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amylolytic enzymes acquired from l-lactic acid producing enterococcus faecium k-1 and improvement of direct lactic acid production from cassava starch.an amylolytic lactic acid bacterium isolate k-1 was isolated from the wastewater of a cassava starch manufacturing factory and identified as entercoccus faecium based on 16s rrna gene sequence analysis. an extracellular α-amylase was purified to homogeneity and the molecular weight of the purified enzyme was approximately 112 kda with optimal ph value and temperature measured of 7.0 and 40 °c, respectively. it was stable at a ph range of 6.0-7.0, but was markedly sensitive to high temperatures a ...201728236189
the industrial applications of cassava: current status, opportunities and prospects.cassava (manihot esculenta crantz) is a drought-tolerant, staple food crop that is grown in tropical and subtropical areas. as an important raw material, cassava is a valuable food source in developing countries and is also extensively employed for producing starch, bioethanol and other bio-based products (e.g. feed, medicine, cosmetics and biopolymers). these cassava-based industries also generate large quantities of wastes/residues rich in organic matter and suspended solids, providing great p ...201728233322
preparation and characterization of bionanocomposite film based on tapioca starch/bovine gelatin/nanorod zinc oxide.to exploring a nano-packaging materials for using as coating or edible films, tapioca starch/gelatin/nanorod zno (znon) bionanocomposites were prepared via solution casting technique. the effects of nanofiller addition on the mechanical, physicochemical, and crystalline structures, as well as the barrier properties of bionanocomposite films were investigated. x-ray diffraction analysis showed that the bionanocomposite film incorporated with znon at a concentration of 3.5% w/w exhibited high inte ...201728232108
fundamental study on the impact of gluten-free starches on the quality of gluten-free model breads.starch is widely used as an ingredient and significantly contributes to texture, appearance, and overall acceptability of cereal based foods, playing an important role due to its ability to form a matrix, entrapping air bubbles. a detailed characterisation of five gluten-free starches (corn, wheat, rice, tapioca, potato) was performed in this study. in addition, the influence of these starches, with different compositional and morphological properties, was evaluated on a simple gluten-free model ...201628231125
novel process combining anaerobic-aerobic digestion and ion exchange resin for full recycling of cassava stillage in ethanol fermentation.a novel cleaner ethanol production process has been developed. thin stillage is treated initially by anaerobic digestion followed by aerobic digestion and then further treated by chloride anion exchange resin. this allows the fully-digested and resin-treated stillage to be completely recycled for use as process water in the next ethanol fermentation batch, which eliminates wastewater discharges and minimizes consumption of fresh water. the method was evaluated at the laboratory scale. process pa ...201728223080
molecular assessment of the bacterial community associated with cassava (manihot esculenta crantz) cultivation in cameroon.bacterial communities play an important role in nutrient cycles and plant development. their distribution and activity may depend on location and environmental heterogeneity. this study characterized soil bacterial communities in cassava fields of eastern (andom) and southern (bityili) cameroon using molecular tools. in both sites, two improved varieties (tms-96/1414; tms-92/0326) and a local variety (local) were grown in a randomized block design. composite bulk soils were collected at 10months ...201728219522
the role of type iii effectors from xanthomonas axonopodis pv. manihotis in virulence and suppression of plant immunity.xanthomonas axonopodis pv. manihotis (xam) causes cassava bacterial blight, the most important bacterial disease of cassava. xam, like other xanthomonas species, requires type iii effectors (t3es) for maximal virulence. xam strain cio151 possesses 17 predicted t3es belonging to the xanthomonas outer protein (xop) class. this work aimed to characterize nine xop effectors present in xam cio151 for their role in virulence and modulation of plant immunity. our findings demonstrate the importance of ...201728218447
case of escape in cassava, manihot esculenta crantz.two cassava escapes where collected from cultivated fields near natural habitat in bolivia. they are described morphologically and analyzed cytogenetically in this study. it is suggested that they are the product of backcrosses of cassava interspecific hybrids with the cultigen itself, and that selective conditions have developed in which certain forms of cassava segregates have adapted to grow wildly in natural habitats near cassava fields. these segregates may hybridize with cultivated cassava ...201728198512
tapioca cardiomyopathy: curse of cassava endomyocardial fibrosis.tropical endomyocardial fibrosis is a rare entity in the present era. restrictive cardiomyopathy due to tapioca consumption is very rare, although it has been reported in india, especially in state of kerala. we report a rare case of restrictive cardiomyopathy secondary to tapioca consumption in a 20-year-old male patient.201528197237
bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides.several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ...201728197131
the trade in african medicinal plants in matonge-ixelles, brussels (belgium).maintaining cultural identity and preference to treat cultural bound ailments with herbal medicine are motivations for migrants to continue using medicinal plants from their home country after moving to europe and the usa. as it is generally easier to import exotic food than herbal medicine, migrants often shift to using species that double as food and medicine. this paper focuses on the trade in african medicinal plants in a congolese neighborhood in brussels (belgium). what african medicinal p ...201628179733
expression of a mutated spt15 gene in saccharomyces cerevisiae enhances both cell growth and ethanol production in microaerobic batch, fed-batch, and simultaneous saccharification and fermentations.the spt15 gene encodes a saccharomyces cerevisiae tata-binding protein, which is able to globally control the transcription levels of various metabolic and regulatory genes. in this study, a spt15 gene mutant (s42n, s78r, s163p, and i212n) was expressed in s. cerevisiae by4741 (bspt15-m3), of which effects on fermentative yeast properties were evaluated in a series of culture types. by applying different nitrogen sources and air supply conditions in batch culture, organic nitrogen sources and mi ...201728168313
a virus-derived stacked rnai construct confers robust resistance to cassava brown streak disease.cassava brown streak disease (cbsd) threatens food and economic security for smallholder farmers throughout east and central africa, and poses a threat to cassava production in west africa. cbsd is caused by two whitefly-transmitted virus species: cassava brown streak virus (cbsv) and ugandan cassava brown streak virus (ucbsv) (genus: ipomovirus, family potyviridae). although varying levels of tolerance have been achieved through conventional breeding, to date, effective resistance to cbsd withi ...201628149300
