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tissue culture and top-fruit tree species.the commercial cultivation of rosaceous fruit trees (e.g., pear, apple, cherry, peach, plum) relies heavily upon the quality and performance of the rootstocks. this is even more the case now that self-rooted scions produce larger trees with a longer juvenile phase (1). it would, therefore, be of special interest for the fruit breeder to have general purpose rootstocks with a wide ecophysiological adaptation and high compatibility coupled with early cropping. in addition, many of the older and hi ...199021390607
change in sagittal balance with placement of an interspinous spacer.study design.: a prospective case series. objective.: to determine the effect of x-stop implantation on sagittal spinal balance using 36-inch films. summary of background data.: interspinous process spacers have been shown as an effective treatment of neurogenic claudication. the devices block the last few degrees of extension at the stenotic level, thus preventing compression of the nerve roots. these devices have been criticized because they may push the patient's spine into a kyphotic positio ...201121358476
pre-harvest application of putrescine influences japanese plum fruit ripening and quality.to investigate the role of pre-harvest application of putrescine (put) in regulating fruit ripening and quality of early-, mid- and late-season maturing japanese plum fruit, whole trees were sprayed with an aqueous solution containing put (0, 0.1, 1.0 and 2.0 mm) one week before anticipated commercial harvest for each cultivar separately. the put (2.0 mm) treatment reduced the respiration rate and ethylene production more than untreated fruit during fruit ripening at ambient temperature (20 ± 1 ...201021339121
frozen fruit skin prick test for the diagnosis of fruit allergy.diagnosis of fruit sensitisation by skin prick test (spt) is fast and easy to perform. nevertheless, some fruit is not available throughout the year. freezing aliquots of these fresh fruits to be defrosted would be a good solution to perform spt at any time.201021337912
interaction of plum pox virus with specific colloidal gold-labeled antibodies and development of immunochromatographic assay of the virus.two monoclonal antibodies (mabs) raised against plum pox virus (ppv) were shown to recognize its d, m, and c strains. conjugates of the antibodies with colloidal gold (cg) nanoparticles averaging 26 nm in diameter were synthesized. the binding constants of ppv with both the native and conjugated mabs were determined using a biacore x device. the complexes between the cg-mab conjugates and plum pox virions were examined by means of transmission electron and atomic force microscopy. using the conj ...201021314608
an analysis of the 1978 african swine fever outbreak in brazil and its eradication.the aim of this paper is to evaluate the african swine fever (asf) outbreak that began in 1978 in paracambi municipality, rio de janeiro state, brazil, and the steps taken by the brazilian authorities to eradicate the disease. the presence of asf in the country was confirmed by isolating the virus, and its pathogenicity was certified by the laboratory of the plum island disease center, new york, united states. even before the laboratory results became available, the brazilian agriculture ministr ...201021309454
physiological responses and differential gene expression in prunus rootstocks under iron deficiency conditions.two prunus rootstocks, the myrobalan plum p 2175 and the interspecific peach-almond hybrid, felinem, were studied to characterize their biochemical and molecular responses induced under iron-deficient conditions. plants of both genotypes were submitted to different treatments using a hydroponic system that permitted removal of fe from the nutrient solution. control plants were grown in 90 µm fe (iii)-edta, deficient plants were grown in an iron free solution, and plants submitted to an inductor ...201121306783
mediterranean and central-eastern european countries host viruses of two different clades of plum pox virus strain m.the genetic diversity of plum pox virus strain m (ppv-m) was assessed by analyzing 28 isolates collected in 8 european countries. two genomic fragments spanning the (cter)p3-6k1-(nter)ci coding region as well as the full coat protein coding region were sequenced directly from pcr products. phylogenetic analysis showed that the geographical origin of the collected isolates was clearly associated with two different ppv-m clades. moreover, the pattern of substitutions in the cp gene shed light on t ...201121293968
molecular variability analyses of apple chlorotic leaf spot virus capsid protein.the complete sequences of the coat protein (cp) gene of 26 isolates of apple chlorotic leaf spot virus (aclsv) from india were determined. the isolates were obtained from various pome (apple, pear and quince) and stone (plum, peach, apricot, almond and wild himalayan cherry) fruit trees. other previously characterized aclsv isolates and trichoviruses were used for comparative analysis. indian aclsv isolates among themselves and with isolates from elsewhere in the world shared 91-100% and 70-98% ...201021289443
field evaluation of reduced insecticide spray programs for managing plum curculio, conotrachelus nenuphar (coleoptera: curculionidae), in alabama peaches.plum curculio (pc), conotrachelus nenuphar (herbst.), is an important pest of peaches in the southeastern united states. commercially acceptable control of this insect is typically achieved by weekly or biweekly application of broad-spectrum conventional insecticides, resulting in 6-12 sprays per season. experiments were conducted in a peach orchard in alabama during 2007-2009 to compare the conventional calendar-based insecticide spray program involving weekly applications of phosmet with three ...201121268230
molecular, ultrastructural, and biological characterization of pennsylvania isolates of plum pox virus.plum pox virus (ppv) was identified in pennsylvania in 1999. the outbreak was limited to a four-county region in southern pennsylvania. initial serological and molecular characterization indicated that the isolates in pennsylvania belong to the d strain of ppv. the pennsylvania isolates were characterized by sequence analysis, electron microscopy, host range, and vector transmission to determine how these isolates related to their previously studied european counterparts. genetically, pennsylvan ...201121261466
ectopic expression of class 1 knox genes induce adventitious shoot regeneration and alter growth and development of tobacco (nicotiana tabacum l) and european plum (prunus domestica l).transgenic plants of tobacco (nicotiana tabacum l) and european plum (prunus domestica l) were produced by transforming with the apple class 1 knox genes (mdkn1 and mdkn2) or corn knox1 gene. transgenic tobacco plants were regenerated in vitro from transformed leaf discs cultured in a medium lacking cytokinin. ectopic expression of knox genes retarded shoot growth by suppressing elongation of internodes in transgenic tobacco plants. expression of each of the three knox1 genes induced malformatio ...201121212958
