Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| bacterial biota of nigeen lake waters (kashmir valley). | one of the greatest apprehensions of water consumers all over the world with respect to the quality of drinking water is its contamination with pathogenic microorganisms. this research work determined the potential bacterial contaminants of the waters of nigeen lake, a subsidiary of dal lake and is regarded as a separate lake in kashmir. the study was carried out from may 2014 to november 2014 excluding august and september at four different sites. during the study the bacterial flora showed var ... | 2016 | 27165539 |
| increasing the catalytic activity of bilirubin oxidase from bacillus pumilus: importance of host strain and chaperones proteins. | aggregation of recombinant proteins into inclusion bodies (ibs) is the main problem of the expression of multicopper oxidase in escherichia coli. it is usually attributed to inefficient folding of proteins due to the lack of copper and/or unavailability of chaperone proteins. the general strategies reported to overcome this issue have been focused on increasing the intracellular copper concentration. here we report a complementary method to optimize the expression in e. coli of a promising bilir ... | 2016 | 27165502 |
| a phosphate starvation-inducible ribonuclease of bacillus licheniformis. | the bli03719 protein of bacillus licheniformis dsm13 belongs to the most abundant extracellular proteins under phosphate starvation conditions. in this study, the function of this phosphate starvation inducible protein was determined. an amino-acid sequence analysis of the bli03719-encoding gene showed a high similarity with genes encoding the barnase of bacillus amyloliquefaciens fzb42 and binase-like rnase of bacillus pumilus sarf-032. the comparison of the control strain and a bli03719-defici ... | 2016 | 27160576 |
| plantazolicin is an ultra-narrow spectrum antibiotic that targets the bacillus anthracis membrane. | plantazolicin (pzn) is a ribosomally synthesized and post-translationally modified natural product from bacillus methylotrophicus fzb42 and bacillus pumilus. extensive tailoring to twelve of the fourteen amino acid residues in the mature natural product endows pzn with not only a rigid, polyheterocyclic structure, but also antibacterial activity. here we report a remarkably discriminatory activity of pzn toward bacillus anthracis, which rivals a previously-described gamma (γ) phage lysis assay i ... | 2016 | 27152321 |
| digital data for quick response (qr) codes of alkalophilic bacillus pumilus to identify and to compare bacilli isolated from lonar crator lake, india. | microbiologists are routinely engaged isolation, identification and comparison of isolated bacteria for their novelty. 16s rrna sequences of bacillus pumilus were retrieved from ncbi repository and generated qr codes for sequences (fasta format and full gene bank information). 16srrna were used to generate quick response (qr) codes of bacillus pumilus isolated from lonar crator lake (19° 58' n; 76° 31' e), india. bacillus pumilus 16s rrna gene sequences were used to generate cgr, fcgr and pca. t ... | 2016 | 27141529 |
| cationic antimicrobial peptides derived from crocodylus siamensis leukocyte extract, revealing anticancer activity and apoptotic induction on human cervical cancer cells. | known antimicrobial peptides kt2 and rt2 as well as the novel rp9 derived from the leukocyte extract of the freshwater crocodile (crocodylus siamensis) were used to evaluate the ability in killing human cervical cancer cells. rp9 in the extract was purified by a combination of anion exchange column and reversed-phase hplc, and its sequence was analyzed by mass spectrometry. the novel peptide could inhibit gram-negative vibrio cholerae (clinical isolation) and gram-positive bacillus pumilus tistr ... | 2016 | 27129462 |
| a bacillus sp. isolated from sediments of the sarno river mouth, gulf of naples (italy) produces a biofilm biosorbing pb(ii). | a pb-resistant bacterial strain (named hereinafter pb15) has been isolated from highly polluted marine sediments at the sarno river mouth, italy, using an enrichment culture to which pb(ii) 0.48mmoll(-1) were added. 16s rrna gene sequencing (sanger) allowed assignment of the isolate to the genus bacillus, with bacillus pumilus as the closest species. the isolate is resistant to pb(ii) with a minimum inhibitory concentration (mic) of 4.8mmoll(-1) and is also resistant to cd(ii) and mn(ii) with mi ... | 2016 | 27110973 |
| identification of phenazine-1-carboxylic acid gene (phc cd) from bacillus pumilus mtcc7615 and its role in antagonism against rhizoctonia solani. | bacillus pumilus mtcc7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to rhizoctonia solani, the pathogen causing sheath blight disease of rice. the phenazine-1-carboxylic acid gene (phc cd) of this bacterium was pcr amplified (1400 bp), cloned, and sequenced. the sequence analysis revealed the presence of two orfs of phc cd gene commonly found in pseudomonas species. the sequence showed 98% similarity to phc cd gene of the pseudomonas isolate lbum223 ... | 2016 | 27106067 |
| [characterization of β-1, 4-mannanase from bacillus pumilus and heterologous expression in lactobacillus casei]. | lactobacillus casei is widely used in food production and feed industry. the aim of this study was to construct the recombinant expression mannanase lb. casei. | 2015 | 27101700 |
| diversity of indigenous endophytic bacteria associated with the roots of chinese cabbage (brassica campestris l.) cultivars and their antagonism towards pathogens. | the study aimed to reveal the diversity of endophytic bacteria in the roots of chinese cabbage (cc) cultivated in two areas in korea, namely, seosang-gun (ss) and haenam-gun (hn), and also in a transgenic plant (tp) from the laboratory. a total of 653 colonies were isolated from the interior of cc roots, comprising 118, 302, and 233 isolates from ss, hn, and tp samples, respectively. based on 16s rrna gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-g+c gram- ... | 2016 | 27095454 |
| direct inhibition of oncogenic kras by bacillus pumilus ribonuclease (binase). | ras proteins function as molecular switches that transmit signals from cell surface receptors into specific cellular responses via activation of defined signaling pathways (fang, 2015). aberrant constitutive ras activation occurs with high incidence in different types of cancer (bos, 1989). thus, inhibition of ras-mediated signaling is extremely important for therapeutic approaches against cancer. here we showed that the ribonuclease (rnase) binase, directly interacts with endogenous kras. furth ... | 2016 | 27066977 |
| identification and pathogenic potential of clinical bacillus and paenibacillus isolates. | the soil-related bacillus and paenibacillus species have increasingly been implicated in various human diseases. nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of bacillus anthracis and bacillus cereus, little is known on their pathogenicity for humans. in this study, we evaluated the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry (maldi-tof ms) in the identification of clinical isolates ... | 2016 | 27031639 |
