Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
comprehensive analysis of expressed sequence tags from cultivated and wild radish (raphanus spp.).radish (raphanus sativus l., 2n = 2× = 18) is an economically important vegetable crop worldwide. a large collection of radish expressed sequence tags (ests) has been generated but remains largely uncharacterized.201324144082
bioactive metabolites isolated from penicillium sp. yy-20, the endophytic fungus from ginkgo biloba.six known metabolites, adenosine (1), methyl β-d-ribofuranoside (2), adenine (3), 2'-deoxyadenosine (4), 3-methylpiperazine-2,5-dione (5) and 2'-deoxyuridine (6), were isolated from the extracts of the endophytic fungus penicillium sp. yy-20 isolated from the root of ginkgo biloba, and their structures were elucidated by spectroscopic methods. the antioxidant and growth-promoting activities of these compounds were first evaluated. the results indicated that compounds 1, 3 and 4 exhibited potenti ...201424144081
tandem combination of trigonella foenum-graecum defensin (tfgd2) and raphanus sativus antifungal protein (rsafp2) generates a more potent antifungal protein.plant defensins are small (45 to 54 amino acids) positively charged antimicrobial peptides produced by the plant species, which can inhibit the growth of a broad range of fungi at micro-molar concentrations. these basic peptides share a common characteristic three-dimensional folding pattern with one α-helix and three β-sheets that are stabilized by eight disulfide-linked cysteine residues. instead of using two single-gene constructs, it is beneficial when two effective genes are made into a sin ...201324022215
enhanced reduction of phenol content and toxicity in olive mill wastewaters by a newly isolated strain of coriolopsis gallica.the search for novel microorganisms able to degrade olive mill wastewaters (omw) and withstand the toxic effects of the initially high phenolic concentrations is of great scientific and industrial interest. in this work, the possibility of reducing the phenolic content of omw using new isolates of fungal strains (coriolopsis gallica, bjerkandera adusta, trametes versicolor, trichoderma citrinoviride, phanerochaete chrysosporium, gloeophyllum trabeum, trametes trogii, and fusarium solani) was inv ...201423979847
rare earth elements (rees): effects on germination and growth of selected crop and native plant species.the phytotoxicity of rare earth elements (rees) is still poorly understood. the exposure-response relationships of three native canadian plant species (common milkweed, asclepias syriaca l., showy ticktrefoil, desmodium canadense (l.) dc. and switchgrass, panicum virgatum l.) and two commonly used crop species (radish, raphanus sativus l., and tomato, solanum lycopersicum l.) to the rees lanthanum (la), yttrium (y) and cerium (ce) were tested. in separate experiments, seven to eight doses of eac ...201423978671
phytotoxicity of new furan-derived aminophosphonic acids, n-aryl furaldimines and 5-nitrofuraldimine.the aim of this work was to synthesize selected furaldimines and their aminophosphonic derivatives and evaluation the phytotoxicity of new obtained products according to oecd 208 guideline. four schiff bases, n-furfurylidene-p-anisidine (1a), n-furfurylidene-p-toluidine (1b), n-furfurylidene-benzhydrylamine (1c), and n-(2-nitrofurfurylidene)-p-toluidine (1d) were synthesized and three new furan-derived n-substituted aminomethylphosphonic acids, namely: 2-furyl n-(p-methoxyphenyl)-aminomethylphos ...201323869973
comparative mapping of raphanus sativus genome using brassica markers and quantitative trait loci analysis for the fusarium wilt resistance trait.fusarium wilt (fw), caused by the soil-borne fungal pathogen fusarium oxysporum is a serious disease in cruciferous plants, including the radish (raphanus sativus). to identify quantitative trait loci (qtl) or gene(s) conferring resistance to fw, we constructed a genetic map of r. sativus using an f2 mapping population derived by crossing the inbred lines '835' (susceptible) and 'b2' (resistant). a total of 220 markers distributed in 9 linkage groups (lgs) were mapped in the raphanus genome, cov ...201323864230
phytotoxicity of secondary metabolites isolated from flourensia oolepis s.f.blake.the aim of this study was to isolate the active principles of flourensia oolepis s.f.blake (asteraceae), which completely inhibited the germination of raphanus sativus seeds at 10 mg/ml. flavanone pinocembrin and sesquiterpene ilicol, were isolated by bioassay-guided fractionation. they were active both against monocot and dicot seeds. pinocembrin was the most active compound, with an ic50 (germination) value of 0.24, 3.40, 3.28, and 3.55 mm against panicum miliaceum, avena sativa, lactuca sativ ...201323847074
a new cryptic virus belonging to the family partitiviridae was found in watermelon co-infected with melon necrotic spot virus.a novel virus was detected in watermelon plants (citrullus lanatus thunb.) infected with melon necrotic spot virus (mnsv) using solid next-generation sequence analysis. in addition to the expected msnv genome, two double-stranded rna (dsrna) segments of 1,312 and 1,118 bp were also identified and sequenced from the purified virus preparations. these two dsrna segments encode two putative partitivirus-related proteins, an rna-dependent rna polymerase (rdrp) and a capsid protein, which were sequen ...201323775759
comparison of bioactive phytochemical content and release of isothiocyanates in selected brassica sprouts.the consumption of brassica sprouts as raw vegetables provides a fair amount of glucosinolates (gls) and active plant myrosinase, which enables the breakdown of gls into health-promoting isothiocyanates (itcs). this study reports the determination of the main constituents related to human health found in edible sprouts of two brassica oleracea varieties, broccoli and tuscan black kale, and two raphanus sativus varieties, daikon and sango. radish sprouts exhibited the highest ability to produce i ...201323768361
antifungal, insecticidal, and plant growth promoting potential of streptomyces hydrogenans dh16.in the present study, an actinobacterium strain, possessing antagonistic activity against different fungal phytopathogens viz. colletotrichum acutatum, cladosporium herbarum, alternaria brassicicola, exserohilum sp., alternaria mali, colletotrichum gleospoiroides, alternaria alternata, cercospora sp., fusarium oxysporum f.sp. dianthi and fusarium moniliformae, was isolated from soil and identified as streptomyces hydrogenans dh16. application of culture supernatant (5%)/cells (10(7)  cfu ml(-1) ...201423765423
development of a chloroplast dna marker for monitoring of transgene introgression in brassica napus l.chloroplast molecular markers can provide useful information for high-resolution analysis of inter- and intra-specific variation in brassicaceae and for differentiation between its species. combining data generated from nuclear and chloroplast markers enables the study of seed and pollen movement, and assists in the assessment of gene-flow from genetically modified (gm) plants through hybridization studies. to develop chloroplast dna markers for monitoring of transgene introgression in brassica ...201323690044
deciphering the diploid ancestral genome of the mesohexaploid brassica rapa.the genus brassica includes several important agricultural and horticultural crops. their current genome structures were shaped by whole-genome triplication followed by extensive diploidization. the availability of several crucifer genome sequences, especially that of chinese cabbage (brassica rapa), enables study of the evolution of the mesohexaploid brassica genomes from their diploid progenitors. we reconstructed three ancestral subgenomes of b. rapa (n = 10) by comparing its whole-genome seq ...201323653472
