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effect of methyl butyrate aroma on the survival and viability of human breast cancer cells in vitro.aroma can have far reaching effects on mind, body and soul. pleasant aromas are known to have a soothing effect on the mind and are known to relieve stress and enhance concentration. recently, it has been demonstrated that aroma may also have some curative effects as well as benefits and can be used both for prophylaxis and therapy of diseases. our aim was to test our hypothesis whether aroma can cure or prevent cancer. methyl butyrate (mb) is the methyl ester of butyric acid having a characteri ...201627106628
comparative study of the physicochemical, compositional and functional properties of eight flours obtained from different plant materials found in colombia.the physicochemical, compositional and functional properties of flour from green plantains (musa acuminata) of the large green plantain variety, oyster mushrooms (pleorotus ostreatus), pineapple peel (ananas comosus) of the 'apple pineapple' variety, yellow peas (pisum sativum), chickpeas (cicer arietinum), whole grain rice (oryza sativa), whole grain corn (zea mays) and whole grain white quinoa (chenopodium quinoa) were evaluated by using one-way analysis of variance, pearson correlations and p ...201627091150
current status of tropical fruit breeding and genetics for three tropical fruit species cultivated in japan: pineapple, mango, and papaya.tropical fruit crops are predominantly produced in tropical and subtropical developing countries, but some are now grown in southern japan. pineapple (ananas comosus), mango (mangifera indica) and papaya (carica papaya) are major tropical fruits cultivated in japan. modern, well-organized breeding systems have not yet been developed for most tropical fruit species. most parts of japan are in the temperate climate zone, but some southern areas such as the ryukyu islands, which stretch from kyushu ...201627069392
dietary supplementation of green synthesized manganese-oxide nanoparticles and its effect on growth performance, muscle composition and digestive enzyme activities of the giant freshwater prawn macrobrachium rosenbergii.the green synthesized mn3o4 nanoparticles (manganese-oxide nanoparticles) using ananas comosus (l.) peel extract was characterized by various techniques. hr-sem photograph showed that manganese-oxide nanoparticles (mn-oxide nps) were spherical in shape, with an average size of 40-50 nm. the zeta potential revealed the surface charge of mn-oxide nps to be negative. further, the mn-oxide nps were dietary supplemented for freshwater prawn macrobrachium rosenbergii. the experimental basal diets were ...201627049122
the physico-chemical properties of some citrus seeds and seed oils.the chemical properties, mineral contents, fatty acid and tocopherol contents of seed and seed oils of some citrus genus provided from several locations in turkey and saudi arabia were determined. while ca contents of seeds were between 5018 mg/kg (kütdiken lemon) and 7619 mg/kg (kinnow mandarin), k contents of seeds varied between 7007 mg/kg (orlando orange) and 10334 mg/kg (kinnow mandarin). glucose and fructose contents of citrus seed samples varied between 3.75 g/kg and 5.75 g/kg, and 4.09 g ...201627023319
in vivo evaluation of skin irritation potential, melasma and sebum content following long term application of skin care cream in healthy adults, using non-invasive biometrological techniques.the present investigation was conducted to evaluate non-invasively, various functional skin parameters i.e., irritation potential, melasma and sebum contents following long term application of topical cream (w/o) loaded with 2% methanolic extract of ananas comosus l. versus placebo control (base) in healthy adults. healthy human volunteers (n = 11, aged 20-30 years) were recruited for investigation and written informed consent was taken from each volunteer. in this single blinded study every vol ...201627008816
antibacterial and antiproliferative peptides in synbiotic yogurt-release and stability during refrigerated storage.the search for alternative therapeutics is on the rise due to the extensive increase in bacterial resistance to various conventional antibiotics and side effects of conventional cancer therapies. bioactive peptides released from natural sources such as dairy foods by lactic acid bacteria have received attention as a potential source of biotherapeutic peptides. however, liberation of peptides in yogurt depends on proteolytic activities of the cultures used. thus, this research was conducted to es ...201626995128
physicochemical, functional and antioxidant properties of tropical fruits co-products.the aim of this study was to determine the physicochemical, functional and antioxidant properties of mango (mac), pineapple (pac) and passion fruit (pfc) co-products in order to evaluate them as ingredients for food application. proximate composition showed low fat content (0.95-5.64 g/100 g), and high levels of dietary fiber. in pineapple and passion fruit co-products, dietary fiber represented more than 50 % of the sample. low ph, water activity, along with high acidity indicated that these co ...201626984339
de novo transcriptome assembly of floral buds of pineapple and identification of differentially expressed genes in response to ethephon induction.a remarkable characteristic of pineapple is its ability to undergo floral induction in response to external ethylene stimulation. however, little information is available regarding the molecular mechanism underlying this process. in this study, the differentially expressed genes (degs) in plants exposed to 1.80 ml·l(-1) (t1) or 2.40 ml·l(-1) ethephon (t2) compared with ct plants (control, cleaning water) were identified using rna-seq and gene expression profiling. illumina sequencing generated 6 ...201626955375
comparative study of antitumor effects of bromelain and papain in human cholangiocarcinoma cell lines.cholangiocarcinoma (cc) worldwide is the most common biliary malignancy with poor prognostic value and new systemic treatments are desirable. plant extracts like bromelain and papain, which are cysteine proteases from the fruit pineapple and papaya, are known to have antitumor activities. therefore, in this study for the first time we investigated the anticancer effect of bromelain and papain in intra- and extrahepatic human cc cell lines. the effect of bromelain and papain on human cc cell grow ...201626935541
odors identification differences in deficit and nondeficit schizophrenia.there is evidence that deficit schizophrenia (ds) is associated with neuroanatomical changes in structures including those involved in olfaction. olfactory dysfunction, which includes impaired odor identification, is found in patients with schizophrenia and their family members.201626922544
