Publications

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thermophilic biodesulfurization of various heterocyclic sulfur compounds and crude straight-run light gas oil fraction by a newly isolated strain mycobacterium phlei wu-0103.various heterocyclic sulfur compounds such as naphtho[2,1-b]thiophene (nth) and benzo[b]thiophene (bth) derivatives can be detected in diesel oil, in addition to dibenzothiophene (dbt) derivatives. mycobacterium phlei wu-0103 was newly isolated as a bacterial strain capable of growing in a medium with nth as the sulfur source at 50 degrees c. m. phlei wu-0103 could degrade various heterocyclic sulfur compounds, not only nth and its derivatives but also dbt, bth, and their derivatives at 45 degre ...200515702256
evaluation of bovine cutaneous delayed-type hypersensitivity (dth) to various test antigens and a mitogen using several adjuvants.the bacillus calmette guerin (bcg)-induced/purified protein derivative (ppd)-elicited tuberculin skin test is a reliable measure of cell-mediated immune response (cmir), specifically delayed-type hypersensitivity (dth); however, its use in livestock may confound diagnosis of mycobacterium tuberculosis. therefore, various alternative antigen/adjuvant combinations were evaluated as inducers of dth that were compared to the bcg/ppd test system with the purpose of finding a skin dth protocol that do ...200515661330
identification of a cation-specific channel (tipa) in the cell wall of the gram-positive mycolata tsukamurella inchonensis: the gene of the channel-forming protein is identical to mspa of mycobacterium smegmatis and mppa of mycobacterium phlei.detergent extracts of whole cells of the gram-positive bacterium tsukamurella inchonensis atcc 700082, which belongs to the mycolata, were studied for the presence of ion-permeable channels using lipid bilayer experiments. one channel with a conductance of about 4.5 ns in 1 m kcl was identified in the extracts. the channel-forming protein was purified to homogeneity by preparative sds-page. the protein responsible for channel-forming activity had an apparent molecular mass of about 33 kda as jud ...200415533305
the distinction of licheniformin from subtilin by cross-reactions with antibiotic-resistant strains of mycobacterium phlei. 195015415580
identification and functional analysis of the genes encoding dibenzothiophene-desulfurizing enzymes from thermophilic bacteria.thermophilic bacteria bacillus subtilis wu-s2b and mycobacterium phlei wu-f1 desulfurize dibenzothiophene (dbt) and alkylated dbts through specific cleavage of the carbon-sulfur bonds over a temperature range up to 52 degrees c. in order to identify and functionally analyze the dbt-desulfurization genes, the gene cluster containing bdsa, bdsb, and bdsc was cloned from b. subtilis wu-s2b. the nucleotide and amino acid sequences of bdsabc show homologies to those of the other known dbt-desulfuriza ...200415221222
increased mitogen-activated protein kinase activity and tnf-alpha production associated with mycobacterium smegmatis- but not mycobacterium avium-infected macrophages requires prolonged stimulation of the calmodulin/calmodulin kinase and cyclic amp/protein kinase a pathways.previous studies have shown the mitogen-activated protein kinases (mapks) to be activated in macrophages upon infection with mycobacterium, and that expression of tnf-alpha and inducible no synthase by infected macrophages was dependent on mapk activation. additional analysis demonstrated a diminished activation of p38 and extracellular signal-regulated kinase (erk)1/2 in macrophages infected with pathogenic strains of mycobacterium avium compared with infections with the fast-growing, nonpathog ...200415100302
cloning and expression of mycobacterial glutamine synthetase gene in escherichia coli.extracellular glutamine synthetase (gs) is one of the prominent proteins secreted by pathogenic mycobacteria such as mycobacterium tuberculosis and mycobacterium bovis. non-pathogenic species like mycobacterium smegmatis and mycobacterium phlei do not secrete this protein. gs has been proposed to play an indispensable role in intracellular survival of pathogenic mycobacteria. in this study, the structural gene for extracellular glutamine synthetase of m. tuberculosis was pcr amplified and expres ...200415063805
