Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| edwardsiella tarda bacteremia with metastatic gastric cancer. | edwardsiella tarda (e. tarda) is a rare human pathogen; however, the overall mortality of bacteremia is reported to be up to 50%. here, we describe a case of cholangitis with e. tarda bacteremia who had a pancreatoduodenectomy for a locally advanced gastric cancer. he was successfully treated using a cefmetazole, a second generation cephalosporin for 14 days. to the best of our knowledge, this is the first case report about e. tarda bacteremia after biliary reconstruction. | 2016 | 27516970 |
| eha, a regulator of edwardsiella tarda, required for resistance to oxidative stress in macrophages. | edwardsiella tarda is distributed widely in a variety of hosts. eha has recently been found to be its virulence regulator. in order to explore the mechanism of its regulation, we investigated the survival rates of wild type strain et13, and its eha mutant and complemented strains in raw264.7 macrophages under light microscopic observation as well as by counting bacterial cfus on the plates. all of the different strains could live within the macrophages; however, the intracellular numbers of the ... | 2016 | 27511959 |
| edwardsiella tarda-induced inhibition of apoptosis: a strategy for intracellular survival. | edwardsiella tarda is a gram-negative bacterial pathogen that can infect a wide range of freshwater and marine fish. one salient feature of e. tarda is the ability to survive and replicate in various host cells. in this study, we observed that e. tarda replicated robustly in the zebrafish cell line zf4, and that e. tarda-infected cells exhibited no detectable signs of apoptosis. global transcriptome analysis and quantitative real-time rt-pcr revealed that e. tarda infection generally significant ... | 2016 | 27471679 |
| identification of the interactome between fish plasma proteins and edwardsiella tarda reveals tissue-specific strategies against bacterial infection. | elucidating the complex pathogen-host interaction is essential for a comprehensive understanding of how these remarkable agents invade their hosts and how the hosts defend against these invaders. during the infection, pathogens interact intensively with host to enable their survival, which can be revealed through their interactome. edwardsiella tarda is a gram-negative bacterial pathogen causing huge economic loss in aquaculture and a spectrum of intestinal and extraintestinal diseases in humans ... | 2016 | 27458055 |
| near-drowning-associated pneumonia with bacteremia caused by coinfection with methicillin-susceptible staphylococcus aureus and edwardsiella tarda in a healthy white man: a case report. | edwardsiella tarda is an enterobacteriaceae found in aquatic environments. extraintestinal infections caused by edwardsiella tarda in humans are rare and occur in the presence of some risk factors. as far as we know, this is the first case of near-drowning-associated pneumonia with bacteremia caused by coinfection with methicillin-susceptible staphylococcus aureus and edwardsiella tarda in a healthy patient. | 2016 | 27423181 |
| edwardsiella tarda outer membrane protein c: an immunogenic protein induces highly protective effects in flounder (paralichthys olivaceus) against edwardsiellosis. | outer membrane protein c of edwardsiella tarda is a major cell surface antigen and it was identified to be an immunogenic protein by western blot using flounder (paralichthys olivaceus) anti-recombinant ompc (rompc), and anti-e. tarda antibodies. rompc tested the immune protective effect against e. tarda challenge in a flounder model and produced a relative percentage of survival rate of 85%. the immune response of flounder induced by rompc was investigated, and the results showed that: (1) the ... | 2016 | 27420049 |
| three-dimensional visualization of green fluorescence protein-labelled edwardsiella tarda in whole medaka larvae. | the invasive fish pathogen edwardsiella tarda is common in aquatic environments and causes the environmentally and economically destructive emphysematous putrefactive disease called edwardsiellosis. in order to understand the organism's infection pathway, medaka larvae (oryzias latipes) were immersion-infected with e. tarda labelled with green fluorescence protein (gfp) and then visualized in three dimensions under confocal laser microscopy and light-sheet fluorescence microscopy. confocal micro ... | 2017 | 27416977 |
| tolc plays a crucial role in immune protection conferred by edwardsiella tarda whole-cell vaccines. | although vaccines developed from live organisms have better efficacy than those developed from dead organisms, the mechanisms underlying this differential efficacy remain unexplored. in this study, we combined sub-immunoproteomics with immune challenge to investigate the action of the outer membrane proteome in the immune protection conferred by four edwardsiella tarda whole-cell vaccines prepared via different treatments and to identify protective immunogens that play a key role in this immune ... | 2016 | 27406266 |
| antimicrobial activity of essential oil of eucalyptus globulus against fish pathogenic bacteria. | the antibacterial activities of the essential oil of eucalyptus globulus (eoeg) was determined against 7 fish pathogenic bacteria (edwardsiella tarda, streptococcus iniae, s. parauberis, lactococcus garviae, vibrio harveyi, v. ichthyoenteri and photobacterium damselae) obtained from farmed olive flounder. the inhibitory activity was evaluated by three methods: disc diffusion method, minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc). according to the disc diffusi ... | 2016 | 27382376 |
| molecular cloning and expression analysis of a new lily-type lectin in the rock bream, oplegnathus fasciatus. | a new lily-type lectin rbltl was identified from rock bream (oplegnathus fasciatus) and its expression analysed. in this study, a new lily-type lectin gene (rbltl) was cloned from rock bream using expressed sequence tag (est) analysis. the full-length rbltl cdna was encoding a 117-amino acid protein. the deduced amino acid sequence of rbltl contained all of the conserved features crucial for its fundamental structure, including b-lectin domain and three d-mannose binding sites. rbltl mrna was pr ... | 2016 | 27345170 |
| distinct immune tones are established by lactococcus lactis bfe920 and lactobacillus plantarum fgl0001 in the gut of olive flounder (paralichthys olivaceus). | the immune tone is defined as an immunological state during which the readiness for immune response is potentiated. the establishment of immune tone in the gut of olive flounder (paralichthys olivaceus) was investigated by feeding lactococcus lactis bfe920 (ll) or lactobacillus plantarum fgl0001 (lp). ll-fed flounder showed significantly increased levels of regulatory genes (foxp3, il-10, and tgf-β1), cd18, and cd83 in the gut. in contrast, lp feeding drastically increased proinflammatory genes ... | 2016 | 27320869 |
| expression and role of gap junction protein connexin43 in immune challenge-induced extracellular atp release in japanese flounder (paralichthys olivaceus). | connexin43 (cx43) is the best characterized gap junction protein that allows the direct exchange of signaling molecules during cell-to-cell communications. the immunological functions and atp permeable properties of cx43 have been insensitively examined in mammals. the similar biological significance of cx43 in lower vertebrates, however, is not yet understood. in the present study we identified and characterized a cx43 ortholog (termed pocx43) from japanese flounder (paralichthys olivaceus) and ... | 2016 | 27291350 |