[detection of syrup adulterants in prepackaged pure pineapple juice by fourier-transform infrared spectroscopy and chemometric analysis].this study was performed to establish a method that can quickly and accurately identify adulterated syrup in the pure pineapple juice. a attenuated total internal refraction-fourier transform infrared spectroscopy was used to collect the range of 900 -1 500 cm(-1) infrared spectra of 234 samples pure pineapple juice and adulterated syrup by beet syrup, rice syrup and cassava syrup. by using linear discriminant analysis and support vector machine for the identification model, comparing the full s ...201526904809
phenylpropanoid glycosides from the leaves of ananas comosus.two new phenylpropanoid glycosides, named β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl β-d-6'-o-acetylglucopyranoside (1) and β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl α-d-glucopyranoside (2), along with two known analogues (3-4) and four glycerides (5-8), were isolated from the etoac extract of the leaves of ananas comosus. their structures were elucidated on the basis of 1d- and 2d-nmr analyses, as well as hr-esi-ms experiments. compounds 1-4 showed significant antibacterial activ ...201526882684
growth potential of listeria monocytogenes and staphylococcus aureus on fresh-cut tropical fruits.the objective of this study was to evaluate the fate of staphylococcus aureus, listeria monocytogenes, and natural microbiota on fresh-cut tropical fruits (pitaya, mango, papaya and pineapple) with commercial pvc film at different storage temperature (5, 13, and 25 °c). the results showed that s. aureus, l. monocytogenes, and natural microbiota increased significantly on fresh-cut tropical fruits at 25 °c. both pathogen and natural microbiota were able to grow on fresh-cut tropical fruits at 13 ...201526862605
an electrochemical impedance spectroscopy system for monitoring pineapple waste saccharification.electrochemical impedance spectroscopy (eis) has been used for monitoring the enzymatic pineapple waste hydrolysis process. the system employed consists of a device called advanced voltammetry, impedance spectroscopy & potentiometry analyzer (avispa) equipped with a specific software application and a stainless steel double needle electrode. eis measurements were conducted at different saccharification time intervals: 0, 0.75, 1.5, 6, 12 and 24 h. partial least squares (pls) were used to mod ...201626861317
evaluation of the enzymatic activity and stability of commercial bromelain incorporated in topical formulations.bromelain is a mixture of proteolytic enzymes found in various tissues of the pineapple plant (ananas comosus) and other species of bromeliaceae. owing to its proteolytic activity, bromelain has been used in the food, medical, pharmaceutical and cosmetic industries, for its cell renewal, anti-ageing, whitening and anti-cellulite properties.201626833020
inactivation of escherichia coli, listeria monocytogenes, and salmonella enteritidis by cymbopogon citratus d.c. stapf. essential oil in pineapple juice.in the present study, the efficacy of cymbopogon citratus d.c. stapf. essential oil (cceo) to provoke a 5-log cfu/ml (5-log) inactivation in a mixed composite of escherichia coli, listeria monocytogenes, and salmonella enterica serovar enteritidis in pineapple (ananas comosus (l.) merril) juice (4°c) was assessed. moreover, the effects of cceo on the physicochemical and sensory quality parameters of pineapple juice were evaluated. the mic of cceo was 5 μl/ml against the composite mix examined. f ...201626818981
phyllotaxis: a framework for foam topological evolution.phyllotaxis describes the arrangement of florets, scales or leaves in composite flowers or plants (daisy, aster, sunflower, pinecone, pineapple). as a structure, it is a geometrical foam, the most homogeneous and densest covering of a large disk by voronoi cells (the florets), constructed by a simple algorithm: points placed regularly on a generative spiral constitute a spiral lattice, and phyllotaxis is the tiling by the voronoi cells of the spiral lattice. locally, neighboring cells are organi ...201626810397
relevance of the mention of antioxidant properties in yogurt labels: in vitro evaluation and chromatographic analysis.the purpose of the inclusion of fruit (natural additives) in yogurt aims to increase its antioxidant activity and functionality. herein, a comparative study of the antioxidant potential of yogurts with pieces of various fruits was performed, including yogurts with mention of antioxidant properties in the label. free radicals scavenging activity, reducing power and inhibition of lipid peroxidation were evaluated by in vitro assays, as were the contents in antioxidants such as phenolics, flavonoid ...201326787624
pineapple by-product and canola oil as partial fat replacers in low-fat beef burger: effects on oxidative stability, cholesterol content and fatty acid profile.the effect of freeze-dried pineapple by-product and canola oil as fat replacers on the oxidative stability, cholesterol content and fatty acid profile of low-fat beef burgers was evaluated. five treatments were performed: conventional (cn, 20% fat) and four low-fat formulations (10% fat): control (ct), pineapple by-product (pa), canola oil (co), and pineapple by-product and canola oil (pc). low-fat cooked burgers showed a mean cholesterol content reduction of 9.15% compared to the cn. canola oil ...201626775152
leaf malate and succinate accumulation are out of phase throughout the development of the cam plant ananas comosus.in plants with crassulacean acid metabolism (cam), organic acids, mainly malate are crucial intermediates for carbon fixation. in this research we studied the circadian oscillations of three organic anions (malate, citrate, and succinate) in ananas comosus, assessing the effect of season and plant development stage. seasonal and plant development dependencies were observed. the circadian oscillations of malate and citrate were typical of cam pathways reported in the literature. citrate content w ...201626773544
alteration of porcine serum albumin levels in pork meat by marination in kiwi or pineapple juice and subsequent pan broiling.this study was conducted to evaluate the changes in porcine serum albumin (psa), a major allergen, which occur when raw pork ham is marinated with kiwi or pineapple juice, and/or when the ham is pan broiled at 300℃ for 4 min after marination. in this study, raw pork ham was soaked for 4 h or 8 h in marinades containing commercial marinating sauce only, commercial marinating sauce and 7% kiwi juice, or commercial marinating sauce and 7% pineapple juice. when the meat was marinated and then pan-br ...201426761177
dietary patterns and retinal vascular calibre in children and adolescents with type 1 diabetes.to examine the association between dietary patterns and retinal vascular calibre in children and adolescents with type 1 diabetes.201626749006
novel species and expanded distribution of ellipsoidal multicellular magnetotactic prokaryotes.multicellular magnetotactic prokaryotes (mmps) are a peculiar group of magnetotactic bacteria, each comprising approximately 10-100 cells of the same phylotype. two morphotypes of mmp have been identified, including several species of globally distributed spherical mulberry-like mmps (s-mmps), and two species of ellipsoidal pineapple-like mmps (e-mmps) from china (qingdao and rongcheng cities). we recently collected e-mmps from mediterranean sea sediments (six-fours-les-plages) and drummond isla ...201626711721
