Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| in vitro study of possible microbial indicators for drowning: salinity and types of bacterioplankton proliferating in blood. | numbers and types of bacterioplankton proliferating in blood samples mixed with water of various salinity levels were examined to determine the characteristics of species associated with salinity. water samples (total n=88) were collected from the midstream of two rivers (freshwater; n=10; salinity <0.05%), from around their estuaries (areas of freshwater, n=20, salinity <0.05%; areas of brackish water, n=20, salinity <0.05-3.1%; areas of marine water beyond the mouths of the rivers, n=28, salin ... | 2011 | 20554407 |
| resurgence of morbillivirus infection in mediterranean dolphins off the french coast. | 2010 | 20495168 | |
| abundance, diversity, and activity of microbial assemblages associated with coral reef fish guts and feces. | feces and distal gut contents were collected from three coral reef fish species. bacteria cell abundances, as determined via epifluorescence microscopy, ranged two orders of magnitude among the fishes. mass-specific and apparent cell-specific hydrolytic enzyme activities in feces from chlorurus sordidus were very high, suggesting that endogenous fish enzymes were egested into feces. denaturing gradient gel electrophoresis profiles of 16s rrna genes were more similar among multiple individuals of ... | 2010 | 20455942 |
| vaccination trials of sea bass (dicentrarchus labrax) against pasteurellosis using oral, intraperitoneal and immersion methods. | photobacterium damsela subsp. piscicida (phdp) is the aetiological agent of fish pasteurellosis, causing heavy losses in intensive mariculture plants. the present work compares the protective efficacy of five different vaccine formulation: oral, intraperitoneal, immersion, bivalent immersion (vibrio anguillarum) and immersion associated with immunostimulants. each of these vaccine formulations containing whole cells of phdp formalin inactivated (fkc), was administered to 100 sea bass weighing ap ... | 2005 | 20437375 |
| enzymatic synthesis of unique sialyloligosaccharides using marine bacterial alpha-(2-->3)- and alpha-(2-->6)-sialyltransferases. | we investigated the acceptor substrate specificities of marine bacterial alpha-(2-->3)-sialyltransferase cloned from photobacterium sp. jt-ish-224 and alpha-(2-->6)-sialyltransferase cloned from photobacterium damselae jt0160 using several saccharides as acceptor substrates. after purifying the enzymatic reaction products, we confirmed their structure by nmr spectroscopy. the alpha-(2-->3)-sialyltransferase transferred n-acetylneuraminic acid (neu5ac) from cytidine 5'-monophospho-n-acetylneurami ... | 2010 | 20413108 |
| [evaluation of the phagocytic activity of human peripheral blood neutrophils, by employing recombinant luminescent bacteria]. | the authors show it possible to evaluate the phagocytic activity of human peripheral blood neutrophils from their impact on the fluorescence intensity of recombinant luminescent bacteria. they have established the preferred use of recombinant luminescent escherichia coli bacteria with cloned luxcdabe genes of photobacterium leiognathii as phagocyted objects and substantiated the optimal method of their opsonization by normal human immunoglobulin. the variants of separate consideration of the lum ... | 2010 | 20401994 |
| identification and cloning of a selenium-dependent glutathione peroxidase from tiger shrimp, penaeus monodon, and its transcription following pathogen infection and related to the molt stages. | complementary (c)dna encoding glutathione peroxidase (gpx) messenger (m)rna of the tiger shrimp penaeus monodon was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) method. the 1321-bp cdna contained an open reading frame (orf) of 564bp, a 69-bp 5'-untranslated region (utr), and a 688-bp 3'-utr containing a poly a tail and a conserved selenocysteine insertion sequence (secis) element. the molecular mass of the dedu ... | 2010 | 20399225 |
| development of molecular-based methods for determination of high histamine producing bacteria in fish. | histamine (or scombroid) fish poisoning is a significant cause of food borne disease in the united states. in this study, we describe the development of a molecular-based technique which uses digoxigenin (dig) labeled dna probes for the detection of gram negative bacteria producing high amounts of histamine (>1000 ppm). a cocktail of pcr amplification fragments corresponding to a 709 bp fragment of the histidine decarboxylase (hdc) gene of four high producing bacteria (morganella morganii, enter ... | 2010 | 20392504 |
| comparisons of vibrio fischeri, photobacterium phosphoreum, and recombinant luminescent using escherichia coli as bod measurement. | to shorten the time needed to measure biochemical oxygen demand (bod) in water samples and to provide a rapid feedback of the real condition of water quality, we tested and evaluated the validity and reliability of luminescent bacteria vibrio fischeri, photobacterium phosphoreum, and recombinant escherichia coli as potential indicators of bod in the domestic wastewaters. the results indicate that the luminescence intensities of these strains are dependent on temperature, ph, and bod concentratio ... | 2010 | 20390863 |
| insights into piezophily from genetic studies on the deep-sea bacterium, photobacterium profundum ss9. | the deep-sea bacterium, photobacterium profundum ss9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. despite growing optimally at 28 mpa (15 degrees c), p. profundum ss9 can grow over a wide range of pressures and temperatures. the ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in p. pr ... | 2010 | 20233381 |
| efficient synthesis of 6'-sialyllactose, 6,6'-disialyllactose, and 6'-kdo-lactose by metabolically engineered e. coli expressing a multifunctional sialyltransferase from the photobacterium sp. jt-ish-224. | we have previously reported the efficient conversion of lactose into 3'-sialyllactose by high cell density cultures of a genetically engineered escherichia coli strain expressing the neisseria meningitidis gene for alpha-(2-->3)-sialyltransferase [fierfort, n.; samain, e. j. biotechnol. 2008, 134, 261-265.]. first attempts to use a similar strategy to produce 6'-sialyllactose with a strain expressing alpha-(2-->6)-sialyltransferase from the photobacterium sp. jt-ish-224 led to the production of ... | 2010 | 20231015 |
| photobacterium swingsii sp. nov., isolated from marine organisms. | six gram-negative coccobacilli were isolated from pacific oysters (crassostrea gigas) from mexico and haemolymph of spider crabs (maja brachydactyla) from spain. all of the isolates grew as small green colonies on thiosulphate-citrate-bile salts-sucrose (tcbs) agar and were facultatively anaerobic, oxidase-positive and sensitive to the vibriostatic agent o/129. repetitive palindromic pcr analysis revealed a high degree of genomic homogeneity among the isolates. several phenotypic traits differen ... | 2011 | 20228205 |
