Publications

pea (pisum sativum l.) seed isolectins 1 and 2 and pea root lectin result from carboxypeptidase-like processing of a single gene product.	the complete amino acid sequences of the alpha-subunits of pea (pisum sativum l.) seed and root lectin, the c-terminal amino acids of the beta-subunits of pea seed lectin, and most of the sequence of the beta-subunit of pea root lectin were determined. in contrast to earlier reports it was shown that the beta-subunits of both seed isolectins end at asn-181. the alpha 1 subunits end at gln-241 (major fraction) or lys-240 (minor fraction), whereas the alpha 2 subunits end at ser-239, ser-238, ser- ...	1994	8111028
production of extracellular xylanases by penicillium janthinellum. effect of selected growth conditions.	xylanase production by penicillium janthinellum using 10-100 mm of 2,2-dimethylsuccinate (dms) buffer, in a range of ph 4.5-6.0 was studied. the enzyme activity was enhanced using oat xylan as the carbon source. under these conditions a culture produced 1.14 mumol/min (11.4 u/ml or 84.4 u/mg) of beta-xylanase after 5 d of growth in a 10-mm buffer solution at ph 4.5. protease was absent in the dms buffer except when 100 mm phosphate buffer at ph 6.0 was used (4 u/ml). beta-xylosidase was only fou ...	1994	7944349
novel serine penicillocarboxypeptidase cpd-s3 from penicillium janthinellum ibt 3991: purification, characterization, and uses in peptide synthesis and modification.	a novel carboxypeptidase (cpd-s3) from penicillium janthinellum ibt 3991 has been isolated in a two-step purification procedure by cation exchange and affinity chromatography. the enzyme is a serine carboxypeptidase with a denatured molecular mass determined by sds of 62 kda of which 32% is carbohydrate. the isoelectric point is 5.1. cpd-s3 exhibits a high stability towards organic solvents and elevated temperatures. besides the carboxypeptidase activity, cpd-s3 exhibits esterase, amidase, and c ...	1993	7764295
the primary structure of carboxypeptidase s3 from penicillium janthinellum ibt 3991.	the complete amino acid sequence of penicillopeptidase s3, a serine carboxypeptidase isolated from penicillium janthinellum ibt 3991, has been determined. the enzyme consists of 481 amino acids arranged in a single polypeptide chain. six glycosylation sites were established in positions 41, 218, 256, 326, 384 and 392. the molecule contains six cysteinyl residues among which disulfide bridges was established between cys-71-cys-333 and cys-233-cys-289. carboxypeptidase s3 is homologous to carboxyp ...	1995	7664873
crystal structure of catalase hpii from escherichia coli.	catalase is a ubiquitous enzyme present in both the prokaryotic and eukaryotic cells of aerobic organisms. it serves, in part, to protect the cell from the toxic effects of small peroxides. escherichia coli produces two catalases, hpi and hpii, that are quite distinct from other catalases in physical structure and catalytic properties. hpii, studied in this work, is encoded by the kate gene, and has been characterized as an oligomeric, monofunctional catalase containing one cis-heme d prosthetic ...	1995	7663946
the oxidation of pyrene and benzo[a]pyrene by nonbasidiomycete soil fungi.	the purpose of this study was to determine the ability of nonbasidiomycete soil fungi to oxidize pyrene (four rings) and benzo[a]pyrene (bap) (five rings). fungi were isolated from five different soils in which the polycyclic aromatic hydrocarbon content ranged from 0.8 to 80 micrograms/g dry soil. approximately 50% of the isolates in all sites were able to oxidize pyrene. the pyrene-oxidizing species belonged to all fungal divisions except basidiomycetes. the most common were penicillium spp. o ...	1995	7627908
[formation of coenzyme vitamins and flavin-adenine dinucleotide during the growth of penicillium vitale pidopl. et bilai].		2006	7432204
[effect of microelements on growth of penicillium vitale pidopl. et bilai and synthesis of a number of extracellular enzymes].		2006	7402104
the janthitrems: fluorescent tremorgenic toxins produced by penicillium janthinellum isolates from ryegrass pastures.	new tremorgenic mycotoxins named janthitrem a, b, and c (molecular weights 601, 585, and 569, respectively) were produced by more than half of 21 penicillium janthinellum isolates obtained from ryegrass pastures involved in ryegrass staggers outbreaks in sheep.	1980	7356319