| heterologous expression and characterization of a new heme-catalase in bacillus subtilis 168. | reactive oxygen species (ros) is an inherent consequence to all aerobically living organisms that might lead to the cells being lethal and susceptible to oxidative stress. bacillus pumilus is characterized by high-resistance oxidative stress that stimulated our interest to investigate the heterologous expression and characterization of heme-catalase as potential biocatalyst. results indicated that recombinant enzyme significantly exhibited the high catalytic activity of 55,784 u/mg expressed in ... | 2016 | 27016935 |
| mixed phenolic acids mediated proliferation of pathogens talaromyces helicus and kosakonia sacchari in continuously monocultured radix pseudostellariae rhizosphere soil. | radix pseudostellariae l. is a common and popular chinese medication. however, continuous monoculture has increased its susceptibility to severe diseases. we identified two pathogenic microorganisms, talaromyces helicus m. (ku355274) and kosakonia sacchari w. (ku324465), and their antagonistic bacterium, bacillus pumilus z. in rhizosphere soil of continuously monocultured r. pseudostellariae. nine types of phenolic acids were identified both in the rhizosphere soil and in culture medium under st ... | 2016 | 27014250 |
| plant growth-promoting rhizobacteria inoculation to enhance vegetative growth, nitrogen fixation and nitrogen remobilisation of maize under greenhouse conditions. | plant growth-promoting rhizobacteria (pgpr) may provide a biological alternative to fix atmospheric n2 and delay n remobilisation in maize plant to increase crop yield, based on an understanding that plant-n remobilisation is directly correlated to its plant senescence. thus, four pgpr strains were selected from a series of bacterial strains isolated from maize roots at two locations in malaysia. the pgpr strains were screened in vitro for their biochemical plant growth-promoting (pgp) abilities ... | 2016 | 27011317 |
| [inhibition of bacillus pumilus ar03 on alternaria alternata and erysiphe cichoracearum on tobacco]. | this study aimed to evaluate the efficiency of bacillus pumilus ar03 against altenaria alternata and erysiphe cichoracearum. the antagonistic activities were studied in the way of co-culture on plate, inhibition of pathogen conidia germination on concave slides and lb agar medium. in the investigation, the water suspension of living cells of strain ar03, at 3 x 10(8) cfu . ml-1 had a remarkable inhibition effect on hyphae growth and conidia germination of a. alternata and caused hyphae deformati ... | 2015 | 26995927 |
| growth and (137)cs uptake and accumulation among 56 japanese cultivars of brassica rapa, brassica juncea and brassica napus grown in a contaminated field in fukushima: effect of inoculation with a bacillus pumilus strain. | fifty six local japanese cultivars of brassica rapa (40 cultivars), brassica juncea (10 cultivars) and brassica napus (6 cultivars) were assessed for variability in growth and (137)cs uptake and accumulation in association with a bacillus pumilus strain. field trial was conducted at a contaminated farmland in nihonmatsu city, in fukushima prefecture. inoculation resulted in different responses of the cultivars in terms of growth and radiocesium uptake and accumulation. b. pumilus induced a signi ... | 2016 | 26986237 |
| [alkaline-adapted beta-mannanase of bacillus pumilus: gene heterologous expression and enzyme characterization]. | we expressed a novel alkaline-adapted beta-mannanase gene and characterized the enzyme for potential industrial applications. | 2015 | 26915226 |
| cost-effective and concurrent production of industrially valuable xylano-pectinolytic enzymes by a bacterial isolate bacillus pumilus ajk. | simultaneous production of xylanase and pectinase by bacillus pumilus ajk under submerged fermentation was investigated in this study. under optimized conditions, it produced 315 ± 16 iu/ml acidic xylanase, 290 ± 20 iu/ml alkaline xylanase, and 88 ± 9 iu/ml pectinase. the production of xylano-pectinolytic enzymes was the highest after inoculating media (containing 2% each of wheat bran and citrus limetta peel, 0.5% peptone, 10 mm mgso4, ph 7.0) with 2% of 21-hr-old culture and incubated at 37°c ... | 2017 | 26914524 |
| identification of bacillus probiotics isolated from soil rhizosphere using 16s rrna, reca, rpob gene sequencing and rapd-pcr. | some bacillus species, especially bacillus subtilis and bacillus pumilus groups, have highly similar 16s rrna gene sequences, which are hard to identify based on 16s rdna sequence analysis. to conquer this drawback, rpob, reca sequence analysis along with randomly amplified polymorphic (rapd) fingerprinting was examined as an alternative method for differentiating bacillus species. the 16s rrna, rpob and reca genes were amplified via a polymerase chain reaction using their specific primers. the ... | 2016 | 26898909 |
| construction and characterization of three protein-targeting expression system in lactobacillus casei. | we previously reported that the β-1,4-mannanase (manb) gene from bacillus pumilus functions as a good reporter gene in lactobacillus casei. two vectors were constructed. one carries the signal peptide of secretion protein usp45 (spusp45) from lactococcus lactis (pelsh), and the other carries the full-length s-layer protein, slpa, from l. acidophilus (pelwh). in this work, another vector, pelsph, was constructed to include the signal peptide of protein slpa (spslpa), and the capacity of all three ... | 2016 | 26892019 |
| genome sequence of bacillus pumilus strain bonn, isolated from an anthrax-like necrotic skin infection site of a child. | we report the draft genome sequence of bacillus pumilus strain bonn associated with human skin infection. b. pumilus bonn was isolated from a carbuncle-like necrotic site, resembling cutaneous anthrax, on the back of the hand of a 10-year-old child. | 2016 | 26868410 |
| regioselective deprotection of the monosaccharide-bearing thiocyanomethyl group at the anomeric position monitored by reversed-phase hplc method. | in the current work, the investigation and development of a chemo-enzymatic approach for the synthesis of neo-glycoproteins have been studied. this strategy is based on the regioselective enzymatic hydrolysis of peracetylated monosaccharide, functionalized at the anomeric position (c1) as 1-thio-(s-cyanomethyl) group, a precursor of the 2- iminomethoxyethyl thioglycosides-linker for protein glycosylation, catalyzed by immobilized enzymes to obtain selectively monodeprotected compounds. the use o ... | 2016 | 26864255 |
| analysis of bacterial communities associated with potting media. | potting media are commonly used by growers in different parts of the world for potted plants, raising seedlings and for improving soil characteristics. this study was conducted to characterize bacterial communities occurring in 13 commercial potting media products originating from seven countries. | 2016 | 26844021 |
| thermostable xanthine oxidase activity from bacillus pumilus rl-2d isolated from manikaran thermal spring: production and characterization. | xanthine oxidase is an important enzyme of purine metabolism that catalyzes the hydroxylation of hypoxanthine to xanthine and then xanthine to uric acid. a thermostable xanthine oxidase is being reported from a thermophilic organism rl-2d isolated from the manikaran (kullu) hot spring of himachal pradesh (india). based on the morphology, physiological tests, and 16s rdna gene sequence, rl-2d was identified as bacillus pumilus. optimization of physiochemical parameters resulted into 4.1-fold incr ... | 2016 | 26843701 |
