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antifungal and antioxidative potential of oil and extracts derived from leaves of indian spice plant cinnamomum tamala.plant—based antimicrobials and antioxidants represent a vast untapped source for medicines and food supplements and hence have enormous therapeutic potential. present work reports the fungicidal potential of cinnamomum tamala nees & eberm (lauraceae) leaf oil against five food spoilage and pathogenic fungi. in addition antioxidant efficacy of seven different solvent extracts derived from leaf was also evaluated using in vitro models. the oil demonstrated potent antifungal activity against asperg ...201223273204
synthesis of some potent immunomodulatory and anti-inflammatory metabolites by fungal transformation of anabolic steroid oxymetholone.biotransformation of organic compounds by using microbial whole cells provides an efficient approach to obtain novel analogues which are often difficult to synthesize chemically. in this manuscript, we report for the first time the microbial transformation of a synthetic anabolic steroidal drug, oxymetholone, by fungal cell cultures.201223237028
production, statistical optimization and application of endoglucanase from rhizopus stolonifer utilizing coffee husk.coffee cherry husk (ch) is one of the major by-products obtained from coffee processing industry and accounts to 43 ± 5.9% of cellulose. screening of fungal organism for cellulase production was carried out and the potential organism was identified as rhizopus stolonifer by internal transcribed spacer's (its)-5.8s rdna analysis. a systematic study with response surface methodology (rsm) based on ccrd was used to study the interactions among the variables such as ph (3-7), moisture (40-80%) and p ...201323223909
broad-spectrum antifungal-producing lactic acid bacteria and their application in fruit models.a large-scale screen of some 7,000 presumptive lactic acid bacteria (lab), isolated from animal, human, or plant origin, identified 1,149 isolates with inhibitory activity against the food-spoilage mould penicillium expansum. in excess of 500 lab isolates were subsequently identified to produce a broad spectrum of activity against p. expansum, penicillium digitatum, penicillium notatum, penicillium roqueforti, rhizopus stolonifer, fusarium culmorum, aspergillus fumigatus and rhodotorula mucilagi ...201323160868
generation of the volatile spiroketals conophthorin and chalcogran by fungal spores on polyunsaturated fatty acids common to almonds and pistachios.the spiroketal (e)-conophthorin has recently been reported as a semiochemical of the navel orangeworm moth, a major insect pest of california pistachios and almonds. conophthorin and the isomeric spiroketal chalcogran are most commonly known as semiochemicals of several scolytid beetles. conophthorin is both an insect- and plant-produced semiochemical widely recognized as a nonhost plant volatile from the bark of several angiosperm species. chalcogran is the principal aggregation pheromone compo ...201223153034
bacillus cereus ar156 induces resistance against rhizopus rot through priming of defense responses in peach fruit.the biocontrol effects of bacillus cereus ar156 on rhizopus rot caused by rhizopus stolonifer in postharvest peach fruit and the possible mechanisms were investigated. the results showed that fruit treated with b. cereus ar156 had significantly lower disease incidence and smaller lesion diameter than the control fruit did. b. cereus ar156 treatment remarkably enhanced activities of chitinase and β-1,3-glucanase, promoted accumulation of h(2)o(2), and improved total phenolic content and 2,2-diphe ...201323122077
heterologous expression and secretion of an antifungal bacillus subtilis chitosanase (csnv26) in escherichia coli.the aims of the study were the production improvement, the purification, the characterization and the activity investigation of chitosanase csnv26 of bacillus subtilis (v26). the gene csnv26 encoding for this protein was amplified and cloned in the pbad vector then expressed in escherichia coli (top10). the sds-page and zymogram analysis of the recombinant protein showed that it has two active forms sized 27 and 31 kda, corresponding to the protein with and without signal peptide. this protein h ...201323065029
the mitochondrial respiratory chain of rhizopus stolonifer (ehrenb.:fr.) vuill.rhizopus stolonifer (ehrenb.:fr.) vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes i, ii, iii, and iv) and the f(1)f(0)-atp synthase (complex v). using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. the composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organi ...201323064442
efficacy of the application of a coating composed of chitosan and origanum vulgare l. essential oil to control rhizopus stolonifer and aspergillus niger in grapes (vitis labrusca l.).this study evaluated the efficacy of the combined application of chitosan (chi) and origanum vulgare l. essential oil (ov) in the inhibition of rhizopus stolonifer urm 3728 and aspergillus niger urm 5842 on laboratory media and on grapes (vitis labrusca l.) and its influence on the physical, physicochemical and sensory characteristics of the fruits during storage (25 °c, 12 days and 12 °c, 24 days). the application of mixtures of different chi and ov concentrations (minimum inhibitory concentrat ...201222986200
[harmful biological agents at museum workposts].the aim of the studies was to determine the level and kind of microbiological contamination of air and surfaces in museum premises with various collection specificities. in addition, the criteria for selecting indicators of contamination with harmful biological agents at museum workposts are proposed.201222779322
zygomycota associated with traditional meju, a fermented soybean starting material for soy sauce and soybean paste.various zygomycota species were detected during a study of the mycobiota of meju, a brick of dried fermented soybeans, used in korean cuisine. two hundred and sixty-eight strains were isolated from 98 finished meju products collected in various regions of korea from 2009 to 2011, and 96 strains were isolated from in-process meju on various farms from 2010 to 2011. the isolated zygomycota were identified using phenotypic characteristics combined with dna sequences of the internal transcribed spac ...201222752901
occupational exposure to airborne fungi in two croatian sawmills and atopy in exposed workers.airborne fungi were collected over a one year period at 2-month intervals at 2 sawmills in croatia (sm 1 and sm 2) processing mainly beech wood and oak wood. a questionnaire concerning respiratory symptoms and skin prick test (spt) with common inhalatory allergens and moulds cladosporium herbarum, alternaria alternata, aspergillus niger, penicillium notatum, and rhizopus nigricans were performed in 96 workers from the same sawmills. average concentrations of airborne fungi were 1,696-7,316 cfu/m ...201222742790
