Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [analysis of characteristics of bacteria in respiratory tract infection in 2013-2016 in heibei 3a hospital: a single-center report of 7 497 patients]. | to analyze the changes and characteristics of respiratory tract bacteria in hebei 3a hospital, and to provide new rationale for clinical diagnosis and treatment. | 2017 | 28936955 |
| vetiver essential oil in cosmetics: what is new? | background:vetiver is a key ingredient for the perfume industry nowadays. however, with the constant and rapid changes of personal tastes, this appeal could vanish and this sector could decline quite quickly. new dissemination paths need to be found to tap this valuable resource.methods:in this way, its potential use in cosmetics either as an active ingredient per se (with cosmeceutical significance or presenting antimicrobial activity) has hence been explored in vitro.results:in this contributi ... | 2017 | 28930256 |
| high glucose-mediated overexpression of icam-1 in human vaginal epithelial cells increases adhesion of candida albicans. | to investigate the involvement of icam-1 in the adhesion of candida to the genitourinary epithelial cells in high glucose, we examined the adhesion of candida albicans or candida glabrata to human vaginal epithelial cells (vk2/e6e7) or human vulvovaginal epidermal cells (a431). these cells were cultured in 100, 500 or 3000 mg/dl glucose for three days and inoculated with candida for 60 minutes. followed by, adhering of candida to the cells, which were counted. while the adhesion of candida albic ... | 2018 | 28920516 |
| multidrug-resistant candida: epidemiology, molecular mechanisms, and treatment. | invasive candida infections remain an important cause of morbidity and mortality, especially in hospitalized and immunocompromised or critically ill patients. a limited number of antifungal agents from only a few drug classes are available to treat patients with these serious infections. resistance can be either intrinsic or acquired. resistance mechanisms are not exchanged between candida; thus, acquired resistance either emerges in response to an antifungal selection pressure in the individual ... | 2017 | 28911043 |
| isolation ofcandidaspecies from gastroesophageal lesions among pediatrics in isfahan, iran: identification and antifungal susceptibility testing of clinical isolates by e-test. | candidaspecies can become opportunistic pathogens causing local or systemic invasive infections. gastroesophageal candidiasis may depend on thecandidacolonization and local damage of the mucosal barrier. risk factors are gastric acid suppression, diabetes mellitus, chronic debilitating states such as carcinomas, and the use of systemic antibiotics and corticosteroids. the aim of this study is collection and molecular identification ofcandidaspecies from gastroesophageal lesions among pediatrics ... | 2017 | 28904931 |
| quantification of cdr1 gene expression in fluconazole resistant candida glabrata strains using real-time pcr. | the opportunistic fungi, particularlycandida glabratahas been known as main etiologic agents of life-threating infections in some patients. although fluconazole is the most effective antifungal agent against candidiasis,c. glabrata, fluconazole-resistant strains have been increased recently overexpression or mutations of atp-binding cassette (abc) transporter family membrane proteins such as; cg cdr1, cg cdr2 are responsible for fluconazole resistance in a large proportion of candidiasis cases. ... | 2017 | 28894714 |
| oral candidal carriage in subjects with pure vegetarian and mixed dietary habits. | candida albicansbeing a part of the normal oral microbial flora is one of the most commonly isolated species from the oral cavity. recent studies have shown a steady rise in the number of nonc. albicansspecies, which are relatively resistant to common antifungal agents and are being recognized as potential pathogens. it is vital to ascertain the predisposing factors leading to such a shift in the oral candidal flora. | 2017 | 28893036 |
| antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. | fatty acid methyl esters (fames) were obtained from vegetable oils of soybean, corn and sunflower. the current study was focused on evaluating the antifungal activity of fames mainly against paracoccidioides spp., as well as testing the interaction of these compounds with commercial antifungal drugs and also their antioxidant potential. fames presented small ic50 values (1.86-9.42 μg/ml). all three fames tested showed antifungal activity against isolates of paracoccidioides spp. with mic values ... | 2018 | 28876392 |
| microbial epidemiology of candidaemia in neonatal and paediatric intensive care units at the children's medical center, tehran. | invasive candidiasis is a major cause of morbidity and mortality in children. however, limited data are available on the epidemiology of this infection in paediatric settings in iran. the aim of this study was to determine the prevalence, microbial epidemiology, risk factors and clinical outcomes associated with candidaemia in intensive care units at the children's medical center, tehran, iran. all blood and other normally sterile specimen cultures positive for candida species were included. iso ... | 2018 | 28872714 |
| antifungal effect of cow's urine distillate on candida species. | increase in resistance of candida species, to routinely used antifungal agents has necessitated the quest for new drugs. few studies have revealed that cow's urine can suppress the growth of pathogenic fungi. however there is no published report on antifungal effects of cow's urine on clinical candida isolates. | 2018 | 28869083 |
| insights into the molecular basis for substrate binding and specificity of the fungal cystine transporter cgcyn1. | cystine transporters are a clinically important class of transporters found in bacteria, pathogenic fungi and mammalian cells. despite their significance, very little is known about the mechanism of substrate recognition and transport. we have carried out studies on the plasma membrane candida glabrata cystine transporter, cgcyn1 a member of the amino acid-polyamine-organocation (apc) transporter superfamily. a homology model of cgcyn1 was generated by using crystal structures of three known bac ... | 2017 | 28865795 |
| enantioselective bioreduction of benzo-fused cyclic ketones with engineered candida glabrata ketoreductase 1 - a promising synthetic route to ladostigil (tv3326). | biocatalysis has been recently emerging as a promising alternative to traditional chemical synthesis because of its "green" characteristics and comparable selectivities, which accord with the concept of sustainable development and demand for asymmetric synthesis. in this study, whole-cell biocatalysts containing glucose dehydrogenase (gdh) and candida glabrata ketoreductase 1 (cgkr1) variants were constructed. these biocatalysts were applied to the reduction of benzo-fused cyclic ketones and sho ... | 2017 | 28848953 |
