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pyelonephritis in cows: 15 cases (1982-1986).medical records of 15 adult cows with a final diagnosis of pyelonephritis were evaluated retrospectively. only 3 cows had obvious clinical signs that indicated urinary tract disease. physical examination, including rectal and vaginal examinations, and urine chemical reagent strip screening detected abnormalities in all 15 cows and allowed subsequent confirmation by culture results of urine samples obtained using a catheter. bacteriologic cultures of urine revealed corynebacterium renale infectio ...19892649473
isolation and characterization of monoclonal antibodies against pili of corynebacterium renale and corynebacterium pilosum.five monoclonal antibodies against pili of corynebacterium renale 115 p+ (piliated clone) and two monoclonal antibodies against pili of c. pilosum 92 p+ (piliated clone) were produced. these antibodies bound to pili of the homologous strain in in enzyme-linked immunosorbent assay (elisa) and agglutinated p+ but not p- (non-piliated clone) of each homologous strain. the five monoclonal antibodies against c. renale 115 p+ pili were divided into 2 groups, comprising 16/5, 160/1 and 32/6 and 13/4 an ...19872433832
ph regulation of urease levels in streptococcus salivarius.potential mechanisms for regulation of urease levels in streptococcus salivarius were examined, including: induction by urea, nitrogen or carbon source repression, and effects of ph and co2 (because co2 enrichment enhanced urease detection on urea agar plates). regulation by either ph or co2 was confirmed by comparison of the urease accumulation pattern during anaerobic growth under co2 with that under n2. under co2, there was an initial buffering plateau at ph 6.2 and a rate of streptococcus sa ...19902110582
cloning and expression of a pili gene of corynebacterium renale in escherichia coli.a plasmid gene library of corynebacterium renale piliated strain no. 109p+ was prepared in escherichia coli in order to study the chemical structure of the pili of c. renale. of 3,000 recombinant clones tested, 5 reacted with anti-pili anti-serum. the gene products of these clones reacted with anti-pili monoclonal antibodies 8/4, 5/2 and b20/3 but lacked the reactivity with 13/4. sds-page analysis revealed that the expressed protein had a molecular mass of 48 kilodalton and deletion analysis sho ...19901968989
isolation of corynebacterium renale from slaughtered cattle at the zaria abattoir in nigeria.forty urinary bladders were collected from apparently healthy cattle slaughtered at the zaria abattoir. twenty-four (60%) were from male animals and 16 (40%) from females. urine samples were obtained and cultured for corynebacterium renale. four (16.7%) of the samples from males and 1 (6.3%) from females were positive. the difference in infection between the sexes was statistically significant (p less than 0.001).19911818361
normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta).the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ...19911803013
[urinary tract infection caused by corynebacterium sp.--a case report and an experimental study].we repose of chronic cystitis associated with alkali urine, struvite stones and a subsequent vesicorectal fistula caused by corynebacterium sp, probably corynebacterium group d2. we also studied in vitro and in vivo stone formation caused by corynebacterium renale isolated clinically. c. renale inoculated into normal human urine increased urine ph and formed struvite crystals after a 24-hr incubation. bladder stones were also formed in rats experimentally infected by c. renale as well as p. mira ...19921529815
effect of hydroxamic acids on growth and urease activity in corynebacterium renale.studies were conducted on the effect of four different hydroxamic acids (ha), hydroxyurea, acetohydroxamic acid, p-flurobenzoylhydroxamic acid and sorbylhydroxamic acid, on the growth and urease activity of corynebacterium renale. the addition of each of these ha, at concentrations ranging form 10(-3) to 10(-5) m, to medium containing urea as the sole nitrogen source resulted in a lengthened lag period of growth the extent of which depended upon the concentration of each ha tested as well as the ...19761260545
[attachment of corynebacterium renale to animal cells by fimbria]. 19751240309
[classification of corynebacterium renale]. 19751240301
efficacy of antibiotic treatment in cows affected with cystitis and those affected with pyelonephritis due to corynebacterium renale. 19751238865
some aspects of the immunological response in cattle infected with corynebacterium renale. identification of a new serotype of c. renale, type iv. 19751199528
attachment of corynebacterium renale to tissue culture cells by the pili.one or more cells of corynebacterium renale strains (serologic types, i, ii and iii), which possessed numerous pili, frequently were attached to bhk-21 cells, primary dog kidney cells, and primary rabbit kidney cells. the percentage of the cultured cells to which c renal cells were attached was about 70%. the percentage was less with cells of c renale possessing fewer pili, around 30%. after c renale was treated with the homologous anti-pili serum, the percentage of bhk-21 cells to which bacteri ...19751190607
distribution of corynebacterium renale among healthy bulls with special reference to inhabitation of type iii in the prepuce.distribution of corynebacterium renale among apparently healthy bulls reared in hokkaido was investigated. the organism was detected from 46 (39.3%) of 117 specimens of preputial cavity washing and from 60 (51.7%) of 116 specimens of semen. the isolates studied in this survey belonged to type iii, except a few which belonged to type ii. no type i strain was isolated from any bull. c. renale type iii was isolated from the prepuce in six of seven bulls slaughtered and from urethra in three, but no ...19751186911
experimental model of corynebacterium renale pyelonephritis produced in mice.corynebacterium renale type i (strain 115), 1.7 x 10(7) to 4.5 x 10(7) organisms, introduced intravenously into mice disappeared from the blood less than 24 h after inoculation and did not produce pyelonephritis. the same strain, 1 x 10(7) to 5 x 10(7) organisms, inoculated into the urinary bladder of mice was not recovered from the blood in any of the mice, but caused pyelonephritis accompanied by ureteritis and cystitis in 16 of 21 (76%) mice. pyelonephritis and cystitis in mice were histopath ...1977873610