[comparative kinetics of reactions catalyzed by glucose oxidase in the presence of different electron acceptors].	it was shown that six redox indicators, beside oxygen, can be used as substrates for glucose oxidase from penicillium vitale. the ph dependence of the rate of glucose oxidase-catalyzed reactions in the presence of oxygen and artificial electron acceptors was studied. in contrast to the reaction involving oxygen, in which the maxima of the ph activity profile were observed at ph 5.6, glucose oxidase reveals maximal ph activity profile at ph 7.5 -- 7.6 in the presence of phenasine methosulfate, ba ...	1981	7284485
[stability of penicillium vitale immobilized catalase in continuous decomposition of hydrogen peroxide].	the process of hydrogen peroxide continuous decomposition by the preparation of the fungus penicillium vitale catalase immobilized by aminoorganosilica which were activated by glutaric aldehyde, cyanuric chloride or 2,4-toluylene diisocyanate. catalase with an oxidized carbohydrate component was used as well. such a modified enzyme was directly bound with the surface of aminocontaining silica and alumina. it is shown that in the process of h2o2 decomposition the preparations of immobilized catal ...	2015	7281255
[immobilization of penicillium vitale catalase on aminoethyl cellulose and properties of the obtained preparations].	preparations of penicillium vitale catalase immobilized by aminoethyl cellulose (ae-cellulose) are obtained using two methods: by the enzyme covalent cross-linking with the carrier by glutaric aldehyde and by the covalent binding of catalase to the carrier aminogroups through the carbohydrate enzyme component. a dependence is established for the degree of catalase binding and catalase activity of the immobilized enzyme on the enzyme carrier in immobilization ratio. the optimal enzyme-carrier rat ...	2015	7281254
[immobilization of penicillium vitale glucose oxidase by aminoethyl cellulose and properties of immobilized enzyme].	penicillium vitale glucose oxidase modified by oxidation of the carbohydrate component is in a covalent combination with aminoethyl cellulose (ae-cellulose). with the optimal enzyme: carrier ratio the manifested activity of glucose oxidase in the preparation is 4.3 +/- 0.8% of the attached enzyme activity. it is established that thermostability, stability to an inactivating effect of a labilizing fraction and ph-stability with ph alkaline values in the immobilized glucose oxidase are higher than ...	2015	7281253
repression of endo-1,4-beta-glucanase formation in penicillium janthinellum and product inhibition of its 1,4-beta-glucanases and cellobiases.	endo-1,4-beta-glucanase formation of penicillium janthinellum was repressed by glucose, sophorose, and glycerol. chromatography on deae-sephadex a-50 was employed to separate the 1,4-beta-glucanases from two cellobiases. the 1,4-beta-glucanases were inhibited competitively by cellobiose and glucose, and the two cellobiases were inhibited by glucose and glucono-delta-lactone.	1982	6799497
[application of ultrafiltration for concentration and purification of penicillium vitale catalase].	various factors are studied for their effect on the ultrafiltration of penicillium vitale catalase. it is established that acetate cellulose and polyamide membranes may be used for additional purification and deep concentration of native preliminarily purified enzyme solutions. membranes yam-300 and yam-450 are most preferable. at the temperature of 10 degrees c, pressure 0.2 mpa and 100-fold concentration of the catalase solution the enzyme yield at the ultrafiltration stage is 96-100%.	2015	6636309
[immobilization of penicillium vitale pidopl. et bilai catalase by inorganic carriers].	an efficient method is developed for p. vitale catalase immobilization through the oxidized carbohydrate enzyme component, using silochrome. the method provides the enzyme binding without losing its catalytic capacity in the immobilized preparation. when the enzyme is immobilized by high-dispersed silica containing isocyanate, aldehyde groups or active atoms of chlorine, 8, 15, and 20 mg of the enzyme is bounded per 1 g of the carrier, respectively, its catalytic capacity being completely retain ...	2015	6266097
