasive nematode anguillicola crassus, a parasite in freshwater eels, which recently spread from asia to europe and north america. samples were collected from the newly colonized naive host species anguilla anguilla (europe) and anguilla rostrata (north americ ... | 2008 | 18727770 |
| rectal water absorption in seawater-adapted japanese eel anguilla japonica. | marine teleosts drink large amounts of seawater to compensate for continuous osmotic water loss. we investigated a possible significant role of the rectum in water absorption in seawater-adapted eel. in rectal sacs filled with balanced salt solution (bss) and incubated in isotonic bss, water absorption was greater in seawater-adapted eel than in freshwater eel. since rectal fluid osmolality was slightly lower than plasma osmolality in seawater-adapted eel, effects of rectal fluid osmolality on w ... | 2008 | 18687408 |
| antidiuretic effect of eel anp infused at physiological doses in conscious, seawater-adapted eels, anguilla japonica. | atrial natriuretic peptide (anp) is known as a potent natriuretic/diuretic hormone in vertebrates. however, eel anp infused at doses that did not alter arterial blood pressure (0.3-3.0 pmol/kg/min) decreased urine volume and increased urinary na concentration in seawater (sw)-adapted eels but not in freshwater (fw)-adapted eels. the renal effects were dose-dependent and disappeared after infusate was switched back to a vehicle (0.9% nacl). urinary na excretion (volume x na concentration) did not ... | 1998 | 18466004 |
| effects of pituitary glycoprotein hormones and thyroid hormones on in-vitro vitellogenin incorporation into organ-cultured oocytes in the japanese eel, anguilla japonica. | the objectives of the present study were to establish a long-term culture system for previtellogenic ovarian fragments of the japanese eel (anguilla japonica) and to identify the effects of salmon pituitary glycoprotein fraction (spg), thyroxine (t4), and 3,5,3'-triiodothyronine (t3) on the uptake of vitellogenin (vtg) by cultured ovarian fragments evidenced by the appearance of yolk globules (ygs) within the oocytes. yolk globules first appeared in the oocytes incubated in media containing only ... | 2008 | 18393571 |
| vaccination of eels (anguilla japonica and anguilla anguilla) against anguillicola crassus with irradiated l3. | the original host of the swimbladder nematode anguillicola crassus, the japanese eel (anguilla japonica) and the recently colonized european eel (anguilla anguilla) were immunized with 40 irradiated (500 gy) 3rd-stage larvae (l3) of this parasite and challenged with an infection of 40 normal l3. the immunization induced a significant reduction of the number of adult worms developing from the challenge infection in a. japonica, but not in a. anguilla. the induced resistance (calculated using the ... | 2008 | 18302804 |
| parasite communities in eels of the island of reunion (indian ocean): a lesson in parasite introduction. | eel populations from the small rivers on the island of reunion (french overseas department in the indian ocean) were investigated with respect to the occurrence and abundance of helminths during the autumn of 2005. the native species anguilla marmorata (n = 80), anguilla bicolor (n = 23), and anguilla mossambica (n = 15) were studied. six species of helminths were identified, four of them having a definitely nonnative status. furthermore, unidentified intra-intestinal juvenile cestodes and extra ... | 2008 | 18288491 |
| ecological aspects of the japanese eel, anguilla japonica, collected from coastal areas of japan. | the ecological characteristics of 597 yellow and silver-stage japanese eels, anguilla japonica, were examined and compared among collection sites located at three different latitudes of japan (amakusa islands, mikawa bay, and sanriku coast) to provide basic data on this unusual catadromous fish species. eels were sexed and their total length, body weight, age, and growth rate based on otolith analysis was compared among sexes, stages, and collection sites. the overall sex ratio favored females ( ... | 2007 | 18271637 |