| molecular cloning of sea bass (dicentrarchus labrax l.) caspase-8 gene and its involvement in photobacterium damselae ssp. piscicida triggered apoptosis. | caspase-8 is an initiator caspase that plays a crucial role in some cases of apoptosis by extrinsic and intrinsic pathways. caspase-8 structure and function have been extensively studied in mammals, but in fish the characterization of that initiator caspase is still scarce. in this work, the sea bass counterpart of mammalian caspase-8 was sequenced and characterized, and its involvement in the apoptogenic activity of a toxin from a fish pathogen was assessed. a 2472 bp cdna of sea bass caspase-8 ... | 2010 | 20202478 |
| [growth and bioluminescence of luminous bacteria under the action of aflatoxin b1 before and after its treatment with nanodiamonds]. | the effect of aflatoxin b1 on growth and luminescence of marine luminous bacteria p. phosphoreum and recombinant e. coli z905 cells was investigated. the bidirectional effect of aflatoxin b1 on the studied bacterial species was detected--an inhibition of luminescence in p. phosphoreum and its stimulation in e. coli. it was shown that aflatoxin b1 influences the cell luminescence in the freshly grown cultures and bacteria restored after lyophilization. it was detected that the effect of aflatoxin ... | 2010 | 20198915 |
| [characteristics of the response of natural and recombinant luminescent microorganisms in the presence of fe2+ ions]. | it was found that divalent iron ions have alternative effects on the bioluminescence of the natural marine microorganism photobacterium phosphoreum and the recombinant escherichia coli strain with a cloned lux operon of p. leiognathi. in the presence of 0.25-5.0 mm feso4, the bioluminescence intensity of the former and the latter increased and decreased, respectively. to establish the causes of these differences, we studied the characteristics of the fatty acid composition of the compared microo ... | 2010 | 20198914 |
| differential characterization of biogenic amine-producing bacteria involved in food poisoning using maldi-tof mass fingerprinting. | histamine poisoning is caused by the consumption of fish and other foods that harbor bacteria possessing histidine decarboxylase activity. with the aim of preventing histamine formation, highly specific mass spectral fingerprints were obtained from the 16 major biogenic amine-producing enteric and marine bacteria by means of maldi-tof ms analysis. all bacterial strains analyzed exhibited specific spectral fingerprints that enabled its unambiguous differentiation. this technique also identified p ... | 2010 | 20151397 |
| establishment and characterization of a fibroblast cell line derived from the dorsal fin of red sea bream, pagrus major (temminck & schlegel). | the establishment and partial characterization of a continuous cell line from the dorsal fin of red sea bream, pagrus major, are described. the cell line, designated rsbf-2, has been subcultured for more than 100 passages since its initiation in november 2000. it was optimally maintained at 28 degrees c in leibovitz l-15 medium with 10% foetal bovine serum. propagation of rsbf-2 cells was serum dependent and exhibited low plating efficiency (<1.7%). aside from long-term cryopreservation, the cel ... | 2010 | 20102463 |
| catabolite regulation of enzymatic activities in a white pox pathogen and commensal bacteria during growth on mucus polymers from the coral acropora palmata. | colonization of host mucus surfaces is one of the first steps in the establishment of coral-associated microbial communities. coral mucus contains a sulfated glycoprotein (in which oligosaccharide decorations are connected to the polypeptide backbone by a mannose residue) and molecules that result from its degradation. mucus is utilized as a growth substrate by commensal and pathogenic organisms. two representative coral commensals, photobacterium mandapamensis and halomonas meridiana, differed ... | 2009 | 20095241 |
| photobacterium jeanii sp. nov., isolated from corals and zoanthids. | four novel isolates (r-40508(t), r-40507, r-40903 and r-21419) were obtained from different cnidarian species (phyllogorgia dilatata, merulina ampliata and palythoa caribaeorum) from different places in brazil and australia. the novel isolates formed a tight phylogenetic group based on 16s rrna, reca, topa, ftsz, mreb and rpoa gene sequences. their closest phylogenetic neighbours were the type strains of photobacterium leiognathi, p. rosenbergii and p. halotolerans, sharing 97.1-97.5 % 16s rrna ... | 2010 | 20081016 |
| [effects of antibiotics on bacterial community in shrimp hatchery system]. | with bacterial 16s rrna gene (rdna) as molecular marker and by using pcr-dgge technique, the fingerprints of bacterial community were constructed to study the effects of applying streptomycin sulfate, terramycin, and penicillin on the bacterial community in shrimp hatchery system. within the 120 h experimental period, significant difference in the diversity of the bacterial community was observed between the treatments applied with 0.5 mg x l(-1) of test antibiotics and the control. in the contr ... | 2009 | 20077718 |
| degradation of diazinon in apple juice by ultrasonic treatment. | the degradation of diazinon spiked in apple juice treated by ultrasonic treatment was investigated in this paper. results showed that the ultrasonic power and initial concentration of diazinon significantly influenced the degradation percentage of diazinon (p<0.05) and the degradation of diazinon followed the first-order kinetics model well. based on the gas chromatography-mass spectrometry (gc-ms) analysis, seven degradation products of diazinon have been identified. a degradation pathway invol ... | 2010 | 20061173 |
| rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry. | vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ... | 2010 | 20059616 |
| rapid determination of the toxicity of quantum dots with luminous bacteria. | in this paper, a novel method so-called bioluminescence inhibition assay with luminous bacteria (photobacterium phosphoreum) was introduced to evaluate the toxicity of quantum dots. the bioassay was based on measuring the decrease of the light emitted by luminous bacteria. with obvious advantages of simplicity, rapidity and sensitivity, it can dramatically improve the efficiency of probing the toxicity of qds. based on this method, we systemically explored the effect of the composition and surfa ... | 2010 | 20056317 |
| evaluation of combined toxicity of phenols and lead to photobacterium phosphoreum and quantitative structure-activity relationships. | the combined toxicity of lead (pb) and nine phenols were measured. the result indicated that the combined toxicity is not only dependent on the pb concentrations but also on the positions of substituted groups of phenols. quantitative structure-activity relationship equations were built from the combined toxicity and physico-chemical descriptors of phenols in the different pb concentrations. the combined toxicity was related to water solubility and the third order molecular connectivity index (( ... | 2010 | 20043147 |
| cloning and characterization of dihydrofolate reductases from deep-sea bacteria. | enzymes from organisms living in deep-sea are thought to have characteristic pressure-adaptation mechanisms in structure and function. to better understand these mechanisms in dihydrofolate reductase (dhfr), an essential enzyme in living cells, we cloned, overexpressed and purified four new dhfrs from the deep-sea bacteria shewanella violacea (svdhfr), photobacterium profundum (ppdhfr), moritella yayanosii (mydhfr) and moritella japonica (mjdhfr), and compared their structure and function with t ... | 2010 | 20040594 |
