Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| visualizing evolutionary relationships of multidomain proteins: an example from receiver (rec) domains of sensor histidine kinases in the candidatus maribeggiatoa str. orange guaymas draft genome. | for multidomain proteins, evolutionary changes may occur at the domain as well as the whole-protein level. an example is presented here, with suggestions for how such complicated relationships might be visualized. earlier analysis of the candidatus maribeggiatoa str. orange guaymas (boguay; gammaproteobacteria) single-filament draft genome found evidence of gene exchange with the phylogenetically distant cyanobacteria, particularly for sensory and signal transduction proteins. because these are ... | 2016 | 27895624 |
| biogeochemical and hydrological drivers of the dynamics of vibrio species in two patagonian estuaries. | the ecology of the most relevant vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in argentinian patagonia. vibrio cholerae and vibrio parahaemolyticus were reported in >29% of cases at the río colorado and río negro estuaries. neither the pandemic serogroups of vibrio cholerae o1, vibrio cholerae o139 nor the cholera toxin gene were detected in this study. however, several strains of v. cholerae (not o1 or o139) are able to ca ... | 2017 | 27871750 |
| capsular polysaccharide production and serum survival of vibrio vulnificus are dependent on antitermination control by rfah. | the human pathogen vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (cps) and a translucent phenotype that produces little or no cps and is attenuated. here, we found that a v. vulnificus mutant defective for rfah antitermination control showed a diminished capacity to undergo phase variation and displayed significantly reduced distal gene expression within the group i cps operon. moreover, the rfah mu ... | 2016 | 27859050 |
| pathogenic mechanisms of actin cross-linking toxins: peeling away the layers. | actin cross-linking toxins are produced by gram-negative bacteria from vibrio and aeromonas genera. the toxins were named actin cross-linking domains (acd), since the first and most of the subsequently discovered acds were found as effector domains in larger martx and vgrg toxins. among recognized human pathogens, acd is produced by vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. upon delivery to the cytoplasm of a host cell, acd covalently cross-links actin monomers into non-polym ... | 2017 | 27858184 |
| non-cholera vibrios: the microbial barometer of climate change. | there is a growing interest in the role of climate change in driving the spread of waterborne infectious diseases, such as those caused by bacterial pathogens. one particular group of pathogenic bacteria - vibrios - are a globally important cause of diseases in humans and aquatic animals. these gram-negative bacteria, including the species vibrio vulnificus, vibrio parahaemolyticus and vibrio cholerae, grow in warm, low-salinity waters, and their abundance in the natural environment mirrors ambi ... | 2017 | 27843109 |
| a tfpi-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, cynoglossus semilaevis. | tissue factor pathway inhibitor 1 (tfpi-1) is a serine protease inhibitor that inhibits tissue factor (tf)-mediated coagulation. the c-terminal region of tfpi-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. in a previous study, a c-terminal peptide, tc24 (with 24 amino acids), derived from tongue sole (cynoglossus semilaevis) tfpi-1, was synthesized and found antibacterial against micrococcus luteus. in the present study, the antibacterial spectru ... | 2017 | 27840169 |
| season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea. | vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ... | 2017 | 27836844 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus in the clam ruditapes philippinarum (adams & reeve, 1850) from emilia romagna and sardinia, italy. | marine vibrios, vibrio parahaemolyticus, v. vulnificus and v. cholerae are responsible of the majority of food-borne human infections by consumption of bivalve shellfish. the aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the manila clam r. philippinarum from emilia romagna (er) and sardinia (sr) regions, italy. isolation was performed on chromagar(tm) vibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) agar and m- ... | 2016 | 27800436 |
| vibrio vulnificus type 6 secretion system 1 contains anti-bacterial properties. | vibrio vulnificus is a bacterium responsible for severe gastroenteritis, sepsis and wound infections. gastroenteritis and sepsis are commonly associated with the consumption of raw oysters, whereas wound infection is often associated with the handling of contaminated fish. although classical virulence factors of this emerging pathogen are well characterised, there remains a paucity of knowledge regarding the general biology of this species. to investigate the presence of previously unreported vi ... | 2016 | 27798649 |
| differences in abundances of total vibrio spp., v. vulnificus, and v. parahaemolyticus in clams and oysters in north carolina. | filter feeding shellfish can concentrate pathogenic bacteria, including vibrio vulnificus and vibrio parahaemolyticus, as much as 100-fold from the overlying water. these shellfish, especially clams and oysters, are often consumed raw, providing a route of entry for concentrated doses of pathogenic bacteria into the human body. the numbers of foodborne infections with these microbes are increasing, and a better understanding of the conditions that might trigger elevated concentrations of these b ... | 2017 | 27793822 |
| two new benzoate derivatives and one new phenylacetate derivative from a marine-derived fungus engyodontium album. | two new benzoate derivatives, ethyl 3,5-dimethoxy-2-propionylbenzoate (1) and ethyl 3,5-dihydroxy-2-propionylbenzoate (2), and one new phenylacetate derivative, ethyl 3,5-dimethoxy-2-propionylphenylacetate (3), together with 9 known compounds, were isolated from the fermentation of engyodontium album derived from deep sea sediment. their structures were elucidated by spectroscopic techniques, such as nmr, ir and hresims. compound 3 exhibited inhibitory activities against methicillin-resistant st ... | 2017 | 27750436 |
| a silkworm infection model to investigate vibrio vulnificus virulence genes. | the halophilic marine bacterium, vibrio vulnificus, occasionally causes fatal septicemia in immunocompromised patients. mice are commonly used as experimental animals to investigate the virulence of v. vulnificus, however, a large number of mice are generally required for bioassays. the present study examined whether the invertebrate species, silkworms, can be used instead of mice to investigate v. vulnificus virulence. when the silkworms were inoculated with 1.2x107 colony forming units of v. v ... | 2016 | 27748924 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| quantification of vibrio species in oysters from the gulf of mexico with two procedures based on mpn and pcr. | oysters can accumulate potentially pathogenic water bacteria. the objective of this study was to compare two procedures to quantify vibrio species present in oysters to determine the most sensitive method. we analyzed oyster samples from the gulf of mexico, commercialized in mexico city. the samples were inoculated in tubes with alkaline peptone water (apw), based on three tubes and four dilutions (10(-1) to 10(-4)). from these tubes, the first quantification of vibrio species was performed (mos ... | 2016 | 27709461 |