molecular epidemiology of plum pox virus in japan.for a molecular epidemiological study based on complete genome sequences, 37 plum pox virus (ppv) isolates were collected from the kanto region in japan. pair-wise analyses revealed that all 37 japanese isolates belong to the ppv-d strain, with low genetic diversity (less than 0.8%). in phylogenetic analysis of the ppv-d strain based on complete nucleotide sequences, the relationships of the ppv-d strain were reconstructed with high resolution: at the global level, the american, canadian, and ja ...201121198358
simple and efficient biolistic procedure for the plant transfection with cdna clones of rna viruses.simple, fast, low-cost, and efficient procedure for dna delivery to the cell nuclei of whole plants was developed. the procedure was optimized for the plum pox virus (ppv) and its host nicotiana benthamiana. it is based on the leaf bombardment with tungsten microparticles with bound dna using common air gun. the procedure did not require special equipment and source of driving gas. the transfection efficiency obtained by the newly developed procedure was close to 100%, but this level dropped wit ...201021175255
analysis of the epitope structure of plum pox virus coat protein.typing of the particular plum pox virus (ppv) strain responsible in an outbreak has important practical implications and is frequently performed using strain-specific monoclonal antibodies (mabs). analysis in western blots of the reactivity of 24 mabs to a 112-amino-acid n-terminal fragment of the ppv coat protein (cp) expressed in escherichia coli showed that 21 of the 24 mabs recognized linear or denaturation-insensitive epitopes. a series of eight c-truncated cp fragments allowed the mapping ...201121171886
diversity in destinations, routes and timing of small adult and sub-adult striped bass morone saxatilis on their southward autumn migration.almost three-quarters of the 46 young adult and sub-adult striped bass morone saxatilis that were acoustically tagged in plum island estuary, massachusetts, u.s.a., in the summer of 2006 were detected in one or more southern coastal arrays during their autumn migration. on the basis of the trajectories along which these m. saxatilis moved from feeding to overwintering areas, three migratory groups emerged. after leaving plum island estuary, about half of the fish were detected only in a mid-lati ...201021155786
effectiveness of different solid-phase microextraction fibres for differentiation of selected madeira island fruits based on their volatile metabolite profile--identification of novel compounds.a headspace solid-phase microextraction (hs-spme) procedure based on five commercialised fibres (85 μm polyacrylate - pa, 100 μm polydimethylsiloxane - pdms, 65 μm polydimethylsiloxane/divinylbenzene - pdms/dvb, 70 μm carbowax/divinylbenzene - cw/dvb and 85 μm carboxen/polydimethylsiloxane - car/pdms) is presented for the characterization of the volatile metabolite profile of four selected madeira island fruit species, lemon (citrus limon), kiwi (actinidia deliciosa), papaya (carica papaya l.) a ...201021147335
inactivation of salmonella spp. on tomatoes by plant molecules.the efficacy of carvacrol (car), trans-cinnamaldehyde (tc), eugenol (eug) and β-resorcylic acid (br) as a wash treatment for reducing salmonella spp. on tomatoes was investigated. plum tomatoes inoculated with a six-serotype mixture of salmonella (10⁸cfu) were subjected to washing in sterile deionized water (control) or deionized water containing chlorine (100 ppm), car (0.25 and 0.75%), tc (0.5 and 0.75%), eug (0.25 and 0.75%), or br (0.75 and 1.0%) for 15 sec, 1 min, and 3 min. the plant molec ...201021122935
harmonic radar: efficacy at detecting and recovering insects on agricultural host plants.in pest management research, harmonic radar systems have been largely used to study insect movement across open or vegetation-poor areas because the microwave signal is attenuated by the high water content of vegetation. this study evaluated whether the efficacy of this technology is sufficient to track insects in vegetative landscapes.201121058275
silencing of plum pox virus 5'utr/p1 sequence confers resistance to a wide range of ppv strains.an effective disease-control strategy should protect the host from the major economically important and geographically widespread variants of a pathogen. plum pox virus (ppv) is the causal agent of sharka, the most devastating viral disease of prunus species. we have shown previously that the hairpin rna expression driven by h-utr/p1, h-p1/hcpro, h-hcpro and h-hcpro/p3 constructs, derived from the ppv-m ispave44 isolate, confers resistance to the homologous virus in nicotiana benthamiana plants. ...201020963442
[observation on therapeutic effect of plum-blossom needle combined with medicated thread moxibustion of traditional zhuang nationality medicine on postherpetic neuralgia].to search for a better therapy for postherpetic neuralgia (phn) on the trunk.201020886801
report: prunes and liver function: a clinical trial.prunes are used by folks as a remedy of various diseases including hepatitis. a clinical trial was designed to see the effects of prunes (prunus domestica) on liver function. 166 healthy volunteers were divided into three groups randomly. either three (about 11.43g) or six (23g approx.) prunes were soaked in a glass of water (250ml) overnight. each subject from two test groups was asked to drink prune juice & eat whole fruit(single or double dose of prunes) as well, early in the morning, daily f ...201020884464
content of phenolic compounds and antioxidant capacity in fruits of apricot genotypes.research on natural compounds is increasingly focused on their effects on human health. in this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (prunus armeniaca l.) genotypes resistant against plum pox virus (ppv) cultivated on department of fruit growing of mendel university in brno. fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. antioxidant cap ...201020877223
plum curculio (coleoptera: curculionidae) adult mortality and associated fruit injury after exposure to field-aged insecticides on tart cherry branches.plum curculio, conotrachelus nenuphar (herbst) (coleoptera: curculionidae), adults were exposed to field-aged residues of thiamethoxam, acetamiprid, thiacloprid, indoxacarb, or azinphos-methyl on tart cherry, prunus cerasus l. variety montmorency. at 1, 3, 7, and 14 d postapplication, fruit were sampled for chemical residues, and bioassays were used to assess beetle mortality and plant tissue injury. azinphos-methyl had lethal activity within 1 d of exposure at all postapplication intervals and ...201020857728
[relationship between ige antibodies to recombinant allergens rbet v 1 and rbet v 2 and food causing oral allergy syndrome in cases of birch-pollen allergy].persons allergic to birch pollen often report oral and pharyngeal hypersensitivity to fruit and vegetables, such as apples and peaches due to immunological cross-reactivity, or oral allergy syndrome (oas) sometimes accompanied by systemic reaction. such cross-reactive antigen reactions involve bet v 1, the main birch-pollen allergen, and bet v 2, birch-pollen profilin. we evaluated the food/antigen relationship.201020845708