mycobiota and mycotoxins (aflatoxins and ochratoxin) associated with some saudi date palm fruits.this study aimed to determine the mycological profile of the retail date fruits distributed in different markets at taif, saudi arabia. the presence of aflatoxins and ochratoxin a was also measured. twenty-two fungal species belonging to 12 genera were isolated from 50 different date samples. aspergillus flavus, a. niger, penicillium chrysogenum, and rhizopus stolonifer were the most prevalent species among isolated fungi. eighty isolates of a. flavus and 36 of a. niger were detected for their a ...201222568750
antimicrobial activity of saponin-rich fraction from camellia oleifera cake and its effect on cell viability of mouse macrophage raw 264.7.as a by-product of oil production, several million tons of camellia oleifera cake is discarded every year in china. the aim of this study was to evaluate the antimicrobial activity of a saponin-rich fraction isolated from c. oleifera cake and investigate its effect on the cell viability of raw 264.7, a macrophage-like cell line present in almost all mouse tissues.201222430639
antagonistic action of bacillus subtilis strain fmbj on the postharvest pathogen rhizopus stolonifer.the antagonistic activities of bacillus subtilis fmbj against the rhizopus stolonifer pathogen causing peach soft rot disease were studied in this paper. bacillus subtilis fmbj exhibited a high antifungal effect on the mycelium growth, sportulation, and germ tube elongation of r. stolonifer with the inhibition rate of 86.5% and 97.42%, respectively, spore germination rate of 20% under antagonist strain concentration of 10⁸ cfu/ml. by using of scanning electron microscope (sem) and transmission e ...201122417435
in vitro control of post-harvest fruit rot fungi by some plant essential oil components.eight substances that are main components of the essential oils from three mediterranean aromatic plants (verbena officinalis, thymus vulgaris and origanum vulgare), previously found active against some phytopathogenic fungi and stramenopila, have been tested in vitro against five etiological agents of post-harvest fruit decay, botrytis cinerea, penicillium italicum, p. expansum, phytophthora citrophthora and rhizopus stolonifer. the tested compounds were β-fellandrene, β-pinene, camphene, carva ...201222408454
antifungal activity in ethanolic extracts of carica papaya l. cv. maradol leaves and seeds.bioactive compounds from vegetal sources are a potential source of natural antifungic. an ethanol extraction was used to obtain bioactive compounds from carica papaya l. cv. maradol leaves and seeds of discarded ripe and unripe fruit. both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. the effect of time on extraction efficiency was confirmed by qualitative identif ...201122282629
influence of fungal elicitation on glycyrrhizin production in transformed cell cultures of abrus precatorius linn.glycyrrhizin, obtained from abrus precatorius (indian liquorice), is a phytoconstituent of importance for pharmaceutical and food industries.201122262933
novel copper-based therapeutic agent for anti-inflammatory: synthesis, characterization, and biochemical activities of copper(ii) complexes of hydroxyflavone schiff bases.four hydroxyflavone derivatives have been synthesized with the aim of obtaining a good model of superoxide dismutase. better to mimic the natural metalloenzyme, copper complexes have been designed. the cu(ii) complexes of general formulae, [cul] where l = 5-hydroxyflavone-o-phenylenediamine (l¹h₂)/m-phenylenediamine (l²h₂) and 3-hydroxyflavone-o-phenylenediamine (l³h₂)/m-phenylenediamine (l⁴h₂) have been synthesized. the structural features have been determined from their analytical and spectral ...201222238015
antifungal activity of essential oil from asteriscus graveolens against postharvest phytopathogenic fungi in apples.the essential oils of the aerial parts of asteriscus graveolens have been studied using gc and gc-ms. twenty-eight compounds were identified in the essential oil amounting to 94.9% of the total oil. the aerial part oils showed similar chromatographic profiles and were characterized by having a high content of oxygenated sesquiterpenes with 6-oxocyclonerolidol (66.7% +/- 5.5) and 6-hydroxycyclonerolidol (8.8% +/-1.2) as the major components. the antifungal effect of the essential oil from a. grav ...201122224306
time-course changes in fungal elicitor-induced lignan synthesis and expression of the relevant genes in cell cultures of linum album.linum album has been shown to accumulate anti-tumor podophyllotoxin (ptox) and its related lignans. in the present study, we examined the effects of five fungal extracts on the production of lignans in l. album cell cultures. fusarium graminearum extract induced the highest increase of ptox [140μgg(-1) dry weight (dw) of the l. album cell culture] which is seven-fold greater than the untreated control, while rhizopus stolonifer extract enhanced the accumulation of lariciresinol, instead of ptox, ...201222218086
steroid 11ß-hydroxylation by a fungal microsomal cytochrome p450.the steroid 11ß-hydroxylase activity of the fungus cochliobolus lunatus was increased about 100-fold by cultivation of mycelia for 4-5 h with 20-hydroxymethyl-1,4-pregnadien-3-one. cell-free extracts revealed a maximum activity of 45 nmol 11ß-hydroxyprogesterone/h·mg protein in the 100,000 g pellet fraction. the 11ß-hydroxylation was dependent on nadph. the formation of 11ß-hydroxyprogesterone correlated linearly with the cytochrome p450 concentration. the fungal 11ß-hydroxylase transformed both ...199222217857
Essential oil from Ocimum basilicum (Omani Basil): a desert crop.The focus of the present study was on the influence of season on yield, chemical composition, antioxidant and antifungal activities of Omani basil (Ocimum basilicum) oil. The present study involved only one of the eight Omani basil varieties. The hydro-distilled essential oil yields were computed to be 0.1%, 0.3% and 0.1% in the winter, spring and summer seasons, respectively. The major components identified were L- linalool (26.5-56.3%), geraniol (12.1-16.5%), 1,8-cineole (2.5-15.1%), p-allylan ...201122164790
glucosinolate profiling and antimicrobial screening of aurinia leucadea (brassicaceae).glucosinolates (gls) were characterized in various aerial parts (stems, leaves, and flowers) of aurinia leucadea (guss.) c. koch and quantified according to the iso 9167-1 official method based on the hplc analysis of desulfoglucosinolates. eight gls, i.e., glucoraphanin (gra), glucoalyssin (gal; 1), gluconapin (gna; 2), glucocochlearin (gcc), glucobrassicanapin (gbn; 3), glucotropaeolin (gtl), glucoerucin (ger), and glucoberteroin (gbe) were identified. the total gl contents were 57.1, 37.8, an ...201122162169