| isocoumarin derivatives from the endophytic fungus, pestalotiopsis sp. | five new isocoumarin derivatives, pestalactone a-c (1-3) and pestapyrone d-e (4-5), together with two known compounds (6-7) were isolated from the solid cultures of the endophytic fungus pestalotiopsis sp. obtained from photinia frasery. their structures were mainly determined by extensive spectroscopic analysis, mo2(ococh3)4-induced electronic circular dichroism (ecd), and ecd calculation. compounds 1 and 2 were rare isocoumarin derivatives and derived from distinctive polyketide pathways. comp ... | 2017 | 28842359 |
| epidemiology and antifungal susceptibilities of yeast isolates causing invasive infections across urban beijing, china. | to investigate the species distribution and antifungal susceptibility profiles of yeast isolates causing invasive infections across beijing. | 2017 | 28836465 |
| stratum corneum lipid liposome-encapsulated panomycocin: preparation, characterization, and the determination of antimycotic efficacy against candida spp. isolated from patients with vulvovaginitis in an in vitro human vaginal epithelium tissue model. | in this study, a liposomal lyophilized powder formulation of panomycocin was developed for therapeutic purposes against vulvovaginal candidiasis which affects 80% of women worldwide. panomycocin is a potent antimycotic protein secreted by the yeast wickerhamomyces anomalus ncyc 434. this study involved the preparation of panomycocin-loaded stratum corneum lipid liposomes (sclls), characterization of the sclls, and determination of antimycotic efficacy of the formulation against candida albicans ... | 2017 | 28831255 |
| potential use of maldi-tof mass spectrometry for rapid detection of antifungal resistance in the human pathogen candida glabrata. | the echinocandins are relatively new antifungal drugs that represent, together with the older azoles, the recommended and/or preferred agents to treat candidaemia and other forms of invasive candidiasis in human patients. if "time is of the essence" to reduce the mortality for these infections, the administration of appropriate antifungal therapy could be accelerated by the timely reporting of laboratory test results. in this study, we attempted to validate a maldi-tof mass spectrometry-based as ... | 2017 | 28831086 |
| antifungal activity of scy-078 and standard antifungal agents against 178 clinical isolates of resistant and susceptible candida species. | scy-078in vitroactivity was determined for 178 isolates of resistant or susceptiblecandida albicans,candida dubliniensis,candida glabrata,candida krusei,candida lusitaniae, andcandida parapsilosis, including 44candidaisolates with known genotypic (fks1orfks2mutations), phenotypic, or clinical resistance to echinocandins. results were compared to those for anidulafungin, caspofungin, micafungin, fluconazole, and voriconazole. scy-078 was shown to have excellent activity against both wild-type iso ... | 2017 | 28827419 |
| comparison of genome engineering using the crispr-cas9 system in c. glabrata wild-type and lig4 strains. | candida glabrata is reported as the second most prevalent human opportunistic fungal pathogen in north america and is threatening patients all over the world. its incidence is rising, while it has developed resistance to the most widely used antifungal drugs, necessitating new approaches based on better insight into the biology of the organism. despite its close phylogenetic relationship with saccharomyces cerevisiae, generating precise genomic alterations in this species is problematic. previou ... | 2017 | 28822858 |
| differentiation of candida albicans, candida glabrata, and candida krusei by ft-ir and chemometrics by chromagar™ candida. | pathogenic candida species are detected in clinical infections. chromagar™ is a phenotypical method used to identify candida species, although it has limitations, which indicates the need for more sensitive and specific techniques. infrared spectroscopy (ft-ir) is an analytical vibrational technique used to identify patterns of metabolic fingerprint of biological matrixes, particularly whole microbial cell systems as candida sp. in association of classificatory chemometrics algorithms. on the ot ... | 2017 | 28822772 |
| (1→3)-β-d-glucan assay in monitoring response to anti-fungal therapy in fungal endocarditis. | a case is reported of candida glabrata infective endocarditis (ie) treated without surgical intervention. the study aim was to: (i) briefly discuss the outcomes of other documented cases of fungal ie managed medically with fluconazole; (ii) discuss the (1→3)-β-d-glucan assay and its previously studied role in the diagnosis of invasive fungal infections; and (iii) examine a possible application of the (1→3)-β-d-glucan assay to monitor response to antifungal treatment in patients with candida endo ... | 2017 | 28820552 |
| voriconazole antifungal prophylaxis in children with malignancies: a nationwide study. | antifungal prophylaxis (afp) is recommended in at-risk hematology-oncology patients. we evaluated the safety of afp with voriconazole (vrc) in pediatric hematology/oncology patients. | 2017 | 28816795 |
| relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in brazilian clinical strains of candida glabrata. | candida glabrata is a facultative intracellular opportunistic fungal pathogen in human infections. several virulence-associated attributes are involved in its pathogenesis, host-pathogen interactions, modulation of host immune defenses, and regulation of antifungal drug resistance. this study evaluated the in vitro antifungal susceptibility profile to five antifungal agents, the production of seven hydrolytic enzymes related to virulence, and the relationship between these phenotypes in 91 clini ... | 2017 | 28814823 |
| fungicidal activity of copper-sputtered flexible surfaces under dark and actinic light against azole-resistant candida albicans and candida glabrata. | candida spp. are able to survive on hospital surfaces and causes healthcare-associated infections (hcais). since surface cleaning and disinfecting interventions are not totally effective to eliminate candida spp., new approaches should be devised. copper (cu) has widely recognized antifungal activity and the use of cu-sputtered surfaces has recently been proposed to curb the spread of hcais. moreover, the activity of cu under the action of actinic light remains underexplored. we investigated the ... | 2017 | 28802173 |