optimal timing of mr sialography by use of a simple method of stimulating the salivary gland: a preliminary report.the present study was performed for determining the optimal timing of mr sialography by use of the japanese pickled plum (umeboshi) for promoting secretion by the salivary glands. mr sialography was performed in four healthy male volunteers. the four volunteers were examined before and 10 min after stimulation with umeboshi. on the next examination, three volunteers were examined before and after umeboshi stimulation every 1 min up to 5 min to allow assessment of the temporal changes in duct vis ...200820821149
fred plum (1924-2010) and the founding of the annals of neurology. 201020818781
snack selection influences nutrient intake, triglycerides, and bowel habits of adult women: a pilot study.because appropriate snacking can promote a healthy body weight and serve as an important contributor to a healthy diet for women, identification of suitable foods for incorporation between meals is essential. we investigated the influence of short-term (2 weeks) incorporation of 100-kcal servings of snacks of dried plums vs low-fat cookies twice daily on total energy and nutrient intake, biochemical parameters, and bowel habits in a randomized crossover design of two 2-week trials separated by a ...201020800123
dietary dried plum increases bone mass in adult and aged male mice.bone is progressively lost with advancing age. therapies are limited and the only effective proanabolic regimen presently available to restore bone is intermittent treatment with teriparatide (parathyroid hormone 1-34). recent evidence suggests that dietary supplementation with dried plum (dp) can prevent bone loss due to estrogen deficiency. to determine whether dietary dp supplementation can prevent the loss of bone with aging and whether bone that has already been lost can be restored, adult ...201020739449
effects of using plum puree on some properties of low fat beef patties.the purpose of this study was to determine the effects of using different amounts of plum puree (5%, 10% or 15%) on some properties of low fat beef patties. plum puree (pp) was used as an extender in beef patties. moisture content decreased with increasing concentration of plum puree. increasing amounts of pp decreased beef patty ph. the highest cooking yield and moisture retention were found in 5% pp samples. diameter reduction increased and thickness reduction decreased with increasing amounts ...201020732751
wild plum: novel particles of improved optical brightness and fluorescence.a novel compound named wild plum fluoresces blue, and has been synthesized to camouflage skin imperfections, addressing the market demand for an anti-aging product. wild plum imparts optical brightness and fluorescence and can be used as an ingredient in cosmetic formulations. skin appearance before and after application of wild plum compounds demonstrated an improved appearance of skin including a decreased number of wrinkles. when added to makeup, lotions, creams, and powders, wild plum convey ...201020716436
fruits and vegetables protect against the genotoxicity of heterocyclic aromatic amines activated by human xenobiotic-metabolizing enzymes expressed in immortal mammalian cells.heterocyclic aromatic amines (haas) can be formed during the cooking of meat and fish at elevated temperatures and are associated with an increased risk for cancer. on the other hand, epidemiological findings suggest that foods rich in fruits and vegetables can protect against cancer. in the present study three teas, two wines, and the juices of 15 fruits and 11 vegetables were investigated for their protective effect against the genotoxic effects of 2-amino-3-methylimidazo[4,5-f]quinoline (iq) ...201020713174
cornelian cherry (cornus mas l.) juices as a source of minerals in human diet.the aim of this study was to determine the mineral content of cornelian cherry (cornus mas l.), as this fruit and its preservatives may be considered as important nutritional supplements. potassium (k), calcium (ca), sodium (na), iron (fe), zinc (zn), manganese (mn), and copper (cu) were present in cornelian cherry juice as measured by atomic absorption. compared to other juices obtained from plum, pear, and apple, cornelian cherry juice contained high levels of ca, reaching 10-fold higher (323 ...201020706938
o-glcnacylation of the plum pox virus capsid protein catalyzed by secret agent: characterization of o-glcnac sites by electron transfer dissociation mass spectrometry.the capsid protein of plum pox virus (ppv-cp) is modified with o-linked β-n-acetylglucosamine (o-glcnac). in arabidopsis thaliana this modification is made by an o-glcnac transferase named secret agent (sec). modification of ppv-cp by sec is hypothesized to have a direct role in the infection process, because virus titer and rate of spread are reduced in sec mutants. previous studies used deletion mapping and site-directed mutagenesis to identify four o-glcnac sites on the capsid protein that ar ...201120676902
coniochaeta (lecythophora), collophora gen. nov. and phaeomoniella species associated with wood necroses of prunus trees.species of the genus coniochaeta (anamorph: lecythophora) are known as pathogens of woody hosts, but can also cause opportunistic human infections. several fungi with conidial stages resembling lecythophora were isolated from necrotic wood samples of prunus trees in south africa. in order to reveal their phylogenetic relationships, these fungi were studied on a morphological and molecular (5.8s nrdna, its-1, its-2, gapdh, ef-1alpha, 28s nrdna, 18s nrdna) basis. some of the isolates were identifi ...201020664761
benzothiadiazole and l-2-oxothiazolidine-4-carboxylic acid reduce the severity of sharka symptoms in pea leaves: effect on antioxidative metabolism at the subcellular level.the effect of treatment with benzothiadiazole (bth) or l-2-oxothiazolidine-4-carboxylic acid (otc), and their interaction with plum pox virus (ppv) infection, on antioxidative metabolism of pea plants was studied at the subcellular level. ppv infection produced a 20% reduction in plant growth. pre-treatment of pea plants with otc or bth afforded partial protection against ppv infection, measured as the percentage of leaves showing symptoms, but neither bth nor otc significantly reduced the virus ...201020653891
plum-branch-like carbon nanofibers decorated with sno2 nanocrystals.novel plum-branch-like carbon nanofibers (cnfs) decorated with sno2 nanocrystals have been synthesized by electrospinning and subsequent thermal treatment in an ar/h2o atmosphere. the morphologies of the as-synthesized sno2/cnf composites and the contents of carbon and sno2 can be controlled by adjusting the heat treatment temperature. it is proposed that the growth of sno2/cnf composites follows the outward diffusion of tin composites from the as-spun tin composite/polyacrylonitrile (pan) nanof ...201020648300