| [quantitative detection of nadh by in vitro bacterial luciferase bioluminescent]. | the study aimed at establishing the bacterial luciferase: fmn-nadh oxidoreductase bioluminescent system in vitro and evaluating its potential for quantitative detection of nadh. | 2009 | 20030062 |
| photobacterium gaetbulicola sp. nov., a lipolytic bacterium isolated from a tidal flat sediment. | a gram-negative, motile, non-spore-forming and lipolytic bacterial strain, designated gung47(t), was isolated from a tidal flat on the west coast of korea. strain gung47(t) grew optimally at 30 °c and with 2-5 % (w/v) nacl. phylogenetic analyses based on 16s rrna gene sequences revealed that strain gung47(t) belonged to the genus photobacterium. strain gung47(t) exhibited 98.1 % 16s rrna gene sequence similarity with photobacterium rosenbergii lmg 22223(t) and 94.3-96.3 % similarity with other t ... | 2010 | 20023056 |
| mechanism of the synergistic toxicity of malononitrile and p -nitrobenzaldehyde with photobacterium phosphoreum. | in this study the mechanism of synergistic toxicity between malononitrile and p -nitrobenzaldehyde was revealed by examining their toxicodynamic and toxicokinetic interactions. the investigation of the toxicodynamic interactions focused on the interactions among malononitrile, p -nitrobenzaldehyde, and luciferase, and found that malononitrile does not induce luciferase to bind p -nitrobenzaldehyde. this result suggested that the synergistic toxicity does not arise from toxicodynamic interactions ... | 2003 | 20021148 |
| septicemia progressing to fatal hepatic dysfunction in an cirrhotic patient after oral ingestion of photobacterium damsela: a case report. | 2009 | 20013095 | |
| bacterial composition and succession during storage of north-atlantic cod (gadus morhua) at superchilled temperatures. | the bacteriology during storage of the north-atlantic cod has been investigated for the past decades using conventional cultivation strategies which have generated large amount of information. this paper presents a study where both conventional cultivation and cultivation independent approaches were used to investigate the bacterial succession during storage of cod loins at chilled and superchilled temperatures. | 2009 | 19961579 |
| a new effect residual ratio (err) method for the validation of the concentration addition and independent action models. | glutaraldehyde (ga) often acts as an effective sterilant, disinfectant, and preservative in chemical products. it was found that ga had clearly acute toxicity to aquatic organisms. furthermore, ga in natural environment could not exist as single species but as complex mixtures. to explore the toxicity interaction between ga and the other environmental pollutant, it is necessary to determine the mixture toxicities of various binary mixtures including ga. two reference models, concentration additi ... | 2010 | 19949878 |
| blacmy-2-positive inca/c plasmids from escherichia coli and salmonella enterica are a distinct component of a larger lineage of plasmids. | large multidrug resistance plasmids of the a/c incompatibility complex (inca/c) have been found in a diverse group of gram-negative commensal and pathogenic bacteria. we present three completed sequences from inca/c plasmids that originated from escherichia coli (cattle) and salmonella enterica serovar newport (human) and that carry the cephamycinase gene blacmy-2. these large plasmids (148 to 166 kbp) share extensive sequence identity and synteny. the most divergent plasmid, peh4h, has lost sev ... | 2010 | 19949054 |
| development of a haemolysin gene-based multiplex pcr for simultaneous detection of vibrio campbellii, vibrio harveyi and vibrio parahaemolyticus. | to develop a haemolysin (hly) gene-based species-specific multiplex pcr for simple and rapid detection of vibrio campbellii, v. harveyi and v. parahaemolyticus. | 2010 | 19943887 |
| cloning and sequencing of alginate lyase genes from deep-sea strains of vibrio and agarivorans and characterization of a new vibrio enzyme. | four alginate lyase genes were cloned and sequenced from the genomic dnas of deep-sea bacteria, namely members of vibrio and agarivorans. three of them were from vibrio sp. jam-a9m, which encoded alginate lyases, a9mt, a9mc, and a9ml. a9mt was composed of 286 amino acids and 57% homologous to alxm of photobacterium sp. a9mc (221 amino acids) and a9ml (522 amino acids) had the highest degree of similarity to two individual alginate lyases of vibrio splendidus with 74% and 84% identity, respective ... | 2010 | 19941025 |
| molecular intraspecific characterization of photobacterium damselae ssp. damselae strains affecting cultured marine fish. | the aim of this study was to analyse the intraspecific variability of photobacterium damselae ssp. damselae strains isolated from different cultured marine fish species using molecular typing methods. | 2010 | 19922594 |
| synthesis, cytotoxicity by bioluminescence inhibition, antibacterial and antifungal activity of ([1,2,4]triazolo[1,5-c]quinazolin-2-ylthio)carboxylic acid amides. | we report in this work the synthesis, cytotoxicity, and antimicrobial activity of ([1,2,4]triazolo[1,5-c]quinazolin-2-ylthio)carboxylic acid amides 4-7 in connection with our previous research in the preparation of triazoloquinazoline derivatives. due to simplicity, general availability of starting materials, and high yields, the most reliable method of synthesis appeared to be the one with n,n-carbonyldiimidazole activation stage. the chemical structures of all obtained substances were deduced ... | 2009 | 19882690 |
| trans-sialidase activity of photobacterium damsela alpha2,6-sialyltransferase and its application in the synthesis of sialosides. | trans-sialidases catalyze the transfer of a sialic acid from one sialoside to an acceptor to form a new sialoside. alpha2,3-trans-sialidase activity was initially discovered in the parasitic protozoan trypanosoma cruzi, and more recently was found in a multifunctional pasteurella multocida sialyltransferase pmst1. alpha2,8-trans-sialidase activity was also described for a multifunctional campylobacter jejuni sialyltransferase cstii. we report here the discovery of the alpha2,6-trans-sialidase ac ... | 2010 | 19880425 |
| [characterization of listonella anguillarum as the aetiological agent of vibriosis occurred in cultured ayu (plecoglossus altivelis) in ninghai country, china]. | ayu (plecoglossus altivelis) vibriosis threatens ayu aquaculture seriously caused by mass mortality due to severe infections. we characterized the vibriosis pathogen of ayu in ninghai country. | 2009 | 19873758 |
| the nature and control of reactions in bioluminescence : with special reference to the mechanism of reversible and irreversible inhibitions by hydrogen and hydroxyl ions, temperature, pressure, alcohol, urethane, and sulfanilamide in bacteria. | on the basis of available data with regard to the chemical and physical properties of the "substrate" luciferin (lh(2)) and enzyme, luciferase (a), and of kinetic data derived both from the reaction in extracts of cypridina, and from the luminescence of intact bacteria, the fundamental reactions involved in the phenomenon of bioluminescence have been schematized. these reactions provide a satisfactory basis for interpreting the known characteristics of the system, as well as the theoretical chem ... | 1945 | 19873433 |