A new antifungal coumarin from Clausena excavata.A new ?-lactone coumarin, named as excavarin-A, showing antifungal activity was isolated from the leaves of Clausena excavata by bioassay guided fractionation method. The structure was elucidated by spectroscopic data analysis and identified as 7((2E)-4(4,5-dihydro-3-methylene-2-oxo-5-furanyl)-3-methylbut-2-enyloxy) coumarin. Minimum inhibitory concentration (MIC) was determined against fifteen fungal strains pathogenic against plants and human. The least MIC was recorded against the human patho ...201222088496
isolation and identification of microbes associated with mobile phones in dammam in eastern saudi arabia.this study was conducted to determine microbial contamination of mobile phones in the city of dammam, in the eastern region of saudi arabia, and identify the most important microbial species associated with these phones in order to take the necessary remedial measures.201022022665
antimicrobial, antiplasmodial, haemolytic and antioxidant activities of crude extracts from three selected togolese medicinal plants.to investigate the antioxidant, antimicrobial, antiplasmodial, acute toxicity and haemolytic activities of methanolic extracts of three plants. phytochemical analysis to determine the phenolic contents was also carried out.201122014738
reduction of nectarine decay caused by rhizopus stolonifer, botrytis cinerea and penicillium digitatum with aloe vera gel alone or with the addition of thymol.two nectarine cultivars ('flavela' and 'flanoba') were treated with aloe vera gel alone, or with the addition of thymol, and then inoculated with rhizopus stolonifer, botrytis cinerea and penicillium digitatum. both treatments were effective in reducing the decay incidence caused by the 3 fungi species, although the addition of thymol did not generally improve the efficacy of aloe vera gel on reducing the infection damage. the coatings were clearly effective in reducing the postharvest ripening ...201121974979
The antifungal, cytotoxic, antitermite and insecticidal activities of Zizyphus jujube.Plants are very useful, self-generating machines, producing a variety of useful bioactive products. Keeping in view this idea, the crude methanolic extract and various fractions of Zizyphus jujuba were screened for antifungal, cytotoxic, antitermite and insecticidal activities. Low activity was shown by the crude methanolic extract (12%), n-hexane (9%), chloroform (20%) and ethyl acetate (14%) fraction against Penicillium notatum. Low activity was shown by the n-hexane fraction against Aspergill ...201121959810
Liriodenine, early antimicrobial defence in Annona diversifolia.Annonaceae aporphine alkaloids, of which liriodenine is the most abundant, have not been extensively studied from a biological standpoint. The goal of this study was to investigate the role of liriodenine in antimicrobial defense during early developmental stages in Annona diversifolia. The fungi Rhizopus stolonifer and Aspergillus glaucus, which are responsible for seed deterioration, were isolated during imbibition, and their antifungal activity was determined by diffusion, macrodilution, and ...201121950162
11a-Hydroxylation of 16a,17-epoxyprogesterone by Rhizopus nigricans in a biphasic ionic liquid aqueous system.11a-Hydroxylation of 16a,17-epoxyprogesterone (EP) by Rhizopus nigricans is an essential step in the synthesis of many steroidal drugs, while low conversion of the biohydroxylation is a tough problem to be solved urgently in industry. Two ionic liquids (ILs) of [BMIm][PF(6)] and [BMIm][NTf(2)] were used in the biotransformation of EP by R. nigricans. The results indicated that the conversion carried out in [BMIm][PF(6)]-aqueous biphasic system was greatly increased to above 90% at 18 g/L feeding ...201121855322
biotransformation of (20s)-20-hydroxymethylpregna-1,4-dien-3-one by four filamentous fungi.microbial transformation of (20s)-20-hydroxymethylpregna-1,4-dien-3-one (1) by four filamentous fungi, cunninghamella elegans, macrophomina phaseolina, rhizopus stolonifer, and gibberella fujikuroi, afforded nine new, and two known metabolites 2-12. the structures of these metabolites were characterized through detailed spectroscopic analysis. these metabolites were obtained as a result of biohydroxylation of 1 at c-6ß, -7ß, -11a, -14a, -15ß, -16ß, and -17a positions, except metabolite 2 which c ...201121762714
microbial transformations of (+)-isomenthol by fusarium lini and rhizopus stolonifer.microbial transformation of (+)-isomenthol (1) by various strains of fungi was investigated. fusarium lini has successfully converted compound 1 into a new metabolite, 5+¦-hydroxyisomenthol (2), and a known metabolite, 1+¦-hydroxyisomenthol (3), whereas incubation with rhizopus stolonifer only yielded metabolite 3. the transformed metabolites were structurally characterized on the basis of their spectral data.201121720039
the response of filamentous fungus rhizopus nigricans to flavonoids.the saprophytic fungus rhizopus nigricans constitutes a serious problem when thriving on gathered crops. the identification of any compounds, especially natural ones, that inhibit fungal growth, may therefore be important. during its life cycle, rhizopus nigricans encounters many compounds, among them the flavonoids, plant secondary metabolites that are involved in plant defense against pathogenic microorganisms. although not being a plant pathogen, rhizopus nigricans may interact with these com ...201121656793
natural resistance of rose petals to microbial attack.petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ...201121614712
molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from rhizopus stolonifer strain yf6 which can accumulate high levels of gamma-linolenic acid.the delta 6-desaturase gene was cloned from rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (gla, c18:3 ?(6,9,12)) to the total fatty acids. the cloned dna contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and hpgg motif. notably, this deduced sequence had a shorter c-terminus. results demonstrated that the cdna sequence exhibite ...201121369993
antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against rhizopus stolonifer.the antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against rhizopus stolonifer were investigated. the mic (minimal inhibitory concentration) of fengycin without commercial surfactin added was 0.4 mg/ml while the mic of fengycin with commercial surfactin added was 2.0 mg/ml. fengycin acted on cell membrane and cellular organs and inhibited dna synthesis. the antifungal effect of fengycin was reduced after commercial surfactin was added. all these ...201121369992
