Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| tribendimidine: mode of action and nachr subtype selectivity in ascaris and oesophagostomum. | the cholinergic class of anthelmintic drugs is used for the control of parasitic nematodes. one of this class of drugs, tribendimidine (a symmetrical diamidine derivative, of amidantel), was developed in china for use in humans in the mid-1980s. it has a broader-spectrum anthelmintic action against soil-transmitted helminthiasis than other cholinergic anthelmintics, and is effective against hookworm, pinworms, roundworms, and strongyloides and flatworm of humans. although molecular studies on c. ... | 2015 | 25679515 |
| a globin domain in a neuronal transmembrane receptor of caenorhabditis elegans and ascaris suum: molecular modeling and functional properties. | we report the structural and biochemical characterization of glb-33, a putative neuropeptide receptor that is exclusively expressed in the nervous system of the nematode caenorhabditis elegans. this unique chimeric protein is composed of a 7-transmembrane domain (7tm), glb-33 7tm, typical of a g-protein-coupled receptor, and of a globin domain (gd), glb-33 gd. comprehensive sequence similarity searches in the genome of the parasitic nematode, ascaris suum, revealed a chimeric protein that is sim ... | 2015 | 25666609 |
| seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the venezuelan delta region. | the present study aimed at measuring seropositivities for infection by ascaris suum and toxocara canis using the excretory/secretory (e/s) antigens from ascaris suum (aes) and toxocara canis (tes) within an indigenous population. in addition, quantification of cytokine expressions in peripheral blood cells was determined. a total of 50 warao indigenous were included; of which 43 were adults and seven children. in adults, 44.1% were seropositive for both parasites; whereas children had only serop ... | 2015 | 25651326 |
| detection of intestinal parasites on field-grown strawberries in the federal district of brazil. | this study evaluated the presence of pathogenic human parasites on field-grown strawberries in the federal district of brazil. | 2015 | 25626665 |
| cloning and characterization of a novel sigma-like glutathione s-transferase from the giant panda parasitic nematode, baylisascaris schroederi. | baylisascaris schroederi, an intestinal nematode of the giant panda, is the cause of the often fatal disease, baylisascariasis. glutathione s-transferases (gsts) are versatile enzymes that can affect parasite survival and parasite-host interactions and, are therefore, potential targets for the development of diagnostic tests and vaccines. | 2015 | 25613998 |
| functional and phylogenetic characterization of proteins detected in various nematode intestinal compartments. | the parasitic nematode intestine is responsible for nutrient digestion and absorption, and many other processes essential for reproduction and survival, making it a valuable target for anthelmintic drug treatment. however, nematodes display extreme biological diversity (including occupying distinct trophic habitats), resulting in limited knowledge of intestinal cell/protein functions of fundamental or adaptive significance. we developed a perfusion model for isolating intestinal proteins in asca ... | 2015 | 25609831 |
| the anti-inflammatory mediator palmitoylethanolamide enhances the levels of 2-arachidonoyl-glycerol and potentiates its actions at trpv1 cation channels. | palmitoylethanolamide (pea) is an endogenous congener of anandamide and potentiates its actions at cannabinoid cb1 and cb2 receptors, and at transient receptor potential vanilloid type-1 (trpv1) channels. the other endocannabinoid, 2-arachidonoylglycerol (2-ag), was recently suggested to act as a trpv1 channel agonist. we investigated if pea enhanced levels of 2-ag in vitro or in vivo and 2-ag activity at trpv1 channels. | 2016 | 25598150 |
| nadh→nad⁺ transhydrogenation in adult ascaris suum mitochondria. | although lacking an nadph→nad(+) transhydrogenase system, the essentially energetically anaerobic mitochondria of the adult intestinal nematode ascaris suum display an inner membrane-associated nadh→nad(+) transhydrogenation reaction. this reaction is considered to be reflective of a mechanism(s) that acts in catalyzing a transmembrane translocation of reducing equivalents from nadh in the intermembrane space to matrix nad(+), thereby forming matrix nadh that would serve in electron transport. a ... | 2015 | 25587625 |
| peptidases compartmentalized to the ascaris suum intestinal lumen and apical intestinal membrane. | the nematode intestine is a tissue of interest for developing new methods of therapy and control of parasitic nematodes. however, biological details of intestinal cell functions remain obscure, as do the proteins and molecular functions located on the apical intestinal membrane (aim), and within the intestinal lumen (il) of nematodes. accordingly, methods were developed to gain a comprehensive identification of peptidases that function in the intestinal tract of adult female ascaris suum. peptid ... | 2015 | 25569475 |
| characterization of the ca2+-gated and voltage-dependent k+-channel slo-1 of nematodes and its interaction with emodepside. | the cyclooctadepsipeptide emodepside and its parent compound pf1022a are broad-spectrum nematicidal drugs which are able to eliminate nematodes resistant to other anthelmintics. the mode of action of cyclooctadepsipeptides is only partially understood, but involves the latrophilin lat-1 receptor and the voltage- and calcium-activated potassium channel slo-1. genetic evidence suggests that emodepside exerts its anthelmintic activity predominantly through slo-1. indeed, slo-1 deficient caenorhabdi ... | 2014 | 25521608 |
| contribution of transcription to animal early development. | in mature gametes and during the oocyte-to-embryo transition, transcription is generally silenced and gene expression is post-transcriptionally regulated. however, we recently discovered that major transcription can occur immediately after fertilization, prior to pronuclear fusion, and in the first cell division of the oocyte-to-embryo transition in the nematode ascaris suum. we postulate that the balance between transcriptional and post-transcriptional regulation during the oocyte-to-embryo tra ... | 2014 | 25483604 |
| molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm ancylostoma ceylanicum. | nematodes are unable to synthesize fatty acids de novo and must acquire them from the environment or host. it is hypothesized that two unique classes of fatty acid and retinol binding proteins that nematodes produce (fatty acid and retinol binding (far) and nematode polyprotein antigen/allergen (npa)) are used to meet this need. a partial cdna has been cloned corresponding to four subunits of a putative ancylostoma ceylanicum npa (acenpa). the translated amino acid sequence of acenpa shares sequ ... | 2014 | 25481749 |
| comparison of methodologies for enumerating and detecting the viability of ascaris eggs in sewage sludge by standard incubation-microscopy, the baclight live/dead viability assay and other vital dyes. | the aim of this study was to evaluate the live/dead baclight viability kit as a method for enumerating viable eggs of ascaris suum in sewage sludge as a surrogate for the human roundworm. the number and viability status of eggs of a. suum were accurately measured directly in sewage sludge samples by the baclight method, compared to the conventional incubation-microscopy procedure. baclight stains were not toxic to a. suum eggs, in contrast to some conventional vital dyes which disrupted viable e ... | 2015 | 25462759 |