| bioconversion by functional p450 1a9 and p450 1c1 of anguilla japonica. | we indicated that two p450s (1a9 and 1c1) from japanese eel (anguilla japonica) metabolized 7-ethoxycoumarin, 7-ethoxyresorufin, and flavanone. at first, we constructed expression vectors for two types of p450 (1a9 and 1c1). the reduced co-difference spectra of escherichia coli cells transformed with these plasmids showed soret peaks (450 nm) that were typical of p450s. we performed bioconversion experiments in which substrates were added directly to incubation medium. the resulting metabolite(s ... | 2008 | 18243059 |
| the effects of five different glycans on innate immune responses by phagocytes of hybrid tilapia and japanese eels anguilla japonica. | the aim of this study was to evaluate the immune responses in hybrid tilapia (nile tilapia oreochromis niloticus x mozambique tilapia o. mossambicus) and japanese eels anguilla japonica after treatment with five glycans: barley, krestin, macrogard, scleroglucan, and zymosan. the effects of the glycans on the innate immune responses of the fish were investigated using the phagocytic index (pi), lysozyme activity, complement opsonization, and activation assay. the results of the lysozyme assay dem ... | 2007 | 18236632 |
| axoneme-dependent tubulin modifications in singlet microtubules of the drosophila sperm tail. | drosophila melanogaster sperm tubulins are posttranslationally glutamylated and glycylated. we show here that axonemes are the substrate for these tubulin c-terminal modifications. axoneme architecture is required, but full length, motile axonemes are not necessary. tubulin glutamylation occurs during or shortly after assembly into the axoneme; only glutamylated tubulins are glycylated. tubulins in other testis microtubules are not modified. only a small subset of total drosophila sperm axoneme ... | 2008 | 18205200 |
| a lectin (cflec-2) aggregating staphylococcus haemolyticus from scallop chlamys farreri. | lectins are a family of carbohydrate-recognition proteins which play crucial roles in innate immunity. in this study, a new lectin (cflec-2) gene was cloned from chlamys farreri by est and race approaches. the full-length cdna of cflec-2 was composed of 708bp, encoding a typical long form carbohydrate-recognition domain of 130 residues. the deduced amino acid sequence showed high similarity to brevican in homo sapiens, c-type lectin-1 and lectin-2 in anguilla japonica. the cdna fragment encoding ... | 2008 | 18203621 |
| dietary korean mistletoe enhances cellular non-specific immune responses and survival of japanese eel (anguilla japonica). | the present study was performed to investigate the immunostimulatory effects of korean mistletoe extract (km-110; viscum album coloratum) on the non-specific immune response and protection against aeromonas hydrophila infection in japanese eel (anguilla japonica). eels were fed under 4 regimes, 0%, 0.1%, 0.5% and 1.0% km-110 mixed diet. on day 14 after feeding, 15 fish from each group were injected i.p. with live a. hydrophila (3 x 10(6)cfu) and the remaining unchallenged fish from each group we ... | 2008 | 18023593 |
| identification of two insulin-like growth factor iis in the japanese eel, anguilla japonica: cloning, tissue distribution, and expression after growth hormone treatment and seawater acclimation. | to better understand the role of igfs in japanese eel, anguilla japonica, we cloned insulin-like growth factor-ii (igf-ii) cdnas and examined their mrna expression in several tissues. two eel igf-ii cdnas, eigf-ii-1 and eigf-ii-2, were cloned from the liver. a signal peptide and a mature peptide of both preproigf-iis were composed of 47 amino acids (aa) and 69 aa, but they differed at 17 aa and 13 aa, respectively. the e domain of eigf-ii-1 was 49 aa longer than that of eigf-ii-2, and differed a ... | 2008 | 17855139 |
| follicle-stimulating hormone induces spermatogenesis mediated by androgen production in japanese eel, anguilla japonica. | follicle-stimulating hormone (fsh) plays important roles in spermatogenesis. however, the biologic activity of fsh can vary in different vertebrate classes, and the definitive function of fsh has not been established. in this study, we investigated the functions of fsh on spermatogenesis using an in vitro culture system for japanese eel testis. the eel fsh receptor was expressed in testis tissue during the whole process of spermatogenesis, mainly by leydig cells that produce steroid hormones and ... | 2007 | 17761645 |
