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microsatellite loci and genetic structure of artificial populations of cotesia flavipes (hymenoptera, braconidae).cotesia flavipes (cameron) is a parasitoid wasp used in the biological control of the sugarcane borer (diatraea saccharalis) (fabr., 1794). studies on the genetic diversity of c. flavipes are hampered by the lack of highly polymorphic molecular markers. in this report, a set of 11 microsatellite loci were developed from an enriched library of c. flavipes. four microsatellite loci were polymorphic and were screened in 212 c. flavipes individuals (183 females and 29 males) that were randomly sampl ...201627886334
biological pretreatment of sugarcane bagasse with basidiomycetes producing varied patterns of biodegradation.this work evaluated sugarcane bagasse pretreatment with wood-decay fungi, producing varied patterns of biodegradation. the overall mass balance of sugars released after pretreatment and enzymatic hydrolysis indicated that a selective white-rot was necessary to provide glucose yields similar to the ones observed from leading physico-chemical pretreatment technologies. the selective white-rot ceriporiopsis subvermispora was selective for lignin degradation in the lignocellulosic material, preserve ...201727875764
can commercial digital cameras be used as multispectral sensors? a crop monitoring test.the use of consumer digital cameras or webcams to characterize and monitor different features has become prevalent in various domains, especially in environmental applications. despite some promising results, such digital camera systems generally suffer from signal aberrations due to the on-board image processing systems and thus offer limited quantitative data acquisition capability. the objective of this study was to test a series of radiometric corrections having the potential to reduce radio ...200827873930
the comparison of digestibility of treated sugarcane tops silage by bacteria or whole microorganisms of holstein cow and buffalo rumen.the aim of this study was to evaluate the effects of adding sulfuric acid to sugarcane tops silage on rumen bacteria and whole rumen microorganisms (wrm) and compare the digestibility of sugarcane tops treated with different amount of urea, molasses and sulfuric acid between holstein cow and khouzestan buffalo. regardless of the type of the treatment, potential of gas production (b) by cow wrm (130.670 ml) was more than buffalo (104.060 ml) (p < 0.05), but the rate of gas production (c) by buffa ...201627872716
production of l(+)-lactic acid from acid pretreated sugarcane bagasse using bacillus coagulans dsm2314 in a simultaneous saccharification and fermentation strategy.sugars derived from lignocellulose-rich sugarcane bagasse can be used as feedstock for production of l(+)-lactic acid, a precursor for renewable bioplastics. in our research, acid-pretreated bagasse was hydrolysed with the enzyme cocktail gc220 and fermented by the moderate thermophilic bacterium bacillus coagulans dsm2314. saccharification and fermentation were performed simultaneously (ssf), adding acid-pretreated bagasse either in one batch or in two stages. ssf was performed at low enzyme do ...201627872661
the family 22 carbohydrate-binding module of bifunctional xylanase/β-glucanase xyn10e from paenibacillus curdlanolyticus b-6 has an important role in lignocellulose degradation.a newly isolated endo-β-1,4-xylanase (xyn10e) from paenibacillus curdlanolyticus b-6 has a modular structure consisting of a family 22 carbohydrate-binding module (cbm), a glycoside hydrolase (gh) family 10 catalytic domain, two fibronectin type iii (fn3) domains, and a family 3 cbm at the c-terminus. intact xyn10e (rxyn10e), cbm22-deleted xyn10e (x-cbm3), cbm3-deleted xyn10e (x-cbm22), and gh10 catalytic domain only (x-gh10) were expressed in escherichia coli. rxyn10e showed bifunctional degrad ...201727871388
crosstalk between sugarcane and a plant-growth promoting burkholderia species.bacterial species in the plant-beneficial-environmental clade of burkholderia represent a substantial component of rhizosphere microbes in many plant species. to better understand the molecular mechanisms of the interaction, we combined functional studies with high-resolution dual transcriptome analysis of sugarcane and root-associated diazotrophic burkholderia strain q208. we show that burkholderia q208 forms a biofilm at the root surface and suppresses the virulence factors that typically trig ...201627869215
an entomopathogenic bacterium strain, bacillus thuringiensis, as a biological control agent against the red palm weevil, rhynchophorus ferrugineus (coleoptera: curculionidae).the red palm weevil (rpw), rhynchophorus ferrugineus, is an invasive wood-boring insect that damages palms and sugarcane. bacillus thuringiensis (bt) is an entomopathogenic bacterium which has been modified into various strains and widely used in pest management. the aim of this study was to evaluate the susceptibility of rpw to the ha strain of bt.201627862867
possibly similar genetic basis of resistance to bacillus thuringiensis cry1ab protein in 3 resistant colonies of the sugarcane borer collected from louisiana, usa.the sugarcane borer, diatraea saccharalis (f.), is a major maize borer pest and a target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. evolution of resistance in target pest populations is a great threat to the long-term efficacy of bt crops. in this study, we compared the genetic basis of resistance to cry1ab protein in 3 resistant colonies of sugarcane borer established from field populations in louisiana, ...201627860288
production of poly(malic acid) from sugarcane juice in fermentation by aureobasidium pullulans: kinetics and process economics.poly(β-l-malic acid) (pma) is a biodegradable polymer with many potential biomedical applications. pma can be readily hydrolyzed to malic acid (ma), which is widely used as an acidulant in foods and pharmaceuticals. pma production from sucrose and sugarcane juice by aureobasidium pullulans zx-10 was studied in shake-flasks and bioreactors, confirming that sugarcane juice can be used as an economical substrate without any pretreatment or nutrients supplementation. a high pma titer of 116.3g/l and ...201727839861
insect and plant-derived mirnas in greenbug (schizaphis graminum) and yellow sugarcane aphid (sipha flava) revealed by deep sequencing.schizaphis graminum (green bug; gb) and sipha flava (yellow sugarcane aphid; ysa) are two cereal aphid species with broad host ranges capable of establishing on sorghum (sorghum bicolor) and several switchgrass (panicum virgatum) cultivars. switchgrass and sorghum are staple renewable bioenergy crops that are vulnerable to damage by aphids, therefore, identifying novel targets to control aphids has the potential to drastically improve yields and reduce losses in these bioenergy crops. despite th ...201727838454
transgenic sugarcane overexpressing canecpi-1 negatively affects the growth and development of the sugarcane weevil sphenophorus levis.transgenic sugarcane expressing canecpi-1 exhibits resistance to sphenophorus levis larvae. transgenic plants have widely been used to improve resistance against insect attack. sugarcane is an economically important crop; however, great losses are caused by insect attack. sphenophorus levis is a sugarcane weevil that digs tunnels in the stem base, leading to the destruction of the crop. this insect is controlled inefficiently by chemical insecticides. transgenic plants expressing peptidase inhib ...201727837261
