Publications

molecular profiling of lactobacillus, streptococcus, and bifidobacterium species in feces of active racehorses.	diversity and compositions of the lactobacillus, streptococcus, and bifidobacterium group in the feces of six healthy, actively racing horses (thoroughbreds) were analyzed by using pcr-denaturing gradient gel electrophoresis (dgge) and real-time pcr with primer sets specific for each group. pcr-dgge analysis of the feces showed that lactobacillus equi, lactobacillus johnsonii, a phylogenetic relative of lactobacillus salivarius, a phylogenetic relative of lactobacillus gastricus, and weissella c ...	2007	17726300
lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin.	to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development.	2007	17650194
characterization of italian cheeses ripened under nonconventional conditions.	four italian cheeses (casciotta di urbino, barricato san martino, vento d'estate, and ubriaco di raboso) nonconventionally ripened under different plant materials (walnut leaves, herbs, hay, and wine by-products, respectively) were compared for compositional, microbiological, biochemical, and volatile profile characteristics. mean values for gross composition were rather similar. because primary starters were not used for manufacture, the endogenous lactic acid bacteria were mainly present (7.0 ...	2007	17517708
an irreversible and kinetically controlled process: thermal induced denaturation of l-2-hydroxyisocaproate dehydrogenase from lactobacillus confusus.	the thermal denaturation of lactobacillus confusus l-2-hydroxyisocaproate dehydrogenase (l-hicdh) has been studied by differential scanning calorimetry (dsc). the stability of this enzyme has been investigated at different ph conditions. the results of this study indicate that the thermal denaturation of this enzyme is irreversible and the t(m) is dependent on the scan-rate, which suggests that the denaturation process of l-hicdh is kinetically determined. the heat capacity function of l-hicdh s ...	2007	17205397
severe infective endocarditis of native valves caused by weissella confusa detected incidentally on echocardiography.	weissella confusa is gram-positive, catalase-negative, short rod or coccobacillus that has been isolated from a wide variety of habitats. we describe a rare, severe infective endocarditis of native valves caused by w. confusa that was detected incidentally on echocardiography. the patient recovered completely with valvular replacement plus antibiotic therapy.	2007	17052757
antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms.	non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ...	2007	17049799
alteration of the canine small-intestinal lactic acid bacterium microbiota by feeding of potential probiotics.	five potentially probiotic canine fecal lactic acid bacterium (lab) strains, lactobacillus fermentum lab8, lactobacillus salivarius lab9, weissella confusa lab10, lactobacillus rhamnosus lab11, and lactobacillus mucosae lab12, were fed to five permanently fistulated beagles for 7 days. the survival of the strains and their potential effects on the indigenous intestinal lab microbiota were monitored for 17 days. denaturing gradient gel electrophoresis (dgge) demonstrated that the five fed lab str ...	2006	17021203
defined multi-species semi-liquid ready-to-use sourdough starter.	this paper aimed at setting up a protocol for the manufacture of a defined multi-species semi-liquid ready-to-use sourdough starter consisting of lactic acid bacteria (lab) selected on the basis of their capacity of rapid acidification and adaptation to several technological factors. preliminarily, 56 strains of sourdough lab were screened based on the acidification kinetics during sourdough fermentation. the influence of temperature (25-37 degrees c), nacl (2%, w/w, of wheat flour), initial cel ...	2007	16943090
lactic acid bacteria isolated from canine faeces.	lactic acid bacteria (lab) were isolated and sequenced from the faeces of healthy dogs. five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine.	2006	16834600
isolation and characterization of microorganisms associated with the traditional sorghum fermentation for production of sudanese kisra.	sorghum flour obtained from sudan was mixed with water in a 1:2 (wt/vol) ratio and fermented at 30 degrees c for 24 h. the bacterial populations increased with fermentation time and reached a plateau at approximately 18 h. at the end of 24 h, sorghum batter ph had dropped from 5.95 to 3.95 and the batter had a lactic acid content of 0.80%. the microbial population during the 24 h of fermentation consisted of bacteria (pediococcus pentosaceus, lactobacillus confusus, lactobacillus brevis, lactoba ...	1991	16348551