| base changes in the flic gene of edwardsiella tarda: possible effects on flagellation and motility. | edwardsiella tarda is a broad host-range pathogen infecting both animals and humans. e. tarda isolates from red sea bream pagrus major are non-motile, whereas isolates from japanese eel anguilla japonica and japanese flounder paralichthys olivaceus are motile with peritrichous flagella. we compared the flic gene coding for flagellin (flic) in motile and non-motile e. tarda strains isolated from diseased fish. twenty-two amino acid residues differed in the predicted flic amino acid sequences betw ... | 2007 | 17760384 |
| differential expression and regulation of gonadotropins and their receptors in the japanese eel, anguilla japonica. | eel species have a striking life cycle with a blockade of puberty until the oceanic migration. we report the first molecular data on eel gonadotropin receptors. the partial sequences cloned covered two-third of the open reading frame and included most of the extracellular and transmembrane domains. phylogenetic analysis partitioned the two eel gonadotropin receptors into the two teleost fshr and lhr clusters, respectively. real-time quantitative rt-pcr was used to quantify the expression of eel ... | 2007 | 17597622 |
| effect of osmotic shrinkage and hormones on the expression of na+/h+ exchanger-1, na+/k+/2cl- cotransporter and na+/k+ -atpase in gill pavement cells of freshwater adapted japanese eel, anguilla japonica. | it is well-known that gill epithelial cells are important in fish osmoregulation. however, studies on the effect of osmotic stress on the direct cellular responses of the gill epithelial cells are limited. in this paper, we aimed to determine the effects of osmotic hypertonicity, hormones and cellular signaling molecules on the expression of ion transporters in the cultured primary freshwater pavement cells (pvcs), prepared from freshwater-adapted eels (anguilla japonica). our data demonstrated ... | 2007 | 17562884 |
| heterosporis anguillarum infections in farm cultured eels (anguilla japonica) in korea. | ten eels (anguilla japonica) from a fish farm in korea were examined and diagnosed with a heterosporis infection. the gross lesions on the trunk were uneven and the concave parts were pasty. histopathologically, lyses of the trunk muscles, degenerative muscle fibers and the scattered spores were observed. the sporophorocyst (spc) contained several spores with a variety of shapes. some spc were disrupted and the spores in the spc were scattered in the muscle tissues. macrophages existed near the ... | 2007 | 17519567 |
| effects of lead, molybdenum, rubidium, arsenic and organochlorines on spermatogenesis in fish: monitoring at mekong delta area and in vitro experiment. | to estimate the influence of water contaminants on fish reproduction in the mekong delta area, we sampled cultivated male catfish (pangasianodon hypophthalmus), investigated testicular development, and measured persistent organic pollutants (pops) and trace element levels in muscle and liver, respectively. various testes sizes were observed although sampling took place during a short period. histological analysis revealed that all developmental stages of germ cells were observed in catfish with ... | 2007 | 17448548 |
| structural composition and anticoagulant activity of dermatan sulfate from the skin of the electric eel, electrophorus electricus (l.). | we determined the disaccharide composition of dermatan sulfate (ds) purified from the skin of the electric eel electrophorus electricus. ds obtained from the electric eel was composed of non-sulfated, mono-sulfated disaccharides bearing esterified sulfate groups at positions c-4 or c-6 of n-acetyl galactosamine (galnac), and disulfated disaccharides bearing esterified sulfate groups at positions c-2 of the uronic acid and at position c-4 or c-6 of galnac. the anticoagulant, antithrombotic and bl ... | 2007 | 17360213 |
| purification and characterization of a novel imidazole dipeptide synthase from the muscle of the japanese eel anguilla japonica. | we have purified a novel enzyme from eel white muscle which catalyzes the syntheses of imidazole dipeptides, such as carnosine (beta-alanyl-l-histidine), anserine (beta-alanyl-pi-methyl-l-histidine), and balenine (ophidine; beta-alanyl-tau-methyl-l-histidine), directly from their precursors. the enzyme was purified 1130-fold from eel muscle by a series of column chromatographies. although eel muscle contains a large amount of carnosine and only trace amounts of anserine and balenine, the anserin ... | 2007 | 17261377 |