growth of penicillium janthinellum on glycine as sole carbon and nitrogen source.	penicillium janthinellum is able to grow on glycine as the sole carbon and nitrogen source. the amino acid is transaminated to glyoxylate which is further metabolised to pyruvate by the glycerate pathway. the reaction product of partially purified glycerate kinase from this fungus is 2-phosphoglycerate. phosphoglycerate mutase initiates gluconeogenesis from glycine. partially purified phosphoglycerate mutase is inhibited by fructose 6-phosphate. the possible significance of this regulation is di ...	1980	6251917
the role of a protease in sporulation of penicillium janthinellum.		1966	5961655
[on the mechanism of the stimulatory effect of calcium carbonate on the glucose oxidase and catalase activities of penicillium vitale].		2013	5871568
[on some properties of crystalline and purified non-crystalline glucose-oxidase preparations from penicillium vitale pidopl. et bilai].		1965	5869922
[glucosooxidase from penicillium vitale pidopl. and bilai].		2000	5733674
[natural variability of the strain penicillium janthinellum biourge--producer of janthinellin].		1968	5707360
[composition and structure of glucose oxidase from penicillium vitale].		2013	5556037
[role of inoculum in formation of glucose oxidase and catalase of the penicillium vitale fungus].		2000	5519073
large-scale preparation and some properties of penicillopepsin, the acid proteinase of penicillium janthinellum.		1970	5418964
a crystalline proteinase (peptidase a) from penicillium janthinellum: preliminary x-ray data.		1969	5346053
[the effect of the quality of the sowing material on the enzyme activity and flavinogenesis of penicillium vitale pidopl. et bilai under industrial conditions].		2015	5153983
[conditions of direct biosynthesis of catalase by penicillium vitale pidopl. et bilai].		2001	5153535
a pepsin-like enzyme from penicillium janthinellum.		1968	4866867
[study of the amino acid makeup of the proteins in the mycelial pellicle of penicillium vitale pid. et bil].		2006	4840331
[hydrodynamic properties and molecular weight of penicillium vitale catalase].		2016	4823742
[composition and properties of the catalase from penicillium vitale pidopl. et bilai].		2000	4790749
influence of inorganic nitrate on the formation of extracellular protease and ribonuclease by penicillium janthinellum.		1973	4762798
[study of tubular crystals of penicillium vitale glucose oxidase and its quaternary structure].		1973	4754779
[formation of glucose oxidase and catalase during the growth of penicillium vitale pidopl. et bilai on media with different nitrogen and carbon ratios].		1972	4667024
[obtaining purified preparations of catalase from the fungus penicillium vitale pidopl. et bilai].		1972	4661073
submerged production, purification, and crystallization of acid carboxypeptidase from penicillium janthinellum ifo-8070.	penicillium janthinellum ifo-8070 produced an acid carboxypeptidase of molecular weight 51,000 in a liquid medium at 25 c. maximum enzyme concentration was obtained within 3 to 6 days in a medium containing 2% wheat bran, 1% defatted soybean, and 1% kh(2)po(4); the initial ph was 2 to 4. when submerged aerobic conditions were used, a 51,000-molecular-weight acid carboxypeptidase was produced and no detectable amounts of 160,000-molecular-weight acid carboxypeptidase were produced. acid carboxype ...	1974	4474829
[variability of penicillium janthinellum biourge--producer of the antibiotic janthinellin and study of the nuclei during ontogenesis of the fungus].		2008	4453214
[carbohydrate components of the glucose oxidase from penicillium vitale].		2013	4441570
purification and properties of an extracellular acid ribonuclease from penicillium janthinellum.		1974	4371002
the active center of catalase.	the refined structure of beef liver catalase (i. fita, a. m. silva, m. r. n. murthy & m. g. rossmann, unpublished results) is here examined with regard to possible catalytic mechanisms. the distal side of the deeply buried heme pocket is connected with the surface of the molecule by one (or possibly two) channel. the electron density representing the heme group, in each of the two crystallographically independent subunits, is consistent with degradation of the porphyrin rings. the heme group app ...	1985	4046038