screening filamentous tropical fungi for their nutritional potential as sources of crude proteins, lipids and minerals.the use of fungal and yeast biomass in foodstuff, either as supplements or as major ingredients in formulations is an area of growing interest for the modern food industry. the aim of this study was to evaluate the nutritional potential of biomasses obtained from filamentous fungi penicillium sclerotiorum, penicillium janthinellum, rhizopus stolonifer and syncephalastrum racemosum. biomasses presented 26-37% of total proteins, 1.7-3.5% of lipids and 4.6-9.1% of ashes. the humidity level reached ...201021339148
interactions between various microbes and ginseng botanicals.three kinds of interactions occur between ginseng botanicals and microorganisms: a) spoilage of the botanical by various fungi (e.g., aspergillus, penicillium, alternaria, and eurotium species) and bacteria; b) transformation of ginsenosides into more bioactive forms by bacteria such as intrasporangium sp. gs603, microbacterium sp. gs514, caulobacter leidyia, bifidobacterium sp. int57, bifidobacterium sp. sj32, fusobacterium sp. and bacteroides sp., and moulds (e.g., aspergillus niger, fusarium ...201121254831
identification and partial characterization of rhizopus nigricans gβ proteins and their expression in the presence of progesterone.the mammalian steroid hormone progesterone actuates a signalling pathway in the zygomycete rhizopus nigricans which includes heterotrimeric g proteins. to investigate the possibility that the gβ subunit of these proteins is involved in the signalling, a cdna library from r. nigricans exposed to progesterone was prepared and a sequence coding for a gβ subunit was searched for. using degenerate primers, two sequences, rngpb1 and rngpb2, were identified that exhibited a high degree of identity with ...201021195176
influence of growth regulators and elicitors on cell growth and α-tocopherol and pigment productions in cell cultures of carthamus tinctorius l.the present study examined the effects of plant growth hormones, incubation period, biotic (trametes versicolor, mucor sp., penicillium notatum, rhizopus stolonifer, and fusarium oxysporum) and abiotic (nacl, mgso(4), feso(4), znso(4), and fecl(3)) elicitors on cell growth and α-tocopherol and pigment (red and yellow) productions in carthamus tinctorius cell cultures. the cell growth and α-tocopherol and pigment contents improved significantly on murashige and skoog (ms) liquid medium containing ...201021120469
an attempt of postharvest orange fruit rot control using essential oils from mediterranean plants.twelve essential oils from mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: botrytis cinerea, penicillium italicum, phytophthora citrophthora, and rhizopus stolonifer. essential oils were obtained from hyssopus officinalis, lavandula angustifolia, majorana hortensis, melissa officinalis, ocimum basilicum, origanum vulgare, salvia officinalis, and thymus vulgaris (family lamiaceae), verbena officinalis (family ...201020874226
ntkti1, a kunitz trypsin inhibitor with antifungal activity from nicotiana tabacum, plays an important role in tobacco's defense response.a cdna library from tobacco inoculated with rhizoctonia solani was constructed, and several cdna fragments were identified by differential hybridization screening. one cdna clone that was dramatically repressed, ntkti1, was confirmed as a member of the kunitz plant proteinase inhibitor family. rt-pcr analysis revealed that ntkti1 was constitutively expressed throughout the whole plant and preferentially expressed in the roots and stems. furthermore, rt-pcr analysis showed that ntkti1 expression ...201020735473
biocrystallization of silver and gold ions by inactive cell filtrate of rhizopus stolonifer.microbes and their cell filtrates are known to synthesize metal nanoparticles. but maintenance of aseptic conditions and irregularly shaped and sized nanoparticles are major drawbacks of the system. in this study cell filtrate from inactive biomass of rhizopus stolonifer was used for the first time to produce near uniformly sized and shaped ag and au nanoparticles at room temperature. the size of ag and au nanoparticles were found in the range of 25-30 nm and 1-5 nm, respectively. uv-vis spectru ...201020627484
[7alpha-hydroxylation of steroid 5-olefins by mold fungi].hydroxylation activity of the mold fungi belonging to the orders dothideales, hypocreales, and mucorales towards delta(5)-3beta-hydroxysteroids was studied. the fungi bipolaris sorokiniana, fusarium sp., and rhizopus nigricans were able to introduce hydroxy group at position 7alpha; however, this ability was detected only at a low substrate load and with a low yield. a 7alpha-hydroxylase activity of the curvularia lunata vkpm f-981 culture was shown for the first time. it was demonstrated that t ...201020198922
a combination of heat treatment and pichia guilliermondii prevents cherry tomato spoilage by fungi.this study investigated the effectiveness of heat treatment and pichia guilliermondii, either alone or in combination, to combat postharvest fungal spoilage in cherry tomato fruit. in vitro experiments demonstrated that heat treatment at 38 degrees c significantly inhibited mycelial growth of three different pathogens (botrytis cinerea, alternaria alternata and rhizopus stolonifer ehrenb). in vivo experiments unveiled that either heat treatment or p. guilliermondii reduced decay caused by these ...201019923029
molecular characterization of mucor circinelloides and rhizopus stolonifer strains isolated from some saudi fruits.thirteen strains of mucor circinelloides and nine strains of rhizopus stolonifer were collected from different saudi fruits. also, two strains of m. circinelloides and one strain of r. stolonifer isolated from soil were used in this study to analyze the genetic diversity among these 25 strains. the dendrogram constructed from random amplified polymorphic dna-polymerase chain reaction results using two primers (v6 and m13) showed no correlation between clustering system and sources of isolation f ...201019877768
isoelectric points for the mycelium of fungi.1. mycelium of rhizopus nigricans when stained with certain acid and basic dyes and washed with buffer mixtures of 0.1 m phosphoric acid and sodium hydroxide responded much like an amphoteric colloid with an isoelectric point near ph 5.0. 2. when grown on potato dextrose agar the reaction of which was varied with phosphoric acid the extent of colony growth of rhizopus nigricans plotted against the initial sörensen value of the agar produced a double maximum curve with the minimum between the two ...192419872067