special plant species determines diet breadth of phytophagous insects: a study on host plant expansion of the host-specialized aphis gossypii glover.host specialization is a ubiquitous character of phytophagous insects. the polyphagous population is usually composed of some subpopulations that can use only a few closely related plants. cotton-melon aphids, aphis gossypii glover exhibited strong host specialization, and the cotton- and cucurbits-specialized biotypes had been clearly identified. however, the experimental work that addressed the roles of plant species in determining diet breadth of phytophagous insects is rare. in the present s ...201323580128
yield, chemical composition and nutritional quality responses of carrot, radish and turnip to elevated atmospheric carbon dioxide.future concentration of carbon dioxide in the atmosphere is very important due to its apparent economic and environmental impact in terms of climate change. however, a compressive assessment of its effect on the nutritional and chemical characteristics of food crops has yet to be established. in the present study the impact of elevated atmospheric co2 on the yield, chemical composition and nutritional quality of three root vegetables, carrot (daucus carota l. cv. t-1-111), radish (raphanus sativ ...201323576218
chemical composition and biological activities of the essential oils from three melaleuca species grown in tunisia.the chemical composition of the essential oils of melaleuca armillaris sm., melaleuca styphelioides sm. and melaleuca acuminata f. muell., collected in tunisia, was studied by means of gc and gc-ms analysis. in all, 46 compounds were identified, 38 for m. armillaris, 20 for m. acuminata and eight for m. styphelioides, respectively. the presence of a sesquiterpenic fraction (52.2%) characterized the oil from m. armillaris; m. sthypheliodes oil was rich in methyl eugenol, a phenolic compound (91.1 ...201223443119
chemical composition and antimicrobial and allelopathic activity of tunisian conyza sumatrensis (retz.) e.walker essential oils.conyza sumatrensis (retz.) e.walker (asteraceae) is a spontaneous annual herb, fairly widespread throughout tunisia, which has rarely been studied or valued in any sector. essential oils were obtained by hydrodistillation of different parts (flower heads, leaves, stems, and roots) of c. sumatrensis plants, which were collected in autumn (november 2007) at the flowering stage in the area of monastir, tunisia. in total, 98 compounds, representing 88.1-99.3% of the oil composition, were identified ...201323418168
nucleotide sequence variation of glabra1 contributing to phenotypic variation of leaf hairiness in brassicaceae vegetables.glabra1 (gl1) belongs to the group of r2r3-myb transcription factors and is known to be essential for trichome initiation in arabidopsis. in our previous study, we identified a gl1 ortholog in brassica rapa as a candidate for the gene controlling leaf hairiness by qtl analysis and suggested that a 5-bp deletion (b-allele) and a 2-bp deletion (d-allele) in the exon 3 of brgl1 and a non-synonymous snp (c-allele) in the second nucleotide of exon 3 possibly cause leaf hairlessness. in this study, we ...201323370467
qtl analysis using snp markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, raphanus sativus l.snp markers for qtl analysis of 4-mtb-gsl contents in radish roots were developed by determining nucleotide sequences of bulked pcr products using a next-generation sequencer. dna fragments were amplified from two radish lines by multiplex pcr with six primer pairs, and those amplified by 2,880 primer pairs were mixed and sequenced. by assembling sequence data, 1,953 snps in 750 dna fragments, 437 of which have been previously mapped in a linkage map, were identified. a linkage map of nine linka ...201323308250
microbiological investigation of raphanus sativus l. grown hydroponically in nutrient solutions contaminated with spoilage and pathogenic bacteria.the survival of eight undesired (spoilage/pathogenic) food related bacteria (citrobacter freundii pss60, enterobacter spp. pss11, escherichia coli pss2, klebsiella oxytoca pss82, serratia grimesii pss72, pseudomonas putida pss21, stenotrophomonas maltophilia pss52 and listeria monocytogenes atcc 19114(t)) was investigated in mineral nutrient solution (mns) during the crop cycle of radishes (raphanus sativus l.) cultivated in hydroponics in a greenhouse. mnss were microbiologically analyzed weekl ...201223290244
molecular cloning and functional expression of a water-soluble chlorophyll-binding protein from japanese wild radish.hydrophilic chlorophyll (chl)-binding proteins have been isolated from various brassicaceae plants and are categorized into class ii water-soluble chl-binding proteins (wscps). although the molecular properties of class ii wscps including brassica-type (e.g., cauliflower wscp, brussels sprouts wscp and bnd22, a drought- and salinity-stress-induced 22 kda protein of rapeseed), a lepidium-type, and an arabidopsis-type wscps have been well characterized, those of raphanus-type wscps are poorly unde ...201323266282
phytotoxic activity of foliar volatiles and essential oils of calamintha nepeta (l.) savi.foliar volatiles and essential oils of calamintha nepeta (l.) savi, a mediterranean plant species belonging to the labiatae family, were investigated for their phytotoxic activities on seed germination and root growth of crops (lactuca sativa l. and raphanus sativus l.) and weed species (lolium perenne l. and amaranthus retroflexus l.). foliar volatiles of c. nepeta (l.) savi strongly inhibited both germination and root growth of lettuce, and its essential oils, especially at 125, 250 and 500 μl ...201323216166
genetic analysis of hybrid seed formation ability of brassica rapa in intergeneric crossings with raphanus sativus.a hybridization barrier leads to the inability of seed formation after intergeneric crossings between brassica rapa and raphanus sativus. most b. rapa lines cannot set intergeneric hybrid seeds because of embryo breakdown, but a b. rapa line obtained from turnip cultivar 'shogoin-kabu' is able to produce a large number of hybrid seeds as a maternal parent by crossings with r. sativus. in 'shogoin-kabu' crossed with r. sativus, developments of embryos and endosperms were slower than those in intr ...201323203221
complete sequence of heterogenous-composition mitochondrial genome (brassica napus) and its exogenous source.unlike maternal inheritance of mitochondria in sexual reproduction, somatic hybrids follow no obvious pattern. the introgressed segment orf138 from the mitochondrial genome of radish (raphanus sativus) to its counterpart in rapeseed (brassica napus) demonstrates that this inheritance mode derives from the cytoplasm of both parents. sequencing of the complete mitochondrial genome of five species from brassica family allowed the prediction of other extraneous sources of the cybrids from the radish ...201223190559
a real-scale soil phytoremediation.in the present investigation, a phytoremediation process with a combination of different plant species (populus nigra (var.italica), paulownia tomentosa and cytisus scoparius), and natural growing vegetation has been proposed at real-scale (10.000 m(2)) to bioremediate and functionally recover a soil historically contaminated by heavy metals and hydrocarbons. in the attempts to assess both effectiveness and evolution of the remediation system towards a natural soil ecosystem, besides the polluti ...201323179352
