Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| bacteroides plebeius sp. nov. and bacteroides coprocola sp. nov., isolated from human faeces. | nine strains of gram-negative, anaerobic rod were isolated from human faeces. based on phylogenetic analysis and specific phenotypic characteristics, these strains were included within the bacteroides cluster and were divided into two clusters. strains from the two clusters showed 16s rrna gene sequence similarities of 90.4 and 92.7% to the nearest recognized species, bacteroides vulgatus. the strains also formed two clusters exhibiting a 16s rrna gene sequence divergence of approximately 6%. dn ... | 2005 | 16166722 |
| trypanosoma cruzi: influence of predominant bacteria from indigenous digestive microbiota on experimental infection in mice. | to verify the influence of some predominant components from indigenous microbiota on systemic immunological responses during experimental chagas disease, germ-free nih swiss mice were mono-associated with escherichia coli, enterococcus faecalis, bacteroides vulgatus or peptostreptococcus sp. and then infected with the y strain of trypanosoma cruzi. all the mono-associations predominantly induced a th1 type of specific immune response to the infection by t. cruzi. a direct correlation was observe ... | 2005 | 16038901 |
| innate mechanisms for bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats. | bifidobacteria comprise a dominant microbial population group in the human intestinal tract with purported beneficial health effects on the host. in this study, we characterized the molecular mechanisms for the initial interaction of probiotic bifidobacterium lactis strain bb12 with native and intestinal epithelial cell (iec) lines. we showed that b. lactis-monoassociated fisher f344 rats transiently induce phosphorylation/activation of the nf-kappab transcriptionally active subunit rela and the ... | 2005 | 16011513 |
| in vitro activity of tigecycline against bacteroides species. | to ascertain the current susceptibility patterns of members of the bacteroides fragilis group in our hospital and to assess the in vitro activity of tigecycline against these organisms. | 2005 | 15951353 |
| association with selected bacteria does not cause enterocolitis in il-10 gene-deficient mice despite a systemic immune response. | resident bacteria have been implicated to play a major role in the development of inflammatory bowel disease. while luminally sterile il-10 gene-deficient mice remain disease-free, their conventionally raised littermates develop enterocolitis associated with increased numbers of luminal and mucosal adherent bacteria. to investigate the role of defined bacteria on the initiation and development of this enterocolitis, we associated luminally sterile il-10 gene-deficient mice with pure strains of r ... | 2005 | 15906767 |
| bacteroides massiliensis sp. nov., isolated from blood culture of a newborn. | gram-negative anaerobic rods were isolated from blood culture of a healthy baby born at term. based on phylogenetic analysis and specific phenotypic characteristics, this strain was included within the bacteroides cluster. the novel bile-resistant anaerobic species was designated bacteroides massiliensis and it has 92.8 % 16s rrna similarity to bacteroides vulgatus and a dna g + c content of 49 mol%. the major cellular fatty acid is anteiso-branched c(15 : 0). the type strain of b. massiliensis ... | 2005 | 15879278 |
| genetic and structural analysis of the bacteroides conjugative transposon ctn341. | the genetic structure and functional organization of a bacteroides conjugative transposon (ctn), ctn341, were determined. ctn341 was originally isolated from a tetracycline-resistant clinical isolate of bacteroides vulgatus. the element was 51,993 bp long, which included a 5-bp coupling sequence that linked the transposon ends in the circular form. there were 46 genes, and the corresponding gene products fell into three major functional groups: dna metabolism, regulation and antibiotic resistanc ... | 2005 | 15805532 |
| characterization of plasmid por1 from ornithobacterium rhinotracheale and construction of a shuttle plasmid. | the bacterium ornithobacterium rhinotracheale has been recognized as an emerging pathogen in poultry since about 10 years ago. knowledge of this bacterium and its mechanisms of virulence is still very limited. here we report the development of a transformation system that enables genetic modification of o. rhinotracheale. the system is based on a cryptic plasmid, por1, that was derived from an o. rhinotracheale strain of serotype k. sequencing indicated that the plasmid consisted of 14,787 nucle ... | 2004 | 15466524 |
| 15-deoxy-delta12,14-prostaglandin j2-mediated erk signaling inhibits gram-negative bacteria-induced rela phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2a activity. | we have previously shown that non-pathogenic gram-negative bacteroides vulgatus induces transient rela phosphorylation (ser-536), nf-kappab activity, and pro-inflammatory gene expression in native and intestinal epithelial cell (iec) lines. we now demonstrate that 15-deoxy-delta(12,14)-prostaglandin j(2) (15d-pgj(2)) but not prostaglandin e(2) inhibits lipopolysaccharide (lps) (b. vulgatus)/lps (escherichia coli)-induced rela phosphorylation and interleukin-6 gene expression in the colonic epith ... | 2004 | 15199053 |
| differential effect of immune cells on non-pathogenic gram-negative bacteria-induced nuclear factor-kappab activation and pro-inflammatory gene expression in intestinal epithelial cells. | we have previously shown that non-pathogenic gram negative bacteria induce rela phosphorylation, nuclear factor (nf)-kappab transcriptional activity and pro-inflammatory gene expression in intestinal epithelial cells (iec) in vivo and in vitro. in this study, we investigated the molecular mechanism of immune-epithelial cell cross-talk on gram-negative enteric bacteria-induced nf-kappab signalling and pro-inflammatory gene expression in iec using ht-29/mtx as well as caco-2 transwell cultures int ... | 2004 | 15147574 |
| dna from probiotic bacteria modulates murine and human epithelial and immune function. | the intestinal epithelium must discriminate between pathogenic and nonpathogenic bacteria and respond accordingly. the aim of this study was to examine whether bacterial dna can serve as the molecular basis for bacterial recognition. | 2004 | 15131797 |
| commensal gram-negative bacteria prime human dendritic cells for enhanced il-23 and il-27 expression and enhanced th1 development. | dendritic cells (dc) are the main orchestrators of specific immune responses. depending on microbial information they encounter in peripheral tissues, they promote the development of th1, th2 or unpolarized th cell responses. in this study we have investigated the immunomodulatory effect of non-pathogenic intestinal gram-negative (escherichia coli, bacteroides vulgatus,veillonella parvula, pseudomonas aeruginosa) and gram-positive (bifidobacterium adolescentis, enterococcus faecalis, lactobacill ... | 2004 | 15114670 |