| diethylcarbamazine increases activation of voltage-activated potassium (slo-1) currents in ascaris suum and potentiates effects of emodepside. | diethylcarbamazine is a drug that is used for the treatment of filariasis in humans and animals; it also has effects on intestinal nematodes, but its mechanism of action remains unclear. emodepside is a resistance-busting anthelmintic approved for treating intestinal parasitic nematodes in animals. the novel mode of action and resistance-breaking properties of emodepside has led to its use against intestinal nematodes of animals, and as a candidate drug for treating filarial parasites. we have p ... | 2014 | 25411836 |
| the in vitro effect of ivermectin on the activity of trehalose synthesis pathway enzymes and their mrna expression in the muscle of adult female ascaris suum (nematoda). | the in vitro effect of ivermectin lethal dose on the activity of trehalose-6-phosphate synthase (tps) and phosphatase (tpp) and the expression of their mrna (tps1, tps2, and tpp genes) in the muscle of adult female ascaris suum was investigated. the presence of ivermectin in the medium caused a decrease in tps and tpp activities during the experiment compared with the start and control groups. the exception was the group of worms grown for 8 hours in a ivm solution, in which there was a little h ... | 2014 | 25405239 |
| immunoblot for the detection of ascaris suum-specific antibodies in patients with visceral larva migrans (vlm) syndrome. | visceral larva migrans (vlm) syndrome caused by toxocara canis larvae was first described in the 1950s. the role of other nematode larvae, i.e. the pig roundworm ascaris suum as a causative agent of visceral larva migrans-associated symptoms like general malaise, cough, liver dysfunction, hypereosinophilia with hepatomegaly and/or pneumonia, was discussed controversially during the last decades. recent serological screening studies for specific a. suum antibodies carried out in the netherlands a ... | 2015 | 25367210 |
| comparison of the effect of the chosen species of saprotrophic fungi on the development of toxocara canis and ascaris suum eggs. | the study aim was to compare the antagonistic interaction between saprotrophic soil fungi and embryonic development of geohelminths toxocara canis and ascaris suum. the experimental cultures were fertilized eggs of t.canis and a. suum incubated together with mycelium of strains: fusarium culmorum, metarhizium anisopliae,paecilomyces fumosoroseus, trichoderma viride and trichothecium roseum. in the control cultures the eggs of both nematode species were incubated without fungi. the experiment was ... | 2014 | 25281819 |
| anthelmintic efficacy of ivermectin and abamectin, administered orally for seven consecutive days (100 µg/kg/day), against nematodes in naturally infected pigs. | the present study aimed to evaluate ivermectin and abamectin, both administered orally in naturally infected domestic swine, as well as analysing if the epg (eggs per gram of faeces) values were equivalent with the ivermectin and abamectin efficacy obtained by parasitological necropsies. the animals were randomly selected based on the average of three consecutive epg counts of strongylida, ascaris suum and trichuris for experiment i, and of strongylida and trichuris for experiment ii. after the ... | 2014 | 25278142 |
| beta carbonic anhydrases: novel targets for pesticides and anti-parasitic agents in agriculture and livestock husbandry. | the genomes of many insect and parasite species contain beta carbonic anhydrase (β-ca) protein coding sequences. the lack of β-ca proteins in mammals makes them interesting target proteins for inhibition in treatment of some infectious diseases and pests. many insects and parasites represent important pests for agriculture and cause enormous economic damage worldwide. meanwhile, pollution of the environment by old pesticides, emergence of strains resistant to them, and their off-target effects a ... | 2014 | 25174433 |
| molecular cloning and analysis of ancylostoma ceylanicum glutamate-cysteine ligase. | glutamate-cysteine ligase (gcl) is a heterodimer enzyme composed of a catalytic subunit (gclc) and a modifier subunit (gclm). this enzyme catalyses the synthesis of γ-glutamylcysteine, a precursor of glutathione. cdnas of the putative glutamate-cysteine ligase catalytic (ace-gclc) and modifier subunits (ace-gclm) of ancylostoma ceylanicum were cloned using the race-pcr amplification method. the ace-gclc and ace-gclm cdnas encode proteins with 655 and 254 amino acids and calculated molecular mass ... | 2014 | 25092620 |
| antibacterial activity of recombinant pig intestinal parasite cecropin p4 peptide secreted from pichia pastoris. | cecropins (cec) are antibacterial peptides and their expression is induced in a pig intestinal parasite ascaris suum by bacterial infection. to explore the usefulness of its activity as an antibiotic, cecp4 cdna was prepared and cloned into the ppicz b expression vector and followed by the integration into aox1 locus in pichia pastoris. the supernatants from cell culture were collected after methanol induction and concentrated for the test of antimicrobial activity. the recombinant p. patoris ha ... | 2014 | 25049952 |
| role of posttranslational modifications in c. elegans and ascaris spermatogenesis and sperm function. | generally, spermatogenesis and sperm function involve widespread posttranslational modification of regulatory proteins in many different species. nematode spermatogenesis has been studied in detail, mostly by genetic/molecular genetic techniques in the free-living caenorhabditis elegans and by biochemistry/cell biology in the pig parasite ascaris suum. like other nematodes, both of these species produce sperm that use a form of amoeboid motility termed crawling, and many aspects of spermatogenes ... | 2014 | 25030766 |
| development and survival of ascaris suum eggs in deep litter of pigs. | summary indoor transmission of ascaris suum partly depends on the physico-chemical conditions in bedding material. temperature, ph, aqueous ammonia, moisture, occurrence and development of a. suum eggs were therefore compared in different areas (resting, intermediate and latrine) of two deep litter pens on an organic farm in four seasons. there was some variation, but mean ammonia levels were generally very low (1·0-2·6 mm) and ph levels were moderate (8·04-8·88) in all three areas. relatively, ... | 2014 | 25017881 |
| antiparasitic efficacy of a novel plant-based functional food using an ascaris suum model in pigs. | ascaris lumbricoides is the most prevalent soil-transmitted helminth (sth) infection of human beings worldwide. chemotherapy with synthetic anthelmintics such as albendazole, mebendazole, and pyrantel pamoate is the current method of treatment; however, the emergence of anthelmintic resistance could substantially decrease the efficacy of such treatments and the sustainability of sth control programs. additionally, benzimidazoles are not recommended for pregnant women or children under age one. a ... | 2014 | 24979686 |
| immunomodulation of liver injury by ascaris suum extract in an experimental model of autoimmune hepatitis. | adult worm extract from ascaris suum (asc) has immunosuppressive activity and elicits th2/il-4/il-10 response. this study evaluated the prophylactic and therapeutic effect of asc in a murine model of concanavalin a (cona)-induced autoimmune hepatitis (aih). balb/c mice received cona, iv, (20 mg/kg), and three groups of animals were formed: (1) aih, received only cona; (2) aih + asc prophylactic, treated with asc (1 mg/ml), ip, 30 min before of the aih; and (3) aih + asc therapeutic, treated with ... | 2014 | 24951170 |