| computer-aided vaccine designing approach against fish pathogens edwardsiella tarda and flavobacterium columnare using bioinformatics softwares. | edwardsiella tarda and flavobacterium columnare are two important intracellular pathogenic bacteria that cause the infectious diseases edwardsiellosis and columnaris in wild and cultured fish. prediction of major histocompatibility complex (mhc) binding is an important issue in t-cell epitope prediction. in a healthy immune system, the t-cells must recognize epitopes and induce the immune response. in this study, t-cell epitopes were predicted by using in silico immunoinformatics approach with t ... | 2016 | 27284239 |
| edwardsiella tarda esae (orf19 protein) is required for the secretion of type iii substrates, and pathogenesis in fish. | type iii secretion system (t3ss) is a large macromolecular assembly found on the surface of many pathogenic gram-negative bacteria. edwardsiella tarda is an important gram-negative pathogen that employs t3ss to deliver effectors into host cells to facilitate its survival and replication. eseb, esec, and esed, when secreted, form a translocon complex esebcd on host membranes through which effectors are translocated. the orf19 gene (esae) of e. tarda is located upstream of esak, and downstream of ... | 2016 | 27283851 |
| esee of edwardsiella tarda augments secretion of translocon protein esec and expression of the escc-esee operon. | edwardsiella tarda is an important gram-negative pathogen that employs a type iii secretion system (t3ss) to deliver effectors into host cells to facilitate bacterial survival and replication. these effectors are translocated into host cells through a translocon complex composed of three secreted proteins, namely, eseb, esec, and esed. the secretion of eseb and esed requires a chaperone protein called escc, whereas the secretion of esec requires the chaperone esca. in this study, we identified a ... | 2016 | 27271743 |
| antigen uptake and expression of antigen presentation-related immune genes in flounder (paralichthys olivaceus) after vaccination with an inactivated edwardsiella tarda immersion vaccine, following hyperosmotic treatment. | antigen uptake is a critical process for activation of the immune system, and therefore the ability to enhance antigen uptake is a primary consideration in the development of an immersion vaccination of fish. in the present work, flounders (paralichthys olivaceus) were immersed in three hyperosmotic solutions with 40, 50 and 60‰ salinities, then transferred into seawater of normal salinity (i.e. 30‰) containing formalin-inactivated edwardsiella tarda for 30 min. the antigen uptake in vaccinated ... | 2016 | 27263114 |
| the serine protease autotransporter tsh contributes to the virulence of edwardsiella tarda. | the temperature-sensitive hemagglutinin (tsh), identified as serine protease autotransporters of the enterobacteriaceae (spates) proteins, is an important virulence factor for avian-pathogenic escherichia coli (apec) and uropathogenic e. coli. however, little is known about the role of tsh as a virulence factor in edwardsiella tarda, a severe fish pathogen. in this study, we characterized the tsh of e. tarda (named tshet) and examined its function and vaccine potential. tshet is composed of 1224 ... | 2016 | 27259829 |
| lactoferrin decreases the intestinal inflammation triggered by a soybean meal-based diet in zebrafish. | intestinal inflammation is a harmful condition in fish that can be triggered by the ingestion of soybean meal. due to the positive costs-benefits ratio of including soybean meal in farmed fish diets, identifying additives with intestinal anti-inflammatory effects could contribute to solving the issues caused by this plant protein. this study evaluated the effect of incorporating lactoferrin (lf) into a soybean meal-based diet on intestinal inflammation in zebrafish. larvae were fed with diets co ... | 2016 | 27247950 |
| nod2 in zebrafish functions in antibacterial and also antiviral responses via nf-κb, and also mda5, rig-i and mavs. | nod2/ripk2 signalling plays essential role in the modulation of innate and adaptive immunity in mammals. in this study, nod2 was functionally characterized in zebrafish (danio rerio), and its interaction with a receptor-interaction protein, ripk2, and rlrs such as mda5 and rig-i, as well as the adaptor, mavs was revealed in fish innate immunity. the expression of nod2 and ripk2 in zf4 cells has been constitutive and can be induced by the infection of edwardsiella tarda and svcv. the nod2 can sen ... | 2016 | 27235368 |
| intracellular translocation and localization of edwardsiella tarda type iii secretion system effector eseg in host cells. | edwardsiella tarda, an important fish pathogenic bacterium, could utilize type iii secretion system (t3ss) to transfer multiple effector proteins into host cells during infection. eseg was identified to be an e. tarda t3ss effector, which could be injected by t3ss into non-phagocytic cells. since e. tarda is a facultative intracellular pathogen that resides and replicates in macrophage, it is interesting to expand our knowledge about eseg translocation and localization within phagocytic cells. h ... | 2016 | 27208750 |
| a prebiotic role of ecklonia cava improves the mortality of edwardsiella tarda-infected zebrafish models via regulating the growth of lactic acid bacteria and pathogen bacteria. | in this study, the beneficial prebiotic roles of ecklonia cava (e. cava, ec) were evaluated on the growth of lactic acid bacteria (lab) and pathogen bacteria and the mortality of pathogen-bacteria infected zebrafish model. the result showed that the original e. cava (ec) led to the highest growth effects on three labs (lactobacillus brevis, l. brevis; lactobacillus pentosus, l. pentosus; lactobacillus plantarum; l. plantarum) and it was dose-dependent manners. also, ec, its celluclast enzymatic ... | 2016 | 27192145 |
| expression, secretion and bactericidal activity of type vi secretion system in vibrio anguillarum. | the type vi secretion system (t6ss) was recently shown to modulate quorum sensing and the stress response in vibrio anguillarum serotype o1 strain nb10. it is not known whether there is a functionally active t6ss in other serotypes of v. anguillarum. here, homologues to t6ss cluster vtsefgh and hemolysin-coregulated protein (hcp)-encoding genes were found to be prevalent and conserved in clinical isolates of v. anguillarum from fish, including four o1 and five non-o1 serotype strains. unexpected ... | 2016 | 27172981 |
| orf13 in the type iii secretion system gene cluster of edwardsiella tarda binds to the mammalian factor cugbp2. | the type iii secretion system (ttss) is essential for the intracellular replication of edwardsiella tarda in phagocytes of fish and mammals, and a hypothetical gene (orf13) located in the ttss gene cluster is required for intracellular replication and virulence of e. tarda. here, we show that under ttss-inducing conditions, the protein orf13 was secreted into culture supernatant. then, using a yeast 2-hybrid screen, we show that the mammalian factor cugbp2, which regulates apoptosis in breast ca ... | 2016 | 27137075 |
| immunomodulation of lactobacillus pentosus pl11 against edwardsiella tarda infection in the head kidney cells of the japanese eel (anguilla japonica). | wild and farm-raised fish can be simultaneously exposed to different types of pathogens in their habitats. hence, it is important to study their effects, whether isolated or in combination. therefore, the aim of this study was to evaluate the effects of lactobacillus pentosus pl11 on the transcription of specific cytokine genes related to immune response, using japanese eel macrophages as an in vitro model. head kidney leukocytes were isolated from japanese eels and cell viability was determined ... | 2016 | 27108377 |