explaining the texture properties of whey protein isolate/starch co-gels from fracture structures.the effects of tapioca starch (ts) and potato starch (ps) on texture properties of whey protein isolate (wpi)/starch co-gels were investigated for fracture structures. we focused on two types of wpi network structures. in a fine-stranded structure at ph 6.8, the wpi/ts co-gel fractured similarly to the wpi single gel. the wpi/ps co-gel was broken at a lower strain and lower stress. in a random aggregation at ph 5.8, the wpi/ts co-gel reached a yielding point at a lower strain, whereas the wpi/ps ...201728140770
field level rnai-mediated resistance to cassava brown streak disease across multiple cropping cycles and diverse east african agro-ecological locations.cassava brown streak disease (cbsd) presents a serious threat to cassava production in east and central africa. currently, no cultivars with high levels of resistance to cbsd are available to farmers. transgenic rnai technology was employed to combat cbsd by fusing coat protein (cp) sequences from ugandan cassava brown streak virus (ucbsv) and cassava brown streak virus (cbsv) to create an inverted repeat construct (p5001) driven by the constitutive cassava vein mosaic virus promoter. twenty-fiv ...201628127301
epigenetic map and genetic map basis of complex traits in cassava population.cassava (manihot esculenta crantz) is an important tropical starchy root crop that is adapted to drought but extremely cold sensitive. a cold-tolerant, high-quality, and robust supply of cassava is urgently needed. here, we clarify genome-wide distribution and classification of ccgg hemi-methylation and full-methylation, and detected 77 much candidate qtlsepi for cold stress and 103 much candidate qtlsepi for storage root quality and yield in 186 cassava population, generated by crossing two non ...201728120898
cellulase with high β-glucosidase activity by penicillium oxalicum under solid state fermentation and its use in hydrolysis of cassava residue.in this study, we investigated cellulase production by penicillium oxalicum eu2106 under solid-state fermentation (ssf) and its hydrolysis efficiency toward naoh-h2o2-pretreated cassava residue (nhcr) produced after bioethanol fermentation. optimization of ssf cultivation conditions for p. oxalicum eu2106 using a box-behnken design-based response-surface methodology resulted in maximal cellulase activity of 34.0 ± 2.8 filter-paper units/g dry substrate, exhibiting a ~ twofold increase relative t ...201728120306
the histone deacetylase inhibitor suberoylanilide hydroxamic acid alleviates salinity stress in cassava.cassava (manihot esculenta crantz) demand has been rising because of its various applications. high salinity stress is a major environmental factor that interferes with normal plant growth and limits crop productivity. as well as genetic engineering to enhance stress tolerance, the use of small molecules is considered as an alternative methodology to modify plants with desired traits. the effectiveness of histone deacetylase (hdac) inhibitors for increasing tolerance to salinity stress has recen ...201628119717
gene expression atlas for the food security crop cassava.cassava (manihot esculenta) feeds c. 800 million people world-wide. although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration and the roots contain low nutritional content. here, we provide molecular identities for 11 cassava tissue/organ types through rna-sequencing and develop an open access, web-based interface for further interrogation of the data. through this dataset, we consider the physiology of cassava. ...201728116755
characterisation, in vitro digestibility and expected glycemic index of commercial starches as uncooked ingredients.in this study native starches as ingredients (corn, rice, wheat, tapioca and potato) were characterized for microstructure, physicochemical, functional and thermal properties, in vitro digestibility and glycemic index. there was a significant variation in the granule shape and size distribution of the starches. particle size monomodal (corn, tapioca, potato) and bimodal (rice, wheat) distribution was observed amongst the starches. the potato starch showed the biggest size granules while the rice ...201628115752
some nutritional characteristics of enzymatically resistant maltodextrin from cassava (manihot esculenta crantz) starch.cassava (manihot esculenta crantz) native starch was treated with pyroconversion and enzymatic hydrolysis to produce a pyrodextrin and an enzyme-resistant maltodextrin. some nutritional characteristics were quantified for both compounds. pyroconversion was done using a 160:1 (p/v) starch:hcl ratio, 90 °c temperature and 3 h reaction time. the resulting pyrodextrin contained 46.21% indigestible starch and 78.86% dietary fiber. thermostable α-amylase (0.01%) was used to hydrolyze the pyrodextrin a ...201728102511
the calcium-dependent protein kinase (cdpk) and cdpk-related kinase gene families in hevea brasiliensis-comparison with five other plant species in structure, evolution, and expression.calcium-dependent protein kinases (cdpks or cpks) play important roles in various physiological processes of plants, including growth and development, stress responses and hormone signaling. although the cdpk gene family has been characterized in several model plants, little is known about this gene family in hevea brasiliensis (the para rubber tree). here, we characterize the entire h. brasiliensis cdpk and cdpk-related kinase (crk) gene families comprising 30 cdpk genes (hbcpk1 to 30) and nine ...201728097084
waste-to-biofuel: production of biobutanol from sago waste residues.the main concern of extensive production of biobutanol has been associated with the high cost of the substrate and the relatively low tolerance of clostridia to biobutanol production. in this study, the use of fermentable cassava waste residue (cwr) as substrate for biobutanol production was investigated using solvent-tolerant clostridium sp. four of obligatory, solvent-producing bacteria were isolated from sago industry waste sites. the nsw, pnas1, sb5 and sbi4 strains showed identical profiles ...201728091177
genome sequence of phytomonas françai, a cassava (manihot esculenta) latex parasite.here, we report the genome sequence of the cassava (manihot esculenta) latex parasite phytomonas françai p. françai infection is linked with the yield-loss disease "chochamento de raizes" (empty roots) in the unha variety of cassava, a disease characterized by poor root development and chlorosis of the leaves.201728082482
yeast mixture of liquid beer and cassava pulp with rice straw for the growth of dairy heifers.this study was conducted to determine the effects of mixtures of liquid brewer's yeast (lby) and cassava pulp (cvp) with rice straw (rs) on feed intake, digestibility, rumen fermentation, and growth of dairy heifers. sixteen holstein crossbred heifers (13.8 ± 1.6 months old, 210 ± 23 kg body weight (bw)) were randomly allocated to four feeding treatments with four replications, which were 0:0:100 (rs), 0:70:30 (0%lby), 20:50:30 (20%lby), and 50:20:30 (50%lby), respectively, for lby/cvp/rs on a f ...201728078531
