Publications

molecular genetic characterization of a locus that contains duplicate adh genes in drosophila mojavensis and related species.	drosophila mojavensis and other species of the mulleri subgroup contain a duplicate gene encoding the enzyme alcohol dehydrogenase (adh). studies on the genetic relationship of the two genes using electrophoretic variants show them to be closely linked. we have cloned a 13.5-kb fragment of d. mojavensis dna into the lambda vector, charon 30. this fragment contains both adh genes separated by approximately 2 kb of dna. the clone hybridized to a single position on chromosome 3 in d. mojavensis fol ...	1986	3000866
microbial colonization of injured cactus tissue (stenocereus gummosus) and its relationship to the ecology of cactophilic drosophila mojavensis.	necrotic tissue of agria cactus (stenocereus gummosus) serves as a feeding and breeding substrate for drosophila mojavensis. this fly species is one of the four endemic drosophila species in the sonoran desert. freeze injuries were created in arms of agria cactus in mexico to study the events of microbial colonization. facultative anaerobic bacteria were the first microbes to be detected, and the exclusion of large arthropods by covering the injuries with netting did not affect bacterial coloniz ...	1989	2705763
courtship behavior and control of reproductive isolation in drosophila mojavensis: genetic analysis of population hybrids.	drosophila mojavensis from the sonora region and baja california show asymmetrical sexual isolation in the laboratory: males from sonora mate equally frequently with sonora and baja females, while the mating success of baja males with sonora females is reduced. this failure has been localized to three separate behavioral landmarks occurring during courtship. genetic analysis was conducted using reciprocal f1 hybrids of sonora and baja strains to examine inheritance patterns of the responsible co ...	1990	2256893
serine esterases: structural conservation during animal evolution and variability in enzymatic properties in the genus drosophila.	both general esterases and acetylcholinesterases have been shown to be members of a homologous superfamily of serine esterases. a comparison of n-terminal sequences demonstrates that esterase-4 and -5 from drosophila mojavensis belong to this family as well, with esterase-6 and esterase-p from d. melanogaster being the closest relatives. in order to investigate the presence of immunologically related esterases in other drosophila species, crude larval extracts from five species were applied to t ...	1990	2124571
characterization of the structure and evolution of the adh region of drosophila hydei.	drosophila of the repleta group have a duplication of the gene which encodes alcohol dehydrogenase (adh). we report the nucleotide sequence of an 8.4-kb region of genomic dna of drosophila hydei which includes the entire adh region. analysis of this sequence reveals similarity in organization to the adh region of drosophila mojavensis and drosophila mulleri of the mulleri subgroup, with three genes ordered 5' to 3', adh-psi, adh-2, adh-1. deletion of a nucleotide in the second codon of each pseu ...	1991	2004708
remating effects on the genetic structure of female life histories in populations of drosophila mojavensis.	a quantitative genetic analysis of nine adult fitness components was performed in two populations of cactophilic drosophila mojavensis under natural conditions of fermenting cactus and ethanol vapour. female progeny from 18 sires and 36 dams were treated to a range of six exposure periods to males to assess effects of remating frequency on female fitness. lifetime fecundity increased with increasing male exposure, but longevity showed an intermediate optimum with temporary exposure to males of 2 ...	1992	1612926
involvement of cytochrome p450 in host-plant utilization by sonoran desert drosophila.	the four drosophila species endemic to the sonoran desert (drosophila mettleri, drosophila mojavensis, drosophila nigrospiracula, and drosophila pachea) utilize necrotic cactus tissue or soil soaked by rot exudate as breeding substrates. each drosophila species uses a different cactus species as its primary host. d. pachea is limited to senita cactus by a biochemical dependency on unusual sterols available only in that cactus. for the other drosophila species, no such chemical dependencies exist ...	1992	1465429
delineation of cis-acting sequences required for expression of drosophila mojavensis adh-1.	the control of expression of the adh-1 gene of drosophila mojavensis has been analyzed by transforming adh null drosophila melanogaster hosts with p element constructs which contain d. mojavensis adh-1 having deletions of different extent in the 5' and 3' ends. adh-1 expression in the d. melanogaster hosts is qualitatively similar to expression in d. mojavensis, although expression is quantitatively lower in transformants. deletions of the 5' end indicate that information required for normal tem ...	1992	1317314
linkage disequilibrium between functionally related enzyme loci of drosophila mojavensis.	gametic frequencies at four enzyme loci of the second chromosome of drosophila mojavensis race b were obtained from six localities. the four loci were selected so that they code for functionally related enzymes; two code for esterases, one for xanthine dehydrogenase and one for acetaldehyde oxidase. in drosophila the two latter enzymes are under the same regulatory control and their physiological functions might be related as well. electrophoretic variants at the locicoding for these two enzymes ...	1976	990995
patterns of molecular variation. ii. associations of electrophoretic mobility and larval substrate within species of the drosophila mulleri complex.	electromorphic variation among populations of drosophila mojavensis, d. arizonensis and d. longicornis was examined for seven genetic loci. the average electrophoretic mobility for a population was used as the metric. d. mojavensis and d. arizonensis use larval substrates in different parts of their geographic ranges, while d. longicornis is more narrowly restricted to different species of the cactus opuntia in different localities. there is marked electromorphic variation among populations of e ...	1977	838268
structural flexibility of isozyme variants: genetic variants in drosophila disguised by cofactor and subunit binding.	wild populations of drosophila mojavensis exhibit considerable conformational variation in the nad+-free form of alcohol dehydrogenase (alcohol:nad+ oxidoreductase; ec 1.1.1.1). the variation appears genetic, as it does not occur within an inbred strain. the nad+-bound form of alcohol dehydrogenase, present in the same individuals, does not exhibit the variation, suggesting that the binding of nad+ acts to stabilize conformation. such cofactor binding to enzymes may thus conceal considerable var ...	1978	203939
extension of longevity in drosophila mojavensis by environmental ethanol: differences between subraces.	drosophila mojavensis adults, which breed and feed on necrotic cacti, show an increase in longevity when exposed to atmospheric ethanol. the increase in longevity is accompanied by retention of mature ovarioles and is independent of diet. differences in longevity among strains from different localities were detected for females. strains from arizona and sonora, mexico, showed the greatest increase in longevity, while strains from baja california, mexico, showed the least increase. these differen ...	1977	13369