o-antigen diversity and lateral transfer of the wbe region among vibrio splendidus isolates.the o-antigen is a highly diverse structure expressed on the outer surface of gram-negative bacteria. the products responsible for o-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. while heterogeneous o-antigens are observed within vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. here, we investigate o-antigen diversity among coastal marine vibrio splendidus-like isolates. the wbe region was first ...201020629700
analysis of expressed sequence tags from prunus mume flower and fruit and development of simple sequence repeat markers.expressed sequence tag (est) has been a cost-effective tool in molecular biology and represents an abundant valuable resource for genome annotation, gene expression, and comparative genomics in plants.201020626882
constraints to virus infection in nicotiana benthamiana plants transformed with a potyvirus amplicon.plant genomes have been transformed with full-length cdna copies of viral genomes, giving rise to what has been called 'amplicon' systems, trying to combine the genetic stability of transgenic plants with the elevated replication rate of plant viruses. however, amplicons' performance has been very variable regardless of the virus on which they are based. this has boosted further interest in understanding the underlying mechanisms that cause this behavior differences, and in developing strategies ...201020604920
fruit and vegetable consumption among older adults by tooth loss and socio-economic status.the aim of this study was to examine consumption of fruit and vegetables in relation to tooth loss and income.201020604755
polarographic assay based on hydrogen peroxide scavenging in determination of antioxidant activity of strong alcohol beverages.total antioxidant (ao) activity of strong alcohol beverages such as wine and plum brandies, whiskeys, herbal and sweet fruit liqueurs have been assessed using a polarographic assay based on hydrogen peroxide scavenging (hps). rank of order of total ao activity, expressed as percentage of decrease of anodic oxidation current of hydrogen peroxide, was found analogous with total phenolic content estimated by folin-ciocalteau (fc) assay and radical scavenging capacity against the stable free radical ...201020604507
active carbons prepared by chemical activation of plum stones and their application in removal of no2.a technology of obtaining active carbon from plum stones by chemical activation with koh is described. the effect of carbonisation temperature as well as activation procedure on the textural parameters, acid-base character of the surface and sorption properties of active carbons has been checked. the sorption properties of the activated carbons obtained were characterised by determination of nitrogen dioxide adsorption in dry and wet conditions. the final products were microporous activated carb ...201020576355
genotypic analysis of xylella fastidiosa isolates from different hosts using sequences homologous to the xanthomonas rpf genes.summary this is the first report of a genotypic analysis of the phytopathogenic bacteria xylella fastidiosa (xf) using differences within intra- and intergenic regions of pathogenic genes. orthologous sequences from the genome of xf were identified for genes involved in the regulation of pathogenicity factors (rpf) from xanthomonas campestris pv. campestris (xcc). while the rpf genes were conserved, the chromosomal region revealed differences in gene sizes and intergenic spacings and a major tra ...200320569393
field evaluation of traps and lures for monitoring plum curculio (coleoptera: curculionidae) in alabama peaches.the plum curculio, conotrachelus nenuphar (herbst) (coleoptera: curculionidae), is a key pest of peaches, prunus persica (l.) batsch, in north america. we evaluated the effectiveness of two widely used trap types (pyramid versus circle traps) and commercially available synthetic lures for monitoring the pest in two peach orchards in alabama during 2008 and 2009. the lures evaluated alone or in combinations included benzaldehyde (bz) (a component of fruit odor), plum essence (pe) (a mixture of fr ...201020568620
expression of a modified nucleocapsid-protein of tomato spotted wilt virus (tswv) confers resistance against tswv and groundnut ringspot virus (grsv) by blocking systemic spread.summary tobacco transformed with a construct comprising the nucleocapsid-protein (n) gene of tomato spotted wilt virus (tswv) and the 5' non-translated leader sequence of plum pox virus (ppv) as a translation enhancer displays unusually broad resistance against tospoviruses. contrary to expectations, the transgenic plants did not express a higher level of tswv n protein than control plants harbouring the n gene without the ppv enhancer. instead, expression of an n-terminally elongated n protein, ...200420565598
[clinical observation on plum-blossom needle on governor vessel and point jiaji (ex-b 2) for treatment of cerebral palsy of involuntary movement].to probe into an effective therapy for treatment of cerebral palsy of involuntary movement.201020518168
genetic variation among date plum (diospyros lotus) genotypes in turkey.turkey is one of the most important genetic resources of the date plum, diospyros lotus, especially in the northeastern part of the country. authenticating the identity of germplasm resources of d. lotus would be of great value for breeding. we examined the genetic variability of 11 d. lotus genotypes sampled from coruh valley in turkey. one hundred and twenty-eight dna markers were generated by 12 random primers. the highest polymorphism ratio was observed with the primer opa-01 (71%) while the ...201020506085
development and validation of a multiresidue method for the determination of neonicotinoid and macrocyclic lactone pesticide residues in milk, fruits, and vegetables by ultra-performance liquid chromatography/ms/ms.a multiresidue method was developed and validated for the determination of 13 neonicotinoid pesticides and metabolites, and nine macrocyclic lactone pesticides and veterinary drugs using spe and ultra-performance liquid chromatography/ms/ms. the method was validated in milk, orange, spinach, apple, plum, watermelon, green bean, zucchini, broccoli, strawberry, grape, and tomato by analyzing replicates of residue-free control samples fortified with a mixture of 22 target analytes at three concentr ...201020480883
novel phaeoacremonium species associated with necrotic wood of prunus trees.the genus phaeoacremonium is associated with opportunistic human infections, as well as stunted growth and die-back of various woody hosts, especially grapevines. in this study, phaeoacremonium species were isolated from necrotic woody tissue of prunus spp. (plum, peach, nectarine and apricot) from different stone fruit growing areas in south africa. morphological and cultural characteristics as well as dna sequence data (5.8s rdna, its1, its2, beta-tubulin, actin and 18s rdna) were used to iden ...200820467488