| experimental swine dysentery: comparison between infection models. | the aim of the present study was to develop a reproducible porcine infection model with brachyspira hyodysenteriae. the influence of different factors was evaluated, namely, age, a diet containing large quantities of soybean meal, housing and administration of cortisol or antacids. furthermore, the synergistic effect of additional bacteria (escherichia coli o141, bacteroides vulgatus or a mixture of bacteroides fragilis, a field isolate of bacteroides and fusobacterium necrophorum) was studied. ... | 2004 | 15017282 |
| lipopolysaccharide induces cd25-positive, il-10-producing lymphocytes without secretion of proinflammatory cytokines in the human colon: low md-2 mrna expression in colonic macrophages. | despite the huge number of colonized gram-negative bacteria in the colon, the normal colon maintains its homeostasis without any excessive immune response. to investigate the potential mechanisms involved, human colonic lamina propria mononuclear cells (lpmcs) obtained from uninflamed mucosa were cultured with lipopolysaccharide (lps) prepared from bacteroides vulgatus (bv-lps) or bacteroides fragilis (bf-lps), as representatives of indigenous flora, or pathogenic salmonella minnesota (sm-lps). ... | 2004 | 14997033 |
| genetic analysis of an ambler class a extended-spectrum beta-lactamase from capnocytophaga ochracea. | a beta-lactamase gene (cfxa3, 966 bp) was isolated from a beta-lactam-resistant capnocytophaga ochracea clinical isolate and amplified using primers from the cfxa gene of bacteroides vulgatus. the mics of third-generation cephalosporins were much higher than those of the transconjugant escherichia coli strain. the deduced protein sequence, by comparison with cfxa2 of prevotella intermedia, had a y239d substitution and possessed the characteristics of a class a, group 2e beta-lactamase. | 2004 | 14766881 |
| culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. | renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ... | 2003 | 14662931 |
| the suppressive effect of bifidobacteria on bacteroides vulgatus, a putative pathogenic microbe in inflammatory bowel disease. | bacteroides, a predominant commensal bacteria in the gut, are thought to be responsible for the development of inflammatory bowel disease (ibd). in the present study, we examined whether or not bifidobacteria suppress b. vulgatus, a representative pathogenic bacteroides species, in both the coculture system and the gnotobiotic murine model. as a result, bifidobacterium infantis 1222 highly inhibited the growth of b. vulgatus in the coculture and also significantly suppressed the systemic antibod ... | 2003 | 12906096 |
| accelerated alveolar bone loss in mice lacking interleukin-10. | interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. we hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. male interleukin-10(-/-) mice, on 129/svev and c57bl/6j background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. interleukin-10 ... | 2003 | 12885849 |
| emergence of fluoroquinolone resistance among bacteroides species. | several newer generation fluoroquinolones have demonstrated good in vitro activity against bacteroides species; particularly when first introduced. however, resistance of bacteroides to quinolones appears to be increasing. | 2003 | 12865399 |
| bacteroides vulgatus protects against escherichia coli-induced colitis in gnotobiotic interleukin-2-deficient mice. | the microflora plays a crucial role in inflammatory bowel diseases (ibds). specific pathogen-free (spf), but not germ-free, interleukin (il)-2-deficient (il-2-/-) mice develop colitis. the colitogenicity of commensal bacteria was determined. | 2003 | 12851881 |
| [intestinal flora and crohn's disease]. | the pathogenesis of inflammatory bowel diseases (ibd) proceeds through stages of initiation, amplification and healing. abundant clinical and experimental data incriminate luminal bacteria or bacterial products in both the initiation and perpetuation of chronic intestinal inflammation. macrophage and t-cell activation with accompanying inflammatory cytokine production appears to be an early event. studies of lymphocyte responsiveness to autologous and heterologous intestinal bacteria have sugges ... | 2003 | 12843962 |
| fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats. | the effects of fructans in the diet on the mucosal morphometry (height of villi, depth of the crypts, number of goblet cells), the thickness of the epithelial mucus layer and the histochemical composition of intestinal mucosubstances in the distal jejunum and the distal colon were investigated by comparing germ-free (gf) rats, rats harbouring bacteroides vulgatus and bifidobacterium longum (diassociated (da) rats), and rats with a human faecal flora (hfa). the rats were fed either a commercial s ... | 2003 | 12720580 |
| sequence analysis of cfxa2-like beta-lactamases in prevotella species. | sixty-two strains of oral (32) and non-oral (30) prevotella producing beta-lactamases were screened for cfxa by pcr, using an intragenic primer pair. all 62 were cfxa/cfxa2 positive. fourteen of these strains, representing seven pigmented and seven non-pigmented prevotella species were submitted to further pcr with specific primers that amplified the whole beta-lactamase structural gene (966 bp). after cloning and sequencing, the deduced amino acid sequences were compared with that of bacteroide ... | 2003 | 12697645 |
| transforming growth factor-beta 1 inhibits non-pathogenic gram negative bacteria-induced nf-kappa b recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation. | we have shown that non-pathogenic enteric gram-negative bacteroides vulgatus induces rela phosphorylation, nf-kappab activation, and proinflammatory gene expression in primary and intestinal epithelial cell (iec) lines. we now demonstrate the transient induction of nuclear phospho-rela (day 3) followed by persistent activation of phospho-smad2 (days 3 and 7) in iec from mucosal tissue sections of b. vulgatus-monoassociated rats, indicating that both nf-kappab and transforming growth factor-beta1 ... | 2003 | 12672795 |
| prevention of gut inflammation by bifidobacterium in dextran sulfate-treated gnotobiotic mice associated with bacteroides strains isolated from ulcerative colitis patients. | indigenous bacteroides strains are closely associated with the occurrence and exacerbation of ulcerative colitis (uc). in this study, we aimed to clarify the effect of bifidobacterium strains, another major member of colonic bacteria, on the development of gut inflammation using gnotobiotic mouse models associated with bacteroides strains isolated from uc patients. dextran sulfate (dss) administration induced inflammation in the large intestine, in particular of the cecum, in the gnotobiotic mic ... | 2003 | 12650769 |