| cloning and characterization of apoptosis-associated speck-like protein containing a card domain (asc) gene from japanese flounder paralichthys olivaceus. | apoptosis-associated speck-like protein containing a card domain (asc) is a critical adaptor molecule in multiple inflammasome protein complexes that mediate inflammation and host defense. however, few studies have been performed in lower vertebrates such as in teleost. here we identified and characterized a novel asc gene (namely poasc) from japanese flounder paralichthys olivaceus. the complete cdna sequence of poasc contains a 22 bp 5'-untranslated sequence, a 612 bp open reading frame, and a ... | 2016 | 27103005 |
| identification and characterization of atp-gated p2x2 receptor gene dominantly expressed in the japanese flounder (paralichthys olivaceus) head kidney macrophages. | p2x2 receptor (p2x2r) belongs to the family of purinergic receptors that have been shown to play important roles in regulating host innate immune response. although the immunologic significance of p2x2r has been studied in mammals, the presence and immune relevance of p2x2r in fish remains unclear. in this study we extended our previous observations by identifying and characterizing a p2x2r ortholog (termed pop2x2r) from japanese flounder (paralichthys olivaceus). quantitative real-time pcr anal ... | 2016 | 27103003 |
| the global regulatory effect of edwardsiella tarda fur on iron acquisition, stress resistance, and host infection: a proteomics-based interpretation. | ferric uptake regulator (fur) is an important transcriptional regulator of gram-negative bacteria. edwardsiella tarda is a severe fish bacterial pathogen with a broad host range that includes humans. in this study, we examined the regulatory function of fur in e. tarda via a proteomic approach. compared to the wild type tx01, the fur mutant tx01δfur exhibited (i) retarded growth, (ii) enhanced siderophore production, (iii) increased acid tolerance, which is in contrast to observations in other b ... | 2016 | 27102497 |
| identification of genes associated with the penetration activity of the human type of edwardsiella tarda edwgii through human colon epithelial cell monolayers. | edwardsiella tarda is a gram-negative pathogen with a broad host range including fish and humans. e. tarda causes gastrointestinal and extraintestinal infections in humans. in present study, the penetration activities of 22 strains of e. tarda, including 10 human isolates and 12 diseased fish isolates, through caco-2 cell monolayers were evaluated. all the human isolates exhibited penetration activity in contrast to the fish isolates, which did not. in order to identify genes responsible for pen ... | 2016 | 27057670 |
| the role of regulator eha in edwardsiella tarda pathogenesis and virulence gene transcription. | edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. eha is a new transcriptional regulator identified in et13, which is involved in the bacterial hemolytic activity. this study explored the effect of the eha in the pathogenesis of e. tarda and the transcriptional regulation of the bacterial virulence genes (esec, flic, pagc and fima). our results found that the virulence of the eha mutant was 2.5-fold less than the one of its wild et13 by ld50 in a muri ... | 2016 | 27038844 |
| thioredoxin domain-containing protein 12 from oplegnathus fasciatus: molecular characterization, expression against immune stimuli, and biological activities related to oxidative stress. | thioredoxin domain-containing protein 12 (txndc12) is a small, disulfide-containing protein that belongs to the thioredoxin (txn) superfamily. in the present study, we identified and characterized a txndc12-like gene, designated oftxndc12, from rock bream, oplegnathus fasciatus. oftxndc12 consists of seven exons interrupted by six introns. comparative genomic structural analysis revealed that the txndc12 of vertebrates is a structurally conserved gene. the coding sequence of oftxndc12 comprises ... | 2016 | 27026037 |
| intramacrophage infection reinforces the virulence of edwardsiella tarda. | edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. however, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. here, we show that e. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine macrophage model. via pyroptosis, intracellular e. tarda escapes to the extracellular milieu, forming a unique bacterial population. being different from the bacteria cultured alone, this uniqu ... | 2016 | 26953340 |
| histologic and molecular characterization of edwardsiella piscicida infection in largemouth bass (micropterus salmoides). | the genus edwardsiella is composed of a diverse group of facultative anaerobic, gram-negative bacteria that can produce disease in a wide variety of hosts, including birds, reptiles, mammals, and fish. our report describes the isolation and identification of edwardsiella piscicida associated with chronic mortality events in 2 separate captive largemouth bass (micropterus salmoides) populations in new york and florida. wet-mount biopsies of skin mucus, gill, kidney, and spleen from several affect ... | 2016 | 26951328 |
| a prebiotic effect of ecklonia cava on the growth and mortality of olive flounder infected with pathogenic bacteria. | olive flounder (paralichthys olivaceus), also known as the japanese flounder in japan, is one of the most important commercial marine finfish species cultured in korea and japan. the purpose of this study was to evaluate how a species of brown algae (ecklonia cava, e. cava) affects the growth rate of olive flounder and its immune response to pathogenic bacteria. first, the experimental fish were divided into four groups: the control group was fed the diet containing only 1.0% lactobacillus plant ... | 2016 | 26921543 |
| identification and molecular characterization of peroxiredoxin 6 from japanese eel (anguilla japonica) revealing its potent antioxidant properties and putative immune relevancy. | peroxiredoxins (prdx) are thiol specific antioxidant enzymes that play a pivotal role in cellular oxidative stress by reducing toxic peroxide compounds into nontoxic products. in this study, we identified and characterized a peroxiredoxin 6 counterpart from japanese eel (anguilla japonica) (ajprdx6) at molecular, transcriptional and protein level. the identified full-length coding sequence of ajprdx6 (669 bp) coded for a polypeptide of 223 aa residues (24.9 kda). deduced protein of ajprdx6 showe ... | 2016 | 26911410 |
| edwardsiella tarda endocarditis confirmed by indium-111 white blood cell scan: an unusual pathogen and diagnostic modality. | edwardsiella tarda is a freshwater marine member of the family enterobacteriaceae which often colonizes fish, lizards, snakes, and turtles but is an infrequent human pathogen. indium-111- ((111)in-) labeled white blood cell (wbc) scintigraphy is an imaging modality which has a wide range of reported sensitivity and specificity (from 60 to 100% and from 68 to 92%, resp.) for diagnosing acute and chronic infection. we describe a case of suspected e. tarda prosthetic aortic valve and mitral valve e ... | 2016 | 26885418 |
| analysis of the antibacterial effect of an edwardsiella tarda luxs inhibitor. | luxs/ai-2 quorum sensing is involved in the virulence of many bacterial pathogens, including the fish pathogen edwardsiella tarda. in a previous study, we identified a small peptide, 5906, which inhibits e. tarda luxs activity by binding specifically to luxs in a manner that probably prevents the formation of functional luxs homodimer. in the present study, using japanese flounder as the experimental animal, we analyzed the antibacterial effect of 5906 produced by dh5α/p5906 (an escherichia coli ... | 2016 | 26848432 |
| identification and characterization of a novel nod-like receptor family card domain containing 3 gene in response to extracellular atp stimulation and its role in regulating lps-induced innate immune response in japanese flounder (paralichthys olivaceus) head kidney macrophages. | nucleotide oligomerization domain (nod)-like receptor (nlr) family with a caspase activation and recruitment domain (card) containing 3 (nlrc3) protein is an important cytosolic pattern recognition receptor that negatively regulates innate immune response in mammals. hitherto, the immunological significance of nlrc3 protein in fish remains largely uncharacterized. here we identified and characterized a novel nlrc3 gene (named ponlrc3) implicated in regulation of fish innate immunity from japanes ... | 2016 | 26820104 |
