Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| detection of the carcinogenic liver fluke opisthorchis viverrini using a mini parasep sf faecal parasite concentrator. | the mini parasep sf fecal parasite concentrator (mpsfc) is a new modification of the closed concentration system, which can easily be adopted in any routine clinical pathology laboratory. here we describe our experience with the system in diagnosing opisthorchis viverrini. a total of 199 fecal samples was submitted for routine examination in the clinical pathology laboratory of suranaree university of technology hospital, nakhon ratchasima province, thailand, during august to october 2015. out o ... | 2016 | 26838241 |
| helicobacter species are possible risk factors of cholangiocarcinoma. | several infectious agents are considered to be causes of cancer in human, mainly hepatitis b and c viruses, high-risk human pailloma viruses, helicobacter pylori, clonorchis sinensis, and opisthorchis viverrini. here we described the evident research and the association between helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (cca). global epidemiological studies have suggested that helicobacter spp. are possible risk factors for biliary tract diseases. molecular studie ... | 2016 | 26838240 |
| serum antibody ig g and ig m titers for opisthorchis felineus correlate with eggs in faeces--a comprehensive study in chuvash republic, russia. | the cholangiocarcinoma is a. the risk of development of cholangiocarcinoma, generally a rare type of a liver tumor, increases during infection of opisthorchiasis. for this reason the timely detection of opisthorchiasis is important for cholangiocarcinoma prevention. there are many studies which concern the detection of pathogenesis of opisthorchis viverrini infection but a little known about opisthorchis felineus. in this study we investigate a correlation of the eggs which are found in a faeces ... | 2016 | 26838224 |
| re-examination of opisthorchis viverrini in nakhon ratchasima province, northeastern thailand, indicates continued needs for health intervention. | opisthorchis viverrini infection is associated with cholangiocarcinoma particularly in the cases of chronic or re-infection. this presents a serious health problem in northeastern and northern thailand. a community base approach is required for surveillance. therefore, in a pilot project, re-examination of o. viverrini infection was conducted in the 3 districts of nakhon ratchasima province, thailand, during june and october 2015. a total of 355 participants from a 194,152 population, was select ... | 2016 | 26838215 |
| people participation towards opisthorchis viverrini prevention and control in chaiyaphum province, northeastern thailand. | this cross-sectional descriptive and qualitative study was aimed to study the people participation and their approaches toward the human carcinogenic liver fluke, opisthorchis viverrini, prevention and control in ban chaun sub-district administrative organization (bcsao) and bamnet narong sub-district municipality (bnsm), bamnet narong district, chaiyaphum province, thailand between june 2013 and february 2014. participants were purposive selected, included chiefs of sub-district administrative ... | 2016 | 26838206 |
| untangling the complexity of liver fluke infection and cholangiocarcinoma in ne thailand through transdisciplinary learning. | this study demonstrates how a transdisciplinary learning approach provided new insights for explaining persistent opisthorchis viverrini infection in northern thailand, as well as elucidating problems of focusing solely on the parasite as a means of addressing high prevalence of cholangiocarcinoma. researchers from diverse backgrounds collaborated to design an investigative homestay program for 72 singaporean and thai university students in five northeast thai villages. the students explored how ... | 2016 | 26822781 |
| andrographolide ameliorates beta-naphthoflavone-induced cyp1a enzyme activity and lipid peroxidation in hamsters with acute opisthorchiasis. | opisthorchis viverrini (ov) infection generates oxidative stress/free radicals and is considered as a primary cause ofcholangiocarcinoma since it primarily triggers sclerosing cholangitis. | 2016 | 26817235 |
| hemozoin is a product of heme detoxification in the gut of the most medically important species of the family opisthorchiidae. | many species of trematodes such as schistosoma spp., fasciola hepatica and echinostoma trivolvis are blood-feeding parasites. nevertheless, there is no consensus on the feeding habits of the family opisthorchiidae (opisthorchis felineus, opisthorchis viverrini and clonorchis sinensis). previously, histological studies of o. felineus and c. sinensis revealed some dark stained material in their gut lumen. in this study we conducted a comprehensive analysis of the gut contents of three members of t ... | 2016 | 26812025 |
| the complete mitochondrial genome of metorchis orientalis (trematoda: opisthorchiidae): comparison with other closely related species and phylogenetic implications. | metorchis orientalis (trematoda: opisthorchiidae) is an important trematode infecting many animals and humans, causing metorchiasis. in the present study, the complete mitochondrial (mt) genome of m. orientalis was sequenced. the complete mt genome of m. orientalis is 13,834 bp circular dna molecule and contains 12 protein-coding genes, 22 transfer rna genes, and two ribosomal rna genes. the gene content and arrangement of m. orientalis is the same as those of opisthorchiidae trematodes (opistho ... | 2016 | 26805437 |
| xanthohumol inhibits stat3 activation pathway leading to growth suppression and apoptosis induction in human cholangiocarcinoma cells. | stat3 plays a significant role in the development of cholangiocarcinoma (cca) associated with the liver fluke (opisthorchis viverrini; ov). xanthohumol (xn), a prenylated flavonoid extracted from hops, has known anticancer activity and could potentially target stat3. the present study determined the effect of xn on stat3, as well as ascertained its usefulness against cca. the cca cell proliferation at 20 µm and 50 µm of xn was shown to inhibited, while 20 µm partially inhibited il-6-induced stat ... | 2016 | 26794001 |
| phylogenetic characterization of clonorchis sinensis proteins homologous to the sigma-class glutathione transferase and their differential expression profiles. | glutathione transferase (gst) is one of the major antioxidant proteins with diverse supplemental activities including peroxidase, isomerase, and thiol transferase. gsts are classified into multiple classes on the basis of their primary structures and substrate/inhibitor specificity. however, the evolutionary routes and physiological environments specific to each of the closely related bioactive enzymes remain elusive. the sigma-like gsts exhibit amino acid conservation patterns similar to the pr ... | 2016 | 26792248 |
| genomics of worms, with an emphasis on opisthorchis viverrini - opportunities for fundamental discovery and biomedical outcomes. | neglected tropical diseases cause substantial morbidity and mortality in animals and people globally. opisthorchiasis is one such disease, caused by the carcinogenic, asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is known to be associated with malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia, including thailand, lao people's democratic republic (pdr) and cambodia. no vaccine is available, and only one drug (praziquantel) is routinely emplo ... | 2016 | 26792076 |