erratum to: complete chloroplast genome sequence of md-2 pineapple and its comparative analysis among nine other plants from the subclass commelinidae. 201526683477
pain in the early phase of pediatric pancreatitis (pineapple trial): pre-study protocol of a multinational prospective clinical trial.there are unexpectedly large differences between the incidences of acute pancreatitis (ap) as indicated by different hospitals. retrospective studies suggest that the reason behind this is the large differences that exist between the local managements of abdominal pain at emergency units. unfortunately, no evidence-based medicine (ebm) guidelines are available to give proper instruction concerning the necessity of serum pancreatic enzyme measurement during abdominal pain.201626641250
developing single nucleotide polymorphism markers for the identification of pineapple (ananas comosus) germplasm.pineapple (ananas comosus [l.] merr.) is the third most important tropical fruit in the world after banana and mango. as a crop with vegetative propagation, genetic redundancy is a major challenge for efficient genebank management and in breeding. using expressed sequence tag and nucleotide sequences from public databases, we developed 213 single nucleotide polymorphism (snp) markers and validated 96 snps by genotyping the united states department of agriculture - agricultural research service p ...201526640697
cultivar identification and genetic relationship of pineapple (ananas comosus) cultivars using ssr markers.the genetic relationships among 27 pineapple [ananas comosus (l.) merr.] cultivars and lines were examined using 16 simple sequence repeat (ssr) markers. the number of alleles per locus of the ssr markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. similarity coefficients were calculated on the basis of 51 amplified bands. a dendrogram was created according to the 16 ssr markers by the unweighted pair-group method. the banding patterns obtained from the ssr primers allowed most ...201526634465
[basic studies on the stability of flavored oral solutions of rebamipide].stomatitis frequently occurs during chemotherapy and radiotherapy for cancer. because of its pharmacological properties including anti-inflammatory activity and stimulatory effects on endogenous prostaglandin synthesis, rebamipide has been suggested as a potentially effective treatment against stomatitis. in the present study we tested the stability of oral rebamipide solutions prepared in our hospital pharmacy using sodium alginate as a thickener to increase retention of this agent in the oral ...201526632156
a taste of pineapple evolution through genome sequencing.the genome sequence assembly of the highly heterozygous ananas comosus and its varieties is an impressive technical achievement. the sequence opens the door to a greater understanding of pineapple morphology and evolution.201526620110
reverse micellar extraction of bromelain from pineapple peel--effect of surfactant structure.pineapple peel is generally disposed or used as compost. this study was focused on extracting bromelain from pineapple peel by using reverse micelles. it was found that gemini surfactant c12-8-c12·2br (octamethylene-α,ω-bis(dimethyldodecylammonium bromide)) showed distinctive advantage over its monomeric counterpart dtab (dodecyl trimethyl ammonium bromide); under optimized condition, the bromelain extracted with c12-8-c12·2br reverse micelle had an activity recovery of 163% and a purification f ...201626616974
trade-offs of flowering and maturity synchronisation for pineapple quality.in the pineapple sector of benin, poor fruit quality prevents pineapple producers to enter the european market. we investigated effects of common cultural practices, flowering and maturity synchronisation, (1) to quantify the trade-offs of flowering and maturity synchronisation for pineapple quality and the proportion of fruits exportable to european markets, and (2) to determine the effect of harvesting practice on quality attributes. four on-farm experiments were conducted during three years u ...201526599545
ethanol determination in frozen fruit pulps: an application of quantitative nuclear magnetic resonance.this study reports the chemical composition of five types of industrial frozen fruit pulps (acerola, cashew, grape, passion fruit and pineapple fruit pulps) and compares them with homemade pulps at two different stages of ripening. the fruit pulps were characterized by analyzing their metabolic profiles and determining their ethanol content using quantitative nuclear magnetic resonance (qnmr). in addition, principal component analysis (pca) was applied to extract more information from the nmr da ...201626578064
discovery of a sexual cycle in talaromyces amestolkiae.talaromyces amestolkiae is a common cosmopolitan species that has been cultured from indoor house dust, sputum and lungs from cystic fibrosis patients, indoor air, wheat, soil, pineapple, sculptures and manure. it was described as an asexual talaromyces species and was reported to produce black sclerotia. in this study we report on the induction of sexual reproductive structures in t. amestolkiae. the mating type of 18 t. amestolkiae strains was determined with mat-specific primers. subsequently ...201626577610
long-term preconditioning of plantlets: a practical method for enhancing survival of pineapple (ananas comosus merr.) shoot tips cryopreserved using vitrification.the purpose of this study was to develop an efficient cryopreservation protocol for pineapple (ananas comosus merr.) shoot tips.201526575997
effects of pineapple byproduct and canola oil as fat replacers on physicochemical and sensory qualities of low-fat beef burger.pineapple byproduct and canola oil were evaluated as fat replacers on physicochemical and sensory characteristics of low-fat burgers. five treatments were performed: conventional (cn, 20% fat) and four low-fat formulations (10% fat): control (ct), pineapple byproduct (pa), canola oil (co), pineapple byproduct and canola oil (pc). higher water and fat retention and lower cooking loss and diameter reduction were found in burgers with byproduct addition. in raw burgers, byproduct incorporation redu ...201626562792
the pineapple genome and the evolution of cam photosynthesis.pineapple (ananas comosus (l.) merr.) is the most economically valuable crop possessing crassulacean acid metabolism (cam), a photosynthetic carbon assimilation pathway with high water-use efficiency, and the second most important tropical fruit. we sequenced the genomes of pineapple varieties f153 and md2 and a wild pineapple relative, ananas bracteatus accession cb5. the pineapple genome has one fewer ancient whole-genome duplication event than sequenced grass genomes and a conserved karyotype ...201526523774
