Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| neisseria gonorrhoeae : detection and typing by probe hybridization, lcr, and pcr. | neisseria gonorrhoeae, first described by neisser in 1879, is a gram-negative, nonmotile, nonspore-forming diplococcus, belonging to the family neisseriaceae. it is the etiologic agent of gonorrhea. the other pathogenic species is neisseria meningitidis, to which n. gonorrhoeae is genetically closely related. although n. meningitidis is not usually considered to be a sexually transmitted disease, it may infect the mucous membranes of the anogenital area of homosexual men (1). the other members o ... | 1999 | 21390726 |
| first report of performance of the versant ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae. | we evaluated the analytical, work flow, and clinical performance of the versant ct/gc dna 1.0 assay (versant ct/gc assay, where "ct" represents chlamydia trachomatis and "gc" represents neisseria gonorrhoeae). the assay simultaneously detects chlamydia trachomatis and neisseria gonorrhoeae in swab and first-catch urine (fcu) specimens. the limit of detection (lod) was determined to be 342 copies/ml for c. trachomatis and 137 copies/ml for gc. the versant ct/gc assay detected 15 c. trachomatis se ... | 2011 | 21307209 |
| a novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and dna viral systems. | the use of nucleases as toxins for defense, offense or addiction of selfish elements is widely encountered across all life forms. using sensitive sequence profile analysis methods, we characterize a novel superfamily (the sukh superfamily) that unites a diverse group of proteins including smi1/knr4, pgs2, fbxo3, skip16, syd, herpesviral us22, irs1 and trs1, and their bacterial homologs. using contextual analysis we present evidence that the bacterial members of this superfamily are potential imm ... | 2011 | 21306995 |
| independent evolution of the core and accessory gene sets in the genus neisseria: insights gained from the genome of neisseria lactamica isolate 020-06. | the genus neisseria contains two important yet very different pathogens, n. meningitidis and n. gonorrhoeae, in addition to non-pathogenic species, of which n. lactamica is the best characterized. genomic comparisons of these three bacteria will provide insights into the mechanisms and evolution of pathogenesis in this group of organisms, which are applicable to understanding these processes more generally. | 2010 | 21092259 |
| the biotin requirements of neisseria sicca. | 1946 | 20992394 | |
| functional analysis of the carbohydrate-binding module of an esterase from neisseria sicca sb involved in the degradation of cellulose acetate. | an esterase gene from neisseria sicca sb encoding caea, which catalyzes the deacetylation of cellulose acetate, was cloned. caea contained a putative catalytic domain of carbohydrate esterase family 1 and a carbohydrate-binding module (cbm) family 2. we constructed two derivatives, with and without the cbm of caea. binding assay indicated that the cbm of caea had an affinity for cellulose. | 2010 | 20834142 |
| prevalence of chlamydia infection among women visiting a gynaecology outpatient department: evaluation of an in-house pcr assay for detection of chlamydia trachomatis. | screening women for chlamydia trachomatis infection in developing countries is highly desirable because of asymptomatic infection. the existing diagnostic methods in developing countries are not effective and their sensitivity fall below 45.0% which leads to further spread of infection. there is an urgent need for improved and cost effective diagnostic tests that will reduce the burden of sexually transmitted infections in the developing world. | 2010 | 20822551 |
| genome sequencing reveals widespread virulence gene exchange among human neisseria species. | commensal bacteria comprise a large part of the microbial world, playing important roles in human development, health and disease. however, little is known about the genomic content of commensals or how related they are to their pathogenic counterparts. the genus neisseria, containing both commensal and pathogenic species, provides an excellent opportunity to study these issues. we undertook a comprehensive sequencing and analysis of human commensal and pathogenic neisseria genomes. commensals h ... | 2010 | 20676376 |
| treatment of allergic diseases: application to clinical practice of a new concept of mutual substitutions of antibody molecules on the surface of mast cells. | 2007 | 20525152 | |