| genomic taxonomy of vibrios. | vibrio taxonomy has been based on a polyphasic approach. in this study, we retrieve useful taxonomic information (i.e. data that can be used to distinguish different taxonomic levels, such as species and genera) from 32 genome sequences of different vibrio species. we use a variety of tools to explore the taxonomic relationship between the sequenced genomes, including multilocus sequence analysis (mlsa), supertrees, average amino acid identity (aai), genomic signatures, and genome blast atlases. ... | 2009 | 19860885 |
| crystal structures of the lumazine protein from photobacterium kishitanii in complexes with the authentic chromophore, 6,7-dimethyl- 8-(1'-d-ribityl) lumazine, and its analogues, riboflavin and flavin mononucleotide, at high resolution. | lumazine protein (lump) is a fluorescent accessory protein having 6,7-dimethyl-8-(1'-d-ribityl) lumazine (dmrl) as its authentic chromophore. it modulates the emission of bacterial luciferase to shorter wavelengths with increasing luminous strength. to obtain structural information on the native structure as well as the interaction with bacterial luciferase, we have determined the crystal structures of lump from photobacterium kishitanii in complexes with dmrl and its analogues, riboflavin (rbf) ... | 2010 | 19854891 |
| sialyltransferases of marine bacteria efficiently utilize glycosphingolipid substrates. | bacterial sialyltransferases (sts) from marine sources were characterized using glycosphingolipids (gsls). bacterial sts were found to be beta-galacotoside sts. there were two types of sts: (1) st obtained from strains such as ishi-224, 05jtc1 (#1), ishi-467, 05jtd2 (#2), and faj-16, 05jte1 (#3), which form alpha2-3 sialic acid (sia) linkages, named alpha2-3st, (2) st obtained from strains such as ish-224, n1c0 (#4), pda-rec, 05jtb2 (#5), and pda-0160, 05jta2 (#6), which form alpha2-6 sia linkag ... | 2010 | 19837852 |
| the genomic basis of trophic strategy in marine bacteria. | many marine bacteria have evolved to grow optimally at either high (copiotrophic) or low (oligotrophic) nutrient concentrations, enabling different species to colonize distinct trophic habitats in the oceans. here, we compare the genome sequences of two bacteria, photobacterium angustum s14 and sphingopyxis alaskensis rb2256, that serve as useful model organisms for copiotrophic and oligotrophic modes of life and specifically relate the genomic features to trophic strategy for these organisms an ... | 2009 | 19805210 |
| an alpha2,6-sialyltransferase cloned from photobacterium leiognathi strain jt-shiz-119 shows both sialyltransferase and neuraminidase activity. | we cloned, expressed, and characterized a novel beta-galactoside alpha2,6-sialyltransferase from photobacterium leiognathi strain jt-shiz-119. the protein showed 56-96% identity to the marine bacterial alpha2,6-sialyltransferases classified into glycosyltransferase family 80. the sialyltransferase activity of the n-terminal truncated form of the recombinant enzyme was 1477 u/l of escherichia coli culture. the truncated recombinant enzyme was purified as a single band by sodium dodecyl sulfate po ... | 2010 | 19797322 |
| hormesis response of marine and freshwater luminescent bacteria to metal exposure. | the stimulatory effect of low concentrations of toxic chemicals on organismal metabolism, referred to as hormesis, has been found to be common in the widely used luminescence bioassay. this paper aims to study the hormesis phenomenon in both marine and freshwater luminescent bacteria, named photobacterium phosphorem and vibrio qinghaiensis. the effects of cu (ii), zn (ii), cd (ii) and cr (vi) on luminescence of these two bacteria were studied for 0 to 75 minutes exposure by establishing dose- an ... | 2009 | 19746263 |
| effect of brining, modified atmosphere packaging, and superchilling on the shelf life of cod (gadus morhua) loins. | the aim of these experiments was to evaluate the effect of brining, modified atmosphere packaging (map), and superchilling on the quality changes of cod loins as measured by microbial, sensory, and chemical analysis. unbrined and brined (2.5 +/- 1.0% nacl) cod loins were kept in styrofoam boxes (air) and under modified atmosphere (ma, co(2)/o(2)/n(2): 50/5/45) at 0, -2, and -3.6 degrees c. samples were examined over a 4-wk period. total viable psychrotrophic counts and counts of h(2)s-producing ... | 2009 | 19723210 |
| quality changes of prerigor filleted atlantic salmon (salmo salar l.) packaged in modified atmosphere using co2 emitter, traditional map, and vacuum. | pieces of prerigor salmon fillets were packaged in modified atmosphere (60% co(2) and 40% n(2)) and in vacuum. the ma packages had a gas to product volume ratio (g/p ratio) of 3/1 (traditional map) and 1/1 (packaged with a co(2) emitter). all the samples were stored at 1.2 degrees c for 25 d. the ma packages had lower bacterial growth during storage compared to vacuum packages. the analyses of 16s rrna at day 22 indicated a similar bacterial diversity, independent of packaging methods, dominated ... | 2009 | 19723208 |
| remarkable resistance to uvb of the marine bacterium photobacterium angustum explained by an unexpected role of photolyase. | dna damage and cell survival was assessed in the marine bacteria, photobacterium angustum (gc%=39.6) and sphingopyxis alaskensis (gc%=65.5) following uvb irradiation and recovery in the presence or absence of visible light. the extent of bipyrimidine photoproduct formation was analyzed by hplc-ms/ms. s. alaskensis was chosen as a reference species since it was previously shown to be photoresistant. interestingly, p. angustum exhibited an even higher level of survival to uvb irradiation than s. a ... | 2009 | 19707619 |
| importance of proteins controlling initiation of dna replication in the growth of the high-pressure-loving bacterium photobacterium profundum ss9. | the molecular mechanism(s) by which deep-sea bacteria grow optimally under high hydrostatic pressure at low temperatures is poorly understood. to gain further insight into the mechanism(s), a previous study screened transposon mutant libraries of the deep-sea bacterium photobacterium profundum ss9 and identified mutants which exhibited alterations in growth at high pressure relative to that of the parent strain. two of these mutants, fl23 (pbpra3229::mini-tn10) and fl28 (pbpra1039::mini-tn10), w ... | 2009 | 19700526 |
| [toxicity of nitrification substrates and products to photobacterium]. | in this study, we studied the toxic effect of nitrification substrates and products on photobacterium. | 2009 | 19673411 |
| innovations in host and microbial sialic acid biosynthesis revealed by phylogenomic prediction of nonulosonic acid structure. | sialic acids (sias) are nonulosonic acid (nulo) sugars prominently displayed on vertebrate cells and occasionally mimicked by bacterial pathogens using homologous biosynthetic pathways. it has been suggested that sias were an animal innovation and later emerged in pathogens by convergent evolution or horizontal gene transfer. to better illuminate the evolutionary processes underlying the phenomenon of sia molecular mimicry, we performed phylogenomic analyses of biosynthetic pathways for sias and ... | 2009 | 19666579 |