tapioca maltodextrin in the production of soft unripened cheese.an excessive consumption of fat has been associated with an increased risk of health problems such as obesity, diabetes and cardiovascular diseases. cheese is a highly concentrated product which is rich in protein and minerals such as calcium and phosphorus and essential amino acids, therefore it is an important food in the diet. but low fat cheeses are usually characterized as having poor body and flavour. therefore,  it is crucial to find ways of improving the acceptability of the product. the ...201728071038
deinbollia mosaic virus: a novel begomovirus infecting the sapindaceous weed deinbollia borbonica in kenya and tanzania.four isolates of a bipartite begomovirus from naturally infected deinbollia borbonica plants exhibiting yellow mosaic symptoms in kenya and tanzania were molecularly characterised. the dna-a was most closely related to that of tomato leaf curl mayotte virus (am701764; 82%), while the dna-b shared the highest nucleotide sequence identity with that of east african cassava mosaic virus (aj704953) at 65%. based on the current ictv species demarcation criterion for the genus begomovirus (≥91% sequenc ...201728070648
studies on the production of alkaline α-amylase from bacillus subtilis cb-18.amylases are among the main enzymes used in food and other industries. they hydrolyse starch molecules into polymers composing glucose units. amylases have potential applications in a number of industrial processes including foods and pharmaceutical industries. alkaline α-amylase has the potential of hydrolysing starch under alkaline ph and is useful in the starch and textile industries and as an ingredient of detergents. amylases are produced from plants, however, microbial production processes ...201728068022
selecting native arbuscular mycorrhizal fungi to promote cassava growth and increase yield under field conditions.the use of arbuscular mycorrhizal fungal (amf) inoculation in sustainable agriculture is now widespread worldwide. although the use of inoculants consisting of native amf is highly recommended as an alternative to commercial ones, there is no strategy to allow the selection of efficient fungal species from natural communities. the objective of this study was (i) to select efficient native amf species (ii) evaluate their impact on nematode and water stresses, and (iii) evaluate their impact on ca ...201628066381
overproduction of superoxide dismutase and catalase confers cassava resistance to tetranychus cinnabarinus.to explore the role of protective enzymes in cassava (manihot esculenta crantz) resistance to mites, transgenic cassava lines overproducing copper/zinc superoxide dismutase (mecu/znsod) and catalase (mecat1) were used to evaluate and molecularly confirm cassava resistance to tetranychus cinnabarinus. laboratory evaluation demonstrated that, compared with the control cultivar tms60444 (wild type, wt), the survival, reproduction, development and activities of sod and cat in t. cinnabarinus feeding ...201728054665
attraction of coffee bean weevil, araecerus fasciculatus, to volatiles from the industrial yeast kluyveromyces lactis.the coffee bean weevil (cbw), araecerus fasciculatus (de geer, 1775) (coleoptera: anthribidae) is an important pest of stored products such as grains, coffee beans, cassava, and traditional chinese medicine materials. in china, cbw causes large losses of daqu, a traditional chinese liquor fermentation starter, and, unfortunately, the use of conventional insecticides against cbw is not suitable in daqu storage. we found cbw to be highly attracted to fermenting yeast cultures, such as kluyveromyce ...201728032269
a socio-scientific analysis of the environmental and health benefits as well as potential risks of cassava production and consumption.due to its high adaptability, cassava (manihot esculenta crantz) is one of the world's most cultivated and consumed plants after maize and rice. however, there are relatively few scientific studies on this important crop. the objective of this review was therefore to summarize and discuss the available information on cassava cropping in order to promote sustainable practices in terms of production and consumption. cassava cultivation has been expanding recently at the global scale and is widely ...201728013470
isolation and characterization of cellulose nanofibers from cassava root bagasse and peelings.this work aimed to obtain and characterize nanofibers from cassava bagasse and peelings, which are waste originating from cassava starch extraction. to isolate the nanofibers, a combination of pre-treatments (alkaline treatment, q-chelating treatment, bleaching), acid hydrolysis, and a final ultrasonic disintegration step were used. acidic hydrolysis was conducted at a constant temperature of 60°c; the acid concentration (30, 40, and 50%) and the treatment time (30, 60, and 90min) were varied. t ...201727988015
thermomechanical characterization of an amylose-free starch extracted from cassava (manihot esculenta, crantz).the aim of this study was to determine and compare the melting (tm), glass transition (tg) and mechanical relaxation (tα) temperatures of a new waxy cassava starch. thermal transitions measurements were obtained by differential scanning calorimetry (dsc) and dynamical mechanical thermal analysis (dmta). the experimental data showed a high correlation between water volume fraction and melting temperature (tm) indicating that the flory-huggins theory can be used to describe the thermal behavior of ...201727987895
β-glucans and resistant starch alter the fermentation of recalcitrant fibers in growing pigs.interactions among dietary ingredients are often assumed non-existent when evaluating the nutritive value and health effects of dietary fiber. specific fibers can distinctly affect digestive processes; therefore, digestibility and fermentability of the complete diet may depend on fiber types present. this study aimed to evaluate the effects of readily fermentable fibers (β-glucans and resistant starch) on the degradation of feed ingredients containing more persistent, recalcitrant, fibers. six s ...201627911928
identification and expression analyses of new potential regulators of xylem development and cambium activity in cassava (manihot esculenta).we have identified new potential regulators of xylem cell-type determination and cellular proliferation in cassava and studied their expression in roots. results are highly relevant for cassava biotechnology. cassava's root system is composed of two types of root that coexist in every individual: the fibrous and the storage roots. whether a root becomes fibrous or storage depends on the xylem cell types that it develops: fibrous roots develop xylem fibres and vessels while storage roots develop ...201727900471
the comparatively proteomic analysis in response to cold stress in cassava plantlets.cassava (manihot esculenta crantz) is a tropical root crop and sensitive to low temperature. however, it is poorly to know how cassava can modify its metabolism and growth to adapt to cold stress. an investigation aimed at a better understanding of cold-tolerant mechanism of cassava plantlets was carried out with the approaches of physiology and proteomics in the present study. the principal component analysis of seven physiological characteristics showed that electrolyte leakage (el), chlorophy ...201627881899