| proteomic analysis of adult ascaris suum fluid compartments and secretory products. | strategies employed by parasites to establish infections are poorly understood. the host-parasite interface is maintained through a molecular dialog that, among other roles, protects parasites from host immune responses. parasite excretory/secretory products (esp) play major roles in this process. understanding the biology of protein secretion by parasites and their associated functional processes will enhance our understanding of the roles of esp in host-parasite interactions. | 2014 | 24901219 |
| survival of ascaris suum and ascaridia galli eggs in liquid manure at different ammonia concentrations and temperatures. | eggs of ascaris suum from pigs are highly resistant and commonly used as a conservative indicator of pathogen inactivation during slurry storage. eggs of ascaridia galli, the poultry ascarid, are also known to be highly resistant but the suitability as an indicator of pathogen inactivation has never been tested. pig slurry has to be stored for several months to inactivate pathogens but chemical treatment of slurry may reduce this time. the suitability of a. galli as an indicator of slurry sanita ... | 2014 | 24893691 |
| the conqueror worm: recent advances with cholinergic anthelmintics and techniques excite research for better therapeutic drugs. | the following account is based on a review lecture given recently at the british society of parasitology. we point out that nematode parasites cause very widespread infections of humans, particularly in economically underdeveloped areas where sanitation and hygiene are not adequate. in the absence of adequate clean water and effective vaccines, control and prophylaxis relies on anthelmintic drugs. widespread use of anthelmintics to control nematode parasites of animals has given rise to the deve ... | 2015 | 24871674 |
| immunoproteomic approach for identification of ascaris suum proteins recognized by pigs with porcine ascariasis. | ascaris suum, the causative agent of porcine ascariasis, is responsible for marked economic losses in pig farms worldwide. despite recent advances in research, including the characterization of the genome of a. suum, knowledge about the parasite/host relationship in porcine ascariasis at the molecular level is scarce and chemotherapy is the only effective option for parasite control. the aim of this study was to identify immunogenic proteins of a. suum somatic antigens associated with the pathog ... | 2014 | 24813788 |
| direct anthelmintic effects of condensed tannins from diverse plant sources against ascaris suum. | ascaris suum is one of the most prevalent nematode parasites in pigs and causes significant economic losses, and also serves as a good model for a. lumbricoides, the large roundworm of humans that is ubiquitous in developing countries and causes malnutrition, stunted growth and compromises immunity to other pathogens. new treatment options for ascaris infections are urgently needed, to reduce reliance on the limited number of synthetic anthelmintic drugs. in areas where ascaris infections are co ... | 2014 | 24810761 |
| advances in the diagnosis of ascaris suum infections in pigs and their possible applications in humans. | ascariasis is one of the most common parasitic diseases in both humans and pigs. it has been shown to cause growth deficits in both species and to impair cognitive development in children. notwithstanding its substantial impact on pig economy and public health, diagnosis of ascariasis has mostly relied on the detection of eggs in stool and further development of novel, more sensitive methods has been limited or non-existent. here, we discuss the currently available techniques for the diagnosis o ... | 2014 | 24775944 |
| comparative analysis of microrna profiles between adult ascaris lumbricoides and ascaris suum. | the parasitic nematodes ascaris lumbricoides and a. suum are of great public health and economic significance, and the two taxa were proposed to represent a single species. mirnas are known with functions of gene regulations at post-transcriptional level. | 2014 | 24766827 |
| functional study of a genetic marker allele associated with resistance to ascaris suum in pigs. | two single nucleotide polymorphisms (snp txnip and snp arnt), both on chromosome 4, have been reported to be associated with roundworm (ascaris suum) burden in pigs. in the present study, we selected pigs with two snp txnip genotypes (aa; n = 24 and ab; n = 24), trickle-infected them with a. suum from 8 weeks of age until necropsy 8 weeks later, and tested the hypothesis that pigs with the aa genotype would have higher levels of resistance than pigs of ab genotype. we used different indicators o ... | 2014 | 24709292 |
| immunosuppressive pas-1 is an excretory/secretory protein released by larval and adult worms of the ascarid nematode ascaris suum. | helminths use several strategies to evade and/or modify the host immune response, including suppression or inactivation of the host antigen-specific response. several helminth immunomodulatory molecules have been identified. our studies have focused on immunosuppression induced by the roundworm ascaris suum and an a. suum-derived protein named protein 1 from a. suum (pas-1). here we assessed whether pas-1 is an excretory/secretory (e/s) protein and whether it can suppress lipopolysaccharide-indu ... | 2015 | 24703095 |
| molecular epidemiology of ascariasis: a global perspective on the transmission dynamics of ascaris in people and pigs. | the roundworm ascaris lumbricoides infects 0.8 billion people worldwide, and ascaris suum infects innumerable pigs across the globe. the extent of natural cross-transmission of ascaris between pig and human hosts in different geographical settings is unknown, warranting investigation. | 2014 | 24688073 |
| effects of some pesticides on development of ascaris suum eggs. | to evaluate the effects of pesticides to parasite eggs, ascaris suum eggs were incubated with 5 different pesticides (1:1,500-1:2,000 dilutions of 2% emamectin benzoate, 5% spinetoram, 5% indoxacarb, 1% deltamethrin, and 5% flufenoxuron; all v/v) at 20℃ for 6 weeks, and microscopically evaluated the egg survival and development on a weekly basis. the survival rate of a. suum eggs incubated in normal saline (control eggs) was 90±3% at 6 weeks. however, the survival rates of eggs treated with pest ... | 2014 | 24623893 |
| hybrid ascaris suum/lumbricoides (ascarididae) infestation in a pig farmer: a rare case of zoonotic ascariasis. | we present a case of the 42-year-old pig farmer from the province of cuneo in northwest italy who was infected by the soil-transmitted nematode ascaris sp. in november 2010 the patient found one worm in his stool, subsequently identified as female specimen of ascaris sp. after a first anthelmintic treatment, another worm was found in his stool, that was later identified as male ascaris sp. blood tests prescribed by the patient's family physician, as suggested by a parasitologist, found nothing a ... | 2013 | 24592729 |