| comparative study of immobilized and soluble nadh:fmn-oxidoreductase-luciferase coupled enzyme system. | the properties of a coupled enzyme system (nad(p)h:fmn-oxidoreductase and luciferase) from luminous bacteria were studied. the enzymes and their substrates were immobilized in polymer gels of different types: starch (polysaccharide) and gelatin (polypeptide). maximum activity yield (100%) was achieved with the enzymes immobilized in starch gel. an increase in k(m) (app) was observed in both immobilized systems as compared with the soluble coupled enzyme system. immobilization in starch and gelat ... | 2009 | 19645676 |
| differentiation of whole bacterial cells based on high-throughput microarray chip printing and infrared microspectroscopic readout. | using robotic automation, a microarray printing protocol for whole bacterial cells was developed for subsequent label-free and nondestructive infrared microspectroscopic detection. using this contact microspotting system, 24 microorganisms were printed on zinc selenide slides; these were 6 species of listeria, 10 species of vibrio, 2 strains of photobacterium damselae, yersinia enterocolitica 289, bacillus cereus atcc 14529, staphylococcus aureus, atcc 19075 (serotype 104 b), shigella sonnei 201 ... | 2009 | 19630511 |
| a concise diels-alder strategy for the asymmetric synthesis of (+)-albicanol, (+)-albicanyl acetate, (+)-dihydrodrimenin, and (-)-dihydroisodrimeninol. | a short, mild, highly diastereo-, regio-, and stereoselective diels-alder strategy has been developed for the asymmetric synthesis of (+)-albicanol, (+)-albicanyl acetate, (+)-dihydrodrimenin, and (-)-dihydroisodrimeninol. | 2009 | 19594165 |
| a novel packaging method with a dissolving co(2) headspace combined with organic acids prolongs the shelf life of fresh salmon. | the aim of this study was to evaluate a novel packaging method for fresh fish and to determine its effect on the bacterial growth in fresh salmon. fresh salmon was packed with a small amount of 100% co(2) (gas/product ratio 0.2/1.0 v/v) and a brine solution containing various combinations of citric acid (3% w/w, ph 5), acetic acid (1% w/w, ph 5) and cinnamaldehyde (200 microg/ml). total bacterial counts, counts of sulphur reducing bacteria, lactic acid bacteria and enterobacteriaceae as well as ... | 2009 | 19523706 |
| photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater. | two luminous marine bacteria, strains lc2-065(t) and lc2-102, were isolated from seawater at sagami bay in japan. these bacteria were gram-negative, oxidase-negative, catalase-positive, motile and coccoid-rods. 16s rrna gene sequence analysis and multilocus sequence analysis (mlsa) using six loci (ftsz, gapa, gyrb, mreb, pyrh and topa) and sequence analysis of the alpha subunit of luciferase (luxa) gene revealed that these bacteria were distinct from other species of the genus photobacterium. th ... | 2009 | 19502330 |
| development of a multiplex pcr assay for photobacterium damselae subsp. piscicida identification in fish samples. | a multiplex polymerase chain reaction protocol for the detection of photobacterium damselae and subspecies piscicida and damselae discrimination, with internal amplification control, was developed. assay specificity was assessed by testing 19 target and 25 non-target pure cultures. the detection limit was 500 fg, corresponding to 100 genome equivalents. the optimized protocol was also prevalidated with spleen, kidney and blood samples from infected and uninfected sea bass, without any culture st ... | 2009 | 19500208 |
| expression and characterization of the periplasmic cobalamin-binding protein of photobacterium damselae subsp. piscicida. | abstract cobalamin (vitamin b(12)) is an essential cofactor in a variety of enzymatic reactions and most prokaryotes contain transport systems to import vitamin b(12). a gene coding for a periplasmic cobalamin-binding protein of photobacterium damselae subsp. piscicida was identified by in silico analysis of sequences from a genomic library. the open reading frame was composed of 834 bp encoding a protein of 277 amino acids. the protein showed 61% identity with the vitamin b(12)-binding protein ... | 2009 | 19490395 |
| synthesis of new 2-thio-[1,2,4]triazolo[1,5-c]quinazoline derivatives and its antimicrobial activity. | a series of novel ([1,2,4]triazolo[1,5-c]quinazolin-2-ylthio)carboxylic acids 2a-d and esters 3a-l were synthesized and evaluated for antimicrobial activity. alkylation of potassium 2-thio-[1,2,4]triazolo[1,5-c]quinazoline 1 with halogenocarboxylic acids and its esters proceeded s-regioselectively. during acid catalyzed esterification of 2a-c, degradation of the pyrimidine ring was observed. the structures of the compounds were elucidated by ft-ir, (1)h- and (13)c-nmr, electron impact mass spect ... | 2009 | 19483337 |
| multi-gene analysis reveals previously unrecognized phylogenetic diversity in aliivibrio. | the "vibrio fischeri species group" recently was reclassified as a new genus, aliivibrio, comprising four species, aliivibrio fischeri, aliivibrio logei, aliivibrio salmonicida, and aliivibrio wodanis. only limited phylogenetic analysis of strains within aliivibrio has been carried out, however, and taxonomic ambiguity is evident within this group, especially for phenotypically unusual strains and certain strains isolated from bioluminescent symbioses. therefore, to examine in depth the evolutio ... | 2009 | 19481895 |
| crystal structure of alpha/beta-galactoside alpha2,3-sialyltransferase from a luminous marine bacterium, photobacterium phosphoreum. | alpha/beta-galactoside alpha2,3-sialyltransferase produced by photobacterium phosphoreum jt-ish-467 is a unique enzyme that catalyzes the transfer of n-acetylneuraminic acid residue from cytidine monophosphate n-acetylneuraminic acid to acceptor carbohydrate groups. the enzyme recognizes both mono- and di-saccharides as acceptor substrates, and can transfer neu5ac to both alpha-galactoside and beta-galactoside, efficiently. to elucidate the structural basis for the broad acceptor substrate speci ... | 2009 | 19467231 |
| invasion and survival of photobacterium damselae subsp. piscicida in non-phagocytic cells of gilthead sea bream, sparus aurata l. | fluorescence microscopy and gentamicin protection assays were used to investigate the ability of four photobacterium damselae subsp. pisicida (phdp) strains to adhere to and to invade the fish epithelial cell line, saf-1, derived from sparus aurata. all strains tested were detected intracellularly using both techniques, although internalization levels varied among strains. treatment with cytochalasin d and experiments carried out at 4 degrees c demonstrated that a functional host cell cytoskelet ... | 2009 | 19460085 |