ethanol production in brazil: a bridge between science and industry.in the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in brazil. these contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. better control of fermentation conditions can ...201627818090
identification and molecular characterization of a novel sugarcane streak mastrevirus and an isolate of the a-strain of maize streak virus from sugarcane in nigeria.sugarcane and maize plants showing symptoms typical of those described for the so-called "african streak viruses" (afsvs) were encountered during field surveys conducted from february to july 2015 to document viruses infecting both crops across the northern guinea savannah region of nigeria. as part of this study, two categories of complete mastrevirus-like genome sequences were obtained from nine samples (maize = 2; sugarcane = 7). in pairwise comparisons, the full-length genomes of the first s ...201727815694
use of sugarcane molasses by pycnoporus sanguineus for the production of laccase for dye decolorization.pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. we evaluated laccase production in a recently isolated strain of p. sanguineus cultivated with sugarcane molasses as the only carbon source, and urea or yeast extract as the nitrogen source ...201627813609
draft genome sequence of leifsonia xyli subsp. xyli strain gdw1.here, we report the draft genome sequence of leifsonia xyli subsp. xyli strain gdw1, isolated from the stem of badila sugarcane located at the guangdong key laboratory for crops genetic improvement (guanzhou, china), that causes ratoon stunting disease of sugarcane. the de novo genome of leifsonia xyli subsp. xyli was assembled with 48 scaffolds and a g+c content of 67.68%, and contained 2.6 mb bp and 2,838 coding sequences.201627795270
genome editing in sugarcane: challenges ahead.genome editing opens new and unique opportunities for researchers to enhance crop production. until 2013, the zinc finger nucleases (zfns) and transcription activator-like effector nucleases (talens) were the key tools used for genome editing applications. the advent of rna-guided engineered nucleases - the type ii clustered regularly interspaced short palindromic repeat (crispr)/cas9 (crispr-associated) system from streptococcus pyogenes holds great potential since it is simple, effective and m ...201627790238
composition, taste, aroma, and antioxidant activity of solidified noncentrifugal brown sugars prepared from whole stalk and separated pith of sugarcane (saccharum officinarum l.).in this study, 2 types of solidified noncentrifugal brown sugars (w-ncs and p-ncs) were prepared from the whole stalk and separated pith, respectively, of raw sugarcane (saccharum officinarum l.). these products were discriminated in terms of their quality attributes, including color, sugars and minerals composition, taste, aroma, and antioxidant activity. the brown color of p-ncs was clearly different compared with that of w-ncs with a color difference value (δe* ) of 9.36. there was no differe ...201627780296
landscape, environmental and social predictors of hantavirus risk in são paulo, brazil.hantavirus pulmonary syndrome (hps) is a disease caused by hantavirus, which are negative-sense rna viruses in the family bunyaviridae that are highly virulent to humans. numerous factors modify risk of hantavirus transmission and consequent hps risk. human-driven landscape change can foster transmission risk by increasing numbers of habitat generalist rodent species that serve as the principal reservoir host. climate can also affect rodent population dynamics and hantavirus survival, and a numb ...201627780250
identification of genes involved in indole-3-acetic acid biosynthesis by gluconacetobacter diazotrophicus pal5 strain using transposon mutagenesis.gluconacetobacter diazotrophicus is a beneficial nitrogen-fixing endophyte found in association with sugarcane plants and other important crops. beneficial effects of g. diazotrophicus on sugarcane growth and productivity have been attributed to biological nitrogen fixation process and production of phytohormones especially indole-3-acetic acid (iaa); however, information about the biosynthesis and function of iaa in g. diazotrophicus is still scarce. therefore, the aim of this work was to ident ...201627774087
histopathological analysis of tilapia gills (oreochromis niloticus linnaeus, 1758) exposed to sugarcane vinasse.sugarcane vinasse is one of the main residues generated by the transformation of cane into ethanol. because of the high organic content (cod), high biochemical oxygen demand (bod), low ph, the large amount that this residue is generated (15l for every liter of ethanol produced) and their use as fertilizer on the sugarcane crop, this residue is potentially polluting to the soil ecossystem and by percolation to water ecossystem too. thus, this study aimed to assess the toxicity of vinasse by analy ...201727770647
d-003 (saccharum officinarum): the forgotten lipid-lowering agent.reduction of elevated cholesterol levels, particularly low-density lipoprotein cholesterol (ldl-c), is essential in primary and secondary prevention of cardiovascular disease (cvd). therefore there is still a large need for new effective drugs, which would be able to essentially reduce ldl-c and in the consequence cv residual risk. d-003 is a mixture of high aliphatic primary acids purified from sugarcane (saccharum officinarum) wax. it showed promising hypocholesterolemic effects in both animal ...201627751878
optimization of levan production by cold-active bacillus licheniformis ant 179 and fructooligosaccharide synthesis by its levansucrase.fructooligosaccharides (fos) and levan attract much attention due to a wide range of applications in food technology and pharmaceutical and cosmetic industry. bacillus licheniformis ant 179, isolated from antarctica soil, produced levansucrase and levan in a medium containing sucrose as carbon substrate. in this study, characterization of levansucrase and production of short-chain fos and levan were investigated. temperature and ph optimum of the enzyme were found to be 60 °c and ph 6.0, respect ...201727734288
comparative proteomics reveals that central metabolism changes are associated with resistance against sporisorium scitamineum in sugarcane.sugarcane smut, which is caused by sporisorium scitamineum, has been threatening global sugarcane production. breeding smut resistant sugarcane varieties has been proven to be the most effective method of controlling this particular disease. however, a lack of genome information of sugarcane has hindered the development of genome-assisted resistance breeding programs. furthermore, the molecular basis of sugarcane response to s. scitamineum infection at the proteome level was incomplete and combi ...201627733120
rapid and quantitative detection of leifsonia xyli subsp. xyli in sugarcane stalk juice using a real-time fluorescent (taqman) pcr assay.ratoon stunting disease (rsd) of sugarcane, one of the most important diseases seriously affecting the productivity of sugarcane crops, was caused by the bacterial agent leifsonia xyli subsp. xyli (lxx). a taqman probe-based real-time quantitative polymerase chain reaction (qpcr) assay was established in this study for the quantification of lxx detection in sugarcane stalk juice. a pair of pcr primers (pat1-qf/pat1-qr) and a fluorogenic probe (pat1-qp) targeting the part1 gene of lxx were used f ...201627725937