phenotypic identification and technological properties of lactic acid bacteria isolated from traditionally processed fish products of the eastern himalayas.	sukako maacha, gnuchi, sidra and sukuti are traditional smoked and sun-dried fish products of the eastern himalayan regions of nepal and india. a total of 40 samples of sukako maacha (14), gnuchi (6), sidra (10) and sukuti (10) were collected and were analysed for microbial load. population of lactic acid bacteria (lab) as well as aerobic mesophilic counts ranged from 4.7-8.3 to 5.1-8.5 log cfu g(-1), respectively. a total of 189 strains of lab were isolated from sukako maacha, gnuchi, sidra and ...	2006	16278028
characterisation of the microflora of attiéké, a fermented cassava product, during traditional small-scale preparation.	attiéké is a fermented cassava product consumed mainly in cote d'ivoire. the aim of this study was to characterise the attiéké fermentation by examining products from 15 small-scale production sites at various stages of its preparation. for the preparation of attiéké, fresh cassava is grated to a pulp and inoculated with 10% of a spontaneous traditional inoculum. the inocula contained aerobic mesophiles at mean numbers of 8.2 x 10(7) cfu/g and lactic and acetic acids at mean concentrations of 0. ...	2006	16213052
potential application of ribosomal intergenic spacer analysis to the microbial community analysis of agronomic products.	ribosomal intergenic spacer analysis (risa) has been applied to the microbial community analysis of agronomic products in combination with a simple and rapid dna extraction method, consisting of a one-step extraction and two-step purification, for a variety of agronomic products. risa appears to be a useful tool for the study of the community structures of food-associated microbes and their use as a unique fingerprinting signature for each agronomic product. sequencing analyses of amplicons gene ...	2005	15998122
analysis of kimchi microflora using denaturing gradient gel electrophoresis.	a polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) technique was used to determine the microfloral composition during the fermentation of kimchi, a traditional korean fermented vegetable food. the kimchi was fermented at 10 degrees c or 20 degrees c for 30 or 20 days, respectively. dgge of the partially amplified 16s rdna was performed and the most intense bands sequenced. the application of this culture-independent molecular technique determined that the lactic acid ...	2005	15992614
characterization and identification of lactic acid bacteria in "morcilla de burgos".	a total of 176 lactic acid bacteria (lab) isolated from a typical spanish blood sausage called "morcilla de burgos" were identified by means of phenotypic characteristics and 16s rdna rflp (ribotyping). lab were isolated from "morcilla" of different producers and in different storage periods, which includes unpackaged, vacuum and modified atmosphere packaged "morcilla" and vacuum packed and pasteurised "morcilla". the knowledge of specific spoilage bacteria of "morcilla de burgos" will be useful ...	2005	15582739
weissella confusa infection in primate (cercopithecus mona).		2003	14626220
novel leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product.	to determine the dominant microorganisms involved in kimchi fermentation and to examine their effect on kimchi fermentation, we randomly isolated and characterized 120 lactic acid bacteria from kimchi during a 5-day fermentation at 15 degrees c. leuconostoc citreum was dominant during the early and mid-phases of kimchi fermentation whereas lactobacillus sake/lactobacillus curvatus or lactobacillus brevis were found during later stages. eighty-two out of 120 isolates (68%) were identified as leuc ...	2003	14574101
fatal case of endocarditis due to weissella confusa.	this is the first reported case of endocarditis due to the lactobacillus-like vancomycin-resistant gram-positive bacillus weissella confusa. full identification and susceptibility testing of lactobacillus-like organisms recovered in blood culture should be performed for patients with clinical presentations that suggest endocarditis.	2003	12734290