| identification of a siderophore utilization locus in nontypeable haemophilus influenzae. | haemophilus influenzae has an absolute aerobic growth requirement for either heme, or iron in the presence of protoporphyrin ix. both iron and heme in the mammalian host are strictly limited in their availability to invading microorganisms. many bacterial species overcome iron limitation in their environment by the synthesis and secretion of small iron binding molecules termed siderophores, which bind iron and deliver it into the bacterial cell via specific siderophore receptor proteins on the b ... | 2010 | 20398325 |
| conjugative plasmids of neisseria gonorrhoeae. | many clinical isolates of the human pathogen neisseria gonorrhoeae contain conjugative plasmids. the host range of these plasmids is limited to neisseria species, but presence of a tetracycline (tetm) determinant inserted in several of these plasmids is an important cause of the rapid spread of tetracycline resistance. previously plasmids with different backbones (dutch and american type backbones) and with and without different tetm determinants (dutch and american type tetm determinants) have ... | 2010 | 20376355 |
| characterization of slackia exigua isolated from human wound infections, including abscesses of intestinal origin. | eleven clinical strains isolated from infected wound specimens were subjected to polyphasic taxonomic analysis. sequence analysis of the 16s rrna gene showed that all 11 strains were phylogenetically related to slackia exigua. additionally, conventional and biochemical tests of 6 of the 11 strains were performed as supplementary methods to obtain phenotypic identification by comparison with the phenotypes of the relevant type strains. s. exigua has been considered an oral bacterial species in th ... | 2010 | 20107092 |
| development of a rapid automated influenza a, influenza b, and respiratory syncytial virus a/b multiplex real-time rt-pcr assay and its use during the 2009 h1n1 swine-origin influenza virus epidemic in milwaukee, wisconsin. | rapid, semiautomated, and fully automated multiplex real-time rt-pcr assays were developed and validated for the detection of influenza (flu) a, flu b, and respiratory syncytial virus (rsv) from nasopharyngeal specimens. the assays can detect human h1n1, h3n2, and swine-origin (s-oiv) h1n1 flu a viruses and were effectively used to distinguish flu a infections (of all subtypes) from flu b and rsv infections during the current s-oiv outbreak in milwaukee, wi. the analytical limits of detection we ... | 2010 | 19959800 |
| [neisseria sicca]. | 2009 | 19915756 | |
| biochemical and genomic analysis of the denitrification pathway within the genus neisseria. | since neisseria gonorrhoeae and neisseria meningitidis are obligate human pathogens, a comparison with commensal species of the same genus could reveal differences important in pathogenesis. the recent completion of commensal neisseria genome draft assemblies allowed us to perform a comparison of the genes involved in the catalysis, assembly and regulation of the denitrification pathway, which has been implicated in the virulence of several bacteria. all species contained a highly conserved nitr ... | 2009 | 19762442 |
| single-residue changes in the c-terminal disulfide-bonded loop of the pseudomonas aeruginosa type iv pilin influence pilus assembly and twitching motility. | pila, the major pilin subunit of pseudomonas aeruginosa type iv pili (t4p), is a principal structural component. pila has a conserved c-terminal disulfide-bonded loop (dsl) that has been implicated as the pilus adhesinotope. structural studies have suggested that dsl is involved in intersubunit interactions within the pilus fiber. pila mutants with single-residue substitutions, insertions, or deletions in the dsl were tested for pilin stability, pilus assembly, and t4p function. mutation of eith ... | 2009 | 19717595 |
| neisseria sicca/subflava bacteremia presenting as cutaneous nodules in an immunocompromised host. | neisseria sicca/subflava are generally considered commensal inhabitants of the human oropharynx. we describe a case of disseminated n. sicca/subflava infection in an immunocompromised 15-year-old male presenting with cutaneous erythematous nodules. our report adds to the growing evidence that these bacteria can cause disseminated infections, and describes a cutaneous manifestation of disseminated disease with n. sicca/subflava. | 2009 | 19483662 |
| endocarditis due to neisseria bacilliformis in a patient with a bicuspid aortic valve. | we report a case of endocarditis due to the rod-shaped neisseria species neisseria bacilliformis. the phenotypic characterization of this recently characterized bacteria is difficult, and the identification requires the sequencing of the 16s rrna gene. the resolution of the disease was complete after appropriate antibiotic therapy, and surgery was not required. | 2009 | 19386832 |