| thyroid hormone receptor beta is widely expressed in the brain and pituitary of the japanese eel, anguilla japonica. | in a previous study, we cloned the thyroid hormone receptor betab (trbetab) from a teleostean fish, the japanese conger eel (conger myriaster). the gene encoding this receptor is expressed in the brain and pituitary. in this study, we cloned trbetab from the brain of the japanese eel (anguilla japonica) to extend our studies into thyroid hormone function in fish. rt-pcr analysis demonstrated that the trbetab transcripts were abundant in both the brain and pituitary. the trbetab cdna encoded a 37 ... | 2007 | 17150218 |
| occurrence of anguillicola crassus (nematoda: dracunculoidea) in japanese eels anguilla japonica from a river and an aquaculture unit in sw taiwan. | the infection by swimbladder nematodes of the genus anguillicola (dracunculoidea: anguillicolidae) was examined in 2 populations of the japanese eel anguilla japonica in sw taiwan. wild eels from the kao-ping river were compared with cultured eels from an adjacent aquaculture unit. only the cosmopolitan species anguillicola crassus was present. among wild eels, prevalence of infection varied between 21 and 62%, and mean intensity between 1.7 and 2.7 for adult worms. similar intensity values (1.3 ... | 2006 | 16956057 |
| roles of 11beta-hydroxysteroid dehydrogenase in fish spermatogenesis. | in fish spermatogenesis, the main action of progestins is generally regarded as the induction of sperm maturation. our previous in vitro study demonstrated that a progestin, 17alpha,20beta-dihydroxy-4-pregnen-3-one (dhp), induced the initiation of meiosis in spermatogenesis in the japanese eel (anguilla japonica). in the present study, to elucidate the molecular mechanisms underlying the action of dhp, we attempted to clone cdnas encoding genes whose expression was induced by dhp in eel testis, ... | 2006 | 16887910 |
| structural and expression analysis of hepatic vitellogenin gene during ovarian maturation in anguilla japonica. | vitellogenin (vtg), the precursor molecule for yolk, is synthesized in the liver under estrogenic control. in all oviparous species, including fish, the process of vitellogenesis is crucial to subsequent embryonic development. this study attempted to obtain the cdna encoding for vtg from female japanese eels, anguilla japonica. rapid amplification of cdna ends (race) and polymerase chain reaction (pcr) were used to amplify vtg cdna prepared from liver extracts. obtained pcr products were subclon ... | 2006 | 16854581 |
| expression of growth hormone family and growth hormone receptor during early development in the japanese eel (anguilla japonica). | in a previous study, we identified cdnas encoding the growth hormone receptor (eghr1) and eghr1 homologue (eghr2) in japanese eel (anguilla japonica). in the present study, changes in the developmental expression of growth hormone (gh), eghr1 and eghr2 were investigated in the japanese eel eggs and preleptocephali by rt-pcr and immunohistochemical methods in an attempt to examine the involvement of these proteins in larval growth. the gh transcripts and the production of gh protein were not dete ... | 2006 | 16828323 |
| transactivation activity of thyroid hormone receptors in fish (conger myriaster) in response to thyroid hormones. | we previously isolated cdnas encoding conger eel (conger myriaster) thyroid hormone (th) receptors (trs). in the present study, we investigated the transactivation activities of conger eel trs treated with ths (3,3',5-triiodo-l-thyronine [t3], l-thyroxine [t4], and 3,3',5'-triiodo-l-thyronine [rt3]), or ligands and activators of other nuclear receptors. following transient transfection into the japanese eel (anguilla japonica) hepatocyte cell line hepa-e1, the conger eel trs (ctrs) showed th-dep ... | 2006 | 16820313 |
| an endothelial-cell-enriched primary culture system to study vascular endothelial growth factor (vegf a) expression in a teleost, the japanese eel (anguilla japonica). | a partial gene for eel (anguilla japonica) vascular endothelial growth factor (vegf) has been cloned and an endothelial-cell-enriched primary culture derived from rete mirabile established to study regulation of the expression of the eel vegf gene. cells were cultured in m199 medium containing 0.1% fetal calf serum (fcs) and serum-free m199 medium for long-and short-term experiments, respectively. cells were separately treated with cobalt ions (co2+), basic fibroblast growth factor (bfgf), and e ... | 2006 | 16807025 |