| comparison of infectivity, metacercarial burden and host mortality induced by opisthorchis viverrini sensu lato cercariae from lao pdr compared with thailand in cyprinid fish, barbonymus gonionotus. | previous studies indicate that opisthorchis viverrini sensu lato (s.l.) is a species complex with strong associations to geographical regions, i.e., specific wetlands. the present study was conducted to compare the infectivity, establishment and metacercarial burden and survival of the fish host following exposure to o. viverrini s.l. cercariae isolates from two different river wetlands. | 2016 | 26740362 |
| clonorchis sinensis and opisthorchis spp. in vietnam: current status and prospects. | clonorchis sinensis and opisthorchis viverrini are clinically important small liver flukes because of their known association with development of cholangiocarcinoma. in vietnam, high prevalence of c. sinensis infection in humans was previously reported in northern provinces, and o. viverrini infection has been detected in several central provinces. however, diagnosis of c. sinensis and o. viverrini infections in the past was merely based on faecal egg examination. this method alone can lead to m ... | 2016 | 26740358 |
| increase in l-type amino acid transporter 1 expression during cholangiocarcinogenesis caused by liver fluke infection and its prognostic significance. | l-type amino acid transporter 1 (lat1) is highly expressed in various human cancers, including cholangiocarcinoma (cca), the most common cancer in northeast thailand. chronic inflammation and oxidative stress induced by liver fluke, opisthorchis viverrini, infection has been recognized as the major cause of cca in this area. we show here that an increased expression of lat1 and its co-functional protein cd98 are found during carcinogenesis induced by ov in hamster cca tissues. we also demonstrat ... | 2015 | 26657242 |
| the carcinogenic liver fluke opisthorchis viverrini among rural community people in northeast thailand: a cross- sectional descriptive study using multistage sampling technique. | opisthorchis viverrini infection is a serious public health problem in southeast asia especially in the northeast and north of thailand. therefore, a cross-sectional survey using multistage sampling was conducted from the rural communities of surin province, thailand, during september 2013 to july 2014. o. viverrini infection was determined using kato's thick smear technique. socio-demographic, information resources, and history data were collected using predesigned semi-structured questionnaire ... | 2015 | 26625801 |
| functional analysis of the unique cytochrome p450 of the liver fluke opisthorchis felineus. | the basic metabolic cytochrome p450 (cyp) system is essential for biotransformation of sterols and xenobiotics including drugs, for synthesis and degradation of signaling molecules in all living organisms. most eukaryotes including free-living flatworms have numerous paralogues of the cyp gene encoding heme monooxygenases with specific substrate range. notably, by contrast, the parasitic flatworms have only one cyp gene. the role of this enzyme in the physiology and biochemistry of helminths is ... | 2015 | 26625139 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsist ... | 2015 | 26618199 |
| effects of fermentation time and low temperature during the production process of thai pickled fish (pla-som) on the viability and infectivity of opisthorchis viverrini metacercariae. | contamination of a popular fermented fish dish, pla-som, by opisthorchis viverrini metacercariae (ovmc) is a possible cause of carcinogenic liver fluke infection in thailand. affected individuals are at risk of bile duct cancer, which is a major health problem for people in the greater mekong subregion. in order to investigate concerns about food safety, we studied the effects of fermentation time and low temperature on the viability and infectivity of ovmc during the pla-som production process. ... | 2016 | 26587967 |
| nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in opisthorchis viverrini-infected hamsters. | this study investigated the effects of nanoencapsulated curcumin (nec) and praziquantel (pzq) treatment on the resolution of periductal fibrosis (pdf) and bile canalicular (bc) abnormalities in opisthorchis viverrini infected hamsters. chronic o. viverrini infection (ov) was initially treated with either pzq (op) and subsequently treated with nec (op+nec), curcumin (op+cur) or unloaded carriers (op+carrier) daily for one month. op+nec treatment reduced the pdf by suppression of fibrotic markers ... | 2016 | 26542278 |
| diagnosis of opisthorchis viverrini infection with handheld microscopy in lao people's democratic republic. | opisthorchiasis is a neglected tropical disease, yet it is of considerable public health importance in southeast asia given the predilection for chronically infected persons to develop cholangiocarcinoma. we evaluated a handheld microscope for the diagnosis of opisthorchis viverrini in a community-based setting in lao people's democratic republic in comparison with conventional light microscopy. in stool samples collected from 104 individuals, handheld microscopy revealed a sensitivity of 70.6% ... | 2016 | 26526923 |
| bithynia siamensis goniomphalos, the first intermediate host of opisthorchis viverrini in thailand. | opisthorchiasis caused by opisthorchis viverrini (o. viverrini) remains as medically important problem in thailand especially in the north-eastern part. infection with this parasite can lead to cholangiocarcinoma improvement. the highest prevalence of o. viverrini infection has been found in the northeast thailand and is associated with the high incidence rate of cholangiocarcinoma. to complete the life cycle of o. viverrini, the freshwater snails namely bithynia funiculata, bithynia siamensis s ... | 2015 | 26522291 |
| trematode infection of freshwater snail, family bithyniidae in thailand. | opisthorchis viverrini is restricted to and requires for its aquatic life cycle only bithynia snail as first intermediate host but many species of cyprinid fish as second intermediate hosts. a survey in thailand of trematode infection in freshwater snails of the family bithyniidae carried out during october 2008 - july 2009 found a total of 5,492 snails, classified into ten species distributed in various geographic areas. bithyniafuniculata and gabbia pygmaea were localized to the north, b. s. g ... | 2015 | 26521513 |
| development and validation of an enantioselective lc-ms/ms method for the analysis of the anthelmintic drug praziquantel and its main metabolite in human plasma, blood and dried blood spots. | praziquantel (pzq) is the treatment of choice against various trematode and cestode infections. to study the pharmacokinetics of pzq in patients infected with the liver fluke opisthorchis viverrini, we developed and validated an enantioselective liquid chromatography coupled to tandem mass spectrometry method for the analysis of r - and s -pzq and its r -trans-4-oh-pzq metabolite in human plasma, blood and dried blood spots (dbs). the analytes were detected in the positive mode using selected re ... | 2016 | 26517852 |