| structural characterization of an oligosaccharide made by neisseria sicca. | neisseria sicca 4320 expresses two carbohydrate-containing components with sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities that resemble those of lipooligosaccharide and lipopolysaccharide. using matrix-assisted laser desorption ionization--time of flight and electrospray ionization mass spectrometry, we characterized a disaccharide carbohydrate repeating unit expressed by this strain. gas chromatography identified the sugars composing the unit as rhamnose and n-acetyl-d-glu ... | 2009 | 19270085 |
| [protease activity of microflora in the oral cavity of patients with periodontitis]. | microbial spectrum and non-specific as well as specific iga1 protease activity of isolated microorganisms were investigated in gingival liquid of patients with periodontitis. microorganisms from the gingival liqud of these patients belonged to conditional-pathogenic obligate and facultatively anaerobic bacteria. 24 strains of microorganisms have been identified. nonspecific proteolytic activity was found in the following microorganisms: actinomyces israelii, actinomyces naeslundii, aerococcus vi ... | 2008 | 19205430 |
| identification of meningococcal genes necessary for colonization of human upper airway tissue. | neisseria meningitidis is an exclusively human pathogen that has evolved primarily to colonize the nasopharynx rather than to cause systemic disease. colonization is the most frequent outcome following meningococcal infection and a prerequisite for invasive disease. the mechanism of colonization involves attachment of the organism to epithelial cells via bacterial type iv pili (tfp), but subsequent events during colonization remain largely unknown. we analyzed 576 n. meningitidis mutants for the ... | 2009 | 18936183 |
| identification of meningococcal genes necessary for colonization of human upper airway tissue. | neisseria meningitidis is an exclusively human pathogen that has evolved primarily to colonize the nasopharynx rather than to cause systemic disease. colonization is the most frequent outcome following meningococcal infection and a prerequisite for invasive disease. the mechanism of colonization involves attachment of the organism to epithelial cells via bacterial type iv pili (tfp), but subsequent events during colonization remain largely unknown. we analyzed 576 n. meningitidis mutants for the ... | 2009 | 18936183 |
| acute meningitis caused by neisseria sicca. | 1948 | 18911348 | |
| multicenter evaluation of the new vitek 2 neisseria-haemophilus identification card. | the new neisseria-haemophilus identification (nh) card for vitek 2 was compared with 16s rrna gene sequencing (16s) as the reference method for accurate identification of neisseria spp., haemophilus spp., and other fastidious gram-negative bacteria. testing was performed on the vitek 2 xl system with modified software at three clinical trial laboratories. reproducibility was determined with nine atcc quality control strains tested 20 times over a minimum of 10 days at all three sites. a challeng ... | 2008 | 18579712 |
| decontamination effects of low-temperature plasma generated by corona discharge. part ii: new insights. | the second part of our paper presents the results of experiments with the decontamination of surfaces by low-temperature plasma generated by corona discharge in air at atmospheric pressure. a simple device is described and the effects of the corona discharge on model microorganisms, viz. the yeast candida albicans, gram-negative bacteria escherichia coli, enterobacter aerogenes, neisseria sicca, stenotrophomonas maltophilia, gram-positive bacteria deinococcus radiodurans, enterococcus faecium, s ... | 2007 | 18225640 |
| the laboratory diagnosis of neisseria gonorrhoeae. | the present article describes the laboratory diagnosis of neisseria gonorrhoeae by culturing of the organism from different types of clinical specimens followed by confirmatory tests. the success of culture methods requires good quality collection and transport of clinical specimens. the present guide describes the media requirements and cultural conditions for n gonorrhoeae growth and the characteristics for a presumptive identification of n gonorrhoeae. confirmatory tests include biochemical t ... | 2005 | 18159523 |
| roles of alpha and beta carbonic anhydrases of helicobacter pylori in the urease-dependent response to acidity and in colonization of the murine gastric mucosa. | carbon dioxide occupies a central position in the physiology of helicobacter pylori owing to its capnophilic nature, the large amounts of carbon dioxide produced by urease-mediated urea hydrolysis, and the constant bicarbonate supply in the stomach. carbonic anhydrases (ca) catalyze the interconversion of carbon dioxide and bicarbonate and are involved in functions such as co(2) transport or trapping and ph homeostasis. h. pylori encodes a periplasmic alpha-ca (alpha-ca-hp) and a cytoplasmic bet ... | 2008 | 18025096 |