| the swimbladder nematode anguillicola crassus in the european eel anguilla anguilla and the japanese eel anguilla japonica: differences in susceptibility and immunity between a recently colonized host and the original host. | the swimbladder nematode anguillicola crassus originates from asia where it is a parasite of the japanese eel anguilla japonica. after its introduction to europe about 25 years ago, the parasite spread rapidly within the indigenous populations of the european eel anguilla anguilla and subsequently the prevalence and mean intensity appeared to stabilize. under experimental and aquaculture conditions the naïve new host appears to be more susceptible to a. crassus compared to the original host. bot ... | 2006 | 16768856 |
| hosts and parasites as aliens. | over the past decades, various free-living animals (hosts) and their parasites have invaded recipient areas in which they had not previously occurred, thus gaining the status of aliens or exotics. in general this happened to a low extent for hundreds of years. with variable frequency, invasions have been followed by the dispersal and establishment of non-indigenous species, whether host or parasite. in the literature thus far, colonizations by both hosts and parasites have not been treated and r ... | 2006 | 16768855 |
| identification of eel ghrelin in plasma and stomach by radioimmunoassay and histochemistry. | the structure of ghrelin has been determined in the japanese eel, anguilla japonica. in this study, we identified immunoreactive ghrelin in extracts from plasma and stomach of the eel by radioimmunoassay (ria) using an antiserum against octanoylated rat ghrelin [1-11]. using the antiserum, we examined localization of ghrelin-immunopositive cells in the eel stomach. detection of ghrelin mrna-expressing cells was also attempted in the eel stomach using a crna probe specific for the eel ghrelin gen ... | 2006 | 16762351 |
| cloning and characterization of a cdna encoding cholesterol side-chain cleavage cytochrome p450 (cyp11a1): tissue-distribution and changes in the transcript abundance in ovarian tissue of japanese eel, anguilla japonica, during artificially induced sexual development. | cholesterol side-chain cleavage cytochrome p450 (cyp11a1: p450scc) is a crucial steroidogenic enzyme that catalyzes an initial step in the production of all classes of steroids. a cdna encoding japanese eel p450scc was cloned and characterized. the cdna putatively encoded 521 amino acid residues with high homology to those of other vertebrate forms. the recombinant p450scc produced in cos-7 cells efficiently catalyzed the conversion of 25-hydroxycholesterol into pregnenolone. by northern blot, a ... | 2006 | 16616842 |
| [the study of ribosomal protein l15 cdna sequences as a molecular marker in the teleostei phylogenetic analysis]. | in this study, the full-length cdnas of rpl15 (ribosomal protein l15) gene were cloned and sequenced from fifteen fishes of five orders under teleostei. the complete orf sequences were analyzed for phylogenetic reconstruction for the first time to evaluate the potential of rpl15 gene as a novel marker in resolving teleostean phylogenetic relationships. the resultant nj, mp and ml trees with anguilla japonica as the outgroup were generally resolved and largely congruent with the morphology-based ... | 2006 | 16520312 |
| [induction of pond-cultivated anguilla japonica gonadial development by controlling temperature in high temperature season]. | in this study, the pond-cultivated anguilla japonica was induced to mature by artificially controlling temperature at 18 +/- 2 degrees c in high temperature season (from june to september), with injection of hcg and cpe. the results showed that both the male and the female eel could be induced to mature, and the artificial maturation induction rate was 73.33% and 67.77%, while under non-controlling temperature 25 approximately 32 degrees c, the maturation induction rate was 0 and 33.33%, respect ... | 2005 | 16471368 |