carbonaceous soil amendments to biofortify crop plants with zinc.carbonaceous soil amendments, comprising mixtures of biosolids and biochar, have been demonstrated to improve fertility while reducing nitrate leaching. we aimed to determine the efficacy of a biosolids/biochar soil amendment in biofortification of vegetables with zn, an element that is deficient in one third of humanity. we grew beetroot (beta vulgaris), spinach (spinacia oleracea), radish (raphanus sativus), broccoli (brassica oleracea), carrot (daucus carota), leek (allium ampeloprsum), onion ...201323146312
development of genomic and est-ssr markers in radish (raphanus sativus l.).radish (raphanus sativus l.) belongs to brassicaceae family and is a close relative of brassica. this species shows a wide morphological diversity, and is an important vegetable especially in asia. however, molecular research of radish is behind compared to that of brassica. for example, reports on ssr (simple sequence repeat) markers are limited. here, we designed 417 radish ssr markers from ssr-enriched genomic libraries and the cdna data. of the 256 ssr markers succeeded in pcr, 130 showed cl ...201123136479
composting of waste paint sludge containing melamine resin and the compost's effect on vegetable growth and soil water quality.melamine resin (mr) is introduced to the environment from many industrial effluents, including waste paint sludge (wps) from the automobile industry. melamine resin contains a high nitrogen (n) content and is a potential n source during composting. in this study, two carbon sources, waste paper (wp) and plant residue (pr), were used to study their effects on composting of wps. additional work tested the wps-composts effects on plant growth and soil water quality. after 84 days of composting, 85% ...201223127275
increased resistance to late leaf spot disease in transgenic peanut using a combination of pr genes.peanut (arachis hypogaea l.) is the sixth most important oil seed crop in the world. yield loss due to cercospora leaf spot (early and late leaf spots) is a serious problem in cultivating this crop. non-availability of resistant genes within crossable germplasms of peanut necessitates the use of a genetic engineering strategy to develop genetic resistance against various biotic stresses. the pathogenesis-related (pr) proteins are a group of plant proteins that are toxic to invading fungal pathog ...201223053199
phytotoxic effect of 2-benzoxazolinone (boa) against some vegetable crops.benzoxazolin-2(3h)-one (boa) is a well known allelochemical that is being explored for its herbicidal activity. however, not much is known about its effect on crop plants. the present study investigated the effect of boa on germination and early growth of four vegetable crops viz. pisum sativum l., raphanus sativus l., brassica oleracea l. var. botrytis and brassica oleracea l. var. capitata. at 1000 microm, germination of p. sativum, r. sativus and b. oleracea var. botrytis was reduced by more ...201223033638
effects of selenium accumulation on phytotoxicity, herbivory, and pollination ecology in radish (raphanus sativus l.).selenium (se) has contaminated areas in the western usa where pollination is critical to the functioning of both agricultural and natural ecosystems, yet we know little about how se can impact pollinators. in a two-year semi-field study, the weedy plant raphanus sativus (radish) was exposed to three selenate treatments and two pollination treatments to evaluate the effects on pollinator-plant interactions. honey bee (apis mellifera l.) pollinators were observed to readily forage on r. sativus fo ...201323000967
interaction of brassinosteroids and polyamines enhances copper stress tolerance in raphanus sativus.brassinosteroids (brs) and polyamines (pas) regulate various responses to abiotic stress, but their involvement in the regulation of copper (cu) homeostasis in plants exposed to toxic levels of cu is poorly understood. this study provides an analysis of the effects of exogenously applied brs and pas on radish (raphanus sativus) plants exposed to toxic concentrations of cu. the interaction of 24-epibrassinolide (ebr, an active br) and spermidine (spd, an active pa) on gene expression and the phys ...201222915739
acquiring transgenic tobacco plants with insect resistance and glyphosate tolerance by fusion gene transformation.the advantages of gene 'stacking' or 'pyramiding' are obvious in genetically modified (gm) crops, and several different multi-transgene-stacking methods are available. using linker peptides for multiple gene transformation is considered to be a good method to meet a variety of needs. in our experiment, the bt cry1ah gene, which encodes the insect-resistance protein, and the mg ( 2 ) -epsps gene, which encodes the glyphosate-tolerance protein, were connected by a 2a or lp4/2a linker. linker 2a is ...201222777591
insecticide resistance mechanisms in the green peach aphid myzus persicae (hemiptera: aphididae) ii: costs and benefits.among herbivorous insects that have exploited agro-ecosystems, the peach-potato aphid, myzus persicae, is recognized as one of the most important agricultural pests worldwide. uses over 400 plant species and has evolved different insecticides resistance mechanisms. as m. persicae feeds upon a huge diversity of hosts, it has been exposed to a wide variety of plant allelochemicals, which probably have promoted a wide range of detoxification systems.201222685539
[eco-hydrological characteristics and soil and water conservation effect of citrus plantation on slope red soil of jiangxi province, china].a 9-year observation was conducted at the experimental plots in the citrus reticulata plantation in jiangxi provincial eco-technology park to study the eco-hydrological characteristics and soil conservation benefits of the plantation on slope red soil. seven treatments were designed and monitored over nine years. the average flow and the rate of sediment for the seven treatments were reduced by 78.5% and 77.2%, respectively. the reduction rates were the highest in treatments band coverage of pas ...201222586974
physiology and bio - chemistry of germination of different types of seeds - iv - effect of certain chemicals on growth anddevelopment of cucumber, mungo, paddy, raddish and tomato plants.the effect of 100 ppm solution of each of kinetin, adenine, uracil and thymine on the vegetative and reproductive growth of cucumis sativus, phaseolus mungo, oryza sativus, raphanus sativus and lycopersicum esculentum plants were studied. the rate of vegetative growth was found to be more in the cucumis sativus, raphanus sativus and lycopersicum esculentum plants treated with all the chemicals mentioned above over that of the controls. phaseolus mungo and oryza sativa plants shows almost the sam ...198422557413
contractile effect of radish and betel nut extracts on rabbit gallbladder.raphanus sativus (abbreviated in this paper as rs.cr) and areca catechu (ac.cr), commonly known as radish and betel nut respectively, are traditionally used in south asia for different gastrointestinal, gallbladder, and hepatic diseases. there has not been any study to see how they modulate gallbladder contractility. we selected isolated rabbit gallbladder tissue preparations, mounted them in tissue baths containing krebs-henseleit solution at 37°c, and then recorded the changes in baseline tone ...201222499720