| roles of alpha and beta carbonic anhydrases of helicobacter pylori in the urease-dependent response to acidity and in colonization of the murine gastric mucosa. | carbon dioxide occupies a central position in the physiology of helicobacter pylori owing to its capnophilic nature, the large amounts of carbon dioxide produced by urease-mediated urea hydrolysis, and the constant bicarbonate supply in the stomach. carbonic anhydrases (ca) catalyze the interconversion of carbon dioxide and bicarbonate and are involved in functions such as co(2) transport or trapping and ph homeostasis. h. pylori encodes a periplasmic alpha-ca (alpha-ca-hp) and a cytoplasmic bet ... | 2008 | 18025096 |
| liver abscess due to neisseria sicca after repeated transcatheter arterial embolization. | neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). a case of a diabetic man with infected biloma due to n. sicca after repeated transcatheter arterial embolization for hepatocellular carcinoma is reported. the patient was successfully treated with intravenous cefotaxime and metronidazole. the biochemical profile and 16s rrna gene partial sequencing results of the ... | 2007 | 17965360 |
| neisseria sicca meningitis following intracranial hemorrhage and ventriculostomy tube placement. | a normal component of the flora of the oropharynx, neisseria sicca was first isolated in 1906 and has since been reported as a rare cause of various human infections including endocarditis, pneumonia, sinusitis, sepsis, and urethritis. we report the case of a 44-year-old african-american female with a history of hypertension who presented with complaints of right frontal headache, nausea, photophobia, and vomiting. a computed tomography scan of the patient's brain showed a large subarachnoid hem ... | 2007 | 17904282 |
| physiological ecology of stenoxybacter acetivorans, an obligate microaerophile in termite guts. | stenoxybacter acetivorans is a newly described, obligately microaerophilic beta-proteobacterium that is abundant in the acetate-rich hindgut of reticulitermes. here we tested the hypotheses that cells are located in the hypoxic, peripheral region of reticulitermes flavipes hindguts and use acetate to fuel their o(2)-consuming respiratory activity in situ. physical fractionation of r. flavipes guts, followed by limited-cycle pcr with s. acetivorans-specific 16s rrna gene primers, indicated that c ... | 2007 | 17827335 |
| the pathogenicity of neisseria sicca. | 1932 | 17817576 | |
| microbiological evaluation of the new vitek 2 neisseria-haemophilus identification card. | vitek 2 is an automated identification system for diverse bacterial and fungal species. a new card (the neisseria-haemophilus [nh] card) for the identification of neisseria spp., haemophilus spp., and other fastidious gram-negative or gram-variable microorganisms has been developed, but its performance in a routine clinical laboratory has not yet been evaluated. in this study, a total of 188 bacterial strains belonging to the genera actinobacillus, campylobacter, capnocytophaga, cardiobacterium, ... | 2007 | 17728469 |
| severe infective endocarditis due to neisseria sicca: case report and review of literature. | we report a case of infective endocarditis (ie) caused by an unusual micro-organism in a previously healthy young man. our patient presented with meningo-encephalitis and embolic signs due to ie caused by neisseria sicca. risk factors for ie due to neisseria sicca, such as intravenous drug use and pre-existing heart disease were absent. the patient recovered well after mitral valve surgery and antimicrobial therapy. ie due to neisseria spp. is associated with embolic manifestations and valve des ... | 2006 | 17240741 |
| characteristics of the m2000 automated sample preparation and multiplex real-time pcr system for detection of chlamydia trachomatis and neisseria gonorrhoeae. | we evaluated a new real-time pcr-based prototype assay for the detection of chlamydia trachomatis and neisseria gonorrhoeae developed by abbott molecular inc. this assay is designed to be performed on an abbott m2000 real-time instrument system, which consists of an m2000sp instrument for sample preparation and an m2000rt instrument for real-time pcr amplification and detection. the limit of detection of this prototype assay was determined to be 20 copies of target dna for both c. trachomatis an ... | 2007 | 17202273 |
| rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time pcr assay and melt curve analysis. | metallo-beta-lactamase enzymes (mbetal) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time pcr assay followed by a melt curve step for rapid detection and identification of genes encoding mbetal-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding imp and vim types, spm-1, gim-1, and sim-1 were downloaded from genbank, and primers were designed t ... | 2007 | 17093019 |
| rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time pcr assay and melt curve analysis. | metallo-beta-lactamase enzymes (mbetal) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time pcr assay followed by a melt curve step for rapid detection and identification of genes encoding mbetal-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding imp and vim types, spm-1, gim-1, and sim-1 were downloaded from genbank, and primers were designed t ... | 2007 | 17093019 |
| a fast real-time polymerase chain reaction method for sensitive and specific detection of the neisseria gonorrhoeae pora pseudogene. | ever since the advent of molecular methods, the diagnostics of neisseria gonorrhoeae has been troubled by false negative and false positive results compared with culture. commensal neisseria species and neisseria meningitidis are closely related to n. gonorrhoeae and may cross-react when using molecular tests comprising too-low specificity. we have devised a real-time polymerase chain reaction (pcr), including an internal amplification control, that targets the n. gonorrhoeae pora pseudogene. dn ... | 2006 | 17065426 |
| the majority of genes in the pathogenic neisseria species are present in non-pathogenic neisseria lactamica, including those designated as 'virulence genes'. | neisseria meningitidis causes the life-threatening diseases meningococcal meningitis and meningococcal septicemia. neisseria gonorrhoeae is closely related to the meningococcus, but is the cause of the very different infection, gonorrhea. a number of genes have been implicated in the virulence of these related yet distinct pathogens, but the genes that define and differentiate the species and their behaviours have not been established. further, a related species, neisseria lactamica is not assoc ... | 2006 | 16734888 |
| molecular characterization of subject-specific oral microflora during initial colonization of enamel. | the initial microbial colonization of tooth surfaces is a repeatable and selective process, with certain bacterial species predominating in the nascent biofilm. characterization of the initial microflora is the first step in understanding interactions among community members that shape ensuing biofilm development. using molecular methods and a retrievable enamel chip model, we characterized the microbial diversity of early dental biofilms in three subjects. a total of 531 16s rrna gene sequences ... | 2006 | 16597990 |
| the biotin requirements of neisseria sicca. | 1946 | 16561136 | |
| variations in gene organization and dna uptake signal sequence in the folp region between commensal and pathogenic neisseria species. | horizontal gene transfer is an important source of genetic variation among neisseria species and has contributed to the spread of resistance to penicillin and sulfonamide drugs in the pathogen neisseria meningitidis. sulfonamide resistance in neisseria meningitidis is mediated by altered chromosomal folp genes. at least some folp alleles conferring resistance have been horizontally acquired from other species, presumably from commensal neisseriae. in this work, the dna sequence surrounding folp ... | 2006 | 16503987 |
| nucleic acid amplification testing for neisseria gonorrhoeae: an ongoing challenge. | nucleic acid amplification tests (naats) for the detection of neisseria gonorrhoeae became available in the early 1990s. although offering several advantages over traditional detection methods, n. gonorrhoeae naats do have some limitations. these include cost, risk of carryover contamination, inhibition, and inability to provide antibiotic resistance data. in addition, there are sequence-related limitations that are unique to n. gonorrhoeae naats. in particular, false-positive results are a majo ... | 2006 | 16436629 |
| specific and sensitive detection of neisseria gonorrhoeae in clinical specimens by real-time pcr. | early diagnosis of neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. we report the development of a specific and sensitive taqman assay for the detection of n. gonorrhoeae in clinical samples. the target sequence is a 76-bp fragment of the 5' untranslated region of the opa genes that encode opacity proteins. a panel of 448 well-defined n. gonorrhoeae isolates was used to evaluate and optimize the assay. the method employs two minor-groove binding prob ... | 2005 | 16272500 |
| contribution of systematic serological testing in diagnosis of infective endocarditis. | despite progress with diagnostic criteria, the type and timing of laboratory tests used to diagnose infective endocarditis (ie) have not been standardized. this is especially true with serological testing. patients with suspected ie were evaluated by a standard diagnostic protocol. this protocol mandated an evaluation of the patients according to the modified duke criteria and used a battery of laboratory investigations, including three sets of blood cultures and systematic serological testing f ... | 2005 | 16207989 |