isolation, characterisation and identification of lactic acid bacteria from bushera: a ugandan traditional fermented beverage.	one hundred and thirteen strains of lactic acid bacteria (lab) were selected from 351 isolates from 15 samples of traditionally fermented household bushera from uganda and also from laboratory-prepared bushera. isolates were phenotypically characterised by their ability to ferment 49 carbohydrates using api 50 chl kits and additional biochemical tests. coliforms, yeasts and lab were enumerated in bushera. the ph, volatile organic compounds and organic acids were also determined. the lab counts i ...	2003	12494920
microbiological and fermentation characteristics of togwa, a tanzanian fermented food.	selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ...	2003	12423921
a pcr-based method for identification of lactobacilli at the genus level.	we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ...	2002	12351242
effect of weissella confusa strain pl9001 on the adherence and growth of helicobacter pylori.	viable and nonviable weissella confusa strain pl9001 inhibited the binding of helicobacter pylori to human gastric-cell line mkn-45 cells by more than 90%. spent culture supernatant of pl9001 rapidly decreased the viability of h. pylori, rupturing cell walls. the results suggest that pl9001 is a probiotic that can reduce the infectivity and persistence of h. pylori.	2002	12200324
weissella soli sp. nov., a lactic acid bacterium isolated from soil.	phylogenetic analysis of the 16s rrna gene of bacterial isolates from garden soil showed relatedness to weissella kandleri and weissella confusa. however, the sequences had notable differences, and dna-dna hybridizations confirmed that the isolates are separate from these two species. the isolates could be further distinguished from all previously described weissella species by electrophoretic analysis of whole-cell proteins, as well as by the results from different biochemical tests. the name w ...	2002	12054246
isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barley cultivars.	malt has a complex microbial population, which changes as the malting process commences. little is known about the proliferation of lactic acid bacteria (lab) in each of the malting phases. in this study, we determined the number of lab present in the different phases of malting with clipper and prisma barley cultivars. the strains were identified to species level by using numerical analysis of total soluble cell protein patterns, rapd-pcr banding patterns and 16s rrna sequencing. the number of ...	2002	12038579
weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi.	a gram-positive, catalase-negative, non-sporulating, facultatively anaerobic, short rod-shaped bacterium, with cells measuring 0.3-0.5 x 1-2 microm and designated strain chj3t, was isolated from partially fermented kimchi, a traditional korean fermented vegetable food. the strain produced co2 gas, d-lactate from glucose and dextran from sucrose and hydrolysed aesculin and arginine. it also fermented n-acetylglucosamine, amygdalin, arbutin, cellobiose, d-fructose, galactose, beta-gentiobiose, glu ...	2002	11931163
taxonomic study of weissella confusa and description of weissella cibaria sp. nov., detected in food and clinical samples.	a taxonomic study was conducted to clarify the relationships of two bacterial populations belonging to the genus weissella. a total of 39 strains originating mainly from malaysian foods (22 strains) and clinical samples from humans (9 strains) and animals (6 strains) were analysed using a polyphasic taxonomic approach. the methods included classical phenotyping, whole-cell protein electrophoresis, 16s and 23s rdna rflp (ribotyping), determination of 16s rdna sequence homologies and dna-dna reass ...	2002	11837296
weissella confusa (basonym: lactobacillus confusus) bacteremia: a case report.	infection with lactobacillus is rare, and only a handful of species have been identified as being clinically significant: lactobacillus casei, lactobacillus rhamnosus, and lactobacillus leichmannii. the literature contains one case report of bacteremia caused by weissella confusa (basonym: lactobacillus confusus), but the clinical significance of the infection was unclear. we describe a case of w. confusa bacteremia in a 46-year-old man with a history of abdominal aortic dissection and repair. t ...	2001	11283096
phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species triticum durum and triticum aestivum) sourdoughs of southern italy.	the microflora of 25 wheat sourdoughs from the apulia region, southern italy, was characterized. the sourdoughs were mainly produced from triticum durum wheat. the number of lactic acid bacteria and yeasts ranged from ca. log 7.5 to log 9.3 colony forming units (cfu)/g and from log 5.5 to log 8.4 cfu/g, respectively. about 38% of the 317 isolates of lactic acid bacteria were identified by conventional physiological and biochemical tests. phenotypic identification was confirmed by 16s rdna and 16 ...	2001	11252516