| evaluation of the use of c-terminal part of the schistosoma mansoni 200kda tegumental protein in schistosomiasis diagnosis and vaccine formulation. | schistosoma mansoni tegument is involved in essential functions for parasite survival and represents a target for screening candidates for vaccine and diagnosis. our group using reverse vaccinology selected six candidates, previously demonstrated by proteomics studies to be expressed in the parasite tegument, among them was sm200. in this work we have cloned and expressed a recombinant form of sm200 c-terminal (1069-1520) region. the efficacy of rsm200 (1069-1520) in the diagnosis of schistosomi ... | 2014 | 24560833 |
| treatment of canine asthma by high selective vagotomy. | this study evaluated the effect of high selective bilateral vagotomy of hilus pulmonis with video-assisted thoracoscopy on asthma. | 2014 | 24521967 |
| genome-wide tissue-specific gene expression, co-expression and regulation of co-expressed genes in adult nematode ascaris suum. | caenorhabditis elegans has traditionally been used as a model for studying nematode biology, but its small size limits the ability for researchers to perform some experiments such as high-throughput tissue-specific gene expression studies. however, the dissection of individual tissues is possible in the parasitic nematode ascaris suum due to its relatively large size. here, we take advantage of the recent genome sequencing of ascaris suum and the ability to physically dissect its separate tissue ... | 2014 | 24516681 |
| disorganized muscle protein-1 (dim-1) of filarial parasite brugia malayi: cdna cloning, expression, purification, structural modeling and its potential as vaccine candidate for human filarial infection. | we have recently identified disorganized muscle protein-1 (dim-1) in one of the proinflammatory fractions of the human filaria brugia malayi adult worm. the present study was undertaken to characterize b. malayi dim-1 (dim-1bm) and explore its vaccine potential. in this study we cloned and expressed the dim-1bm gene, investigated its sequence homology with other nematodes, constructed in silico structural model, purified the recombinant dim-1bm (rdim-1bm) protein, and studied the effect of immun ... | 2014 | 24513011 |
| transcription in pronuclei and one- to four-cell embryos drives early development in a nematode. | a long-standing view of development is that transcription is silenced in the oocyte until early divisions in the embryo. the point at which major transcription is reactivated varies between organisms, but is usually after the two-cell stage. however, this model may not be universal. | 2014 | 24374308 |
| complete mitochondrial genomes of chimpanzee- and gibbon-derived ascaris isolated from a zoological garden in southwest china. | roundworms (ascaridida: nematoda), one of the most common soil-transmitted helminths (sths), can cause ascariasis in various hosts worldwide, ranging from wild to domestic animals and humans. despite the veterinary and health importance of the ascaridida species, little or no attention has been paid to roundworms infecting wild animals including non-human primates due to the current taxon sampling and survey bias in this order. importantly, there has been considerable controversy over the years ... | 2013 | 24358225 |
| the intestinal expulsion of the roundworm ascaris suum is associated with eosinophils, intra-epithelial t cells and decreased intestinal transit time. | ascaris lumbricoides remains the most common endoparasite in humans, yet there is still very little information available about the immunological principles of protection, especially those directed against larval stages. due to the natural host-parasite relationship, pigs infected with a. suum make an excellent model to study the mechanisms of protection against this nematode. in pigs, a self-cure reaction eliminates most larvae from the small intestine between 14 and 21 days post infection. in ... | 2013 | 24340121 |
| in vitro evaluation of the ovistatic and ovicidal effect of the cosmopolitan filamentous fungi isolated from soil on ascaris suum eggs. | the ovicidal activity of seven fungal strains: acremonium alabamense, alternaria chlamydospora, cladosporium herbarum, fusarium solani, paecilomyces variotii, paecilomyces viridis and penicillium verruculosum isolated from urban soil samples from poland was determined in vitro. the fungal mycelium was co-cultured with ascaris suum eggs on plates with 2% water-agar for 28 days. eggs exposed and unexposed (control) to fungal mycelium were observed weekly by light microscopy and the percentage of m ... | 2014 | 24295958 |
| tlr2- and 4-independent immunomodulatory effect of high molecular weight components from ascaris suum. | components of high molecular-weight (pi) obtained from ascaris suum extract down-regulate the th1/th2-related immune responses induced by ovalbumin (ova)-immunization in mice. furthermore, the pi down-modulates the ability of dendritic cells (dcs) to activate t lymphocytes by an il-10-mediated mechanism. here, we evaluated the role of toll like receptors 2 and 4 (tlr2 and 4) in the modulatory effect of pi on ova-specific immune response and the pi interference on dc full activation. an inhibitio ... | 2014 | 24263181 |
| experimental infection of collared peccary ( dicotyles tajacu angulatus ) with swine kidney worm ( stephanurus dentatus ). | two captive-born juvenile collared peccaries ( dicotyles tajacu angulatus ) were given 3000 infective larvae of stephanurus dentatus per os. one peccary harbored viable s. dentatus sub-adults in the liver 50 days post-infection. the other peccary had no larvae but did have diffuse fibrotic hepatic lesions and bile duct hyperplasia 213 days post-infection; however, the lesions may have been partially due to a concurrent ascaris suum infection. a domestic pig ( sus scrofa domesticus ) infected as ... | 1977 | 24228971 |
| parasitic helminths of the digestive system of wild boars bred in captivity. | this study aimed to identify the parasites that inhabit the digestive system of sus scrofa scrofa from a commercial breeding facility in southern brazil, and reports the first occurrence of trichostrongylus colubriformis in wild boars. the gastrointestinal tracts of 40 wild boars from a commercial breeding facility were collected and individualized during slaughter in a cold-storage slaughterhouse. out of this total, 87.5% were parasitized by the helminths ascaris suum, trichostrongylus colubrif ... | 2013 | 24142179 |
| proteomic analysis of the excretory-secretory products from larval stages of ascaris suum reveals high abundance of glycosyl hydrolases. | ascaris lumbricoides and ascaris suum are socioeconomically important and widespread parasites of humans and pigs, respectively. the excretory-secretory (es) molecules produced and presented at the parasite-host interface during the different phases of tissue invasion and migration are likely to play critical roles in the induction and development of protective immune and other host responses. | 2013 | 24098821 |
| potential of recombinant inorganic pyrophosphatase antigen as a new vaccine candidate against baylisascaris schroederi in mice. | the intestinal nematode baylisascaris schroederi is an important cause of death for wild and captive giant pandas. inorganic pyrophosphatases (ppases) are critical for development and molting in nematode parasites and represent potential targets for vaccination. here, a new ppase homologue, bsc-pyp-1, from b. schroederi was identified and characterized, and its potential as a vaccine candidate was evaluated in a mouse challenge model. sequence alignment of ppases from nematode parasites and othe ... | 2013 | 24090087 |