relationship between pollutant content and ecotoxicity of sewage sludges from spanish wastewater treatment plants.chemical and ecotoxicological properties of 28 sewage sludge samples from spanish wastewater treatment plants were studied in order to assess their suitability for agricultural purposes. sludge samples were classified into five categories according to specific treatment processes in terms of digestion (aerobic/anaerobic) and drying (mechanical/thermal). composted samples, as indicative of the most refined process, were also considered. sludges were subjected to physical-chemical characterization ...201222483948
biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in india.emerging whitefly transmitted begomoviruses are major pathogens of vegetable and fibre crops throughout the world, particularly in tropical and sub-tropical regions. mutation, pseudorecombination and recombination are driving forces for the emergence and evolution of new crop-infecting begomoviruses. leaf curl disease of field grown radish plants was noticed in varanasi and pataudi region of northern india. we have identified and characterized two distinct monopartite begomoviruses and associate ...201222339942
mes16, a member of the methylesterase protein family, specifically demethylates fluorescent chlorophyll catabolites during chlorophyll breakdown in arabidopsis.during leaf senescence, chlorophyll (chl) is broken down to nonfluorescent chlorophyll catabolites (nccs). these arise from intermediary fluorescent chlorophyll catabolites (fccs) by an acid-catalyzed isomerization inside the vacuole. the chemical structures of nccs from arabidopsis (arabidopsis thaliana) indicate the presence of an enzyme activity that demethylates the c13(2)-carboxymethyl group present at the isocyclic ring of chl. here, we identified this activity as methylesterase family mem ...201222147518
effects of chinese herbal cataplasm xiaozhang tie on cirrhotic ascites.conventional methods of treating cirrhotic ascites are inadequate. we sought to identify a novel, effective approach to relieve the suffering of patients with cirrhotic ascites.201122107835
influence of various host plants on the consumption and utilization of food by pieris brassicae (linn.).pieris brassicae (linn.) is a destructive cosmopolitan pest of cruciferous crops. it is present wherever its host plants occur, and it is considered to be one of the most widely distributed of all the lepidoptera. we investigated the affect of various host plants on the food consumption and utilization by p. brassicae. we quantified consumption of food, larval duration, pupal duration and weight on cabbage (brassica oleracea var. capitata), cauliflower (brassica oleracea var. botrytis), radish ( ...201122030306
Autotoxicity and allelopathy of 3,4-dihydroxyacetophenone isolated from Picea schrenkiana needles.Bioassay-guided fractionation of the diethyl ether fraction of a water extract of Picea schrenkiana needles led to the isolation of the phenolic compound 3,4-dihydroxy- acetophenone (DHAP). The allelopathic effects of DHAP were evaluated under laboratory conditions on P. schrenkiana, rice (Oryza sativa L.), wheat (Triticum aestivum L.), radish (Raphanus sativus L.), lettuce (Latuca sativa L.), cucumber (Cucumis sativus L.) and mung bean (Phaseolus radiatus L.). DHAP significantly inhibited seed ...201122024957
Radish (Raphanus sativus L. leaf) ethanol extract inhibits protein and mRNA expression of ErbB(2) and ErbB(3) in MDA-MB-231 human breast cancer cells.In this study, we investigated the effects of the ethanol extract of aerial parts of Raphanus sativus L. (ERL) on breast cancer cell proliferation and gene expression associated with cell proliferation and apoptosis in MDA-MB-231 human breast cancer cells. The MDA-MB-231 cells were cultured in the presence or absence of various concentrations (100, 200, or 300 µg/mL) of ERL. ERL significantly decreased cell proliferation after 48 h of incubation (P < 0.05). The protein and mRNA expression of Erb ...201121994522
genetic and pathogenic variability of indian strains of xanthomonas campestris pv. campestris causing black rot disease in crucifers.xanthomonas campestris pv. campestris (pammel) dowson (xcc) causing black rot of crucifers is a serious disease in india and causes >50% crop losses in favorable environmental conditions. pathogenic variability of xcc, x. oryzae pv. oryzae (xoo), and x. axonopodis pv. citri (xac) were tested on 19 cultivars of cruciferae including seven brassica spp. viz., b. campestris, b. carinata, b. juncea, b. napus, b. nigra, b. oleracea and b. rapa, and raphanus sativus for two consecutive years viz., 2007 ...201121956666
terrestrial ecotoxicity of short aliphatic protic ionic liquids.a study of the ecotoxicity of different short aliphatic protic ionic liquids (pils) on terrestrial organisms was conducted. tests performed within the present study include those assessing the effects of pils on soil microbial functions (carbon and nitrogen mineralization) and terrestrial plants. the results show that the nominal lowest-observed-adverse-effect concentration (loaec) values were 5,000 mg/kg (dry soil) for the plant test in two species (lolium perenne, allium cepa), 1,000 mg/kg (dr ...201121935980
extensive chromosome homoeology among brassiceae species were revealed by comparative genetic mapping with high-density est-based snp markers in radish (raphanus sativus l.).a linkage map of expressed sequence tag (est)-based markers in radish (raphanus sativus l.) was constructed using a low-cost and high-efficiency single-nucleotide polymorphism (snp) genotyping method named multiplex polymerase chain reaction-mixed probe dot-blot analysis developed in this study. seven hundred and forty-six snp markers derived from est sequences of r. sativus were assigned to nine linkage groups with a total length of 806.7 cm. by blastn, 726 markers were found to have homologous ...201121816873
toxicity of sodium tungstate to earthworm, oat, radish, and lettuce.due to unknown effects of the potential exposure of the terrestrial environment to tungsten substances, a series of toxicity studies of sodium tungstate (na(2) wo(4) ) was conducted. the effect on earthworm (eisenia fetida) survival and reproduction was examined using organisation for economic co-operation and development (oecd) guideline 222. no effect on either endpoint was seen at the highest concentration tested, resulting in a 56-d no-observed-effect concentration (noec) of ≥586 mg tungsten ...201121805499
polyphenolics profile and antioxidant properties of raphanus sativus l.raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological properties. antioxidant and radical scavenging activity of r. sativus root extracted with solvents of varying polarity were evaluated using different model systems. polyphenolic content was estimated to be in the range 13.18-63.54ôçëmgôçëg(-1) dry weight, with a considerable amount being obtained with polar solvents. high-performance liquid chromatography analysis indicated the presence of ...201121714734
properties and detection of two cryptoviruses from pepper (capsicum annuum).pepper (capsicum annuum l.) contains a range of endogenous dsrna molecules resembling the genomes of cryptoviruses. in this work, we have completed the molecular characterization of pepper cryptic virus 1 (pcv-1) from cv "jalape+¦o m" and generated complete genomic sequences of another cryptovirus from cv "hungarian wax" designated pepper cryptic virus 2 (pcv-2). the two viruses share limited identical amino acid content in both genomic segments and appear phylogenetically closer to cryptoviruse ...201121695493