| activities of garenoxacin (bms-284756) and other agents against anaerobic clinical isolates. | a total of 590 clinical isolates consisting of 33 species of both gram-positive and gram-negative anaerobes were collected from nine centers in the chicago area in 1998-1999. the largest number of isolates (330 isolates, 56%) belonged to the bacteroides group. isolates were tested by agar dilution against garenoxacin (bms-284756, t-3811 me), trovafloxacin, moxifloxacin, clindamycin, imipenem, piperacillin-tazobactam, and cefoxitin. all but one species (2% of bacteroides vulgatus isolates) were f ... | 2003 | 12604521 |
| lactobacillus gg prevents recurrence of colitis in hla-b27 transgenic rats after antibiotic treatment. | bacteroides vulgatus induces colitis in gnotobiotic hla-b27 transgenic (tg) rats while broad spectrum antibiotics prevent and treat colitis in specific pathogen free (spf) tg rats although disease recurs after treatment ends. lactobacilli treat human pouchitis and experimental colitis. we investigated if lactobacillus rhamnosus gg (l gg) can prevent colitis in tg rats monoassociated with b vulgatus and if l gg or lactobacillus plantarum 299v (lp 299v) can treat established colitis in spf tg rats ... | 2003 | 12584218 |
| role of commensal bacteria in chronic experimental colitis: lessons from the hla-b27 transgenic rat. | rats on lewis or fischer background, transgenic for human hla-b27 and beta(2)-microglobulin genes spontaneously develop colitis, gastritis, arthritis, dermatitis, orchitis, epididymitis, carditis, alopecia and nail changes. disease susceptibility correlates with the gene copy number and is influenced by the genetic background. the pathomechanism in this model is still not completely understood. cell transfer experiments indicate an essential role of hla-b27 expression in bone marrow-derived cell ... | 2002 | 12571416 |
| comparison of the faecal microflora of patients with ankylosing spondylitis and controls using molecular methods of analysis. | to determine whether differences within the complex intestinal microflora can be demonstrated between patients with ankylosing spondylitis (as) and healthy individuals. | 2002 | 12468819 |
| quantification of bacteria adherent to gastrointestinal mucosa by real-time pcr. | the use of real-time quantitative pcr (5' nuclease pcr assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. we developed primers and probes based on the 16s ribosomal dna gene sequences for the detection of escherichia coli and bacteroides vulgatus. dna was isolated from pure cultures and from gut biopsy specimens and quantified by the 5' nuclease pcr assay. the assay showed a very high sensitivity: as little as 1 cfu of e. coli and 9 cfu of ... | 2002 | 12454130 |
| innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. | the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ... | 2002 | 12438343 |
| in vitro activities of newer quinolones against bacteroides group organisms. | the activities of bms-284576, clinafloxacin, moxifloxacin, sitafloxacin, trovafloxacin, imipenem, cefoxitin, and clindamycin against 589 bacteroides fragilis group isolates were determined. the activity of bms-284576 was comparable to that of trovafloxacin. sitafloxacin and clinafloxacin were the most active quinolones, and moxifloxacin was the least active. b. fragilis was the most susceptible of the species, and bacteroides vulgatus was the most resistant. association of specific antibiotic re ... | 2002 | 12234859 |
| national survey on the susceptibility of bacteroides fragilis group: report and analysis of trends for 1997-2000. | the results of a multicenter us survey using the national committee for clinical laboratory standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of bacteroides fragilis group species were compared from 1997 to 2000. the test panel consisted of 14 antibiotics: 3 carbapenems, 3 beta-lactam-beta-lactamase inhibitors, 3 cephamycins, 2 fluoroquinolones, clindamycin, chloramphenicol, and metronidazole. declines in the geometric mean minimum inhibitory conc ... | 2002 | 12173121 |
| design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples. | an oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ... | 2002 | 12167534 |
| quantitative analysis of bacterial dna from mycobacteria spp., bacteroides vulgatus, and escherichia coli in tissue samples from patients with inflammatory bowel diseases. | the etiology of inflammatory bowel diseases is unknown. mycobacteria spp., bacteroides vulgatus, and escherichia coli have been suspected to be involved. the aim of the present study was to examine the possible relationship between inflammatory bowel diseases and these microbes. | 2002 | 12162408 |
| structural study on lipid a and the o-specific polysaccharide of the lipopolysaccharide from a clinical isolate of bacteroides vulgatus from a patient with crohn's disease. | bacteroides vulgatus has been shown to be involved in the aggravation of colitis. previously, we separated two potent virulence factors, capsular polysaccharide (cps) and lipopolysaccharide (lps), from a clinical isolate of b. vulgatus and characterized the structure of cps. in this study, we elucidated the structures of o-antigen polysaccharide (ops) and lipid a in the lps. lps was subjected to weak acid hydrolysis to produce the lipid a fraction and polysaccharide fraction. lipid a was isolate ... | 2002 | 12153568 |
| ikk beta and phosphatidylinositol 3-kinase/akt participate in non-pathogenic gram-negative enteric bacteria-induced rela phosphorylation and nf-kappa b activation in both primary and intestinal epithelial cell lines. | pathogenic and enteroinvasive bacteria have been shown to trigger the i kappa b/nf-kappa b transcriptional system and proinflammatory gene expression in epithelial cells. in this study, we investigated the molecular mechanism of the commensal gram-negative bacteroides vulgatus-induced nf-kappa b signal transduction in intestinal epithelial cells (iec). we report that b. vulgatus induced interleukin-1 receptor-associated kinase-1 degradation, i kappa b alpha phosphorylation/degradation, rela and ... | 2002 | 12140289 |
| an oral introduction of intestinal bacteria prevents the development of a long-term th2-skewed immunological memory induced by neonatal antibiotic treatment in mice. | recent epidemiological studies indicate that antibiotic use in infancy may be associated with an increased risk of developing atopy. our previous work on animals demonstrated that kanamycin use during infancy promotes a shift in the th1/th2 balance towards a th2-dominant immunity. | 2002 | 12100062 |