| catalytic properties of a lipase from photobacterium lipolyticum for biodiesel production containing a high methanol concentration. | biodiesel, an alternative fuel, is generated via the transesterification reaction of vegetable oil or animal oil with alcohol. currently, many reports have noted that microbial lipases might be utilized for the production of biodiesel. among them, immobilized candida antarctica lipase b (novozym435) is frequently utilized for its biocatalytic efficiency and availability. however, as the enzyme is unstable in a medium containing high concentrations of methanol, a multi-stepwise methanol supply is ... | 2009 | 19447334 |
| transferrin and ferritin response to bacterial infection: the role of the liver and brain in fish. | iron is essential for growth and survival, but it is also toxic when in excess. thus, there is a tight regulation of iron that is accomplished by the interaction of several genes including the iron transporter transferrin and iron storage protein ferritin. these genes are also known to be involved in response to infection. the aim of this study was to understand the role of transferrin and ferritin in infection and iron metabolism in fish. thus, sea bass transferrin and ferritin h cdnas were iso ... | 2009 | 19428486 |
| expression, purification, and characterization of the recombinant putative periplasmic hemin-binding protein (hutb) of photobacterium damselae subsp. piscicida. | hutb, the periplasmic hemin binding protein of photobacterium damselae subsp. piscicida, was produced as a recombinant protein. uv-vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. spectrophotometric titration of hutb with hemin showed saturation at a heme/hutb ratio of 1:1 and a binding affinity (k(d)) of 10 microm. | 2009 | 19420687 |
| characterization of dissolved organic matter fractions and its relationship with the disinfection by-product formation. | dissolved organic matter (dom) has been identified as precursor for disinfection by-products (dbps) formation during chlorination. recently, it has been demonstrated that the characteristics of dom influence the dbps formation mechanism. a study was, therefore, initiated to investigate the effects of dom fractions on dbps formation mechanism. in the chlorination process, organic acids are dominant precursors of total thihalomethanes (tthm) because of the v(c-o) and unsaturated structures. furthe ... | 2009 | 19402400 |
| utilization of mucus from the coral acropora palmata by the pathogen serratia marcescens and by environmental and coral commensal bacteria. | in recent years, diseases of corals caused by opportunistic pathogens have become widespread. how opportunistic pathogens establish on coral surfaces, interact with native microbiota, and cause disease is not yet clear. this study compared the utilization of coral mucus by coral-associated commensal bacteria ("photobacterium mandapamensis" and halomonas meridiana) and by opportunistic serratia marcescens pathogens. s. marcescens pdl100 (a pathogen associated with white pox disease of acroporid c ... | 2009 | 19395569 |
| photobacterium damselae subsp. damselae, an emerging pathogen in danish rainbow trout, oncorhynchus mykiss (walbaum), mariculture. | a selection of 16 field isolates of photobacterium damselae from marine rainbow trout farms in denmark was subjected to phenotypic and genotypic characterization and pathogenicity to fish. all isolates belonged to the subspecies damselae, being positive for haemolysis, motility and urease. there were considerable differences in haemolytic properties, some isolates presenting a broad zone of haemolysis and others only a narrow zone. pulsed-field gel electrophoresis revealed a high diversity indic ... | 2009 | 19364386 |
| vibrios as causal agents of zoonoses. | vibrios are gram-negative rod-shaped bacteria that are widespread in the coastal and estuarine environments. some species, e.g. vibrio anguillarum and v. tapetis, comprise serious pathogens of aquatic vertebrates or invertebrates. other groups, including grimontia (=vibrio) hollisae, photobacterium (=vibrio) damselae subsp. damselae, v. alginolyticus, v. harveyi (=v. carchariae), v. cholerae, v. fluvialis, v. furnissii, v. metschnikovii, v. mimicus, v. parahaemolyticus and v. vulnificus, may cau ... | 2010 | 19342185 |
| the nonrandom microheterogeneity of 16s rrna genes in vibrio splendidus may reflect adaptation to versatile lifestyles. | 16s rrna molecules in a microbial strain can differ due to nucleotide variation between their genes. this is a typical trait of fast-growing bacteria to cope with different niches. we investigated characteristics of 16s rrna genes in vibrio splendidus strain pb1-10, from the normal flora of atlantic halibut. sequencing of 16s rrna gene clones detected 35 variable positions in a total of 13 different gene copies. more than two-thirds of the substitutions occurred in regions corresponding to helix ... | 2009 | 19341395 |
| induction of the histidine decarboxylase genes of photobacterium damselae subsp. damselae (formally p. histaminum) at low ph. | to elucidate the detailed mechanism of histamine production by photobacterium damselae subsp. damselae. | 2009 | 19302297 |
| effect of increasing nitrobenzene loading rates on the performance of anaerobic migrating blanket reactor and sequential anaerobic migrating blanket reactor/completely stirred tank reactor system. | a laboratory scale anaerobic migrating blanket reactor (ambr) reactor was operated at nitrobenzene (nb) loading rates increasing from 3.33 to 66.67 g nb/m(3)day and at a constant hydraulic retention time (hrt) of 6 days to observe the effects of increasing nb concentrations on chemical oxygen demand (cod), nb removal efficiencies, bicarbonate alkalinity, volatile fatty acid (vfa) accumulation and methane gas percentage. moreover, the effect of an aerobic completely stirred tank reactor (cstr) re ... | 2009 | 19286317 |
| photobacterium damsela wound infection in a 14-year-old surfer. | photobacterium damsela (p damsela) is a common marine bacterium. rare reports of p damsela causing septicemia and wound infection have been described in children. we report a case of photobacterium damsela isolated from a wound culture taken from a 14-year-old male injured after a minor trauma while surfing. we alert physicians to this infection and the importance of early diagnosis and appropriate antimicrobial therapy for wound infection following seawater injury. | 2009 | 19279534 |
| piscidin 4, a novel member of the piscidin family of antimicrobial peptides. | piscidins are linear, amphipathic, antimicrobial peptides (amps) with broad, potent, activity spectrum. piscidins and other members of the piscidin family appear to comprise the most common group of amps in teleost fish. all piscidins and related members of the piscidin family described to date are 18-26 amino acids long.we report here the isolation of a novel 5329.25 da, 44-residue (ffrhlfrgakaifrgarqgxrahkvvsryrnrdvpetdnnqeep) antimicrobial peptide from hybrid striped bass (morone chrysops fem ... | 2009 | 19266617 |
| evaluation of the ecotoxicity of model nanoparticles. | since society at large became aware of the use of nanomaterials in ever growing quantities in consumer products and their presence in the environment, critical interest in the impact of this emerging technology has grown. the main concern is whether the unknown risks of engineered nanoparticles (nps), in particular their impact on health and environment, outweighs their established benefits for society. therefore, a key issue in this field is to evaluate their potential toxicity. in this context ... | 2009 | 19264345 |