| o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum. | the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ... | 2017 | 27624095 |
| rapidly developing and fatal vibrio vulnificus wound infection. | 2016 | 27617208 | |
| rt-pcr-dgge analysis to elucidate the dominant bacterial species of industrial spanish-style green table olive fermentations. | this paper describes the dominant bacterial species metabolically active through the industrial production of spanish-style manzanilla and gordal olives. for this purpose, samples (brines and fruits) obtained at 0, 15, and 90 fermentation days were analyzed by a culture-independent approach to determine viable cells by reverse transcription of rna and further pcr-dgge analysis, detecting at least 7 different species. vibrio vulnificus, lactobacillus plantarum group, and lactobacillus parafarragi ... | 2016 | 27582739 |
| a c-type lectin that inhibits bacterial infection and facilitates viral invasion in black rockfish, sebastes schlegelii. | c-type lectins (ctls) are important pattern recognition receptors (prrs) that play vital roles in innate immunity. in teleosts, a number of ctls have been reported, but their in vivo effects on host defense are still limited. in this study, a ctl homolog (sslec1) was identified from black rockfish, sebastes schlegelii, and its structure, expression and biological function was analyzed. the open reading frame of sslec1 is 633 bp, with a 5'- untranslated region (utr) of 36 bp and a 3'- utr of 117 ... | 2016 | 27569982 |
| faraday cage-type electrochemiluminescence immunosensor for ultrasensitive detection of vibrio vulnificus based on multi-functionalized graphene oxide. | a novel faraday cage-type electrochemiluminescence (ecl) immunosensor devoted to the detection of vibrio vulnificus (vv) was fabricated. the sensing strategy was presented by a unique faraday cage-type immunocomplex based on immunomagnetic beads (imbs) and multi-functionalized graphene oxide (go) labeled with (2,2'-bipyridine)(5-aminophenanthroline)ruthenium (ru-nh2). the multi-functionalized go could sit on the electrode surface directly due to the large surface area, abundant functional groups ... | 2016 | 27565793 |
| a rare glimpse into the morbid world of necrotising fasciitis: flesh-eating bacteria vibrio vulnificus. | necrotising fasciitis is one of the fatal skin and soft tissue infections. vibrio vulnificus is a rare cause of necrotising fasciitis; however, the disease is one of the major manifestations of the bacteria. here, we report one such case in a middle-aged male patient. he presented with the signs of bilateral lower limb cellulitis and altered sensorium. v. vulnificus was isolated from blood culture and also from debrided tissue. though the organism is well characterised, it is a rare causative ag ... | 2017 | 27514968 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| role of extracellular matrix protein caba in resistance of vibrio vulnificus biofilms to decontamination strategies. | biofilms are recalcitrant and raise safety problems in the food industry. in this study, the role of caba, an extracellular matrix protein, in the resistance of the biofilms of vibrio vulnificus, a foodborne pathogen, to decontamination strategies was investigated. biofilms of the caba mutant revealed reduced resistance to detachment by vibration and disinfection by sodium hypochlorite compared to the biofilms of the parental wild type in vitro. the reduced resistance of the caba mutant biofilms ... | 2016 | 27485973 |
| characterization of clinical and environmental isolates of vibrio cidicii sp. nov., a close relative of vibrio navarrensis. | four vibrio spp. isolates from the historical culture collection at the centers for disease control and prevention, obtained from human blood specimens (n=3) and river water (n=1), show characteristics distinct from those of isolates of the most closely related species, vibrio navarrensis and vibrio vulnificus, based on phenotypic and genotypic tests. they are specifically adapted to survival in both freshwater and seawater, being able to grow in rich media without added salts as well as salinit ... | 2016 | 27468862 |
| development of a quadruplex loop-mediated isothermal amplification assay for field detection of four vibrio species associated with fish disease. | a quadruplex loop-mediated isothermal amplification (lamp) method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. four sets of species-specific primers were designed with different restriction sites contained in the inner primers. the quadruplex lamp method could distinguish four vibrio species via the subsequent restriction enzyme analysis. the sensitivity of the quadruplex lamp meth ... | 2016 | 27468405 |
| vibrio parahaemolyticus and vibrio vulnificus in south america: water, seafood and human infections. | the bacterial species, vibrio parahaemolyticus and vibrio vulnificus, are ubiquitous in estuaries and coastal waters throughout the world, but they also happen to be important human pathogens. they are concentrated by filter-feeding shellfish which are often consumed raw or undercooked, providing an important potential route of entry for an infective dose of these bacteria. vibrio parahaemolyticus can cause abdominal cramping, nausea, diarrhoea, vomiting, chills and fever. vibrio vulnificus can ... | 2016 | 27459915 |
| in vitro and in vivo anti-vibrio vulnificus activity of psammaplin a, a natural marine compound. | vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. in the present study, the antimicrobial activity of natural marine product-derived compounds against v. vulnificus, were investigated in vitro and in vivo. twelve pure compounds were isolated from natural marine products and their inhibitory effects on v. vulnificus-induced cytotoxicity were determined in int‑407 cells. among the 12 pure compounds tested, treatment with psammaplin a significantly s ... | 2016 | 27431807 |
| induced disruption of the iron-regulatory hormone hepcidin inhibits acute inflammatory hypoferraemia. | withdrawal of iron from serum (hypoferraemia) is a conserved innate immune antimicrobial strategy that can withhold this critical nutrient from invading pathogens, impairing their growth. hepcidin (hamp1) is the master regulator of iron and its expression is induced by inflammation. mice lacking hamp1 from birth rapidly accumulate iron and are susceptible to infection by blood-dwelling siderophilic bacteria such as vibrio vulnificus. in order to study the innate immune role of hepcidin against a ... | 2016 | 27423740 |