| cell wall as a target for bacteria inactivation by pulsed electric fields. | the integrity and morphology of bacteria is sustained by the cell wall, the target of the main microbial inactivation processes. one promising approach to inactivation is based on the use of pulsed electric fields (pef). the current dogma is that irreversible cell membrane electro-permeabilisation causes the death of the bacteria. however, the actual effect on the cell-wall architecture has been poorly explored. here we combine atomic force microscopy and electron microscopy to study the cell-wa ... | 2016 | 26830154 |
| applicability of uv resistant bacillus pumilus endospores as a human adenovirus surrogate for evaluating the effectiveness of virus inactivation in low-pressure uv treatment systems. | recent studies have demonstrated the potential to use bacillus pumilus endospores as a surrogate for human adenovirus (hadv) in uv disinfection studies. the use of endospores has been limited by observations of batch-to-batch variation in uv sensitivity. this study reports on a propagation method that utilizes a commercially available medium to produce uv tolerant b. pumilus endospores with a consistent uv sensitivity. it is further demonstrated that the endospores of b. pumilus strain (atcc 271 ... | 2016 | 26825005 |
| purification and partial characterization of serine-metallokeratinase from a newly isolated bacillus pumilus nrc21. | a serine metallokeratinase enzyme (30 kda) produced by a newly isolated bacillus strain (bacillus pumilus nrc21) cultivated under optimized conditions in medium containing chicken feather meal was purified and characterized in a set of biochemical assays. the purification was carried out using two successive chromatographic steps; cation exchange chromatography on cm-cellulose and gel filtration on sephadex g-100 columns. the purified enzyme showed a specific activity of 2000 units/mg protein ag ... | 2016 | 26802243 |
| an in-depth characterization of the entomopathogenic strain bacillus pumilus 15.1 reveals that it produces inclusion bodies similar to the parasporal crystals of bacillus thuringiensis. | in the present work, the local isolate bacillus pumilus 15.1 has been morphologically and biochemically characterized in order to gain a better understanding of this novel entomopathogenic strain active against ceratitis capitata. this strain could represent an interesting biothechnological tool for the control of this pest. here, we report on its nutrient preferences, extracellular enzyme production, motility mechanism, biofilm production, antibiotic suceptibility, natural resistance to chemica ... | 2016 | 26782747 |
| aerobic and anaerobic microbial degradation of crude (4-methylcyclohexyl)methanol in river sediments. | cyclohexane and some of its derivatives have been a major concern because of their significant adverse human health effects and widespread occurrence in the environment. the 2014 west virginia chemical spill has raised public attention to (4-methylcyclohexyl)methanol (4-mchm), one cyclohexane derivative, which is widely used in coal processing but largely ignored. in particular, the environmental fate of its primary components, cis- and trans-4-mchm, remains largely unexplored. this study aimed ... | 2016 | 26780132 |
| probing an interfacial surface in the cyanide dihydratase from bacillus pumilus, a spiral forming nitrilase. | nitrilases are of significant interest both due to their potential for industrial production of valuable products as well as degradation of hazardous nitrile-containing wastes. all known functional members of the nitrilase superfamily have an underlying dimer structure. the true nitrilases expand upon this basic dimer and form large spiral or helical homo-oligomers. the formation of this larger structure is linked to both the activity and substrate specificity of these nitrilases. the sequences ... | 2015 | 26779137 |
| catalytic, kinetic and thermodynamic properties of bacillus pumilus fh9 keratinase conjugated with activated pectin. | bacillus pumilus fh9 keratinase was covalently coupled to several oxidized polysaccharides. the conjugates were evaluated for the retained activity, kinetic and thermodynamic stability. among all preparations, the conjugated enzyme with oxidized pectin had the highest recovered activity (71.75%) and the highest thermal stability at 60°c (t1/2=333 min). compared to the native enzyme, the conjugated preparation exhibited higher optimum temperature, lower activation energy (ea), lower deactivation ... | 2016 | 26743746 |
| bacillus zhangzhouensis sp. nov. and bacillus australimaris sp. nov. | two strains, designated dw5-4t and nh7i_1t, were isolated from aquaculture water of shrimp farm of zhangzhou city in china and surface sediment of the south china sea, respectively. cells of the strains were gram-stain-positive, oxidase- and catalase-positive, rod-shaped and motile. the phylogenetic analyses based on 16s rrna gene sequences indicated that two strains belonged to the bacillus pumilus clade. the similarities of 16s rrna gene sequences between two strains dw5-4t and nh7i_1t was 99. ... | 2015 | 26705002 |
| identification of bacillus strains by maldi tof ms using geometric approach. | microorganism identification by maldi tof mass-spectrometry is based on the comparison of the mass spectrum of the studied organism with those of reference strains. it is a rapid and reliable method. however, commercial databases and programs are mostly designed for identification of clinically important strains and can be used only for particular mass spectrometer models. the need for open platforms and reference databases is obvious. in this study we describe a geometric approach for microorga ... | 2015 | 26592761 |
| sensitive change of iso-branched fatty acid (iso-15:0) in bacillus pumilus pamc 23174 in response to environmental changes. | in this study, the environmental adaptive metabolic processes were investigated using a psychrotrophic polar bacterium bacillus pumilus pamc 23174 in response to various temperatures and nutrients, especially in regard to the synthesis of fatty acids. fatty acid methyl ester analysis was performed using gas chromatography-mass spectrometry and we found that a sensitive changes in iso-branched fatty acid (iso-15:0) synthesis occurred when adjusting the nutritional ratio of branched chain fatty ac ... | 2016 | 26566954 |
| heterologous expression of mannanase and developing a new reporter gene system in lactobacillus casei and escherichia coli. | reporter gene systems are useful for studying bacterial molecular biology, including the regulation of gene expression and the histochemical analysis of protein products. here, two genes, β-1,4-mannanase (manb) from bacillus pumilus and β-glucuronidase (gusa) from escherichia coli k12, were cloned into the expression vector pelx1. the expression patterns of these reporter genes in lactobacillus casei were investigated by measuring their enzymatic activities and estimating their recombinant prote ... | 2015 | 26562012 |
