Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| transmembrane ca2+ fluxes associated with zoospore encystment and cyst germination by the phytopathogen phytophthora parasitica | phytophthora spp. can infect plants within 30 min of a zoospore arriving at a host surface. the infection sequence from zoospore encystment to cyst germination and host penetration seems to be largely preprogrammed and dependent on calcium signaling. here we present evidence that the encystment process leading to autonomous germination in phytophthora parasitica may be coordinated by sequential net ca2+ fluxes across the cell membrane, which we assessed by fluorimetry of the probe fura-2 in zoos ... | 1998 | 9806806 |
| occurrence of a parasexual cycle following the transfer of isolated nuclei into protoplasts of phytophthora parasitica. | nuclei isolated from metalaxyl-resistant (mr) protoplasts of phytophthora parasitica were transferred into chloroneb-resistant (cnr) protoplasts of the same species, with an average successful rate of 1x10(-4), using a selective medium containing both metalaxyl and chloroneb. no colonies appeared when self-fusion products of donor nuclei or recipient protoplasts were exposed to the selective medium. similar results were obtained when the reciprocal nuclear transformation was performed. all the z ... | 1998 | 9724414 |
| use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of phytophthora parasitica var nicotianae | the relationship between the production of reactive oxygen species and the hypersensitive response (hr) of tobacco (nicotiana tabacum l. ) toward an incompatible race of the oomycete phytophthora parasitica var nicotianae has been investigated. a new assay for superoxide radical (o2-) production based on reduction of the tetrazolium dye sodium,3'-(1-[phenylamino-carbonyl]-3, 4-tetrazolium)-bis(4-methoxy-6-nitro) benzene-sulfonic acid hydrate (xtt) has enabled the quantitative estimation of perhy ... | 1998 | 9625702 |
| the incompatible interaction between phytophthora parasitica var. nicotianae race 0 and tobacco is suppressed in transgenic plants expressing antisense lipoxygenase sequences. | nicotiana tabacum 46-8 cultivar displays an incompatible interaction with race 0 of phytophthora parasitica var. nicotianae (ppn), a fungal pathogen of most tobacco cultivars. at the plant level, incompatibility is characterized by the induction of lipoxygenase (lox, ec = 1.13.11.12) activity and localized hypersensitive cell death before defense gene activation. to evaluate the involvement of lox in the onset of plant defense, tobacco 46-8 plants were genetically engineered using full-length or ... | 1998 | 9601005 |
| rnase activity prevents the growth of a fungal pathogen in tobacco leaves and increases upon induction of systemic acquired resistance with elicitin. | the hypersensitive response and systemic acquired resistance (sar) can be induced in tobacco (nicotiana tabacum l.) plants by cryptogein, an elicitin secreted by phytophthora cryptogea. stem application of cryptogein leads to the establishment of acquired resistance to subsequent leaf infection with phytophthora parasitica var nicotianae, the agent of the tobacco black shank disease. we have studied early events that occur after the infection and show here that a tobacco gene encoding the extrac ... | 1997 | 9414563 |
| cloning and characterization of a cdna encoding an elicitor of phytophthora parasitica var. nicotianae that shows cellulose-binding and lectin-like activities. | phytophthora parasitica var. nicotianae produces a 34-kda glycoprotein elicitor (cbel) that is localized in the cell wall. a cdna encoding the protein moiety of this elicitor was cloned and characterized. the deduced amino acid sequence consisted of two direct repeats of a cysteine-rich domain, joined by a thr/pro-rich region. although having no general homology with published sequences, the positions of the cysteine residues in the two repeats show a conserved pattern, similar to that of the ce ... | 1997 | 9390419 |