| community participation in cholangiocarcinoma prevention in ubon ratchathani, thailand: relations with age and health behavior. | a high prevalence of opisthorchis viverrini infection is usually found in wetland geographical areas of thailand where people have traditional behavior of eating uncooked freshwater fish dishes which results in cholangiocarcinoma (cca) development. there were several approaches for reducing opisthorchiasis-linked cca, but the prevalence remains high. to develop community participation as a suitable model for cca prevention is, firstly, to know what factors are related. we therefore aimed to inve ... | 2015 | 26514539 |
| review and current status of opisthorchis viverrini infection at the community level in thailand. | opisthorchis viverrini is remains a public health problem in thailand, particularly in the northeast and north regions which have the highest incidences of chonalgiocarcinoma (cca). o. viverrini causes the disease opithorchiasis, and its has been classified as a group 1 biological carcinogen. humans, dogs, and cats become infected with o. viverrini by ingesting raw or undercooked fish containing infective metacercariae. the first human cases of o. viverrini infection were reported in thailand 10 ... | 2015 | 26514452 |
| human trichostrongyliasis: a hospital case series. | trichostrongylus is a common nematode found to infect livestock throughout the tropics and can cause accidental zoonosis in humans. in the lao pdr and thailand, cases of human trichostrongyliasis have been reported sporadically but clinical data are limited. we retrospectively reviewed 41 cases of trichostrongyliasis who presented to srinagarind hospital, thailand from 2005 to 2012. the diagnosis of trichostrongyliasis was made by finding their eggs in the stool of patients. of the 41 cases revi ... | 2015 | 26513921 |
| association between praziquantel treatment and cholangiocarcinoma: a hospital-based matched case-control study. | infection by the liver fluke, opisthorchis viverrini, remains an important public health problem in thailand and has resulted in the highest prevalence of infection and incidence of subsequent cholangiocarcinoma (cca) in the world. praziquantel (pzq) is the antihelminthic drug of choice for treatment. previous studies in hamsters showed that repeated infection and pzq treatment could increase the risk of cca. however, the few available epidemiology studies in humans have shown unclear evidence o ... | 2015 | 26496745 |
| carcinogenic parasite secretes growth factor that accelerates wound healing and potentially promotes neoplasia. | infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). injury from feeding activities of this parasite within the human biliary tree causes extensive lesions, wounds that undergo protracted cycles of healing, and re-injury over years of chronic infection. we show that o. viverrini secreted proteins accelerated wound resolution in human cholangiocytes, an outcome that was compromised following silencing of expression of the fluke-de ... | 2015 | 26485648 |
| advances in the diagnosis of human opisthorchiasis: development of opisthorchis viverrini antigen detection in urine. | many strategies to control opisthorchiasis have been employed in thailand, but not in the other neighbouring countries. specific control methods include mass drug administration (mda) and health education to reduce raw fish consumption. these control efforts have greatly shifted the epidemiology of opisthorchis viverrini (ov) infection over the last decade from presenting as densely concentrated "heavy" infections in single villages to widespread "light" ov infections distributed over wide geogr ... | 2015 | 26485024 |
| the mirnaome of opisthorchis viverrini induced intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is an aggressive cancer, arising in the biliary ducts that extend into the liver. the highest incidence of icc occurs in southeast asia, particularly in the mekong river basin countries of thailand, laos, cambodia, and vietnam, where it is strongly associated with chronic infection by the food-borne liver fluke opisthorchis viverrini (ov), one of only three eukaryote pathogens considered group one carcinogens. intrahepatic cholangiocarcinoma is usually diagn ... | 2014 | 26484108 |
| urinary microrna-192 and microrna-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group. | opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (pdf), which possibly associates to cholangiocarcinoma (cca). patients with cca have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. hence, detecting early stage cca is essential. secretory mirnas have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. we aimed to determine levels of mir-192 a ... | 2015 | 26456596 |
| upregulation of 14-3-3 eta in chronic liver fluke infection is a potential diagnostic marker of cholangiocarcinoma. | to discover protein markers in chronic/advanced opisthorchiasis for the early detection of opisthorchis viverrini (ov)-associated cholangiocarcinoma (cca). | 2016 | 26435198 |
| anticancer activity of atractylodes lancea (thunb.) dc in a hamster model and application of pet-ct for early detection and monitoring progression of cholangiocarcinoma. | opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) is an important cancer in the great mekong region, particularly in thailand. limitations of treatment options and the lack of an effective diagnostic tool for early detection of cca are major concerns for the control of this type of cancer. the aim of the study was to investigate anti-cca activity of the ethanolic extract of atractylodes lancea (thunb.) dc., and the applicability of positron emission tomography-computed tomography (pet ... | 2015 | 26434829 |
| whole transcriptome profiling of adult and infective stages of the trematode opisthorchis felineus. | opisthorchis felineus, the trematode belonging to the family opisthorchiidae, is a causative agent of the infection called opisthorchiasis or liver fluke infection. being a close relative of opisthorchis viverrini and clonorchis sinensis (oriental liver flukes) it is encountered in northern eurasia, especially in russia, kazakhstan, belarus, ukraine, and baltic countries. whole genome data for oriental liver flukes revealed their adaptations for life in the bile duct but our knowledge of o. feli ... | 2016 | 26363139 |
| immunodiagnosis of opisthorchiasis using parasite cathepsin f. | opisthorchis viverrini, a food-borne trematode parasite endemic in the lower mekong countries, is conventionally diagnosed by stool examination. however, parasitological stool-based diagnosis can be unreliable in light infections. the goal of this study was to develop the immunodiagnosis of opisthorchiasis using cathepsin f cysteine protease of o. viverrini in both indirect and sandwich elisa assays. a recombinant o. viverrini cathepsin f (rov-cf) of 40 kda was expressed in e. coli strain bl21 ( ... | 2015 | 26344868 |
| pathological characteristics of intraductal polypoid neoplasms of bile ducts in thailand. | intraductal papillary or tubular neoplasms of the bile duct have recently been proposed as one of the pre-invasive lesions of cholangiocarcinoma. herein, a total of 50 cases of intraluminal polypoid neoplasms of the bile ducts experienced in khon kaen university hospital in thailand were pathologically examined. these cases presumably had a history of infection of opisthorchis viverrini. these neoplasms were histologically composed of high-grade intraepithelial neoplasm showing a tubular and/pap ... | 2015 | 26339398 |