stability, purification, and applications of bromelain: a review.bromelain is a cysteine protease found in pineapple tissue. because of its anti-inflammatory and anti-cancer activities, as well as its ability to induce apoptotic cell death, bromelain has proved useful in several therapeutic areas. the market for this protease is growing, and several studies exploring various properties of this molecule have been reported. this review aims to compile this data, and summarize the main findings on bromelain in the literature to date. the physicochemical properti ...201626518672
square bananas, blue horses: the relative weight of shape and color in concept recognition and representation.the present study investigates the role that shape and color play in the representation of animate (i.e., animals) and inanimate manipulable entities (i.e., fruits), and how the importance of these features is modulated by different tasks. across three experiments participants were shown either images of entities (e.g., a sheep or a pineapple) or images of the same entities modified in color (e.g., a blue pineapple) or in shape (e.g., an elongated pineapple). in experiment 1 we asked participant ...201526500593
antibacterial activity of papain and bromelain on alicyclobacillus spp.alicyclobacillus spp. are spore forming bacteria that are often related to the deterioration of acidic products such as beverages and citrus juices. after the process of industrial pasteurization, the spore produced by the bacteria can germinate and the microorganism can grow, causing sensory abnormalities in the product. alternative biopreservatives, such as the antimicrobial compounds, are of considerable importance to the food industry. papain and bromelain are proteolytic enzymes derived fro ...201626476327
independent origins and incipient speciation among host-associated populations of thielaviopsis ethacetica in cameroon.thielaviopsis ethacetica was recently reinstated as a distinct taxon using dna phylogenies. it is widespread affecting several crop plants of global economic importance. in this study, microsatellite markers were developed and used in conjunction with sequence data to investigate the genetic diversity and structure of th. ethacetica in cameroon. a collection of 71 isolates from cacao, oil palm, and pineapple, supplemented with nine isolates from other countries were analysed. four genetic groups ...201526466872
modulation of lipopolysaccharide stimulated nuclear factor kappa b mediated inos/no production by bromelain in rat primary microglial cells.microglial cells act as the sentinel of the central nervous system .they are involved in neuroprotection but are highly implicated in neurodegeneration of the aging brain. when over-activated, microglia release pro-inflammatory factors, such as nitric oxide (no) and cytokines, which are critical in eliciting neuroinflammatory responses associated with neurodegenerative diseases. this study examined whether bromelain, the pineapple-derived extract, may exert an anti-inflammatory effect in primary ...201626459398
biology and trapping of stable flies (diptera: muscidae) developing in pineapple residues (ananas comosus) in costa rica.pineapple production in costa rica increased nearly 300-fold during the last 30 yr, and >40,000 hectares of land are currently dedicated to this crop. at the end of the pineapple cropping cycle, plants are chopped and residues incorporated into the soil in preparation for replanting. associated with increased pineapple production has been a large increase in stable fly, stomoxys calcitrans (l.), populations. stable flies are attracted to, and oviposit in, the decomposing, chopped pineapple resid ...201526454479
agave as a model cam crop system for a warming and drying world.as climate change leads to drier and warmer conditions in semi-arid regions, growing resource-intensive c3 and c4 crops will become more challenging. such crops will be subjected to increased frequency and intensity of drought and heat stress. however, agaves, even more than pineapple (ananas comosus) and prickly pear (opuntia ficus-indica and related species), typify highly productive plants that will respond favorably to global warming, both in natural and cultivated settings. with nearly 200 ...201526442005
isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico.mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ...201526405529
iron migration from undamaged and dented juice tinplate cans.iron migration from tinplate cans to pineapple drink was studied over time using flame atomic absorption spectroscopy, taking into consideration storage temperature, sell-by date and can condition (dented/undamaged). an organoleptic test, at the sell-by date, was also performed.201626402218
an electrochemical impedance spectroscopy-based technique to identify and quantify fermentable sugars in pineapple waste valorization for bioethanol production.electrochemical impedance spectroscopy (eis) has been used to develop a methodology able to identify and quantify fermentable sugars present in the enzymatic hydrolysis phase of second-generation bioethanol production from pineapple waste. thus, a low-cost non-destructive system consisting of a stainless double needle electrode associated to an electronic equipment that allows the implementation of eis was developed. in order to validate the system, different concentrations of glucose, fructose ...201526378537
plasticity of crassulacean acid metabolism at subtropical latitudes: a pineapple case study.plants with the crassulacean acid metabolism (cam) express high-metabolic plasticity, to adjust to environmental stresses. this article hypothesizes that irradiance and nocturnal temperatures are the major limitations for cam at higher latitudes such as the azores (37°45'n). circadian cam expression in ananas comosus l. merr. (pineapple) was assessed by the diurnal pattern of leaf carbon fixation into l-malate at the solstices and equinoxes, and confirmed by determining maximal phosphoenolpyruva ...201626362993
phylogeography of pink pineapple mealybugs, dysmicoccus brevipes (cockerell) reveals the history of pineapple introduction and cultivation in china.the pink pineapple mealybug (ppm), dysmicoccus brevipes (cockerell) (hemiptera: pseudococcidae), is a widespread plant-sucking insect of considerable concern because it transmits the pineapple mealybug wilt-associated virus. its distribution is closely linked with its host, the pineapple [ananas comosus (l.) merrill] because of its wingless and parthenogenetic characteristics. to investigate the history of d. brevipes introduction and the cultivation of pineapple in china, samples of d. brevipes ...201526345923
kinetics studies with fruit bromelain (ananas comosus) in the presence of cysteine and divalent ions.the kinetics of cysteine and divalent ion modulation viz. ca(2+), cu(2+), hg(2+) of fruit bromelain (ec 3.4.22.33) have been investigated in the present study. kinetic studies revealed that at ph 4.5, cysteine induced v-type activation of bromelain catalyzed gelatin hydrolysis. at ph 3.5, ca(2+) inhibited the enzyme noncompetitively, whereas, both k-and v-type activations of bromelain were observed in the presence of 0.5 mm ca(2+) at ph 4.5 and 7.5. bromelain was inhibited competitively at 0.6 m ...201526345013