| mating-type loci segregate aberrantly in phytophthora infestans but normally in phytophthora parasitica: implications for models of mating-type determination. | in the oomycete, phytophthora infestans, mating type is determined by a locus that segregates in a non-mendelian manner consistent with its linkage to a system of balanced lethals. the significance of this unusual phenomenon was addressed by studying the segregation patterns of dna markers linked to mating type in the related species, p. parasitica. this was done using loci identified by either rapd analysis of p. parasitica crosses or by cross-hybridization with rflp markers linked to mating ty ... | 1997 | 9309171 |
| isolation and characterization of inositol sphingophospholipids from phytophthora parasitica dastur. | several inositol sphingophospholipids (ispl) were isolated from mycelia of phytophthora parasitica dastur, a phytopathogenic fungus of carnation. the ispl structures were determined by fast atom bombardment. all ispl consisted of ceramides linked to inositol phosphate. we investigated the effect of growth conditions on the ispl produced in four different media that are commonly used for fungal cultures. we showed that p. parasitica dastur synthesized four major classes of compounds with molecula ... | 1997 | 9113622 |
| a peroxidase gene promoter induced by phytopathogens and methyl jasmonate in transgenic plants. | the expression of two closely related peroxidase isogenes, shpx6a and shpx6b, of the legume stylosanthes humilis was studied using isogene-specific reverse transcriptase pcr techniques. results indicated that transcripts of both genes were rapidly induced following inoculation with the fungal pathogen colletotrichum gloeosporioides, wounding and treatment with the defense regulator methyl jasmonate (meja). in contrast treatment of leaves of s. humilis with abscisic acid (aba) and salicylic acid ... | 1997 | 9100378 |
| lipoxygenase gene expression in the tobacco-phytophthora parasitica nicotianae interaction. | a recently isolated cdna clone of tobacco (nicotiana tabacum l.) lipoxygenase (lox) was used to study lox gene expression in tobacco cell-suspension cultures and intact plants in response to infection with phytophthora parasitica nicotianae (ppn). southern blot analysis of tobacco dna indicated that only a small number of lox genes hybridize to this probe. these genes were not constitutively expressed to a detectable level in control cells and healthy plants. in contrast, a rapid and transient a ... | 1996 | 8938408 |
| characterization of a gene cluster of phytophthora cryptogea which codes for elicitins, proteins inducing a hypersensitive-like response in tobacco. | elicitins, proteinaceous elicitors secreted by phytophthora spp., act as inducers of a hypersensitive-like response in tobacco during incompatible interactions. we have isolated and cloned sequences encoding cryptogein and related isoforms from p. cryptogea that belong to the elicitin family. the isolation of a genomic clone led to the characterization of four clustered genes. two of these genes encode distinct elicitins, and two genes would encode, if expressed, a class of highly acidic eliciti ... | 1995 | 8664508 |
| increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a. | expression of pathogenesis-related protein 1a (pr-1a), a protein of unknown biochemical function, is induced to high levels in tobacco in response to pathogen infection. the induction of pr-1a expression is tightly correlated with the onset of systemic acquired resistance (sar), a defense response effective against a variety of fungal, viral, and bacterial pathogens. while pr-1a has been postulated to be involved in sar, and is the most highly expressed of the pr proteins, evidence for its role ... | 1993 | 8346252 |
| a gene encoding a host-specific elicitor protein of phytophthora parasitica. | extracellular elicitor proteins (elicitins) from phytophthora species induce local and distal defense responses specifically in plants of the solanaceae and cruciferae. based on elicitin amino acid sequences, elicitin-coding sequences from p. parasitica were amplified by the polymerase chain reaction. a genomic clone containing a complete elicitin gene, para1, was isolated and sequenced. elicitin was confirmed to be encoded as a precursor protein containing a 20-amino acid signal peptide that is ... | 1993 | 8274771 |