| antimicrobial response of galectin-1 from rock bream oplegnathus fasciatus: molecular, transcriptional, and biological characterization. | in this study, we describe the identification and characterization of a proto type galectin, galectin-1, from rock bream oplegnathus fasciatus (ofgal-1). galectins are evolutionarily conserved carbohydrate binding lectins that show a wide range of functions related to development and immune physiology. they have been identified as pattern recognition receptors of innate immune system that recognize a broad range of microbes. ofgal-1 cdna comprised of 993 bp with an open reading frame of 408 bp t ... | 2016 | 26792759 |
| identification of endonuclease domain-containing 1 gene in japanese flounder paralichthys olivaceus. | the mrna level of the endonuclease domain-containing 1 gene (jf_endod1) in japanese flounder paralichthys olivaceus kidney was significantly increased after injection of formalin-killed bacteria cells (fkc) in the previous microarray study. endod1 is a member of the dna/rna non-specific nucleases family, and its role in fish immunity has not been reported. the open reading frame of jf_endod1 cdna was 912 bp, encoding 303 amino acids. the first 27 amino acids were predicted to be a signal peptide ... | 2016 | 26784919 |
| essential oils to control ichthyophthiriasis in pacu, piaractus mesopotamicus (holmberg): special emphasis on treatment with melaleuca alternifolia. | in vitro effect of the melaleuca alternifolia, lavandula angustifolia and mentha piperita essential oils (eos) against ichthyophthirius multifiliis and in vivo effect of m. alternifolia for treating ichthyophthiriasis in one of the most important south american fish, piaractus mesopotamicus (holmberg), were evaluated. the in vitro test consisted of three eos, each at concentrations of 57 μl l(-1) , 114 μl l (-1) , 227 μl l(-1) and 455 μl l (-1) , which were assessed once an hour for 4 h in micro ... | 2016 | 26776242 |
| [expression of edwardsiella tarda luxs gene at different growth stage]. | purpose of this work was to explore the distribution of luxs/ai-2 quorum sensing system in edwardsiella, and analyze expression characteristics and biological function of the key gene luxs accompanying the growth of edwardsiella. | 2015 | 26762033 |
| first comparative characterization of three distinct ferritin subunits from a teleost: evidence for immune-responsive mrna expression and iron depriving activity of seahorse (hippocampus abdominalis) ferritins. | ferritins play an indispensable role in iron homeostasis through their iron-withholding function in living beings. in the current study, cdna sequences of three distinct ferritin subunits, including a ferritin h, a ferritin m, and a ferritin l, were identified from big belly seahorse, hippocampus abdominalis, and molecularly characterized. complete coding sequences (cds) of seahorse ferritin h (haferh), ferritin m (haferm), and ferritin l (haferl) subunits were comprised of 531, 528, and 522 bas ... | 2016 | 26747640 |
| edwardsiella tarda bacteremia. a rare but fatal water- and foodborne infection: review of the literature and clinical cases from a single centre. | edwardsiella tarda bacteremia (etb) can be a fatal disease in humans. | 2017 | 26744588 |
| bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology. | bacteriophages have been proposed as an alternative to antibiotic usage and several studies on their application in aquaculture have been reported. this review highlights progress to date on phage therapies for the following fish and shellfish diseases and associated pathogens: hemorrhagic septicemia (aeromonas hydrophila) in loaches, furunculosis (aeromonas salmonicida) in trout and salmon, edwardsiellosis (edwardsiella tarda) in eel, columnaris disease (flavobacterium columnare) in catfish, ra ... | 2017 | 26713223 |
| construction and immune protection evaluation of recombinant polyvalent ompas derived from genetically divergent ompa by dna shuffling. | a wide variety of bacterial infections is a major challenge in aquaculture. development of polyvalent vaccines that can fight against as many pathogens as possible is especially necessary. the present study uses dna shuffling to create a new hybrid ompa with improved cross-protection against vibrio alginolyticus and edwardsiella tarda through the recombination of six ompa genes from vibrio parahaemolyticus, v. alginolyticus, e. tarda and escherichia coli. out of the 43 recombinant chimeras genes ... | 2016 | 26707781 |
| coagulation factor ii from rock bream (oplegnathus fasciatus): first report on the molecular biological function and expression analysis in the teleost. | the rapid haemostasis of fish prevents bleeding or infection that could be caused by physical properties of the aquatic environment. additionally, the innate immune system is the first line of defence against infection and is responsible for the recognition of pathogen-associated molecular patterns, which are important for the activation of acquired immune responses. coagulation factor ii (cfii) is an important factor in the coagulation system and is involved in recognition and interaction with ... | 2016 | 26626585 |
| dietary bacillus subtilis fptb13 and chitin, single or combined, modulate systemic and cutaneous mucosal immunity and resistance of catla, catla catla (hamilton) against edwardsiellosis. | effects of dietary administration of bacillus subtilis fptb13 and chitin, single or combined, on the systemic immunity, mucosal immunity and resistance of catla (catla catla) against edwardsiella tarda infection were investigated. the probiotic attributes of b. subtilis was tested by conducting antagonism study, safety in catla, in vitro immunomodulation and dietary immunomodulation. results of these studies indicated the probiotic potential of the strain. from the preliminary dietary immunomodu ... | 2015 | 26616655 |
| a galectin related protein from oplegnathus fasciatus: genomic, molecular, transcriptional features and biological responses against microbial pathogens. | galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. here, we report a novel galectin related protein (grp) identified from rock bream (oplegnathus fasciatus), designated ofgal like b. the cdna of ofgal like b is 517 bp with an open reading frame (orf) of 438 bp, encoding 145 amino acids, with a s ... | 2016 | 26615008 |
| molecular, genomic, and expressional delineation of a piscidin from rock bream (oplegnathus fasciatus) with evidence for the potent antimicrobial activities of of-pis1 peptide. | the piscidin family comprises a group of antimicrobial peptides (amps) that are vital components of teleost innate immunity. piscidins protect the host from pathogens, through multifaceted roles as immunomodulators and anti-infective peptides. the present study reports the identification, and characterization of a putative piscidin homolog, of-pis1, from rock bream (oplegnathus fasciatus). a combined genomic and transcriptomic approach revealed that the of-pis1 gene comprises 1396 nucleotides (n ... | 2016 | 26549174 |
| analysis of zebrafish (danio rerio) behavior in response to bacterial infection using a self-organizing map. | animal behavioral responses have been recently established as a suitable tool for detecting contaminants in the environment for risk assessment in situ. in this study, we observed movement behavior of zebrafish (danio rerio) before and after infection with edwardsiella tarda ck41 for 3 days until death. | 2015 | 26497220 |