| accelerated alveolar bone loss in hla-b27 transgenic rats: an adult onset condition. | patients with arthritis and crohn's disease may be more susceptible to periodontitis associated alveolar bone loss (abl). hla-b27 transgenic (tg) rats spontaneously develop arthritis and colitis. based on the hypothesis that tg rats would also be susceptible to abl, we compared the naturally occurring abl in tg and fischer 344 wild-type (wt) rats. | 2002 | 12064843 |
| adhesive property of bifidobacterium lactis lkm512 and predominant bacteria of intestinal microflora to human intestinal mucin. | the adhesive property to the intestinal mucin of bifidobacterium lactis lkm512, b. longum, b. breve, b. bifidum, b. adolescentis, b. infantis, bacteroides vulgatus, bacteroides distasonis, eubacterium aerofaciens, clostridium perfringens, escherichia coli, and lactobacillus acidophilus were examined. adhesive rate of lkm512 to the mucin was significantly (p < 0.05, 0.01, or 0.001) stronger than the other strains from 2 to 100 time. though the adhesive property of many strains was almost same to ... | 2002 | 11821930 |
| production and application of new monoclonal antibodies specific for a fecal helicobacter pylori antigen. | the aim of the present study was to establish monoclonal antibodies that could be used to produce a diagnostic test composed of one kind of monoclonal antibody recognizing a fecal helicobacter pylori antigen. the need to develop such a test arose from disadvantages of the diagnostic test that uses a polyclonal antibody or plural kinds of monoclonal antibodies, such as the lower specificity for h. pylori antigen and the difficulty of reproduction with consistent quality. mice were immunized with ... | 2002 | 11777832 |
| cloning and biochemical characterization of a class a beta-lactamase from prevotella intermedia. | the gene encoding a beta-lactamase of prevotella intermedia was cloned and sequenced. this gene, called cfxa2, shared 98% identity with cfxa, the structural gene of a beta-lactamase previously described in bacteroides vulgatus. the deduced protein sequence had a k272e substitution. cfxa2 had the characteristics of class a, group 2e beta-lactamases. | 2001 | 11451705 |
| gyra mutations associated with quinolone resistance in bacteroides fragilis group strains. | mutations in the gyra gene contribute considerably to quinolone resistance in escherichia coli. mechanisms for quinolone resistance in anaerobic bacteria are less well studied. the bacteroides fragilis group are the anaerobic organisms most frequently isolated from patients with bacteremia and intraabdominal infections. forty-four clinafloxacin-resistant and-susceptible fecal and clinical isolates of the b. fragilis group (eight bacteroides fragilis, three bacteroides ovatus, five bacteroides th ... | 2001 | 11408211 |
| sequence analysis of the plasmid prri2 from the rumen bacterium prevotella ruminicola 223/m2/7 and the use of prri2 in prevotella/bacteroides shuttle vectors. | prri2 is a small cryptic plasmid from the rumen bacterium prevotella ruminicola 223/m2/7 which has been used for the construction of shuttle vectors (prh3 and prri207) that replicate in many bacteroides/prevotella strains as well as in escherichia coli. sequence analysis of prri2 reveals that it is a 3240-bp plasmid carrying two clear open reading frames. rep, encoded by orf1, shows 48 and 47% amino acid sequence identity with repa proteins from bacteroides vulgatus and bacteroides fragilis, res ... | 2001 | 11407918 |
| structural elucidation of a capsular polysaccharide from a clinical isolate of bacteroides vulgatus from a patient with crohn's disease. | the structure of a capsular polysaccharide (cps) from a clinical isolate of bacteroides vulgatus was elucidated. b. vulgatus imcj 1204 was isolated from feces of a patient with crohn's disease. cps was prepared by phenol/water extraction of the bacterial cells followed by hydrophobic interaction chromatography and then gel filtration chromatography of the extract. the structure of cps was determined by chemical analysis and nmr spectroscopy including dqf-cosy, tocsy, roesy, hsqc-tocsy, hmqc and ... | 2001 | 11389714 |
| multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of bacteroides fragilis group, prevotella, fusobacterium, porphyromonas, and peptostreptococcus species. | in vitro surveys of antimicrobial resistance among clinically important anaerobes are an important source of information that can be used for clinical decisions in the choice of empiric antimicrobial therapy. this study surveyed the susceptibilities of 556 clinical anaerobic isolates from four large medical centers using a broth microdilution method. piperacillin-tazobactam was the only antimicrobial agent to which all the isolates were susceptible. similarly, imipenem, meropenem, and metronidaz ... | 2001 | 11257040 |
| comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria. | the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ... | 2001 | 11120995 |
| alteration of v beta usage and cytokine production of cd4+ tcr beta beta homodimer t cells by elimination of bacteroides vulgatus prevents colitis in tcr alpha-chain-deficient mice. | a major pathogenic factor for the development of inflammatory bowel disease (ibd) is the breakdown of the intestinal homeostasis between the host immune system and the luminal microenvironment. to assess the potential influence of luminal ags on the development of ibd, we fed tcr alpha(-/-) mice an elemental diet (ed). ed-fed tcr alpha(-/-) mice showed no pathologic features of ibd, and their aberrant mucosal b cell responses were suppressed. similar numbers of cd4(+), tcr betabeta homodimer t c ... | 2000 | 11067950 |
| preparation and preclinical evaluation of a novel liposomal complete-core lipopolysaccharide vaccine. | our objective is to develop a prophylactic vaccine strategy that can be evaluated for surgical and other high-risk hospitalized patients. in this paper, we describe the preparation and preclinical evaluation of a liposomal complete-core lipopolysaccharide (lps) vaccine that is nontoxic and broadly antigenic. complete-core (ra-chemotype) lpss were isolated from four gram-negative bacterial strains (escherichia coli k-12, e. coli r1, pseudomonas aeruginosa pac608, and bacteroides fragilis), mixed ... | 2000 | 11035726 |
| medical treatment of cholangiohepatitis and cholelithiasis in mature horses: 9 cases (1991-1998). | the medical approach to treatment of cholangiohepatitis and cholelithiasis in 9 horses is described. seven horses were treated successfully and returned to normal use, with a minimum follow-up period of 12 months. long-term antimicrobial therapy was believed to be critical in those cases that survived, with a median treatment duration of 51 days (range 17-124 days). treatment failure was associated with severe periportal and bridging hepatic fibrosis from biopsy material obtained at admission in ... | 2000 | 10952378 |