| pcr amplification of species-specific dna sequences can distinguish among phytophthora species. | we used pcr to differentiate species in the genus phytophthora, which contains a group of devastating plant pathogenic fungi. we focused on phytophthora parasitica, a species that can infect solanaceous plants such as tomato, and on phytophthora citrophthora, which is primarily a citrus pathogen. oligonucleotide primers were derived from sequences of a 1,300-bp p. parasitica-specific dna segment and of an 800-bp p. citrophthora-specific segment. under optimal conditions, the primers developed fo ... | 1994 | 8074533 |
| structural features of fungal beta-d-glucans for the efficient inhibition of the initiation of virus infection on nicotiana tabacum. | glucans of fungal origin have been shown to inhibit the early stages of infection of nicotiana by numerous viruses of different taxonomic groups. several glucans were isolated from the cell walls of phytophthora parasitica, phytophthora megasperma f. sp. glycinea (pmg) and fusarium oxysporum, and their antiviral activity compared on tobacco leaves inoculated with tobacco mosaic virus. these polysaccharides consist of a mixture of (1-->3)(1-->6)-beta-d-glucans with m(r) varying from 1.1 x 10(3) t ... | 1995 | 7786491 |
| elicitin produced by an isolate of phytophthora parasitica pathogenic to tobacco. | elicitin was prepared from a phytophthora parasitica culture isolated from tobacco in australia. the protein with elicitor activity was purified and sequenced. this protein, which contains 98 amino acid residues, shows full homology with an elicitin produced by a completely unrelated isolate from carnation, indicating conservation of elicitin sequence within a single species. | 1995 | 7766061 |
| new antibiotics phthoxazolins b, c and d produced by streptomyces sp. ko-7888. | new antibiotics, phthoxazolins b, c and d were isolated from the fermentation broth of streptomyces sp. ko-7888. they are geometrical isomers of 10-hydroxyphthoxazolin a. they showed selective antifungal activity against phytophthora parasitica in vitro and modest herbicidal activity in a laboratory test, but the potencies were different among isomers. | 1995 | 7649873 |
| [isolation and structural studies of glucans from phytophthora parasitica]. | several polysaccharides have been isolated from the cell walls of phytophthora parasitica, a phytopathogenic fungus of carnation. the crude polysaccharides were fractionated by successive chromatographies on deae-cellulose, sephadex g-25, concanavalin-a-sepharose and sephadex g-200 columns. the neutral polysaccharides consist of a mixture of beta(1----3, 1----6)-d-glucans whose relative molecular masses varied from 9000 to about 200 000. all these polysaccharides have a main chain of beta(1----3 ... | 1984 | 6745270 |
| serological differentiation of pythium aphanidermatum from phytophthora parasitica var. nicotiane and ph parasitica. | 1967 | 6030060 | |
| fine structure of phytophthora parasitica zoospores. | 1969 | 5772251 | |
| ultrastructural aspects of encystation and cyst-germination in phytophthora parasitica. | 1971 | 5565061 | |
| sterol or calcium requirement by phytophthora parasitica var. nicotianae for growth on nitrate nitrogen. | 1970 | 5441001 | |
| cation and sterol effects on growth of phytophthora parasitica var. nicotianae. | 1969 | 4985015 | |
| efficacy of certain antibiotics against phytophthora parasitica var. piperina causing leaf-rot of pan. | 1973 | 4786731 | |
| production of oospores by phytophthora parasitica in liquid medium. | 1974 | 4444724 | |
| ultrastructural changes during zoospore formation in phytophthora parasitica. | 1967 | 4168773 | |
| antitumor active beta-d-glucans from phytophthora parasitica. | 1988 | 3378236 | |
| antimicrobial activity of naphthoquinones from fusaria. | twenty-two naphthoquinone compounds isolated or derived synthetically from culture extracts of fusarium solani and f. oxysporum were examined for antimicrobial activity. fifteen exhibited antibiotic activity against staphylococcus aureus, and 12 were active against streptococcus pyogenes, but none were active at the highest rate of 128 micrograms/ml against escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, serratia marcescens, or pseudomonas aeruginosa. of 8 plant path ... | 1990 | 2233983 |