| iron and fur in the life cycle of the zoonotic pathogen vibrio vulnificus. | in this study, we aimed to analyze the global response to iron in the broad-range host pathogen vibrio vulnificus under the hypothesis that iron is one of the main signals triggering survival mechanisms both inside and outside its hosts. to this end, we selected a strain from the main zoonotic clonal-complex, obtained a mutant in the ferric-uptake-regulator (fur), and analyzed their transcriptomic profiles in both iron-excess and iron-poor conditions by using a strain-specific microarray platfor ... | 2016 | 27348505 |
| complete genome sequence of vibrio vulnificus forc_017 isolated from a patient with a hemorrhagic rash after consuming raw dotted gizzard shad. | vibrio vulnificus, a resident in the human gut, is frequently found in seafood, causing food-borne illnesses including gastroenteritis and severe septicemia. while v. vulnificus has been known to be one of the major food-borne pathogens, pathogenicity and virulence factors are not fully understood yet. to extend our understanding of the pathogenesis of v. vulnificus at the genomic level, the genome of v. vulnificus forc_017 isolated from a female patient experiencing a hemorrhagic rash was compl ... | 2016 | 27325916 |
| deacylated lipopolysaccharides inhibit biofilm formation by gram-negative bacteria. | the extracellular polysaccharides of vibrio vulnificus play different roles during biofilm development. among them, the effect of lipopolysaccharide (lps), which is crucial for bacterial adherence to surfaces during the initial stage of biofilm formation, on the formation process was examined using various types of lps extracts. exogenously added lps strongly inhibited biofilm formation in a dose-dependent manner. in addition, the exogenous addition of a deacylated form of lps (dlps) also inhibi ... | 2016 | 27294580 |
| [postoperative necrotizing fasciitis: a rare and fatal complication]. | postoperative parietal complications can be exceptionally severe and serious threatening vital prognosis. necrotizing fasciitis is a rare infection of the skin and deep subcutaneous tissues, spreading along fascia and adipose tissue. it is mainly caused by group a streptococcus (streptococcus pyogenes) but also by other bacteria such as vibrio vulnificus, clostridium perfringens or bacteroides fragilis. necrotizing fasciitis is a real surgical and medical emergency. we report, in this study, a v ... | 2016 | 27279950 |
| oxyr2 functions as a three-state redox switch to tightly regulate production of prx2, a peroxiredoxin of vibrio vulnificus. | the bacterial transcriptional regulator oxyr is known to function as a two-state redox switch. oxyr senses cellular levels of h2o2 via a "sensing cysteine" that switches from the reduced to a disulfide state upon h2o2 exposure, inducing the expression of antioxidant genes. the reduced and disulfide states of oxyr, respectively, bind to extended and compact regions of dna, where the reduced state blocks and the oxidized state allows transcription and further induces target gene expression by inte ... | 2016 | 27268058 |
| in vitro and in vivo antibacterial activity of tigecycline against vibrio vulnificus. | the aim of this study is to investigate the role of tigecycline in vibrio vulnificus infection. | 2016 | 27260781 |
| vibrio vulnificus vvha induces autophagy-related cell death through the lipid raft-dependent c-src/nox signaling pathway. | vvha, a virulent factor of vibrio (v.) vulnificus, induces acute cell death in a destructive manner. autophagy plays an important role in cell death, but the functional role of vvha in autophagy-related cell death has not been elucidated yet. we found that rvvha significantly increased lc3 puncta formation and autophagic flux in promoting the cell death of human intestinal epithelial caco-2 cells. the cell death induced by rvvha was independent of lysosomal permeabilizaton and caspase activation ... | 2016 | 27250250 |
| prevalence and distribution of vibrio spp. in wild aquatic birds of the southern caribbean sea, venezuela, 2011-12. | vibrio spp. are associated with waterbirds mainly in temperate latitudes. we evaluated the prevalence and distribution of vibrio spp. from fecal samples of resident and migratory aquatic birds collected during october 2011 and march 2012 at two coastal sites in the tropical southern caribbean sea. we amplified dna by pcr in 40% of samples, resulting in 47% and 36% estimated prevalence for resident and migratory birds in cuare wildlife refuge, and 33% and 44% in margarita island, respectively. we ... | 2016 | 27224211 |
| glucose induces delocalization of a flagellar biosynthesis protein from the flagellated pole. | to survive in a continuously changing environment, bacteria sense concentration gradients of attractants or repellents, and purposefully migrate until a more favourable habitat is encountered. while glucose is known as the most effective attractant, the flagellar biosynthesis and hence chemotactic motility has been known to be repressed by glucose in some bacteria. to date, the only known regulatory mechanism of the repression of flagellar synthesis by glucose is via downregulation of the camp l ... | 2016 | 27218601 |
| effects of intertidal harvest practices on levels of vibrio parahaemolyticus and vibrio vulnificus bacteria in oysters. | vibrio parahaemolyticus and vibrio vulnificus can grow rapidly in shellfish subjected to ambient air conditions, such as during intertidal exposure. in this study, levels of total and pathogenic (tdh(+) and/or trh(+)) v. parahaemolyticus and total v. vulnificus were determined in oysters collected from two study locations where intertidal harvest practices are common. samples were collected directly off intertidal flats, after exposure (ambient air [washington state] or refrigerated [new jersey] ... | 2016 | 27208133 |
| norovirus (nov) specific protective immune responses induced by recombinant p dimer vaccine are enhanced by the mucosal adjuvant flab. | noroviruses (novs) are a major cause of childhood gastroenteritis and foodborne diseases worldwide. lack of appropriate animal models or cell-based culture systems makes the development and evaluation of nov-specific vaccines a daunting task. vp1 is the major capsid protein of the novs that acts as a binding motif to human histo-blood group antigens (hbgas) through its protruding 2 (p2) domain and can serve as a protective antigen candidate for vaccine development. | 2016 | 27184355 |
| iron- and quorum-sensing signals converge on small quorum-regulatory rnas for coordinated regulation of virulence factors in vibrio vulnificus. | vibrio vulnificus is a marine bacterium that causes human infections resulting in high mortality. this pathogen harbors five quorum-regulatory rnas (qrr1-5) that affect the expression of pathogenicity genes by modulating the expression of the master regulator smcr. the qrr genes are activated by phosphorylated luxo to different degrees; qrr2 is strongly activated; qrr3 and qrr5 are moderately activated, and qrr1 and qrr4 are marginally activated and are the only two that do not respond to cell d ... | 2016 | 27151217 |