novel paraconiothyrium species on stone fruit trees and other woody hosts.coniothyrium-like fungi are common wood and soil inhabitants and hyperparasites on other fungi. they belong to different fungal genera within the pleosporales. several isolates were obtained on wood of different prunus species (plum, peach and nectarine) from south africa, on actinidia species from italy and on laurus nobilis from turkey. morphological and cultural characteristics as well as dna sequence data (5.8s nrdna, its1, its2, partial ssu nrdna) were used to characterise them. the isolate ...200820467483
adsorption of copper onto agriculture waste materials.mining activities generate large amounts of wastewater, which contain heavy metals of elevated concentration according to legislative threshold values. therefore, treatment is necessary, and there has been a recent focus on more environmentally friendly methods. one of these is biosorption, where heavy metals are adsorbed from the wastewater via materials of biological origin. in this work copper sorption capacity, kinetics and isotherms of different low-cost residual agricultural materials was ...201020462688
the need for culture collections to support plant pathogen diagnostic networks.plant-pathogenic microorganisms, by virtue of their size, similarity in disease symptoms and closely related morphologies, are notoriously difficult to diagnose and detect. diagnosis gives proof as to the causal agent of disease and is important for developing appropriate control measures. detection shows the presence of a microorganism and is of importance for safeguarding national and international trade. live reference collections are required to characterize the taxonomy and function of micr ...201020457251
identification and molecular characterization of a new potyvirus from panax notoginseng.a potyvirus causing distortion and mosaic symptoms in the herbal plant sanqi (panax notoginseng) was isolated from yunnan province, china, and the complete nucleotide sequence of one isolate and the partial sequences of two other isolates were determined. the viral rna genome comprised 9,750 nt excluding the 3'-terminal poly(a) tail, with the capacity to encode a single polyprotein of 3,089 amino acids. phylogenetic analysis with other completely sequenced potyviruses revealed that the virus in ...201020446003
a single amino acid mutation alters the capsid protein electrophoretic double-band phenotype of the plum pox virus strain ppv-rec.plum pox virus (ppv) isolates differ by their capsid protein (cp) mobility in sds-page. these electrophoretic phenotypes are likely to result from post-translational modifications of the cp. we demonstrated that the cp mobility was solely determined by the cp n-terminal region. sequence comparison pinpointed a possible role of mutations at position 66 in determining the cp phenotype of ppv-rec isolates. site-directed mutagenesis of a chimeric clone demonstrated that gly(66) in the cp resulted in ...201020422234
qualitative effects of fresh and dried plum ingredients on vacuum-packaged, sliced hams.boneless ham muscles (semimembranosus+adductor) were injected (20% w/w) with a curing brine containing no plum ingredient (control), fresh plum juice concentrate (fp), dried plum juice concentrate (dp), or spray dried plum powder (pp) at 2.5% or 5%. hams were cooked, vacuum-packaged, stored at<4 degrees c and evaluated at 2-week intervals over 10 week. evaluations were performed on sliced product to determine cook loss, vacuum-package purge, allo-kramer shear force, 2-thiobarbituric acid-reactiv ...200920416628
rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana.endogenous eukaryotic rna-dependent rna polymerases (rdrs) produce double-stranded rna intermediates in diverse processes of small rna synthesis in rna silencing pathways. rdr6 is required in plants for posttranscriptional gene silencing induced by sense transgenes (s-ptgs) and has an important role in amplification of antiviral silencing. whereas rdr1 is also involved in antiviral defense in plants, this does not necessarily proceed through triggering silencing. in this study, we show that nico ...201020400679
analysis of wolbachia strains associated with conotrachelus nenuphar (coleoptera: curculionidae) in the eastern united states.we studied the distribution patterns of wolbachia infection associated with plum curculio strains in eight states of the eastern united states. the presence of the wolbachia-specific gene wsp identified infections of this endosymbiont in 97.8% of the 93 samples tested. three distinct wolbachia strains were identified. the strains wcne1 (593 bp) and wcne2 (593 bp) were 97% identical, and their sequences were both 84% identical with wcne3 (590 bp). blastn searches through genbank showed strong sim ...201020388268
development and validation of an algorithm to establish a total antioxidant capacity database of the us diet.estimation of total antioxidant intake is the first step to investigate the protective effects of antioxidants on oxidative stress-mediated disease. the present study was designed to develop an algorithm to estimate total antioxidant capacity (tac) of the us diet. tac of individual antioxidants and 50 popular antioxidant-rich food items in the us diet were determined by 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (abts) assay and the 1,1-diphenyl-2-picrylhydrazyl (dpph) assay. theoreti ...201020377495
sensory, chemical, and electronic tongue assessment of micro-oxygenated wines and oak chip maceration: assessing the commonality of analytical techniques.micro-oxygenation (mox) was conducted in the presence and absence of oak chips at rates to mimic oxygen ingress during barrel maturation of red wine. following mox, wines were analyzed for chemical attributes pertaining to phenolic composition and assessed by a trained sensory panel. an electronic tongue (et) was also used to assess the wines. variations in chemical attributes were found to be mostly influenced by vintage, followed by oak chip maceration accounting for 48% and 16% of variation w ...201020356080
correlation between the composition of multivalent antibody conjugates with colloidal gold nanoparticles and their affinity.interactions between multivalent preparations of antibodies (conjugated with colloidal gold nanoparticles (gnp) as a carrier system) and a multivalent ligand were investigated. the aim of the present study was to reveal the relationship between the affinity of the conjugate and its composition (i.e., the valency). surface plasmon resonance was applied to study the affinity and the kinetics of the interaction of multivalent conjugates and multivalent virus (on the example of the plum pox virus (p ...201020347832
control of low-temperature injury in the victoria plum. 194720295245
benzene hexachloride for control of plum curculio on peaches. 194720264504
domain-growth kinetic origin of nonhorizontal phase coexistence plateaux in langmuir monolayers: compression rigidity of a raft-like lipid distribution.the present work addresses the question of a nonhorizontal coexistence plateau found in the liquid-expanded (le) to liquid-condensed (lc) transition of langmuir monolayers of lipid amphiphiles, which is apparently incongruent with the first-order character of this main le/lc phase transition. this pathology is understood in a mechanical context as a resistance of the monolayer against compression giving rise to a nonzero rigidity in the coexistence region. surface rheology has allowed for a quan ...201020235509