| infection status of zoonotic trematode metacercariae in fishes from vientiane municipality and champasak province in lao pdr. | the infection status of fishborne zoonotic trematode (fzt) metacercariae was investigated in fishes from 2 localities of lao pdr. total 157 freshwater fishes (17 species) were collected in local markets of vientiane municipality and champasak province in december 2010 and july 2011, and each fish was examined by the artificial digestion method. total 6 species of fzt metacercariae, i.e., opisthorchis viverrini, haplorchis taichui, haplorchis yokogawai, haplorchis pumilio, centrocestus formosanus ... | 2015 | 26323843 |
| intestinal helminths recovered from humans in xieng khouang province, lao pdr with a particular note on haplorchis pumilio infection. | a survey of intestinal helminths was undertaken in riparian people in xieng khouang province, lao pdr. fecal specimens were collected from 643 people (289 males and 354 females) residing in 4 districts (nonghet, kham, phoukout, and pek) and were examined by the kato-katz technique. the overall helminth egg positive rate was 41.2%, and hookworms revealed the highest prevalence (32.7%) followed by trichuris trichiura (7.3%) and ascaris lumbricoides (5.6%). the positive rate for small trematode egg ... | 2015 | 26323842 |
| combination of praziquantel and aspirin minimizes liver pathology of hamster opisthorchis viverrini infection associated cholangiocarcinoma. | opisthorchiasis is one of the major risk factors for cholangiocarcinoma (cca) in northeastern thailand. an effective drug for killing this parasite is praziquantel. recently, several reports have shown that with frequent use, praziquantel may itself be a cca risk and can cause liver cell damage from an immunopathological response after parasite death. aspirin has many properties including anti-inflammation and anti-cancer. therefore, we use of aspirin (as) and praziquantel (pz) to improve hepato ... | 2016 | 26277607 |
| development and evaluation of pcr methods based on cytochrome c oxidase subunit one (cox1) and nadh dehydrogenase subunit one gene (nad1) to detect opisthorchis viverrini in human fecal samples. | opisthorchis viverrini is highly prevalent throughout southeast asia. chronic infection of this parasite leads to cholangiocarcinoma (cca), a fatal bile duct cancer. the early and accurate detection of this parasite is very important; therefore, new pcr methods targeting cytochrome c oxidase subunit one and nadh dehydrogenase subunit one gene to detect o. viverrini in fecal specimens have been developed. ninety o. viverrini-positive human fecal samples were used in this study. the pcr sensitivit ... | 2015 | 26239798 |
| levels of 8-oxodg predict hepatobiliary pathology in opisthorchis viverrini endemic settings in thailand. | opisthorchis viverrini is distinct among helminth infections as it drives a chronic inflammatory response in the intrahepatic bile duct that progresses from advanced periductal fibrosis (apf) to cholangiocarcinoma (cca). extensive research shows that oxidative stress (os) plays a critical role in the transition from chronic o. viverrini infection to cca. os also results in the excision of a modified dna lesion (8-oxodg) into urine, the levels of which can be detected by immunoassay. herein, we m ... | 2015 | 26230769 |
| data set from the proteomic analysis of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini. | the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. this data article contains the results obtained from the analysis of the proteins differentially expressed in the snail b. siamensis goniomphalos upon infection with o. viverrini. it contains the data generated from iquantitator software including a pdf of each sample with a protein׳s relative expression ... | 2015 | 26217698 |
| risk areas of liver flukes in surin province of thailand using geographic information system. | opisthorchiasis, caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao pdr and thailand. | 2015 | 26201130 |
| localization of tubulin from the carcinogenic human liver fluke, opisthorchis viverrini. | opisthorchiasis caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao people's democratic republic and thailand. the infection is associated with a number ofhepatobiliary diseases including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, cholelithiasis and cholangiocarcinoma. | 2015 | 26201128 |
| excretory/secretory products of the carcinogenic liver fluke are endocytosed by human cholangiocytes and drive cell proliferation and il6 production. | liver fluke infection caused by opisthorchis viverrini remains a major public health problem in many parts of asia including thailand, lao pdr, vietnam and cambodia, where there is a strikingly high incidence of cholangiocarcinoma (cca - hepatic cancer of the bile duct epithelium). among other factors, uptake of o. viverrini excretory/secretory products (oves) by biliary epithelial cells has been postulated to be responsible for chronic inflammation and proliferation of cholangiocytes, but the m ... | 2015 | 26187786 |
| mri and (1)h mrs findings of hepatobilary changes and cholangiocarcinoma development in hamsters infected with opisthorchis viverrini and treated with n-nitrosodimethylamine. | 3 t mri and (1)h mrs were useful for quantitative investigation of the serial development of hepatobiliary changes in opisthorchis viverrini infection in hamsters, and the differential diagnosis of cholangiocacinoma (cca) development from bile duct changes and normal condition is unclear. in this study, we investigated the serial development of hepatobiliary changes and ccagenesis in o. viverrini-infected and n-nitrosodimethylamine (ndma) treated hamsters (on group) using 3 t mri and (1)h mrs an ... | 2015 | 26117689 |
| [a 55-year old thai male patient with acute right upper quadrant abdominal pain]. | we report on a 55-year-old thai male who presented during a visit to germany with acute abdominal pain in the right upper quadrant and signs of cholestasis. the diagnosis of liver fluke infection (opisthorchiasis) was confirmed by the detection of the characteristic eggs of opisthorchis viverrini in stool microscopy. the patient was treated with praziquantel. opisthorchiasis is a common parasitic disease and major public health problem in southeast asia and in eastern europe. | 2015 | 26116106 |
| risk factors for opisthorchis viverrini infection in nong khai province, thailand. | opisthorchis viverrini (ov) infection is the main risk factor for cholangiocarcinoma and is often found in northeastern thailand. the prevalence of ov infection and the incidence of cholangiocarcinoma are major public health problems in this region. | 2015 | 26107209 |
| lectin microarray-based sero-biomarker verification targeting aberrant o-linked glycosylation on mucin 1. | glycoform of mucin 1 (muc1) in cancerous cells changes markedly with cell differentiation, and thus, qualitative detection and verification of the muc1 glycosylation changes have potential diagnostic value. we have developed an ultrasensitive method to detect the changes in cholangiocarcinoma (cc), which produces muc1, and applied it in the diagnostics development. the focused glycan analysis using 43-lectin-immobilized microarray could obtain the glycan profiles of sialylated muc1 in 5 μl of se ... | 2015 | 26091356 |