| sequence variability in three mitochondrial genes among four roundworm species from wild animals in china. | sequence variability in three mitochondrial dna (mtdna) regions, namely portions of cytochrome c oxidase subunit 1 (pcox1), nadh dehydrogenase subunit 1 (pnad1) and nadh dehydrogenase subunit 4 (pnad4), for toxocara canis. baylisacaris transfuga. ascaris suum and parascaris equorum from canis lupus. ursus thibetanus. sus scrofa and equus burchelli in china were examined. the lengths of the sequences of pcox1, pnad1 and pnad4 were 711 bp, 648 bp and 666 bp, respectively. no intra-species differen ... | 2015 | 24041450 |
| modeling the die-off of e. coli and ascaris in wastewater-irrigated vegetables: implications for microbial health risk reduction associated with irrigation cessation. | this study assessed the die-off of escherichia coli (e. coli) and ascaris suum on lettuce (great lakes 118) and cabbage (brassica oleracea var capitata) in wastewater-irrigated fields using comparative mathematical die-off models. the study revealed that none of the survival curves of e. coli and a. suum was best fitted with the log-linear model, indicating that the classical first-order kinetic approach is inadequate in many cases. the biphasic die-off model best described the die-off of e. col ... | 2013 | 24037151 |
| elevated guanylate cyclase and cyclic-guanosine monophosphate-dependent protein kinase levels in nasal mucosae of antigen-challenged rats. | in patients with severe allergic rhinitis, the most serious symptom is rhinostenosis, which is considered to be induced by a dilatation of plexus cavernosum. the vascular relaxing responses to chemical mediators are mainly mediated by the production of nitric oxide (no). however, the exact mechanism(s) in nasal venoresponsiveness of allergic rhinitis is not fully understood. in the present study, we investigated the roles of soluble guanylate cyclase (sgc) and cyclic-guanosine monophosphate (c-g ... | 2013 | 24012634 |
| follow-up study of prevalence and control of ascariasis in swine populations in serbia. | the cause of the most significant helminth diseases in swine - ascaris suum, can also causes infections in humans. the use of swine manure in agriculture renders the eggs of this ascaridida an accessible source of infection, thus posing a significant risk factor for human health. with the objective of proving the prevalence of infection with a. suum nematodes in the territory of serbia, investigations were carried out in the period between 2007-2011 in the territories of five districts: north ba ... | 2013 | 23990423 |
| analysis of the accuracy and precision of the mcmaster method in detection of the eggs of toxocara and trichuris species (nematoda) in dog faeces. | the aim of this study was to determine the accuracy and precision of mcmaster method with raynaud's modification in the detection of the eggs of the nematodes toxocara canis (werner, 1782) and trichuris ovis (abildgaard, 1795) in faeces of dogs. four variants of mcmaster method were used for counting: in one grid, two grids, the whole mcmaster chamber and flotation in the tube. one hundred sixty samples were prepared from dog faeces (20 repetitions for each egg quantity) containing 15, 25, 50, 1 ... | 2013 | 23951934 |
| the potential for self-sanitisation of faecal sludge by intrinsic ammonia. | faecal sludge has the potential to be used as a sustainable fertiliser in agriculture, but the sludge must be sanitised due to its content of pathogenic microorganisms. the intrinsic ammonia from the urine may be sufficient for sanitisation of the sludge if it is not too diluted by flush water or lost by ventilation. to evaluate the potential for this sanitisation method, inactivation of enterococcus faecalis, salmonella typhimurium and ascaris suum eggs during treatment were assessed. the inact ... | 2013 | 23941983 |
| bacillus thuringiensis-derived cry5b has potent anthelmintic activity against ascaris suum. | ascaris suum and ascaris lumbricoides are two closely related geo-helminth parasites that ubiquitously infect pigs and humans, respectively. ascaris suum infection in pigs is considered a good model for a. lumbricoides infection in humans because of a similar biology and tissue migration to the intestines. ascaris lumbricoides infections in children are associated with malnutrition, growth and cognitive stunting, immune defects, and, in extreme cases, life-threatening blockage of the digestive t ... | 2013 | 23818995 |
| efficacy of ivermectin and oxfendazole against taenia solium cysticercosis and other parasitoses in naturally infected pigs. | smallholder semi-confined pig production is a fast growing practice in sub-saharan africa with an unfortunate outcome of high prevalence of taenia solium cysticercosis and other parasitoses. the widely used anthelmintic for control of endo and ecto-parasites in pigs in the area is ivermectin at a recommended dose of 0.3mg/kg. this study was conducted to evaluate the efficacy and safety in pigs after subcutaneous injection of ivermectin (ivm, 0.3mg/kg) and orally administration of oxfendazole (of ... | 2013 | 23806569 |
| a review of pig pathology in tanzania. | the approximately 1.58 million pigs in tanzania represent 3.7% of the national population of quadruped meat-producing animals. pigs are kept mainly by small producers who own 99.5% of the national stock in units that average 3.04 animals (range 2-48). government policy has had little practical application. african swine fever, foot-and-mouth disease and cysticercosis are important diseases. the first two are notifiable diseases under tanzania legislation; the last has widespread distribution and ... | 2013 | 23733144 |
| timps of parasitic helminths - a large-scale analysis of high-throughput sequence datasets. | tissue inhibitors of metalloproteases (timps) are a multifunctional family of proteins that orchestrate extracellular matrix turnover, tissue remodelling and other cellular processes. in parasitic helminths, such as hookworms, timps have been proposed to play key roles in the host-parasite interplay, including invasion of and establishment in the vertebrate animal hosts. currently, knowledge of helminth timps is limited to a small number of studies on canine hookworms, whereas no information is ... | 2013 | 23721526 |
| cytosolic ca(2+) as a multifunctional modulator is required for spermiogenesis in ascaris suum. | the dynamic polar polymers actin filaments and microtubules are usually employed to provide the structural basis for establishing cell polarity in most eukaryotic cells. radially round and immotile spermatids from nematodes contain almost no actin or tubulin, but still have the ability to break symmetry to extend a pseudopod and initiate the acquisition of motility powered by the dynamics of cytoskeleton composed of major sperm protein (msp) during spermiogenesis (sperm activation). however, the ... | 2013 | 23686736 |
| faecal sludge management with the larvae of the black soldier fly (hermetia illucens)--from a hygiene aspect. | inadequate and lacking sanitation and wastewater treatment systems can lead to the spreading of diarrhoeal diseases. one contributing factor in the lack of such treatment systems is the lack of economic incentives for stakeholders throughout the service chain. however, the organic fraction of the waste is high in valuable plant nutrients and could be reused in agriculture and as animal feed. for example, grown larvae of the black soldier fly, hermetia illucens l. (diptera: stratiomyidae), make a ... | 2013 | 23669577 |
| withdrawn: co-administration of ccl5 and il4 enhances dna vaccine-induced protective immunity to ascaris suum infection in mice. | this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2013 | 23597869 |