| detection of boron removal capacities of different microorganisms in wastewater and effective removal process. | in this study boron removal capacities of different microorganisms were tested. candida tropicalis, rhodotorula mucilaginosa, micrococcus luteus, bacillus thuringiensis, bacillus cereus, bacillus megaterium, bacillus pumilus, pseudomonas aeruginosa and aspergillus versicolor were examined for their boron bioaccumulation capacities in simulated municipal wastewater. a. versicolor and b. cereus were found as the most boron-tolerant microorganisms in the experiments. also boron bioaccumulation yiel ... | 2015 | 26540546 |
| chitin from penaeus merguiensis via microbial fermentation processing and antioxidant activity. | the ability of three proteases producing bacteria such as pseudomonas aeruginosa, serratia marcescens, and bacillus pumilus on the demineralization and deproteinzation efficiency of shrimp waste, for chitin extraction, was investigated. statistical analysis of data was showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). the highest deproteinization (74.76%) and demineralization rate (78.46%) was obtained with p. ... | 2016 | 26519877 |
| isolation and characterization of two novel psychrotrophic decabromodiphenyl ether-degrading bacteria from river sediments. | decabromodiphenyl ether (bde-209) is a brominated flame retardant and a priority contaminant. currently, little information is available about its significance in the environment, specifically about its susceptibility to aerobic biotransformation at low temperature. in this work, five phylogenetically diverse bde-209-degrading bacterial strains were isolated from river sediments of northern china. these strains were distributed among four different genera-acinetobacter, pseudomonas, bacillus and ... | 2016 | 26517991 |
| matrix production, pigment synthesis, and sporulation in a marine isolated strain of bacillus pumilus. | the ability to produce an extracellular matrix and form multicellular communities is an adaptive behavior shared by many bacteria. in bacillus subtilis, the model system for spore-forming bacteria, matrix production is one of the possible differentiation pathways that a cell can follow when vegetative growth is no longer feasible. while in b. subtilis the genetic system controlling matrix production has been studied in detail, it is still unclear whether other spore formers utilize similar mecha ... | 2015 | 26506360 |
| structural characterization and antimicrobial activity of a biosurfactant obtained from bacillus pumilus dsvp18 grown on potato peels. | biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. they are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. major limiting factor in biosurfactant production is their production cost. | 2015 | 26495106 |
| studies on properties of the xylan‑binding domain and linker sequence of xylanase xyng1‑1 from paenibacillus campinasensis g1‑1. | xylanase xyng1-1 from paenibacillus campinasensis g1-1 consists of a catalytic domain (cd), a family 6_36 carbohydrate-binding module which is a xylan-binding domain (xbd), and a linker sequence (ls)between them. the structure of xyng1-3 from bacillus pumilus g1-3 consists only of a cd. to investigate the functions and properties of the xbd and ls of xyng1-1, two truncated forms (xyng1-1cdl, xyng1-1cd) and three fusion derivatives (xyng1-3cdl, xyng1-3cdx and xyng1-3cdlx) were constructed and bio ... | 2015 | 26467249 |
| sterilization resistance of bacterial spores explained with water chemistry. | bacterial spores can survive for long periods without nutrients and in harsh environmental conditions. this survival is influenced by the structure of the spore, the presence of protective compounds, and water retention. these compounds, and the physical state of water in particular, allow some species of bacterial spores to survive sterilization schemes with hydrogen peroxide and uv light. the chemical nature of the spore core and its water has been a subject of some contention and the chemical ... | 2015 | 26435315 |
| identification, sequencing and comparative analysis of pbp15.s plasmid from the newly described entomopathogen bacillus pumilus 15.1. | the bacillus pumilus 15.1 strain, a recently described entomopathogenic strain active against ceratitis capitata, contains at least two extrachromosomal elements, pbp15.1s and pbp15.1b. given that b. pumilus is not a typical entomopathogenic bacterium, the acquisition of this extrachromosomal dna may explain why b. pumilus 15.1 is toxic to an insect. one of the plasmids present in the strain, the pbp15.1s plasmid, was sub-cloned, sequenced and analyzed using bioinformatics to identify any potent ... | 2015 | 26416357 |
| aerobic cyanide degradation by bacterial isolates from cassava factory wastewater. | ten bacterial strains that utilize cyanide (cn) as a nitrogen source were isolated from cassava factory wastewater after enrichment in a liquid media containing sodium cyanide (1 mm) and glucose (0.2% w/v). the strains could tolerate and grow in cyanide concentrations of up to 5 mm. increased cyanide levels in the media caused an extension of lag phase in the bacterial growth indicating that they need some period of acclimatisation. the rate of cyanide removal by the strains depends on the initi ... | 2015 | 26413045 |
| study of the radiation effect of 99mo/99mtc generator on bacillus subtilis and bacillus pumilus species. | in this work, molybdenum-99 loaded columns were challenged with bacillus subtilis vegetative cells and bacillus pumilus spores inside and outside the alumina column, and microbial recovery and radiation effect were assessed. alumina was a barrier for the passage of microorganisms regardless the species, whilst spores were more retained than vegetative cells with a lower microbial recovery, without significant differences between 9.25 and 74 gbq generators. bacillus pumilus biological indicator s ... | 2016 | 26408912 |
| draft genome sequence of the entomopathogenic bacterium bacillus pumilus 15.1, a strain highly toxic to the mediterranean fruit fly ceratitis capitata. | we present the draft whole-genome sequence of the entomopathogenic bacillus pumilus 15.1 strain that consists of 3,795,691 bp and 3,776 predicted protein-coding genes. this genome sequence provides the basis for understanding the potential mechanism behind the toxicity and virulence of b. pumilus 15.1 against the mediterranean fruit fly. | 2015 | 26404596 |
| construction of novel shuttle expression vectors for gene expression in bacillus subtilis and bacillus pumilus. | a native plasmid (psu01) was detected by genome sequencing of bacillus subtilis strain s1-4. two psu01-based shuttle expression vectors psu02-ap and psu03-ap were constructed enabling stable replication in b. subtilis wb600. these vectors contained the reporter gene apre, encoding an alkaline protease from bacillus pumilus ba06. the expression vector psu03-ap only possessed the minimal replication elements (rep, sso, dso) and exhibited more stability on structure, suggesting that the rest of the ... | 2015 | 26377132 |