| in vitro activity of telithromycin (hmr 3647) and seven other antimicrobial agents against anaerobic bacteria. | we assessed the in vitro activity of telithromycin (hmr 3647) and seven other antimicrobials against 292 strains of obligately anaerobic bacteria. mics were determined with the microdilution technique and wilkins-chalgren broth according to din 58940-83. mic50/mic90s (mg/l) for telithromycin were 4/4 for bacteroides fragilis, bacteroides ovatus and bacteroides thetaiotaomicron, 2/4 for fusobacterium spp. and bilophila wadsworthia, 2/2 for bacteroides caccae, 1/4 for bacteroides vulgatus, 0.25/4 ... | 2000 | 10882699 |
| comparative activity of moxifloxacin in vitro against obligately anaerobic bacteria. | the antimicrobial activity of moxifloxacin and seven other antibiotics (four of them quinolones) against 292 strains of obligately anaerobic bacteria was assessed employing a broth microdilution technique performed in wilkens-chalgren broth. mic50/mic90 values (mg/l) for moxifloxacin were as follows: bacteroides fragilis (n = 62) 0.25/2, bacteroides ovatus (n = 70) 1/4, bacteroides vulgatus (n = 29) 0.25/1, bacteroides thetaiotaomicron (n = 17) 2/2, bacteroides caccae (n = 11) 1/2, prevotella sp ... | 2000 | 10795599 |
| characterization of antibody responses against rectal mucosa-associated bacterial flora in patients with ulcerative colitis. | previous reports on faecal microflora have demonstrated that the total number of aerobes and coliforms was increased in patients with ulcerative colitis (uc). based on the hypothesis that the pathogenesis of uc may be closely associated with the mucosal microflora, we investigated alterations in the mucosa-associated microflora of uc patients. | 2000 | 10719749 |
| microbes involved in dissimilatory nitrate reduction in the human large intestine. | nitrate-limited batch cultures, incorporating 20 different fermentation substrates and inoculated with human faeces, mainly selected for the growth of enterobacteria. the microbial diversity involved was determined by a combination of phenotypic and genotypic procedures. continuous culture with lactate as the sole electron donor selected for similar micro-organisms, but when antibiotics were incorporated to inhibit escherichia coli and lactate was replaced with choline, there was a wider microbi ... | 2000 | 10620715 |
| in vitro activity of gemifloxacin (sb 265805) against anaerobes. | gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ... | 1999 | 10471570 |
| differential induction of colitis and gastritis in hla-b27 transgenic rats selectively colonized with bacteroides vulgatus or escherichia coli. | resident bacteria play an important role in initiating and perpetuating gastrointestinal inflammation. we previously demonstrated that six commensal bacteria including bacteroides vulgatus caused more aggressive colitis and gastritis in hla-b27 transgenic rats than did the other five bacteria without b. vulgatus. this study compared the degree of gastrointestinal inflammation in gnotobiotic hla-b27 transgenic rats monoassociated with either b. vulgatus or escherichia coli. gnotobiotic transgenic ... | 1999 | 10338507 |
| dermoid cyst with dermal sinus tract complicated with spinal subdural abscess. | spinal subdural abscess caused by spread of infection with the dermal sinus tract is rare in children. this article reports on a 1-year-old male with prolonged fever, progressive paraplegia, and bowel and bladder dysfunction resulting from a spinal subdural abscess secondary to an infected spinal dermoid cyst with a dermal sinus tract. this is the youngest patient to be reported having this condition. surgical intervention was performed to find a tumor that had capsule and keratinlike contents. ... | 1999 | 10082349 |
| deconjugation ability of bacteria isolated from the jejunal fluid of patients with progressive systemic sclerosis and its gastric ph. | our goal was to demonstrate the role of bacteria in altered bile acid metabolism, which overgrow in the upper small intestine of patients with progressive systemic sclerosis. we identified the bacterial species, isolated from the jejunal fluid obtained from patients with progressive systemic sclerosis, who had previously shown an increase in 14co2, specific activity on breath test, and normal controls. after which, we investigated the deconjugation ability of the isolated bacteria and the relati ... | 1998 | 9840121 |
| resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. | mice with targeted deletion of the gene for interleukin-10 (il-10) spontaneously develop enterocolitis when maintained in conventional conditions but develop only colitis when kept in specific-pathogen-free (spf) environments. this study tested the hypothesis that enteric bacteria are necessary for the development of spontaneous colitis and immune system activation in il-10-deficient mice. il-10-deficient mice were maintained in either spf conditions or germfree conditions or were populated with ... | 1998 | 9784526 |
| immunochemical characterization of bacteroides vulgatus cell-surface antigens. | capsular polysaccharide (cps) and lipopolysaccharide (lps) antigens extracted from three bacteroides vulgatus strains (serotype specific strain, and two others isolated from normal gut flora and gut of a diabetic patient) were studied. chemical analysis of cps and purified lps antigens was performed. the compositions of sugars, amino acids and fatty acids were shown to be different for each antigen. rabbit antisera against whole bacterial cells were prepared. all antigens proved to be active in ... | 1998 | 9691430 |
| omeprazole induces altered bile acid metabolism. | it has been reported that the acidity of gastric contents could be an important factor in regulating jejunal flora. | 1998 | 9536953 |
| distribution and mobility of the tetracycline resistance determinant tetq. | we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ... | 1997 | 9372425 |
| antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects. | tosufloxacin, a quinolone, showed a broad antibacterial spectrum against gram-positive and gram-negative bacteria including anaerobic bacteria. tosufloxacin was 4- to 8-fold more active than levofloxacin and ciprofloxacin. the mic90 of tosufloxacin for clinical isolates of bacteroides fragilis, bacteroides vulgatus, bacteroides thetaiotaomicron and peptostreptococcus asaccharolyticus were 0.78, 0.39, 1.56 and 0.39 micrograms/ml, respectively. morphological observation with the scanning and trans ... | 1997 | 9142454 |