[study of the subunit structure of catalase from penicillium vitale].	a molecule of penicillium vitale catalase is shown to dissociate into subunits with the molecular weight 75-80 kdalton. when hemin is splitted off the molecule also disintegrates into subunits equalling 1/4 of the enzyme molecule. the amino acid composition and fingerprints of the catalase subunits were studied. it is supposed that n-terminal residue of the subunit is blocked.	2015	4035792
penicillopepsin, the aspartic proteinase from penicillium janthinellum: substrate-binding effects and intermediates in transpeptidation reactions.		1985	3912235
action of serine carboxypeptidases on endopeptidase substrates, peptide-4-methyl-coumaryl-7-amides.	carboxypeptidase y hydrolyzed n-substituted peptide-4-methylcoumarin-7-amides (peptide-nh-mec) at ph 7 by releasing 7-amino-4-methylcoumarin (nh2-mec) which was then followed by carboxypeptidase action. in particular, a chymotrypsin-directed substrate, suc-leu-leu-val-tyr-nh-mec, was hydrolyzed by the enzyme with a second-order rate constant of 7200 m-1 s-1, which is compatible with the rate for an anilide substrate and some n-substituted dipeptides. the activity was completely inhibited by phen ...	1985	3905405
autoradiographic method to screen for soil microorganisms which accumulate zinc.	an autoradiographic method was developed to screen for and isolate soil microorganisms which accumulate zinc (zn). diluted soil samples (rubicon fine sand, entic haplorthods [ph 5.9]) were plated on soil extract-glucose agar containing radioactive 65zn. after 7 days of incubation, individual colonies which accumulated sufficient 65zn could be detected by autoradiography. these colonies were isolated and confirmed as zn accumulators in pure culture by using the autoradiographic plate technique. m ...	1985	3883897
the nadph binding site on beef liver catalase.	beef liver and human erythrocyte catalases (ec 1.11.1.6) bind nadp tenaciously [kirkman, h. n. & gaetani, g. f. (1984) proc. natl. acad. sci. usa 81, 4343-4348]. the position of nadp on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. in contrast to nearly all other known structures of protein-bound nadp, nad, and fad, the nadp molecule of beef l ...	1985	3856839
comparison of beef liver and penicillium vitale catalases.	the structures of penicillium vitale and beef liver catalase have been determined to atomic resolution. both catalases are tetrameric proteins with deeply buried heme groups. the amino acid sequence of beef liver catalase is known and contains (at least) 506 amino acid residues. although the sequence of p. vitale catalase has not yet been determined chemically, 670 residues have been built into the 2 a resolution electron density map and have been given tentative assignments. a large portion of ...	1986	3712444
three-dimensional structure of catalase from penicillium vitale at 2.0 a resolution.	the three-dimensional structure analysis of crystalline fungal catalase from penicillium vitale has been extended to 2.0 a resolution. the crystals belong to space group p3(1)21, with the unit cell parameters of a = b = 144.4 a and c = 133.8 a. the asymmetric unit contains half a tetrameric molecule of 222 symmetry. each subunit is a single polypeptide chain of approximately 670 amino acid residues and binds one heme group. the amino acid sequence has been tentatively determined by computer grap ...	1986	3712443
nucleotide sequence of the saccharomyces cerevisiae ctt1 gene and deduced amino-acid sequence of yeast catalase t.	a 2642-base-pair dna fragment containing the catalase t (ctt1) structural gene of the yeast saccharomyces cerevisiae and its flanking regions has been sequenced. the gene codes for a protein of 562 amino acids (relative molecular mass 64,449) and appears to contain no intron. the amino acid sequence of catalase t derived from the dna sequence shows 40.7% homology (52.2% including conservative replacements) to that of bovine liver catalase. all amino acids previously postulated to participate dir ...	1986	3536508