| gene expression analysis distinguishes tissue-specific and gender-related functions among adult ascaris suum tissues. | over a billion people are infected by ascaris spp. intestinal parasites. to clarify functional differences among tissues of adult a. suum, we compared gene expression by various tissues of these worms by expression microarray methods. the a. suum genome was sequenced and assembled to allow generation of microarray elements. expression of over 40,000 60-mer elements was investigated in a variety of tissues from both male and female adult worms. nearly 50 percent of the elements for which signal w ... | 2013 | 23572074 |
| a role for eosinophils in the intestinal immunity against infective ascaris suum larvae. | the aim of this study was to explore the mechanisms of resistance against invading ascaris suum larvae in pigs. pigs received a low dose of 100 a. suum eggs daily for 14 weeks. this resulted in a >99% reduction in the number of larvae that could migrate through the host after a challenge infection of 5000 a. suum eggs, compared to naïve pigs. histological analysis at the site of parasite entry, i.e. the caecum, identified eosinophilia, mastocytosis and goblet cell hyperplasia. increased local tr ... | 2013 | 23556022 |
| vaccination of goats with dna vaccine encoding dim-1 induced partial protection against haemonchus contortus: a preliminary experimental study. | disorganized muscle family member (dim-1) belongs to immunoglobulin superfamily, and is a structural protein localized to the region of the muscle cell membrane around and between the dense bodies. strong immunogenicity to host's immune system was induced by dim-1 from ascaris suum, which indicated that dim-1 could be a potential candidate for vaccine. the homologues of dim-1 were also detected in nematodes brugia malayi and trichostrongylus colubriformis. however, information on the complete co ... | 2013 | 23545480 |
| investigation of parasitic and bacterial diseases in pigs with analysis of hematological and serum biochemical profile. | the present study was undertaken to evaluate various disease conditions prevalent in slaughtered pigs and zoonotic importance. the study was conducted on two hundred non-descript pigs slaughtered at an organized slaughter house, mumbai. the animals included in the study were randomly selected. post mortem examination of the animals was performed to note various disease conditions and tissues were collected for histopathology. direct examination of stool was found negative for parasites. gross an ... | 2011 | 23542948 |
| effects of spring season solar drying process on sanitation indicators in sewage sludge and potential as a method for fertilizer production. | the agricultural use of sewage sludge is possible on condition of maintaining microbiological and parasitological standards, and one of the most modern methods improving its sanitary state is solar drying. in the presented study, the effect of this process on the elimination of indicator microorganisms (escherichia coli, salmonella senftenberg w775, enterococcus spp.) and eggs of ascaris suum introduced into the biomass of sludge was examined. the experiment was carried out in the spring period ... | 2013 | 23540205 |
| biological interactions between soil saprotrophic fungi and ascaris suum eggs. | the in vitro effect of saprotrophic soil fungi on the embryonic development of ascaris suum was evaluated. the fungi tested were aspergillus fumigatus, aspergillus terreus, penicillium citrinum, penicillium expansum, fusarium oxysporum and trichothecium roseum, isolated from children's recreation areas in the city of lodz (poland). each species was co-cultured with a. suum egg suspension (6 × 10(3)eggs/ml) at 25 ± 2°c for 60 days. each day, 100 eggs were randomly collected and their developmenta ... | 2013 | 23534981 |
| derquantel and abamectin: effects and interactions on isolated tissues of ascaris suum. | startect(®) is a novel anthelmintic combination of derquantel and abamectin. it is hypothesized that derquantel and abamectin interact pharmacologically. we investigated the effects of derquantel, abamectin and their combination on somatic muscle nicotinic acetylcholine receptors and pharyngeal muscle glutamate gated chloride receptor channels of ascaris suum. we used muscle-strips to test the effects of abamectin, derquantel, and abamectin+derquantel together on the contraction responses to dif ... | 2013 | 23523993 |
| three independent techniques localize expression of transcript afp-11 and its bioactive peptide products to the paired avk neurons in ascaris suum : in situ hybridization, immunocytochemistry, and single cell mass spectrometry. | we utilized three independent techniques, immunocytochemistry (icc), single cell mass spectrometry (ms), and in situ hybridization (ish), to localize neuropeptides and their transcripts in the nervous system of the nematode ascaris suum . af11 (sdigisepnflrfa) is an endogenous peptide with potent paralytic effects on a. suum locomotory behavior. a highly specific antibody to af11 showed robust immunostaining for af11 in the paired avk neurons in the ventral ganglion. we traced the processes from ... | 2012 | 23509978 |
| nicotinic acetylcholine receptors: a comparison of the nachrs of caenorhabditis elegans and parasitic nematodes. | nicotinic acetylcholine receptors (nachrs) play a key role in the normal physiology of nematodes and provide an established target site for anthelmintics. the free-living nematode, caenorhabditis elegans, has a large number of nachr subunit genes in its genome and so provides an experimental model for testing novel anthelmintics which act at these sites. however, many parasitic nematodes lack specific genes present in c. elegans, and so care is required in extrapolating from studies using c. ele ... | 2013 | 23500392 |
| inactivation of ascaris suum eggs. | 2013 | 23489736 | |
| antagonism of chemokine receptor ccr8 is ineffective in a primate model of asthma. | expression of the t-cell-associated chemokine receptor ccr8 and its ligand ccl1 have been demonstrated to be elevated in patients with asthma. ccr8 deficiency or inhibition in models of allergic airway disease in mice resulted in conflicting data. | 2013 | 23457038 |
| an anomaly within the genital system in a female ascaris suum goeze. | the authors describe an individual of the female ascaris suum goeze with a unique genital system. a female with such an anomaly was found during laboratory classes of invertebrate zoology at the faculty of biology and environmental protection, ncu. the specimen was about 200 mm long, and the total length of the reproductive system was 1970 mm. further comparative analysis between unchanged and changed individuals revealed differences in the length of individual sections of the studied system, as ... | 2012 | 23444801 |
| efficacy of a single high oxfendazole dose against gastrointestinal nematodes in naturally infected pigs. | the goal of the current experiment was to assess the clinical efficacy of oxfendazole (ofz) administered as a single oral dose (30 mg/kg) to pigs naturally parasitized with ascaris suum, oesophagostomum spp., metastrongylus spp. and trichuris suis. thirty-six local ecotype piglets were divided into three independent experiments, named i, ii and iii (n=12 each), respectively. each experiment involved two different groups (n=6): untreated control and ofz treated. animals were naturally parasitized ... | 2013 | 23357598 |