| monitoring antifungal resistance in a global collection of invasive yeasts and moulds: application of clsi epidemiological cutoff values and whole genome sequencing analysis for detection of azole resistance in candida albicans. | the activity of 7 antifungal agents was evaluated against 3,557 invasive yeasts and moulds collected in 29 countries worldwide during 2014-2015. epidemiological cutoff values (ecvs) published in the clsi m59 document were applied for species with no clinical breakpoints. echinocandin susceptibility rates were 95.9% to 100.0% for the 5 most common candida species except anidulafungin and c. parapsilosis (88.7% susceptible/100.0% wild-type). fluconazole resistance ranged from 8.0% for c. glabrata ... | 2017 | 28784671 |
| the global problem of antifungal resistance: prevalence, mechanisms, and management. | all serious fungal infections need appropriate antifungal therapy for successful patient outcome. only a few classes of antifungal drugs are available, so the emergence of resistance to single drug classes and now multidrug resistance greatly hampers patient management. azole resistance among candida and aspergillus species is one of the greatest challenges to clinical success, followed by echinocandin and multidrug resistance among some candida species, especially candida glabrata. the spread o ... | 2017 | 28774698 |
| prevalence and antifungal susceptibility of candida species in a tertiary care hospital in islamabad, pakistan. | to determine the prevalence and antifungal susceptibility pattern of candida species. | 2017 | 28770873 |
| evaluation of candidemia in epidemiology and risk factors among cancer patients in a cancer center of china: an 8-year case-control study. | candidemia is the worldwide life-threaten disease, especially in cancer patients. this study was aimed to identify and evaluate the risk factors of candidemia in cancer patients, which will prompt the improvement on current therapeutic strategies and prognosis. | 2017 | 28768479 |
| progress in definition, prevention and treatment of fungal infections in cystic fibrosis. | cystic fibrosis (cf) is a chronic lethal multi-system condition; however, most of the morbidity and mortality is dependent on the status of the respiratory system. progressive respiratory decline is mediated by chronic infection and inflammation, punctuated by important acute events known as pulmonary exacerbations which can lead to accelerated decline. the main bacterial species causing infections include pseudomonas aeruginosa, staphylococcus aureus, haemophilus influenzae and achromobacter xy ... | 2017 | 28762125 |
| first reported case of donor related candida endophthalmitis after descemet membrane endothelial keratoplasty. | to report the first case of candida donor to host transmission following descemet membrane endothelial keratoplasty (dmek). | 2017 | 28761565 |
| changes in the distribution of colonising and infecting candida spp. isolates, antifungal drug consumption and susceptibility in a french intensive care unit: a 10-year study. | monitoring fungal ecology and resistance to antifungal agents within intensive care units (icu) is essential for the management of invasive fungal infections. therefore, a retrospective descriptive study was carried in the icu of nimes university hospital, france, from 2007 to 2016. as the majority of invasive fungal infections in icu are caused by candida species, the study objectives were to describe candida species distribution, to assess candidaemia incidence and to monitor the antifungal dr ... | 2017 | 28758337 |
| candida and porphyromonas gingivalis: the effect on wound closure in vitro. | microorganisms play a role in oral mucositis after cancer therapy. the current study explored the hypothesis that candida spp. alone and together with porphyromonas gingivalis cause delayed healing of oral ulcerations due to the inhibition of wound closure. an in vitro scratch assay model was used to study the influence of viable and heat-killed candida glabrata, candida kefyr, and candida albicans on cell migration of oral epithelial cells. separately, the effect of conditioned medium of candid ... | 2017 | 28748033 |
| lumbar spinal candida glabrata treated without surgical intervention: a case report. | candida glabrata is a low virulent commensal fungal organism that, rarely, can cause osteomyelitis. diagnosis of such an infection is often difficult as the case typically presents with an insidious onset of back pain and minimally elevated biomarkers of inflammation. furthermore, it is difficult to eradicate and often resistant to common antifungals. a 61-year-old man presented with an eight-month history of persistent low back pain which had unsuccessfully been managed by his primary care phy ... | 2017 | 28744418 |
| anticandidal activity of extracts and a novel compound, amnomopin, isolated from petriella setifera. | a novel triterpenoidal compound named 'amnomopin' (3β-diglucoside-5,12-28-oic acid), which is named iupac as 3-o-(2' ➔ 1″diglucoside)1,2,3,4,4a,5,6,6a,6b,7,9,10,11,12,12a,12b,13,14b-octadecahydro-10-hydroxy-2,2,6a,6b,9,9,12a-heptamethylpicene-4a-carboxylic acid, was isolated from the extract petriella setifera. the total alcoholic extract of p. setifera showed a great activity against clinically isolated candida species, including candida albicans, candida dubliniensis, candida famata, candida g ... | 2017 | 28730719 |
| emergence of candida glabrata in vulvovaginal candidiasis should be attributed to selective pressure or virulence ability? | vulvovaginal candidiasis (vvc) is one of the most frequent female genital disorders and candida glabrata is the second most common agent. current study was aimed to study the susceptibility to antifungal agents of c. glabrata isolated from vaginal samples and some virulence attributes in order to better understand why this species is emerging as the main vvc agents. | 2017 | 28730269 |
| characterization of culturable yeast species associating with whole crop corn and total mixed ration silage. | this study aims to investigate the association of yeast species with improved aerobic stability of total mixed ration (tmr) silages with prolonged ensiling, and clarify the characteristics of yeast species and their role during aerobic deterioration. | 2017 | 28728388 |
| dectin-1 embraces candida glabrata. | 2017 | 28727942 | |
| mitochondrial cochaperone mge1 is involved in regulating susceptibility to fluconazole in saccharomyces cerevisiae and candida species. | mge1 encodes a yeast chaperone involved in fe-s cluster metabolism and protein import into the mitochondria. in this study, we identified mge1 as a multicopy suppressor of susceptibility to the antifungal fluconazole in the model yeast saccharomyces cerevisiae we demonstrate that this phenomenon is not exclusively dependent on the integrity of the mitochondrial dna or on the presence of the drug efflux pump pdr5. instead, we show that the increased dosage of mge1 plays a protective role by retai ... | 2017 | 28720726 |