amino acid sequence of endothiapepsin. complete primary structure of the aspartic protease from endothia parasitica.	the amino acid sequence of endothiapepsin, the aspartic protease from endothia parasitica has been determined. the enzyme consists of 330 residues. the sequence determination was performed exclusively at the protein level. the homology of this fungal milk-clotting enzyme with aspartic proteases is demonstrated by alignment with pepsin, chymosin, gastricsin, renin, and cathepsin d from various vertebrates and proteinase a from saccharomyces cerevisiae showing 25-30% identity. the identity with mu ...	1987	3305016
carboxypeptidase s-1 from penicillium janthinellum: enzymatic properties in hydrolysis and aminolysis reactions.	carboxypeptidase s-1 from penicillium janthinellum has been isolated by affinity chromatography and characterized. the enzyme activity is unusually stable in organic solvents, e.g. 80% methanol. the hydrolysis of peptide substrates is apparently dependent on three ionizable groups. one group, with pka of 4.0-4.5, is a catalytically essential residue in its deprotonated form, and another group with a pka of 6.5-7.0 functions in its protonated form, apparently as the binding site for the c-termina ...	1988	3256309
comparative spectral analysis of mammalian, fungal, and bacterial catalases. resonance raman evidence for iron-tyrosinate coordination.	resonance raman spectra are reported for catalases from bovine liver, the ascomycete fungus aspergillus niger, and the bacterium micrococcus luteus. the vibrational frequencies of the oxidation-, spin-, and coordination number-sensitive spectral bands are indicative of high spin pentacoordinate hemes in the resting ferric enzymes of each of these organisms. this result is in accord with the crystal structure of bovine catalase (fita, i., and rossmann, m.g. (1985) j. mol. biol. 185, 21-37). in co ...	1989	2753885
electrophoretic karyotype of cellulolytic penicillium janthinellum strains.	the genome of the cellulolytic fungus penicillium janthinellum biourge was resolved completely by rotating field electrophoresis. the gel pattern revealed 8-10 different chromosomes. on the basis of data for yeast chromosome size standards from schizosaccharomyces pombe and saccharomyces cerevisiae, chromosome sizes in the range from 2.0 to 8 mb were estimated. by southern hybridization with heterologous probes, the chromosomal locations of rdna, the elongation factor ef1, actin and ubiquitin ha ...	1991	1934133
differentiation of species and strains among filamentous fungi by dna fingerprinting.	we have analyzed 11 strains and clones, representing five species (penicillium janthinellum, p. citrioviridae, p. chrysogenum, aspergillus niger, trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the dna of phage m13. the oligonucleotide probes (ct)8, (gtg)5 and (gaca)4, as well as m13 dna, are informative probes for fingerprinting in all genera and species tested. the pro ...	1991	1907892
laminarinase from penicillium funiculosum and its role in release of beta-glucosidase.	an extracellular laminarinase (1----3)-beta-glucan glucohydrolase (ec 3.2.1.6) was purified from culture filtrates of penicillium funiculosum. it was homogeneous on polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. it had a mr of 14,000 and isoelectric point of ph 4.2. the apparent km value for lamimarinase was 8.3 mg/ml and vmax was 8 mumol/min/mg. the distribution of beta-glucosidase activity in two different species of penicillium showed that p. funicul ...	1991	1904246
[identification of toxigenic mould in soft drink causing food poisoning].	in a soft drink caused food poisoning, white floccus was found and mould count was 6.0 x 10(2) cfu/ml. the mycoflora was made of only one kind of mould which was identified as penicillium janthinellum biourge. this isolate can grow under anaerobic condition. the culture liquid of the isolate was fed to mice orally for toxicity test, which made the mice lose weight. an extract of the culture liquid was tested in weaned mice inaberitoneally for toxicity and all mice died in 24h. the toxic symptoms ...	1992	1606874