| vibrio vulnificus infection and liver cirrhosis: a potentially lethal combination. | we present a case of a 40-year-old man with decompensated alcoholic liver cirrhosis presenting with atraumatic cellulitis of one extremity and severe sepsis that rapidly progressed to compartment syndrome despite broad-spectrum antibiotics. local cultures following debridement revealed vibrio vulnificus, and subsequent history revealed consumption of raw oysters 48 h before presentation. our case points out the unique susceptibility of those with cirrhosis and elevated iron saturation to vibrio ... | 2016 | 27151052 |
| spatiotemporal dynamics of vibrio spp. within the sydney harbour estuary. | vibrio are a genus of marine bacteria that have substantial environmental and human health importance, and there is evidence that their impact may be increasing as a consequence of changing environmental conditions. we investigated the abundance and composition of the vibrio community within the sydney harbour estuary, one of the most densely populated coastal areas in australia, and a region currently experiencing rapidly changing environmental conditions. using quantitative pcr (qpcr) and vibr ... | 2016 | 27148171 |
| biotreatment of petrochemical wastewater: a case study from northern tunisia. | a full-scale study has been conducted to assess the bioaugmentation efficiency of trickling filter process to treat petrochemical wastewater from a lubricant industry recycling waste oils. during 45 weeks, the organic loading rate (olr) in the trickling filter was increased stepwise from 0.9 to 4 kg of chemical oxygen demand (cod)/(m3·day) at the end of the upgrading period as the flow rate (fr) reached the value of 30 m3/day. the removal, obtained in terms of percentage, for cod ranged from 60 ... | 2017 | 27094680 |
| successful treatment of a case of necrotizing fasciitis due to vibrio vulnificus in a cold climate in japan. | vibrio vulnificus infection often occurs in warm regions, frequently leading to necrotizing fasciitis, sepsis, and death. we herein report a rare case presenting in a cold climate region in northern japan, aomori district, of a v. vulnificus infection complicated by necrotizing fasciitis and septic shock. the patient's prior history of injury and typical clinical course were helpful clues to the diagnosis of v. vulnificus infection, and early initiation of antimicrobial treatment saved his life. ... | 2016 | 27086822 |
| antibiotic resistance of vibrio parahaemolyticus and vibrio vulnificus in various countries: a review. | vibrio parahaemolyticus and vibrio vulnificus are the leading causes of seafood associated infections and mortality in the united states. the main syndromes caused by these pathogens are gastroenteritis, wound infections, and septicemia. this article reviewed the antibiotic resistance profile of v. parahaemolyticus and v. vulnificus in the united states and other countries including italy, brazil, philippines, malaysia, thailand, china, india, iran, south africa and australia. the awareness of a ... | 2016 | 27052711 |
| phaeobacter inhibens as biocontrol agent against vibrio vulnificus in oyster models. | molluscan shellfish can cause food borne diseases and here we investigated if addition of vibrio-antagonising bacteria could reduce vibrio vulnificus in model oyster systems and prevent its establishment in live animals. phaeobacter inhibens, which produces an antibacterial compound, tropodithietic acid (tda), inhibited v. vulnificus as did pure tda (mic of 1-3.9 μm). p. inhibens dsm 17395 (at 10(6) cfu/ml) eradicated 10(5) cfu/ml v. vulnificus cmcp6 (a rifampicin resistant variant) from a co-cu ... | 2016 | 27052703 |
| [when should notification of an infectious disease be mandatory? a decision aid to determine whether mandatory notification is justified]. | in the netherlands, all physicians are required to report cases of certain infectious diseases to the public health services, to allow appropriate control measures. in recent years, various requests have been submitted to add certain infectious diseases to the list of notifiable diseases. in order to decide whether such a request should be granted, we developed a structured decision aid based on a range of existing criteria for mandatory notification, applied in the netherlands and other countri ... | 2016 | 27050495 |
| temperature change induces the expression of vuua encoding vulnibactin receptor and crp encoding cyclic amp receptor protein in vibrio vulnificus. | upon entering the human body, vibrio vulnificus, a gram-negative marine bacterium, must withstand a temperature change (tc) from 25 to 37 °c. this bacterium acquires iron mainly via the vulnibactin receptor (vuua)-mediated iron uptake system (ius), which is under the positive control of cyclic amp receptor protein (crp), a global regulator responsible for catabolite repression. in this study, we examined the effect of tc on the expression of vuua and crp, and the reciprocal relation between vuua ... | 2016 | 27016238 |
| isolation and molecular identification of vibrio spp. by sequencing of 16s rdna from seafood, meat and meat products in libya. | the genus vibrio includes several food-borne pathogens that cause a spectrum of clinical conditions including septicemia, cholera and milder forms of gastroenteritis. several vibrio spp. are commonly associated with food-borne transmission including vibrio cholerae, vibrio parahemolyticus, and vibrio vulnificus. microbiological analysis for enumeration and isolation of vibrio spp. were carried out for a total of 93 samples of seafood, meat and meat products from different geographic localities i ... | 2016 | 27004169 |
| detection and quantification of vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus in coastal waters of guinea-bissau (west africa). | v. cholerae, v. parahaemolyticus, and v. vulnificus are recognized human pathogens. although several studies are available worldwide, both on environmental and clinical contexts, little is known about the ecology of these vibrios in african coastal waters. in this study, their co-occurrence and relationships to key environmental constraints in the coastal waters of guinea-bissau were examined using the most probable number-polymerase chain reaction (mpn-pcr) approach. all vibrio species were uni ... | 2016 | 26940502 |
| functional analysis of vibrio vulnificus orthologs of escherichia coli rraa and rnase e. | rnase e plays an important role in the degradation and processing of rna in escherichia coli. the enzymatic activity of rnase e is controlled by the protein inhibitors rraa and rrab. the marine pathogenic bacterium vibrio vulnificus also contains homologs of rnase e and rraa, designated as rnase ev, rraav1, and rraav2. here, we report that rraav1 actively inhibits the enzymatic activity of rnase ev in vivo and in vitro by interacting with the c-terminal domain of rnase ev. coexpression of rraav1 ... | 2016 | 26888524 |