a new selective medium for the recovery and enumeration of monilinia fructicola, m. fructigena, and m. laxa from stone fruits.isolation of monilinia spp. from stone and pome fruit surfaces is difficult due to the presence of several fast-growing fungal species such as rhizopus, alternaria, and penicillium spp. therefore, a new selective medium (acidified potato dextrose agar [ph 3.6] amended with fosetyl-aluminum [fosetyl-al] at 500 microg/ml) (apda-f500) was developed for the recovery of monilinia propagules. the antifungal agents fosetyl-al, dichloran, ammonium molybdate, and 2-deoxy-d-glucose (2-dd-glucose) were tes ...200919740034
new oxandrolone derivatives by biotransformation using rhizopus stolonifer.structural transformation of the steroidal lactone, oxandrolone (1), by suspended-cell cultures of the plant pathogen fungus rhizopus stolonifer, resulted in the production of three new metabolites. these metabolites were identified as 11alpha-hydroxyoxandrolone (2), 6alpha-hydroxyoxandrolone (3) and 9alpha-hydroxyoxandrolone (4), by different spectroscopic methods and single-crystal x-ray diffraction analysis for metabolite 2. compounds 1 and 3 showed a significant beta-glucuronidase inhibitory ...200919698730
cloning and expression of cellulase gene eg1 from rhizopus stolonifer var. reflexus tp-02 in escherichia coli.a novel gene, eg encoding enzymes involved in carboxymethyl cellulose (cmc) degradation was isolated, sequenced from the filamentous fungus rhizopus stolonifer var. reflexus tp-02, and expressed in escherichia coli bl21. the results showed that the gene amplified from the cdna of the strain could be classified as the family of endoglucanase. during the fermentation process, the maximum endoglucanase activity (i.e. 0.715 iu/ml) of the recombinant bacteria was obtained at 36 h. the sds-page analys ...200919640700
heat treatment in combination with antagonistic yeast reduces diseases and elicits the active defense responses in harvested cherry tomato fruit.this study investigated the effects of heat treatment (hot air at 38 degrees c) and antagonistic yeast (pichia guilliermondii) alone or in combination against postharvest diseases (botrytis cinerea, alternaria alternata and rhizopus nigricans) on cherry tomato fruit, and evaluated the elicitation of active defense responses. results showed that heat treatment at 38 degrees c for 24 h in combination with p. guilliermondii at 1 x 10(8) cfu ml(-1) was the most effective approach to reduce various i ...200919637930
sporicidal activity of synthetic antifungal undecapeptides and control of penicillium rot of apples.the antifungal activity of cecropin a(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. a set of 15 sequences was screened in vitro against fusarium oxysporum, penicillium expansum, aspergillus niger, and rhizopus stolonifer. most compounds were highly active against f. oxysporum (mic < 2.5 microm) but were less active against the other fungi. the best peptides were studied for their sporicidal activity and for sytox green upt ...200919617390
rapid detection and quantification of aspergillus fumigatus in environmental air samples using solid-phase cytometry.aspergillus fumigatus is an ubiquitous fungus capable of causing severe infections such as aspergilloma, allergic bronchopulmonary aspergillosis, and invasive aspergillosis, especially in immunocompromised patients. monitoring the number of aspergillus fumigatus spores in the air is crucial for infection control. in the present study, a novel approach for the quantification of aspergillus fumigatus, based on solid-phase cytometry (spc) and immunofluorescent labeling, was developed. the sensitivi ...200919534140
evaluation of food additives as alternative or complementary chemicals to conventional fungicides for the control of major postharvest diseases of stone fruit.to evaluate potential alternatives to conventional fungicides to control decay, more than 20 food additives and generally regarded as safe compounds were tested at three concentrations in in vivo primary screenings with several cultivars of california peaches, nectarines, and plums that had been artificially inoculated with seven major postharvest pathogens: monilinia fructicola, botrytis cinerea, geotrichum candidum, alternaria alternata, penicillium expansum, mucor piriformis, and rhizopus sto ...200919517732
[relationship of zn content and na/k ratio to the resistance to pathogen invasion in antisense acs transgenic tomato fruit and normal tomato].in the present work, inductively coupled plasma atomic emission spectrometry (icp-aes) was used to determine the content of zn, na and k and the ratio of na/k in antisense acs transgenic tomato fruit (in which the antisense acc synthase gene construct was inserted) and in normal tomato fruit of the same variety (lycopersicon esculentum cv. lichun). the difference in mineral elements content between the two tomatoes and the involvement of zn content and na/k ratio in the disease resistance of pos ...200919445235
identification and characterization of a novel delta6-fatty acid desaturase gene from rhizopus nigricans.fatty acid composition of fungi is analysed through the gas chromatography technique. with specific activity a novel enzyme delta6-fatty acid desaturase was screened and isolated from rhizopus nigricans. in this study r. nigricans was identified as a fungal species that produced plentiful gamma-linolenic acid. a 1,475 bp full-length cdna, designated as rnd6d here, with high homology to fungal delta6-fatty acid desaturase genes was isolated from r. nigricans using reverse transcription polymerase ...200919152021
microbial transformation of testosterone by rhizopus stolonifer and fusarium lini.the microbial transformation of testosterone by the fungi rhizopus stolonifer and fusarium lini has been investigated for the first time. the bioconversion reactions observed from r. stolonifer included oxidation of a 17beta-hydroxyl group, a hydroxylation at equatorial 11alpha position, reduction of the double bond at c-4 with oxidation of the methylene at c-6 to the corresponding keto group, and lactonisation of ring d; the latter is the first report of this reaction by a rhizopus species. fus ...200819023814
microbial oxidation of anabolic steroids.microbial transformation of two anabolic steroids, ethylestrenol (1) and nandrolone (2), were carried out. ethylestrenol (1), when incubated with rhizopus stolonifer (tsy 0471), yielded two oxidative metabolites named 17alpha-ethyl-3beta,17beta-dihydroxy-19-norndrost-4-ene (3) and 17alpha-ethyl-17beta-hydroxy-19-norandrost-4-en-3-one (4), while incubation of compound 2 with the same fungus yielded two oxidative metabolites, 19-norandrost-4-en-3,17-dione (5) and 6alpha,17beta-dihydroxy-19-norandr ...200819023784