expression of two novel β-glucosidases from chaetomium atrobrunneum in trichoderma reesei and characterization of the heterologous protein products.two novel gh3 family thermostable β-glucosidases from the filamentous fungus chaetomium atrobrunneum (cel3a and cel3b) were expressed in trichoderma reesei, purified by two-step ion exchange chromatography, and characterized. both enzymes were active over a wide range of ph as compared to neurospora crassa β-glucosidase gh3-3, which was also expressed in t. reesei and purified. the optimum temperature of both c. atrobrunneum enzymes was around 60 °c at ph 5, and both enzymes had better thermal a ...201627714589
magnesium ions in yeast: setting free the metabolism from glucose catabolite repression.in a recent work we showed that magnesium (mg(ii)) plays an important role in industrial ethanol production, overcoming the negative effect of the excess of minerals, particularly copper, present in sugarcane juice, with a consequent increase in ethanol yield. this cation has been reported to be involved in several steps of yeast metabolism, acting mainly as a co-factor of several enzymes of fermentation metabolism and protecting yeast cells from stressful conditions. however, despite many physi ...201627714092
comprehensive analysis of the cellulolytic system reveals its potential for deconstruction of lignocellulosic biomass in a novel streptomyces sp.the giant snail achatina fulica is considered an invasive species in most territories in which it was introduced, due to its ability to process a large amount of lignocellulose as a consequence of the presence of a cellulolytic-associated microflora. streptomyces are well known as crucial agents in the decomposition of complex polymers in soil environments and also as cellulolytic symbionts commonly associated with herbivore insects. here, we employed a combination of genomic and biochemical too ...201727699477
optimization of substrate preparation for oyster mushroom (pleurotus ostreatus) cultivation by studying different raw materials and substrate preparation conditions (composting: phases i and ii).in recent years, oyster mushroom (pleurotus ostreatus) has become one of the most cultivated mushrooms in the world, mainly in brazil. among many factors involved in a mushroom production, substrate preparation is the most critical step, which can be influenced by composting management techniques. looking forward to optimizing the substrate preparation process, were tested different composting conditions (7 and 14 days of composting with or without conditioning), potential raw materials (decumbe ...201627696289
biosynthesis, purification and characterization of β-1,4-xylanase from a novel mangrove associated actinobacterium streptomyces olivaceus (msu3) and its applications.the present study was undertaken to evaluate the xylanolytic properties of an actinobacterium streptomyces olivaceus (msu3) isolated from the sediment sample of mangrove environment. it showed highest xylanase activity on initial screening in breg's mineral salts medium supplemented with 0.5% xylan. further the organism expressed maximum xylanase production at optimized culture conditions of ph 7, temperature 30 °c with 2.5% of inoculum size at 72 h of incubation, the nutrient sources like sucro ...201727693622
phosphate solubilization potential of rhizosphere fungi isolated from plants in jimma zone, southwest ethiopia.phosphorus (p) is one of the major bioelements limiting agricultural production. phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. the present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya (pvk) medium. a total of 359 fungal isolates were obtained from 150 rhizosphere soil samples of haricot bean, faba bean, cabbage, tomato, and suga ...201627688771
d-xylose fermentation, xylitol production and xylanase activities by seven new species of sugiyamaella.sixteen yeast isolates identified as belonging to the genus sugiyamaella were studied in relation to d-xylose fermentation, xylitol production, and xylanase activities. the yeasts were recovered from rotting wood and sugarcane bagasse samples in different brazilian regions. sequence analyses of the internal transcribed spacer (its) region and the d1/d2 domains of large subunit rrna gene showed that these isolates belong to seven new species. the species are described here as sugiyamaella ayubii ...201727688209
biochemical compositions and fatty acid profiles in four species of microalgae cultivated on household sewage and agro-industrial residues.the potential of four regional microalgae species was evaluated in relation to their cell growth and biomass production when cultured in the following alternative media: bio-composts of fruit/horticultural wastes (hb), sugarcane waste and vinasse (vb) chicken excrements (bce), raw chicken manure (rcm), and municipal domestic sewage (mds). the cultures were maintained under controlled conditions and their growth responses, productivities, biochemical compositions, and the ester profiles of their ...201627668876
isolation and characterization of scglud2, a new sugarcane beta-1,3-glucanase d family gene induced by sporisorium scitamineum, aba, h2o2, nacl, and cdcl2 stresses.beta-1,3-glucanases (ec 3.2.1.39), commonly known as pathogenesis-related (pr) proteins, play an important role not only in plant defense against fungal pathogens but also in plant physiological and developmental processes. however, only a limited number of sugarcane beta-1,3-glucanase genes have been isolated. in the present study, we identified and characterized a new beta-1,3-glucanase gene scglud2 (genbank acc no. kf664181) from sugarcane. an x8 domain was present at the c terminal region of ...201627642288
soybean protein as a cost-effective lignin-blocking additive for the saccharification of sugarcane bagasse.addition of surfactants, polymers, and non-catalytic proteins can improve the enzymatic hydrolysis of lignocellulosic materials by blocking the exposed lignin surfaces, but involves extra expense. here, soybean protein, one of the cheapest proteins available, was evaluated as an alternative additive for the enzymatic hydrolysis of pretreated sugarcane bagasse. the effect of the enzyme source was investigated using enzymatic cocktails from a. niger and t. reesei cultivated under solid-state, subm ...201627639236
iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences.iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ...201727632283
predicting hotspots of human-elephant conflict to inform mitigation strategies in xishuangbanna, southwest china.research on the spatial patterns of human-wildlife conflict is fundamental to understanding the mechanisms underlying it and to identifying opportunities for mitigation. in the state of xishuangbanna, containing china's largest tropical forest, an imbalance between nature conservation and economic development has led to increasing conflicts between humans and asian elephants (elephas maximus), as both elephant numbers and conversion of habitable land to rubber plantations have increased over the ...201627631976