| immune responses of flounder paralichthys olivaceus vaccinated by immersion of formalin-inactivated edwardsiella tarda following hyperosmotic treatment. | the aim of the present study was to evaluate the effects of hyperosmotic immersion (hi) vaccination and determine the optimum hyperosmotic salinity for flounder paralichthys olivaceus by investigating its immune responses following vaccination. flounder were immersed in 1 of 3 hyperosmotic solutions at 50, 60 and 70‰ salinity, then transferred into 30‰ salinity normal seawater containing formalin-inactivated edwardsiella tarda for vaccination (3 hi groups), or were immersed in normal seawater as ... | 2015 | 26480914 |
| first description of programmed cell death10 (pdcd10) in rock bream (oplegnathus fasciatus): potential relations to the regulation of apoptosis by several pathogens. | in this study, we isolated and characterized programmed cell death10 (pdcd10), which is known to be related to apoptosis, from rock bream (oplegnathus fasciatus). the full-length rock bream pdcd10 (rbpdcd10) cdna (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among the other pdcd10 sequences tested. rbpdcd10 was significantly expressed in the liver, rbc (red blood c ... | 2016 | 26472617 |
| edwardsiella tarda esce (orf13 protein) is a type iii secretion system-secreted protein that is required for the injection of effectors, secretion of translocators, and pathogenesis in fish. | the type iii secretion system (t3ss) of edwardsiella tarda is crucial for its intracellular survival and pathogenesis in fish. the orf13 gene (esce) of e. tarda is located 84 nucleotides (nt) upstream of esrc in the t3ss gene cluster. we found that esce is secreted and translocated in a t3ss-dependent manner and that amino acids 2 to 15 in the n terminus were required for a completely functional t3ss in e. tarda. deletion of esce abolished the secretion of t3ss translocators, as well as the secr ... | 2015 | 26459509 |
| gene expression and functional characterization of serum amyloid p component 2 in rock bream, oplegnathus fasciatus. | mammalian serum amyloid p component (sap) recognizes a wide range of exogenous pathogenic substances and activates a complementary pathway leading to pathogen clearance. to determine the potential roles of sap in the fish immune system, sap (rbsap2) gene was cloned from ests analysis of rock bream (oplegnathus fasciatus), which consisted of a signal peptide and pentraxin domain. phylogenetic analysis revealed that the rbsap2 gene was classified with other known fish saps. rbsap2 was highly expre ... | 2015 | 26455663 |
| a multiplex pcr for the simultaneous detection of tenacibaculum maritimum and edwardsiella tarda in aquaculture. | a specific and sensitive multiplex pcr (mpcr) method was developed as a useful tool for the simultaneous detection of two important flatfish pathogens in marine aquaculture, tenacibaculum maritimum and edwardsiella tarda. in fish tissues, the average detection limit for these mpcr-amplified organisms was 2 × 10 ⁵ ± 0.2 cfu/g and 4 × 10 ⁵ ± 0.3 cfu/g, respectively. these values are similar or even lower than those previously obtained using the corresponding single pcr. moreover, mpcr did not prod ... | 2014 | 26418855 |
| pharmacokinetics, pharmacokinetic-pharmacodynamic relationship, and withdrawal period of amoxicillin sodium in olive flounder (paralichthys olivaceus). | 1. the pharmacokinetics (pk) and withdrawal period of amoxicillin sodium in olive flounder and its activity against pathogenic bacteria of olive flounder were investigated. 2. intramuscular administration (12.5 or 125 mg/kg, n = 160) and hplc analysis of sera were used. 3. rapid absorption (tmax 2.6 and 2.2 h), prolonged action (terminal half-life, 15.52 and 10.42 h; mrt, 18.79 and 14.44 h), and dose-proportional exposure (auc0-∞, 273.69 and 2755.37 h. μg/ml) were observed after 12.5 and 125 mg/ ... | 2016 | 26407038 |
| live edwardsiella tarda vaccine enhances innate immunity by metabolic modulation in zebrafish. | control of bacterial infection resides in the core of human health and sustainable animal breeding. vaccines as an economic and efficient immunoprophylaxis have been widely accepted, but mechanisms for vaccines do not fully understand. information regarding to metabolome in response to vaccines is not available. here we explore the metabolic features by using gc/ms based metabolic profile and trace metabolic mechanisms in zebrafish (dario rerio) in response to live edwardsiella tarda vaccine. pa ... | 2015 | 26394266 |
| gene structure, molecular characterization and transcriptional expression of two p38 isoforms (mapk11 and mapk14) from rock bream (oplegnathus fasciatus). | the p38 kinases are one of the four subgroups of mitogen-activated protein kinase (mapk) superfamily which are involved in the innate immunity. the p38 subfamily that includes four members namely p38α (mapk14), p38β (mapk11), p38γ (mapk12) and p38δ (mapk13), regulates the activation of several transcription factors. in this study, a p38β (ofmapk11) homolog and a p38α (ofmapk14) homolog of oplegnathus fasciatus were identified at genomic level. results clearly showed that both mapk11 and mapk14 a ... | 2015 | 26363230 |
| molecular characterization of purinergic receptor p2x4 involved in japanese flounder (paralichthys olivaceus) innate immune response and its interaction with atp release channel pannexin1. | p2x4 receptor (p2x4r) is a member of trimeric atp-gated receptor channel family. despite the importance of p2x4r in innate immunity has been addressed in mammals, the immunological significance of p2x4r has not been characterized in fish. in the present study we identified a full-length p2x4r cdna sequence from japanese flounder paralichthys olivaceus (termed pop2x4r) by rt-pcr and race approaches and analyzed its gene expression patterns under normal and immune challenge conditions. qualitative ... | 2015 | 26321132 |
| enhanced immune response and resistance to edwardsiellosis following dietary chitooligosaccharide supplementation in the olive flounder (paralichthys olivaceus). | this study was conducted to evaluate the effects of dietary chitooligosaccharide (cos) supplementation on peripheral leukocyte count, head kidney leukocyte phagocytic rate, phagocytic index, respiratory burst activity, serum lysozyme activity, and immune protection in paralichthys olivaceus. a total of 300 flounder with an average body weight of 80-100 g were randomly assigned into four dietary groups: (i) basic diet (control), basic diet containing (ii) 0.5% cos, and (iii) 1% cos, fed continuou ... | 2015 | 26314523 |
| roles of rpos in yersinia pseudotuberculosis stress survival, motility, biofilm formation and type vi secretion system expression. | rpos (σ(s)), the stationary phase/stress σ factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. however, the role of rpos appears to differ in different bacteria. while rpos is an important regulator of flagellum biosynthesis, it is associated with biofilm development in edwardsiella tarda. biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. the type vi secretion syst ... | 2015 | 26310305 |
| recombinant sialidase nana (rnana) cleaves α2-3 linked sialic acid of host cell surface n-linked glycoprotein to promote edwardsiella tarda infection. | edwardsiella tarda is one of the major pathogenic bacteria affecting both marine and freshwater fish species. sialidase nana expressed endogenously in e. tarda is glycosidase removing sialic acids from glycoconjugates. recently, the relationship of nana sialidase activity to e. tarda infection has been reported, however, the mechanism with which sialidase nana aids the pathogenicity of e. tarda remained unclear. here, we comprehensively determined the biochemical properties of nana towards vario ... | 2015 | 26291491 |