| med15b regulates acid stress response and tolerance in candida glabrata. | candida glabrata is a promising producer of organic acids. to elucidate the physiological function of the mediator tail subunit med15b in the response to low ph stress, we constructed a deletion strain, med15bδ, and overexpression strain, htuδ/cgmed15b deletion of med15b caused biomass production, glucose consumption rate, and cell viability to decrease by 28.3%, 31.7%, and 26.5%, respectively, compared with those of the parent strain (htuδ) at ph 2.0. expression of lipid metabolism-related gene ... | 2017 | 28710262 |
| unexpected effects of azole transporter inhibitors on antifungal susceptibility in candida glabrata and other pathogenic candida species. | the pathogenic fungus candida glabrata is often resistant to azole antifungal agents. drug efflux through azole transporters, such as cdr1 and cdr2, is a key mechanism of azole resistance and these genes are under the control of the transcription factor pdr1. recently, the monoamine oxidase a (mao-a) inhibitor clorgyline was shown to inhibit the azole efflux pumps, leading to increased azole susceptibility in c. glabrata. in the present study, we have evaluated the effects of clorgyline on susce ... | 2017 | 28700656 |
| molecular identification and antifungal susceptibility pattern of non-albicans candida species isolated from vulvovaginal candidiasis | vulvovaginal candidiasis (vvc) is an important problem due to candida spp. the aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans candida isolates from vvc. | 2017 | 28688376 |
| evaluation of mir-146a expression level in macrophages exposed to candida glabrata. | micrornas are small non-coding rnas with 19-24 nucleotides in length. up- or down-regulation of many mirnas has been shown by stimulation of toll-like receptors (tlrs) in the innate immune system. up-regulation of mir-146a has been reported by both tlr and heat-killed candida albicans. in this study, we aimed to evaluate the expression of mir-146a in cultured monocyte-derived macrophages (mdms) infected by candida glabrata at 12, 24, and 48 hours. | 2016 | 28681015 |
| evaluation of the antifungal activities of various extracts from pistacia atlantica desf. | despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple treatment-related side-effects. the present study was designed to investigate the antifungal properties of different extracts from pistacia atlantica desf. | 2015 | 28680993 |
| successful treatment of pulmonary candidiasis and aspergillosis in patient with refractory hodgkin lymphoma using micafungin - case study and brief literature review. | the number of patients with hematological malignancies who develop invasive fungal disease (ifd) has increased dramatically in recent decades. this increase is attributed to impairment of the host immune system due to intensive cytotoxic chemotherapies, use of corticosteroids and profound immunosuppression after hematopoietic stem cell transplantation (hsct). additionally, the increasing prevalence of fungal infections caused by emerging and rare pathogens, ifd of mixed etiology or of atypical l ... | 2017 | 28680340 |
| molecular basis of antifungal drug resistance in yeasts. | besides inherent differences in in vitro susceptibilities, clinically relevant yeast species may acquire resistance upon exposure to most antifungal drugs used in the clinic. in recent years, major fundamental research studies have been conducted to improve our understanding of the molecular basis of antifungal resistance. this topic is of major interest as antifungal resistance in yeast is correlated to clinical failure and is clearly evolving. in this minireview, we provide an overview includi ... | 2017 | 28669835 |
| epidemiology of antifungal resistance in human pathogenic yeasts: current viewpoint and practical recommendations for management. | in this review, we describe the epidemiology and clinical significance of resistance in candida spp. and other non-cryptococcus yeasts. the rise in echinocandin resistance, azole resistance and cross-resistance to two or more antifungal classes [multidrug resistance (mdr)] has been a worrisome trend, mainly in us large tertiary and oncology centres, particularly as it relates to candida glabrata. candida kefyr is also a concern as it can be resistant to echinocandins and polyenes, especially in ... | 2017 | 28669831 |
| cgmed3 changes membrane sterol composition to help candida glabrata tolerate low-ph stress. | candida glabrata is a promising microorganism for organic acid production. the present study aimed to investigate the role of c. glabrata mediator complex subunit 3 (cgmed3p) in protecting c. glabrata under low-ph conditions. to this end, genes cgmed3a and cgmed3b were deleted, resulting in the double-deletion cgmed3abδ strain. the final biomass and cell viability levels of cgmed3abδ decreased by 64.5% and 35.8%, respectively, compared to the wild-type strain results at ph 2.0. in addition, lack ... | 2017 | 28667115 |
| functional analysis of selected deletion mutants in candida glabrata under hypoxia. | increased drug resistance in candida glabrata (a model non-albicans candida) calls for the identification of potential molecular targets for the development of effective drugs. hypoxia (a state of low oxygen) is an important host factor, which affects the virulence of the pathogen and efficacy of drugs. in the present study, in vitro characterization of 13 null mutants of c. glabrata were done under hypoxic condition (1% o2). these mutants have a major role to play in cellular pathways, viabilit ... | 2017 | 28664376 |
| comparative genomics of two sequential candida glabrata clinical isolates. | candida glabrata is an important fungal pathogen which develops rapid antifungal resistance in treated patients. it is known that azole treatments lead to antifungal resistance in this fungal species and that multidrug efflux transporters are involved in this process. specific mutations in the transcriptional regulator pdr1 result in upregulation of the transporters. in addition, we showed that the pdr1 mutations can contribute to enhance virulence in animal models. in this study, we were intere ... | 2017 | 28663342 |
| comparative evaluation of six chromogenic media for presumptive yeast identification. | the present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification. | 2017 | 28663328 |
| galleria mellonella as a host model to study candida glabrata virulence and antifungal efficacy. | 2017 | 28658597 | |