identification of lactic acid bacteria from chili bo, a malaysian food ingredient.	ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ...	1999	9925588
modified substrate specificity of l-hydroxyisocaproate dehydrogenase derived from structure-based protein engineering.	l-2-hydroxyisocaproate dehydrogenase (l-hicdh) is characterized by a broad substrate specificity and utilizes a wide range of 2-oxo acids branched at the c4 atom. modifications have been made to the sequence of the nad(h)-dependent l-hicdh from lactobacillus confusus in order to define and alter the region of substrate specificity towards various 2-oxocarbonic acids. all variations were based on a 3d-structure model of the enzyme using the x-ray coordinates of the functionally related l-lactate ...	1997	9153075
munkoyo beverage, a traditional zambian fermented maize gruel using rhynchosia root as amylase source.	a typical munkoyo beverage was made by fermenting rhynchosia heterophylla root extract-cooked maize meal mixture with lactobacillus confusus lzi and saccharomyces cerevisiae yz20. the fermented munkoyo beverage had a ph of 3.3, lactic acid content of 60 mmol/l, ethanol 320-410 mmol/l and a characteristic 'munkoyo' aroma. l. confusus, used alone, produced a beverage with a faint munkoyo flavour note whilst beverage produced with s. cerevisiae alone seemed not to have a typical munkoyo note. r. he ...	1997	9039570
lactobacillus- and bifidobacterium-mediated antigenotoxicity in the colon of rats.	lactic acid bacteria (lab) are proposed to have several beneficial effects, including the inactivation of carcinogens. we have studied the potential of lactobacillus acidophilus (from a commercially available yogurt), lactobacillus gasseri (p79), lactobacillus confusus (dsm20196), streptococcus thermophilus (ncim 50083), bifidobacterium breve and bifidobacterium longum (from human infant stool) to prevent the induction of dna damage by n-methyl-n'-nitro-n-nitrosoguanidine (mnng, 7.5 mg/kg body w ...	1996	8910918
deletion variants of l-hydroxyisocaproate dehydrogenase. probing substrate specificity.	the substrate specificity and catalytic activity of the dinucleotide-dependent l-2-hydroxyisocaproate dehydrogenase from lactobacillus confusus (l-hicdh) have been altered by modifying an enzyme region which is assumed to be involved in substrate recognition. the design of the variant enzymes was based on an amino acid alignment of the modified region with the functionally related l-lactate dehydrogenases. the best absolute sequence similarity for a protein with known tertiary structure was foun ...	1994	8055963
phylogenetic analysis of some leuconostocs and related organisms as determined from large-subunit rrna gene sequences: assessment of congruence of small- and large-subunit rrna derived trees.	the large-subunit ribosomal rna gene sequences of leuconostoc carnosum, leuc. mesenteroides, leuc. oenos, leuc. paramesenteroides and lactobacillus confusus were determined by direct sequencing of enzymatically-amplified dna. comparative analysis of the large-subunit rdna sequences revealed that the leuconostocs form three distinct lines of descent: leuconostoc sensu stricto, leuc. paramesenteroides group of organisms, and leuc. oenos. the distance matrix tree constructed from the large-subunit ...	1993	7683639
crystal structure of l-2-hydroxyisocaproate dehydrogenase from lactobacillus confusus at 2.2 a resolution. an example of strong asymmetry between subunits.	l-2-hydroxyisocaproate dehydrogenase (l-hicdh) from lactobacillus confusus, a homotetramer with a molecular mass of 33 kda per subunit, belongs to the protein family of the nad(+)-dependent l-2-hydroxycarboxylate dehydrogenases. l-hicdh was crystallized with ammonium sulphate as precipitant in the presence of nad+. the crystals belong to the trigonal space group p3(2)21, with a = 135.9 a and c = 205.9 a, and diffract x-rays to 2.2 a resolution. the crystal structure was solved by patterson searc ...	1995	7643402
[taxonomy of the species lactobacillus beijerinck. vi. lactobacillus coprophilus subsp. confusus nov. subsp., a new variety of the subspecies betabacterium].		1969	4913652