[dermatomycoses in workers in enterprises of the microbiological industry].	mycologic examination of 54 patients with clinical manifestations of dermatomycosis, engaged in glucose oxidase and catalase production has found a producer fungus, penicillium vitale in 31 (38%); 3 out of 37 people contacting with producers of glucamylase and pectofetidine appeared to be infected by aspergillus awamori (1.1%) and aspergillus foetidus (1.1%), respectively. the producers were isolated from the skin of 40 (16.44%) out of 243 workers having no clinical signs of dermatomycosis, main ...	1992	1427292
microbial degradation of the thiolcarbamate herbicide, diallate, in soils and by pure cultures of soil microorganisms.	the disappearance of the herbicide, avadex (40% diallate), from five agricultural soils (differing in either ph, carbon content, or nitrogen content), incubated under sterile and non-sterile conditions, was followed for a period of 20 weeks. avadex was rapidly lost from microbiologically active soils, with over 50% of the applied (2.5 ppm) dosage disappearing within four weeks; losses from sterile soils were much slower with recoveries of over 50% after 20 weeks. incubation of soil with avadex t ...	1976	1267481
[formation of beta-fructofuranosidase by penicillium vitale pidopl. et bilai and other species of the genus penicillium lk].		2006	1219324
[variability in penicillium janthinellum biourge, a producer of the antibiotic janthinellin, under the action of nitrosomethylurea].	variation of the janthinellin-producing organism p. janthinellum was induced by nitrozomethylurea (nmu). the following concentrations of nmu were tested: 0.5; 0.25, 0.125 per cent at the exposure time of 15 minutes, 1 and 10 hours. the conidia survival had back dependence on the concentration and exposure time. the morphological variation was evident from the presence of forms with changed colour of the colony surface mycelium, light green, brown, light yellow, yellow-pink and white conidia and ...	1975	1211902
kininase and anti-inflammatory activities of acid carboxypeptidase from penicillium janthinellum.	the acid carboxypeptidase from penicillium janthinellum catalyzed the rapid release of arginine, and the slow release of phenylalanine, proline, serine and glycine from the carboxy-terminal of bradykinin at ph 4.15 to 4.8. anti-inflammatory activity of the acid carboxypeptidase seems to suggest that the enzyme hydrolyzed bradykinin in vivo.	1975	1204716
[preparation in crystalline form of catalase from penicillium vitale pidopl. et bilai].		1975	1204470
[method of purification of glucose oxidase by means of affinity chromatography on immunoabsorbent].	the possibility to purify glucose oxidase from penicillium vitale on immunosorbent containing specific antibodies to the enzyme covalently bound with sepharose 4b is studied. the method of affinity chromatography was applied, beside routine methods of fractionating blood serum proteins, to isolate specific antibodies from antiserum of rabbits immunized with glucose oxidase. immobilized on sepharose glucose oxidase was used as biospecific sorbent. specific antibodies to the enzyme were isolated u ...	1975	1203397
[comparative evaluation of the enzymatic activity of the mycelial homogenates from penicillium vitale pidopl. et bilai broken down by different methods].		2006	994870
[natural variability of penicillium vitale pidopl. et bilai and characteristics of active variants of the fungus that produce glucose oxidase].		2008	916914
[stabilizing effect of calcium ions on glucose oxidase of penicillium vitale].	reconstruction of p. vitale glucose oxidase from apeonzyme and coenzyme (fad) was studied as affected by iodine acetate as well as mercury and calcium ions. mercury ions, iodine acetate as well as the labilizing fraction (flavin adenine-containing component) are established to inhibit the reconstruction affecting the sulphydryl groups of the apoenzyme which take part in addition of fad to it. calcium ions prevent the effect of the "labilizing" fraction, iodine acetate and mercury ions on the glu ...	2013	888220
[mechanism of labilization of penicillium vitale glucose oxidase].	the article deals with conditions for splitting the penicillium vitale glucosooxidase molecule into apoenzyme and coenzyme, for reconstruction of the enzyme from apoenzyme and fad as well as for the effect of some factors ("labilizing" fraction, dithiotreitol) on it. specific activity of the reconstructed enzyme is on the average 85% of the initial enzyme specific activity: "the labilizing" fraction inhibits reconstruction of glucosooxidase from apoenzyme and fad, that is due to oxidation of the ...	2008	867542