| prevalence and risk factors for infection by opisthorchis viverrini in an urban area of mahasarakham province, northeast thailand. | the aim of this cross-sectional analytic study was to investigate the prevalence and risk factors for opisthorchis viverrini (ov) infection in an urban area of northeastern thailand. the participants were 254 household representatives aged 15 years or older living in the most urbanised part of chiang yuen municipality in mahasarakham province. all participants provided stool samples which were examined using the modified kato-katz procedure, and a structured interview questionnaire was used to c ... | 2015 | 26028068 |
| [role of opisthorchis felineus on induction of bile duct cancer]. | opisthorchis felineus (trematoda) is widespread in the russian federation, especially in siberia, and other countries of europe. infestation of endemic area population with o. felineus reaches 80%. on animal models of the infection of closely related opisthorchis viverrini combined with the nitrosamines' intake it has been shown that the parasite induces cholangiocarcinoma. however carcinogenic potential of o. felineus is still poorly studied. the present study is aimed to investigate the role o ... | 2015 | 26016330 |
| apoptosis of cholangiocytes modulated by thioredoxin of carcinogenic liver fluke. | chronic infection with the food-borne liver fluke, opisthorchis viverrini, frequently induces cancer of the bile ducts, cholangiocarcinoma. opisthorchiasis is endemic in thailand, lao pdr, cambodia and vietnam, where eating undercooked freshwater fish carrying the juvenile stage of this pathogen leads to human infection. because inhibition of apoptosis facilitates carcinogenesis, this study investigated modulation by thioredoxin from o. viverrini of apoptosis of bile duct epithelial cells, chola ... | 2015 | 26007234 |
| trematode diversity in the freshwater snail bithynia siamensis goniomphalos sensu lato from thailand and lao pdr. | in order to obtain a comprehensive understanding of trematode diversity in bithynia siamensis goniomphalos sensu lato, the first intermediate host of the liver fluke opisthorchis viverrini s.l., the prevalence of larval trematode species was investigated in different localities in thailand and lao people's democratic republic (lao pdr). in thailand, snail samples were collected from 29 localities in the nine provinces: buri ram, surin, chaiya phum, maha sarakham, khon kaen, kalasin, mukdahan, sa ... | 2016 | 25997613 |
| carcinogenic liver fluke secretes extracellular vesicles that promote cholangiocytes to adopt a tumorigenic phenotype. | throughout asia, there is an unprecedented link between cholangiocarcinoma and infection with the liver fluke opisthorchis viverrini. multiple processes, including chronic inflammation and secretion of parasite proteins into the biliary epithelium, drive infection toward cancer. until now, the mechanism and effects of parasite protein entry into cholangiocytes was unknown. | 2015 | 25985904 |
| re-examination of opisthorchis viverrini infection in northeast thailand. | liver fluke infection caused by the parasite opisthorchis viverrini (o. viverrini), a human carcinogen, is endemic in north-eastern thailand and remains a major health problem. | 2015 | 25921154 |
| seasonal transmission of opisthorchis viverrini sensu lato and a lecithodendriid trematode species in bithynia siamensis goniomphalos snails in northeast thailand. | seasonal changes play roles in the transmission success of fish-borne zoonotic trematodes (fzt). this study examined the seasonal transmission patterns of opisthorchis viverrini sensu lato (s.l.) and a virgulate cercaria (family lecithodendriidae) in the snail intermediate host, bithynia siamensis goniomphalos in northeast thailand. snail samples were collected monthly during the rainy, cool, and hot seasons during 2012-2013 to determine the prevalence and intensity of larval trematode infection ... | 2015 | 25918210 |
| diabetes mellitus: possible risk and promoting factors of cholangiocarcinoma: association of diabetes mellitus and cholangiocarcinoma. | the highest incidence of cholangiocarcinoma (cca), a malignancy of bile duct epithelia, is in the northeast of thailand. the liver fluke, opisthorchis viverrini, is the known risk factor for cca development in this region. approximately 1% of o. viverrini infected individuals develop cca. there could be other factors that influence the cholangiocarcinogenesis particularly in the o. viverrini infected individuals. the global epidemiological studies of risk factors for cca in non-o. viverrini rela ... | 2015 | 25910864 |
| a microrna profile associated with opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma. | intrahepatic cholangiocarcinoma (icc) is a highly aggressive tumor of the bile duct, and a significant public health problem in east asia, where it is associated with infection by the parasite opisthorchis viverrini. icc is often detected at an advanced stage and with a poor prognosis, making a biomarker for early detection a priority. | 2015 | 25903557 |
| comparative evaluation of strongyloides ratti and s. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis. | the use of strongyloides ratti as heterologous antigen for serodiagnosis of strongyloidiasis is preferable to strongyloides from humans due to the ease and safety of antigen preparation. in southeast asia where opisthorchis viverrini coexists with strongyloides stercoralis, there has been no report in using s. ratti for serodiagnosis of s. stercoralis. in this study, performance of an enzyme-linked immunosorbent assay (elisa) based on s. ratti was compared with that based on s. stercoralis for d ... | 2015 | 25877389 |
| synergistic effects of isomorellin and forbesione with doxorubicin on apoptosis induction in human cholangiocarcinoma cell lines. | chemotherapy for advanced cholangiocarcinoma (cca) is largely ineffective, but innovative combinations of chemotherapeutic agents and natural compounds represent a promising strategy. in our previous studies, isomorellin and forbesione, caged xanthones isolated from garcinia hanburyi, were found to induce cell cycle arrest and apoptosis in cca cell lines. the subject of our inquiry is the synergistic effect(s) of these caged xanthones with doxorubicin on growth inhibition and apoptosis induction ... | 2014 | 25866479 |
| quantitative changes in tumor-associated m2 macrophages characterize cholangiocarcinoma and their association with metastasis. | the tumor microenvironment (tme) includes numerous non-neoplastic cells such as leukocytes and fibroblasts that surround the neoplasm and influence its growth. tumor-associated macrophages (tams) and cancer-associated fibroblasts (cafs) are documented as key players in facilitating cancer appearance and progression. alteration of the macrophage (cd68, cd163) and fibroblast (α-sma, fsp-1) cells in opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) was here assessed using liver tissues f ... | 2015 | 25854403 |