| sugarcane bagasse as support for immobilization of bacillus pumilus hz-2 and its use in bioremediation of mesotrione-contaminated soils. | the degrading microorganisms isolated from environment usually fail to degrade pollutants when used for bioremediation of contaminated soils; thus, additional treatments are needed to enhance biodegradation. in the present study, the potential of sugarcane bagasse as bacteria-immobilizing support was investigated in mesotrione biodegradation. a novel isolate bacillus pumilus hz-2 was applied in bacterial immobilization, which was capable of degrading over 95 % of mesotrione at initial concentrat ... | 2015 | 26337896 |
| draft genome sequence of the shellfish larval probiotic bacillus pumilus ri06-95. | bacillus pumilus ri06-95 is a marine bacterium isolated in narragansett, rhode island, which has shown probiotic activity against marine pathogens in larval shellfish. we report the genome of b. pumilus ri06-95, which provides insight into the microbe's probiotic ability and may be used in future studies of the probiotic mechanism. | 2015 | 26337873 |
| cyanide hydratases and cyanide dihydratases: emerging tools in the biodegradation and biodetection of cyanide. | the purpose of this study is to summarize the current knowledge of the enzymes which are involved in the hydrolysis of cyanide, i.e., cyanide hydratases (chts; ec 4.2.1.66) and cyanide dihydratases (cynd; ec 3.5.5.1). chts are probably exclusively produced by filamentous fungi and widely occur in these organisms; in contrast, cynds were only found in a few bacterial genera. chts differ from cynds in their reaction products (formamide vs. formic acid and ammonia, respectively). several chts were ... | 2015 | 26329848 |
| microbial selenium nanoparticles (senps) and their application as a sensitive hydrogen peroxide biosensor. | the cell-free extract, a crude enzyme (cytosolic and membrane fraction) obtained from an environmental isolate, bacillus pumilus sp. bab-3706 worked as excellent in reducing as well as stabilizing agent and facilitated the formation of stable colloidal selenium nanoparticles (senps). resulting nanoparticles were characterized using uv-vis spectrophotometer, tem, edax, ft-ir and xrd, respectively. a working electrode was modified by coating the surface of indium tin oxide (ito) with colloidal sen ... | 2015 | 26319569 |
| influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass brachypodium distachyon (l.) p. beauv. | plant growth-promoting rhizobacteria are increasingly being seen as a way of complementing conventional inputs in agricultural systems. the effects on their host plants are diverse and include volatile-mediated growth enhancement. this study sought to assess the effects of bacterial volatiles on the biomass production and root system architecture of the model grass brachypodium distachyon (l.) beauv. | 2015 | 26264238 |
| purification and characterization of [formula: see text]-mannanase from bacillus pumilus (m27) and its applications in some fruit juices. | thermo alkaline mannanase was purified from the bacteria of bacillus pumilus (m27) using the techniques of ammonium sulphate precipitation, deae-sephadex ion exchange chromatography and sephacryl s200 gel filtration chromatography with 111-fold and 36 % yield. it was determined that the enzyme had 2 sub-units including 35 kda and 55 kda in gel filtration chromatography and sds-page electrophoresis systems. the optimum ph and temperature was determined as 8 and 60 °c, respectively. it was also no ... | 2014 | 26243955 |
| a novel thermostable gh5_7 β-mannanase from bacillus pumilus gbsw19 and its application in manno-oligosaccharides (mos) production. | a novel thermostable mannanase from a newly isolated bacillus pumilus gbsw19 has been identified, expressed, purified and characterized. the enzyme shows a structure comprising a 28 amino acid signal peptide, a glycoside hydrolase family 5 (gh5) catalytic domain and no carbohydrate-binding module. the recombinant mannanase has molecular weight of 45 kda with an optimal ph around 6.5 and is stable in the range from ph 5-11. meanwhile, the optimal temperature is around 65 °c, and it retains 50% re ... | 2015 | 26215338 |
| effects of direct-fed bacillus pumilus 8g-134 on feed intake, milk yield, milk composition, feed conversion, and health condition of pre- and postpartum holstein cows. | the usage of direct-fed microbials (dfm) has become common in the dairy industry, but questions regarding choice of strain, mode of action, and efficacy remain prevalent. the objective of this study was to evaluate the effects of a dfm (bacillus pumilus 8g-134) on pre- and postpartum performance and incidence of subclinical ketosis in early lactation. forty-three multiparous holstein cows were assigned to 2 treatments in a randomized complete block design; cows in the direct-fed microbial treatm ... | 2015 | 26162791 |
| production of a cellulase-free alkaline xylanase from bacillus pumilus mtcc 5015 by submerged fermentation and its application in biobleaching. | here, we described the production of a cellulase-free alkaline xylanase from bacillus pumilus mtcc 5015 by submerged fermentation and its application in biobleaching. various process parameters affecting xylanase production by b. pumilus were optimized by adopting a plackett-burman design (pbd) as well as response surface methodology (rsm). these statistical methods aid in improving the enzyme yield by analysing the individual crucial components of the medium. maximum production was obtained wit ... | 2015 | 26155675 |
| synthesis and characterization of magnetite/silver/antibiotic nanocomposites for targeted antimicrobial therapy. | the article is devoted to preparation and characterization of magnetite/silver/antibiotic nanocomposites for targeted antimicrobial therapy. magnetite nanopowder was produced by thermochemical technique; silver was deposited on the magnetite nanoparticles in the form of silver clusters. magnetite/silver nanocomposite was investigated by xrd, sem, tem, afm, xps, edx techniques. adsorptivity of magnetite/silver nanocomposite towards seven antibiotics from five different groups was investigated. it ... | 2015 | 26117765 |
| synthesis and characterization of bioactive acylpyrazolone sulfanilamides and their transition metal complexes: single crystal structure of 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-one sulfanilamide. | two schiff base ligands ampp-sn 1 and bmpp-sn 2, afforded by a condensation reaction between sulfanilamide and the respective acylpyrazolone carbonyl precursors, their mn(ii), co(ii), ni(ii), and cu(ii) complexes prepared by the reaction of ligands and corresponding metal salts in aqueous solutions, were synthesized and then characterized by both analytical and spectroscopic methods, in a view to developing new improved bioactive materials with novel properties. on the basis of elemental analysi ... | 2015 | 26106285 |
| biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed in escherichia coli. | laccases are multi-copper oxidases that oxidize a broad range of substrates at the expense of molecular oxygen, without any need for co-factor regeneration. these enzymes bear high potential for the sustainable synthesis of fine chemicals and the modification of (bio)polymers. here we describe cloning and expression of five novel bacterial laccase-like multi copper oxidases (lmcos) of diverse origin which were identified by homology searches in online databases. activity yields under different e ... | 2015 | 26068013 |