| vibrio bacteria in raw oysters: managing risks to human health. | the human-pathogenic marine bacteria vibrio vulnificus and v. parahaemolyticus are strongly correlated with water temperature, with concentrations increasing as waters warm seasonally. both of these bacteria can be concentrated in filter-feeding shellfish, especially oysters. because oysters are often consumed raw, this exposes people to large doses of potentially harmful bacteria. various models are used to predict the abundance of these bacteria in oysters, which guide shellfish harvest policy ... | 2016 | 26880841 |
| enterobacteria and vibrio from macrobrachium amazonicum prawn farming in fortaleza, ceará, brazil. | to investigate the isolation of enterobacteria associated with macrobrachium amazonicum (m. amazonicum) farming and evaluate the in vitro antimicrobial susceptibility of vibrio strains. | 2016 | 26851782 |
| distribution of fatal vibrio vulnificus necrotizing skin and soft-tissue infections: a systematic review and meta-analysis. | vibrio vulnificus necrotizing skin and soft tissue infections (vnsstis), which have increased significantly over the past few decades, are still highly lethal and disabling diseases despite advancing antibiotic and infection control practices. we, therefore, examined the spatiotemporal distribution of worldwide reported episodes and associated mortality rates of vnsstis between 1966 and 2014. the pubmed and cochrane library databases were systematically searched for observational studies on pati ... | 2016 | 26844475 |
| electrotransfer of the tilapia piscidin 3 and tilapia piscidin 4 genes into skeletal muscle enhances the antibacterial and immunomodulatory functions of oreochromis niloticus. | tilapia piscidin 3 (tp3) and tilapia piscidin 4 (tp4) are antimicrobial peptides recently isolated from oreochromis niloticus. we previously showed that synthetic tp3 and tp4 possessed antimicrobial activities. here, we analyzed the bactericidal abilities and immunomodulatory properties of these amps following the electroporation of pcmv-gfp-tp3 or pcmv-gfp-tp4 plasmid into tilapia (o. niloticus) muscle and subsequent infection with vibrio vulnificus or streptococcus agalactiae. prior overexpres ... | 2016 | 26828260 |
| rapid and sensitive detection of vibrio parahaemolyticus and vibrio vulnificus by multiple endonuclease restriction real-time loop-mediated isothermal amplification technique. | vibrio parahaemolyticus and vibrio vulnificus are two marine seafood-borne pathogens causing severe illnesses in humans and aquatic animals. in this study, a recently developed novel multiple endonuclease restriction real-time loop-mediated isothermal amplification technology (mert-lamp) were successfully developed and evaluated for simultaneous detection of v. parahaemolyticus and v. vulnificus strains in only a single reaction. two mert-lamp primer sets were designed to specifically target tox ... | 2016 | 26797596 |
| structural insights on identification of potential lead compounds targeting wbpp in vibrio vulnificus through structure-based approaches. | wbpp encoding udp-glcnac c4 epimerase is responsible for the activation of virulence factor in marine pathogen vibrio vulnificus (v. vulnificus) and it is linked to many aquatic diseases, thus making it a potential therapeutic target. there are few reported compounds that include several natural products and synthetic compounds targeting vibrio sp, but specific inhibitor targeting wbpp are unavailable. here, we performed structure-based virtual screening using chemical libraries such as binding, ... | 2016 | 26795501 |
| prevalence, characteristics and ecology of vibrio vulnificus found in new zealand shellfish. | in new zealand, there have been no known cases of foodborne diseases linked to vibrio vulnificus and shellfish consumption, but two cases of wound infection have been reported. we evaluated the distribution, the effect of environmental parameters, the pheno-genotypic profile and the growth characteristics of strains isolated from shellfish. | 2016 | 26788798 |
| evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products. | antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ... | 2010 | 26781242 |
| martx effector cross kingdom activation by golgi-associated adp-ribosylation factors. | vibrio vulnificus infects humans and causes lethal septicemia. the primary virulence factor is a multifunctional-autoprocessing repeats-in-toxin (martx) toxin consisting of conserved repeats-containing regions and various effector domains. recent genomic analyses for the newly emerged v. vulnificus biotype 3 strain revealed that its martx toxin has two previously unknown effector domains. herein, we characterized one of these domains, domain x (dmxvv ). a structure-based homology search revealed ... | 2016 | 26780191 |
| occurrence and molecular characterisation of vibrio parahaemolyticus in crustaceans commercialised in venice area, italy. | infections due to the pathogenic human vibrios, vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus, are mainly associated with consumption of raw or partially cooked bivalve molluscs. at present, little is known about the presence of vibrio species in crustaceans and the risk of vibriosis associated with the consumption of these products. the aim of the present study was to evaluate the prevalence and concentration of the main pathogenic vibrio spp. in samples of crustaceans (n=143) ... | 2016 | 26773255 |
| regulatory characteristics of vibrio vulnificus gbpa gene encoding a mucin-binding protein essential for pathogenesis. | binding to mucin is the initial step for enteropathogens to establish pathogenesis. an open reading frame, gbpa, of vibrio vulnificus was identified and characterized in this study. compared with wild type, the gbpa mutant was impaired in binding to mucin-agar and the mucin-secreting ht29-methotrexate cells, and the impaired mucin binding was restored by the purified gbpa provided exogenously. the gbpa mutant had attenuated virulence and ability of intestinal colonization in a mouse model, indic ... | 2016 | 26755724 |
| edwardsiella tarda bacteremia. a rare but fatal water- and foodborne infection: review of the literature and clinical cases from a single centre. | edwardsiella tarda bacteremia (etb) can be a fatal disease in humans. | 2017 | 26744588 |
| role of the histone-like nucleoid structuring protein (h-ns) in the regulation of virulence factor expression and stress response in vibrio vulnificus. | temperature is one of the important parameters regulating the expression of virulence factors in bacteria. the global regulator, a histone-like nucleoid structuring protein (h-ns), is known to play a crucial role in this regulation. in the present study, we first clarified the role of h-ns in the temperature-dependent regulation of virulence factor production in vibrio vulnificus, including that of the cytolytic toxin (v. vulnificus hemolysin: vvh) and the proteolytic enzyme (v. vulnificus prote ... | 2015 | 26699858 |