| anthelmintic, anti-inflammatory and antioxidant effects of garcinia mangostana extract in hamster opisthorchiasis. | administration of praziquantel for treatment of liver fluke infection may affect the host, with mild and severe effects after treatment caused by host immune response. therefore, we focused on the antioxidant property, inflammatory and anthelmintic effects of the traditional folk medicine, g. mangostana pericarp extract, in hamster opisthorchiasis. syrian hamsters were divided into four groups: normal (control) (n); administered g. mangostana alone (gm); infected with opisthorchis viverrini alon ... | 2015 | 25836376 |
| gis-based spatial statistical analysis of risk areas for liver flukes in surin province of thailand. | it is urgently necessary to be aware of the distribution and risk areas of liver fluke, opisthorchis viverrini, for proper allocation of prevention and control measures. this study aimed to investigate the human behavior, and environmental factors influencing the distribution in surin province of thailand, and to build a model using stepwise multiple regression analysis with a geographic information system (gis) on environment and climate data. the relationship between the human behavior, attitu ... | 2015 | 25824758 |
| plasma igg autoantibody against actin-related protein 3 in liver fluke opisthorchis viverrini infection. | opisthorchiasis secondary to opisthorchis viverrini infection leads to cholangiocellular carcinoma through chronic inflammation of the bile ducts and possibly inducing autoimmunity. it was hypothesized that plasma autoantibodies directed against self-proteins are biomarkers for opisthorchiasis. plasma from patients with opisthorchiasis was tested using proteins derived from immortalized cholangiocyte cell lines, and spots reacting with plasma were excised and subjected to lc-ms/ms. seven protein ... | 2015 | 25809205 |
| genome-wide characterization of microsatellites and marker development in the carcinogenic liver fluke clonorchis sinensis. | clonorchis sinensis is an important carcinogenic human liver fluke endemic in east and southeast asia. there are several conventional molecular markers that have been used for identification and genetic diversity; however, no information about microsatellites of this liver fluke is published so far. we here report microsatellite characterization and marker development for a genetic diversity study in c. sinensis, using a genome-wide bioinformatics approach. based on our search criteria, a total ... | 2015 | 25782682 |
| the carcinogenic liver fluke opisthorchis viverrini is a reservoir for species of helicobacter. | there has been a strong, positive correlation between opisthorchiasis-associated cholangiocarcinoma and infection with helicobacter. here a rodent model of human infection with opisthorchis viverrini was utilized to further investigate relationships of apparent co-infections with o. viverrini and h. pylori. a total of 150 hamsters were assigned to five groups: i) control hamsters not infected with o. viverrini; ii) o. viverrini-infected hamsters; iii) non-o. viverrini infected hamsters treated w ... | 2015 | 25773821 |
| plasma phosphoproteome and differential plasma phosphoproteins with opisthorchis viverrini-related cholangiocarcinoma. | this study was conducted to investigate the plasma phosphoproteome and differential plasma phosphoproteins in cases of of opisthorchis viverrini (ov)-related cholangiocarcinoma (cca). plasma phosphoproteomes from cca patients (10) and non-cca subjects (5 each for healthy subjects and ov infection) were investigated using gel-based and solution-based lc-ms/ms. phosphoproteins in plasma samples were enriched and analyzed by lc-ms/ms. strap, panther, ipath, and mev programs were applied for the ide ... | 2015 | 25735322 |
| protection against fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin l. | fasciola gigantica cathepsin l1h (fgcatl1h) is one of the major cathepsin l released by juveniles of f. gigantica to aid in the invasion of host's tissues. due to its high sequence similarity with other cathepsin l (catl) isoforms of late stage f. gigantica, it was considered to be a good vaccine candidate that can block all catl-mediated protease activities and affect juveniles as well as adult parasites. in this study, recombinant profgcatl1h protein expressed in yeast, pichia pastoris, system ... | 2015 | 25701317 |
| genetics of opisthorchis viverrini-related cholangiocarcinoma. | we review the genetic, epigenetic and transcriptional landscape of liver fluke (opisthorchis viverrini, ov)-related cholangiocarcinoma (cca). its distinct alterations, as compared with non-ov-related cca may help shed light on its underlying molecular mechanisms. | 2015 | 25693006 |
| bile canalicular changes and defective bile secretion in opisthorchis viverrini-infected hamsters. | infection with the liver fluke opisthorchis viverrini (digenea) (poirier, 1886) causes bile duct injury and periductal fibrosis by chronic overproduction of inflammatory-mediators and eventually results in cholangiocarcinoma development. while extensive research works have been done on o. viverrini infection-associated changes of bile ducts and periductal fibrosis, little attention was paid on morphological and biochemical changes of the bile canaliculi (bc), the origin of bile flow. we aimed to ... | 2014 | 25651692 |
| schistosome and liver fluke derived catechol-estrogens and helminth associated cancers. | infection with helminth parasites remains a persistent public health problem in developing countries. three of these pathogens, the liver flukes clonorchis sinensis, opisthorchis viverrini and the blood fluke schistosoma haematobium, are of particular concern due to their classification as group 1 carcinogens: infection with these worms is carcinogenic. using liquid chromatography-mass spectrometry (lc-ms/ms) approaches, we identified steroid hormone like (e.g., oxysterol-like, catechol estrogen ... | 2014 | 25566326 |
| genome mining offers a new starting point for parasitology research. | parasites including helminthes, protozoa, and medical arthropod vectors are a major cause of global infectious diseases, affecting one-sixth of the world's population, which are responsible for enormous levels of morbidity and mortality important and remain impediments to economic development especially in tropical countries. prevalent drug resistance, lack of highly effective and practical vaccines, as well as specific and sensitive diagnostic markers are proving to be challenging problems in p ... | 2015 | 25563615 |
| opisthorchis viverrini infection activates the pi3k/ akt/pten and wnt/β-catenin signaling pathways in a cholangiocarcinogenesis model. | opisthorchis viverrini (ov) infection is the major etiological factor for cholangiocarcinoma (cca), especially in northeast thailand. we have previously reported significant involvement of pi3k/akt/pten and wnt/β- catenin in human cca tissues. the present study, therefore, examined the expression and activation of pi3k/ akt/pten and wnt/β-catenin signaling components during ov-induced cholangiocarcinogenesis in a hamster animal model. hamsters were divided into two groups; non-treated and ov plu ... | 2014 | 25556493 |