the activity of milk leukocytes in response to a water-soluble fraction of mycobacterium phlei in bovine subclinical mastitis.the effect of a water-soluble fraction (wsf) of a non-pathogenic strain of mycobacterium phlei was studied in bovine subclinical mastitis (scm) by measuring the myeloperoxidase and acid phosphatase enzyme levels in the milk leukocytes. forty-five cows were divided into three equal groups. group i, consisting of 15 healthy cows, served as the control, whereas groups ii and iii each contained 15 cows with subclinical mastitis on the basis of a positive reaction in the california mastitis test (cmt ...200414989362
further use of diphenylamine for the study of carotenoid biosynthesis in mycobacterium phlei. 195214955505
[an amino acid of the phosphatide of mycobacterium phlei]. 195214953939
[studies and research on mycobacteria. vii. mycobacterium phlei (lehmann et neumann 1899 pro parte)]. 195114930275
endometrial il-1beta, il-6 and tnf-alpha, mrna expression in mares resistant or susceptible to post-breeding endometritis. effects of estrous cycle, artificial insemination and immunomodulation.endometrial mrna expression of the pro-inflammatory cytokines interleukin-1beta (il-1beta), interleukin-6 (il-6), and tumor necrosis factor alpha (tnf-alpha) was assessed in mares resistant (rm) or susceptible (sm) to persistent post-breeding endometritis (ppbe). eight rm and eight sm, were selected based on reproductive records and functional tests out of a herd of 2,000 light cross-type mares. three experiments were done to study transcription patterns in (i) basal conditions; (ii) after artif ...200314522132
the biosynthesis of oleic and 10-methylstearic acids in mycobacterium phlei. 196214463993
endogenous respiration of mycobacterium phlei at various temperatures. 196214462642
aberrant forms of mycobacterium phlei produced by streptomycin and their multiplication on streptomycin-free media. 196214452730
[methods for measurement of reproduction respiration of mycobacterium phlei]. 196014408355
subcellular distribution of a biologically active naphthoquinone mycobacterium phlei. 196014404646
[effect of acetic acid on formation of oxalic acid in metabolism of mycobacterium phlei]. 195414376088
[caloric and material balance of mycobacterium phlei]. 195414376084
[citric acid in mycobacterium phlei cultures]. 195414376083
[effect of a complex of iron with ethylenediaminotetra-acetic acid on respiration of mycobacterium phlei]. 195414376082
the structure of mycobactin p, a growth factor for mycobacterium johnei, and the significance of its iron complex.1. the growth factor from mycobacterium phlei is now named mycobactin p to distinguish it from related but chemically distinct growth factors from other species of mycobacteria. it is shown to comprise four closely similar chemical entities differing only in the fatty side chain. 2. the structure of the main component (85%) of mycobactin p has been established and the absolute configuration of all asymmetric centres determined. 3. ferric mycobactin p has been crystallized; its structure is discu ...196514342223
biosynthesis of a vitamin k2 by cell-free extracts of mycobacterium phlei. 196514332442
the identity and mechanism of formation of free radicals in electron transport particles from mycobacterium phlei. 196514285525
the appearance and general properties of free radicals in electron transport particles from mycobacterium phlei. 196514285524
oxidative phosphorylation in fractionated bacterial systems. xiv. respiratory chains of mycobacterium phlei. 196414247683
cell walls of three strains of mycobacteria (mycobacterium phlei, mycobacterium fortuitum and mycobacterium kansasii). preparation, analysis and digestion by lysozymes of different origins. 196414236706
[gas chromatographic study of the fatty acids of mycobacterium phlei (abstract)]. 196414228917
inorganic polyphosphate glucokinase of mycobacterium phlei. 196414212975