an est-ssr linkage map of raphanus sativus and comparative genomics of the brassicaceae.raphanus sativus (2n = 2x = 18) is a widely cultivated member of the family brassicaceae, for which genomic resources are available only to a limited extent in comparison to many other members of the family. to promote more genetic and genomic studies and to enhance breeding programmes of r. sativus, we have prepared genetic resources such as complementary dna libraries, expressed sequences tags (ests), simple sequence repeat (ssr) markers and a genetic linkage map. a total of 26 606 ests have b ...201121669962
evolution of phenotypic integration in brassica (brassicaceae).phenotypic integration is a necessary characteristic of living organisms that results from genetic, developmental, and functional relationships among traits. the nature of these relationships can be influenced by the environment. we examined patterns of phenotypic integration of six species of rapid cycling brassica and of raphanus sativus within a phylogenetic context. specifically, we tested the hypothesis that hybrid species show intermediate levels of integration in morphological and life-hi ...200221665666
disinfection of radish and alfalfa seeds inoculated with escherichia coli o157:h7 and salmonella by a gaseous acetic acid treatment.abstract the majority of seed sprout-related outbreaks have been associated with escherichia coli o157:h7 and salmonella. therefore, we aimed to find an effective method to inactivate these organisms on seeds before sprouting. treatment with 8.7% (v/v) acetic acid at 55°c for 2-3 h reduced the population of e. coli o157:h7 and salmonella inoculated on alfalfa (medicago sativa l.) and radish seeds (raphanus sativus l.) by more than 5.0 log cfu/g, and a longer treatment time completely eliminated ...201121651341
anthocyanin accumulation and expression of anthocyanin biosynthetic genes in radish (raphanus sativus).radish [raphanus sativus (rs)] is an important dietary vegetable in asian countries, especially china, japan, and korea. to elucidate the molecular mechanisms of anthocyanin accumulation in radish, the gene expression of enzymes directly involved in anthocyanin biosynthesis was analyzed. these genes include phenylalanine ammonia lyase (pal), cinnamate 4-hydroxylase (c4h), 4-coumarate-coa ligase (4cl), chalcone synthase (chs), chalcone isomerase (chi), flavanone 3-hydroxylase (f3h), dihydroflavon ...201121548630
[allelopathy of andrographis paniculata vegetative].andrographis paniculata at vegetative stage were analyzed for the allelopathic effect on cabbage (brassica chinensis), radis (raphanus sativus), and desmodium styracifolium, and provided the theory reference for their application of compounding planting pattern in practice.201021548354
raphasatin is a more potent inducer of the detoxification enzymes than its degradation products.the biological activity of cruciferous vegetables is hypothesized to be due to the metabolites of a class of phytochemicals called glucosinolates. the chemical properties of these metabolites, including isothiocyanates, determine the biological activity of these compounds and thus their effects on human health. the 2 primary radish (raphanus sativus l.) glucosinolates, glucoraphasatin, and glucoraphenin, were isolated using solid phase extraction followed by preparative hplc purification. in an ...201121535821
effect of 28-homobrassinolide on antioxidant defence system in raphanus sativus l. under chromium toxicity.heavy metals have emerged as major environmental contaminants due to rapid industrialization and urbanization. the genotoxic, mutagenic and carcinogenic effects of heavy metal like chromium (cr) on man, animals and plants have been documented. in plants, accumulation of heavy metals beyond critical levels generates oxidative stress. this stress is generally overcome by antioxidant defence system and stress shielding phytohormones. thus, the present study has been focused to analyze the effect of ...201121448625
bioavailability of sulforaphane from two broccoli sprout beverages: results of a short-term, cross-over clinical trial in qidong, china.one of several challenges in design of clinical chemoprevention trials is the selection of the dose, formulation, and dose schedule of the intervention agent. therefore, a cross-over clinical trial was undertaken to compare the bioavailability and tolerability of sulforaphane from two of broccoli sprout-derived beverages: one glucoraphanin-rich (grr) and the other sulforaphane-rich (sfr). sulforaphane was generated from glucoraphanin contained in grr by gut microflora or formed by treatment of g ...201121372038
revisiting the dna c-values of the genome size-standards used in plant flow cytometry to choose the "best primary standards".flow cytometry (fcm) techniques have enabled characterization of the genome size for various plant species. in order to measure the nuclear genome size of a species, reference standards with well-established dna content are necessary. however, different 2c-values have been described for the same species used as reference standard. this fact has brought about inaccurate genome measurements, making relevant the establishment of optimal dna reference standards for plant cytometric analyses. our wor ...201121318354
the complete nucleotide sequence and genome organization of fig cryptic virus, a novel bipartite dsrna virus infecting fig, widely distributed in the mediterranean basin.two double-stranded rna (dsrna) segments of a virus with a bipartite genome identified in fig (ficus carica l.) and denoted fig cryptic virus (fcv) were cloned and sequenced. viral dsrnas are 1696 bp (rna-1) and 1415 bp (rna-2) in size. rna-1 contains a single orf (1419 nt) potentially encoding a 54 kda protein and comprising the conserved amino acid motifs of the rna-dependent rna polymerase (rdrp) domain of species of the genus alphacryptovirus. its full-length amino acid sequence has the high ...201121318240
origin of the cms gene locus in rapeseed cybrid mitochondria: active and inactive recombination produces the complex cms gene region in the mitochondrial genomes of brassicaceae.cms (cytoplasmic male sterile) rapeseed is produced by asymmetrical somatic cell fusion between the brassica napus cv. westar and the raphanus sativus kosena cms line (kosena radish). the cms rapeseed contains a cms gene, orf125, which is derived from kosena radish. our sequence analyses revealed that the orf125 region in cms rapeseed originated from recombination between the orf125/orfb region and the nad1c/ccmfn1 region by way of a 63 bp repeat. a precise sequence comparison among the related ...201021317543
expression of a radish defensin in transgenic wheat confers increased resistance to fusarium graminearum and rhizoctonia cerealis.fusarium head blight (scab), primarily caused by fusarium graminearum, is a devastating disease of wheat (triticum aestivum l.) worldwide. wheat sharp eyespot, mainly caused by rhizoctonia cerealis, is one of the major diseases of wheat in china. the defensin rsafp2, a small cyteine-rich antifungal protein from radish (raphanus sativus), was shown to inhibit growth in vitro of agronomically important fungal pathogens, such as f. graminearum and r. cerealis. the rsafp2 gene was transformed into c ...201121279533