molecularly imprinted calixarene fiber for solid-phase microextraction of four organophosphorous pesticides in fruits.calixarene was used as a functional monomer to fabricate a molecularly imprinted polymer (mip) by sol-gel technique for solid-phase microextraction (spme) of parathion-methyl and its structural analogs. the mip-coated fiber possessed excellent thermal and chemical stability as well as high extraction capacity. its selectivity and possible recognition mechanism were investigated. the similarities in molecular shape and functional group play a key role in the selective recognition of the imprinted ...201626304345
evaluating agro-industrial by-products as dietary roughage source on growth performance of fattening steers.silages from pineapple peel, sweet corn husk and cob mixed with bagasse and vinasse were evaluated to determine their chemical composition and fermentation characteristics as well as feeding performance in fattening steers. the experiment, which lasted 90 days, involved 48 fattening steers (264 ± 37.4 kg bw) randomly allocated to three diets. treatments included: a control diet containing rice straw and molasses (t1); diet containing bagasse-vinasse mixture including sweet corn husk and cob sila ...201526288562
poly(n-isopropylacrylamide)-co-acrylamide hydrogels for the controlled release of bromelain from agroindustrial residues of ananas comosus.this works reports the purification of bromelain extracted from ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(n-isopropylacrylamide)-co-acrylamide hydrogels. bromelain was recovered within the 30-70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. the resulting purified bromelain contained ...201526287766
complete chloroplast genome sequence of md-2 pineapple and its comparative analysis among nine other plants from the subclass commelinidae.pineapple (ananas comosus var. comosus) is known as the king of fruits for its crown and is the third most important tropical fruit after banana and citrus. the plant, which is indigenous to south america, is the most important species in the bromeliaceae family and is largely traded for fresh fruit consumption. here, we report the complete chloroplast sequence of the md-2 pineapple that was sequenced using the pacbio sequencing technology.201526264372
second generation bioethanol potential from selected malaysia's biodiversity biomasses: a review.rising global temperature, worsening air quality and drastic declining of fossil fuel reserve are the inevitable phenomena from the disorganized energy management. bioethanol is believed to clear out the effects as being an energy-derivable product sourced from renewable organic sources. second generation bioethanol interests many researches from its unique source of inedible biomass, and this paper presents the potential of several selected biomasses from malaysia case. as one of countries with ...201626253329
[efficacy of rebamipide gargle against chemotherapy-induced oral mucositis].chemotherapy-induced oral mucositis (ciom) is a severe adverse event resulting from cancer chemotherapy. toxic free radicals and pro-inflammatory cytokines produced by anticancer drugs have been reported to be associated with ciom. rebamipide has been shown to increase gastric endogenous prostaglandin e2 and i2, to promote gastric epithelial mucin, and to behave as an oxygen free-radical scavenger in addition to other anti-inflammatory actions. we developed a gargle solution of rebamipide, addin ...201526234350
the energetic characterization of pineapple crown leaves.energetic characterization of biomass allows for assessing its energy potential for application in different conversion processes into energy. the objective of this study is to physicochemically characterize pineapple crown leaves (pc) for their application in energy conversion processes. pc was characterized according to astm e871-82, e1755-01, and e873-82 for determination of moisture, ash, and volatile matter, respectively; the fixed carbon was calculated by difference. higher heating value w ...201526233737
response surface optimization of process parameters and fuzzy analysis of sensory data of high pressure-temperature treated pineapple puree.the high-pressure processing conditions were optimized for pineapple puree within the domain of 400-600 mpa, 40-60 °c, and 10-20 min using the response surface methodology (rsm). the target was to maximize the inactivation of polyphenoloxidase (ppo) along with a minimal loss in beneficial bromelain (brm) activity, ascorbic acid (aa) content, antioxidant capacity, and color in the sample. the optimum condition was 600 mpa, 50 °c, and 13 min, having the highest desirability of 0.604, which resulte ...201526220205
studies into the phenolic patterns of different tissues of pineapple (ananas comosus [l.] merr.) infructescence by hplc-dad-esi-ms (n) and gc-ms analysis.in a comprehensive study, more than 60 phenolic compounds were detected in methanolic extracts from different tissues of pineapple infructescence by high-performance liquid chromatography with diode array detection and electrospray ionisation multiple-stage mass spectrometry (hplc-dad-esi-ms (n) ) as well as by gas chromatography-mass spectrometry (gc-ms). the analytical workflow combining both methods revealed numerous compounds assigned for the first time as pineapple constituents by their mas ...201526215283
empirical model based on weibull distribution describing the destruction kinetics of natural microbiota in pineapple (ananas comosus l.) puree during high-pressure processing.high pressure inactivation of natural microbiota viz. aerobic mesophiles (am), psychrotrophs (pc), yeasts and molds (ym), total coliforms (tc) and lactic acid bacteria (lab) in pineapple puree was studied within the experimental domain of 0.1-600 mpa and 30-50 °c with a treatment time up to 20 min. a complete destruction of yeasts and molds was obtained at 500 mpa/50 °c/15 min; whereas no counts were detected for tc and lab at 300 mpa/30 °c/15 min. a maximum of two log cycle reductions was obtai ...201526202323
a comparative study on the effect of conventional thermal pasteurisation, microwave and ultrasound treatments on the antioxidant activity of five fruit juices.a comparative study on the effect of conventional thermal pasteurisation, microwave and ultrasound treatments on the phytochemical and antioxidant activities of juices from carambola (averrhoa carambola l.), black jamun (syzygium cumuni l.skeels.), watermelon (citrullus lanatus var lanatus), pineapple (ananas comosus l. merr) and litchi (litchi chinensis sonn.) was carried out. depending on the type of fruit sample and treatment, increase or decrease in phytochemical values was observed. overall ...201626190045
interaction of polyphenol oxidase of solanum tuberosum with β-cyclodextrin: process details and applications.polysaccharides differing in structure and chemical nature were screened for their ability to bind non-covalently with polyphenol oxidase (ppo) from potato (as a model) and their effect on enzyme activity. all the polysaccharides selected inhibited the ppo but β-cyclodextrin showed maximum inhibition under optimum conditions. process details for the inhibition of ppo were studied with respect to concentration of β-cyclodextrin, temperature, ph, and time. higher inhibition constant and lower half ...201526187193
cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials.according to the cost of carbon substrate for producing biopolymer of polyhydroxyalkanoates (phas) still has a barrier to extend in an industrial scale. the aim of this work was to evaluate the defined phas media containing the agricultural raw materials of pineapple and sugarcane to produce phas by bacillus strain. batch fermentation was carried out in flask scale to compare the efficiency of defined media using statistical methodology. the defined medium 8 of pineapple was achieved the highest ...201526185927
leaf margin phenotype-specific restriction-site-associated dna-derived markers for pineapple (ananas comosus l.).to explore genome-wide dna polymorphisms and identify dna markers for leaf margin phenotypes, a restriction-site-associated dna sequencing analysis was employed to analyze three bulked dnas of f1 progeny from a cross between a 'piping-leaf-type' cultivar, 'yugafu', and a 'spiny-tip-leaf-type' variety, 'yonekura'. the parents were both ananas comosus var. comosus. from the analysis, piping-leaf and spiny-tip-leaf gene-specific restriction-site-associated dna sequencing tags were obtained and desi ...201526175625
diagnosis of gastroesophageal reflux disease using real-time magnetic resonance imaging.a small angle (his angle) between the oesophagus and the fundus of the stomach is considered to act as flap valve and anti-reflux barrier. a wide angle results in dysfunction of the oesophagogastric junction and subsequently in gastroesophageal reflux disease (gerd). here, we used real-time magnetic resonance imaging (mri) at 50 ms resolution (20 frames per second) in 12 volunteers and 12 patients with gerd to assess transport of pineapple juice through the oesophagogastric junction and reflux d ...201526175205
use of pressure for improving storage quality of fresh-cut produce.the microflora of fresh-cut produce is comprised primarily of phytopathogenic and soilborne organisms, but the product could be contaminated with foodborne pathogens. populations of bacteria, molds, and yeasts associated with fresh-cut produce decreased to non-detectable levels following a high pressure (hp) treatment of 400 mpa for 10 min at room temperature, except for spore-forming bacteria such as bacillus spp. which were inactivated when subjected to 600 mpa at 60 °c for 10 min. the hp trea ...201526174398
molecular cloning and characterization of udp-glucose: furaneol glucosyltransferase gene from grapevine cultivar muscat bailey a (vitis labrusca × v. vinifera).2,5-dimethyl-4-hydroxy-3(2h)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from american grape (vitis labrusca) and its hybrid grape. furaneol glucoside was recently isolated as an important furaneol derivative from the hybrid grapevine cultivar, muscat b ...201526160581
effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel.fruit by-products are good resources of carbohydrates, proteins, vitamins, and minerals, which may function as growth nutrients for probiotic bacteria. this research aimed at evaluating effects of pineapple peel powder addition on the viability and activity of lactobacillus acidophilus (atcc 4356), lactobacillus casei (atcc393), and lactobacillus paracasei ssp. paracasei (atcc baa52) in yogurts throughout storage at 4°c for 28d. plain and probiotic yogurts supplemented with or without pineapple ...201526142843
discovery of precursor and mature micrornas and their putative gene targets using high-throughput sequencing in pineapple (ananas comosus var. comosus).micrornas (mirnas) are a class of small, endogenous non-coding rnas that negatively regulate gene expression, resulting in the silencing of target mrna transcripts through mrna cleavage or translational inhibition. mirnas play significant roles in various biological and physiological processes in plants. however, the mirna-mediated gene regulatory network in pineapple, the model tropical non-climacteric fruit, remains largely unexplored. here, we report a complete list of pineapple mature mirnas ...201526115767
toward systems-level analysis of agricultural production from crassulacean acid metabolism (cam): scaling from cell to commercial production.systems-level analyses have become prominent tools for assessing the yield, viability, economic consequences and environmental impacts of agricultural production. such analyses are well-developed for many commodity crops that are used for food and biofuel, but have not been developed for agricultural production systems based on drought-tolerant plants that use crassulacean acid metabolism (cam). we review the components of systems-level evaluations, and identify the information available for com ...201526094655
fungal presence in selected tree nuts and dried fruits.sixty-four tree nut samples (almonds, pecans, pine nuts, and walnuts) and 50 dried fruit samples (apricots, cranberries, papaya, pineapple, and raisins) were purchased from local supermarkets and analyzed for fungal contamination using conventional culture as well as molecular methods. the results of our study showed that the highest yeast and mold (ym) counts (5.34 log10 cfu g(-1)) were found in walnuts and the lowest in pecans. the most common mold in nuts was aspergillus niger, relatively low ...201526056470
dengue viruses in aedes albopictus skuse from a pineapple plantation in costa rica. 201526047200
quality control of fruit juices by using organic acids determined by capillary zone electrophoresis with poly(vinyl alcohol)-coated bubble cell capillaries.an enhanced method for the determination of organic acids in several fruit juices by capillary zone electrophoresis (cze) with direct uv-vis detection has been developed in this work. first, a study with simulated real juice samples was done to find the best separation conditions. next, several commercial fruit juices were analyzed, and the organic acid contents were quantified in less than 12 min using a poly(vinyl alcohol)-coated fused-silica 'bubble cell' capillary. the present method is reli ...201526041236
fluctuation of diptera larvae in phytotelmata and relation with climate variation in west sumatra indonesia.research of fluctuations in diptera's larvae in phytotelmata had been conducted at three locations in west sumatra, indonesia; padang, bukittinggi and payakumbuh; which aimed to determine the number and fluctuations diptera larvae in phytotelmata. the results obtained; the highest number of individual larvae diptera in phytotelmata was 7109 aedes albopictus larvae (49.56%), followed by larvae of culex tritaeniorhynchus with 2409 individuals (16.80%). larvae fluctuated every month and tent to inc ...201426035947
efficacy of reverse micellar extracted fruit bromelain in meat tenderization.reverse micellar extraction (rme) was used for the separation and purification of bromelain from pineapple core and efficacy of rme purified bromelain (rmeb) in tenderization of beef meat was compared with that of commercial stem bromelain (csb). rme resulted in reasonably high bromelain activity recovery (85.0 %) and purification fold (4.0). reduction in meat toughness was higher in rmeb treated meat (52.1 %) compared to raw (control) and csb treated (26.7 %). significant increase in water hold ...201526028772