| virulence profiles of vibrio vulnificus in german coastal waters, a comparison of north sea and baltic sea isolates. | vibrio vulnificus is a halophilic bacterium of coastal environments known for sporadically causing severe foodborne or wound infections. global warming is expected to lead to a rising occurrence of v. vulnificus and an increasing incidence of human infections in northern europe. so far, infections in germany were exclusively documented for the baltic sea coast, while no cases from the north sea region have been reported. regional variations in the prevalence of infections may be influenced by di ... | 2015 | 26694432 |
| vibrio spp. from macrobrachium amazonicum prawn farming are inhibited by moringa oleifera extracts. | to investigate the in vitro antimicrobial potential of extracts of stem, leaves, flowers, pods and seeds of moringa oleifera (m. oleifera) against vibrio spp. from hatchery water and the prawn macrobrachium amazonicum. | 2015 | 26614991 |
| septic arthritis and subsequent fatal septic shock caused by vibrio vulnificus infection. | vibrio vulnificus is a rare but potential fatal bacterium that can cause severe infections. wound infections, primary sepsis and gastroenteritis are the most common clinical features. septic arthritis caused by v. vulnificus is an atypical presentation that has been reported in only two case reports; however, it has not been previously noted in denmark. the authors report a case of septic arthritis caused by v. vulnificus in an immunocompromised patient. the disease progressed to severe sepsis a ... | 2015 | 26604231 |
| genotypic diversity and population structure of vibrio vulnificus strains isolated in taiwan and korea as determined by multilocus sequence typing. | the genetic diversity and population structure of vibrio vulnificus isolates from korea and taiwan were investigated using pcr-based assays targeting putative virulence-related genes and multilocus sequence typing (mlst). box-pcr genomic fingerprinting identified 52 unique genotypes in 84 environmental and clinical v. vulnificus isolates. the majority (> 50%) of strains had pathogenic genotypes for all loci tested; moreover, many environmental strains had pathogenic genotypes. although significa ... | 2015 | 26599487 |
| purification and host specificity of predatory halobacteriovorax isolates from seawater. | halobacteriovorax (formerly bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. four strains of halobacteriovorax originally isolated in vibrio parahaemolyticus o3:k6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. this technique was essential, since 0.45-μm filtration alone was unable to remove infectious vibrio minicells, as determined by ... | 2016 | 26590288 |
| survey on antimicrobial resistance patterns in vibrio vulnificus and vibrio cholerae non-o1/non-o139 in germany reveals carbapenemase-producing vibrio cholerae in coastal waters. | an increase in the occurrence of potentially pathogenic vibrio species is expected for waters in northern europe as a consequence of global warming. in this context, a higher incidence of vibrio infections is predicted for the future and forecasts suggest that people visiting and living at the baltic sea are at particular risk. this study aimed to investigate antimicrobial resistance patterns among vibrio vulnificus and vibrio cholerae non-o1/non-o139 isolates that could pose a public health ris ... | 2015 | 26579088 |
| transgenic expression of omega-3 pufa synthesis genes improves zebrafish survival during vibrio vulnificus infection. | highly desaturated n-3 polyunsaturated fatty acids (pufas), such as eicosapentaenoic acid (epa) and docosahexaenoic acid (dha), are synthesized by desaturases and elongase. they exert hepatoprotective effects to prevent alcoholic fatty liver syndrome or cholestatic liver injury. however, it is unclear how n-3 pufas improve immune function in liver. vibrio vulnificus, a gram-negative bacterial pathogen, causes high mortality of aquaculture fishes upon infection. humans can become infected with v. ... | 2015 | 26572495 |
| prevalence and antimicrobial resistance of vibrio spp. in retail and farm shrimps in ecuador. | the aim of this study was to investigate the prevalence of vibrio spp. in shrimp at retail and in shrimp farms in ecuador and to determine the antimicrobial agent resistance patterns of farm isolates. the presence of genes linked to early mortality syndrome (ems) or acute hepatopancreatic necrosis disease (ahpnd) also was evaluated. vibrio spp. were isolated from retail shrimps in cuenca, ecuador, and farm shrimps originating from provinces el oro and guayas, ecuador. a total of 229 shrimp sampl ... | 2015 | 26555534 |
| vvpe mediates the intestinal colonization of vibrio vulnificus by the disruption of tight junctions. | the disruption of gastrointestinal tight junctions and their colonization evoked by enteric pathogens are hallmarks of the pathogenesis. vibrio (v.) vulnificus, vvpe, is an elastase which is responsible for host surface adherence and vascular permeability; however, the functional roles of vvpe in the pathogenesis of v. vulnificus (wt) are poorly understood. in the present study, we have investigated the role of vvpe in regulation of intestinal tight junctions and the colonization of wt. we found ... | 2016 | 26552364 |
| detection and antimicrobial resistance of vibrio isolates in aquaculture environments: implications for public health. | the aim of this study was to evaluate the presence of vibrio isolates recovered from four different fish pond facilities in benin city, nigeria, determine their antibiogram profiles, and evaluate the public health implications of these findings. fish pond water samples were collected from four sampling sites between march and september 2014. a total of 56 samples were collected and screened for the isolation of vibrio species using standard culture-based methods. polymerase chain reaction (pcr) ... | 2016 | 26540391 |
| to notify or not to notify: decision aid for policy makers on whether to make an infectious disease mandatorily notifiable. | mandatory notification can be a useful tool to support infectious disease prevention and control. guidelines are needed to help policymakers decide whether mandatory notification of an infectious disease is appropriate. we developed a decision aid, based on a range of criteria previously used in the netherlands or in other regions to help decide whether to make a disease notifiable. criteria were categorised as being effective, feasible and necessary with regard to the relevance of mandatory not ... | 2015 | 26530302 |
| a specific antimicrobial protein cap-1 from pseudomonas sp. isolated from the jellyfish cyanea capillata. | a bacterium strain, designated as cmf-2, was isolated from the jellyfish cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. the strain cmf-2 was identified as pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16s rrna sequence analysis. in this study, an antimicrobial protein, named as cap-1, was isolated from the culture of cmf-2 through ammonium sulfate precipitation and gel filtration chromatography. ... | 2016 | 26529191 |