residues of bioenergy production chains as soil amendments: immediate and temporal phytotoxicity.the current shift towards bioenergy production increases streams of bioenergy rest-products (rps), which are likely to end-up as soil amendments. however, their impact on soil remains unclear. in this study we evaluated crop phytotoxicity of 15 rps from common bioenergy chains (biogas, biodiesel, bioethanol and pyrolysis). the rps were mixed into a sandy soil and the seedling root and shoot elongation of lettuce (lactuca sativa l.), radish (raphanus sativus l.), and wheat (triticum aestivum l.) ...201021256672
response of antioxidative enzymes and apoplastic bypass transport in thlaspi caerulescens and raphanus sativus to cadmium stress.a hydroponics experiment using hyperaccumulator thlaspi caerulescens (alpine pennycress) and non-specific accumulator raphanus sativus (common radish) was conducted to investigate the short-term effect of increasing cd concentrations (0, 25, 50, 75, 100 microm) on metal uptake, chlorophyll content, antioxidative enzymes, and apoplastic bypass flow. as expected, t. caerulescens generally showed better resistance to metal stress, which was reflected by higher cd accumulation within plant tissues w ...201021166344
rapid divergence of repetitive dnas in brassica relatives.centromeric, subtelomeric, and telomeric repetitive dnas were characterized in brassica species and the related raphanus sativus and arabidopsis thaliana. in general, rapid divergence of the repeats was found. the centromeric tandem satellite repeats were differentially distributed in the species studied, suggesting that centromeric repeats have diverged during the evolution of the a/c and b genome lineages. sequence analysis of centromeric repeats suggested rapid evolution. pericentromere-assoc ...201121159321
the toxicity to plants of the sewage sludges containing multiwalled carbon nanotubes.the aim of this study was to evaluate the toxicity of sewage sludges containing multiwalled carbon nanotubes (cnts) with an outer diameter <10 nm (cnt10) or 40-60 nm (cnt60) to lepidium sativum (cress), sorghum saccharatum (sorgo), solanum lycopersicon (tomato), raphanus sativus (radish) and cucumis sativus (cucumber). cnts were also incubated in sewage sludge for 7 or 31 days to determine the effect of cnt aging on sewage sludge phytotoxicity. the influence of cnts on 4 different sewage sludges ...201121145166
developing stable progenies of ×brassicoraphanus, an intergeneric allopolyploid between brassica rapa and raphanus sativus, through induced mutation using microspore culture.induced mutations were used to improve the low seed fertility of an intergeneric allopolyploid, 'baemoochae,' ×brassicoraphanus, synthesized following hybridization between brassica rapa and raphanus sativus. the mutagen n-methyl-n-nitroso-urethane (nmu) was added to microspore cultures. four lines of nine in the mi(2) generation showed very high fertility under controlled pollination. the progeny lines (mi(3)) confirmed this result under open pollination, and excellent uniformity was observed i ...201121113703
biodepollution of wastewater containing phenolic compounds from leather industry by plant peroxidases.this study deals with the use of peroxidases (poxs) from allium sativum, ipomoea batatas, raphanus sativus and sorghum bicolor to catalyze the degradation of free phenolic compounds as well as phenolic compounds contained in wastewater from leather industry. secretory plant poxs were able to catalyze the oxidation of gallic acid, ferulic acid, 4-hydroxybenzoic acid, pyrogallol and 1,4-tyrosol prepared in ethanol 2% (v:v). efficiency of peroxidase catalysis depends strongly on the chemical nature ...201120803235
certain antioxidant enzymes and lipid peroxidation of radish (raphanus sativus l.) as early warning biomarkers of soil copper exposure.copper (cu) is a major heavy metal contaminant with various anthropogenic and natural sources. recently, using biomarkers to monitor the effects of pollutants has attracted increased interest. pot culture experiments using radish (raphanus sativus l.) was performed to investigate cu phytotoxic effects on antioxidant enzymes and other early warning biomarkers of soil cu exposure. under low dose cu stress (lower than the ec10, cu concentration reducing root length by 10%), activity and isozyme exp ...201020728270
screening of dried plant seed extracts for adiponectin production activity and tumor necrosis factor-alpha inhibitory activity on 3t3-l1 adipocytes.to search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. these activities in 3t3-l1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in japan. komatsuna (brassica rapa var. perviridis), common bean (phaseolus vulgaris l.), qing geng cai (brassica rapa var. chinensis), green soybean (glycine max) ...201020717728
antidiabetic effect of raphanus sativus root juice.context: many plants have been explored scientifically and systematically and claimed to be useful for the treatment of diabetes mellitus by various research groups worldwide. the present study is a further effort in the direction of developing a novel oral antidiabetic agent of high potential with minimal or no side effects. objective: this study screened the glycemic attributes of raphanus sativus l. (brassicaceae) root juice in normal as well as sub- and mild-diabetic models. materials and me ...201120687786
phytotoxic activities of mediterranean essential oils.twelve essential oils from mediterranean aromatic plants were tested for their phytotoxic activity, at different doses, against the germination and the initial radicle growth of seeds of raphanus sativus, lactuca sativa and lepidium sativum. the essential oils were obtained from hyssopus officinalis, lavandula angustifolia, majorana hortensis, melissa officinalis, ocimum basilicum, origanum vulgare, salvia officinalis and thymus vulgaris (lamiaceae), verbena officinalis (verbenaceae), pimpinella ...201020657443
regulation of growth and antioxidant enzyme activities by 28-homobrassinolide in seedlings of raphanus sativus l. under cadmium stress.28-homobrassinolide (28-hbl), a brassinosteroid is reported to play significant role in diverse physiological processes. it induces a range of cellular and adaptive responses to a range of environmental stresses. cadmium (cd) is a non-essential metal which alters various physiological processes and generates ros, which can oxidize biological macromolecules and causes oxidative stress. this stress is generally overcome by the internal antioxidative defense system and stress shielding phytohormone ...201020653289
hexane extract of raphanus sativus l. roots inhibits cell proliferation and induces apoptosis in human cancer cells by modulating genes related to apoptotic pathway.raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. it has been used in indigenous system of medicine for the treatment of various human ailments in india. this present study evaluated the chemopreventive efficacy of different parts of r. sativus such as root, stem and leaves, extracted with solvents of varying polarity and investigated the molecular mechanism leading to growth arrest and apoptotic cell death in ...201020652750
antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (raphanus sativus var. niger, brassicaceae).the antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. the extract appeared to form a complex with fe(3+) or cu(2+). it displayed a concentration-dependant reducing power (1.16od(700 nm) at a concentration of 4mm) and scavenging effect against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals (with ic(50)=1.74 +/- 0.03 mm). it could promote the cle ...201020600520