| cloning and characterization of vitellogenin cdna from the common japanese conger (conger myriaster) and vitellogenin gene expression during ovarian development. | the major yolk protein precursor, vitellogenin (vtg) was detected in plasma from vitellogenic females and estradiol-17beta (e2)-treated immature females, but not in males and immature females by western blotting in common japanese conger conger myriaster. its molecular mass was approximately 180 kda under denaturing and reducing conditions. the common japanese conger vtg cdna was cloned from the liver of vitellogenic female. it contains 5110 nucleotides including an open reading frame that encod ... | 2006 | 16460978 |
| molecular cloning and characterization of growth hormone receptor and its homologue in the japanese eel (anguilla japonica). | two cdnas encoding growth hormone receptor (ghr)-like genes, eghr1 and eghr2, were isolated from japanese eel (anguilla japonica) liver tissue. the putative eel ghr proteins showed conserved structural features of vertebrate ghrs, including six cysteine residues and a ygefs motif in the extracellular domain, a single transmembrane region, and proline-rich box 1 and box 2 domains. northern blot analysis showed a single eghr1 transcript in liver, while two sizes of eghr2 transcripts, thought to be ... | 2006 | 16458558 |
| molecular cloning and gene expression of spo11 during spermatogenesis in the japanese eel, anguilla japonica. | spo11 is a protein involved specifically in the meiotic recombination in several species, however, it is little characterized in lower vertebrates. we identified a cdna encoding spo11 from the testis of japanese eel, anguilla japonica. the deduced amino acid sequence of eel spo11 was more than 60% identical with human and mouse spo11s. in order to examine changes in the expression and localization of spo11 during spermatogenesis induced by the injection of human chorionic gonadotropin (hcg) and ... | 2006 | 16426883 |
| distribution of polychlorinated biphenyls in the water, sediment and fish from the pearl river estuary, china. | the concentrations and distribution of polychlorinated biphenyls were determined in water, surface sediments and fishes from the estuaries of pearl river, pr china. the results showed that the concentrations ranged from 2.47 ng l-1 to 6.75 ng l-1 in water, from 11.13 ng g-1 to 23.23 ng g-1 in sediments (dry weight) and from 68.64 ng g-1 to 316.85 ng-1 in fish (lipid weight). it was found that the feeding habits of fish were very significant in the accumulation of polychlorinated biphenyls and pa ... | 2005 | 16374930 |
| apolipoprotein complexity in japanese eel anguilla japonica: truncated apolipoprotein a-i and apolipoprotein a-i-like protein in plasma lipoproteins. | a truncated apolipoprotein (apo) a-i with a molecular weight (m(r)) of 26 kda was first isolated from the plasma high density lipoproteins of an atypical japanese eel (anguilla japonica). interestingly, this eel contained a very small amount of intact apoa-i (m(r)28 kda) in the plasma, although serine protease inhibitors were present throughout the plasma preparation. the n-terminal sequence of 20 amino acids in truncated apoa-i was completely identical with that of intact apoa-i. another apolip ... | 2005 | 16306716 |
| steroidogenic activities of follicle-stimulating hormone in the ovary of japanese eel, anguilla japonica. | to clarify the physiological functions of follicle-stimulating hormone (fsh) during oogenesis in japanese eel, anguilla japonica, the steroidogenic activities of recombinant japanese eel fsh (rjefsh) were assessed in the eel ovary. female eel were injected with salmon pituitary homogenate to enhance the ovarian development, and the ovaries at different developmental stages were subjected to steroidogenic bioassay. these ovaries could be classified into three types according to oocyte growth and ... | 2006 | 16297918 |
| first record of ostracods as natural intermediate hosts of anguillicola crassus, a pathogenic swimbladder parasite of eels anguilla spp. | the ostracod physocypria nipponica (ostracoda: candonidae) was found (prevalence 14.2%) to be the only intermediate host of the nematode anguillicola crassus (nematoda: anguillicolidae), a pathogenic swimbladder parasite of eels, in a greenhouse-heated culture pond at isshiki, aichi prefecture, japan. japanese eels anguilla japonica from the same pond were found to be infected by adult a. crassus (prevalence 71.8%, intensity 1 to 6). this indicates that a. crassus could complete its life cycle u ... | 2005 | 16231644 |