some slime-forming heterofermentative species of the genus lactobacillus.	lactobacillus coprophilus subsp. confusus (ncdo 1586) and 18 other strains of slime-forming heterofermentative lactobacilli obtained from diverse sources are considered to form a new species on the basis of their physiological characteristics and similarities of their pyruvate reductases and lactate dehydrogenases. it is suggested that these strains should be named lactobacillus confusus (holzapfel & kandler) comb. nov. (l. coprophilus subsp. confusus holzapfel & kandler), reasons being given. t ...	1972	4259626
identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria.	a total of 84 of 150 vancomycin-resistant (defined as no inhibition of bacterial growth around a 30-micrograms vancomycin disk placed on 5% sheep blood-trypticase soy agar [bbl microbiology systems, cockeysville, md.]) bacteria were definitively identified by determining the phenotypic criteria. the identity of representatives was also confirmed by dna-dna hybridizations. the following strains were identified: 1 enterococcus faecium, 18 leuconostoc mesenteroides, 15 leuconostoc citreum, 9 leucon ...	1989	2723037
cloning, sequencing and expression of the l-2-hydroxyisocaproate dehydrogenase-encoding gene of lactobacillus confusus in escherichia coli.	the gene (l-hicdh) encoding l-2-hydroxyisocaproate dehydrogenase (l-hicdh) from lactobacillus confusus was cloned in escherichia coli. a 69-mer oligodeoxyribonucleotide probe, derived to be complementary to the n-terminal amino acid (aa) coding sequence, was used for screening. the complete nucleotide (nt) sequence of the l-hicdh gene was determined. the 5'-end of the mrna was mapped by primer extension and the promoter identified. downstream from the l-hicdh gene is a typical rho-independent te ...	1989	2684788
clinical and microbiologic characteristics of pediococci.	over a 43-month period, 23 separate isolates of nonenterococcal alpha- and nonhemolytic streptococci were reported by our clinical microbiology laboratory to be resistant to vancomycin. this constituted 0.32% of nonenterococcal alpha- and nonhemolytic streptococci reported and 4.4% of such streptococci upon which susceptibility testing was performed. of 13 isolates which were available for further study, all were highly resistant to vancomycin (mic greater than or equal to 1,024 micrograms/ml), ...	1990	2380362
recovery of vancomycin-resistant gram-positive cocci from children.	a cross-sectional survey of vancomycin-resistant gram-positive cocci (vrgpc) in the feces of children was initiated after several bacteremic infections with these organisms occurred at our hospital. a selective medium consisting of colistin-nalidixic acid agar, 5% sheep blood, vancomycin (5 mg/liter), and amphotericin b (8 mg/liter) was developed to isolate vrgpc. a single stool specimen submitted to the clinical microbiology laboratory from each of 48 patients was inoculated onto the medium. pl ...	1990	2108993
abscess caused by vancomycin-resistant lactobacillus confusus.	several isolates of vancomycin-resistant lactobacillus confusus from human sources have been described, but to our knowledge, no well-documented infection attributable to this organism has been published. a thumb abscess caused by this bacterium in a healthy 49-year-old male is reported here. he was successfully treated by surgical drainage and cephalothin.	1991	1774335
recovery of vancomycin-resistant gram-positive cocci from pediatric liver transplant recipients.	between november 1988 and october 1989, 49 first-time pediatric liver transplant recipients at the children's hospital of pittsburgh were prospectively monitored for the presence of stool colonization and the development of disease caused by vancomycin-resistant gram-positive cocci (vrgpc). quantitative stool culturing was done on a weekly basis, and cultures were planted onto a selective medium for vrgpc. isolates for which the mic was greater than or equal to 8 were considered resistant to van ...	1991	1774255
nucleotide sequence of 16s ribosomal rna from lactobacillus viridescens and lactobacillus confusus.		1990	1694026
transformation of n epsilon-cbz-l-lysine to cbz-l-oxylysine using l-amino acid oxidase from providencia alcalifaciens and l-2-hydroxy-isocaproate dehydrogenase from lactobacillus confusus.	biotransformations were developed to oxidize n epsilon-carbobenzoxy(cbz)-l-lysine and to reduce the product keto acid to l-cbz-oxylysine. lysyl oxidase (l-lysine: o2 oxidoreductase, ec 1.4.3.14) from trichoderma viride was relatively specific for l-lysine and had very low activity with n epsilon-substituted derivatives. l-amino acid oxidase (l-amino acid: o2 oxidoreductase [deaminating], ec 1.4.3.2) from crotalus adamanteus venom had low activity with l-lysine but high activity with n epsilon-fo ...	1992	1368913