purification & properties of an extracellular dextranase from penicillium janthinellum.		1978	748164
[spectral properties of the prosthetic group of penicillium vitale catalase].	differences in the absorption spectrum of the penicillum vitale catalase in the visible region as compared to the absorption spectrum for catalase of animal origin are established to be due to the prosthetic group of the enzyme. a molecule of p. vitale catalase is determined to contain 0.051 +/- 0.0003% of iron. it corresponds to two iron atoms per enzyme molecule and to a twice as low content of iron as in a molecule of the bovine liver catalase. an assumption is advanced that the p. vitale cat ...	1978	664035
[stabilization of modified glucose oxidase from penicillium vitale incorporated into the polymeric chains of gels].	glucose oxidase from penicillium vitale was modified by unsaturated compounds, e.g. acrolein, allylisothiocyanate, acryloyl chloride and maleic anhydride. the degree of modification for the respective agents made up to 27.2, 8.3, 11.1 and 35.0% of the amine residues; the enzymatic activity was thereby retained by 98, 100, 0.03 and 58%, respectively. the thermal stability of modified enzymes was decreased 2-7 times. the modified preparations were copolymerized with acrylamide or 2-hydroxyethyl me ...	1978	656485
[ultrastructure of penicillium vitale pidopl. et bilai--producer of catalase and glucose oxidase].		2006	604723
[effect of amino acids on transformation of glucose oxidase as antigen in tissues of immunized animals].	the effect of certain amino acids on transformation of glucoseoxidase as an antigen in different tissues of the animals immunized with it showed that the used glucoseoxidase of the fungus penicillium vitale pidopl. et bilaj possesses antigenic properties peculiar to this enzyme isolated from other sources. 1.5 minutes after a single administration of the antigen to nonimmunized rabbits it is determined in them in descending amounts in such an order: blood, lungs, liver, kidneys, lymphatic nodes. ...	1978	567396
the effects of temperature on growth of four high arctic soil fungi in a three-phase system.	the effect of temperature on the growth of chrysosporium pannorum, cylindrocarpon sp., penicillium janthinellum, and phoma herbarum, isolated from tundra soils, was studied. the growth in two systems, glucose-mineral agar plates and sand, moistened with glucose-mineral broth, was compared. all isolates showed an exponential increase in mass (measured as protein increase) in sand and a linear rate of extension on agar. radial increase on agar was shown not to be a good index of growth in sand. tr ...	1978	565246
production of tremorgenic toxins by penicillium janthinellum biourge: a possible aetiological factor in ryegrass staggers.	topsoil, herbage and faeces collected during an outbreak of ryegrass staggers in sheep were examined for tremorgenic penicillia. no such fungi were recovered from the plant material, but they were found among the predominant fungi in the soil and faecal samples. the commonest species of penicillium, and almost the only tremorgenic species encountered, was penicillium janthinellum biourge. when fed to sheep, the mycelium of this fungus evoked a number of the clinical signs seen in field cases of ...	1979	475667
the primary structure of calf chymosin.	the complete amino acid sequence of calf chymosin (rennin) (ec 3.4.23.4) has been determined. the sequence consists of a single peptide chain of 323 amino acid residues. the primary structure of the precursor part of calf prochymosin was published previously (pedersen, v.b., and foltmann, b. (1975) eur. j. biochem. 55, 95-103), thus we are now able to account for the total 365 amino acid residues of calf prochymosin. comparison of the sequence of calf prochymosin with that of pig pepsinogen a (e ...	1979	381305