| dectin-1 plays an important role in host defense against systemic candida glabrata infection. | candida glabrata is the second most common pathogen of severe candidiasis in immunocompromised hosts, following c. albicans. although c. glabrata and c. albicans belong to the same genus, they are phylogenetically distinct. c-type lectin receptors (clrs), acting as pattern-recognition receptors (prrs), play critical roles in host defense against c. albicans infections. however, our understanding of the specific roles of clrs in host defense against c. glabrata is limited. here, we explored the p ... | 2017 | 28658592 |
| use of rna-protein complexes for genome editing in non-albicans candida species. | clustered regularly interspaced short palindromic repeat (crispr)-cas9 genome modification systems have greatly facilitated the genetic analysis of fungal pathogens. in crispr-cas9 genome editing methods designed for use in candida albicans, dnas that encode the necessary components are expressed in the target cells. unfortunately, expression constructs that work efficiently in c. albicans are not necessarily expressed well in other pathogenic species within the genus candida or the related genu ... | 2017 | 28657070 |
| isolation, speciation and antifungal susceptibility testing of candida isolates from various clinical specimens at a tertiary care hospital, nepal. | candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases along with increased resistance to antifungal drugs has made a serious concern. resistance to antifungal agents has increased during the last decade. thus, identification of candida up to species level and its antifungal susceptibility testing has a paramount significance in the management of candidal infections. the aim of the study was to speciate candida s ... | 2017 | 28646915 |
| genome mining, in silico validation and phase selection of a novel aldo-keto reductase from candida glabrata for biotransformation. | previously, we published cloning, overexpression, characterization and subsequent exploitation of a carbonyl reductase (cr) gene, belonging to general family aldo-keto reductase from candida glabrata cbs138 to convert keto ester (cobe) to a chiral alcohol (ethyl-4-chloro-3-hydroxybutanoate or chbe). exploiting global transcription factor crp, rdna and transporter engineering, we have improved batch production of chbe by trinomial bioengineering. herein, we present the exploration of cr gene in c ... | 2017 | 28644714 |
| the fungal pathogen candida glabrata does not depend on surface ferric reductases for iron acquisition. | iron acquisition is a crucial virulence determinant for many bacteria and fungi, including the opportunistic fungal pathogens candida albicans and c. glabrata. while the diverse strategies used by c. albicans for obtaining iron from the host are well-described, much less is known about the acquisition of this micronutrient from host sources by c. glabrata - a distant relative of c. albicans with closer evolutionary ties to saccharomyces cerevisiae, which nonetheless causes severe clinical sympto ... | 2017 | 28642757 |
| de novo acquisition of resistance to scy-078 in candida glabrata involves fks mutations that both overlap and are distinct from those conferring echinocandin resistance. | scy-078 is an orally active antifungal whose target is the β-(1,3)-d-glucan synthase (gs). we evaluated the spontaneous emergence of scy-078-resistant candida glabrata isolates following drug exposure in vitro resistant isolates were analyzed using broth microdilution methodology and fks sequencing. the kinetic inhibition parameter ic50 (50% inhibitory concentration) was also determined from gs complexes. the spectrum of resistance mutations found suggested a partially overlapping but independen ... | 2017 | 28630180 |
| comparison of the vitek 2 antifungal susceptibility system with etest using clinical isolates of candida species. | candida species are part of the normal human microbiota. however, in recent years, nosocomial bloodstream candida infections have emerged as a significant problem ranking the fourth common cause of fungemia in intensive care units. although microdilution methods are the ones recommended for susceptibility testing, they are difficult to undertake in the clinical practice. thus, an automated commercially available test is ideal. | 2017 | 28622982 |
| antifungal activity of tyrosol and farnesol used in combination against candida species in the planktonic state or forming biofilms. | this study examined the antifungal activity of the combination of tyrosol and farnesol against candida albicans and candida glabrata in the planktonic state or forming biofilms. | 2017 | 28622460 |
| an investigation on non-invasive fungal sinusitis; molecular identification of etiologic agents. | fungal sinusitis is increasing worldwide in the past two decades. it is divided into two types including invasive and noninvasive. noninvasive types contain allergic fungal sinusitis (afs) and fungus ball. afs is a hypersensitivity reaction to fungal allergens in the mucosa of the sinonasal tract in atopic individuals. the fungus ball is a different type of noninvasive fungal rhinosinusitis which is delineated as an accumulation of debris and fungal elements inside a paranasal sinus. fungal sinu ... | 2017 | 28616054 |
| the ccaat-binding complex controls respiratory gene expression and iron homeostasis in candida glabrata. | the ccaat-binding complex (cbc) is a heterotrimeric transcription factor which is widely conserved in eukaryotes. in the model yeast s. cerevisiae, cbc positively controls the expression of respiratory pathway genes. this role involves interactions with the regulatory subunit hap4. in many pathogenic fungi, cbc interacts with the hapx regulatory subunit to control iron homeostasis. hapx is a bzip protein which only shares with hap4 the hap4like domain (hap4l) required for its interaction with cb ... | 2017 | 28615656 |
| invasive candidiasis in brescia, italy: analysis of species distribution and antifungal susceptibilities during seven years. | the aims of this study were to evaluate the epidemiology of nosocomial candidemia in a large teaching hospital in brescia, italy, and the in vitro antifungal susceptibility of isolates. we analyzed 196 isolates causing fungemia in patients admitted in our hospital, between january 2009 and december 2015. strains were identified by vitek 2 and maldi-tof ms. mics were determined by sensititre yeast onetm. the resistance was defined by using the revised clsi breakpoints/epidemiological cutoff value ... | 2017 | 28597394 |
| glabridin triggers over-expression of mca1 and nuc1 genes in candida glabrata: is it an apoptosis inducer? | the growing trends of emergence of antifungal-resistant candida strains has recently been inspired the researchers to design new antifungal agents with novel mechanisms of action. glabridin is an originally natural substrate with multiple biological activities which propose it as a novel anticancer, antimicrobial and antifungal agent. in the present study, the antifungal effect of glabridin against candida glabrata isolates and its possible mechanism of action were investigated. the minimum inhi ... | 2017 | 28595940 |