the inhibition effect of starch nanoparticles on tyrosinase activity and its mechanism.the objective of the current research was to investigate the effects of starch nanoparticles (snps) prepared from waxy maize, potato, normal corn, and tapioca starches on the activity of tyrosinase. as a main polyphenol oxidase, tyrosinase not only induces fruit and vegetable browning but also causes skin diseases by overproducing melanin. herein, for the first time, we evaluated the inhibitory kinetics of snps on tyrosinase. it turned out that snps inhibited tyrosinase activity reversibly. the ...201627872930
potential of tannin-rich plants, leucaena leucocephala, glyricidia sepium and manihot esculenta, to reduce enteric methane emissions in sheep.an in vivo trial was conducted in sheep to investigate the effect of three tropical tannin-rich plants (trp) on methane emission, intake and digestibility. the trp used were leaves of glyricidia sepium, leucaena leucocephala and manihot esculenta that contained, respectively, 39, 75 and 92 g condensed tannins/kg dm. methane was determined with the sulphur hexafluoride tracer technique. eight rumen-cannulated sheep of two breeds (four texel, four blackbelly) were used in two 4 × 4 latin square de ...201627870287
the erf transcription factor family in cassava: genome-wide characterization and expression analyses against drought stress.cassava (manihot esculenta) shows strong tolerance to drought stress; however, the mechanisms underlying this tolerance are poorly understood. ethylene response factor (erf) family genes play a crucial role in plants responding to abiotic stress. currently, less information is known regarding the erf family in cassava. herein, 147 erf genes were characterized from cassava based on the complete genome data, which was further supported by phylogenetic relationship, gene structure, and conserved mo ...201627869212
effect of acetic acid in recycling water on ethanol production for cassava in an integrated ethanol-methane fermentation process.recently, the integrated ethanol-methane fermentation process has been studied to prevent wastewater pollution. however, when the anaerobic digestion reaction runs poorly, acetic acid will accumulate in the recycling water. in this paper, we studied the effect of low concentration of acetic acid (≤25 mm) on ethanol fermentation at different initial ph values (4.2, 5.2 or 6.2). at an initial ph of 4.2, ethanol yields increased by 3.0% and glycerol yields decreased by 33.6% as the acetic acid conc ...201627858795
determination and prediction of digestible and metabolizable energy concentrations in byproduct feed ingredients fed to growing pigs.an experiment was conducted to determine digestible energy (de) and metabolizable energy (me) of different byproduct feed ingredients fed to growing pigs, and to generate prediction equations for the de and me in feed ingredients.201727857027
effect of genotype and genotype by environment interaction on total cyanide content, fresh root, and starch yield in farmer-preferred cassava landraces in tanzania.high starch yield is the most important trait for commercialized cassava starch production. furthermore, cyanide present in cassava roots poses a health challenge in the use of cassava for food. cassava genotypes have varying maturity periods that are also environmental dependent. this study aimed at identifying suitable cultivars and optimum time of harvest to maximize starch production across three environments. the study found significant difference between genotypes, locations, harvest perio ...201627826428
the potential of using biotechnology to improve cassava: a review.the importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. plant biotechnology offers a wide range of opportunities that can help cassava become a better crop for a constantly changing world. we therefore review the state of knowledge on the current use of biotechnology applied to cassava cultivars and its implications for breeding the crop into the ...201627818605
new accuracy estimators for genomic selection with application in a cassava (manihot esculenta) breeding program.genomic selection is the main force driving applied breeding programs and accuracy is the main measure for evaluating its efficiency. the traditional estimator (te) of experimental accuracy is not fully adequate. this study proposes and evaluates the performance and efficiency of two new accuracy estimators, called regularized estimator (re) and hybrid estimator (he), which were applied to a practical cassava breeding program and also to simulated data. the simulation study considered two indivi ...201627808382
in vitro assessment of the genotoxic and cytotoxic effects of boiled juice (tucupi) from manihot esculenta crantz roots.the population of pará (a state in brazil) has a very characteristic food culture, as a majority of the carbohydrates consumed are obtained from cassava (manihot esculenta crantz) derivatives. tucupi is the boiled juice of cassava roots that plays a major role in the culinary footprint of pará. before boiling, this juice is known as manipueira and contains linamarin, a toxic glycoside that can decompose to hydrogen cyanide. in this study, the cytotoxic and genotoxic effects of tucupi on cultured ...201627808379
cassava brown streak virus has a rapidly evolving genome: implications for virus speciation, variability, diagnosis and host resistance.cassava is a major staple food for about 800 million people in the tropics and sub-tropical regions of the world. production of cassava is significantly hampered by cassava brown streak disease (cbsd), caused by cassava brown streak virus (cbsv) and ugandan cassava brown streak virus (ucbsv). the disease is suppressing cassava yields in eastern africa at an alarming rate. previous studies have documented that cbsv is more devastating than ucbsv because it more readily infects both susceptible an ...201627808114
diallel analysis of provitamin a carotenoid and dry matter content in cassava (manihot esculenta crantz).global efforts are underway to biofortify cassava (manihot esculenta crantz) with provitamin a carotenoids to help combat dietary vitamin a deficiency afflicting the health of more than 500 million resource-poor people in sub-saharan africa. to further the biofortification initiative in uganda, a 6×6 diallel analysis was conducted to estimate combining ability of six provitamin a clones and gene actions controlling total carotenoid content (tcc), dry matter content (dmc) in cassava roots and oth ...201627795688
eleven years of breeding efforts to combat cassava brown streak disease.cassava (manihot esculenta crantz) production is currently under threat from cassava brown streak disease (cbsd), a disease that is among the seven most serious obstacles to world's food security. three issues are of significance for cbsd. firstly, the virus associated with cbsd, has co-evolved with cassava outside its center of origin for at least 90 years. secondly, that for the last 74 years, cbsd was only limited to the low lands. thirdly, that most research has largely focused on cbsd epide ...201627795681
rooting for cassava: insights into photosynthesis and associated physiology as a route to improve yield potential.contents 50 i. 50 ii. 52 iii. 54 iv. 55 v. 57 vi. 57 vii. 59 60 references 61 summary: as a consequence of an increase in world population, food demand is expected to grow by up to 110% in the next 30-35 yr. the population of sub-saharan africa is projected to increase by > 120%. in this region, cassava (manihot esculenta) is the second most important source of calories and contributes c. 30% of the daily calorie requirements per person. despite its importance, the average yield of cassava in af ...201727778353
efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch-digesting glucoamylase from penicillium oxalicum.starch is a very abundant and renewable carbohydrate and is an important feedstock for industrial applications. the conventional starch liquefaction and saccharification processes are energy-intensive, complicated, and not environmentally friendly. raw starch-digesting glucoamylases are capable of directly hydrolyzing raw starch to glucose at low temperatures, which significantly simplifies processing and reduces the cost of producing starch-based products.201627777618
virtual water and water self-sufficiency in agricultural and livestock products in brazil.virtual water trade is often considered a solution for restricted water availability in many regions of the world. brazil is the world leader in the production and export of various agricultural and livestock products. the country is either a strong net importer or a strong net exporter of these products. the objective of this study is to determine the volume of virtual water contained in agricultural and livestock products imported/exported by brazil from 1997 to 2012, and to define the water s ...201627760721
tropical soil fertility changes under monocultures and successional communities of different structure.for 5 yr we monitored the fertility of a volcanic-ash derived inceptisol at a site in the humid tropics of costa rica. after forest felling and burning, we established four treatments in a randomized block design with six blocks: a sequence of monocultures (two crops of maize [zea mays] followed by cassava [manihot esculenta], then the tree species cordia alliodora), successional vegetation, a mimic of successional vegetation that was physiognomically similar to the model but shared no species w ...199127755770
comprehensive transcriptional and functional analyses of melatonin synthesis genes in cassava reveal their novel role in hypersensitive-like cell death.melatonin is a widely known hormone in animals. since melatonin was discovered in plants, more and more studies highlight its involvement in a wide range of physiological processes including plant development and stress responses. many advances have been made in the terms of melatonin-mediated abiotic stress resistance and innate immunity in plants, focusing on model plants such as rice and arabidopsis. in this study, 7 melatonin synthesis genes were systematically analyzed in cassava. quantitat ...201627739451
genetic diversity in cassava landraces grown on farms in alta floresta-mt, brazil.brazil is considered one of the domestication centers of cassava (manihot esculenta), containing a large part of the biological diversity and traditional knowledge of the species. the aim of the present study was to evaluate the genetic diversity of cassava landraces grown by farmers in the north of mato grosso state, brazil, using inter simple sequence repeat (issr) molecular markers. the study was carried out in the municipality of alta floresta, mt, on farms located in two rural areas. sevent ...201627706696
differential response of cassava genotypes to infection by cassava mosaic geminiviruses.mitigation of cassava mosaic disease (cmd) focuses on the introgression of resistance imparted by the polygenic recessive (cmd1), dominant monogenic (cmd2) and cmd3 loci. the mechanism(s) of resistance they impart, however, remain unknown. two cmd susceptible and nine cmd resistant cassava genotypes were inoculated by microparticle bombardment with infectious clones of african cassava mosaic virus cameroon strain (acmv-cm) and the kenyan strain k201 of east african cassava mosaic virus (eacmv ke ...201727693919
emergence of a latent indian cassava mosaic virus from cassava which recovered from infection by a non-persistent sri lankan cassava mosaic virus.the major threat for cassava cultivation on the indian subcontinent is cassava mosaic disease (cmd) caused by cassava mosaic geminiviruses which are bipartite begomoviruses with dna a and dna b components. indian cassava mosaic virus (icmv) and sri lankan cassava mosaic virus (slcmv) cause cmd in india. two isolates of slcmv infected the cassava cultivar sengutchi in the fields near malappuram and thiruvananthapuram cities of kerala state, india. the malappuram isolate was persistent when mainta ...201627690084
analysis of the complete genome sequence of euphorbia ringspot virus, an atypical member of the genus potyvirus.the complete genome sequence of an isolate of euphorbia ringspot virus (eursv) was determined by deep sequencing and rapid amplification of cdna ends (race) rt-pcr. it has an rna genome of 10,154 nucleotides in size, excluding the poly(a) tail, and encodes a polyprotein of 3265 amino acids. phylogenetic analysis from this study supports the earlier taxonomic assignment to the genus potyvirus; however, a gene encoding the ham1h protein, inserted between nib and cp of the eursv genome, which was p ...201727686070
new lupan-type triterpenoids.three new lupan-type triterpernoid derivatives, namely globimetulin a (1), b (2) and c (3), were isolated from the shoot of globimetula dinklagei (loranthaceae), a hemiparasitic plant growing on manihot esculenta, along with five known compounds: friedelin (4), friedelan-3-ol (5), 28-hydroxyfriedelin (6), 4-hydroxy-3,5-dimethoxybenzoic acid (7) and (1r,5s,7s)-7-[2-(4-hydroxyphenyl)ethyl]-2,6-dioxabicyclo[3.3.1]nonan-3-one (8). the structures of the new compounds were elucidated by detailed analy ...201627626765
effects of graded levels of liquid brewer's yeast on chemical composition and fermentation quality in cassava pulp and rice straw-based total mixed ration silage.this study aimed to evaluate the effect of liquid brewer's yeast (lby) addition on chemical composition and fermentation quality of mixture of lby and cassava pulp (cvp) with rice straw (rs) in different ratios during preservation periods. four mixtures of lby, cvp and rs were made, that is mixture ratio of lby : cvp : rs of 0% lby, 20% lby, 35% lby and 50% lby were 0:70:30, 20:50:30, 35:35:30 and 50:20:30 as fresh matter, respectively. the bags were opened at weeks 0, 1, 2, 4 and 8 after storag ...201727611881
divergent evolutionary and epidemiological dynamics of cassava mosaic geminiviruses in madagascar.cassava mosaic disease (cmd) in madagascar is caused by a complex of at least six african cassava mosaic geminivirus (cmg) species. this provides a rare opportunity for a comparative study of the evolutionary and epidemiological dynamics of distinct pathogenic crop-infecting viral species that coexist within the same environment. the genetic and spatial structure of cmg populations in madagascar was studied and bayesian phylogeographic modelling was applied to infer the origins of madagascan cmg ...201627600545
marker-based estimates reveal significant non-additive effects in clonally propagated cassava (manihot esculenta): implications for the prediction of total genetic value and the selection of varieties.in clonally propagated crops, non-additive genetic effects can be effectively exploited by the identification of superior genetic individuals as varieties. cassava (manihot esculenta crantz) is a clonally propagated staple food crop that feeds hundreds of millions. we quantified the amount and nature of non-additive genetic variation for three key traits in a breeding population of cassava from sub-saharan africa using additive and non-additive genome-wide marker-based relationship matrices. we ...201627587297