| tandem arrangement of trna(asp)-encoding genes in phytophthora spp. | we have cloned a region of repetitive dna from the phytopathogenic fungus, phytophthora parasitica. the cloned region consists of 17 highly homologous units arranged in tandem. the consensus sequence is 562 bp long and carries the information for a trna(asp). all sequence motifs required for efficient rna polymerase iii transcription are present, and the trna derived from the nucleotide sequence is able to form a complete cloverleaf structure with high homology to previously characterized trna(a ... | 1991 | 1864509 |
| the trp1 gene of phytophthora parasitica encoding indole-3-glycerolphosphate synthase-n-(5'-phosphoribosyl)anthranilate isomerase: structure and evolutionary distance from homologous fungal genes. | the trp1 gene was isolated from the genome of phytophthora parasitica. it encodes bifunctional enzyme of the tryptophan biosynthetic pathway indole-3-glycerolphosphate synthase-n-(5'-phosphoribosyl)anthranilate isomerase (igps-prai). the gene was localized and sequenced using random in vitro insertions of omega interposon. the domain structure of the protein product was found to be similar to that of enteric bacteria but different from the structure of homologous enzymes in fungi. two introns in ... | 1991 | 1756978 |
| genetic transformation of the plant pathogens phytophthora capsici and phytophthora parasitica. | phytophthora capsici and p.parasitica were transformed to hygromycin b resistance using plasmids pcm54 and phl1, which contain the bacterial hygromycin b phosphotransferase gene (hph) fused to promoter elements of the ustilago maydis heat shock hsp70 gene. enzymes driselase and novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid dna and polyethylene glycol 6000. transformation frequencies of over 500 transformants per micrograms of dna per 1 x ... | 1991 | 1651483 |
| genetic code and phylogenetic origin of oomycetous mitochondria. | we sequenced the 3'-terminal part of the cox3 gene encoding cytochrome c oxidase subunit 3 from mitochondria of phytophthora parasitica (phylum oomycota, kingdom protoctista). comparison of the sequence with known cox3 genes revealed that ugg is used as a tryptophan codon in contrast to uga in the mitochondrial codes of most organisms other than green plants. a very high at mutation pressure operates on the mitochondrial genome of phytophthora, as revealed by codon usage and by a+t content of no ... | 1992 | 1588598 |
| activity of some antibiotics against phytophthora parasitica var. piperina, incitant of foot-rot and leaf-rot of pan. | 1975 | 1236088 | |
| organic amendments and control of foot rot of piper betle caused by phytophthora parasitica var. piperina. | experiments have been performed to control foot rot of piper betle by organic amendments and antagonists in soil. soil amended with corn straw gave best control. corn straw supplemented with nitrogen (nh4no3) gave still better control. the effect of different antagonists growing on corn-straw and til (sesamum indicum) oil cake amendments showed that trichoderma viride gave best control. aspergillus terreus failed to control the disease although it showed great antifungal activity under controlle ... | 1976 | 952442 |
| lack of kestose (fructosylcucrose) production by race 3 of phytophthora parasitica var. nicotianae differentiates it from races 0 and 1. | 1977 | 895761 | |
| an examination of enzyme production of phytophthora spp. on solid and liquid media. | solid media were used to determine which extracellular hydrolytic enzymes are produced by phytophthora parasitica; p. parasitica var. nicotianae, races 0, 1, and 3; and several other phytophthora spp. most isolates produced rnase, dnase, phosphatase, lipase, and cellulase uniformly. all race 3 isolates of p. parasitica var. nicotianae exhibited protease activity on a medium containing gelatin, while only 33 and 60% of the race 0 and 1 isolates, respectively, and 14% of the p. parasitica isolates ... | 1978 | 754880 |
| polar lipids of phytophthora parasitica var. nicontianae, in comparison with those of other selected fungi. | 1976 | 133289 |