| clinical validation of a test for the diagnosis of vaginitis. | vaginitis may be diagnosed as bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, or coinfection. a new molecular test assays the vaginal microbiome and organisms that cause three common infections. the objective of the trial was to evaluate the clinical accuracy of the investigational test for vaginal swabs collected by patients (self) or clinicians. the primary and secondary outcomes were to compare the investigational test with reference methods for the three most common causes of ... | 2017 | 28594779 |
| molecular epidemiology and antifungal susceptibility of candida glabrata in china (august 2009 to july 2014): a multi-center study. | candida glabrata is an increasingly important cause of invasive candidiasis. in china, relatively little is known of the molecular epidemiology of c. glabrata and of its antifungal susceptibility patterns. here we studied 411 non-duplicate c. glabrata isolates from 411 patients at 11 hospitals participating in the national china hospital invasive fungal surveillance net program (chif-net; 2010-2014). genotyping was performed using multilocus sequence typing (mlst) employing six genetic loci and ... | 2017 | 28588560 |
| evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms. | scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus). | 2017 | 28587870 |
| initial treatment of cancer patients with fluconazole-susceptible dose-dependent candida glabrata fungemia: better outcome with an echinocandin or polyene compared to an azole? | the 28-day crude mortality rate in 68 cancer patients with fluconazole-susceptible dose-dependent candida glabrata fungemia started on treatment (within 48 h after blood culture collection) with an echinocandin or liposomal amphotericin-b was better (30%) than those treated with azole monotherapy (52%) (p = 0.07). after adjusting for confounders, azole monotherapy also was associated with worse 28-day survival (hazard ratio, 3.8; p = 0.003). | 2017 | 28584138 |
| vaginal candida spp. genomes from women with vulvovaginal candidiasis. | candida albicans is the predominant cause of vulvovaginal candidiasis (vvc). little is known regarding the genetic diversity of candida spp. in the vagina or the microvariations in strains over time that may contribute to the development of vvc. this study reports the draft genome sequences of four c. albicans and one c. glabrata strains isolated from women with vvc. an snp-based whole-genome phylogeny indicates that these isolates are closely related; however, phylogenetic distances between the ... | 2017 | 28582496 |
| [candida glabrata vaginitis: the great ignored?] | 2017 | 28579083 | |
| the essential oil of allium sativum as an alternative agent against candida isolated from dental prostheses. | the colonization of the surfaces of dental prostheses by candida albicans is associated with the development of denture stomatitis. in this context, the use of fluconazole has been proposed, but its disadvantage is microbial resistance. meanwhile, the oil of allium sativum has shown an effect in controlling biofilm formation by c. albicans. | 2017 | 28578870 |
| synthesis, characterization, and in vitro activity against candida spp. of fluconazole encapsulated on cationic and conventional nanoparticles of poly(lactic-co-glycolic acid). | in this study, nanoparticles (nps) of poly(lactic-co-glycolic acid) (plga) loaded with fluconazole (flz) and flz-nps coated with the cationic polymer polyethylenimine (pei) (flz-np-pei) were synthetized in order to improve antimycotic activity against four strains of candida spp. of clinical relevance. flz-nps and flz-np-pei were synthesized by double emulsion solvent-diffusion (des-d) and characterized. minimum inhibitory concentration (mic50) and minimum fungicide concentration (mfc) were dete ... | 2017 | 28572725 |
| auxanographic carbohydrate assimilation method for large scale yeast identification. | the auxanographic carbohydrate assimilation had been an important method for differentiation of yeasts. prevailing methods described in the literature for carbohydrate assimilation has limited scope for use in large scale yeast identification. | 2017 | 28571137 |
| promising therapies for fungal infection based on the study to elucidate mechanisms to cope with stress in candida species [translated article]. | in recent years, the incidence of fungal infections has been increasing, particularly among patients with immune systems compromised by human immunodeficiency virus infection, organ transplantation, and/or chemotherapy for cancer. current therapies for treating systemic fungal infection have limited effectiveness and have created problems of adverse reactions and drug resistance. these issues therefore motivate us to develop novel antifungals. elucidation of stress response mechanisms and virule ... | 2017 | 28566663 |
| mismatch repair of dna replication errors contributes to microevolution in the pathogenic fungus cryptococcus neoformans. | the ability to adapt to a changing environment provides a selective advantage to microorganisms. in the case of many pathogens, a large change in their environment occurs when they move from a natural setting to a setting within a human host and then during the course of disease development to various locations within that host. two clinical isolates of the human fungal pathogen cryptococcus neoformans were identified from a collection of environmental and clinical strains that exhibited a mutat ... | 2017 | 28559486 |
| cellular conditions that modulate the fungicidal activity of occidiofungin. | to identify cellular conditions that significantly alter susceptibility of saccharomyces cerevisiae, candida albicans and candida glabrata to the antimicrobial peptide, occidiofungin. | 2017 | 28556561 |
| invasive fungal infections following liver transplantation. | the review outlines the microbiology, presentation, prophylactic strategies, resistance patterns, and consequences of invasive fungal infections (ifis) in orthotopic liver transplantation (olt) recipients. | 2017 | 28548995 |
| heteroleptic metal(ii) complexes of hydrotris(methimazolyl)borate and diimines: synthesis, theoretical calculations, antimicrobial, antioxidant, in vitro cytotoxicity and molecular docking studies. | a series of heteroleptic metal(ii) complexes of formulation [m(tm)(diimine)](clo4) (1-8), [tm = hydrotris(methimazolyl)borate, diimine = 2,2'-bipyridyl or 1,10-phenanthroline and m = mn(ii), ni(ii), cu(ii) or zn(ii)] have been synthesized and characterized by spectroscopic methods. the geometric parameters of the complexes were determined using uv-vis spectroscopy and dft calculations. the analyses of homo and lumo have been used to explain the charge transfer within the molecule. antimicrobial ... | 2017 | 28546116 |