| differential expression of neural and gonadal aromatase enzymatic activities in relation to gonadal development in japanese eel, anguilla japonica. | the objectives of the present study were to investigate the enzymatic characteristics of brain aromatase in japanese eel, anguilla japonica, as well as the correlations between aromatase activities in various tissues (brain regions, pituitary, and gonads) and ovarian development. eel brain aromatase exhibited a k(m) of 75 nm and a v(max) of 1.14 fmol/min mg protein (91.5 fmol/h mg protein), indicating an enzymatic activity much lower than in other teleosts but similar to that in mammals. this su ... | 2005 | 16106412 |
| purification of follicle-stimulating hormone from immature japanese eel, anguilla japonica, and its biochemical properties and steroidogenic activities. | follicle-stimulating hormone (fsh) was purified, for the first time, from immature japanese eel, anguilla japonica, and its biochemical properties were investigated. fsh was extracted from immature eel pituitaries and purified by gel-filtration on sephadex g-100, and two step anion-exchange chromatography: stepwise elution on de-52, followed by gradient elution on tsk-gel super-q using hplc. purification was performed using its molecular mass and the positive reaction with anti-japanese eel (je) ... | 2005 | 15894317 |
| androgen action on hepatic vitellogenin synthesis in the eel, anguilla japonica is suppressed by an androgen receptor antagonist. | involvement of additional hormones other than estrogen in the control of vitellogenin (vg) synthesis has been suggested in fish. however, no satisfactory explanation on the mechanism of the action of these hormones has been reported. in this study, we have exploited the possibility of androgen receptor mediation during the androgen action on the pathway of vg synthesis. hepatocytes were prepared from sexually immature japanese eel anguilla japonica and treated with estradiol-17beta (e2), 17alpha ... | 2005 | 15878656 |
| "blood-contacting neurons" in the brain of the japanese eel anguilla japonica. | to discriminate "blood-contacting neurons" within the brain of the eel, evans blue (eb) was injected intraperitoneally. after five days, six brain areas were externally stained blue with the dye; the saccus dorsalis (sd), the epiphysis (e), the area postrema (ap), the posterior part of the magnocellular preoptic nucleus (pm), the pituitary (pit), and the saccus vasculosus (sv). among the eb-positive area, some cells in the pm, the anterior tuberal nucleus (nat) and the ap were discriminated as t ... | 2005 | 15828012 |
| a novel cysteine protease inhibitor with lectin activity from the epidermis of the japanese eel anguilla japonica. | a novel cysteine protease inhibitor (eel-cpi-1) was isolated from the epidermis of the eel. eel-cpi-1 was shown to bind strongly to both lactose- and carboxymethylated papain-affinity gels. its molecular mass under reducing condition was determined to be 18 kda by sds-polyacrylamide gel electrophoresis but approximately 30.5 kda under non-reducing-conditions. eel-cpi-1 inhibited papain (k(i)=18 nm) and ficin (k(i)=120 nm) competitively. combined with the data on amino acid and sequence analysis, ... | 2005 | 15820140 |
| roles of slc13a1 and slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater. | sulfate is required for proper cell growth and development of all organisms. we have shown that the renal sulfate transport system has dual roles in euryhaline eel, namely, maintenance of sulfate homeostasis and osmoregulation of body fluids. to clarify the physiological roles of sulfate transporters in teleost fish, we cloned orthologs of the mammalian renal sulfate transporters slc13a1 (nasi-1) and slc26a1 (sat-1) from eel (anguilla japonica) and assessed their functional characteristics, tiss ... | 2005 | 15802556 |
| natriuretic peptide guanylyl cyclase receptors in the kidney of the japanese eel, anguilla japonica. | natriuretic peptides are linked to osmoregulation, cardiovascular and volume regulation in fishes. the peptides bind to two guanylyl-cyclase-linked receptors, natriuretic peptide receptor-a (npr-a) and npr-b, to elicit their effects. atrial natriuretic peptide (anp) binds principally to npr-a, whereas c-type natriuretic peptide (cnp) binds to npr-b. the teleost kidney has an important role in the maintenance of fluid and electrolyte balance; therefore, the location of npr-a and npr-b in the kidn ... | 2005 | 15789218 |