influence of indigenous yeasts on the fermentation and volatile profile of plum brandies.the aim of this study was to determine the influence of different yeasts isolated from fresh blue plum fruits (aureobasidium sp.) and spontaneously fermenting plum musts (kloeckera apiculata and saccharomyces cerevisiae), as well as commercial wine and distillery strains, on the fermentation and chemical composition of plum brandies. gas chromatography methods were used to detect major volatile components. the most rapid fermentation occurred in musts inoculated with s. cerevisiae. however, the ...201020227608
monitoring of the entomological diversity in a pesticide free orchard: investigation of the gembloux agricultural university conservatory.in orchards, the fruit production is currently oriented to integrated pest management. in order to develop efficient pest control strategy by using entomophagous beneficial and lowering the application of pesticides, sufficient knowledge of entomological populations is needed. here, a systematic monitoring of insect diversity and abundance in a conservatory orchard of gembloux was performed. combining yellow traps and visual observations, 19 pest and 34 beneficial families were identified among ...200920222593
harmonic radar: assessing the impact of tag weight on walking activity of colorado potato beetle, plum curculio, and western corn rootworm.the impact of electronic dipole tags on the walking activity of three insects was determined using video tracking software. results varied within and between the three species studied. the heaviest tag mounted on the pronotum of the colorado potato beetle, leptinotarsa decemlineata (say), reduced its mean horizontal speed slightly but significantly. the mean horizontal speed of plum curculio, conotrachelus nenuphar (herbst), was significantly reduced by a light tag but not by heavier tags. the m ...201020214369
[a meta analysis on randomized controlled trials of acupuncture treatment of chronic fatigue syndrome].to assess the effectiveness of acupuncture treatment of chronic fatigue syndrome (cfs).200920209981
type of snack influences satiety responses in adult women.the effect of different snack foods on satiety and plasma glucose and hormone responses was assessed. nineteen fasted adult women (mean age: 39.2 + or - 0.7 years, mean bmi: 26.1 + or - 0.8 kg/m(2)) consumed test foods including dried plums, low-fat cookies, white bread and water only on separate days. the test foods (with the exception of water) provided 238 kcal and were similar in total carbohydrate, fat and protein content but differed in fiber and sugar content. subjects rated their feeling ...201020206217
regulation of two germin-like protein genes during plum fruit development.germin-like proteins (glps) have several proposed roles in plant development and defence. two novel genes (ps-glp1 and 2) encoding germin-like protein were isolated from plum (prunus salicina). their regulation was studied throughout fruit development and during ripening of early and late cultivars. these two genes exhibited similar expression patterns throughout the various stages of fruit development excluding two important stages, pit hardening (s2) and fruit ripening (s4). during fruit devel ...201020202999
reduction of campylobacter jejuni and campylobacter coli in poultry skin by fruit extracts.campylobacter spp. are a major cause of foodborne bacterial gastroenteritis in humans, and current methods to control campylobacter contamination in foods are not completely successful. plants are a promising source of antimicrobial agents, particularly given the growing interest in "all natural" foods. in this study, the antimicrobial activity of extracts from 28 edible plants against campylobacter jejuni and campylobacter coli was evaluated in vitro and in a poultry skin model. nine of 28 extr ...201020202332
prebiotic effect of fruit and vegetable shots containing jerusalem artichoke inulin: a human intervention study.the present study aimed to determine the prebiotic effect of fruit and vegetable shots containing inulin derived from jerusalem artichoke (ja). a three-arm parallel, placebo-controlled, double-blind study was carried out with sixty-six healthy human volunteers (thirty-three men and thirty-three women, age range: 18-50 years). subjects were randomised into three groups (n 22) assigned to consume either the test shots, pear-carrot-sea buckthorn (pcs) or plum-pear-beetroot (ppb), containing ja inul ...201020187995
purunusides a-c, alpha-glucosidase inhibitory homoisoflavone glucosides from prunus domestica.purunusides a-c (1-3), new homoisoflavone glucosides together with the known compounds beta-sitosterol (4) and 6,7-methylenedioxy-8-methoxycoumarin (5) have been isolated from n-butanol and ethyl acetate soluble fractions of prunus domestica. their structures were assigned on the basis of spectral studies. the compounds 1-3 showed potent inhibitory activity against the enzyme alpha-glucosidase.201020162398
combining fructooligosaccharide and dried plum has the greatest effect on restoring bone mineral density among select functional foods and bioactive compounds.functional foods and/or their bioactive compounds playing a role in improving skeletal health have received considerable attention. the objective of the present study was to determine the extent to which certain functional foods as (1) whole, e.g., dried plum (dp), figs, dates, raisin, and blueberry, (2) fractionated, e.g., dp puree, dp juice, and dp pulp/skin, or (3) isolated, e.g., dp polyphenols, fructooligosaccharides (fos), and beta-hydroxy-beta-methylbutyrate, forms reverse bone loss in an ...201020132045
total folate: diversity within fruit varieties commonly consumed in india.folate concentrations in selected fruits were measured using the trienzyme extraction and microbiological assay with lactobacillus casei (subsp. rhamnosus) as an assay organism. fruits were purchased from different retail outlets at coimbatore, tamilnadu, india and were analyzed for total folate content. the folate content in all fruits varied considerably on a fresh weight basis from 10 to 328 microg/100 g, with tropical fruits ranging between 10 and 211 microg/100 g, temperate fruits from 11 t ...201020128636
compositional changes in cell wall polysaccharides from japanese plum ( prunus salicina lindl.) during growth and on-tree ripening.climacteric japanese plums were harvested at six developmental stages with no intermediate storage period, and cell wall compositional changes were analyzed. arabinose proved to be the principal neutral monosaccharide constituent in cell walls during growth and the most dynamic neutral sugar in pectic fractions. arabinose loss from tightly bound pectins was found to be a relatively early feature in the sequence of cell wall biochemical modifications, thus suggesting a softening-related role duri ...201020099830