| a case of polymicrobial endocarditis caused by anaerobic organisms in an injection drug user. | endocarditis is a serious complication of injection drug use most commonly caused by staphylococcus aureus. we report a case of tricuspid valve polymicrobial bacterial endocarditis in an injection drug user from 3 oral anaerobes: actinomyces odontolytica, veillonella species, and prevotella melaninogenica. the patient was believed to have acquired these organisms from his habit of licking the needle in order to gauge the strength of the cocaine prior to injection. the patient was successfully tr ... | 2005 | 16191149 |
| mode of action on deacetylation of acetylated methyl glycoside by cellulose acetate esterase from neisseria sicca sb. | the regioselective deacetylation of purified cellulose acetate esterase from neisseria sicca sb was investigated on methyl 2,3,4,6-tetra-o-acetyl-beta-d-glucopyranoside and 2,3,4,6-tetra-o-acetyl-beta-d-galactopyranoside. the substrates were used as model compounds of cellulose acetate in order to estimate the mechanism for deacetylation of cellulose acetate by the enzyme. the enzyme rapidly deacetylated at position c-3 of methyl 2,3,4,6-tetra-o-acetyl-beta-d-glucopyranoside to accumulate 2,4,6- ... | 2005 | 16041133 |
| evaluation of pcr-based assay for diagnosis of spotted fever group rickettsiosis in human serum samples. | a nested pcr assay was developed for the detection of spotted fever group (sfg) rickettsiae in serum samples. the assay was based on specific primers derived from the rickettsial outer membrane protein b gene (rompb) of rickettsia conorii. an sfg rickettsia-specific signal is obtained from r. akari, r. japonica, r. sibirica, and r. conorii. other bacterial species tested did not generate any signal, attesting to the specificity of the assay. as few as seven copies of the rompb gene of r. conorii ... | 2005 | 15939751 |
| evaluation of conventional and real-time pcr assays using two targets for confirmation of results of the cobas amplicor chlamydia trachomatis/neisseria gonorrhoeae test for detection of neisseria gonorrhoeae in clinical samples. | two conventional pcr-enzyme immunoassays (pcr-eias) and two real-time pcr assays (lightcycler system; roche diagnostics) were evaluated as confirmation assays with cppb and 16s rrna genes as targets. of 765 male and female genitourinary and nasopharyngeal specimens positive for neisseria gonorrhoeae in the cobas amplicor chlamydia trachomatis/neisseria gonorrhoeae pcr test (roche diagnostics), 229 (30%) were confirmed positive; 13 of these (5.7%) were lacking the cppb gene. of the 534 samples (7 ... | 2005 | 15872247 |
| a novel pora-based real-time pcr for detection of meningococcal carriage. | real-time pcr based on the capsule transfer gene (ctra) is a significant aid in the diagnosis of meningococcal infection but fails to detect a high proportion (60 %) of non-groupable strains associated with nasopharyngeal carriage. this study aimed to design a novel real-time (taqman) pcr that would detect more strains of meningococci and be suitable for large-scale carriage studies. primer and probe sequences were based on the meningococcal pora gene and designed specifically to exclude the hig ... | 2005 | 15824424 |
| mycobacterium fortuitum infection after a brown bear bite. | 2005 | 15695734 | |
| pcr detection of bacteria on cardiac valves of patients with treated bacterial endocarditis. | we used broad-range pcr amplification and sequencing to detect and identify bacterial dna in 156 valves of patients treated for infective endocarditis (ie). bacterial dna was found more frequently in patients who underwent valve replacement while on antibiotic treatment for ie (60%) than in patients who had completed antibiotic treatment for ie (37%; p = 0.02). we found specific bacterial dna in valves removed from 11 of 30 patients who had completed antibiotic treatment for ie. six had no histo ... | 2005 | 15634966 |
| [anticoagulation with bemiparina after intracerebral hemorrhage as complication of bacterial endocarditis on metallic prosthetic valve]. | we present the case of a patient with an intracerebral hemorrhage as complication of an infectious endocarditis (ei) produced by neisseria sicca on a prosthetic mitral valve. the patient was anticoagulated previously with acenocumarol as prophylaxis of thrombosis of the prosthetic valve. he was diagnosed as having ie and later he presented neurological symptoms as consequence of several intracerebral hemorrhagic foci. we decided to continue the anticoagulation with sodium heparin followed of bem ... | 2004 | 15538906 |