| inactivation of pathogens in feces by desiccation and urea treatment for application in urine-diverting dry toilets. | ecological sanitation technologies can be effective in providing health and environmental pollution control if they can efficiently reduce the pathogenicity of microorganisms carried in fecal material to safe levels. this study evaluated the sanitizing effects of different additives for dry treatment of feces from urine-diverting dry toilets, based on inactivation of enterococcus faecalis, salmonella enterica serovar typhimurium, bacteriophages ms2 and φx, and ascaris suum. the additives, ash (a ... | 2013 | 23335764 |
| diversity of parasite complex ii. | parasites have developed a variety of physiological functions necessary for completing at least part of their life cycles in the specialized environments of surrounding the parasites in the host. regarding energy metabolism, which is essential for survival, parasites adapt to the low oxygen environment in mammalian hosts by using metabolic systems that are very different from those of the hosts. in many cases, the parasite employs aerobic metabolism during the free-living stage outside the host ... | 2013 | 23333273 |
| comparative profiling of micrornas in male and female adults of ascaris suum. | ascaris nematodes, which cause ascariasis in humans and pigs, are among the most important nematodes from both health and economic perspectives. microrna (mirna) is now recognized as key regulator of gene expression at posttranscription level. the public availability of the genome and transcripts of ascaris suum provides powerful resources for the research of mirna profiles of the parasite. therefore, we investigated and compared the mirna profiles of male and female adult a. suum using solexa d ... | 2013 | 23306386 |
| cloning and characterization of hypoxia-inducible factor-1 subunits from ascaris suum - a parasitic nematode highly adapted to changes of oxygen conditions during its life cycle. | the parasitic nematode ascaris suum successfully adapts to a significant decrease in oxygen availability during its life cycle by altering its metabolic system dramatically. however, little is known about the regulatory mechanisms of adaptation to hypoxic environments in a. suum. in multicellular organisms, hypoxia-inducible factor-1 (hif-1), a heterodimeric transcription factor composed of hif-1α and hif-1β subunits, is a master regulator of genes involved in adaptation to hypoxia. in the prese ... | 2012 | 23268347 |
| survival of weed seeds and animal parasites as affected by anaerobic digestion at meso- and thermophilic conditions. | anaerobic digestion of residual materials from animals and crops offers an opportunity to simultaneously produce bioenergy and plant fertilizers at single farms and in farm communities where input substrate materials and resulting digested residues are shared among member farms. a surplus benefit from this practice may be the suppressing of propagules from harmful biological pests like weeds and animal pathogens (e.g. parasites). in the present work, batch experiments were performed, where survi ... | 2012 | 23266071 |
| resonance assignment of as-p18, a fatty acid binding protein secreted by developing larvae of the parasitic nematode ascaris suum. | as-p18 is produced and secreted by larvae of the parasitic nematode ascaris suum as they develop within their eggs. the protein is a member of the fatty acid binding protein (fabp) family found in a wide range of eukaryotes, but is distinctive in that it is secreted from the synthesizing cell and has predicted additional structural features not previously seen in other fabps. as-p18 and similar proteins found only in nematodes have therefore been designated 'nemfabps'. sequence-specific (1)h, (1 ... | 2014 | 23225165 |
| parasites of the respiratory tract of sus scrofa scrofa (wild boar) from commercial breeder in southern brazil and its relationship with ascaris suum. | this study aimed to identify the species of helminths infecting the respiratory tract of sus scrofa scrofa from commercial breeding and check the existence of a possible antagonistic relationship of these species with ascaris suum. forty wild boars were analyzed, and the genus metastrongylus was recorded in the bronchi and bronchioles of 60 % of these, with the occurrence of the species metastrongylus apri, metastrongylus salmi, and metastrongylus pudendotectus. the highest prevalence found was ... | 2013 | 23224612 |
| duplex quantitative real-time pcr assay for the detection and discrimination of the eggs of toxocara canis and toxocara cati (nematoda, ascaridoidea) in soil and fecal samples. | toxocarosis is a zoonotic disease caused by toxocara canis (t. canis) and/or toxocara cati (t. cati), two worldwide distributed roundworms which are parasites of canids and felids, respectively. infections of humans occur through ingestion of embryonated eggs of t. canis or t. cati, when playing with soils contaminated with dogs or cats feces. accordingly, the assessment of potential contamination of these areas with these roundworms eggs is paramount. | 2012 | 23216873 |
| identification and characterization of new major sperm protein genes from oesophagostomum dentatum and oesophagostomum quadrispinulatum from pigs in china. | the present study identified and characterized new major sperm protein (msp) genes from the two nodule worms oesophagostomum dentatum and oesophagostomum quadrispinulatum collected from pigs in china. total genomic dna was extracted individually from 10 male nematode samples representing o. dentatum, and 4 male nematode samples representing o. quadrispinulatum. a pair of primers (omsp1f/msp1r) was designed based on the msp gene sequences of ascaris suum and o. dentatum available in genbank, and ... | 2013 | 23206956 |
| inhibitory effect of topical adelmidrol on antigen-induced skin wheal and mast cell behavior in a canine model of allergic dermatitis. | adelmidrol is a semisynthetic derivative of azelaic acid and analogue of the anti-inflammatory compound palmitoylethanolamide (pea). based upon its physicochemical properties, adelmidrol is suitable for topical application. the main objective of the present study was to evaluate the efficacy of a topical adelmidrol emulsion on early and late inflammatory responses in hypersensitive dogs. repeated intradermal injections of ascaris suum extract were performed in both lateral thoracic areas of six ... | 2012 | 23181761 |
| viability of ascaris suum eggs in stored raw and separated liquid slurry. | separation of pig slurry into solid and liquid fractions is gaining importance as a way to manage increasing volumes of slurry. in contrast to solid manure and slurry, little is known about pathogen survival in separated liquid slurry. the viability of ascaris suum eggs, a conservative indicator of fecal pollution, and its association with ammonia was investigated in separated liquid slurry in comparison with raw slurry. for this purpose nylon bags with 6000 eggs each were placed in 1 litre bott ... | 2013 | 23127297 |
| silencing of germline-expressed genes by dna elimination in somatic cells. | chromatin diminution is the programmed elimination of specific dna sequences during development. it occurs in diverse species, but the function(s) of diminution and the specificity of sequence loss remain largely unknown. diminution in the nematode ascaris suum occurs during early embryonic cleavages and leads to the loss of germline genome sequences and the formation of a distinct genome in somatic cells. we found that ∼43 mb (∼13%) of genome sequence is eliminated in a. suum somatic cells, inc ... | 2012 | 23123092 |