| [identification of 2',3'-cgmp as an intermediate of rna catalytic cleavage by binase and evaluation of its biological action]. | binase--bacillus pumilus rnase--endonuclease cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-oh residue of adjacent nucleotides with the formation of corresponding intermediate 2',3'-cgmp. subsequent hydrolysis of 2',3'-cgmp into 3'-phosphate is highly specific and occurs slowly so the question arises about the existing time of that positional isomer during rna catalytic cleavage by binase and about 2',3'-cgmp role in antitumor activity of the enzyme: in present work b ... | 2015 | 26050470 |
| silver nanoparticles synthesis mediated by new isolates of bacillus spp., nanoparticle characterization and their activity against bean yellow mosaic virus and human pathogens. | extracellular agents produced by newly isolated bacterial strains were able to catalyze the synthesis of silver nanoparticles (agnps). the most effective isolates were identified as bacillus pumilus, b. persicus, and bacillus licheniformis using molecular identification. dls analysis revealed that the agnps synthesized by the above strains were in the size range of 77-92 nm. tem observations showed that the nanoparticles were coated with a capping agent, which was probably involved in nanopartic ... | 2015 | 26029190 |
| genomic analysis of a ginger pathogen bacillus pumilus providing the understanding to the pathogenesis and the novel control strategy. | bacillus pumilus has been widely identified as a pathogen of plant and human, while the genetic information is rarely available for pathogenic b. pumilus strains. b. pumilus gr8 is a pathogen that causes ginger rhizome rot disease by invading ginger rhizome parenchymatous tissues, growing in the extracellular space, and producing plant cell wall-degrading enzymes to destroy ginger cells. in this study, the genome of gr8 was sequenced and characterized. this genome was the third completely sequen ... | 2015 | 25989507 |
| reclassification of bacillus invictae as a later heterotypic synonym of bacillus altitudinis. | the aim of this study was to reclarify the taxonomic status of strain bacillus invictae bi.ffup1t by performing comparative analyses with the other four type strains within the bacillus pumilus group. the digital dna-dna hybridization (dddh) and average nucleotide identity (ani) values between strains b. invictae bi.ffup1t ( = dsmz 26896t = mccc 1a07089t), b. altitudinis 41kf2bt ( = dsmz 21631t = mccc 1a06452t), b. safensis fo-36bt ( = dsmz 19292t = mccc 1a6451t), b. pumilus atcc 7061t ( = dsmz ... | 2015 | 25977285 |
| inoculation of phaseolus vulgaris with the nodule-endophyte agrobacterium sp. 10c2 affects richness and structure of rhizosphere bacterial communities and enhances nodulation and growth. | agrobacterium sp. 10c2 is a nonpathogenic and non-symbiotic nodule-endophyte strain isolated from root nodules of phaseolus vulgaris. the effect of this strain on nodulation, plant growth and rhizosphere bacterial communities of p. vulgaris is investigated under seminatural conditions. inoculation with strain 10c2 induced an increase in nodule number (+54 %) and plant biomass (+16 %). grains also showed a significant increase in phosphorus (+53 %), polyphenols (+217 %), flavonoids (+62 %) and to ... | 2015 | 25967041 |
| salt stress represses production of extracellular proteases in bacillus pumilus. | bacillus pumilus is able to secrete subtilisin-like prote-ases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. in the current study, the biosynthesis and transcription of extracellular pro-teases in b. pumilus (ba06) under salt stress were investigated using various methods, including a proteolytic assay, zymogram analysis, and real-time pcr. our results showed that total extracellular proteolytic activity, both i ... | 2015 | 25966269 |
| complete genome sequence of bacillus pumilus w3: a strain exhibiting high laccase activity. | here we report the full genome sequence of bacillus pumilus w3, which was isolated from raw gallnut honey in nandan county, guangxi province of china, showing high cota-laccase activity. the w3 strain contains 3,745,123bp with gc content of 41.39%, and contains 3695 protein-coding genes, 21 rrnas and 70 trnas. | 2015 | 25957807 |
| assessment of microbial contamination within working environments of different types of composting plants. | the objective of the study was to determine the degree of microbiological contamination, type of microflora, bioaerosol particle size distribution, and concentration of endotoxins in dust in different types of composting plants. in addition, this study provides a list of indicator microorganisms that pose a biological threat in composting facilities, based on their prevalence within the workplace, source of isolation, and health hazards. we undertook microbiological analysis of the air, work sur ... | 2015 | 25947216 |
| isolation of potential probiotic bacillus spp. and assessment of their subcellular components to induce immune responses in labeo rohita against aeromonas hydrophila. | bacillus species isolated from the gut of healthy labeo rohita (hamilton) were screened for antibacterial activity against selected fish pathogens. among the isolates, kadr5 and kadr6 showed antibacterial activity, tolerated low ph and high bile concentrations and were susceptibility to various antibiotics. based on morphological and biochemical tests and 16s rrna gene analysis the probiotic strains kadr5 and kadr6 were identified as bacillus licheniformis and bacillus pumilus, respectively. the ... | 2015 | 25917974 |
| chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants. | crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ... | 2015 | 25910648 |
| sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide. | bacillus pumilus safr-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, uv radiation and chemical disinfection than other spore-forming bacteria. the survival of b. pumilus safr-032 spores to standard clean room sterilization practices requires development of more stringent disinfection agents. here, we report the effects of a stabilized chlorine dioxide-based biocidal agent against spores of b. pumilus safr-032 and bacillus subt ... | 2015 | 25897406 |
| [expression optimization of bacillus pumilus subtilisin-like proteinase]. | the characteristics of bacillus pumilus subtilisin-like protease expression were investigated. potential binding sites for the transcription factor degu~p have been identified in the regulatory region of the enzyme gene, which have been optimized for interaction with the regulatory protein. the expression of the extracellular subtilisin-like protease modified gene has been examined. it was established that the modification of one of the sites has resulted in increased expression of the proteinas ... | 2015 | 25895372 |
| isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of parangipettai coastal waters. | multidrug resistance of heterotrophic bacteria isolated from an aquaculture farm effluent in parangipettai, at the southeastern coast of india, was investigated. in the initial screening, 27 antibiotic-resistant strains were isolated. all the strains were tested for antibiotic susceptibility against chloramphenicol with varying concentrations. from these, two highly resistant strains, i.e. s1 and s5, were isolated. the selected strains were identified by 16s ribosomal rna (rrna) sequencing techn ... | 2015 | 25850744 |