| characterization of rock bream (oplegnathus fasciatus) complement components c1r and c1s in terms of molecular aspects, genomic modulation, and immune responsive transcriptional profiles following bacterial and viral pathogen exposure. | the complement components c1r and c1s play a crucial role in innate immunity via activation of the classical complement cascade system. as initiators of the pathogen-induced signaling cascade, c1r and c1s modulate innate immunity. in order to understand the immune responses of teleost c1r and c1s, oplegnathus fasciatus c1r and c1s genes (ofc1r and ofc1s) were identified and characterized. the genomic sequence of ofc1r was enclosed with thirteen exons that represented a putative peptide with 704 ... | 2015 | 26241508 |
| first report of edwardsiellosis in cage-cultured sharpsnout sea bream, diplodus puntazzo from the mediterranean. | edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. the bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic edwardsiella from those isolated from humans by placing them in a new species, e. piscicida. here we report the first case of edwardsiellosis in cultured sharpsnout sea breams, diplodus puntazzo in greece. | 2015 | 26193880 |
| starvation beneficially influences the liver physiology and nutrient metabolism in edwardsiella tarda infected red sea bream (pagrus major). | dietary compromises, especially food restrictions, possess species-specific effects on the health status and infection control in several organisms, including fish. to understand the starvation-mediated physiological responses in edwardsiella tarda infected red sea bream, especially in the liver, we performed a 20-day starvation experiment using 4 treatment (2 fed and 2 starved) groups, namely, fed-placebo, starved-placebo, fed-infected, and starved-infected, wherein bacterial exposure was done ... | 2015 | 26188170 |
| endoscopic ultrasonography-guided transmural drainage of an infected hepatic cyst due to edwardsiella tarda: a case report. | infected hepatic cysts are very rare compared to simple liver cysts and abscesses. we treated a 77-year-old man with an infected hepatic cyst in the lateral segment caused by edwardsiella tarda, which has not been previously reported as a pathogenic organism associated with infected hepatic cysts. percutaneous drainage was temporarily effective, but infection recurred after the drainage tube was removed. we then inserted two drainage tubes into the cyst using an endoscopic ultrasonography (eus)- ... | 2014 | 26184023 |
| immune response of flounder (paralichthys olivaceus) was associated with the concentration of inactivated edwardsiella tarda and immersion time. | in the optimization of immersion strategy, vaccine concentration and immersion time are two key factors needed to be considered, which largely determined the immune efficacy. in this work, the healthy flounder were vaccinated in formalin-killed edwardsiella tarda at four concentrations (10(6), 10(7), 10(8), 10(9) cfu ml(-1)) for three immersion times (30, 60, 90 min), respectively. at the 6th week post vaccination, the flounders were challenged with live e. tarda, and the relative percent surviv ... | 2015 | 26163937 |
| a homolog of kunitz-type serine protease inhibitor from rock bream, oplegnathus fasciatus: molecular insights and transcriptional modulation in response to microbial and pamp stimulation, and tissue injury. | serine proteases and their inhibitors play vital roles in diverse biological processes. in this study, we identified and characterized cdna coding for a kunitz-type serine protease inhibitor (spi), which we designated as rbkspi, in a commercially important species, rock bream. the full-length cdna sequence of rbkspi consisted of 2452 bp with an open reading frame (orf) of 1521 bp encoding a polypeptide of 507 amino acid (aa) residues. in the rbkspi protein, manec, pkd, ldla, and two kunitz domai ... | 2015 | 26162478 |
| identification and expression analysis of nascent polypeptide-associated complex alpha gene in response to immune challenges in japanese flounder paralichthys olivaceus. | nascent polypeptide-associated complex (nac) is a conserved heterodimeric protein consisting of alpha and beta subunits. in addition to acting as a protein translation chaperone by forming a heterodimer with the beta subunit, nac alpha (naca) also shows important immune significance independent of nac beta in mammalian cells. in lower vertebrates, however, the immunological relevance of naca has not been revealed yet. in the present study, we identified and characterized a naca gene (termed pona ... | 2015 | 26142144 |
| type iii secretion system translocon component eseb forms filaments on and mediates autoaggregation of and biofilm formation by edwardsiella tarda. | the type iii secretion system (t3ss) of edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. eseb, a component required for effector translocation, is reported to mediate autoaggregation of e. tarda. in this study, we demonstrate that eseb forms filamentous appendages on the surface of e. tarda and is required for biofilm formation by e. tarda in dulbecco's modified eagle's medium (dmem). biofilm formation by e. tarda in dmem does not requir ... | 2015 | 26116669 |
| complete genome sequence of edwardsiella tarda isolate fl95-01, recovered from channel catfish. | edwardsiella tarda is a gram-negative facultative anaerobe that has been isolated from fish, reptiles, amphibians, and mammals, including humans. this is a report of the complete and annotated genome of isolate fl95-01, recovered from channel catfish (ictalurus punctatus). | 2015 | 26112788 |
| insights into the virulence-related genes of edwardsiella tarda isolated from turbot in europe: genetic homogeneity and evidence for vibrioferrin production. | edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. insights gained on e. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreaks. in this report, we have established the correlation between the presence of virulence genes, related with three aspects typically involved in bacterial pathogenesis (chondroitinase activity, quorum sensing ... | 2016 | 26096159 |
| effects of protein hydrolysates supplementation in low fish meal diets on growth performance, innate immunity and disease resistance of red sea bream pagrus major. | this study was conducted to evaluate the supplemental effects of three different types of protein hydrolysates in a low fish meal (fm) diet on growth performance, feed utilization, intestinal morphology, innate immunity and disease resistance of juvenile red sea bream. a fm-based diet was used as a high fish meal diet (hfm) and a low fish meal (lfm) diet was prepared by replacing 50% of fm by soy protein concentrate. three other diets were prepared by supplementing shrimp, tilapia or krill hydro ... | 2015 | 26074096 |
| generation of killed but metabolically active (kbma) edwardsiella tarda and evaluation of its potential as a protective vaccine. | a technology for inactivation of pathogens in human blood products by treatment with amotosalen hydrochloride (s-59) in combination with long wavelength ultraviolet light (uva) to decrease transfusion-mediated sepsis has been applied to make safe vaccines against human pathogenic bacteria, and the resultants were called killed but metabolically active (kbma) bacteria. in the present study, we first generated kbma edwardsiella tarda and evaluated its potential as a protective vaccine in olive flo ... | 2015 | 26074095 |
| metabolome strategy against edwardsiella tarda infection through glucose-enhanced metabolic modulation in tilapias. | edwardsiella tarda causes fish disease and great economic loss. however, metabolic strategy against the pathogen remains unexplored. in the present study, gc-ms based metabolomics was used to investigate the metabolic profile from tilapias infected by sublethal dose of e. tarda. the metabolic differences between the dying group and survival group allow the identification of key pathways and crucial metabolites during infections. more importantly, those metabolites may modulate the survival-relat ... | 2015 | 26057462 |