production and characterization of a monoclonal antibody raised against surface antigens from mycelium of gaeumannomyces graminis var. tritici: evidence for an extracellular polyphenol oxidase.abstract a murine monoclonal antibody (mab) of immunoglobulin class m (igm) was raised against surface antigens from gaeumannomyces graminis var. tritici and, by enzyme-linked immunosorbent assay, recognized isolates of g. graminis var. tritici, g. graminis var. avenae and g. graminis var. graminis. characterization of the antigen by heat and protease treatments showed that the epitope recognized by the mab was a protein. antigen production was detected only in live mycelia. immunofluorescence s ...199718945163
antifungal activity of 2-deoxy-d-glucose on botrytis cinerea, penicillium expansum, and rhizopus stolonifer: ultrastructural and cytochemical aspects.abstract the effect of 2-deoxy-d-glucose on major postharvest pathogens was investigated at the ultrastructural and cytochemical level. hyphae of botrytis cinerea, penicillium expansum,, and rhizopus stolonifer grown in the absence of 2-deoxy-d-glucose were normal and showed no apparent cytological alterations. in the presence of 2-deoxy-d-glucose, however, these fungi exhibited severe cellular injuries ranging from cell wall disruption to cytoplasm disintegration. although 2-deoxy-d-glucose cau ...199718945101
anaerobic formation of fumaric acid by the mold rhizopus nigricans. 194818880574
in vitro antifungal activity and mechanism of action of chitinase against four plant pathogenic fungi.to determine why chitinase has different antifungal activity on different pathogenic fungi in vitro, we purified recombinant rice chitinase from pichia pastoris and investigated its antifungal activity against four fungi - rhizopus stolonifer (ehrenb. et fr.) vuill, botrytis squamosa walker, pythium aphanidermatum (eds.) fitzp, and aspergillus niger van tiegh. scanning electron microscopy (sem) and fourier transform infrared spectroscopy (ftir) were used to analyze the surface microstructure and ...200818720488
evaluation of messenger plant activator as a preharvest and postharvest treatment of sweet cherry fruit under a controlled atmosphere.the preservation methods as an alternative to chemical control to prevent postharvest quality losses of sweet cherry were examined. the efficacy of preharvest and postharvest messenger (m) treatments on sweet cherry cv. '0900 ziraat' was tested under a controlled atmosphere in 2004 and 2005. the factors investigated included the separate or combined effect of low oxygen, high carbon dioxide and m on the quality and fungal pathogens of sweet cherries in a normal atmosphere (na) and in a controlle ...200818671163
headspace sorptive extraction and gc-tofms for the identification of volatile fungal metabolites.volatiles from fungi cultivated in petri dishes were collected by a simple headspace polydimethylsiloxane (pdms) sorptive extraction technique (hsse), thermally desorbed into a gas chromatographic capillary column and detected and identified by gas chromatography-time-of-flight mass spectrometry (gc-tofms). the method was used to compare metabolite profiles of seven species of fungi grown on two types of sterile agars - potato dextrose and sabouraud dextrose. three species from the genus penicil ...200818625272
effect of shear on membrane-associated enzymes: studies of the release of intracellular protein and of the progesterone 11alpha-hydroxylase complex from rhizopus nigricans.rhizopus nigricans atcc 6227b grown in either shake flasks or a fermentor was sheared in a concentric cylinder viscometer. the cells grown in shake flasks were found to be more susceptible to disruption by shear than those grown in the fermentor. cells resuspended in a medium containing reduced glutathione and edta were found to be more easily disrupted than cells resuspended in 0.5% nacl. the optimum condition for disruption of shake flask cells grown in the former medium with retention of prog ...198518553637
effect of shear on membrane-associated enzymes: studies of isolated progesterone 11alpha-hydroxylase complex from rhizopus nigricans. 198518553636
growth of phytopathogenic fungi in the presence of partially acetylated chitooligosaccharides.four phytopathogenic fungi were cultivated up to six days in media containing chitooligosaccharide mixtures differing in average dp and fa. the three different mixtures were named q3 (which contained oligosaccharides of dp2-dp10, with dp2-dp7 as main components), q2 (which contained oligosaccharides of dp2-dp12, with dp2-dp10 as main components) and q1 (which derived from q2 and contained oligomers of dp5-dp8 with hexamer and a heptamer as the main components). the novel aspect of this work is t ...200818523865
is allergenic similarity predictable in respiratory allergies?first degree relatives of patients with allergic diseases are at increased risk of having the disorder. however, it is not clear whether two such related patients with allergic diseases are sensitive to the same antigens or not.200818479656
[five cases of zygomycosis.].combined histological and mycological study of tissue specimens established a proven diagnosis of cutaneous zygomycosis in four patients. all patients had been treated with wide spectrum antibiotics and one patient (liver transplantation) was in addition also treated with cyclosporine. all had acidosis and cutaneous breaks and four had also been treated with systemic corticosteroids. the infecting organisms were absidia corymbifera (n=2), rhizopus stolonifer (n=1) and mucor circinelloides (n=1). ...199918473594
fungal transformation of dydrogesterone and inhibitory effect of its metabolites on the respiratory burst in human neutrophils.the biotransformation of the synthetic hormone dydrogesterone (1) by a number of fungal strains - including cephalosporium aphidicola, rhizopus stolonifer, cunninghamella elegans, and fusarium lini - afforded ten different metabolites, compounds 2-11. from a structural point of view, these transformations involved a combination of hydroxylation, oxidation, reduction, and/or epoxidation. the pregnane-based metabolites 10 and 11 are new compounds. all the known compounds were obtained for the firs ...200818293446
high-affinity iron permease (ftr1) gene sequence-based molecular identification of clinically important zygomycetes.the clinical importance of zygomycosis, an emerging and frequently fatal mycotic disease, has increased during recent years. this report describes an identification method based on pcr amplification and sequencing of the high-affinity iron permease 1 gene (ftr1). primers and amplification protocols were established and tested for the identification of rhizopus oryzae, rhizopus microsporus var. rhizopodiformis, r. microsporus var. oligosporus, rhizopus schipperae, rhizopus niveus and rhizopus sto ...200818190575