structural studies on the myo-inositol mannodides from the glycolipids of mycobacterium tuberculosis and mycobacterium phlei. 196414193828
cofactor requirements for the formation of delta-9-unsaturated fatty acids in mycobacterium phlei. 196414167617
the effect of cholesterol on the growth of mycobacterium tuberculosis, bcg and mycobacterium phlei. 196314093400
growth of pathogenic leptospirae by replacing rabbit serum with mycobacterium phlei or mycobacterium smegmatis. 196314080790
role of an unidentified factor involved in electron transport in mycobacterium phlei. 196314077502
[biochemical and immunological studies on desoxyribonucleic acids from mycobacterium phlei and the thymus gland]. 196314058035
absorption of the antibacterial serum factor by staphylococci.yotis, william w. (loyola university, chicago, ill.). absorption of the antibacterial serum factor by staphylococci. j. bacteriol. 85:911-917. 1963.-the absorption of antibacterial serum factor by eight bacterial species and one yeast showed good correlation with the sensitivity of the organisms to the serum factor. the serum factor was removed from aqueous solution by coagulase-positive and coagulase-negative strains of staphylococci and bacillus subtilis, and the oxygen consumption of the bact ...196314044962
[transamination reactions in mycobacterium phlei]. 196014038915
effect of mycobacterium phlei upon reticuloendothelial system of mice of different ages. 196314027679
bimodal stimulation of the reticuloendothelial system by mycobacterium phlei. 196314027678
the isolation and identification of a naphthoquinone from electron-transport particles of mycobacterium phlei. 196313999394
relationship of the hexose monophosphate shunt to the endogenous metabolism of cell-free extracts of mycobacterium phlei.sutton, w. b. (the lilly research laboratories, indianapolis, ind.). relationship of the hexose monophosphate shunt to the endogenous metabolism of cell-free extracts of mycobacterium phlei. j. bacteriol. 85:476-484. 1963.-the endogenous reduction of 2,6-dichlorophenol-indophenol (dpip) by cell-free extracts of mycobacterium phlei has been linked to the presence of glucose-6-phosphate (g-6-p) dehydrogenase functioning in connection with a reduced triphosphopyridine nucleotide (tpn)-dpip reductas ...196313979428
cofactor requirements for fatty acid desaturation in mycobacterium phlei. 196213960214
characterization of vitamin k9(h) from mycobacterium phlei. 196313946232
[studies on the antigenic structure of mycobacteria. i. on the antigenic structure of the human type h 2 and nakano strains, the bovine type bcg and ravenel strains, the avian type a. f. strain, the grass bacillus, and mycobacterium phlei]. 196213918370
[study of the effects produced by ultraviolet light on bacteria. ii. changes in the physiological behavior of mycobacterium phlei]. 196113875400
[study of the effects produced by ultraviolet light on bacteria. v. change in the metabolism of nucleic acids in mycobacterium phlei]. 196113875399
studies on the toxic substances isolated from mycobacteria. ii. toxic glycolipids of mycobacterium smegmatis, mycobacterium phlei and atypical mycobacteria strain no. 6 and no. 22. 196213863583
the new synthetic 6-chromanyl phosphate of vitamin k1(20) and its behavior in an enzymatic system from mycobacterium phlei. 196213862733
[on the effect of enzyme inhibitors on isoniazid-sensitive and resistant cells of mycobacterium phlei]. 196013854036
[on differences in oxygen consumption of proliferating inh-sensitive and inh-resistant cells of mycobacterium phlei in the oxidative assimilation of various substrates]. 195913853059
[on the presence of acylamidases in mycobacteria. part iv. the differentiation of rapidly growing mycobacteria of the species mycobacterium smegmatis. mycobacterium phlei. mycobacterium fortuitum and mycobacterium thamnopheos by demonstration of their different acylamidase metabolism ability]. 196013849090
pyridine nucleotide coenzymes of mycobacterium phlei. 196013843316
[electron microscopic studies on mycobacterium phlei. (structure and formation of metachromatic granula)]. 196013818082