addition of surfactants and non-hydrolytic proteins and their influence on enzymatic hydrolysis of pretreated sugarcane bagasse.poly(ethylene glycol) (peg 4000) and bovine serum albumin (bsa) were investigated with the purpose of evaluating their influence on enzymatic hydrolysis of sugarcane bagasse. effects of these supplements were assayed for different enzymatic cocktails (trichoderma harzianum and penicillium funiculosum) that acted on lignocellulosic material submitted to different pretreatment methods with varying solid (25 and 100 g/l) and protein (7.5 and 20 mg/g cellulose) loadings. the highest levels of glucos ...201727631122
liming in the sugarcane burnt system and the green harvest practice affect soil bacterial community in northeastern são paulo, brazil.here we show that both liming the burnt sugarcane and the green harvest practice alter bacterial community structure, diversity and composition in sugarcane fields in northeastern são paulo state, brazil. terminal restriction fragment length polymorphism fingerprinting and 16s rrna gene cloning and sequencing were used to analyze changes in soil bacterial communities. the field experiment consisted of sugarcane-cultivated soils under different regimes: green sugarcane (gs), burnt sugarcane (bs), ...201627629424
decomposition rate and enzymatic activity of composted municipal waste and poultry manure in the soil in a biofuel crops field.biofuel crops are gaining importance because of the need to replace non-renewable sources. also, due to the increasing amounts of wastes generated, there is the need to recycle them to the soil, both to fertilize crops and to improve soil physical properties through organic matter increase and microbiological changes in the rhizosphere. we therefore studied the influence of six biofuel crops (elephant grass, giant cane, sugarcane, blue gum, black cottonwood, willow) on the decomposition rate and ...201727620351
bacterial abilities and adaptation toward the rhizosphere colonization.the rhizosphere harbors one of the most complex, diverse, and active plant-associated microbial communities. this community can be recruited by the plant host to either supply it with nutrients or to help in the survival under stressful conditions. although selection for the rhizosphere community is evident, the specific bacterial traits that make them able to colonize this environment are still poorly understood. thus, here we used a combination of community level physiological profile (clpp) a ...201627610108
morphogenic regulators baby boom and wuschel improve monocot transformation.while transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. here, we report a transformation approach involving overexpression of the maize (zea mays) baby boom (bbm) and maize wuschel2 (wus2) genes, which produced high transformation frequencies in numerous previously non-transformable maize i ...201627600536
phenolic compounds and anticancer activity of commercial sugarcane cultivated in brazil.the cultivation of sugarcane hybrids (x saccharum officinarum l.) is an important revenue source for the brazilian economy. herein it is reported the evaluation of the cytotoxic activity of mid-polarity sugarcane extracts against human cancer cell lines, as well as the isolation of steroids sitosterol, stigmasterol and campesterol, phenolic acids p-hydroxybenzoic, p-hydroxycinnamic, vanillic and ferulic acid, terpenoids α-tocopherol and β-carotene and a novel substance in sugarcane, the flavonoi ...201627598841
sugarcane serine peptidase inhibitors, serine peptidases, and clp protease system subunits associated with sugarcane borer (diatraea saccharalis) herbivory and wounding.sugarcane's (saccharum spp.) response to diatraea saccharalis (f.) (lepidoptera: (crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane expressed sequence tags (ests) encoding serine peptidase inhibitors, serine peptidases. and clp protease system subunits. our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. among the upregulated genes, nine were similar to serine peptidase inhibitors and four were ...201627598134
essential genes for in vitro growth of the endophyte herbaspirillum seropedicae smr1 as revealed by transposon insertion site sequencing.the interior of plants contains microorganisms (referred to as endophytes) that are distinct from those present at the root surface or in the surrounding soil. herbaspirillum seropedicae strain smr1, belonging to the betaproteobacteria, is an endophyte that colonizes crops, including rice, maize, sugarcane, and sorghum. different approaches have revealed genes and pathways regulated during the interactions of h. seropedicae with its plant hosts. however, functional genomic analysis of transposon ...201627590816
rnaseq transcriptional profiling following whip development in sugarcane smut disease.sugarcane smut disease is caused by the biotrophic fungus sporisorium scitamineum. the disease is characterized by the development of a whip-like structure from the primary meristems, where billions of teliospores are produced. sugarcane smut also causes tillering and low sucrose and high fiber contents, reducing cane productivity. we investigated the biological events contributing to disease symptoms in a smut intermediate-resistant sugarcane genotype by examining the transcriptional profiles ( ...201627583836
production of d-lactate from sugarcane bagasse and corn stover hydrolysates using metabolic engineered escherichia coli strains.in this study, the lactogenic escherichia coli strain ju15 was used and modified to produce d-lactate (d-la) from plant hydrolysates with a minimal nutrient addition in ph controlled fermenters. results showed that strain ju15 produces d-la with high yield and productivity in laboratory simulated hydrolysate media and actual sugar cane bagasse hemicellulosic hydrolysate. strain ju15 showed sequential carbon source utilization and acetic acid production. the l-lactic and acetic acid production pa ...201627573474
restriction site associated dna (rad) for de novo sequencing and marker discovery in sugarcane borer, diatraea saccharalis fab. (lepidoptera: crambidae).we present the development of a genomic library using radseq (restriction site associated dna sequencing) protocol for marker discovery that can be applied on evolutionary studies of the sugarcane borer diatraea saccharalis, an important south american insect pest. a radtag protocol combined with illumina paired-end sequencing allowed de novo discovery of 12 811 snps and a high-quality assembly of 122.8m paired-end reads from six individuals, representing 40 gb of sequencing data. approximately ...201627571734
screening of yeasts for selection of potential strains and their utilization for in situ microbial detoxification (ismd) of sugarcane bagasse hemicellulosic hydrolysate.many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. the conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. hence, it is imperative to explore biological methods to overcome the inhibitors by ...201627570309
recent trends in bioethanol production from food processing byproducts.the widespread use of corn starch and sugarcane as sources of sugar for the production of ethanol via fermentation may negatively impact the use of farmland for production of food. thus, alternative sources of fermentable sugars, particularly from lignocellulosic sources, have been extensively investigated. another source of fermentable sugars with substantial potential for ethanol production is the waste from the food growing and processing industry. reviewed here is the use of waste from potat ...201627565674