transcriptional responses to exposure to the brassicaceous defence metabolites camalexin and allyl-isothiocyanate in the necrotrophic fungus alternaria brassicicola.summary alternaria brassicicola is the causative agent of black spot disease of brassicaceae belonging to the genera brassica and raphanus. during host infection, a. brassicicola is exposed to high levels of antimicrobial defence compounds such as indolic phytoalexins and glucosinolate breakdown products. to investigate the transcriptomic response of a. brassicicola when challenged with brassicaceous defence metabolites, suppression subtractive hybridization (ssh) was performed to generate two c ...200720507491
in-vitro free radical scavenging, antiproliferative and anti-zearalenone cytotoxic effects of 4-(methylthio)-3-butenyl isothiocyanate from tunisian raphanus sativus.the aim of this study was to investigate the antiradical and antioxidant properties of 4-(methylthio)-3-butenyl isothiocyanate (mtbitc) extracted from raphanus sativus and to assess the effects of mtbitc on tumour cell growth, cytotoxicity induced by zearalenone, an oestrogenic mycotoxin, and modulation of the expression of the genes involved in these aspects of cell behaviour.201020487203
two novel sulfur compounds from the seeds of raphanus sativus l.the seeds of raphanus sativus l., known as lai-fu-zi in traditional chinese medicine, are always roasted before clinical use for avoiding nausea. during an investigation of the chemical difference between roasted and pre-roasted products, two novel sulfur-containing compounds, which mainly existed in the pre-roasted products, were isolated. their structures and absolute configurations were established by spectroscopic and x-ray diffraction analysis.201020390752
immunotoxicity of zearalenone in balb/c mice in a high subchronic dosing study counteracted by raphanus sativus extract.radish (raphanus sativus) is a cruciferous plant, rich on flavonoids, isothiocyanates, and phenolic acids. they show anti-inflammatory and immunomodulatory activity both in vitro and in vivo. isothiocyanates and flavonoids have been reported previously to prevent low-sub-chronic dose of zearalenone (zen) causing immunotoxicity. the present study focuses on the amelioration of fusarotoxicosis in balb/c mice by feeding two concentrations of radish extract. the extract at 15 and 30 mg/kg bw, was ev ...201020205508
effect of overexpression of radish plasma membrane aquaporins on water-use efficiency, photosynthesis and growth of eucalyptus trees.eucalyptus is a diverse genus of flowering trees with more than 700 genotypic species which are mostly native to australia. we selected 19 wild provenances of eucalyptus camaldulensis grown in australia, compared their growth rate and drought tolerance and determined the protein levels of plasma membrane aquaporins (pips). there was a positive relationship between the drought tolerance and pip content. pips are divided into two subgroups, pip1 and pip2. most members of the pip2 subgroup, but not ...201020124554
characterization of acc deaminase gene in pseudomonas entomophila strain ps-pjh isolated from the rhizosphere soil.the enzyme 1-aminocyclopropane-1-carboxylate (acc) deaminase cleaves the ethylene precursor acc into alpha-ketobutyrate and ammonia. the decreased level of ethylene allows the plant to be more resistant to a wide environmental stress including plant pathogens. in the present study, we characterized the acc deaminase activity of a pseudomonas entomophila strain ps-pjh isolated from the red pepper rhizosphere region of red pepper grown at jinan, korea. the isolate produced 23.8 +/- 0.4 micromol of ...201020082369
polyphenolics profile, antioxidant and radical scavenging activity of leaves and stem of raphanus sativus l.aerial parts (leaves and stem) of raphanus sativus, which are usually discarded were found to possess potent antioxidant and radical scavenging activity, as measured by standard antioxidant assays. methanolic and acetone extracts of r. sativus leaves had total polyphenolic content of 86.16 and 78.77 mg/g dry extract, which were comparable to the traditional rich sources such as green tea and black tea. hplc identification of polyphenolics indicated the presence of catechin, protocatechuic acid, ...201020072818
effect of ambient-level gas-phase peroxides on foliar injury, growth, and net photosynthesis in japanese radish (raphanus sativus).to investigate the effects of ambient-level gas-phase peroxides concurrent with o(3) on foliar injury, photosynthesis, and biomass in herbaceous plants, we exposed japanese radish (raphanus sativus) to clean air, 50 ppb o(3), 100 ppb o(3), and 2-3 ppb peroxides + 50 ppb o(3) in outdoor chambers. compared with exposure to 100 ppb o(3), exposure to 2-3 ppb peroxides + 50 ppb o(3) induced greater damage in foliar injury, net photosynthetic rates and biomass; the pattern of foliar injury and the cau ...201020056522
qtl mapping of clubroot resistance in radish (raphanus sativus l.).a qtl analysis for clubroot resistance (cr) of radish was performed using an f(2) population derived from a crossing of a cr japanese radish and a clubroot-susceptible (cs) chinese radish. f(3) plants obtained by selfing of f(2) plants were used for the cr tests. the potted seedlings were inoculated and the symptom was evaluated 6 weeks thereafter. the mean disease indexes of the f(3) plants were used for the phenotype of the f(2). the results of two cr tests were analyzed for the presence of qt ...201020012934
effect of particle size reduction on physicochemical properties of ashgourd (benincasa hispida) and radish (raphanus sativus) fibres.the present work was aimed at studying the effect of particle size reduction on the physicochemical properties of ashgourd and radish fibre. both the fibres after extraction of juice were dehydrated and subjected to granulometry to obtain 30, 60 and 100 mesh particles. the yield was more for 60 mesh particles. the image analysis as well as microstructural analysis of particles by scanning electron microscopy showed distinctive properties. the 30 mesh particles of ashgourd and radish fibre contai ...201019961356
evolutionary trajectory of turnip mosaic virus populations adapting to a new host.little is known about how some plant viruses establish successful cross-species transmission whilst others do not; the genetic basis for adaptation is largely unknown. this study investigated the genetic changes that occurred using the progeny of an infectious clone, p35tunos, derived from the turnip mosaic virus (tumv) uk 1 isolate, which has a brassica host type, but rarely infects raphanus systemically and then only asymptomatically. the genetic trajectory leading to viral adaptation was stud ...201019906944
raphanus sativus extract prevents and ameliorates zearalenone-induced peroxidative hepatic damage in balb/c mice.raphanus sativus (radish) is a species of crucifer, which includes widely consumed vegetables, distributed in asia, africa and europe. it is a rich source of bioactive molecules including anthocyanins, glucosinolates, isothiocyanates and other flavonoids, and miscellaneous phenolic substances. we have evaluated the hepatoprotection of r. sativus extract against zearalenone, an estrogenic mycotoxin initiating hepatotoxicity in male balb/c mice.200919903381