quantifying fungal viability in air and water samples using quantitative pcr after treatment with propidium monoazide (pma).a method is described to discriminate between live and dead cells of the infectious fungi aspergillus fumigatus, aspergillus flavus, aspergillus terreus, mucor racemosus, rhizopus stolonifer and paecilomyces variotii. to test the method, conidial suspensions were heat inactivated at 85 degrees c or held at 5 degrees c (controls) for 1 h. polycarbonate filters (25 mm diameter, 0.8 microm pore size) were placed on "welled" slides (14 mm diameter) and the filters treated with either pbs or pma. pro ...200818160156
carbon dioxide inhibition of anaerobic fumarate formation in the mold rhizopus nigricans. 194918113497
bioremediation of aflatoxins by some reference fungal strains.aspergillus parasiticus rcmb 002001 (2) producing four types of aflatoxins b1, b2, g1, and g2 was used in this study as an aflatoxin-producer. penicillium griseofulvum, p. urticae, paecilomyces lilacinus, trichoderma viride, candida utilis, saccharomyces cerevisiae as well as a non-toxigenic strain of aspergillus flavus were found to be able to exhibit growth on aflatoxin b1-containing medium up to a concentration of 500 ppb. it was also found that several fungal strains exhibited the growth in ...200718062656
microbial hydroxylation of sclareol by rhizopus stolonifer.incubation of sclareol with rhizopus stolonifer affords in high yield a mixture of triols with 18-hydroxy-sclareol as the main component.200518007369
in vitro antioxidant and anti-rhizopus activities of lamiaceae herbal extracts.eighty-eight extracts of different polarity obtained from 18 lamiaceae medicinal and aromatic plants were screened for their antioxidant and antifungal properties. a tlc analysis combined with two bioassays were used to detect these activities. in most cases the highest radical scavenger capacity was detected in methanolic and aqueous extracts and polyphenols may be responsible. the most interesting antioxidant activity was observed in polar extracts obtained from lycopus europaeus, melissa offi ...200717912643
biosorption of simulated dyed effluents by inactivated fungal biomasses.treatment of dyed effluents presents several problems mainly due to the toxicity and recalcitrance of dyestuffs. innovative technologies, such as biosorption, are needed as alternatives to conventional methods to find inexpensive ways of removing dyes from large volumes of effluents. inactivated biomasses do not require a continuous supply of nutrients and are not sensitive to the toxicity of dyes or toxic wastes. they can also be regenerated and reused in many cycles and are both safe and envir ...200817888654
biotransformations of imbricatolic acid by aspergillus niger and rhizopus nigricans cultures.microbial transformation of imbricatolic acid (1) by aspergillus niger afforded 1alpha-hydroxyimbricatolic acid (2), while transformation with rhizopus nigricans yielded 15-hydroxy-8,17-epoxylabdan-19-oic acid (3). when the diterpene 1 was added to a cunninghamella echinulata culture, the main products were the microbial metabolites mycophenolic acid (4) and its 3-hydroxy derivative 5. all the structures were elucidated by spectroscopic methods. the cytotoxicity of these compounds towards human ...200717873843
antifungal lactic acid bacteria with potential to prolong shelf-life of fresh vegetables.the aim of this study was to isolate and identify antifungal lactic acid bacteria from fresh vegetables, and evaluate their potential in preventing fungal spoilage of vegetables.200717850302
longevity in spores of aspergillus oryzae and rhizopus nigricans. 192317774267
zygospores and sexual strains in the common bread mould, rhizopus nigricans. 190617772189
fumaric acid formation associated with sexuality in a strain of rhizopus nigricans. 193917732505
mycoflora of two types of portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate.the mycoflora of chouriqo types alentejano and ribatejano, two varieties of portuguese dry-smoked sausages, have been investigated after a producer-defined shelf life period (120 days at 20 +/- 5 degrees c) in modified atmosphere packaging (55% n2 and 45% co2). on the basis of morphological and physiological characteristics, the isolates were identified as penicillium, aspergillus, fusarium, rhizopus, monilia, absidia, and cephalosporium. the species identified were as follows: penicillium terre ...200717612078
steroid extraction in a microchannel system--mathematical modelling and experiments.the continuous ethyl acetate extraction of progesterone and 11alpha-hydroxyprogesterone, a reactant and the product of the biotransformation step involved in corticosteroid production, was studied in a microchannel at different flow velocities. in addition, non-steady state batch extraction without mixing was performed and modelled in order to verify the theoretically predicted parameters. in order to analyze experimental data and to forecast microreactor performance, a three-dimensional mathema ...200717594008
the identification of antibacterial compounds for the development of enhanced media for the detection of foodborne fungi.in an effort to identify a more suitable antibiotic for utilization in mycological media, 12 food borne fungal species from various genera including alternaria alternata, aspergillus flavus, a. niger, eurotium chevalieri, fusarium moniliforme, penicillium sp., rhizopus stolonifer, saccharomyces cerevisiae, candida tropicalis, geotrichum candidum, rhodotorula glutinis and kluyveromyces thermotolerans along with 21 chloramphenicol-resistant bacterial isolates from fresh produce and atcc cultures o ...200717574697
suppression of phytopathogenic fungi by hexane extract of nepenthes ventricosa x maxima leaf.the hexane extract of nepenthes ventricosa x maxima leaf exhibited antifungal activity against alternaria alternata, aspergillus niger, bipolaris oryzae, fusarium oxysporum, phytophthora capsici, rhizoctonia solani, rhizopus stolonifer var. stolonifer and sclerotinia sclerotiorum.200717573203
[cloning and expression of a delta6-fatty acid desaturase gene from rhizopus stolonifer in saccharomyces cervisiae].fatty acid composition of fungi is analysed through the gas chromatography( gc) technique. with specific activity a novel enzyme delta6-fatty acid desaturase was screened and isolated from rhizopus stolonifer. in this study r. stolonifer was identified as a fungal species that produced plentiful gamma-linolenic acid. a 1475bp full-length cdna, designated as rnd6d here, with high homology to fungal delta6-fatty acid desaturase genes was isolated from r. stolonifer using reverse transcription poly ...200717436625