influence of iron on corynebacterium renale-induced pyelonephritis in a rat experimental model.growth of corynebacterium renale in vitro on low-iron medium (1.34 micron) was only slightly less than that on high-iron media (7.16 and 9.85 micron). however, studies on c. renale-induced pyelonephritis using the rat as an experimental model revealed that c. renale cultivated in high-iron media was capable of producing pyelonephritis, but when grown on low-iron medium, these bacteria were noninfective. this apparent avirulence of the bacteria cultivated on low levels of iron could be reversed b ...1978689735
pili-mediated attachment of corynebacterium renale to mucous membrane of urinary bladder of mice.corynebacterium renal strain 115 with numerous pili became attached in vivo to the mucous membrane of the urinary bladder of mice 10 to 30 times more frequently than did that of c renale american type culture collection 19412, which showed few pili. antipili serum-treated c renale strain 115 was not recovered from the membrane in as large amounts as was untreated bacteria. antisomatic serum-treated strain 115, on the other hand, was recovered from the membrane in amounts similar to untreated bac ...1978629438
minimal nitrogen requirements of corynebacterium renal strains.corynebacterium renale strain 10849 was grown in a chemically defined medium containing glucosamine, ammonium sulfate, and 5 amino acids as possible nitrogen sources. although glucosamine was slightly stimulatory, its omission from the medium had a minimal effect on growth, and washed cells introduced into glucosamine-free medium grew readily through 10 serial transfers, demonstrating that this compound was not required for growth. individual omissions of isoleucine, valine, methionine, and glut ...1978629434
no effect of cyclophosphamide on ascending of corynebacterium renale from urinary bladder into kidneys in mice. 1977599754
cell wall sugars and amino acids of three immunological types of corynebacterium renale. 1977599753
experimental rat model for corynebacterium renale-induced pyelonephritis.the laboratory rat was able to serve as a model for ascending pyelonephritis after implantation of a zinc disk coated with corynebacterium renale into the urinary bladder because it satisfied three different criteria for infection. the production of an alkaline urine and the presence of significant numbers of c. renale in the kidneys, as well as distinct pyelonephritic lesions as revealed by histological examination, were observed in all rats infected with c. renale. control rats that harbored s ...1977591068
[a trial on nutritionally forced mating of conventional glutamate-producing bacteria].by nutritionally forced mating, we tried to find the sex factor in glutamate producing bacteria with some auxtrophic mutants of corynebacterium glutamicum, micrococcus glutamicus and brevibacterium divaricatum. we failed to find true mates in this trial, even when the cells cured by acridine orange were used to mate with non-cured ones. there were some positive growth when two densed cell suspensions were dropped on the minimum medium, but after subcultured on the minimum medium no growth was fo ...1979583276
antibody formation in corynebacterium renale-induced experimental pyelonephritis in the rat.the immune defense system of the kidney was studied by inducing ascending pyelonephritis in rats with corynebacterium renale. with the fluorescent antibody technique, c renale organisms were observed in the renal pelvis, but were not coated with antibody until they reached the medulla. histopathologic evaluation of renal tissues collected serially after inoculation confirmed the presence of infection in the medulla when antibody coating occurred. serum anti-c renale antibody concentrations incre ...1979525896
ulcerative posthitis in bulls in uruguay.ulcerative posthitis in bulls in uruguay is described; 1096 bulls in 17 different establishments were examined. the incidence of bulls grazing on improved pasture was 32.9% in those under one year and 80.8% in animals of 18 to 36 months of age. bulls grazing on natural pasture showed an incidence of 63.5% in adult animals irrespective of age. histologic characteristics of the preputial lesions were acanthosis, parakeratosis and hyperkeratosis followed by invasion of leukocytes in the epithelium ...1979455992
antibody coated bacteria in urine sediment from cattle infected with corynebacterium renale.using the fluorescent antibody test, the presence of antibody-coated bacteria in 10 out of 17 urine sediment samples from cattle infected with corynebacterium renale is described. these antibodies were mainly of the immunoglobulin class igg, and to a lesser extent iga. this finding is characteristic for infections of the upper urinary tract (pyelonephritis). in seven samples no antibody coating of the bacterial surface was detected. in these cases an infection of the lower urinary tract (cystiti ...1979390848
specific immunofluorescence of corynebacterium renale. 1979367528
a numerical taxonomic study of the strains of three types of corynebacterium renale.numerical analysis has been carried out on 100 features of 55 strains of corynebacterium renale. three phena were formed, which respectively corresponded to three immunological types of c. renale.1975238729
influence of acetohydroxamic acid on experimental corynebacterium renale pyelonephritis.the role of corynebacterium renale urease in the establishment of pyelonephritis was studied by the oral administration of acetohydroxamic acid (aha), a urease inhibitor, to experimentally infected rats. the bacteria were introduced by surgical insertion of a zinc disc containing 1 x 10(6) colony-forming units of c-renale into the urinary bladder whereas sterile discs were implanted in the bladders of the control animals. daily administration of aha via the drinking water did not halt the develo ...197721738
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