| conjugal transfer of the 5-nitroimidazole resistance plasmid pip417 from bacteroides vulgatus bv-17: characterization and nucleotide sequence analysis of the mobilization region. | three small 5-nitroimidazole (5-ni) resistance plasmids (pip417, pip419, and pip421) from bacteroides clinical isolates are transferable by a conjugative process during homologous or heterologous matings. the mobilization properties of pip417 originated from strain bv-17 of bacteroides vulgatus were studied. the plasmid was successfully introduced by in vitro conjugation into different strains of bacteroides and prevotella species and could be transferred back from these various strains to a pla ... | 1996 | 8955281 |
| alteration of bile acid metabolism by cimetidine in healthy humans. | to clarify an effect of cimetidine on bile acid metabolism, we evaluated whether an increased deconjugation of bile acids would occur in healthy humans who have received cimetidine. we examined: 1) whether healthy volunteers taking cimetidine would have positive bile acid breath tests because of bacterial overgrowth in the jejunum; 2) whether the isolated bacteria would exhibit deconjugation ability; and 3) whether a change in gastric ph was related to the bacterial overgrowth. | 1996 | 8952227 |
| pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. | pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ... | 1996 | 8919784 |
| the in vitro activity of trospectomycin against anaerobic bacteria. | the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ... | 1995 | 8667035 |
| selective enumeration of bacteroides vulgatus and b. distasonis organisms in the predominant human fecal flora by using monoclonal antibodies. | the genus bacteroides represents about one-third of the isolates from human fecal samples. the proportions of the different species are difficult to estimate because there is no method for rapid identification of mixtures of anaerobes. monoclonal antibodies against bacteroides vulgatus and b. distasonis were prepared. they did not react with the other bacteroides species of the b. fragilis group. these reagents allowed direct enumeration of b. vulgatus and b. diastasonis organisms in human fecal ... | 1996 | 8593077 |
| the pathogenic role of bacteroides vulgatus in patients with ulcerative colitis. | to clarify the role played by bacteroides vulgatus in the pathogenesis of ulcerative colitis, the serum antibody titer to the outer membrane protein of b. vulgatus, obtained from the inflamed rectal mucosa, and measured by enzyme-linked immunosorbent assay, was investigated in patients with ulcerative colitis. high serum antibody titers to the outer membrane protein of b. vulgatus were found in these patients. the specific outer membrane protein of b. vulgatus reactive to serum antibody was reco ... | 1995 | 8563888 |
| genetic analysis of the minimal replicon of plasmid pip417 and comparison with the other encoding 5-nitroimidazole resistance plasmids from bacteroides spp. | the nucleotide sequence of the dna replication origin region of a bacteroides vulgatus plasmid, pip417, encoding 5-nitroimidazole resistance has been determined. this region of 1934 bp presents some characteristics similar to those of other replication protein-dependent origins. it contains a large open reading frame which could encode a basic rep protein (repa) of 36.8 kda. upstream of this orf exist an at-rich region, three direct repeats (iterons) of 21 bp, multiple dnaa binding sites, and si ... | 1995 | 8559801 |
| genetic and biochemical analysis of a novel ambler class a beta-lactamase responsible for cefoxitin resistance in bacteroides species. | a clinical isolate of bacteroides vulgatus was resistant to tetracycline, clindamycin, ampicillin, cephaloridine, cefoxitin, and other beta-lactam antibiotics except imipenem. beta-lactam resistance was mediated by a membrane-associated, clavulanate-sensitive cephalosporinase capable of degrading cephalosporins and penicillins. cefoxitin also was degraded but at a slow rate. the cefoxitin resistance (fxr) determinant was cloned from b. vulgatus genomic libraries that were prepared in escherichia ... | 1993 | 8517690 |
| characterization of the mobilization region of a bacteroides insertion element (nbu1) that is excised and transferred by bacteroides conjugative transposons. | many bacteroides clinical isolates carry large conjugative transposons that, in addition to transferring themselves, excise, circularize, and transfer smaller, unlinked chromosomal dna segments called nbus (nonreplicating bacteroides units). we report the localization and dna sequence of a region of one of the nbus, nbu1, that was necessary and sufficient for mobilization by bacteroides conjugative transposons and by incp plasmids. the fact that the mobilization region was internal to nbu1 indic ... | 1993 | 8407836 |
| rape-seed meal toxicity in gnotobiotic rats: influence of a whole human faecal flora or single human strains of escherichia coli and bacteroides vulgatus. | gnotobiotic growing rats harbouring either a whole human faecal flora or single human strains of escherichia coli (em0) or bacteroides vulgatus (bv8h1) were fed for 7 weeks on semi-synthetic diets in which the protein source was either soya-bean meal (sm) or rape-seed meal (rm). for each bacterial status the rm-diet group was compared with the control group fed on the sm diet. the association of human faecal flora with the rm diet was responsible for reduced feed intake and reduced weight gain, ... | 1993 | 8399112 |
| identification of a circular intermediate in the transfer and transposition of tn4555, a mobilizable transposon from bacteroides spp. | transmissible cefoxitin (fx) resistance in bacteroides vulgatus cla341 was associated with the 12.5-kb, mobilizable transposon, tn4555, which encoded the beta-lactamase gene cfxa. transfer occurred by a conjugation-like mechanism, was stimulated by growth of donor cells with tetracycline (tc), and required the presence of a bacteroides chromosomal tcr element. transconjugants resistant to either fx, tc, or both drugs were obtained, but only fxr tcr isolates could act as donors of fxr in subseque ... | 1993 | 8386723 |
| analysis of intestinal flora of a patient with congenital absence of the portal vein. | a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ... | 1993 | 8364525 |
| application of indirect immunofluorescence to detection of dientamoeba fragilis trophozoites in fecal specimens. | an indirect fluorescent-antibody (ifa) assay was carried out to examine for the presence of dientamoeba fragilis trophozoites in preserved fecal specimens. antiserum to d. fragilis trophozoites was raised in a rabbit with a dixenic culture of d. fragilis (atcc 30948) from the american type culture collection. after absorption with klebsiella pneumoniae and bacteroides vulgatus, the immune rabbit serum was used for examination by the ifa assay. a total of 155 clinical samples were tested; 42 with ... | 1993 | 8349746 |