| all three tonb systems are required for vibrio vulnificus cmcp6 tissue invasiveness by controlling flagellum expression. | tonb systems actively transport iron-bound substrates across the outer membranes of gram-negative bacteria. vibrio vulnificus cmcp6, which causes fatal septicemia and necrotizing wound infections, possesses three active tonb systems. it is not known why v. vulnificus cmcp6 has maintained three tonb systems throughout its evolution. the tonb1 and tonb2 systems are relatively well characterized, while the pathophysiological function of the tonb3 system is still elusive. a reverse transcription-pcr ... | 2015 | 26527216 |
| vibrio vulnificus necrotizing fasciitis associated with acupuncture. | necrotizing fasciitis is a severe life-threatening infection of the deep subcutaneous tissues and fascia. infection with vibrio vulnificus, a halophilic gram-negative bacillus found worldwide in warm coastal waters, can lead to severe complications, particularly among patients with chronic liver diseases. we herein present an unusual case of necrotizing fasciitis caused by v. vulnificus triggered by acupuncture needle insertion. the patient, who suffered from diabetes mellitus and nonalcoholic f ... | 2015 | 26500738 |
| correction: the cababc operon essential for biofilm and rugose colony development in vibrio vulnificus. | [this corrects the article doi: 10.1371/journal.ppat.1005192.]. | 2015 | 26496079 |
| antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro. | neem (azadirachta indica) is recognized as a medicinal plant well known for its antibacterial, antimalarial, antiviral, and antifungal properties. neem nanoemulsion (ne) (o/w) is formulated using neem oil, tween 20, and water by high-energy ultrasonication. the formulated neem ne showed antibacterial activity against the bacterial pathogen vibrio vulnificus by disrupting the integrity of the bacterial cell membrane. despite the use of neem ne in various biomedical applications, the toxicity stud ... | 2015 | 26491309 |
| draft genome sequence of the pathogenic bacterium vibrio vulnificus v252 biotype 1, isolated in israel. | we report the genome sequence of the pathogenic vibrio vulnificus biotype 1 clade b, which is suggested to have a common ancestor with biotype 3. this draft genome of the clinical strain v252, isolated in israel, represents the clonal clade b group that contains both clinical and environmental strains. | 2015 | 26472833 |
| severe septicemia, necrotizing fasciitis, and peritonitis due to vibrio vulnificus in a patient undergoing continuous ambulatory peritoneal dialysis: a case report. | chronic kidney disease, including end-stage renal disease, has been identified as a possible risk factor for primary septicemia and wound infection by vibrio vulnificus. however, cases of severe septicemia, necrotizing fasciitis, and peritonitis caused by v. vulnificus in patients undergoing continuous ambulatory peritoneal dialysis (capd) have not been described. we report a case of severe septicemia, necrotizing fasciitis, and peritonitis due to v. vulnificus in a patient undergoing capd after ... | 2015 | 26467000 |
| erratum to: identification of the vibrio vulnificus htpg gene and its influence on cold shock recovery. | 2015 | 26428926 | |
| presence of nitric oxide-sensing systems in the human pathogen vibrio vulnificus. | vibrio vulnificus is a halophilic estuarine bacterium, but this species causes fatal septicemia in humans. v. vulnificus may encounter many kinds of stresses either in the natural environment or in the human body. one of the striking stresses is the exposure to the reactive oxygen species including nitric oxide (no). the present study revealed that no could participate in the regulation of the v. vulnificus community behavior. when the bacterium was cultivated in the presence of sub-lethal doses ... | 2015 | 26412700 |
| the cababc operon essential for biofilm and rugose colony development in vibrio vulnificus. | a transcriptome analysis identified vibrio vulnificus cababc genes which were preferentially expressed in biofilms. the cababc genes were transcribed as a single operon. the caba gene was induced by elevated 3',5'-cyclic diguanylic acid (c-di-gmp) and encoded a calcium-binding protein caba. comparison of the biofilms produced by the caba mutant and its parent strain jn111 in microtiter plates using crystal-violet staining demonstrated that caba contributed to biofilm formation in a calcium-depen ... | 2015 | 26406498 |
| safety and vaccine efficacy of an attenuated vibrio vulnificus strain with deletions in major cytotoxin genes. | vibrio vulnificus is a human pathogen causing a rapidly progressing fatal septicemia. we have previously reported that a v. vulnificus large toxin rtxa1 causes programmed necrotic cell death through calcium-mediated mitochondrial dysfunction. here we developed a live attenuated vaccine strain (cmm781) having deletions in three genes encoding major virulence factors: rtx cytotoxin (rtxa1), hemolysin/cytolysin (vvha) and metalloprotease (vvpe) of a clinical isolate strain cmcp6. the cmm781 strain ... | 2015 | 26381905 |
| clinical and environmental genotypes of vibrio vulnificus display distinct, quorum-sensing-mediated, chitin detachment dynamics. | the ability for bacteria to attach to and detach from various substrata is important for colonization, survival and transitioning to new environments. an opportunistic human pathogen, vibrio vulnificus, can cause potentially fatal septicemia after ingestion of undercooked seafood. based on genetic polymorphisms, strains of this species are subtyped into clinical (c) and environmental (e) genotypes. vibrio vulnificus readily associates with chitin, thus we investigated chitin detachment dynamics ... | 2015 | 26377182 |
| the makes caterpillars floppy (mcf)-like domain of vibrio vulnificus induces mitochondrion-mediated apoptosis. | the multifunctional-autoprocessing repeats-in-toxin (martxvv) toxin of vibrio vulnificus plays a significant role in the pathogenesis of this bacterium through delivery of up to five effector domains to the host cells. previous studies have established that the martxvv toxin is linked to v. vulnificus dependent induction of apoptosis, but the region of the large multifunction protein essential for this activity was not previously identified. recently, we showed that the makes caterpillar floppy- ... | 2015 | 26351282 |
| rapid proliferation of vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae during freshwater flash floods in french mediterranean coastal lagoons. | vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae of the non-o1/non-o139 serotype are present in coastal lagoons of southern france. in these mediterranean regions, the rivers have long low-flow periods followed by short-duration or flash floods during and after heavy intense rainstorms, particularly at the end of the summer and in autumn. these floods bring large volumes of freshwater into the lagoons, reducing their salinity. water temperatures recorded during sampling (15 to 24° ... | 2015 | 26319881 |