| detection of bacterioplankton in immersed cadavers using selective agar plates. | we measured bacterioplankton in blood from cadavers retrieved from the sea (n=12), near estuaries (n=4), rivers (fresh water, n=8) and from bathtubs (n=4) as well as from non-drowned victims (n=10) discovered near aquatic environments. blood from 11 victims drowned in seawater developed bioluminescent and/or blue colonies (oxidase test positive) on selective media containing 2-4% nacl. homology analyses of the 16s rrna gene showed that all of them were marine bacteria (genera: photobacterium, vi ... | 2009 | 19261520 |
| combined photobacterium toxicity of herbicide mixtures containing one insecticide. | to test whether the dose-addition (da) model can predict the combined toxicity of the mixtures of herbicides that coexisted with insecticide(s), we selected five herbicides (simetryn, prometon, bromacil, velpar, and diquat) and one organophosphorus insecticide (dichlorvos) as the test components. the inhibition toxicities of the six pesticides as well as those of their mixtures to vibrio qinghaiensis sp.-q67 were determined by using the microplate toxicity test procedure. the dose-response curve ... | 2009 | 19215957 |
| bioluminescence intensity difference observed in luminous bacteria groups with different motility. | the aim of this study was to compare the luminescent intensity of bioluminescence from marine luminous bacteria with different motility. | 2009 | 19207857 |
| comparison between the short-term and the long-term toxicity of six triazine herbicides on photobacteria q67. | the bioluminescence inhibition of six triazine herbicides including desmetryne (des), simetryn (sim), velpar (vel), prometon (pro), metribuzin (met), and aminotriazine (ami) on vibrio qinghaiensis sp.-q67 (q67) was determined to investigate the effects of exposure duration on the ecotoxicological relevance of triazine herbicides. based on the short-term microplate toxicity analysis (mta), a long-term mta was established to assess the impact of exposure time on the toxicities of the herbicides. t ... | 2009 | 19203776 |
| [construction of recombinate luminescence bacteria vector to evaluate the genetoxic of environment pollutant]. | recombinate luminescence bacteria have the important role in evaluating water toxicity. two recombinate luminescence bacteria vectors pucd-uvra and pucd-alka were constructed to investigate the impaired mechanism of pollutant genetic toxicity. the genes of uvra and alka were amplified by pcr from e. coli w3110, sequenced after ligated with pgem-t easy vector. the pcr products and pucd615 vector were all digested with bamh i, ecor i, then be linked and imported into jm109 with electrotransformati ... | 2008 | 19186821 |
| effect of quinone on the fluorescence decay dynamics of endogenous flavin bound to bacterial luciferase. | the interaction of quinone with luciferase from photobacterium leiognathi was studied based on the fluorescence decay measurements of the endogenous flavin bound to the enzyme. homologous 1,4-quinones, 1,4-benzoquinone, methyl-1,4-benzoquinone, 2-methyl-5-isopropyl-1,4-benzoquine and 1,4-naphthoquinone, were investigated. in the absence of quinone, the fluorescence intensity and anisotropy decays of the endogenous flavin exhibited two intensity decay lifetimes (~1 and 5 ns) and two anisotropy de ... | 2009 | 19162392 |
| [adaptation of bioluminescence technique for the evaluation of the toxicity of mineral waters]. | mineral waters have been ascertained to exert a pronounced inhibiting effect, that is in proportion in their mineralization and ph values, on the fluorescence of microbial luminescent biosensors. to prevent such adverse reactions masking the effects of chemical pollutants, the authors propose an adjusted procedure for preparing the samples of mineral waters for luminescence biotesting. the first approach is to differentially insert additional quantities of nacl into the test samples depending on ... | 2008 | 19097443 |
| observations on the estrogenic activity and concentration of 17beta-estradiol in the discharges of 12 wastewater treatment plants in southern australia. | there is very little information on the overall level of estrogenic activity, or concentrations of specific hormonal compounds in wastewater treatment plant (wwtp) discharges in australia, compared with europe, japan, and north america. to partly address this, in 2004, water samples were collected as "grab" or "spot" samples from 12 wwtp facilities across southern victoria at the point at which effluent enters the environment, either as recycled water or direct discharge to the receiving water. ... | 2009 | 19057835 |
| protein evolution in deep sea bacteria: an analysis of amino acids substitution rates. | abyssal microorganisms have evolved particular features that enable them to grow in their extreme habitat. genes belonging to specific functional categories are known to be particularly susceptible to high-pressure; therefore, they should show some evidence of positive selection. to verify this hypothesis we computed the amino acid substitution rates between two deep-sea microorganisms, photobacterium profundum ss9 and shewanella benthica kt99, and their respective shallow water relatives. | 2008 | 19014525 |
| 15n metabolic test for the determination of phytotoxic effects of chemicals and contaminated environmental samples. | a stable isotope(15)n-nitrogen test (esima = ecotoxicological stable isotope metabolic assay) was developed to assess biological effects and the potential toxicological hazard of chemicals and contaminated environmental samples on plant metabolism. the assay measures the effect of toxicants on the incorporation of a(15)n labelled tracer into the total nitrogen fraction (both the nonprotein and protein fraction) of plants. segments of pisum arvense epicotyls are used as test substrates because of ... | 1999 | 19009406 |
| developmental and microbiological analysis of the inception of bioluminescent symbiosis in the marine fish nuchequula nuchalis (perciformes: leiognathidae). | many marine fish harbor luminous bacteria as bioluminescent symbionts. despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of their symbioses. to gain insight on these issues, we examined wild-caught larvae of the leiognathid fish nuchequula nuchalis, a species that harbors photobacterium leiognathi as its ... | 2008 | 18978090 |
| biodegradability and toxicity of sulphonate-based surfactants in aerobic and anaerobic aquatic environments. | four types of commonly used sulphonate-based surfactants (alkane sulphonates, alpha-olefin sulphonates, sulphosuccinates and methyl ester sulphonates) were tested for their aerobic and anaerobic biodegradability as well as for their toxicity to daphnia magna and photobacterium phosphoreum to assess the effect of the surfactant structure on those properties. aerobic biodegradation was evaluated by means of the co2 headspace test and anaerobic biodegradation was assessed by a method based on the e ... | 2009 | 18976786 |