| human t cell adhesion to endothelium stimulated by membrane components extracted from strains of bacteroides vulgatus (member of b. fragilis group). | bacteroides fragilis is reported to be less virulent than other gram negative rods, existing in normal gut flora (e.g. enterobacteriaceae). lymphocytic infiltration, a typical finding in chronic infection, depends on cell extravasation and trafficking towards a site of antigen, a process that is regulated by endothelial expression of adhesion molecules. we have studied the ability of lipopolysaccharides and capsular polysaccharides of b. vulgatus group to induce human t cell adhesion to cultured ... | 1993 | 8239916 |
| antimicrobial resistance in human oral and intestinal anaerobic microfloras. | in the present study we determined the resistance patterns of anaerobic bacteria from human saliva and stool specimens and investigated whether there were significant differences in resistance between outpatients and hospitalized patients, regardless of whether they had received antimicrobial agents. no bacterial strains resistant to ampicillin, piperacillin, cefoxitin, cefuroxime, imipenem, clindamycin, doxycycline, chloramphenicol, or metronidazole were isolated from the saliva samples. howeve ... | 1993 | 8215280 |
| growth stimulator for bifidobacteria produced by propionibacterium freudenreichii and several intestinal bacteria. | a bifidogenic growth stimulator was present in the cell-free filtrate of propionibacterium freudenreichii 7025 culture and in the methanol extract fraction of the cells. several intestinal bacteria, such as bacteroides, enterobacter, and enterococcus, which also released a growth stimulator for bifidobacteria, may play an important role in regulation of a bifidobacterial population in colonic microflora. the water-soluble stimulator from the methanol extract of the cells was partially purified. ... | 1994 | 8182163 |
| delta 22-beta-muricholic acid in monoassociated rats and conventional rats. | bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ... | 1994 | 8177022 |
| sequence analysis of per-1 extended-spectrum beta-lactamase from pseudomonas aeruginosa and comparison with class a beta-lactamases. | we have determined the nucleotide sequence (embl accession number, z 21957) of the cloned chromosomal per-1 extended-spectrum beta-lactamase gene from a pseudomonas aeruginosa rnl-1 clinical isolate, blaper-1 corresponds to a 924-bp open reading frame which encodes a polypeptide of 308 amino acids. this open reading frame is preceded by a -10 and a -35 region consistent with a putative p. aeruginosa promoter. primer extension analysis of the per-1 mrna start revealed that this promoter was activ ... | 1994 | 8141562 |
| rapid detection of serpulina hyodysenteriae in diagnostic specimens by pcr. | a pcr assay for the detection of serpulina hyodysenteriae in diagnostic specimens was developed on the basis of sequence analysis of a recombinant clone designated pred3c6. clone pred3c6, which contained a 2.3-kb dna fragment unique to s. hyodysenteriae, was identified by screening a plasmid library of s. hyodysenteriae isolate b204 genomic dna in escherichia coli by colony immunoblot with the mouse monoclonal antibody 10g6/g10, which was produced against cell-free supernatant antigens from the ... | 1994 | 8077394 |
| susceptibility testing of dientamoeba fragilis atcc 30948 with iodoquinol, paromomycin, tetracycline, and metronidazole. | susceptibility testing was performed on dientamoeba fragilis atcc 30948 in a dixenic culture with klebsiella pneumoniae and bacteroides vulgatus. d. fragilis was cocultured with the bacteria in tygm-9 medium (atcc medium 1171). the activities of antiparasitic drugs were assessed by counting viable d. fragilis trophozoites with a hemacytometer by trypan blue exclusion. the minimal amebicidal concentrations of the following four drugs were determined: iodoquinol at 128 micrograms/ml, paromomycin a ... | 1994 | 8067755 |
| hematophagic histiocytosis: a clinicopathologic analysis of 23 cases with special reference to the association with peripheral t-cell lymphoma. | the clinicopathologic features of 23 patients with hematophagic histiocytosis (hh) are described. all of them exhibited increased histiocytes associated with hemophagocytosis in the marrow. the patients usually presented with fever, hepatosplenomegaly, lymphadenopathy, and cytopenia. the underlying illnesses were heterogeneous, including non-hodgkin's lymphoma in 17, systemic lupus erythematosus in one, diabetes mellitus in one, acute myelomonocytic leukemia in one, myelodysplastic syndrome in o ... | 1994 | 7920083 |
| origin of d-alanine present in urine of mutant mice lacking d-amino-acid oxidase activity. | urine of mutant ddy/dao- mice lacking d-amino-acid oxidase contained 13 times more d-alanine than that of normal ddy/dao+ mice. because d-alanine is a component of bacterial cell walls, the possibility that the urinary d-alanine came from intestinal bacteria was examined. in ddy/dao- mice that were made germ free at birth and reared in a germ-free environment, the quantity of urinary d-alanine was found to be at a low level comparable to that of the normal mice. when these germ-free mice were ma ... | 1993 | 7901999 |
| adherence properties of bacteroides vulgatus, the preponderant colonic organism of adult humans. | bacteroides vulgatus is the predominant intestinal bacterium of adult humans. the adhesins of this species were studied by using haemagglutination of rabbit erythrocytes and inhibition of this haemagglutination. eleven haemagglutinating strains were tested after incubation with chicken albumin, bovine serum albumin, human serum fibronectin, fibronectin fragment and n-acetyl neuraminic acid, and after they had been heated, treated with naio4, trypsin and d(+)-mannose. rabbit erythrocytes treated ... | 1994 | 7858350 |
| effect of h2-receptor antagonists on bile acid metabolism. | several reports have been presented concerning pronounced overgrowth of bacteria in gastric juices of patients treated with h2-receptor antagonists. however, there has been no report concerning influence of h2-receptor antagonists on jejunal flora. thus, to investigate the influence and its effect on bile acid metabolism, this study was performed: 1) to examine whether patients with gastric ulcers who have been treated with h2-receptor antagonists have positive bile acid breath tests due to bact ... | 1995 | 7735920 |
| immunocytochemical evidence of listeria, escherichia coli, and streptococcus antigens in crohn's disease. | infectious agents have long been suspected of playing a role in the initiation of crohn's disease. the objective of this study was to search for likely microbial agents in diseased tissues using immunocytochemical techniques. | 1995 | 7729631 |