| lc-ms/ms method for the determination of two metabolites of tribendimidine, deacylated amidantel and its acetylated metabolite in plasma, blood and dried blood spots. | tribendimidine has emerged as potential alternative to praziquantel in the treatment of opisthorchis viverrini infections. to support its clinical development program, a quantitative high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) assay was developed for tribendimidine's degradation product deacylated amidantel (dadt) and its acetylated metabolite adadt. analytical sample preparation included protein precipitation for blood and plasma, and direct processing of dried ... | 2015 | 25553533 |
| [the reagents kit to detect metyorchis biis, opisthorchis viverrini and clonorchis sinensis, opisthorchis felineus--agents of opisthorchiasis using technique of polymerase chain reaction in real-time]. | the helminths opisthorchis felineus, opisthorchis viverrini, clonorchis sinensis, metorchis bilis are the agents of opisthorchiasis. the actual diagnostic of parasitic diseases based on microscope analysis of samples of human feces to detect presence of ova of parasites suffers of many shortcomings, in particular low sensitivity especially at earlier stages. the purpose of this study was to compare results of detection of parasites using both classical technique and technique of specific differe ... | 2014 | 25552048 |
| chronic opisthorchis viverrini infection and associated hepatobiliary disease is associated with iron loaded m2-like macrophages. | chronic opisthorchis viverrini-induced hepatobiliary disease is associated with significant leukocyte infiltration, including activated macrophages; however, the polarization of infiltrating macrophages remains to be fully characterized. in this study, we characterized macrophage polarization and phenotype in chronic o. viverrini-induced hepatobiliary disease in humans and hamsters using gene expression and histochemical analysis. chronic o. viverrini infection and associated hepatobiliary disea ... | 2014 | 25548425 |
| prevalence of intestinal helminths among inhabitants of cambodia (2006-2011). | in order to investigate the status of intestinal helminthic infections in cambodia, epidemiological surveys were carried out on a national scale, including 19 provinces. a total of 32,201 fecal samples were collected from schoolchildren and adults between 2006 and 2011 and examined once by the kato-katz thick smear technique. the overall egg positive rate of intestinal helminths was 26.2%. the prevalence of hookworms was the highest (9.6%), followed by that of opisthorchis viverrini/minute intes ... | 2014 | 25548418 |
| infection and cancer: global distribution and burden of diseases. | infection is one of the main risk factors for cancer. | 2014 | 25512154 |
| bi-functionality of opisthorchis viverrini aquaporins. | aquaporins (aqp) are essential mediators of water regulation in all living organisms and members of the major intrinsic protein (mip) superfamily of integral membrane proteins. they are potential vehicles or targets for chemotherapy, e.g. in trypanosoma brucei melarsoprol and pentamidine uptake is facilitated by tbaqp-2. transcriptome data suggests that there are at least three active aquaporins in the human liver fluke, opisthorchis viverrini, ovaqp-1, 2 and 3, and crude rna silencing of ovaqp- ... | 2015 | 25461277 |
| suppression of ov-grn-1 encoding granulin of opisthorchis viverrini inhibits proliferation of biliary epithelial cells. | multistep processes likely underlie cholangiocarcinogenesis induced by chronic infection with the fish-borne liver fluke, opisthorchis viverrini. one process appears to be cellular proliferation of the host bile duct epithelia driven by excretory-secretory (es) products of this pathogen. specifically, the secreted growth factor ov-grn-1, a liver fluke granulin, is a prominent component of es and a known driver of hyper-proliferation of cultured human and mouse cells in vitro. we show potent hype ... | 2015 | 25450776 |
| anti-inflammatory and anti-periductal fibrosis effects of an anthocyanin complex in opisthorchis viverrini-infected hamsters. | the pharmacological activities of herbal extracts can be enhanced by complex formation. in this study, we manipulated cyanidin and delphinidin-rich extracts to form an anthocyanin complex (ac) with turmeric and evaluated activity against inflammation and periductal fibrosis in opisthorchis viverrini-infected hamsters. the ac was prepared from anthocyanins extracted from cobs of purple waxy corn (70%), petals of blue butterfly pea (20%) and turmeric extract (10%), resulting in an enhanced free-ra ... | 2014 | 25447758 |
| reprint of "experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus". | opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24 mm) of barbonymus gonionotus fish on infection success of o. vive ... | 2015 | 25446173 |
| seasonal cercarial emergence patterns of opisthorchis viverrini infecting bithynia siamensis goniomphalos from vientiane province, lao pdr. | snail intermediate hosts play a pivotal role in maintaining the life cycles of trematodes, including opisthorchis viverrini. we investigated the emergence patterns of o. viverrini cercariae infecting bithynia siamensis goniomphalos at foci in an endemic area in vientiane province, lao pdr. | 2014 | 25442515 |
| traditional herbal medicine for the control of tropical diseases. | throughout history, traditional herbal medicine has afforded a rich repository of remedies with diverse chemical structures and bioactivities against several health disorders. a common issue of herbal medicine is the limitation of information on their pharmacological activities and their active constituents. traditionally, the use of herbal medicine has been based on empirical treatment and passed on from generation to generation with information available only in local journals. this prevents s ... | 2014 | 25425945 |
| selective immunostaining of mehlis' gland of opisthorchis viverrini by antibody against rat diacylglycerol kinase. | the mehlis' gland of opisthorchis viverrini was selectively and intensely immunopositive with an antibody against rat diacylglycerol kinase gamma, and its entire structure with associated radiating processes was clearly demonstrated by immuno-light microscopy. in immuno-electron microscopy, the immunopositive processes were revealed to contain many vesicles and vacuoles and the immunoreactive materials were deposited diffusely in the cytoplasm except for the vesicular interior. the present findi ... | 2014 | 25417499 |
| genetic and environmental determinants of risk for cholangiocarcinoma in thailand. | cholangiocarcinoma (cca) is a difficult cancer to diagnose in the early stage and to treat by curative resection. the incidence of cca in the northeast of thailand is the highest in the world. to make progress in detecting a high risk group and in the prevention and detection of cca, we have been analyzing the risk factors for cca. although liver fluke infection is known to be a risk factor, there are patients who are not infected with the liver fluke and not all people infected with the liver f ... | 2014 | 25401000 |
| rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini using real-time pcr and high resolution melting analysis. | clonorchis sinensis and opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in asia. the present study developed an assay integrating real-time pcr and high resolution melting (hrm) analysis for the specific detection and rapid identification of c. sinensis and o. viverrini. primers targeting cox1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes. assays using genomic dna ex ... | 2014 | 25386621 |