| efficient secretory production of cota-laccase and its application in the decolorization and detoxification of industrial textile wastewater. | fungal laccases are typically unstable at high ph and temperature conditions, which limit their application in the decolorization of textile wastewater. by contrast, the highly stable bacterial laccases can function within a wider ph range and at high temperatures, thus have significant potential in treatment for textile wastewater. in our previous work, a thermo-alkali-stable cota-laccase gene was cloned from bacillus pumilus w3 and overexpressed in escherichia coli. in this study, the robust c ... | 2015 | 25847445 |
| growth and (137)cs uptake of four brassica species influenced by inoculation with a plant growth-promoting rhizobacterium bacillus pumilus in three contaminated farmlands in fukushima prefecture, japan. | the effectiveness of the plant growth-promoting rhizobacterium bacillus pumilus regarding growth promotion and radiocesium ((137)cs) uptake was evaluated in four brassica species grown on different (137)cs contaminated farmlands at fukushima prefecture in japan from june to august 2012. b. pumilus inoculation did not enhance growth in any of the plants, although it resulted in a significant increase of (137)cs concentration and higher (137)cs transfer from the soil to plants. the brassica specie ... | 2015 | 25847170 |
| a membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes. | a biocathode was designed by the modification of a carbon nanotube (cnt) gas-diffusion electrode with bilirubin oxidase from bacillus pumilus, achieving high current densities up to 3 ma cm(-2) for the reduction of o2 from air. a membraneless air-breathing hydrogen biofuel cell was designed by combination of this cathode with a functionalized cnt-based hydrogenase anode. | 2015 | 25845356 |
| the hyperaccumulator sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil. | endophyte-assisted phytoremediation has recently been suggested as a successful approach for ecological restoration of metal contaminated soils, however little information is available on the influence of endophytic bacteria on the phytoextraction capacity of metal hyperaccumulating plants in multi-metal polluted soils. the aims of our study were to isolate and characterize metal-resistant and 1-aminocyclopropane-1-carboxylate (acc) utilizing endophytic bacteria from tissues of the newly discove ... | 2015 | 25796039 |
| utilization of microbial community potential for removal of chlorpyrifos: a review. | chlorpyrifos (cp) is the most commonly used pesticide in agricultural fields worldwide. exposure to cp and its metabolites creates severe neuron-disorders in human beings. improper handling and uncontrolled application of cp by farmers have lead to the contamination of surface and ground water bodies. biodegradation offers an efficient and cost effective method for the removal of cp and other toxic organophosphorus pesticides from the contaminated environment. the degradation of cp by various mi ... | 2016 | 25782532 |
| draft genome sequences of five spore-forming food isolates of bacillus pumilus. | here, we report the draft genome sequences of five food isolates of bacillus pumilus, a spore-forming gram-positive bacterium. | 2015 | 25767247 |
| a novel intracellular nitrogen-fixing symbiosis made by ustilago maydis and bacillus spp. | we observed that the maize pathogenic fungus ustilago maydis grew in nitrogen (n)-free media at a rate similar to that observed in media containing ammonium nitrate, suggesting that it was able to fix atmospheric n2 . because only prokaryotic organisms have the capacity to reduce n2 , we entertained the possibility that u. maydis was associated with an intracellular bacterium. the presence of nitrogenase in the fungus was analyzed by acetylene reduction, and capacity to fix n2 by use of (15) n2 ... | 2015 | 25754368 |
| characterization of deep sea fish gut bacteria with antagonistic potential, from centroscyllium fabricii (deep sea shark). | the bacterial isolates from centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. this study focuses on strain btss-3, which showed antimicrobial activity against pathogenic bacteria including salmonella typhimurium, proteus vulgaris, clostridium perfringens, staphylococcus aureus, bacillus cereus, bacillus circulans, bacillus macerans and bacillus pumilus. btss3 was subjected to phenotypic characterization using bio ... | 2015 | 25740801 |
| isolation and characterisation of bacillus spp. antagonistic to vibrio parahaemolyticus for use as probiotics in aquaculture. | acute gastroenteritis caused by pathogenic vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. in this study, a total of 249 isolates were isolated from four types of seafood (litopenaeus vannamei, oratosquilla oratoria, mactra veneriformis and portunus trituberculatus) and ... | 2015 | 25737203 |
| microbiological bioassay using bacillus pumilus to detect tetracycline in milk. | the tetracyclines (tcs) are widely used in the treatment of several diseases of cattle and their residues may be present in milk. to control these residues it is necessary to have available inexpensive screening methods, user-friendly and capable of analysing a high number of samples. the purpose of this study was to design a bioassay of microbiological inhibition in microtiter plates with spores of bacillus pumilus to detect tcs at concentrations corresponding to the maximum residue limits (mrl ... | 2015 | 25720802 |
| heterologous expression of mitochondria-targeted microbial nitrilase enzymes increases cyanide tolerance in arabidopsis. | anthropogenic activities have resulted in cyanide (cn) contamination of both soil and water in many areas of the globe. while plants possess a detoxification pathway that serves to degrade endogenously generated cn, this system is readily overwhelmed, limiting the use of plants in bioremediation. genetic engineering of additional cn degradation pathways in plants is one potential strategy to increase their tolerance to cn. here we show that heterologous expression of microbial nitrilase enzymes ... | 2015 | 25711239 |
| isolation and identification of local bacillus isolates for xylanase biosynthesis. | bacillus species are attractive industrial organisms due to their rapid growth rates leading to a short fermentation cycle and for their capacity to secrete important enzymes and proteins such as xylanase into the extracellular medium. considering the industrial importance of xylanase, in this current study, bacillus spp. were isolated from different soils and were screened for their xylanase production. | 2014 | 25705364 |
| [structural properties and functional importance of metzincin metalloproteinases]. | here wediscuss known properties of metzincin metalloproteinases, their structure, physiological roles in the cell and potential medical uses. we also present results describing a novel extracellular metzincin metalloproteinase from bacillus pumilus with a unique combination of properties typical for both astacins and adamalysins. | 2015 | 25702412 |