[development of resistance to penicillin, streptomycin and aureomycin in mycobacterium phlei and escherichia coli]. 196013775755
[studies on polyphosphate metabolism and the formation of metachromatic granula in mycobacterium phlei]. 196013724377
is mycobacterium phlei glutamotransferase endowed with glutamine synthetase activity? 196113710925
taxonomically significant color reactions of brevibacterium linens.grecz, nicholas (university of chicago, chicago, ill.), and gail m. dack. taxonomically significant color reactions of brevibacterium linens. j. bacteriol. 82:241-246. 1961.-brevibacterium linens was observed to give characteristic color reactions with certain bases and acids. an intensive carmine-red color appeared immediately after addition of a drop of 5 n sodium hydroxide, 5 n potassium hydroxide, and saturated barium hydroxide. a light carmine-red was given by lithium hydroxide, and a light ...196113708147
polysaccharides of mycobacterium phlei. 196113704655
mycobacterium tuberculosis h37rv grown in vivo: nature of the inhibitor of lactic dehydrogenase of mycobacterium phlei. 196113684572
conversion of carotenoids to oxycarotenoids by mycobacterium phlei. 195913641189
[transamination of basic amino acids in mycobacterium phlei]. 195713532884
[aspartic acid-glutamic acid transaminase in mycobacterium phlei]. 195713532790
pathways involved in cytochrome c reduction in mycobacterium phlei. 195713471605
growth characteristics in hela cells of the rapidly growing acid fast bacteria, mycobacterium fortuitum, mycobacterium phlei, and mycobacterium smegmatis. 195713449037
mechanism of action and crystallization of lactic oxidative decarboxylase from mycobacterium phlei. 195713428772
[chemical studies of mycobacterium phlei]. 195613412938
[behavior of hemoproteins in the presence of acid-fast bacilli. ii. effect of oxy- and methemoglobin on respiration of mycobacterium phlei and mycobacterium tuberculosis strain c]. 195613394225
[morphology of mycobacterium phlei in successive phases of development, observed in microculture slides]. 195613388189
inhibition of nicotinamidase activity in cell-free extracts of mycobacterium phlei by 3-acetylpyridine. 195613341963
[pentose cycle in carbohydrates in mycobacterium phlei]. 195513338984
studies on the interaction between phagocytes and tubercle bacilli. ii. the action of phagocytes upon c14-labelled tubercle bacilli.tubercle bacilli labelled with c(14) were prepared by growth on radioactive substrates such as glycerol, co(2), and acetate. these organisms were exposed in vitro to leucocytes (mostly polymorphonuclear leucocytes) from peritoneal exudates of guinea pigs. the respiration of the leucocytes and of the bacilli, alone and together, was followed by determining oxygen uptake and c(14)o(2) production. when heat-killed labelled tubercle bacilli were exposed to leucocytes there was little or no degradati ...195613332185
determination of l-glutamic acid by use of l-glutamic acid decarboxylase from mycobacterium phlei. 195613322939
inhibition of l-asparaginase in extracts of mycobacterium phlei by d-asparagine. 195613321923
serologic investigations of mycobacterial antigens. ii. specificities of antigens obtained by successive aqueous extraction of bcg and mycobacterium phlei. 195613302687
serologic investigations of mycobacterial antigens. i. the specificities of aqueous and saline extracts of bcg, mycobacterium phlei, and h37rv. 195613302686
[synthesis and utilization of citric acid by mycobacterium phlei]. 195513301218
animal studies on effects of bcg, h37ra and mycobacterium phlei in tuberculosis immunization. 195613299365
studies in carotenogenesis. 17. the carotenoids produced by different strains of mycobacterium phlei. 195613293182
the electron-dense bodies of mycobacterium phlei. 195513278479
[asparagino-alpha-ketoglutaric (asparagino-glutamic) transamination in mycobacterium phlei]. 195413273216
sulfhydryl and prosthetic groups of lactic oxidative decarboxylase from mycobacterium phlei. 195513271350