application of feces extracts and synthetic analogues of the host marking pheromone of anastrepha ludens significantly reduces fruit infestation by a. obliqua in tropical plum and mango backyard orchards.we determined the efficacy of three potential oviposition deterrents in reducing fruit infestation by anastrepha obliqua in tropical plum and mango orchards. these were: (1) extracts of feces of mexican fruit fly, anastrepha ludens, known to contain the a. ludens host marking pheromone (hmp) and (2) two fully synthetic simplified analogues of the naturally occurring compound, which we have named desmethyl a. ludens hmp (dm-hmp) and anastrephamide. two applications of feces extracts 2 or 3 wk bef ...200920069857
cultivated grapevines represent a symptomless reservoir for the transmission of hop stunt viroid to hop crops: 15 years of evolutionary analysis.hop stunt was a mysterious disorder that first emerged in the 1940s in commercial hops in japan. to investigate the origin of this disorder, we infected hops with natural hop stunt viroid (hpsvd) isolates derived from four host species (hop, grapevine, plum and citrus), which except for hop represent possible sources of the ancestral viroid. these plants were maintained for 15 years, then analyzed the hpsvd variants present. here we show that the variant originally found in cultivated grapevines ...200920041179
putative recombination events and evolutionary history of five economically important viruses of fruit trees based on coat protein-encoding gene sequence analysis.to enhance the knowledge of recombination as an evolutionary process, 267 accessions retrieved from genbank were investigated, all belonging to five economically important viruses infecting fruit crops (plum pox, apple chlorotic leaf spot, apple mosaic, prune dwarf, and prunus necrotic ringspot viruses). putative recombinational events were detected in the coat protein (cp)-encoding gene using recco and rdp version 3.31beta algorithms. based on recco results, all five viruses were shown to conta ...201020035376
attraction of anoplophora glabripennis to male-produced pheromone and plant volatiles.the male-produced pheromone of anoplophora glabripennis (motschulsky) (coleoptera: cerambycidae), which is an equal blend of 4-(n-heptyloxy)butan-1-ol and 4-(n-heptyloxy)butanal, was used in laboratory bioassays and in the greenhouse to determine its potential for attracting a. glabripennis adults. in modified "walking wind tunnels," virgin females were most attracted to the alcohol component, and virgin males were repelled by the pheromone blend at the lowest and highest amounts offered. y-tube ...200920021771
redesigning a large-scale clinical trial in response to negative external trial results: the camus study of phytotherapy for benign prostatic hyperplasia.benign prostatic hyperplasia (bph), a common condition among older men, confers its morbidity through potentially bothersome lower urinary tract symptoms. treatments for bph include drugs such as alpha-adrenergic receptor blockers and 5-alpha reductase inhibitors, minimally invasive therapies that use heat to damage or destroy prostate tissue, and surgery including transurethral resection of the prostate. complementary and alternative medicines are gaining popularity in the us. two phytotherapie ...200920007408
[observation on therapeutic effect of electroacupuncture at jiaji (ex-b 2) combined with blood-letting and cupping on herpes zoster].to compare the therapeutic effect differences between electroacupuncture at jiaji (ex-b 2) combined with blood-letting plus cupping and western medicine therapy.200919994687
siadenovirus infection in two psittacine bird species.consensus polymerase chain reaction was used to identify a novel adenovirus from two psittacine birds: a plum-headed parakeet (psittacula cyanocephala) with lethargy, weight loss, and marked leukocytosis; and an umbrella cockatoo (cacatua alba) with lethargy, weight loss, and feather abnormalities. phylogenetic and comparative sequence analysis suggested that this virus is a member of the genus siadenovirus, and is here termed psittacine adenovirus 2. this extends the characterized adenoviruses ...200919937528
structural determination of prunusins a and b, new c-alkylated flavonoids from prunus domestica, by 1d and 2d nmr spectroscopy.prunusins a (1) and b (2), the new c-alkylated flavonoids, have been isolated from the seed kernels of prunus domestica. their structures were assigned from (1)h and (13)c nuclear magnetic resonating spectra, dept and by correlation spectroscopy, hmqc and hmbc experiments. 3, 5, 7, 4'-tetrahydroxyflavone (3) and 3, 5, 7-trihydroxy-8, 4'-dimethoxyflavone (4) have also been reported from this species. both compounds (1) and (2) showed significant antifungal activity against pathogenic fungus trich ...201019918802
analysis of phenolic compounds in six norwegian plum cultivars (prunus domestica l.).six european plum cultivars ( prunus domestica l.) grown in norway have been studied with respect to phenolic composition. neochlorogenic acid was found to be the most important phenolic acid in all cultivars. together with other phenolic acids, this compound varied significantly in amount among the cultivars. cyanidin 3-rutinoside was found to account for >60% of the total anthocyanin content. minor amounts of flavonols (rutin and quercetin 3-glucoside) were detected in all cultivars. total ant ...200919888727
curative activity of insecticides against plum curculio (coleoptera: curculionidae) in tart cherries.tart cherry, prunus cerasus l. variety montmorency, fruit were infested with plum curculio, conotrachelus nenuphar (herbst) (coleoptera: curculionidae), and treated with insecticides to target late instars, neonates, and eggs. the organophosphates azinphos-methyl and phosmet and the neonicotinoid thiamethoxam reduced larval emergence rates by >90% for all life stage targets; after >30 d, few surviving larvae were found inside fruit. acetamiprid and thiacloprid also had curative activity and yiel ...200919886451
a tale of two carboxypeptidases.proopiomelanocortin (pomc) neurons play a central role in energy homeostasis. despite the complexity of pomc posttranslational processing, regulation of pomc gene expression often takes center stage. complementary papers that zero in on distinct carboxypeptidases (plum et al., 2009; wallingford et al., 2009) now refocus the spotlight on regulated peptide cleavage.200919883612
measurement of greenhouse gas emissions from agricultural sites using open-path optical remote sensing method.improved characterization of distributed emission sources of greenhouse gases such as methane from concentrated animal feeding operations require more accurate methods. one promising method is recently used by the usepa. it employs a vertical radial plume mapping (vrpm) algorithm using optical remote sensing techniques. we evaluated this method to estimate emission rates from simulated distributed methane sources. a scanning open-path tunable diode laser was used to collect path-integrated conce ...200919827493