production of mannitol from a high concentration of glucose by candida parapsilosis sk26.001.a novel strain, sk26.001, which can produce mannitol from a high concentration of glucose without the addition of fructose, was isolated from sugarcane juice. this strain was identified as candida parapsilosis based on 18s ribosomal rna (rrna) sequence analysis and the morphological and physiological-biochemical characteristics of the strain. under optimized fermentation conditions, the mannitol concentration in shake flasks reached 68.5 g/l. when batch fermentation was performed, the fed glucos ...201727557902
striga biocontrol on a toothpick: a readily deployable and inexpensive method for smallholder farmers.striga hermonthica (witchweed) is a parasitic weed that attacks and significantly reduces the yields of maize, sorghum, millet, and sugarcane throughout sub-saharan africa. low cost management methods such as hand weeding, short crop rotations, trap cropping, or conventional biocontrol have not been effective. likewise, striga-tolerant or herbicide-resistant maize cultivars are higher yielding, but are often beyond the economic means of sustenance farmers. the fungal pathogen, fusarium oxysporum ...201627551284
influence of vinasse application in the structure and composition of the bacterial community of the soil under sugarcane cultivation.although the use of vinasse as a waste helps replenish soil nutrients and improves the quality of the sugarcane crop, it is known that vinasse residues alter the diversity of bacteria naturally present in the soil. the actual impacts of vinasse application on the selection of bacterial taxa are not understood because no studies have addressed this phenomenon directly. analysis of 16s rrna gene clone sequences from four soil types showed that the soil planted with sugarcane and fertilized with vi ...201627528875
antibacterial activity and phytochemical evidence for the plant origin of turkish propolis from different regions.honeybees collect propolis from practically any abundant plant source in the neighborhood of the hive, be it populus, eucalyptus, pine, sugarcane, cashew nut or orange trees. we have described that the origin plants of turkish propolis are populus sp., eucalyptus sp. and castanea sativa. in our previous study, propolis samples from middle anatolia displayed the typical pattern of "poplar" propolis: they contained pinobanksin, caffeic and ferulic acids and their esters. the propolis samples exami ...200727517836
synthesis of cellulose acetate and carboxymethylcellulose from sugarcane straw.sugarcane straw (scs) is a raw material with high potential for production of cellulose derivatives due to its morphology and structure. the proposal of this work was to synthesize cellulose acetate (ca) and carboxymethylcellulose (cmc) from sugarcane straw cellulose, and applied the ca in the preparation of a membrane. the cellulose extraction was carried out in four steps. firstly, scs was treated with h2so4 (10% v/v) followed by naoh (5% w/v) treatment. subsequently, a chelating process was p ...201627516319
quality of the exotic parasitoid cotesia flavipes (hymenoptera: braconidae) does not show deleterious effects after inbreeding for 10 generations.although the parasitoid cotesia flavipes (cameron) has proven effective in controlling sugarcane borer diatraea saccharalis (fabricius) for many years, concern has arisen over the quality of individuals produced at large scales. the parasitoid has been reared in laboratories in brazil for more than 40 years, with no new introductions of new populations during that period. since the quality of the parasitoids was not verified at the time of the species' introduction in brazil, we do not know if t ...201627509087
mapping n-linked glycosylation of carbohydrate-active enzymes in the secretome of aspergillus nidulans grown on lignocellulose.the genus aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (cazymes) that characterize their saprophyte lifestyle. aspergillus has the capacity to perform post-translational modifications (ptm), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins. in this study, the n-linked glycosylation of cazymes identified in the secretome of asperg ...201627508003
effects of genotype, wilting, and additives on the nutritive value and fermentation of bermudagrass silage.bermudagrass is the main warm-season grass species used for livestock production in the southeastern united states; however, when it is ensiled, the silage fermentation parameters are often less than desirable. the objective of this study was to investigate the effects of management practices on the nutritive value and fermentation characteristics of bermudagrass silage. in exp. 1, treatments were the factorial combinations of 2 bermudagrass genotypes, 'jiggs' () and 'tifton 85' ( sp.), 4 additi ...201627482693
nitrous oxide emission related to ammonia-oxidizing bacteria and mitigation options from n fertilization in a tropical soil.nitrous oxide (n2o) from nitrogen fertilizers applied to sugarcane has high environmental impact on ethanol production. this study aimed to determine the main microbial processes responsible for the n2o emissions from soil fertilized with different n sources, to identify options to mitigate n2o emissions, and to determine the impacts of the n sources on the soil microbiome. in a field experiment, nitrogen was applied as calcium nitrate, urea, urea with dicyandiamide or 3,4 dimethylpyrazone phosp ...201627460335
characterization of sugarcane (saccharum spp.) leaf senescence: implications for biofuel production.second-generation ethanol (2g-bioethanol) uses lignocellulosic feedstocks for ethanol production. sugarcane is one among the most suitable crops for biofuel production. its juice is extracted for sugar production, while sugarcane bagasse, straw, and senescing leaves are considered industrial waste. senescence is the age-dependent deterioration of plant cells, ultimately leading to cell death and completion of the plant life cycle. because senescing leaves may also be used for biofuel production, ...201627453728
utilization of vinasses as soil amendment: consequences and perspectives.vinasses are a residual liquid generated after the production of beverages, such as mezcal and tequila, from agave (agave l.), sugarcane (saccharum officinarum l.) or sugar beet (beta vulgaris l.). these effluents have specific characteristics such as an acidic ph (from 3.9 to 5.1), a high chemical oxygen demand (50,000-95,000 mg l(-1)) and biological oxygen demand content (18,900-78,300 mg l(-1)), a high total solids content (79,000 and 37,500 mg l(-1)), high total volatile solids 79,000 and 82 ...201627441131
analysis of biomechanical parameters in colonic anastomosis.the use of measures in colonic anastomoses to prevent dehiscences is of great medical interest. sugarcane molasses, which has adequate tolerability and compatibility in vivo, has not yet been tested for this purpose.201727438033
rnaseq reveals hydrophobins that are involved in the adaptation of aspergillus nidulans to lignocellulose.sugarcane is one of the world's most profitable crops. waste steam-exploded sugarcane bagasse (seb) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels. in nature, fungi seldom exist as planktonic cells, similar to those found in the nutrient-rich environment created within an industrial fermenter. instead, fungi predominantly form biofilms that allow them to thrive in hostile environments.201627437031