postharvest exogenous application of abscisic acid reduces internal browning in pineapple.internal browning (ib) is a postharvest physiological disorder causing economic losses in pineapple, but there is no effective control measure. in this study, postharvest application of 380 μm abscisic acid (aba) reduced ib incidence by 23.4-86.3% and maintained quality in pineapple fruit. aba reduced phenolic contents and polyphenol oxidase and phenylalanine ammonia lyase activities; increased catalase and peroxidase activities; and decreased o2(·-), h2o2, and malondialdehyde levels. this sugge ...201526007196
fruit peels support higher yield and superior quality bacterial cellulose production.fruit peels, also known as rinds or skins, are wastes readily available in large quantities. here, we have used pineapple (pa) and watermelon (wm) peels as substrates in the culture media (containing 5 % sucrose and 0.7 % ammonium sulfate) for production of bacterial cellulose (bc). the bacterial culture used in the study, komagataeibacter hansenii produced bc under static conditions as a pellicle at the air-liquid interface in standard hestrin and schramm (hs) medium. the yield obtained was ~3. ...201525957154
occurrence of cellulose-producing gluconacetobacter spp. in fruit samples and kombucha tea, and production of the biopolymer.cellulose producing bacteria were isolated from fruit samples and kombucha tea (a fermented beverage) using cuso4 solution in modified watanabe and yamanaka medium to inhibit yeasts and molds. six bacterial strains showing cellulose production were isolated and identified by 16s rrna gene sequencing as gluconacetobacter xylinus strain dfbt, ga. xylinus strain dfr-1, gluconobacter oxydans strain dfr-2, g. oxydans strain dfr-3, acetobacter orientalis strain dfr-4, and gluconacetobacter intermedius ...201525926011
application of an aqueous two-phase micellar system to extract bromelain from pineapple (ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations.bromelain is a set of proteolytic enzymes found in pineapple (ananas comosus) tissues such as stem, fruit and leaves. because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. the present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (atpms), using triton x-114 (tx-114) and mcilvaine buffer, in the absence and presence of electrolytes cacl2 and ...201525919128
dai nippon printing co., ltd medi·ca cc for enumeration of coliform bacteria.a ready-made dry medium method for coliform count, the medi·ca cc method, was compared to the violet red bile agar method (bacteriological analytical manual, chapter 4, enumeration of escherichia coli and the coliform bacteria, section g) for nine raw foods from four food categories: raw ground pork, raw lamb, raw ground chicken, raw tuna fillet, raw salmon fillet, raw shrimp, fresh peeled banana, fresh cut pineapple, and fresh cut apple. the 95% confidence interval for the mean difference betwe ...201525857880
applications of microencapsulated bifidobacterium longum with eleutherine americana in fresh milk tofu and pineapple juice.bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. microencapsulation of b. longum with eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. microencapsulated b. longum with the extract and oligosaccharides extract in the food products showed better sur ...201525854832
selective pruning in pineapple plants as means to reduce heterogeneity in fruit quality.heterogeneity in fruit quality (size and taste) is a major problem in pineapple production chains. the possibilities were investigated of reducing the heterogeneity in pineapple in the field by pruning slips on selected plants, in order to promote the fruit growth on these plants. slips are side shoots that develop just below the pineapple fruit during fruit development. two on-farm experiments were carried out in commercial fields in benin with a cultivar locally known as sugarloaf, to determin ...201525853027
experience shapes our odor perception but depends on the initial perceptual processing of the stimulus.the questions of whether configural and elemental perceptions are competitive or exclusive perceptual processes and whether they rely on independent or dependent mechanisms are poorly understood. to examine these questions, we modified perceptual experience through preexposure to mixed or single odors and measured the resulting variation in the levels of configural and elemental perception of target odor mixtures. we used target mixtures that were spontaneously processed in a configural or an el ...201525832188
electrodialytic removal of nitrate from pineapple juice: effect on selected physicochemical properties, amino acids, and aroma components of the juice.this study aimed at investigating the effect of nitrate removal from pineapple juice by electrodialysis (ed) on selected properties of the ed-treated juice. single-strength pineapple juice with reduced pulp content was treated by ed to reduce the nitrate concentration to 15, 10, or 5 ppm. after ed, the removed pulp was added to the ed-treated juice and its properties, including electrical conductivity, acidity, ph, total soluble solids (tss), color, amino acids, and selected aroma compounds, wer ...201525827307
structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities.pineapple has a delicious taste and good health benefits. bioactive polysaccharides are important components of pineapple that might contribute to its health benefits. since little structural information on these polysaccharides is currently available, the aim of this study was to investigate their structural characteristics and bioactivities.201525820875
phenotypic and molecular diversity of meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation.meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen candida guilliermondii, which causes about 2% of the human blood infections. this yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. the question if isolates from different sources are physiologically and genetically similar, or if the v ...201525791010
transcriptome sequence analysis of an ornamental plant, ananas comosus var. bracteatus, revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis.ananas comosus var. bracteatus (red pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. thus high-throughput transcriptome sequencing of ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies.201525769053
determination of the phenolic composition from brazilian tropical fruits by uhplc-ms/ms.although brazil is the third largest fruit producer in the world, several specimens consumed are not well studied from the chemical viewpoint, especially for quantitative analysis. for this reason and the crescent employment of mass spectrometry (ms) techniques in food science we selected twenty-two phenolic compounds with important biological activities and developed an ultra-high performance liquid chromatography tandem mass spectrometry (uhplc-ms/ms) method using electrospray (esi) in negativ ...201525766829