| hydrogen peroxide causes vibrio vulnificus bacteriolysis accelerated by sulfonyl fluoride compounds. | induction of bacteriolysis of vibrio vulnificus cells by 10 mm hydrogen peroxide (h(2)o(2)) was analyzed. all vibrio species examined, except for vibrio hollisae, were lysed by 10 mm h(2)o(2). bacteriophage induction was not the cause of h(2)o(2)-induced bacteriolysis. autolysis is also known to cause bacteriolysis. vvps protein is a serine protease of v. vulnificus essential for autolysis. vvps mutant underwent h(2)o(2)-induced bacteriolysis in the same manner as the wild type. protease inhibit ... | 2015 | 26316164 |
| flagellin suppresses experimental asthma by generating regulatory dendritic cells and t cells. | although the hygiene hypothesis suggests that microbial infections could subvert asthma and thus a microbial product might serve as a therapeutic adjuvant for asthma, the relationship between bacterial components and asthma is complex. recently, low levels of flagellin, the toll-like receptor (tlr) 5 ligand, have been reported to promote asthma. | 2016 | 26303344 |
| assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis. | colonization of the squid euprymna scolopes by vibrio fischeri requires biofilm formation dependent on the 18-gene symbiosis polysaccharide locus, syp. one key regulator, sypa, controls biofilm formation by an as-yet unknown mechanism; however, it is known that sypa itself is regulated by sype. biofilm-proficient strains form wrinkled colonies on solid media, while sypa mutants form biofilm-defective smooth colonies. to begin to understand the function of sypa, we used comparative analyses and m ... | 2015 | 26284045 |
| correlations between clinical features and mortality in patients with vibrio vulnificus infection. | vibrio vulnificus is a common gram-negative bacterium, which might cause morbidity and mortality in patients following consumption of seafood or exposure to seawater in southeast china. we retrospectively analyzed clinical data of patients with laboratory confirmed v. vulnificus infection. twenty one patients were divided into a survival group and a non-surviving (or death) group according to their clinical outcome. clinical data and measurements were statistically analyzed. four patients (19.05 ... | 2015 | 26274504 |
| expression, purification, crystallization and x-ray crystallographic analysis of the periplasmic binding protein vatd from vibrio vulnificus m2799. | vibrio vulnificus is a halophilic marine microorganism which causes gastroenteritis and primary septicaemia in humans. an important factor that determines the survival of v. vulnificus in the human body is its ability to acquire iron. vatd is a periplasmic siderophore-binding protein from v. vulnificus m2799. the current study reports the expression, purification and crystallization of vatd. crystals of both apo vatd and a vatd-desferrioxamine b-fe(3+) (vatd-fob) complex were obtained. the cryst ... | 2015 | 26249703 |
| actin-directed toxin. acd toxin-produced actin oligomers poison formin-controlled actin polymerization. | the actin cross-linking domain (acd) is an actin-specific toxin produced by several pathogens, including life-threatening spp. of vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. actin cross-linking by acd is thought to lead to slow cytoskeleton failure owing to a gradual sequestration of actin in the form of nonfunctional oligomers. here, we found that acd converted cytoplasmic actin into highly toxic oligomers that potently "poisoned" the ability of major actin assembly proteins, ... | 2015 | 26228148 |
| vibrio vulnificus vvpe stimulates il-1β production by the hypomethylation of the il-1β promoter and nf-κb activation via lipid raft-dependent anxa2 recruitment and reactive oxygen species signaling in intestinal epithelial cells. | an inflammatory response is a hallmark of necrosis evoked by bacterial pathogens. vibrio vulnificus, vvpe, is an elastase that is responsible for tissue necrosis and inflammation; however, the molecular mechanism by which it regulates host cell death has not been characterized. in the present study, we investigate the cellular mechanism of vvpe with regard to host cell death and the inflammatory response of human intestinal epithelial (int-407) cells. the recombinant protein (r)vvpe (50 pg/ml) c ... | 2015 | 26223656 |
| effects of dry storage and resubmersion of oysters on total vibrio vulnificus and total and pathogenic (tdh+/trh+) vibrio parahaemolyticus levels. | vibrio vulnificus (vv) and vibrio parahaemolyticus (vp) are the two leading causes of bacterial illnesses associated with raw shellfish consumption. levels of these pathogens in oysters can increase during routine antifouling aquaculture practices involving dry storage in ambient air conditions. after storage, common practice is to resubmerge these stored oysters to reduce elevated vv and vp levels, but evidence proving the effectiveness of this practice is lacking. this study examined the chang ... | 2015 | 26219373 |
| an enriched european eel transcriptome sheds light upon host-pathogen interactions with vibrio vulnificus. | infectious diseases are one of the principal bottlenecks for the european eel recovery. the aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. to this end, we first stimulated adult eels with different pathogen-associated molecular patterns (pamps), extracted rna from the immune-related tissues and sequenced the transcriptome. we obtained more than 2 x 10(6) reads that were assembled and annotated into 45,067 new descriptions wit ... | 2015 | 26207370 |
| draft genome sequence of environmental bacterium vibrio vulnificus cladea-yb158. | we report the genome sequence of the environmental vibrio vulnificus biotype 1_cladea. this draft genome of the cladea-yb158 strain, isolated in israel, represents this newly emerged clonal group that contains both clinical and environmental strains. | 2015 | 26205875 |
| evaluation of ice slurries as a control for postharvest growth of vibrio spp. in oysters and potential for filth contamination. | raw oyster consumption is the most common route of exposure for vibrio spp. infections in humans. vibriosis has been increasing steadily in the united states despite efforts to reduce the incidence of the disease. research has demonstrated that ice is effective in reducing postharvest vibrio spp. growth in oysters but has raised concerns of possible contamination of oyster meat by filth (as indicated by the presence of fecal coliform bacteria or clostridium perfringens). this study examined the ... | 2015 | 26197291 |
| the biology of vibrio vulnificus. | vibrio vulnificus, carrying a 50% fatality rate, is the most deadly of the foodborne pathogens. it occurs in estuarine and coastal waters and it is found in especially high numbers in oysters and other molluscan shellfish. the biology of v. vulnificus, including its ecology, pathogenesis, and molecular genetics, has been described in numerous reviews. this article provides a brief summary of some of the key aspects of this important human pathogen, including information on biotypes and genotypes ... | 2015 | 26185084 |