penicillopepsin: 2.8 a structure, active site conformation and mechanistic implications.	the crystal structure of penicillopepsin, an extracellular acid protease isolated from the mold penicillium janthinellum, has been determined at 2.8 a resolution by the method of multiple isomorphous replacement. the resulting electron density map computed from the native structure factor amplitudes and mir phases has an overall mean figure of merit of 0.90. the molecule is decidedly nonspherical, with the majority of residues in beta-structure. there is an 18-stranded mixed beta-sheet which for ...	1977	339694
penicillopepsin from penicillium janthinellum crystal structure at 2.8 a and sequence homology with porcine pepsin.	the polypeptide chain of the acid protease penicillo pepsin folds via an 18-stranded mixed beta-sheet into two distinct lobes separated by a 30-a long groove which is the extended substrate binding site. the catalytic residues asp-32 and asp-215 are located in this groove and their carboxyl groups are in intimate contact. alignment of the amino acid sequence with that of pepsin shows regions of high homology.	1977	323722
action of crystalline acid carboxypeptidase from penicillium janthinellum.	acid carboxypeptidase (ec 3.4.12.-) crystallized from culture filtrate of penicillium janthinellum has been investigated for its use in carboxy-terminal sequence determination of z-gly-pro-leu-gly, z-gly-pro-leu-gly-pro, angiotensin i, native lysozyme, native ribonuclease t1, and reduced s-carboxy-methyl-lysozyme. the examination indicated that proline and glycine were liberated from z-gly-pro-leu-gly-pro. at high enzyme concentration, the enzyme catalyzed complete sequential release of amino ac ...	1975	239751
[immobilization of penicillium vitale glucose-oxidase on aminosilochrome and properties of immobilized enzyme].	penicillium vitale glucose-oxidase modified by means of the carbohydrate component oxidation is added covalently to aminoorganosylochrome. the activity of the immobilized preparations is 20-38% depending on the protein-carrier ration in immobilization. comparison of some properties of native and immobilized glucose-oxidase showed that the rh optimum of the immobilized glucose-oxidase is slightly widened towards the alkaline regions; the immobilized glucose-oxidase possesses a considerably higher ...	2015	38549
[total activity of glucose oxidase, catalase and invertase and their distribution in the mycelial subcellular fractions of penicillium vitale pidopl. et bilai].		2006	32465
kinetics and stability of glucooxidase from penicillium vitale.	the kinetics, thermal stability and ultrasonic resistance of glucosoxidase from penicillium vitale have been investigated. its oxidative constant is close to, catalytic constant is 1.7 times less and the reductive constant is 5.4 times more than the respective constants of the enzyme from aspergillus niger. the relationship between the thermal inactivation constant (k(i)=2.1 pt 15.3. the same relationship is true of the ultrasonic inactivation constant and ph in the ph range of 6.5-8.0. in a mor ...	1978	27781
[dissociation of penicillium vitale catalase under the effect of urea and acid ph].	conditions are studied for penicillium vitale catalase dissociation into subunits under the effect of urea (0.7-8.5 m) and acid ph (5.0-2.0). in 8.0 m urea (ph 5.0) a molecule of the p. vitale catalase dissociates with formation of the components, the sedimentation coefficient of which is 2.4+/-0.2s, the molecular weight is 153000+/-2800. dissociation at ph 2.2 in 4.0 m urea results in formation of components with the sedimentation coefficient 2.2+/-0.3 s. the catalase molecule dissociation unde ...	2013	18833
[industrial experiment in obtaining catalase from penicillium vitale pidopl. et bilai].		2006	11395
[comparative characteristics of catalase from the fungus penicillium vitale, which is synthesized under different nutritional conditions].	a comparative study of properties (absorption spectra, thermostability, ph optimum, polyacrylamide gel electrophoresis, deae-cellulose separation) and structure (amino acid composition, finger-prints, carbohydrate composition) was performed for p. vitale catalase synthesized under different medium conditions. in all cases the results were similar. the only difference occured in the amount of synthesized proteins. a conclusion is drawn that under different nourishing conditions of the fungus the ...	2006	3004