small rna and methylation responses in susceptible and tolerant landraces of cassava infected with south african cassava mosaic virus.endogenous small rnas (srnas) associated with gene regulatory mechanisms respond to virus infection, and virus-derived small rnas (vsrnas) have been implicated in recovery or symptom remission in some geminivirus-host interactions. transcriptional gene silencing (tgs) (24 nt vsrnas) and post transcriptional gene silencing (ptgs) (21-23 nt vsrnas) have been associated with geminivirus intergenic (ir) and coding regions, respectively. in this illumina deep sequencing study, we compared for the fir ...201627586073
translation, modification and cellular distribution of two ac4 variants of african cassava mosaic virus in yeast and their pathogenic potential in plants.plant infecting geminiviruses encode a small (a)c4 protein within the open reading frame of the replication-initiator protein. in african cassava mosaic virus, two in-frame start codons may be used for the translation of a longer and a shorter ac4 variant. both were fused to green fluorescent protein or glutathione-s-transferase genes and expressed in fission yeast. the longer variant accumulated in discrete spots in the cytoplasm, whereas the shorter variant localized to the plasma membrane. a ...201627584591
genome-wide identification of cassava r2r3 myb family genes related to abscission zone separation after environmental-stress-induced abscission.cassava plants (manihot esculenta crantz) resist environmental stresses by shedding leaves in leaf pulvinus abscission zones (azs), thus leading to adaptation to new environmental conditions. little is known about the roles of cassava r2r3 myb factors in regulating az separation. herein, 166 cassava r2r3 myb genes were identified. evolutionary analysis indicated that the 166 r2r3 myb genes could be divided into 11 subfamilies. transcriptome analysis indicated that 26 r2r3 myb genes were expresse ...201627573926
evaluating imputation algorithms for low-depth genotyping-by-sequencing (gbs) data.well-powered genomic studies require genome-wide marker coverage across many individuals. for non-model species with few genomic resources, high-throughput sequencing (hts) methods, such as genotyping-by-sequencing (gbs), offer an inexpensive alternative to array-based genotyping. although affordable, datasets derived from hts methods suffer from sequencing error, alignment errors, and missing data, all of which introduce noise and uncertainty to variant discovery and genotype calling. under suc ...201627537694
molecular cloning, subcellular localization and characterization of two adenylate kinases from cassava, manihot esculenta crantz cv. ku50.adenylate kinase (adk) is a phosphotransferase that plays an important role in cellular energy homeostasis. many isozymes located in different subcellular compartments have been reported. in this study, we focus on the characterization of cassava (manihot esculenta) adks. we found 15 adks that are publicly available in the african cassava genome database. we cloned two adks, namely meadk1 and meadk2, which are phylogenetically grouped together with the plastidial adk in potato. both meadk1 and m ...201627518222
resilience of cassava (manihot esculenta crantz) to salinity: implications for food security in low-lying regions.rising sea levels are threatening agricultural production in coastal regions due to inundation and contamination of groundwater. the development of more salt-tolerant crops is essential. cassava is an important staple, particularly among poor subsistence farmers. its tolerance to drought and elevated temperatures make it highly suitable for meeting global food demands in the face of climate change, but its ability to tolerate salt is unknown. cassava stores nitrogen in the form of cyanogenic glu ...201627506218
cassava traits and end-user preference: relating traits to consumer liking, sensory perception, and genetics.breeding efforts have focused on improving agronomic traits of the cassava plant however little research has been done to enhance the crop palatability. this review investigates the links between cassava traits and end-user preference in relation with sensory characteristics. the main trait is starch and its composition related to the textural properties of the food. pectin degradation during cooking resulted in increased mealiness. nutritional components such as carotenoids made the cassava yel ...201627494196
exposure to various abscission-promoting treatments suggests substantial erf subfamily transcription factors involvement in the regulation of cassava leaf abscission.cassava plants (manihot esculenta crantz) have obvious abscission zone (az) structures in their leaf pulvinus-petioles. cassava leaf abscission can be triggered by either 17 days of water-deficit stress or 4 days of ethylene treatment. to date, little is known about cassava ap2/erf factors, and less is known regarding their roles in regulating abscission zone development.201627488048
edible films based on cassava starch and fructooligosaccharides produced by bacillus subtilis natto cct 7712.the objectives of this work were to produce fructooligosaccharides (foss) by using the microorganism bacillus subtilis natto cct 7712 and to employ these foss as a functional ingredient in cassava starch edible films, which were characterized according to their microstructure, mechanical and barrier properties. the produced foss could be easily dissolved, resulting in homogeneous filmogenic solutions, which were easily manipulated to obtain films by casting. foss were added in different concentr ...201627474664
produce from africa's gardens: potential for leafy vegetable and fruit fermentations.a rich variety of indigenous fruits and vegetables grow in africa, which contribute to the nutrition and health of africa's populations. fruits and vegetables have high moisture and are thus inherently prone to accelerated spoilage. food fermentation still plays a major role in combating food spoilage and foodborne diseases that are prevalent in many of africa's resource disadvantaged regions. lactic acid fermentation is probably the oldest and best-accepted food processing method among the afri ...201627458430
quantitative, image-based phenotyping methods provide insight into spatial and temporal dimensions of plant disease.plant disease symptoms exhibit complex spatial and temporal patterns that are challenging to quantify. image-based phenotyping approaches enable multidimensional characterization of host-microbe interactions and are well suited to capture spatial and temporal data that are key to understanding disease progression. we applied image-based methods to investigate cassava bacterial blight, which is caused by the pathogen xanthomonas axonopodis pv. manihotis (xam). we generated xam strains in which in ...201627443602
characterization of a new cucurbit-infecting ipomovirus from sudan.two members of the genus ipomovirus (family potyviridae) are known to infect cucurbits: cucumber vein yellowing virus (cvyv), which is emerging throughout the mediterranean basin, and squash vein yellowing virus (sqvyv), which has been described in america and the caribbean basin, and more recently in israel. in this work, an ipomovirus different from cvyv and sqvyv, tentatively named coccinia mottle virus (cocmov), was detected in a sample of the cucurbit coccinia grandis collected in central s ...201627430562