experimental studies of vaccination, allergy, and immunity in tuberculosis. vi. comparative effects of bcg, h37ra, mycobacterium phlei and a wax-protein fraction of human tubercle bacilli. 195513268417
[intermediary metabolism of mycobacteria: degradation of various carbohydrates by mycobacterium tuberculosis and mycobacterium phlei]. 195413219903
isolation and properties of a lactic oxidative decarboxylase from mycobacterium phlei. 195413201592
[hexosophosphoric ethers in acetone preparation of mycobacterium tuberculosis h37ra and in mycobacterium phlei]. 195413198990
[the isolation and chemical constitution of the mycolic acids from mycobacterium phlei and mycobacterium smegmatis]. 195413172243
[studies on labeled mycobacteria. i. mycobacterium phlei and radiophosphorus; technic of obtaining and rate of utilization of exogenous phosphorus]. 195313124994
mycobactin, a growth factor for mycobacterium johnei. i. isolation from mycobacterium phlei. 195313115341
rapidly growing, acid fast bacteria. i. species' descriptions of mycobacterium phlei lehmann and neumann and mycobacterium smegmatis (trevisan) lehmann and neumann. 195313069464
[ribonucleic acid of mycobacterium phlei]. 195313067311
growth inhibitory action of copper for mycobacterium phlei. 195313034708
nutrition of mycobacterium phlei. ii. studies on the nitrogen requirement. 195212999696
nutrition of mycobacterium phlei. i. requirements for rapid growth. 195212999695
citrate formation in mycobacterium phlei. 195212999691
[isolation and chemical constitution of two phleimycolic acids of mycobacterium phlei]. 195212997497
[intermediate metabolism of mycobacteria. i. the cytochromes of human (h37ra), bovine (bcg), and avian (a17) strains of mycobacterium tuberculosis and mycobacterium phlei]. 195212978698
antibacterial activity of berry fruits used for culinary purposes.increasing interest in the health benefits of various culinary berries has led to investigation of their antibacterial activity. commercial raspberry, blackcurrant, cranberry, and blackberry cordials (100% fruit) as well as fresh berries were assessed for their ability to inhibit the growth of various bacteria and the yeast candida albicans. three of the six raspberry cordials and the blackcurrant cordial inhibited all 12 bacteria and c. albicans at dilutions of 1:5. bacteria showed varying susc ...200312804021
thermophilic biodesulfurization of hydrodesulfurized light gas oils by mycobacterium phlei wu-f1.recalcitrant organosulfur compounds such as dibenzothiophene (dbt) derivatives in light gas oil (lgo) cannot be removed by conventional hydrodesulfurization (hds) treatment using metallic catalysts. the thermophilic dbt-desulfurizing bacterium mycobacterium phlei wu-f1 grew in a medium with hydrodesulfurized lgo as the sole source of sulfur, and exhibited high desulfurizing ability toward lgo between 30 and 50 degrees c. when wu-f1 was cultivated at 45 degrees c with b-lgo (390 ppm s), f-lgo (12 ...200312694922
development of short non-cpg phosphodiester oligonucleotides as immune stimulatory agents.we have previously reported that dna isolated from mycobacterium phlei (m. phlei) stimulates the synthesis of cytokines by monocytes and macrophages independently of the presence of unmethylated cpg motifs. oligonucleotides as small as five to six bases isolated from m. phlei dna have been found to induce cytokine synthesis. in the present study, we have investigated the potential for such cpg-lacking dna to act as an immune stimulant. a series of six base length phosphodiester oligonucleotides ...200312547612
growth of mycobacteria on carbon monoxide and methanol.several mycobacterial strains, such as mycobacterium flavescens, mycobacterium gastri, mycobacterium neoaurum, mycobacterium parafortuitum, mycobacterium peregrinum, mycobacterium phlei, mycobacterium smegmatis, mycobacterium tuberculosis, and mycobacterium vaccae, were found to grow on carbon monoxide (co) as the sole source of carbon and energy. these bacteria, except for m. tuberculosis, also utilized methanol as the sole carbon and energy source. a co dehydrogenase (co-dh) assay, staining by ...200312486050
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