| biolistic transformation of brugia malayi. | biolistics has become a versatile tool for direct gene transfer to various cell and tissue types. following its successful use on the parasitic nematode ascaris suum, we developed and evaluated biolistics in the transfection of the model filarial parasite brugia malayi. biolistics was proven to be an efficient strategy for transfection of all life stages of the parasite and paved the way for studies on elements essential for promoter function and gene regulation of filarial parasites. here we pr ... | 2013 | 23104337 |
| ineffectiveness of a quaternary ammonium salt and povidone-iodine for the inactivation of ascaris suum eggs. | two commonly used disinfectants, a quaternary ammonium salt and povidone-iodine, were tested for effectiveness against unembryonated ascaris suum eggs. the quaternary ammonium salt (alkyl dimethyl benzyl ammonium chloride) had no effect on the ascaris eggs (10 minutes and 22°c) when compared with the controls in water with egg viabilities of 88.8% ± 3.3% and 86.9% ± 6.2%, respectively. an additional quaternary ammonium salt, 2.5% benzethonium chloride, also had no effect. phenol (5%) and cresol ... | 2013 | 23102984 |
| effect of oral and intravenous heparin tetrasaccharide on allergic airway responses: critical role of n-sulfation. | we have shown that inhaled heparin (hep) oligosaccharides attenuate allergic airway responses in sheep and that this anti-allergic activity resides in a tetrasaccharide sequence. here we determined: (a) the anti-allergic activity of oral and intravenous hep-tetrasaccharide on allergic airway responses in the sheep model of asthma; and (b) the role of n-sulfation in mediating this anti-allergic activity. ascaris suum-induced early (ear) and late (lar) airway responses and airway hyperresponsivene ... | 2013 | 23085243 |
| detailed transcriptome description of the neglected cestode taenia multiceps. | the larval stage of taenia multiceps, a global cestode, encysts in the central nervous system (cns) of sheep and other livestock. this frequently leads to their death and huge socioeconomic losses, especially in developing countries. this parasite can also cause zoonotic infections in humans, but has been largely neglected due to a lack of diagnostic techniques and studies. recent developments in next-generation sequencing provide an opportunity to explore the transcriptome of t. multiceps. | 2012 | 23049872 |
| trichinella spiralis: monoclonal antibody against the muscular larvae for the detection of circulating and fecal antigens in experimentally infected rats. | in this work we search for antigens of trichinella spiralis in sera and stool of rats experimentally infected. the kinetic of antibodies to excretory and secretory (es) antigens of muscle larvae (ml) was also determined. wistar rats were infected with 15 ml per gram of body weight and blood samples were collected weekly for 10 weeks. antibodies were studied using an indirect elisa. for detection of circulating antigens and coproantigens, a sandwich elisa was developed with the use of polyclonal ... | 2012 | 23026455 |
| inactivation kinetics of cryptosporidium parvum oocysts in a swine waste lagoon and spray field. | because of outbreaks of cryptosporidiosis in humans, some cryptosporidium spp. have become a public health concern. commercial swine operations can be a source of this protozoan parasite. although the species distribution of cryptosporidium is likely dominated by cryptosporidium suis , a fraction may be comprised of other cryptosporidium species infectious to humans such as cryptosporidium parvum . to better understand the survival dynamics of cryptosporidium spp., oocysts associated with swine ... | 2013 | 23016982 |
| quantitative evaluation of viability- and apoptosis-related genes in ascaris suum eggs under different culture-temperature conditions. | ascaris suum eggs are inactivated by composting conditions; however, it is difficult to find functional changes in heat-treated a. suum eggs. here, unembryonated a. suum eggs were incubated at 20°c, 50°c, and 70°c in vitro, and the gene expression levels related to viability, such as eukaryotic translation initiation factor 4e (if4e), phosphofructokinase 1 (pfk1), and thioredoxin 1 (trx1), and to apoptosis, such as apoptosis-inducing factor 1 (aif1) and cell death protein 6 (cdp6), were evaluate ... | 2012 | 22949754 |
| effect of temperature on embryonation of ascaris suum eggs in an environmental chamber. | the influence of temperature on the development and embryonation of ascaris suum eggs was studied using coarse sand medium in an environmental chamber with 50% humidity. the time required for development and embryonation of eggs was examined under 3 different temperature conditions, 5°c, 25°c, and 35°c. a. suum eggs did not develop over 1 month at the temperature of 5°c. however, other temperature conditions, 25°c and 35°c, induced egg development to the 8-cell-stage at days 5-6 after incubation ... | 2012 | 22949753 |
| identifying associations between pig pathologies using a multi-dimensional machine learning methodology. | abattoir detected pathologies are of crucial importance to both pig production and food safety. usually, more than one pathology coexist in a pig herd although it often remains unknown how these different pathologies interrelate to each other. identification of the associations between different pathologies may facilitate an improved understanding of their underlying biological linkage, and support the veterinarians in encouraging control strategies aimed at reducing the prevalence of not just o ... | 2012 | 22937883 |
| transformation: how do nematode sperm become activated and crawl? | nematode sperm undergo a drastic physiological change during spermiogenesis (sperm activation). unlike mammalian flagellated sperm, nematode sperm are amoeboid cells and their motility is driven by the dynamics of a cytoskeleton composed of major sperm protein (msp) rather than actin found in other crawling cells. this review focuses on sperm from caenorhabditis elegans and ascaris suum to address the roles of external and internal factors that trigger sperm activation and power sperm motility. ... | 2012 | 22903434 |
| feasibility study for the detection of trichinella spiralis in a murine model using mid-fourier transform infrared spectroscopy (mid-ftir) with attenuated total reflectance (atr) and soft independent modelling of class analogies (simca). | fourier transform infrared (ftir) spectroscopy with attenuated total reflectance (atr) and soft independent modelling by class analogies (simca) was used to assess the feasibility of detecting trichinella spiralis in a murine model. the selected ftir wavenumber range was 1700-900 cm(-1) and the first derivative of the spectra was subjected to simca analysis. the simca model developed for rat meat spiked with t. spiralis larvae was successfully apply to classify non-infected from infected rat mea ... | 2012 | 22874013 |
| useable diffraction data from a multiple microdomain-containing crystal of ascaris suum as-p18 fatty-acid-binding protein using a microfocus beamline. | as-p18 is a fatty-acid-binding protein from the parasitic nematode ascaris suum. although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. crystals were obtained, but initial diffraction data analysis revealed that they were composed of a number of `microdomains'. interpretable data could only be collected using a microfocus beamline with a beam size of 12 × 8 µm. | 2012 | 22869127 |