effectiveness of glues used for harmonic radar tag attachment and impact on survival and behavior of three insect pests.the ability of three cyanoacrylate glues to ensure a durable bond between the colorado potato beetle, leptinotarsa decemlineata (say), the plum curculio, conotrachelus nenuphar (herbst), or the corn rootworms (western corn rootworm, diabrotica virgifera virgifera leconte and northern corn rootworm, diabrotica longicornis smith and lawrence) and the harmonic radar tag without impact on behavior and survival was assessed as part of a study on the use of harmonic radar technology to track these ins ...200919791611
pesticide extraction from table grapes and plums using ionic liquid based dispersive liquid-liquid microextraction.room temperature ionic liquids (rtils) have been used as extraction solvents in dispersive liquid-liquid microextraction (dllme) for the determination of eight multi-class pesticides (i.e. thiophanate-methyl, carbofuran, carbaryl, tebuconazole, iprodione, oxyfluorfen, hexythiazox, and fenazaquin) in table grapes and plums. the developed method involves the combination of dllme and high-performance liquid chromatography with diode array detection. samples were first homogenized and extracted with ...200919779926
geographical location has greater impact on carotenoid content and bioaccessibility from tomatoes than variety.the suggested health benefits of consuming tomatoes and tomato-based products have been attributed, in part, to the carotenoids present in these foods. therefore, the objectives of the present study were to (i) analyse carotenoid content and bioaccessibility from different tomato (lycopersicon esculentum l.) types namely cherry, plum, round, and certain tomatoes-on-the-vine; and (ii) determine if geographical location (ireland vs spain) influenced the content and bioaccessibility of carotenoids ...200919757067
a new selective medium for the recovery and enumeration of monilinia fructicola, m. fructigena, and m. laxa from stone fruits.isolation of monilinia spp. from stone and pome fruit surfaces is difficult due to the presence of several fast-growing fungal species such as rhizopus, alternaria, and penicillium spp. therefore, a new selective medium (acidified potato dextrose agar [ph 3.6] amended with fosetyl-aluminum [fosetyl-al] at 500 microg/ml) (apda-f500) was developed for the recovery of monilinia propagules. the antifungal agents fosetyl-al, dichloran, ammonium molybdate, and 2-deoxy-d-glucose (2-dd-glucose) were tes ...200919740034
the determinant of potyvirus ability to overcome the rtm resistance of arabidopsis thaliana maps to the n-terminal region of the coat protein.in arabidopsis thaliana columbia (col-0) plants, the restriction of tobacco etch virus (tev) long-distance movement involves at least three dominant rtm (restricted tev movement) genes named rtm1, rtm2, and rtm3. previous work has established that, while the rtm-mediated resistance is also effective against other potyviruses, such as plum pox virus (ppv) and lettuce mosaic virus (lmv), some isolates of these viruses are able to overcome the rtm mechanism. in order to identify the viral determina ...200919737103
towards the routine use of brain imaging to aid the clinical diagnosis of disorders of consciousness.clinical audits have highlighted the many challenges and dilemmas faced by clinicians assessing persons with disorders of consciousness (vegetative state and minimally conscious state). the diagnostic decision-making process is highly subjective, dependent upon the skills of the examiner and invariably dictated by the patients' ability to move or speak. whilst a considerable amount has been learnt since jennett and plum coined the term 'vegetative state', the assessment process remains largely u ...200919710182
field efficacy of entomopathogenic fungi and nematodes targeting caged last-instar plum curculio (coleoptera: curculionidae) in michigan cherry and apple orchards.the plum curculio (conotrachelus nenuphar herbst) is a key pest of pome and stone fruit in eastern north america. we tested the efficacy of five pathogens over the course of three seasons in 10 michigan apple and cherry orchards, with introductions of larvae to caged pots containing pathogen-treated soil. the nematode steinernema riobrave was the most effective pathogen in the 2 yr it was tested, but only in soils with the highest sand content (81-88%) and when it was applied 1 h or 5 d after la ...200919689891
quality of life in simultaneous pancreas-kidney transplant recipients.simultaneous pancreas-kidney transplantation (spk tx) allows dialysis and insulin therapy to be discontinued and improves the complications of diabetes mellitus type 1 (dm1). this study measure quality of life (qol) in spk transplant recipients and determine if there are differences in qol between these patients and those with dm1 in renal replacement therapy (rrt).200919674015
analysis of pesticide residues by fast gas chromatography in combination with negative chemical ionization mass spectrometry.a combination of fast gc with narrow-bore column and bench top quadrupole mass spectrometer (ms) detector in negative chemical ionization (nci) mode (with methane as reagent gas) is set up and utilized for the ultratrace analysis of 25 selected pesticides. the observed pesticides, belonging to the endocrine disrupting chemicals (edcs), were from different chemical classes. a comparative study with electron impact (ei) ionization was also carried out (both techniques in selected ion monitoring (s ...200919643423
induction of apoptosis by immature plum in human hepatocellular carcinoma.in the present study, the effect of an extract of immature prunus salicina lindl. cv. soldam fruit on the viability and induction of apoptosis in human hepatocellular carcinoma hepg2 cells was investigated. the results showed that in comparison with other cancer cells, the growth inhibition exerted by immature plum extracts was greatest in hepg2. apoptosis in hepg2 cells mediated by immature plums was associated with "death receptor signaling." immature plum extracts significantly increased the ...200919627199
lower limb deformity due to failed trauma treatment corrected with the ilizarov technique: factors affecting the complication rate in 52 patients.failed treatment of fractures may be corrected by the ilizarov technique but complications are common. in 52 patients with compromised healing of femoral and tibial fractures, the results of secondary reconstruction with ilizarov treatment were investigated retrospectively in order to identify the factors that contribute to the risk of complications.200919626469
[parasitoids (hymenoptera: braconidae) of fruit flies (diptera: tephritidae) in the state of são paulo, brazil: associated plants and parasitism].a total of 3,008 braconid parasitoids in 26 species of fruit fly host plants were collected in 33 localities of são paulo state. the majority of the braconids (96.2%) belonged to four opiinae species, doryctobracon areolatus (szépligeti), doryctobracon brasiliensis (szépligeti), opius bellus (gahan) and utetes anastrephae (viereck), and the remaining 3.8%, a single species of alysiinae, asobara anastrephae (muesebeck). doryctobracon areolatus was collected on anastrepha fraterculus (wied), a. ob ...200919618046
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