deciphering the transcriptomic response of fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease.pokkah boeng, caused by fusarium verticillioides, is a serious disease in sugarcane industry. the disease severity is related to the sugarcane genotype as well as environmental considerations, such as nitrogen application. the impact of the nitrogen source (ammonium sulfate, urea, or sodium nitrate) on sugarcane pokkah boeng disease and its pathogen was investigated in planta and fungal growth and sporulation production was measured in vitro. the results showed that ammonium and nitrate were ben ...201627434999
ima genome-f 6: draft genome sequences of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica and penicillium freii daomc 242723.the genomes of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica, and penicillium freii daomc 242723 are presented in this genome announcement. these six genomes are from plant pathogens and otherwise economically important fungal species. the genome sizes range from 21 mb in the case of ceratocystiopsis minuta to 58 mb for the basidiomycete armillaria fuscipes. these genomes include the first reports of genomes for the genus endoconidio ...201627433447
lessons learned from the microbial ecology resulting from different inoculation strategies for biogas production from waste products of the bioethanol/sugar industry.during strategic planning of a biogas plant, the local availability of resources for start-up and operation should be taken into consideration for a cost-efficient process. because most bioethanol/sugar industries in brazil are located in remote areas, the use of fresh cattle manure from local farms could be a solution for the inoculation of the biogas process. this study investigated the diversity and dynamics of bacterial and archaeal communities and the performance of biogas reactors inoculat ...201627429647
secretome data from trichoderma reesei and aspergillus niger cultivated in submerged and sequential fermentation methods.the cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. the proteomic analysis data presented here provide critical information to compare proteins secreted by trichoderma reesei and aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. the proteins were organized according to the families described in cazy ...201627419196
characterization of three plant biomass-degrading microbial consortia by metagenomics- and metasecretomics-based approaches.the selection of microbes by enrichment on plant biomass has been proposed as an efficient way to develop new strategies for lignocellulose saccharification. here, we report an in-depth analysis of soil-derived microbial consortia that were trained to degrade once-used wheat straw (ws1-m), switchgrass (sg-m) and corn stover (cs-m) under aerobic and mesophilic conditions. molecular fingerprintings, bacterial 16s ribosomal rna (rrna) gene amplicon sequencing and metagenomic analyses showed that th ...201627418359
parameters of fermentation and rumen microbiota of nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets.the objective of this study was to evaluate the effect of a fresh sugarcane-based diet and different roughage-to-concentrate ratios (70:30, 60:40, 40:60 and 20:80) on the rumen microbiota associated with rumen fermentation parameters and the intake and apparent digestibility of nutrients in nellore steers. eight rumen-cannulated nellore steers (331 ± 8 kg bw) were distributed in a double 4 × 4 latin square design balanced for the control of the residual effect. the ruminal ph decreased (p < 0.01 ...201627415825
a new genus and two new species of soldier fly (diptera: stratiomyidae: chiromyzinae) from australia, one found infesting sugarcane in central queensland.metridius nov. gen. and types species m. robertsoni nov. sp. with winged males and apterous females is described from adults and larvae found infesting sugarcane stools from near mackay, central queensland. a second new species, m. mcalpinei nov. sp., based only on males from near sydney, new south wales is also described. notes on the biology of both species and an identification key to the genera of the subfamily chiromyzinae and to the species are also given.201627394475
assessment of feed resources, feeding practices and coping strategies to feed scarcity by smallholder urban dairy producers in jimma town, ethiopia.smallholder dairy production is increasingly becoming popular in jimma town. however, feed shortage is a major constraint to dairy production. the objectives of this study was to assess feed resources, feeding practices and farmers' perceived causes of feed shortage and coping strategies to feed scarcity in smallholder dairy producers in jimma town, oromia regional state, ethiopia. a total of 54 randomly selected dairy farmers were interviewed using a pre-tested structured questionnaire and thro ...201627375986
unlocking the bacterial and fungal communities assemblages of sugarcane microbiome.plant microbiome and its manipulation herald a new era for plant biotechnology with the potential to benefit sustainable crop production. however, studies evaluating the diversity, structure and impact of the microbiota in economic important crops are still rare. here we describe a comprehensive inventory of the structure and assemblage of the bacterial and fungal communities associated with sugarcane. our analysis identified 23,811 bacterial otus and an unexpected 11,727 fungal otus inhabiting ...201627358031
treatment of sugarcane vinasse by combination of coagulation/flocculation and fenton's oxidation.the efficiency of individual and integrated processes applied to organic matter reduction and biodegradability improvement of a biodigested sugarcane vinasse wastewater was assessed. strategies considered were fenton's oxidation (strategy 1), coagulation/flocculation (strategy 2) and the combination of both processes (coagulation/flocculation followed by fenton's reaction) - strategy 3. it was found that fenton's oxidation per se allowed reducing the organic matter, increasing the wastewater bio ...201627353374
applying functional metagenomics to search for novel lignocellulosic enzymes in a microbial consortium derived from a thermophilic composting phase of sugarcane bagasse and cow manure.environments where lignocellulosic biomass is naturally decomposed are sources for discovery of new hydrolytic enzymes that can reduce the high cost of enzymatic cocktails for second-generation ethanol production. metagenomic analysis was applied to discover genes coding carbohydrate-depleting enzymes from a microbial laboratory subculture using a mix of sugarcane bagasse and cow manure in the thermophilic composting phase. from a fosmid library, 182 clones had the ability to hydrolyse carbohydr ...201627350392
genome-wide association mapping of quantitative traits in a breeding population of sugarcane.molecular markers associated with relevant agronomic traits could significantly reduce the time and cost involved in developing new sugarcane varieties. previous sugarcane genome-wide association analyses (gwas) have found few molecular markers associated with relevant traits at plant-cane stage. the aim of this study was to establish an appropriate gwas to find molecular markers associated with yield related traits consistent across harvesting seasons in a breeding population. sugarcane clones ...201627342657