| ontogeny, tissue distribution and expression analysis of igz in rohu, labeo rohita in response to various stimuli. | this study reports presence of igz transcripts, its ontogeny, tissue distribution and expression following various stimulations/infections in rohu. the derived rohu igz sequence clustered together with igz of ctenopharyngodon idella and cyprinus carpio in phylogenetic analysis. igz expression was detected at early developmental stages with higher expression at 1 day post-fertilization, and higher in anterior kidney and lower in skin tissues of juveniles. an inductive expression of igz was observ ... | 2015 | 26055084 |
| full-genome sequence of a novel myovirus, gf-2, infecting edwardsiella tarda: comparison with other edwardsiella myoviral genomes. | edwardsiellosis, which is caused by edwardsiella tarda, a gram-negative bacterium, is one of the most serious infectious diseases in both marine and freshwater fish farms worldwide. previously, we reported the complete genome sequences of three e. tarda-lytic bacteriophages (two podoviruses and a myovirus), which were isolated from fish tissues and fish-rearing seawater. further genomic information regarding e. tarda phages is important for understanding phage-host interactions as well as for ap ... | 2015 | 26049743 |
| in vivo programmed gene expression based on artificial quorum networks. | the quorum sensing (qs) system, as a well-functioning population-dependent gene switch, has been widely applied in many gene circuits in synthetic biology. in our work, an efficient cell density-controlled expression system (qs) was established via engineering of the vibrio fischeri luxi-luxr quorum sensing system. in order to achieve in vivo programmed gene expression, a synthetic binary regulation circuit (araqs) was constructed by assembling multiple genetic components, including the quorum q ... | 2015 | 25979894 |
| comparative proteomic study of edwardsiella tarda strains with different degrees of virulence. | edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. this species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. in this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the norwest of spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. one h ... | 2015 | 25979771 |
| comparative analysis of adaptive immune response after vaccine trials using live attenuated and formalin-killed cells of edwardsiella tarda in ginbuna crucian carp (carassius auratus langsdorfii). | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. although vaccine trials with formalin-killed cells (fkc) have been reported, the vaccinations failed in protect against e. tarda infection. on the other hand, a live attenuated vaccine strategy is effective against edwardsiellosis; however, the mechanism underlying its effectiveness in fish is unclear. in the present study, we compared the adaptive immune responses in fish vaccinated with fkcs and live attenuate ... | 2015 | 25959574 |
| first molecular characterisation and expression analysis of a teleost thioredoxin-interacting protein (txnip) gene from rock bream (oplegnathus fasciatus). | thioredoxin-interacting protein (txnip) is an important regulator of glucose metabolism that functions by inhibiting cellular glucose uptake. the full-length rock bream (oplegnathus fasciatus) txnip (rbtxnip) cdna (2499 bp) contains an open reading frame of 1188 bp encoding 396 amino acids. furthermore, multiple alignments showed that the arrestin domain was well conserved among the other txnip sequences tested. rbtxnip was predicted to contain a pxxp and ppxy motif. phylogenetic analysis indica ... | 2015 | 25934185 |
| a potential antioxidant enzyme belonging to the atypical 2-cys peroxiredoxin subfamily characterized from rock bream, oplegnathus fasciatus. | peroxiredoxins (prxs), a diverse family of antioxidant enzymes, exert their antioxidant function through which different peroxide species are detoxified. this study describes both structural and functional characterization of a mitochondrial prx identified in rock bream, oplegnathus fasciatus (rbprx5). the orf (573 bp) of rbprx5 encoded a protein of 190 amino acids (20 kda) containing a putative mitochondrial targeting sequence (residues 1-20) and a thioredoxin-2 motif (residues 31-190) and thre ... | 2015 | 25934084 |
| functional characterisation and expression analysis of recombinant serum amyloid p isoform 1 (rbsap1) from rock bream (oplegnathus fasciatus). | lectins are carbohydrate-binding proteins that play important roles in the recognition and elimination of pathogens via the innate immune system. pentraxins (ptx) are humoral lectins, which are multifunctional proteins in vertebrates. pentraxins can be divided into two groups based on their primary structure: short (c-reactive protein and serum amyloid p [sap]) and long pentraxins (ptx3 and neuronal pentraxins). previously, sap was shown to have ca(2+)-dependent binding specificity for various l ... | 2015 | 25917975 |
| edwardsiella tarda sip1: a serum-induced zinc metalloprotease that is essential to serum resistance and host infection. | edwardsiella tarda is a severe bacterial pathogen to a wide arrange of farmed fish. one salient virulent feature of e. tarda is a remarkable ability to survive in host serum. in this study, in order to identify e. tarda proteins involved in serum resistance, we conducted proteomic analysis to examine the extracellular protein profiles of tx01, a pathogenic e. tarda isolate, in response to serum treatment. five differentially expressed proteins were identified, one of which was a putative zinc pr ... | 2015 | 25899393 |
| edwardsiella tarda-regulated proteins in japanese flounder (paralichthys olivaceus): identification and evaluation of antibacterial potentials. | edwardsiella tarda is a severe aquaculture pathogen that infects a wide range of fish including japanese flounder (paralichthys olivaceus). in this study, proteomic analysis was conducted to examine flounder spleen proteins altered in expression during e. tarda infection. twenty differentially expressed proteins were identified, which belonged to five functional categories. five upregulated proteins, i.e. calmodulin (cam), cathepsin l (catl), calreticulin (crt), ferritin middle subunit (ferm), a ... | 2015 | 25896741 |
| a quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. | delivery of antigens by live bacterial carriers can elicit effective humoral and cellular responses and may be an attractive strategy for live bacterial vaccine production through introduction of a vector that expresses an exogenous protective antigen. to overcome the instability and metabolic burden associated with plasmid introduction, alternative strategies, such as the use of in vivo-inducible promoters, have been proposed. however, screening an ideal in vivo-activated promoter with high eff ... | 2015 | 25888727 |
| fructose restores susceptibility of multidrug-resistant edwardsiella tarda to kanamycin. | edwardsiella tarda, the causative agent of edwardsiellosis, imposes medical challenges in both the clinic and aquaculture. the emergence of multidrug resistant strains makes antibiotic treatment impractical. the identification of molecules that facilitate or promote antibiotic efficacy is in high demand. in the present study, we aimed to identify small molecules whose abundance is correlated with kanamycin resistance in e. tarda by gas chromatography-mass spectrometry. we found that the abundanc ... | 2015 | 25675328 |
| identification and functional characterization of the novel edwardsiella tarda effector esej. | edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. the type iii secretion system (t3ss) of e. tarda has been identified as a key virulence factor that contributes to pathogenesis in fish. however, little is known about the associated effectors translocated by this t3ss. in this study, by comparing the profile of secreted proteins of the wild-type ppd130/91 and its t3ss atpase δesan mutant, we ide ... | 2015 | 25667268 |
| exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. | multidrug-resistant bacteria are an increasingly serious threat to human and animal health. however, novel drugs that can manage infections by multidrug-resistant bacteria have proved elusive. here we show that glucose and alanine abundances are greatly suppressed in kanamycin-resistant edwardsiella tarda by gc-ms-based metabolomics. exogenous alanine or glucose restores susceptibility of multidrug-resistant e. tarda to killing by kanamycin, demonstrating an approach to killing multidrug-resista ... | 2015 | 25651179 |
| real-time polymerase chain reaction assays for the detection and quantification of edwardsiella tarda, edwardsiella piscicida, and edwardsiella piscicida-like species in catfish tissues and pond water. | researchers have proposed the adoption of 3 distinct genetic taxa among bacteria previously classified as edwardsiella tarda; namely e. tarda, e. piscicida, and a taxon presently termed e. piscicida-like. individual real-time polymerase chain reaction (qpcr) assays were developed, based on published primers, for e. tarda, e. piscicida, and e. piscicida-like sp. to provide rapid quantitative confirmatory tests for these phenotypically ambiguous bacteria. the qpcr assays were shown to be repeatabl ... | 2015 | 25613040 |
| characterization of ampicillin-stressed proteomics and development of a direct method for detecting drug-binding proteins in edwardsiella tarda. | antibiotic-resistant edwardsiella tarda poses a severe challenge to aquaculture. an understanding for antibiotic-resistant mechanisms is crucial to control the disease. the present study characterizes e. tarda ampicillin-stressed proteome and shows the importance of energy metabolism including the tca cycle and glycolysis/gluconeogenesis in the antibiotic resistance. further combination with antibiotic measurement develops a new method for identification of antibiotic-binding proteins out of dif ... | 2015 | 25596334 |
| edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway. | edwardsiella tarda is a gram-negative bacterium with a broad host range that includes a wide variety of farmed fish as well as humans. e. tarda has long been known to be able to survive in host serum, but the relevant mechanism is unclear. in this study, we investigated the fundamental question, i.e. whether e. tarda activated serum complement or not. we found that (i) when incubated with flounder serum, e. tarda exhibited a high survival rate (87.6%), which was slightly but significantly reduce ... | 2015 | 25575477 |
| insights into molecular profiles and genomic evolution of an irak4 homolog from rock bream (oplegnathus fasciatus): immunogen- and pathogen-induced transcriptional expression. | as a pivotal signaling mediator of toll-like receptor (tlr) and interleukin (il)-1 receptor (il-1r) signaling cascades, the il-1r-associated kinase 4 (irak4) is engaged in the activation of host immunity. this study investigates the molecular and expressional profiles of an irak4-like homolog from oplegnathus fasciatus (ofirak4). the ofirak4 gene (8.2 kb) was structured with eleven exons and ten introns. a putative coding sequence (1395bp) was translated to the ofirak protein of 464 amino acids. ... | 2015 | 25555811 |
| mitochondrial peroxiredoxin 3 (prx3) from rock bream (oplegnathus fasciatus): immune responses and role of recombinant prx3 in protecting cells from hydrogen peroxide induced oxidative stress. | pathogenic infections and environmental factors cause a variety of stresses in fish including oxidative stress by rapid elevation of reactive oxygen species (ros) and reactive nitrogen species (rns). transcriptional activation and expression of antioxidant enzymes are essential for reducing the oxidative stress. in this study, we present the molecular characterization, immune responses and ros scavenging activity of mitochondrial peroxiredoxin 3 from oplegnathus fasciatus (rbprx3). coding sequen ... | 2015 | 25542382 |
| identification and characterization of the cdnas encoding the two subunits of chinese mitten crab (eriocheir sinensis) calcineurin: their implications in stress and immune response. | calcineurin (cn), the only ca(2+)/calmodulin-activated serine/threonine protein phosphatase, is a key effector participating in ca(2+)-dependent signal transduction pathways in a number of cellular processes under normal, stress and pathological conditions. however, the expression and the relevance of cn in stress and immune response have not been characterized in crustaceans. here, we identified the cdnas that encode the two subunits of cn (termed escn-a and escn-b, respectively) in chinese mit ... | 2015 | 25541080 |
| generation and evaluation of virulence attenuated mutants of edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder (paralichthys olivaceus). | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. the development of a live attenuated vaccine may be an effective approach for preventing this disease in fish. in this study, we introduced deletions of esrb, esac, evph, rpos, and pura into the e. tarda lse40δaroa strain, thereby generating five double-gene mutants (δaroaδesrb, δaroaδesac, δaroaδrpos, δaroaδevph, and δaroaδpura) and two triple-gene mutants (δaroaδesrbδevph and δaroaδesacδevph). when blue gouram ... | 2015 | 25541077 |
| higher antiviral response of rig-i through enhancing rig-i/mavs-mediated signaling by its long insertion variant in zebrafish. | as an intracellular pattern recognition receptor (prr), the retinoic acid-inducible gene-i (rig-i) is responsible for the recognition of cytosolic viral nucleic acids and the production of type i interferons (ifns). in the present study, an insertion variant of rig-i with 38 amino acids inserted in the n-terminal card2 domain, as well as the typical type, named as rig-ia and rig-ib respectively were identified in zebrafish. rig-ia and rig-ib were all up-regulated following the infection of a neg ... | 2015 | 25524497 |
| adhesive and invasive capacities of edwardsiella tarda isolated from south american sea lion. | edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. although e. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. a survey of zoonotic intestinal bacteria in fresh feces from south american sea lions (sasl) otaria flavescens, reported e. tarda as the most frequently isolated species. in this study, we used hep-2 cells to establish in vitro the adherence and invasi ... | 2014 | 25477948 |
| enterobacteria identification and detection of diarrheagenic escherichia coli in a port complex. | the port complex of maranhão (pcm) is the second largest port complex in brazil, receiving ships with large volumes of ballast water. to evaluate the microbiological quality of its waters, physicochemical parameters (ph and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic escherichia coli strains were analyzed. in order to identify the presence of e. coli virulence genes target regions of the stx, elt, est, aggr, cvd432, ipah ... | 2014 | 25477930 |
| phylogenomics characterization of a highly virulent edwardsiella strain et080813(t) encoding two distinct t3ss and three t6ss gene clusters: propose a novel species as edwardsiella anguillarum sp. nov. | as important zoonotic organisms causing infections in humans, edwardsiella bacteria are also notorious leading fish pathogens haunting worldwide aquaculture industries. however, the taxa are now widely recognized to be misclassified, which hurdled the understanding of the epidemiology and development of effective diagnostics and vaccines. currently the genus edwardsiella consists of three species edwardsiella tarda, e. ictaluri, and e. hoshinae. previous phylogenomic analysis revealed that e. ta ... | 2015 | 25466920 |