| [quantitative criteria for the estimation of the effectiveness of bioluminescence expression in natural and transgenic luminescent bacteria]. | computation coefficients for estimating the effectiveness of bioluminescence expression in natural luminescent bacteria p. leiognathi 54 and transgenic strain e. coli z905/pphl7 bearing lux-operon in multicopy plasmid are suggested, and their use on the molecular, cell, and population levels was considered. it was shown that, on the population level, all transgenic variants got the better of natural variants of p. leiognathi 54 irrespective of the type of lux-operon regulation. on the cell level ... | 2008 | 18954013 |
| [biotests for mineral waters with natural and recombinant luminescent microorganisms]. | we have developed methods of biotesting mineral waters involving use of natural or recombinant luminescent strains with elimination of the effect of salt concentration and ph. to overcome the adverse effect of high salt concentrations, disguising the action of chemical pollutants, a special method of mineral water sample preparation is proposed. in this method, the marine luminescent bacterium photobacterium phosphoreum (microbiosensor b17 677f) is used as a test object. samples to be analyzed a ... | 2008 | 18924408 |
| photocatalytic disinfection of marine bacteria using fluorescent light. | photocatalytic oxidation (pco) using fluorescent light was used to disinfect two marine bacteria: alteromonas alvinellae and photobacterium phosphoreum. results showed that p. phosphoreum exhibited a lower susceptibility towards pco than a. alvinellae, which was related to their fatty acid profiles and levels of superoxide dismutase (sod) and catalase (cat). results also revealed that cat and sod levels would be triggered by pco reaction. in addition, pco disinfection efficiency could be enhance ... | 2008 | 18842281 |
| [toxicity screening and identification of bacteria isolated from snails nassarius semiplicatus and their habitat]. | tetrodotoxin and its analogues (ttxs) were responsible for the poisoning incidents associated with snail nassarius spp. we studied bacteria isolated from toxic snails as well as their habitat to probe into the relationship between bacteria and toxicity of nassariid gastropod. | 2008 | 18837369 |
| study on the synergic effect of natural compounds on the microbial quality decay of packed fish hamburger. | the effectiveness of natural compounds in slowing down the microbial quality decay of refrigerated fish hamburger is addressed in this study. in particular, the control of the microbiological spoilage by combined use of three antimicrobials, and the determination of their optimal composition to extend the fish hamburger microbiological stability limit (mal) are the main objectives of this work. thymol, grapefruit seed extract (gfse) and lemon extract were tested for monitoring the cell growth of ... | 2008 | 18804302 |
| c-lysozyme from senegalese sole (solea senegalensis): cdna cloning and expression pattern. | lysozymes are key molecules of innate immunity and proved high bactericidal activity in fish, thus becoming attractive as tools for enhancing fish defences. in this study, a full-length c-type lysozyme cdna from senegalese sole (solea senegalensis) has been cloned and characterized. the cdna sequence was inferred from two overlapping fragments obtained by race-pcr and consisting on 631bp coding for 143 aminoacids. catalytic and other conserved residues required for lysozyme activity were identif ... | 2008 | 18786641 |
| surveillance of health status on eight marine rainbow trout, oncorhynchus mykiss (walbaum), farms in denmark in 2006. | the health status of eight marine rainbow trout farms was followed from mid-june to mid-september 2006 by sampling both dead and healthy fish approximately every 2 weeks for bacteriological and virological investigation. no fish pathogenic viruses were detected, but all farms experienced disease and mortality as a result of various bacterial infections. yersinia ruckeri was found on four and renibacterium salmoninarum on five of the farms, but only during the first part of the surveillance perio ... | 2008 | 18786028 |
| gammaproteobacteria as a possible source of eicosapentaenoic acid in anoxic intertidal sediments. | eicosapentaenoic acid (epa; n-20:5omega3) was found to be a constituent of phospholipids in three mesophilic strains of gammaproteobacteria, which were isolated from anoxic most probable number series prepared with sediments from an intertidal flat of the german north sea coast. their partial 16s rrna gene sequences identified the isolates as close relatives of shewanella colwelliana, vibrio splendidus, and photobacterium lipolyticum. so far, eicosapentaenoic acid has mainly been reported to occ ... | 2009 | 18777187 |
| the deep-sea bacterium photobacterium profundum ss9 utilizes separate flagellar systems for swimming and swarming under high-pressure conditions. | motility is a critical function needed for nutrient acquisition, biofilm formation, and the avoidance of harmful chemicals and predators. flagellar motility is one of the most pressure-sensitive cellular processes in mesophilic bacteria; therefore, it is ecologically relevant to determine how deep-sea microbes have adapted their motility systems for functionality at depth. in this study, the motility of the deep-sea piezophilic bacterium photobacterium profundum ss9 was investigated and compared ... | 2008 | 18723648 |
| [synthesis of reserve polyhydroxyalkanoates by luminescent bacteria]. | the ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, pha) as reserve macromolecules was studied. twenty strains from the collection of the luminescent bacteria ccibso (wdsm839) of the institute of biophysics, siberian branch, russian academy of sciences, assigned to different taxa (photobacterium leiognathi, ph. phosphoreum, vibrio harveyi, and v. fischeri) were analyzed. the most productive strains were identified, and the condit ... | 2008 | 18683654 |
| location of lectin exhibiting specificity for n-acetyl-d-galactosamine in cells of the symbiotic marine bacteria photobacterium phosphoreum. | 2008 | 18680915 | |
| [effect of salts on luminescence of natural and recombinant luminescent bacterial biosensors]. | effect of cations k+, na+, mg2+, and ca2+ and anions so4(2-), hco3(-), and co3(2-) on the luminescence intensity of the marine luminescent bacterium photobacterium phorphoreum (microbiosensor b-17 677f) and the recombinant strain escherichia coli with cloned lux operon of p. leiognathi (ekolyum-9). it is found that small concentrations of chlorides and sulfates of the cations studied had a concentration-dependent stimulatory effect on bacterial bioluminescence; as the concentration of agents inc ... | 2008 | 18663957 |
| comparative study of phenol and cyanide containing wastewater in cstr and sbr activated sludge reactors. | the objectives of this work were the examination of the performance of two bench scale activated sludge systems, a conventional continuous stirring tank reactor (cstr) and a sequential batch reactor (sbr), for the treatment of wastewaters containing phenol and cyanides and the assessment of the toxicity reduction potential by bioassays. the operation of the reactors was monitored by physicochemical analyses, while detoxification potential of the systems was monitored by two bioassays, the marine ... | 2009 | 18650084 |