badnaviruses: the current global scenario.badnaviruses (family: caulimoviridae; genus: badnavirus) are non-enveloped bacilliform dna viruses with a monopartite genome containing about 7.2 to 9.2 kb of dsdna with three to seven open reading frames. they are transmitted by mealybugs and a few species by aphids in a semi-persistent manner. they are one of the most important plant virus groups and have emerged as serious pathogens affecting the cultivation of several horticultural crops in the tropics, especially banana, black pepper, cocoa ...201627338451
fusarium sacchari, a cause of mycotic keratitis among sugarcane farmers - a series of four cases from north india.the two most common filamentous fungi causing mycotic keratitis are aspergillus and fusarium spp. around 70 fusarium spp. are involved in causing human infections. in this study, four cases of keratitis in sugarcane farmers in india are being reported, caused by the sugar cane pathogen fusarium sacchari, a species of the fusarium fujikuroi species complex. fusarial keratitis was established by potassium hydroxide/calcofluor white wet mounts and fungal culture of corneal scrapings on conventional ...201627292696
design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: optimization and synergism studies.lignocellulosic materials represent a very important and promising source of renewable biomass. in order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of trichoderma harzianum ioc 3844, penicillium funiculosum atcc 11797, and aspergillus niger atcc 1004, result ...201627254751
biohydrogen production from hydrolysates of selected tropical biomass wastes with clostridium butyricum.biohydrogen production has received widespread attention from researchers in industry and academic fields. response surface methodology (rsm) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with clostridium butyrium, and achieved the highest production rate and yield of hydrogen. highest h2 yield of 2.02 mol h2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °c, while the composition of medium was (g/l): 15.66 glucose, 6.0 ...201627251222
effect of ph, temperature, and chemicals on the endoglucanases and β-glucosidases from the thermophilic fungus myceliophthora heterothallica f.2.1.4. obtained by solid-state and submerged cultivation.this work reports endoglucanase and beta-glucosidase production by the thermophilic fungus myceliophthora heterothallica in solid-state (ssc) and submerged (smc) cultivation. wheat bran and sugarcane bagasse were used for ssc and cardboard for smc. highest endoglucanase production in ssc occurred after 192 hours: 1,170.6 ± 0.8 u/g, and in smc after 168 hours: 2,642 ± 561 u/g. the endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their ...201627242927
gh10 xyna is the main xylanase identified in the crude enzymatic extract of paenibacillus sp. a59 when grown on xylan or lignocellulosic biomass.a novel bacterial isolate with polysaccharides degrading activity was identified as paenibacillus sp., and named paenibacillus sp. a59. even though it is a strict mesophile, optimal xylanase activity of the crude enzymatic extract was achieved between 50°c and 70°c and more than 60% of the activity was retained after incubation for 48h at 50°c, indicating thermotolerance of the enzymes involved. the extract was also active on pre-treated sugarcane residue (scr) and wheat straw, releasing xylobio ...201727242139
secretome analysis of trichoderma reesei and aspergillus niger cultivated by submerged and sequential fermentation processes: enzyme production for sugarcane bagasse hydrolysis.cellulases and hemicellulases from trichoderma reesei and aspergillus niger have been shown to be powerful enzymes for biomass conversion to sugars, but the production costs are still relatively high for commercial application. the choice of an effective microbial cultivation process employed for enzyme production is important, since it may affect titers and the profile of protein secretion. we used proteomic analysis to characterize the secretome of t. reesei and a. niger cultivated in submerge ...201627241292
characterization and biotechnological application of recombinant xylanases from aspergillus nidulans.two xylanases from aspergillus nidulans, xlnb and xlnc, were expressed in pichia pastoris, purified and characterized. xlnb and xlnc achieved maximal activities at 60°c and ph 7.5 and at 50°c and ph 6.0, respectively. xlnb showed to be very thermostable by maintaining 50% of its original activity after 49h incubated at 50°c. xlnb had its highest activity against wheat arabinoxylan while xlnc had the best activity against beechwood xylan. both enzymes were completely inhibited by sds and hgcl2. x ...201627235731
comparison between brazilian agro-wastes and activated carbon as adsorbents to remove ni(ii) from aqueous solutions.this research was performed to find an alternative, low-cost, competitive, locally available and efficient adsorbent to treat nickel (ni) containing effluents. for this purpose, several brazilian agro-wastes like sugarcane bagasse (scb), passion fruit wastes (pfw), orange peel (op) and pineapple peel (pp) were compared with an activated carbon (ac). the adsorbents were characterized. effects of fundamental factors affecting the adsorption were investigated using batch tests. kinetic and equilibr ...201627232408
exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification.glycoside hydrolases (ghs) and accessory proteins are key components for efficient and cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. currently, commercialized ghs and accessory proteins are produced by ascomycetes. however, the role of wood decay basidiomycetes proteins in biomass saccharification has not been extensively pursued. wood decay fungi degrade polysaccharides in highly lignified tissues in natural environments, and are a promisin ...201627222665
determination of pesticides in sugarcane juice employing microextraction by packed sorbent followed by gas chromatography and mass spectrometry.this paper describes the development of a method for the determination of six pesticides (tebuthiuron, carbofuran, atrazine, metribuzine, ametryn, and bifenthrin) in sugarcane juice using microextraction by packed sorbent as the extraction technique. the extraction steps were optimized by factorial design, being the variables ph, ionic strength, desorption solvent and solvent volume optimized for comparisons among sorbent materials. among the evaluated materials c18-chromabond(®) showed better e ...201627219489
co-cultivation of aspergillus nidulans recombinant strains produces an enzymatic cocktail as alternative to alkaline sugarcane bagasse pretreatment.plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. a clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. however, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. replacing these methods with co-culti ...201627199917
resistance of sugarcane cultivars to mahanarva fimbriolata (stål) (hemiptera: cercopidae).the spittlebug mahanarva fimbriolata (stål) (hemiptera: cercopidae) is one of the most important pests of the sugarcane crop in brazil. despite of its importance, there is currently a lack of information regarding sugarcane cultivars' resistance to the spittlebug. therefore, our objective was to evaluate the response of sugarcane genotypes to this species. two experiments were carried out under laboratory conditions using a random block design with treatments in a factorial arrangement of 2 × 13 ...201427193410
fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare.pressmud is a substrate derived from sugarcane juice filtrate, and around 26-40  kg of this residue are produced per ton of sugarcane. it is mainly used as fertilizer in crops, and its application in the field is often made without any prior treatment, but, in this research, it was studied for the risk this practice poses for human health. this research was stimulated by previous results indicating the presence of opportunistic pathogens in residues used in various composting systems and the ext ...201627170376
air pollutants and hospital admission due to pneumonia in children: a time series analysis.the aim of this study was to estimate the association between expo sure to air pollutants and hospitalization for pneumonia among children in a medium-sized city located in the sugar cane plantation region of são paulo state.201627167545
tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance.grasses are lignocellulosic materials useful to supply the billion-tons annual requirement for renewable resources that aim to produce transportation fuels and a variety of chemicals. however, the polysaccharides contained in grass cell walls are built in a recalcitrant composite. deconstruction of these cell walls is still a challenge for the energy-efficient and economically viable transformation of lignocellulosic materials. the varied tissue-specific distribution of cell wall components adds ...201627148403
effect of treating sugarcane bagasse with urea and calcium hydroxide on feed intake, digestibility, and rumen fermentation in beef cattle.four beef cattle with initial body weight of 283 ± 14 kg were randomly allocated according to a 4 × 4 latin square design to study on the effect of feeding sugarcane bagasse (sb) treated with urea and/or calcium hydroxide (ca(oh)2) on feed intake, digestibility, and rumen fermentation. the treatments were as follows: rice straw (rs), untreated sb (sb), 4 % urea-treated sb (sbu), and 2 % urea + 2 % ca(oh)2-treated sb (sbuc), respectively. the results revealed that cattle fed with sbu and sbuc had ...201627139254
loss of soil (macro)fauna due to the expansion of brazilian sugarcane acreage.land use changes (luc) from pasture to sugarcane (saccharum spp.) crop are expected to add 6.4mha of new sugarcane land by 2021 in the brazilian cerrado and atlantic forest biomes. we assessed the effects of these luc on the abundance and community structure of animals that inhabit soils belowground through a field survey using chronosequences of land uses comprising native vegetation, pasture, and sugarcane along a 1000-km-long transect across these two major tropical biomes in brazil. macrofau ...201627135579
effect of ozonolysis pretreatment parameters on the sugar release, ozone consumption and ethanol production from sugarcane bagasse.a l9(3)(4) orthogonal array (oa) experimental design was applied to study the four parameters considered most important in the ozonolysis pretreatment (moisture content, ozone concentration, ozone/oxygen flow and particle size) on ethanol production from sugarcane bagasse (scb). statistical analysis highlighted ozone concentration as the highest influence parameter on reaction time and sugars release after enzymatic hydrolysis. the increase on reaction time when decreasing the ozone/oxygen flow ...201627132222
reproductive isolation between two populations of diatraea saccharalis (f.) (lepidoptera: crambidae) from different host plant species and regions in argentina.the sugarcane borer, diatraea saccharalis (f.), has a widespread distribution throughout the western hemisphere and is a pest of many crop plants including sugarcane, corn, sorghum and rice. the use of bacillus thuringiensis (bt) corn has been the primary tool for managing this species in corn fields. sugarcane borer control has been recently threatened by observations of susceptibility and/or resistance to certain varieties of bt corn and the protein used in many newer varieties. this has led t ...201627112423
boosting tag accumulation with improved biodiesel production from novel oleaginous microalgae scenedesmus sp. iitrind2 utilizing waste sugarcane bagasse aqueous extract (sbae).this investigation utilized sugarcane bagasse aqueous extract (sbae), a nontoxic, cost-effective medium to boost triacylglycerol (tag) accumulation in novel fresh water microalgal isolate scenedesmus sp. iitrind2. maximum lipid productivity of 112 ± 5.2 mg/l/day was recorded in microalgae grown in sbae compared to modified bbm (26 ± 3 %). carotenoid to chlorophyll ratio was 12.5 ± 2 % higher than in photoautotrophic control, indicating an increase in photosystem ii activity, thereby increasing g ...201627093970
transgenic sugarcane with a cry1ac gene exhibited better phenotypic traits and enhanced resistance against sugarcane borer.we developed sugarcane plants with improved resistance to the sugarcane borer, diatraea saccharalis (f). an expression vector pgcry1ac0229, harboring the cry1ac gene and the selectable marker gene, bar, was constructed. this construct was introduced into the sugarcane cultivar fn15 by particle bombardment. transformed plantlets were identified after selection with phosphinothricin (ppt) and basta. plantlets were then screened by pcr based on the presence of cry1ac and 14 cry1ac positive plantlet ...201627093437
valorization of agro-industrial wastes towards the production of rhamnolipids.in this work, oil mill wastewater (omw), a residue generated during olive oil extraction, was evaluated as an inducer of rhamnolipid production. using a medium containing as sole ingredients corn steep liquor (10%, v/v), sugarcane molasses (10%, w/v) and omw (25%, v/v), pseudomonas aeruginosa #112 produced 4.5 and 5.1g of rhamnolipid per liter in flasks and reactor, respectively, with critical micelle concentrations as low as 13mg/l. furthermore, in the medium supplemented with omw, a higher pro ...201627092993
in situ enzyme aided adsorption of soluble xylan biopolymers onto cellulosic material.the functional properties of cellulose fibers can be modified by adsorption of xylan biopolymers. the adsorption is improved when the degree of biopolymers substitution with arabinose and 4-o-methyl-glucuronic acid (meglca) side groups, is reduced. α-l-arabinofuranosidase (abfb) and α-d-glucuronidase (agua) enzymes were applied for side group removal, to increase adsorption of xylan from sugarcane (saccharum officinarum l) bagasse (bh), bamboo (bambusa balcooa) (bm), pinus patula (pp) and eucaly ...201627083357
flint corn grain processing and citrus pulp level in finishing diets for feedlot cattle.four trials were conducted to evaluate the effects of flint corn processing and the replacement of corn with citrus pulp (cip) in diets for nellore feedlot cattle. in a 103-d finishing trial, 216 nellore bulls (350 ± 24 kg initial bw) were used in a randomized complete block design with a 2 × 4 factorial arrangement of treatments. factors included 2 processing methods, either ground corn (gc) or steam-flaked corn (fc), with cip replacing each corn type at 4 levels (0, 25, 50, and 75% of dm). all ...201627065137
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