microbial transformation of the steroidal alkaloid dictyophlebine by rhizopus stolonifer.the microbial transformation of a steroidal alkaloid, dictyophlebine (1) with rhizopus stolonifer (atcc 10404) afforded three oxidized metabolites 2-4. compound 2 was found to be a new product. these metabolites were structurally characterized on the basis of modern spectroscopic techniques. their inhibitory activity towards acetyl- and butyrylcholinesterase has been evaluated and the new product 2 has been found to be more potent than the parent compound and other metabolites.200717409573
composition and antifungal activity of essential oils of mentha piperita and lavendula angustifolia on post-harvest phytopathogens.the general antifungal activity of essential oils is well documented. the advantage of essential oils is their bioactivity in the vapor phase, a characteristic that makes them attractive as possible fumigants for stored product protection. essential oils of aerial parts of mentha piperita and lavendula angustifolia were obtained with hydrodistillation and oils composition identified with gc-ms. menthanol (36.24%) and menthone (32.42%) were the major compounds of the m. piperata essential oil. th ...200617390896
microbial transformation of (-)-guaiol and antibacterial activity of its transformed products.microbial transformation of the sesquiterpene (-)-guaiol (1) [1(5)-guaien-11-ol] was investigated using three fungi, rhizopus stolonifer, cunninghamella elegans, and macrophomina phaseolina. fungal transformation of 1 with rhizopus stolonifer yielded a hydroxylated product, 1-guaiene-9 beta,11-diol (2). in turn, cunninghamella elegans afforded two mono- and dihydroxylated products, 1-guaiene-3beta,11-diol (3) and 1(5)-guaiene-3beta,9 alpha,11-triol (4), while macrophomina phaseolina produced two ...200717385913
polyphasic approach to the taxonomy of the rhizopus stolonifer group.phylogenetic analysis of rhizopus strains based on the d1/d2 region of lsu rdna sequences yielded a phylogram with four well-supported clades. the r. microsporus clade concurs with classification obtained by traditional methods. the r. oryzae group was found to include species of the genus amylomyces. the traditional r. stolonifer group was divided into two well-supported clades in the phylogram, with one clade comprising r. stolonifer var. stolonifer, r. sexualis var. sexualis, and r. sexualis ...200717324757
association between sensitization to aureobasidium pullulans (pullularia sp) and severity of asthma.recent data indicate that fungi may contribute to increased severity of asthma.200717304882
interactions between statins and penicillium chrysogenum antifungal protein (paf) to inhibit the germination of sporangiospores of different sensitive zygomycetes.this study reports on the antifungal activities of statins combined with an antifungal compound secreted by penicillium chrysogenum, paf. several species belonging in the class zygomycetes are considered to be agents of human or animal mycoses; other species have significance as postharvest plant pathogens. in the present work, four species (rhizopus stolonifer, mortierella wolfii, syncephalastrum racemosum and mycotypha africana) that exhibited different sensitivities to lovastatin and paf in p ...200717302920
synthesis of pseudopeptides based l-tryptophan as a potential antimicrobial agent.four compounds named l-btrppa, l-trp-o-pa, l-trp-m-pa and l-trp-p-pa, pseudopeptides constructed from pyridine and tryptophan units, were synthesized and tested against the gram-positive, gram-negative strains of bacteria and human pathogenic fungi. l-trp-o-pa proved to be a broad-spectrum antimicrobial agent, showing a significant inhibition of the growth of gram-positive bacteria (staphylococcus aureus, methicillin-resistant s. aureus, bacillus subtilis, micrococcus luteus), and pathogenic fun ...200717257839
the microbial hydroxylation of levonorgestrel.the microbial transformation of levonorgestrel (1) by cunningham elegans resulted in the formation of five hydroxylated metabolites, 13-ethyl-10beta, 17beta-dihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one(2), 13-ethyl-6beta,17beta-dihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one (3) 13-ethyl 6beta, 10beta, 17beta-trihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one (4) 13-ethyl-15alpha-17beta-dihydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one (5) and 13-ethyl-11alpha, 17beta-dihydrox ...200617201044
structure elucidation and antibacterial activity of new fungal metabolites of sclareol.the transformation of the antibacterial diterpene sclareol (1) by two different fungal strains was investigated (scheme). in the presence of rhizopus stolonifer, (3beta)-3-hydroxysclareol (2), 18-hydroxysclareol (3), (6alpha)-6,18-dihydroxysclareol (4), and (11s)-11,18-dihydroxysclareol (5) were formed. fermentation of 1 with fusarium lini afforded (1beta)-1-hydroxysclareol (6) and (12s)-12-hydroxysclareol (7). compounds 4-7 were identified as new compounds, and some of them were active against ...200617193216
fungal metabolites of (e)-guggulsterone and their antibacterial and radical-scavenging activities.fungal transformation of (e)-guggulsterone (= (17e)-pregna-4,17-diene-3,16-dione; 1) by rhizopus stolonifer, fusarium lini, cunninghamella elegans, or gibberella fujikuroi afforded ten hydroxylated metabolites (2-11; scheme), which were fully characterized. compounds 4-11 have not been described yet. some of these novel hydroxylated metabolites, as well as acetylated derivatives thereof, exhibited significant antibacterial and radical-scavenging activities (table 3).200517192001
effect of yeast antagonist in combination with heat treatment on postharvest blue mold decay and rhizopus decay of peaches.the potential of using heat treatment alone or in combination with an antagonistic yeast for the control of blue mold decay and rhizopus decay of peaches caused by penicillium expansum and rhizopus stolonifer respectively, and in reducing natural decay development of peach fruits, as well as its effects on postharvest quality of fruit was investigated. in vitro tests, spore germination of pathogens in pdb was greatly controlled by the heat treatment of 37 degrees c for 2 d. in vivo test to contr ...200717140691
characterization of some mushroom and earthy off-odors microbially induced by the development of rot on grapes.grape rot is one of the major causes of degradation of many grape components and, thus, of deterioration in wine quality. in particular, the association of botrytis cinerea with other, less visible, fungi frequently leads to the development of organoleptic defects in grapes and sometimes in wines. this study examines the nature of the volatile compounds responsible for mushroom, mossy, or earthy odors detected by gas chromatography-olfactometry in organic extracts of rotten grapes and musts. 2-m ...200617117809
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