| [common genital infections]. | vulvo-vaginal infections are frequently encountered by gynecologists and general practitioners. the diagnosis and the recurrence of these affections must lead to a discussion about sexually transmitted disease, notably when trichomonas vaginalis and herpetic infections are found. even if they do not correspond to classical sexually transmitted diseases, their prevalence is increased by sexual intercourses reports and the number of partners which should also be treated. it is necessary to documen ... | 2016 | 28525226 |
| usefulness of the non-conventional caenorhabditis elegans model to assess candida virulence. | invasive candidiasis is caused mainly by candida albicans, but other candida species have increasing etiologies. these species show different virulence and susceptibility levels to antifungal drugs. the aims of this study were to evaluate the usefulness of the non-conventional model caenorhabditis elegans to assess the in vivo virulence of seven different candida species and to compare the virulence in vivo with the in vitro production of proteinases and phospholipases, hemolytic activity and bi ... | 2017 | 28523422 |
| fungal profile of vulvovaginal candidiasis in a tertiary care hospital. | vulvovaginal candidiasis (vvc) is a common medical health problem of adult women. it is most commonly caused by candida albicans. but there is a change in fungal profile. sabouraud's dextrose agar (sda) is the most common culture medium used where mixed fungal infection may be missed. it can be detected easily by using chromogenic culture medium. | 2017 | 28511380 |
| candidiasis: prevalence and resistance profiling in a tertiary care hospital of pakistan. | to determine candida colonisation/infection in renal transplant patients and to determine the resistance pattern against antifungal drugs. | 2017 | 28507352 |
| co2 sensing in fungi: at the heart of metabolic signaling. | adaptation to the changing environmental co2 levels is essential for all living cells. in particular, microorganisms colonizing and infecting the human body are exposed to highly variable concentrations, ranging from atmospheric 0.04 to 5% and more in blood and specific host niches. carbonic anhydrases are highly conserved metalloenzymes that enable fixation of co2 by its conversion into bicarbonate. this process is not only crucial to ensure the supply of adequate carbon amounts for cellular me ... | 2017 | 28493119 |
| activity of combined antifungal agents against multidrug-resistant candida glabrata strains. | in this study, we evaluated the in vitro activity of echinocandins, azoles, and amphotericin b alone and in combination against echinocandin/azole-sensitive and echinocandin/azole-resistant candida glabrata isolates. susceptibility tests were performed using the broth microdilution method in accordance with the clinical and laboratory standards institute document m27-a3. the checkerboard method was used to evaluate the fractional inhibitory concentration index of the interactions. cross-resistan ... | 2017 | 28493006 |
| rapid emergence of fks mutations in candida glabrata isolates in a peritoneal candidiasis. | we report a rapid acquisition of echinocandin resistance after 12 days of micafungin treatment, without prior exposure, in a patient with peritoneal candidiasis due to c. glabrata. isolates recovered before and after treatment were compared by multilocus sequence typing. results of antifungal susceptibility testing and fks mutations were reported. the interest of repeating antifungal susceptibility testing for echinocandin molecules during the treatment is discussed and a strategy to research fk ... | 2017 | 28491490 |
| generational distribution of a candida glabrata population: resilient old cells prevail, while younger cells dominate in the vulnerable host. | similar to other yeasts, the human pathogen candida glabrata ages when it undergoes asymmetric, finite cell divisions, which determines its replicative lifespan. we sought to investigate if and how aging changes resilience of c. glabrata populations in the host environment. our data demonstrate that old c. glabrata are more resistant to hydrogen peroxide and neutrophil killing, whereas young cells adhere better to epithelial cell layers. consequently, virulence of old compared to younger c. glab ... | 2017 | 28489916 |
| monitoring of iron depletion-induced mitophagy in pathogenic yeast. | mitophagy, which is the degradation of mitochondria via selective autophagic machinery, is thought to be involved in regulating the mass and function of mitochondria. methods for detection of mitophagy have been reported for several fungal cells including some budding yeast, methylotrophic yeast, and filamentous fungi. mitophagy in saccharomyces cerevisiae is activated under nitrogen-poor conditions; however, the regulatory mechanism of mitophagy in most fungi has not been elucidated. here we de ... | 2017 | 28488243 |
| genotyping of clinical isolates of candida glabrata from iran by multilocus sequence typing and determination of population structure and drug resistance profile. | candida glabrata is often the second most common causative agent for candidiasis following candida albicans. despite the importance of c. glabrata infections, few epidemiological studies have been conducted on this issue. the goal of this study was genotyping of clinical isolates of c. glabrata by multilocus sequence typing (mlst) technique for determination of the endemic prevalent genotypes and any association between isolation source and drug resistance. a total of 50 c. glabrata clinical iso ... | 2017 | 28482076 |
| an in vitro study of isolation of candidal strains in oral squamous cell carcinoma patients undergoing radiation therapy and quantitative analysis of the various strains using chromagar. | patients undergoing radiation therapy for oral squamous cell carcinoma (oscc) have an increased risk of developing oral candidiasis. radiation-induced hyposalivation is thought to be a major predisposing factor for it. radiation therapy for 2-3 weeks leads to increase in the number of candidal species in the oral cavity, eventually leading to oral candidiasis. | 2017 | 28479680 |
| candida bezoars in adults: determining optimal management. | fungal bezoars, or fungal balls, are rare pathologic consequences of funguria in immunocompromised patients. current treatment recommendations are based on expert opinion and low level evidence. we present a case of a candida glabrata bezoar that was effectively treated with percutaneous amphotericin b instillations. a subsequent literature review is presented to assess the available case reports and treatment outcomes of candida spp. bezoars in adults. | 2017 | 28466076 |