| expression of a cloned cellulase/xylanase gene from prevotella ruminicola in bacteroides vulgatus, bacteroides uniformis and prevotella ruminicola. | a new shuttle vector, prh3 (8.7 kb), was constructed for use in prevotella/bacteroides host strains. this vector combines the prri2 replicon from p. ruminicola, pbluescript sequences and a tetq marker gene for selection in prevotella/bacteroides hosts. following insertion of a fragment carrying an endoglucanase/xylanase gene from p. ruminicola 23 into the multiple cloning site, the resulting construct, prh3x, was introduced into b. vulgatus 1447, b. uniformis 1100 and p. ruminicola 2202. this re ... | 1995 | 7592134 |
| analysis of bacteroides species by pyrolysis mass spectrometry. | two methods for the classification of isolates of the bacteroides fragilis group were compared in a study of 133 clinical isolates and 10 reference strains. there was broad similarity between the pattern of clusters in pyrolysis mass spectrometry (pyms), which reflects whole-cell composition, and that in conventional tests, which reflect nutritional interactions with the environment. a single large cluster was resolved by both approaches; this cluster comprised mainly strains identified as b. fr ... | 1995 | 7548532 |
| fibronectin and vitronectin binding of bacteroides fragilis and eight other species of the genus. | the fibronectin and vitronectin bindings of 152 strains belonging in 9 bacteroides species of different origins were tested by means of latex agglutination. 23% of the strains isolated from faeces exhibited fibronectin binding, as did 46% of the strains obtained from severe infections. most of the strains displaying fibronectin binding belonged to the species bacteroides fragilis or bacteroides vulgatus. the binding could be inhibited by preincubation of the cells with an excess amount of fibron ... | 1994 | 7532044 |
| the use of pcr to monitor the population abundance of six human intestinal bacterial species in an in vitro semicontinuous culture system. | six pcr primer sets complementary to the 16s rdnas (rrna genes) were developed and shown to be specific for the following anaerobic bacteria: clostridium clostridiiforme, c. perfringens, c. leptum, bacteroides vulgatus, b. distasonis, and b. thetaiotaomicron, respectively. these primers were used for pcr to detect and monitor the bacteria in a semicontinuous culture system designed to mimic intestinal microflora in the human gastrointestinal tract. except for c. perfringens, the five species of ... | 1994 | 7529205 |
| the toxicity of antigens extracted from strains of bacteroides vulgatus from different origins to chicken embryos. | the toxicity of purified lipopolysaccharides (lps) and capsular polysaccharides (cps) extracted from 3 strains of bacteroides vulgatus to 11 day old chicken embryos was examined. the three b. vulgatus strains were isolated from different sources. the celd50 (chicken embryos lethal dose 50%) of these preparations was determined. all antigens were shown to be lethal to chick embryos. the toxicity of lps of the strains isolated from clinical sources was higher than that of the capsular antigens. th ... | 1994 | 7526620 |
| the cell-surface antigens of bacteroides vulgatus. | the cell surface of bacteroides vulgatus was examined by electron microscopy. the outer membrane complex was removed by edta and mild sonication and the antigens of this complex were characterized by enzyme-linked immunosorbent assay and crossed immunoelectrophoresis. the species-specific antigen was identified and was shown to be the major outer membrane protein with a molecular weight of 100 000. | 1981 | 7334331 |
| production of experimental ulcerative colitis in gnotobiotic guinea pigs with simplified microflora. | conventional guinea pigs provided with a solution of 5% (wt/vol) degraded carrageenan as the sole source of oral fluids developed ulcerations of their ceca and large intestines within 30 days. similar lesions were not detected in germfree guinea pigs treated in an identical manner, suggesting that an intestinal microflora was necessary for development of intestinal lesions. to simplify the bacterial flora required for production of cecal ulcerations, 10 pools consisting of 10 bacterial strains e ... | 1981 | 7216487 |
| production of lesions in gnotobiotic mice by inoculation with treponema hyodysenteriae. | treponema hyodysenteriae was established in the ceca of gnotobiotic mice in the absence of other organisms. superficial mucosal lesions characteristic of swine dysentery were present in the ceca of mice inoculated with t. hyodysenteriae in combination with viable bacteroides vulgatus. deep crypt necrosis was detected in the ceca of mice inoculated with t. hyodysenteriae alone. | 1981 | 7216455 |
| the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract. | the development of the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract was studied in 31 infants during the first 10 days of life. specimens were collected from the umbilicus, mouth and gastro-intestinal tract on the pre-operative day, at operation and on post-operative days 1, 2, 3, 5, 7 and 10. bacteria were isolated semi-quantitatively on a variety of plain and selective media and identified by conventional methods.staphylococcus albus was the predomin ... | 1982 | 7057028 |
| effect of secreted bacteroides proteases on human intestinal brush border hydrolases. | selected bacteroides species secreted various amounts of protease and glycosidase into their growth medium. bacteroides vulgatus, distasonis, and ovatus secreted the most (31-60% of total). the secreted protease was similar in action to the protease within the organism, in that it had a broad ph optimum of 6-9, a k(m app.) for casein of 0.1 mum, and was inhibited by benzamidine, phenylmethylsulfonyl fluoride, diisopropylfluorophosphate (difp), and by an elastase inhibitor, ac(ala)(3)alach(2)cl. ... | 1980 | 6995483 |
| specific strains of bacteroides species in human fecal flora as measured by deoxyribonucleic acid homology. | membrane competition homology experiments were used to compare bacteroides uniformis and bacteroides vulgatus isolates obtained from fecal samples from different individuals and isolates obtained from fecal samples of single individuals. isolates of b. uniformis, when isolated from different individuals, had interstrain deoxyribonucleic acid homology values that ranged from 63 to 95%, with most of the values being in the 70 to 85% range. when isolates obtained from a single individual were compa ... | 1980 | 6990866 |
| properties of a new penicillinase type produced by bacteroides fragilis. | a highly penicillin-resistant strain of bacteroides fragilis, strain gn11499, was found among 80 clinical isolates of the b.fragilis group and appears to produce a new type of penicillinase. penicillinase activity was detected in crude extracts and had a specific activity of 0.25 u/mg of protein. about 20% of the enzyme was released into the surrounding medium during growth. the enzyme hydrolyzed ampicillin and cloxacillin more rapidly than it did penicillin g, carbenicillin, and cephaloridine. ... | 1982 | 6983857 |