| gene-environment interaction involved in cholangiocarcinoma in the thai population: polymorphisms of dna repair genes, smoking and use of alcohol. | cholangiocarcinoma (cca) is the most common malignancy in a northeast thai population. smoking and alcohol drinking are associated with the production of free radical intermediates, which can cause several types of dna lesions. reduced repair of these dna lesions would constitute an important risk factor for cancer development. we therefore examined whether polymorphisms in dna base-excision repair (ber) genes, xrcc1 g399a and ogg1 c326g, were associated with cca risk and whether they modified t ... | 2014 | 25335960 |
| immunization and challenge shown by hamsters infected with opisthorchis viverrini following exposure to gamma-irradiated metacercariae of this carcinogenic liver fluke. | here we report findings to optimize and standardize conditions to attenuate metacercariae of opisthorchis viverrini by ionizing radiation to elicit protective immune responses to challenge infection. metacercariae were gamma-irradiated and the ability of irradiated metacercariae to prevent patent infection of challenge metacercariae in hamsters was determined, as well as their ability to induce a host antibody response. metacercariae irradiated in a dose-dependent manner, with 3, 5, 10, 12, 20, ... | 2016 | 25315797 |
| multiparasitism and intensity of helminth infections in relation to symptoms and nutritional status among children: a cross-sectional study in southern lao people's democratic republic. | the occurrence and spatial distribution of intestinal helminth infection in children is fairly well understood. however, knowledge on how helminth infections govern intestinal morbidity is scarce. we conducted a cross-sectional study to assess and quantify the relationship between single and multiple species helminth infection with clinical and self-reported morbidity indicators and nutritional status in champasack province, southern lao people's democratic republic (lao pdr). a random sample of ... | 2015 | 25291046 |
| epidemiology of strongyloides stercoralis on mekong islands in southern laos. | strongyloides stercoralis is a neglected helminth infection potentially that can lead to systemic infection in immunocompromised individuals. in lao people's democratic republic (lao pdr, laos), information on s. stercoralis infection is scarce. we assessed s. stercoralis infection and associated risk factors and symptoms on the mekong islands in southern laos. baermann and kato-katz techniques were performed on two stool samples from each individual to detect s. stercoralis larvae and concomita ... | 2015 | 25291044 |
| proteomic profile of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini. | the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. the undisputed link between cca and o. viverrini infection has precipitated efforts to understand the molecular basis of host-parasite interactions with a view to ultimately developing new control strategies to combat this carcinogenic infection. to date most effort has focused on the interactions betwee ... | 2015 | 25284051 |
| subtle to severe hepatobiliary morbidity in opisthorchis viverrini endemic settings in southern laos. | evidence of severe hepatobiliary morbidity associated with opisthorchis viverrini liver fluke infection including cholangiocarcinoma (cca) is scarce in laos although o. viverrini infection is highly prevalent. we assessed hepatobiliary morbidity using abdominal ultrasonography (us) in o. viverrini adult patients in saravan province, southern laos. a random sample of 431 o. viverrini patients from 10 villages underwent abdominal us. mild, moderate and markedly advanced periductal fibrosis was dia ... | 2015 | 25275346 |
| specific diagnosis of opisthorchis viverrini using loop-mediated isothermal amplification (lamp) targeting parasite microsatellites. | opisthorchis viverrini and other food-borne trematode infections are major health problems in thailand, the lao people's democratic republic, vietnam and cambodia. differential diagnosis of o. viverrini based on the microscopic observation of parasite eggs is difficult in areas where clonorchis sinensis and minute intestinal flukes coexist. recently, loop-mediated isothermal amplification (lamp) has been widely used for detection and identification of trematode for its simple method that is usef ... | 2015 | 25268466 |
| repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: a cross-sectional survey in southern lao people's democratic republic. | intestinal parasitic infections are common in lao people's democratic republic (lao pdr). we investigated the accuracy of the kato-katz (kk) technique in relation to varying stool sampling efforts, and determined the effect of the concurrent use of a quantitative formalin-ethyl acetate concentration technique (fect) for helminth diagnosis and appraisal of concomitant infections. the study was carried out between march and may 2006 in champasack province, southern lao pdr. overall, 485 individual ... | 2015 | 25225157 |
| prevalence and risk factors for opisthorchis viverrini infections in upper northeast thailand. | opisthorchis viverrini is an ongoing public health problem in northeast thailand. despite continuous efforts for decades by healthcare organizations to overcome this problem, infection rates remain high. to enable related personnel to identify and address the various issues effectively, a cross-sectional study was performed to investigate prevalence and risk factors for opisthorchiasis. the target group was 3,916 thai residents of northeast thailand who were 15 or over. participants were recruit ... | 2014 | 25169496 |
| circulating mir-192 in liver fluke-associated cholangiocarcinoma patients: a prospective prognostic indicator. | this study aimed to investigate the mir-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (cca) for a prospective prognostic indicator. | 2014 | 25131257 |
| opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like. | concurrent deficiency of rhophilin associated tail protein (ropn1) and ropn1-like (ropn1l) in mice causes structural abnormalities and immotility of sperm and thereby infertility. in the present research, ropn1l of the human liver fluke opisthorchis viverrini was molecularly characterized and showed unexpected potential as a diagnostic tool. ropn1l transcripts were detected in 2-week-old juveniles by rt-pcr. immunohistochemical analysis of the adult worm localized the protein in testis lobes, se ... | 2014 | 25124046 |
| toward integrated opisthorchiasis control in northeast thailand: the lawa project. | human liver fluke, opisthorchis viverrini, a food-borne trematode is a significant public health problem in southeast asia, particularly in thailand. despite a long history of control programmes in thailand and a nationwide reduction, o. viverrini infection prevalence remains high in the northeastern provinces. therefore, a new strategy for controlling the liver fluke infection using the ecohealth/one health approach was introduced into the lawa lake area in khon kaen province where the liver fl ... | 2015 | 25102053 |