| the effect of fermented lingonberry juice on candida glabrata intracellular protein expression. | lingonberries have a long traditional use in treating fungal infections on mucosal membranes, but very little is known about the exact antifungal mechanisms. we tested the effects of fermented lingonberry juice on candida glabrata intracellular protein expression. a candida glabrata clinical strain was grown in the presence of fermented lingonberry juice (flj). also the effect of lowered ph was tested. intracellular protein expression levels were analyzed by the 2d-dige method. six proteins dete ... | 2017 | 28465686 |
| phenotypic and molecular evaluation of echinocandin susceptibility of candida glabrata, candida bracarensis, and candida nivariensis strains isolated during 30 years in argentina. | the echinocandin susceptibilities of 122 candida glabrata complex strains (including 5 candida nivariensis and 3 candida bracarensis strains) were evaluated by microdilution and compared with the results from a molecular tool able to detect fks mutations. no echinocandin resistance was detected. the pcr results coincide with the mic data in 99.25% of the cases (1 c. glabrata strain was misidentified as resistant) but were 20 h faster. c. nivariensis fks genes were sequenced and showed difference ... | 2017 | 28461313 |
| efficacy of anidulafungin in 539 patients with invasive candidiasis: a patient-level pooled analysis of six clinical trials. | to evaluate the efficacy of anidulafungin for the treatment of candidaemia and invasive candidiasis in a large dataset, including patients with deep-seated tissue candidiasis, neutropenia and infection due to non- albicans candida species. | 2017 | 28459966 |
| dna interaction, antimicrobial, anticancer activities and molecular docking study of some new vo(ii), cr(iii), mn(ii) and ni(ii) mononuclear chelates encompassing quaridentate imine ligand. | the present study was conducted to synthesis of some new imine cr(iii), vo(ii), mn(ii) and ni(ii) complexes derived from the condensation of 2-amino phenol with 2-hydroxynapthaldehyde were synthesized. the prepared hnpn imine ligand was analyzed by its melting point, ir, (1)h nmr and (13)c nmr spectroscopies. the investigated hnpn imine complexes were characterized by elemental analysis, ft ir, uv-vis and thermal analysis (tga) under nitrogen atmosphere from ambient temperature to 750°c. the exp ... | 2017 | 28456118 |
| the effectiveness of voriconazole in therapy of candida glabrata's biofilms oral infections and its influence on the matrix composition and gene expression. | candida glabrata is one of most prevalent yeast in fungal infections, especially in immunocompromised patients. its azole resistance results in a low therapeutic response, particularly when associated with biofilms. the main goal of this work was to study the effectiveness of voriconazole (vcz) against c. glabrata biofilms oral pathologies, as esophageal or oropharyngeal candidiasis. antifungal susceptibilities were determined in pre-formed 24-h-biofilms and erg genes expression was determined b ... | 2017 | 28439794 |
| the antibiotic polymyxin b exhibits novel antifungal activity against fusarium species. | the genus fusarium comprises many species, including fusarium oxysporum, fusarium solani, fusarium graminearum and fusarium verticillioides, and causes severe infections in plants and humans. in clinical settings, fusarium is the third most frequent mould to cause invasive fungal infections after aspergillus and the mucorales. f. solani and f. oxysporum are the most prevalent fusarium spp. causing clinical disease. however, few effective antifungal drugs are available to treat human and plant fu ... | 2017 | 28433743 |
| synergistic anticandidal activity of menthol in combination with itraconazole and nystatin against clinical candida glabrata and candida krusei isolates. | candida glabrata (c. glabrata) and c. krusei are now emerging as serious hospital acquired infections in immunocompromised patients. menthol, a terpenic compound, has been reported to have antifungal activity. | 2017 | 28431915 |
| oral mycobiome in community-dwelling elderly and its relation to oral and general health conditions. | oral fungal infection is generally associated with dysbiosis related to antibiotic use, immunodeficiency, or frailty. however, fungal colonization in a typical population without apparent symptoms and its associated conditions are poorly understood. in this study, oral fungal colonization in community-dwelling and independently living elderly populations was evaluated and factors affecting fungal colonization were analyzed. | 2017 | 28419681 |
| absolute eosinophil count was significantly associated with serum (1,3)-β-d-glucan in patients with invasive fungal infections. | measurement of serum (1,3)-β-d-glucan has emerged as an important diagnostic strategy for invasive fungal infections (ifi). previous studies suggested that the eosinophil count would be related with the value of serum (1,3)-β-d-glucan. | 2017 | 28397466 |
| spontaneous fungal peritonitis in ascites of cardiac origin. | spontaneous fungal peritonitis (sfp) is an infrequent but severe complication most commonly described in patients with liver cirrhosis. we present the first case of culture-proven sfp occurring in cardiogenic ascites. the diagnosis of sfp was clinically challenging as the initial ascites was consistent with the more common diagnosis of spontaneous bacterial peritonitis (sbp). the patient did not respond to antibacterial therapy, however, and the final diagnosis was only made with positive asciti ... | 2017 | 28386572 |
| fungal opportunistic pneumonias in hiv/aids patients: an indian tertiary care experience. | opportunistic pneumonias are a major cause of mortality and morbidity in human immunodeficiency virus (hiv) reactive patients. despite the significant role that fungi play in causation of this opportunistic mycoses, very few indian studies have attempted to investigate the burden and aetiological spectrum of hiv/aids-associated fungal pneumonias. | 2017 | 28384860 |
| a case of breakthrough candida parapsilosis fungemia during micafungin therapy for a candida glabrata bloodstream infection. | we describe a case of breakthrough candida parapsilosis fungemia in an 80-year-old woman with pyoderma gangrenosum and rheumatoid arthritis. c. parapsilosis was detected in blood culture while the patient was treated with micafungin for a candida glabrata bloodstream infection. the breakthrough infection was successfully treated with liposomal amphotericin b. | 2017 | 28377868 |