interspecies and intraspecies analysis of trehalose contents and the biosynthesis pathway gene family reveals crucial roles of trehalose in osmotic-stress tolerance in cassava.trehalose is a nonreducing α,α-1,1-disaccharide in a wide range of organisms, and has diverse biological functions that range from serving as an energy source to acting as a protective/signal sugar. however, significant amounts of trehalose have rarely been detected in higher plants, and the function of trehalose in the drought-tolerant crop cassava (manihot esculenta crantz) is unclear. we measured soluble sugar concentrations of nine plant species with differing levels of drought tolerance and ...201627420056
application of edible coating with starch and carvacrol in minimally processed pumpkin.the present study evaluated the effect of an edible coating of cassava starch and carvacrol in minimally processed pumpkin (mpp). the minimal inhibitory concentration (mic) of carvacrol against escherichia coli, salmonella enterica serotype typhimurium, aeromonas hydrophila, and staphylococcus aureus was determined. the edible coating that contained carvacrol at the mic and 2 × mic was applied to mpp, and effects were evaluated with regard to the survival of experimentally inoculated bacteria an ...201627413224
deciphering the biology of deltasatellites from the new world: maintenance by new world begomoviruses and whitefly transmission.deltasatellites are small noncoding dna satellites associated with begomoviruses. the study presented here has investigated the biology of two deltasatellites found in wild malvaceous plants in the new world (nw). infectious clones of two nw deltasatellites (from malvastrum coromandelianum and sidastrum micranthum) and associated begomoviruses were constructed. infectivity in nicotiana benthamiana and their natural malvaceous hosts was assessed. the nw deltasatellites were not able to spread aut ...201627400152
properties of african cassava mosaic virus capsid protein expressed in fission yeast.the capsid proteins (cps) of geminiviruses combine multiple functions for packaging the single-stranded viral genome, insect transmission and shuttling between the nucleus and the cytoplasm. african cassava mosaic virus (acmv) cp was expressed in fission yeast, and purified by sds gel electrophoresis. after tryptic digestion of this protein, mass spectrometry covered 85% of the amino acid sequence and detected three n-terminal phosphorylation sites (threonine 12, serines 25 and 62). differential ...201627399762
application of amylomaltase for the synthesis of salicin-α-glucosides as efficient anticoagulant and anti-inflammatory agents.the focus of this study was the synthesis of α-glucosyl derivatives of salicin by a transglucosylation reaction. the reaction was catalyzed by recombinant amylomaltase using tapioca starch as a glucosyl donor. several reaction parameters, such as the enzyme-substrate concentrations, ph, temperature and incubation time, were optimized. using the optimum conditions, at least three products with retention times (rt) of 6.2, 9.2 and 14.1 were observed. the maximum yield of glucosylated salicin deriv ...201627394039
metabolite profiling of dioscorea (yam) species reveals underutilised biodiversity and renewable sources for high-value compounds.yams (dioscorea spp.) are a multispecies crop with production in over 50 countries generating ~50 mt of edible tubers annually. the long-term storage potential of these tubers is vital for food security in developing countries. furthermore, many species are important sources of pharmaceutical precursors. despite these attributes as staple food crops and sources of high-value chemicals, dioscorea spp. remain largely neglected in comparison to other staple tuber crops of tropical agricultural syst ...201627385275
changing dietary habits among akwen xerente.the research aimed to identify the current feed of xerente people in indian villages porteira and funil in the city of tocantinia / to. it was used a qualitative ethnographic approach. conducted from september 2013 to august 2014. the data were presented in a diary, through participant observation of food practices with the guidance of a script. a process of dietary change that permeates the culture of this people is taking place. factors such as the deficit in the planting gardens, the recent a ...201627384282
effects of different ratios and storage periods of liquid brewer's yeast mixed with cassava pulp on chemical composition, fermentation quality and in vitro ruminal fermentation.this study aims to evaluate the chemical composition, fermentation quality and in vitro ruminal fermentation of various ratios and storage periods of liquid brewer's yeast (lby) mixed with cassava pulp (cvp).201727383807
amazonian foods and implications for human biology.diets of subsistence-based amazonian populations have been linked to local resources, but are changing with market penetration.201627337942
control of linepithema micans (hymenoptera: formicidae) and eurhizococcus brasiliensis (hemiptera: margarodidae) in vineyards using toxic baits.linepithema micans (forel) (hymenoptera: formicidae) is the main ant species responsible for dispersal of eurhizococcus brasiliensis (wille) (hemiptera: margarodidae), a root scale that damages grapevines in southern brazil. the effects of different formulations of toxic baits based on boric acid and hydramethylnon to control l. micans and e. brasiliensis were evaluated. toxic baits with boric acid (1.0%) mixed in different concentrations of inverted sugar (20%, 30%, and 40%), and hydramethylnon ...201627329621
current knowledge and future research perspectives on cassava (manihot esculenta crantz) chemical defenses: an agroecological view.cassava (manihot esculenta crantz) is one of the most important staple crops worldwide. it constitutes the major source of carbohydrates for millions of low-income people living in rural areas, as well as a cash crop for smallholders in tropical and sub-tropical regions. the food and agriculture organization of the united nations predicts that cassava plantations will increase and production systems will intensify in the future, highlighting the need for developing strategies that improve the su ...201627316676
supramolecular structure of jackfruit seed starch and its relationship with digestibility and physicochemical properties.the influence of supramolecular structure on the physicochemical properties and digestibility of jackfruit seed starch (jss) were investigated. compared with maize and cassava starches (ms and cs), jss had smaller granules and higher amylose content (jss: 24.90%; cs: 16.68%; and ms: 22.42%), which contributed to higher gelatinization temperature (to: 81.11°c) and setback viscosity (548.9mpas). from scanning electron microscopy, the digestion of jss was observed mainly at the granule surface. due ...201627312638
performances and haematological profile of broilers fed fermented dried cassava (manihot esculenta crantz).the effect of feeding fermented dried cassava (gathot) on the performances and haematological profile of broilers was investigated. there were four dietary treatments arranged in a completely randomized design, i.e. control diet and diets containing 25, 50 or 100 g/kg gathot. the birds were provided with the treatment diets ad libitum from 8 to 35 days of age. body weight, feed intake and feed conversion ratio (fcr) were determined weekly. at day 32, the birds were blood sampled, sacrificed and ...201627307279
natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (manihot esculenta crantz) storage root.cassava (manihot esculenta crantz) storage root provides a staple food source for millions of people worldwide. increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-y ...201627286876
analysis of octenylsuccinate rice and tapioca starches: distribution of octenylsuccinic anhydride groups in starch granules.characterization of the fine structure of octenylsuccinic anhydride (osa) starch would lead to a better understanding of functional properties. osa rice and tapioca starches were analyzed using microscopy, liquid chromatography and nuclear magnetic resonance. chain length distribution of amylopectin changed significantly (p<0.05) after osa esterification. weight averaged degree of polymerization (dpw) decreased significantly (p<0.05) from 16.47 to 13.29 and from 14.87 to 12.47 in native and osa ...201627283674
interactive effects of temperature and drought on cassava growth and toxicity: implications for food security?cassava is an important dietary component for over 1 billion people, and its ability to yield under drought has led to it being promoted as an important crop for food security under climate change. despite its known photosynthetic plasticity in response to temperature, little is known about how temperature affects plant toxicity or about interactions between temperature and drought, which is important because cassava tissues contain high levels of toxic cyanogenic glucosides, a major health and ...201627252148
mineral and phytate contents of some prepared popular ghanaian foods.prepared ghanaian traditional foods, mostly consist of starchy staples such as yams (dioscorea spp.), cassava (manihot esculenta), millet (pennisetum glaucum), maize (zea mays) and rice (oryza sativa) etc. these traditional foods are a main source of energy and macronutrients. little or no information however exist on the mineral and phytate contents of prepared traditional ghanaian foods. the mineral and phytate contents of twenty commonly eaten ghanaian foods, prepared using popular recipes we ...201627247878
toward better understanding of postharvest deterioration: biochemical changes in stored cassava (manihot esculenta crantz) roots.food losses can occur during production, postharvest, and processing stages in the supply chain. with the onset of worldwide food shortages, interest in reducing postharvest losses in cassava has been increasing. in this research, the main goal was to evaluate biochemical changes and identify the metabolites involved in the deterioration of cassava roots. we found that high levels of ascorbic acid (asa), polyphenol oxidase (ppo), dry matter, and proteins are correlated with overall lower rates o ...201627247771
ethanol production from a biomass mixture of furfural residues with green liquor-peroxide saccarified cassava liquid.as the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production. cassava residues (cr) are byproducts of the cassava starch industry which can be mixed with lignocellulosic materials for ethanol production. the presence of lignin in lignocellulosic substrates can inhibit saccharification by reducing the cellulase activity. simultaneous saccharification and fermentation (ssf) of furfural residues (fr) pretreated with gree ...201627245838
validation of reference genes for relative quantitative gene expression studies in cassava (manihot esculenta crantz) by using quantitative real-time pcr.reverse transcription quantitative real-time polymerase chain reaction (real-time pcr, also referred to as quantitative rt-pcr or rt-qpcr) is a highly sensitive and high-throughput method used to study gene expression. despite the numerous advantages of rt-qpcr, its accuracy is strongly influenced by the stability of internal reference genes used for normalizations. to date, few studies on the identification of reference genes have been performed on cassava (manihot esculenta crantz). therefore, ...201627242878
biological implications in cassava for the production of amylose-free starch: impact on root yield and related traits.cassava (manihot esculenta, crantz) is an important food security crop, but it is becoming an important raw material for different industrial applications. cassava is the second most important source of starch worldwide. novel starch properties are of interest to the starch industry, and one them is the recently identified amylose-free (waxy) cassava starch. waxy mutants have been found in different crops and have been often associated with a yield penalty. there are ongoing efforts to develop c ...201627242813
[a rare cause of optic neuropathy: cassava].cassava root is a staple food for almost 500 million people worldwide. excessive consumption of it is a rare cause of optic neuropathy.201627236340
modified dietary fiber from cassava pulp and assessment of mercury bioaccessibility and intestinal uptake using an in vitro digestion/caco-2 model system.the ability of modified dietary fiber (mdf) generated from cassava pulp to modulate the bioaccessibility and intestinal absorption of heavy metals may be helpful to mitigate health risk associated with select foods including select fish high in methyl mercury. using a coupled in vitro digestion/caco-2 human intestinal cell model, the reduction of fish mercury bioaccessibility and intestinal uptake by mdf was investiaged. mdf was prepared from cassava pulp, a byproduct of tapioca production. the ...201627220052
production of cyclodextrin glycosyltransferase by immobilized bacillus sp. on chitosan matrix.the whole-cell immobilization on chitosan matrix was evaluated. bacillus sp., as producer of cgtase, was grown in solid-state and batch cultivation using three types of starches (cassava, potato and cornstarch). biomass growth and substrate consumption were assessed by flow cytometry and modified phenol-sulfuric acid assays, respectively. qualitative analysis of cgtase production was determined by colorless area formation on solid culture containing phenolphthalein. scanning electron microscopy ...201627194141
effect of cassava pulp supplement on 1,3-propanediol production by clostridium butyricum.to improve its 1,3-propanediol (1,3-pd) production, clostridium butyricum was cultivated on glycerol medium supplemented with cassava pulp (cp). at small concentrations, the cp improved the 1,3-pd productivity of c. butyricum from (0.25±0.01)g/l/h (glycerol alone) to (0.43±0.02)g/l/h (glycerol+2g/l cp) after 24h fermentation.201627184427
water desorption of cassava starch granules: a study based on thermogravimetric analysis of aqueous suspensions and humid powders.this work reports on water desorption from cassava starch in relation with the structure and conditioning of granules in suspensions or after equilibration in desiccators. the experimental work is performed by thermogravimetric analysis with isothermal and non-isothermal protocols and interpreted to derive the activation energies and desorption frequencies according to the humidity range with no adjustable parameter. the analysis points out the different types of water interacting with the starc ...201627178961
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