mustard oil in "shibori daikon" a variety of japanese radish, selectively inhibits the proliferation of h-ras-transformed 3y1 cells.cruciferous vegetables and their isothiocyanates are promising foods and agents for cancer prevention. we focus here on the effects of mustard oil (smo) in a variety of the japanese radish, shibori daikon (raphanus sativus), on the proliferation of 3y1 rat fibroblasts and the h-ras-transformed derivative, hr-3y1-2. smo (1.6 microg/ml) inhibited the proliferation of hr-3y1-2, but not 3y1 after 24 h after treatment. a cell cycle analysis showed that smo induced g2/m arrest after 6 h, although this ...200919809176
involvement of s-adenosylmethionine-dependent halide/thiol methyltransferase (htmt) in methyl halide emissions from agricultural plants: isolation and characterization of an htmt-coding gene from raphanus sativus (daikon radish).biogenic emissions of methyl halides (ch3cl, ch3br and ch3i) are the major source of these compounds in the atmosphere; however, there are few reports about the halide profiles and strengths of these emissions. halide ion methyltransferase (hmt) and halide/thiol methyltransferase (htmt) enzymes concerning these emissions have been purified and characterized from several organisms including marine algae, fungi, and higher plants; however, the correlation between emission profiles of methyl halide ...200919723322
genetic and physiological determinants of streptomyces scabies pathogenicity.summary common scab is a severe disease worldwide affecting tap root crops and potato tubers. it is caused by soil-borne filamentous bacteria belonging to the genus streptomyces. streptomycetes usually are saprophytic microorganisms, but a few species have acquired the ability to infect underground plant tissues. the predominant causal agent of potato scab worldwide is streptomyces scabies. the production of phytotoxins called thaxtomins is essential for the virulence of common scab-causing agen ...200919694949
expression of a plant defensin in rice confers resistance to fungal phytopathogens.transgenic rice (oryza sativa l. cv. pusa basmati 1), overexpressing the rs-afp2 defensin gene from the raphanus sativus was generated by agrobacterium tumefaciens-mediated transformation. expression levels of rs-afp2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. it was observed that constitutive expression of rs-afp2 suppresses the growth of magnaporthe oryzae and rhizoctonia solani by 77 and 45%, respectively. no effect on plant morphology was observed in the rs-afp2 ...201019690975
raphanus sativus extract protects against zearalenone induced reproductive toxicity, oxidative stress and mutagenic alterations in male balb/c mice.zearalenone (zen) is a non-steroidal estrogenic mycotoxin produced by several species of fusarium in cereals and agricultural products. it has been implicated in several mycotoxicosis in farm animals and in humans. there is unequivocal evidence of reproductive toxicity of zen in male mice although the mechanism of action is unknown. several reports suggest that exposure to zen resulted in oxidative stress, genotoxicity and perturbation of reproductive parameters. therefore, the aim of the curren ...200919673099
the antifungal plant defensin rsafp2 from radish induces apoptosis in a metacaspase independent way in candida albicans.we show that the antifungal plant defensin raphanus sativus antifungal protein 2 (rsafp2) from radish induces apoptosis and concomitantly triggers activation of caspases or caspase-like proteases in the human pathogen candida albicans. furthermore, we demonstrate that deletion of c. albicans metacaspase 1, encoding the only reported (putative) caspase in c. albicans, significantly affects caspase activation by the apoptotic stimulus acetic acid, but not by rsafp2. to our knowledge, this is the f ...200919596007
isothiocyanate from the tunisian radish (raphanus sativus) prevents genotoxicity of zearalenone in vivo and in vitro.zearalenone (zen) is a naturally occurring contaminant of animal feed that has been implicated in several mycotoxicoses in farm livestock. recently some information has become available indicating that zen caused cancer or at least increased its prevalence, although the mechanism of action is unknown. many papers mentioned that exposure to zen results in genotoxicity and dna damage. therefore, we investigated the chemo-preventive role of 4-(methylthio)-3-butenyl isothiocyanate (mtbitc) extracted ...200919501672
a novel rf gene controlling fertility restoration of ogura male sterility by rna processing of orf138 found in japanese wild radish and its sts markers.to reveal the molecular and genetic mechanism of fertility restoration in ogura male sterility in japanese wild radish (raphanus sativus var. hortensis f. raphanistroides), we investigated fertility restoration of a plant that lacks the dominant type of orf687, a previously identified fertility restorer gene. a total of 100 f2 plants were made from the cross between a male-sterile strain with the ogura cytoplasm, 'ms-gensuke', and a japanese wild radish plant. segregation of pollen fertility in ...200919483769
the design, synthesis and anticancer activity of new nitrogen mustard derivatives of natural indole phytoalexin 1-methoxyspirobrassinol.nitrogen mustards cis-1-methoxy-2-deoxy-2-[n,n-bis(2 -chloroethyl)amino]spirobrassinol (4) and trans-1-methoxy-2-deoxy-2-[n,n-bis(2 -chloroethyl)amino]spirobrassinol (5) derived from 1-methoxyspirobrassinol, an indole phytoalexin produced by the japanese radish raphanus sativus var. hortensis were designed as prospective dual-action compounds with dna-alkylating effect and glutathione-depleting effects that may sensitize cancer cells to alkylating agents. both new compounds demonstrated cytostat ...200919473057
the insecticidal activity of four medicinal plants against the blowfly lucilia sericata (diptera: calliphoridae).the larvae of lucilia sericata induce myiasis and transmit mycobacterial infections to humans and animals. consequently, the blowfly should be controlled for human welfare and economic reasons.200919416380
combined mri-pet dissects dynamic changes in plant structures and functions.unravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. studying allocation under close to natural conditions requires non-invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. by combining magnetic resonance imaging (mri) and positron emission tomography (pet), we investigated three contrasting root/shoot systems growing in sand or soil, with respect t ...200919392708
physical localization and genetic mapping of the fertility restoration gene rfo in canola (brassica napus l.).the ogu cytoplasm for male sterility and its fertility restorer gene rfo in canola (brassica napus l.) were originally introgressed from radish (raphanus sativus l.) and have been widely used for canola hybrid production and breeding. the objective of this study was to determine the physical location of the rfo locus in the canola genome using fluorescence in situ hybridization and genetic mapping. for physical localization of the rfo gene, two bacterial artificial chromosome (bac) clones, g62 a ...200919370095
synthesis and structure--activity relationships of substituted cinnamic acids and amide analogues: a new class of herbicides.in the present investigation, substituted cinnamic acids (3-hydroxy, 4-hydroxy, 2-nitro, 3-nitro, 4-nitro, 3-chloro, and 4-methoxy) and their amide analogues with four different types of substituted anilines have been synthesized. the synthesized compounds have been screened for their germination inhibition activity on radish (raphanus sativus l. var. japanese white) seeds at 50, 100, and 200 ppm concentrations, and the activity was compared with standard herbicide, metribuzin formulation (senco ...200919368353
Refresh
firstprev
12345
nextlast
Displaying items 101 - 200 of 407