| diversity of bacteria nesting the plant cover of north sinai deserts, egypt. | north sinai deserts were surveyed for the predominant plant cover and for the culturable bacteria nesting their roots and shoots. among 43 plant species reported, 13 are perennial (e.g. fagonia spp., pancratium spp.) and 30 annuals (e.g. bromus spp., erodium spp.). eleven species possessed rhizo-sheath, e.g. cyperus capitatus, panicum turgidum and trisetaria koelerioides. microbiological analyses demonstrated: the great diversity and richness of associated culturable bacteria, in particular nitr ... | 2012 | 25685397 |
| antibacterial metabolites and bacteriolytic enzymes produced by bacillus pumilus during bacteriolysis of arthrobacter citreus. | the marine isolate bacillus pumilus sbug 1800 is able to lyse living cells of arthrobacter citreus on solid media as well as pasteurized a. citreus cells in liquid mineral salt medium. the cultivation of b. pumilus in the presence of pasteurized a. citreus is accompanied by an enhanced production of 2,5-diketopiperazines (dkps). dkps inhibit bacterial growth, but do not seem to cause bacteriolysis. this study shows that b. pumilus also lyses living cells of a. citreus in co-culture experiments a ... | 2015 | 25678259 |
| unprecedented access of phenolic substrates to the heme active site of a catalase: substrate binding and peroxidase-like reactivity of bacillus pumilus catalase monitored by x-ray crystallography and epr spectroscopy. | heme-containing catalases and catalase-peroxidases catalyze the dismutation of hydrogen peroxide as their predominant catalytic activity, but in addition, individual enzymes support low levels of peroxidase and oxidase activities, produce superoxide, and activate isoniazid as an antitubercular drug. the recent report of a heme enzyme with catalase, peroxidase and penicillin oxidase activities in bacillus pumilus and its categorization as an unusual catalase-peroxidase led us to investigate the e ... | 2015 | 25663126 |
| isolation of haemolytic bacilli from field-collected culicoides oxystoma and culicoides peregrinus: potential vectors of bluetongue virus in west bengal, india. | two haemolytic bacterial strains of bacillus pumilus (cu1a, cu1b) and one blood-utilizing strain of bacillus licheniformis (cu2b) were isolated from relatively low numbers of field-collected females of culicoides oxystoma and culicoides peregrinus (diptera: ceratopogonidae). a total of 36 females, including 18 of each of c. oxystoma and c. peregrinus (consisting of one and a pool of eight blood-engorged specimens, and one and a pool of eight non-engorged specimens for each species), were tested. ... | 2015 | 25644315 |
| complete genome sequence of bacillus pumilus strain wp8, an efficient plant growth-promoting rhizobacterium. | bacillus pumilus strain wp8 is an efficient plant growth-promoting rhizobacterium. here, we present the complete genome of wp8 and its genes involved in plant growth promotion and biocontrol. | 2015 | 25614565 |
| corrigendum to "prevalence of thermoduric bacteria and spores on 10 midwest dairy farms". | thermoduric bacteria (tdb), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. they have the potential to adversely affect the quality and shelf life of products. the objectives of this study were to identify the origin and common species of heat-resistant bacteria occurring during summer and winter on midwest dairy farms. bulk tank milk samples were taken from 10 dairy farms located along the south dakota section of interstate 29, with ... | 2014 | 25610925 |
| evaluation of bacillus cereus and bacillus pumilus metabolites for anthelmintic activity. | to assess the anthelmintic acivity of bacillus cereus and bacillus pumilus metabolites. | 2017 | 25598639 |
| c-terminal hybrid mutant of bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and ph tolerance by reinforcing oligomerization. | to investigate the impact of the highly variable c-terminal domain of cyanide dihydratase, a member of the nitrilase superfamily, on its activity and stability. | 2015 | 25597384 |
| genomic and chemical insights into biosurfactant production by the mangrove-derived strain bacillus safensis ccma-560. | many bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on bacillus subtilis. surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. this study is aimed to unveil the biosurfactant metabolic ... | 2015 | 25586584 |
| bacillus species isolated from tungrymbai and bekang, naturally fermented soybean foods of india. | tungrymbai and bekang are naturally fermented soybean foods commonly consumed in meghalaya and mizoram states of india. a total of 39 samples of tungrymbai and 43 samples of bekang were collected from different villages and markets of meghalaya and mizoram, respectively and were analysed for microbial load. in both tungrymbai and bekang, the average population of bacillus spp. was 8.2±0.1 log cfu/g. a total of 428 isolates of bacillus were isolated from tungrymbai (211) and bekang (217) for deta ... | 2015 | 25574846 |
| increased mrna expression of selected pro-inflammatory factors in inflamed bovine endometrium in vivo as well as in endometrial epithelial cells exposed to bacillus pumilus in vitro. | endometrial epithelium plays a crucial role in the first immune response to invading bacteria by producing cytokines and chemokines. the aim of this study was to investigate the first inflammatory response of the endometrium in vivo and in vitro. gene expression of several pro-inflammatory factors and toll-like receptors (tlr2, -4, -6) was determined in endometrial cytobrush samples obtained from healthy cows and cows with clinical or subclinical endometritis. endometrial epithelial cells were c ... | 2015 | 25562589 |
| new insight into secreted ribonuclease structure: binase is a natural dimer. | the biological effects of ribonucleases (rnases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. one of the most intriguing properties of rnases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient rna hydrolysis and the evasion of ribonuclease inhibitor. dimeric forms of microbial rnase binase secreted by bacillus pumilus (former b. intermedius) have ... | 2014 | 25551440 |
| probing c-terminal interactions of the pseudomonas stutzeri cyanide-degrading cynd protein. | the cyanide dihydratases from bacillus pumilus and pseudomonas stutzeri share high amino acid sequence similarity throughout except for their highly divergent c-termini. however, deletion or exchange of the c-termini had different effects upon each enzyme. here we extended previous studies and investigated how the c-terminus affects the activity and stability of three nitrilases, the cyanide dihydratases from b. pumilus (cyndpum) and p. stutzeri (cyndstut) and the cyanide hydratase from neurospo ... | 2015 | 25549622 |
| halotolerant pgprs prevent major shifts in indigenous microbial community structure under salinity stress. | the resilience of soil microbial populations and processes to environmental perturbation is of increasing interest as alteration in rhizosphere microbial community dynamics impacts the combined functions of plant-microbe interactions. the present study was conducted to investigate the effect of inoculation with halotolerant rhizobacteria bacillus pumilus (str2), halomonas desiderata (str8), and exiguobacterium oxidotolerans (str36) on the indigenous root-associated microbial (bacterial and funga ... | 2015 | 25542205 |