the effect of nanoparticle permeation on the bulk rheological properties of mucus from the small intestine.the effectiveness of delivering oral therapeutic peptides, proteins and nucleotides is often hindered by the protective mucus barrier that covers mucosal surfaces of the gastrointestinal (gi) tract. encapsulation of active pharmaceutical ingredients (api) in nanocarriers is a potential strategy to protect the cargo but they still have to pass the mucus barrier. decorating nanoparticles with proteolytic enzymes has been shown to increase the permeation through mucus. here we investigate the effec ...201525758122
analysis of pineapple mealybug wilt associated virus -1 and -2 for potential rna silencing suppressors and pathogenicity factors.higher plants use rna silencing to defend against viral infections. as a counter defense, plant viruses have evolved proteins that suppress rna silencing. mealybug wilt of pineapple (mwp), an important disease of pineapple, has been associated with at least three distinct viruses, pineapple mealybug wilt associated virus -1, -2, and -3 (pmwav-1, -2, and -3). selected open reading frames (orfs) of pmwav-1 and pmwav-2 were screened for their local and systemic suppressor activities in agrobacteriu ...201525751306
comparative study of pineapple juice as a negative oral contrast agent in magnetic resonance cholangiopancreatography.the aim of this study was to compare the image quality of magnetic resonance cholangiopancreatography (mrcp) using pineapple juice (pj) or ranitidine as negative oral contrast agents and no agent.201525738055
rapid differentiation of commercial juices and blends by using sugar profiles obtained by capillary zone electrophoresis with indirect uv detection.a method for the determination of sugars in several fruit juices and nectars by capillary zone electrophoresis with indirect uv-vis detection has been developed. under optimal conditions, commercial fruit juices and nectars from several fruits were analyzed, and the sugar and cyclamate contents were quantified in less than 6 min. a study for the detection of blends of high-value juices (orange and pineapple) with cheaper alternatives was also developed. for this purpose, different chemometric te ...201525719749
ripening-dependent metabolic changes in the volatiles of pineapple (ananas comosus (l.) merr.) fruit: i. characterization of pineapple aroma compounds by comprehensive two-dimensional gas chromatography-mass spectrometry.qualitative ripening-dependent changes of pineapple volatiles were studied via headspace solid-phase microextraction and analyzed by comprehensive two-dimensional gas chromatography quadrupole mass spectrometry (hs-spme-gc×gc-qms). early green-ripe stage, post-harvest ripened, and green-ripe fruits at the end of their commercial shelf-life were compared to air-freighted pineapples harvested at full maturity. in total, more than 290 volatiles could be identified by mass spectrometry and their lin ...201525701413
antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice.pineapple (ananas comosus) was demonstrated to be hepatoprotective. this study aims to investigate the reversing effects of pineapple vinegar on paracetamol-induced liver damage in murine model.201525699088
effect of processing on physicochemical composition, bioactive compounds and enzymatic activity of yellow mombin (spondias mombin l.) tropical juice.yellow mombin (spondias mombin, l.) is a tropical fruit that presents exotic taste and aroma, being source of carotenoids and phenolics compounds. it presents a good potential for processing, despite some restriction related with the presence of high amounts of peroxidase (pod) and pectinmethylesterase (pme) which can cause sensory changes in the product. this work addresses the evaluation of changes in pod and pme enzyme activity during the traditional industrial processing used to produce trop ...201525694737
genomic study of the absorption mechanism of p-coumaric acid and caffeic acid of extract of ananas comosus l. leaves.cardiac disease has emerged as the leading cause of death worldwide, and food rich in phenolic acids has drawn much attention as sources of active substances of hypolipidemic drug. ananas comosus l. (pineapple) is one of the most popular tropical and subtropical fruits. isolated from pineapple leaves, eal(extract of ananas comosus l. leaves) is rich in phenolic acids, such as p-coumaric acid, caffeic acid, and other phenolics, highly relevant to the putative cardiovascular-protective effects, wh ...201525678210
food allergy knowledge, perception of food allergy labeling, and level of dietary practice: a comparison between children with and without food allergy experience.the prevalence of food allergies in korean children aged 6 to 12 years increased from 10.9% in 1995 to 12.6% in 2012 according to nationwide population studies. treatment for food allergies is avoidance of allergenic-related foods and epinephrine auto-injector (epi) for accidental allergic reactions. this study compared knowledge and perception of food allergy labeling and dietary practices of students.201525671074
influence of weight and type of planting material on fruit quality and its heterogeneity in pineapple [ananas comosus (l.) merrill].cultural practices can affect the quality of pineapple fruits and its variation. the objectives of this study were to investigate (a) effects of weight class and type of planting material on fruit quality, heterogeneity in quality and proportion and yield of fruits meeting european export standards, and (b) the improvement in quality, proportion and yield of fruits meeting export standards when flowering was induced at optimum time. experiments were conducted in benin with cvs sugarloaf (a perol ...201425653659
ripening-dependent metabolic changes in the volatiles of pineapple (ananas comosus (l.) merr.) fruit: ii. multivariate statistical profiling of pineapple aroma compounds based on comprehensive two-dimensional gas chromatography-mass spectrometry.ripening-dependent changes of pineapple volatiles were studied in a nontargeted profiling analysis. volatiles were isolated via headspace solid phase microextraction and analyzed by comprehensive 2d gas chromatography and mass spectrometry (hs-spme-gc×gc-qms). profile patterns presented in the contour plots were evaluated applying image processing techniques and subsequent multivariate statistical data analysis. statistical methods comprised unsupervised hierarchical cluster analysis (hca) and p ...201525651901
study on evaluation of silage from pineapple (ananas comosus) fruit residue as livestock feed.pineapple is a commercially important fruit crop grown in asian and african countries. pineapple fruit residue (pfr) accounts for more than 65% of the processed fruits, and its disposal is a major problem due to its high moisture and sugar content predisposing it to fungal growth and spoilage. silage technique was adopted to address this problem, and the pfr silage was evaluated for its feeding value. it was observed that on 15th day, the ph of pfr silage was 4.2-4.3 and lactic acid content was ...201525633915
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