| molecular detection of coxiella burnetii in the sera of patients with q fever endocarditis or vascular infection. | in the absence of a specific diagnosis based on serology, chronic q fever is inevitably fatal. however, diagnosis is often delayed because the test is not widely available. to shorten the diagnostic delay, we adapted a nested-pcr assay with serum as a template and the lightcycler as a thermal cycler, termed lcn-pcr. we retrospectively and prospectively applied this method to samples from 48 patients diagnosed with q fever endocarditis or vascular infection and to samples from 100 controls with e ... | 2004 | 15528674 |
| effect of amoxicillin use on oral microbiota in young children. | dental plaque samples from 40 children were screened for the presence of bacteria resistant to amoxicillin. fifteen children had used amoxicillin and 25 had not used any antibiotic in the 3 months prior to sample collection. all (100%) of the children harbored amoxicillin-resistant oral bacteria. the median percentage of the total cultivable oral microbiota resistant to amoxicillin was 2.4% (range, 0.1 to 14.3%) in children without amoxicillin use and 10.9% (range, 0.8 to 97.3%) in children with ... | 2004 | 15273096 |
| abstracts of the 27th annual meeting of the society of general internal medicine. chicago, illinois, usa, 12-15 may, 2004. | 2004 | 15107043 | |
| vaccine potential of the neisseria meningitidis 2086 lipoprotein. | a novel antigen that induces cross-reactive bactericidal antibodies against a number of neisseria meningitidis strains is described. this antigen, a approximately 28-kda lipoprotein called lp2086, was first observed within a complex mixture of soluble outer membrane proteins (somps) following a series of fractionation, protein purification, and proteomics steps. approximately 95 different neisserial isolates tested positive by western blotting and pcr screening methods for the presence of the pr ... | 2004 | 15039331 |
| use of real-time pcr to resolve slide agglutination discrepancies in serogroup identification of neisseria meningitidis. | neisseria meningitidis is a leading cause of bacterial meningitis and septicemia in children and young adults in the united states. rapid and reliable identification of n. meningitidis serogroups is crucial for judicious and expedient response to cases of meningococcal disease, including decisions about vaccination campaigns. from 1997 to 2002, 1,298 n. meningitidis isolates, collected in the united states through the active bacterial core surveillance (abcs), were tested by slide agglutination ... | 2004 | 14715772 |
| infective discitis with neisseria sicca/subflava in a previously healthy adult. | a case report of neisseria sicca/subflava discitis in a healthy elderly female. | 2003 | 14504620 |
| [humoral reactivity of rabbits vaccinated with neisseria pharyngis var. flava, neisseria catarrhalis & neisseria sicca]. | 1957 | 13504596 | |
| [research on various bacteria of the mouth & pharynx: neisseria catarrhalis & neisseria sicca]. | 1957 | 13504595 | |
| [experimental thyropathy by inoculation of neisseria sicca into the apex of lingual v in rat]. | 1954 | 13230262 | |
| detection of helicobacter pylori in gastric mucosa of patients with gastroduodenal diseases by pcr-restriction analysis using the rna polymerase gene (rpob). | a novel pcr restriction analysis method using the rna polymerase beta-subunit- coding gene (rpob) was employed to both detect and identify helicobacter pylori in biopsy specimens and culture isolates. the rpob dnas (458 bp) were specifically amplified by pcr with the helicobacter-specific primers (hf and hr). based on the determined rpob sequences of the culture isolates, an h. pylori-specific restriction site, tru9i, was found. h. pylori can be identified by observing two discernible dna fragme ... | 2003 | 12843100 |
| role of endo-1,4-beta-glucanases from neisseria sicca sb in synergistic degradation of cellulose acetate. | an enzyme hydrolyzing beta-1,4 bonds in cellulose acetate was purified 10.5-fold to electrophoretic homogeneity from a culture supernatant of neisseria sicca sb, which assimilate cellulose acetate as the sole carbon and energy source. the enzyme was an endo-1,4-beta-glucanase, to judge from the substrate specificity and hydrolysis products of cellooligosaccharides, we named it endo-1,4-beta-glucanase i (eg i). its molecular mass was 50 kda, 9 kda larger than eg ii from this strain, and its isoel ... | 2003 | 12728982 |
| the moraxella catarrhalis immunoglobulin d-binding protein mid has conserved sequences and is regulated by a mechanism corresponding to phase variation. | the prevalence of the moraxella catarrhalis immunoglobulin d (igd)-binding outer membrane protein mid and its gene was determined in 91 clinical isolates and in 7 culture collection strains. eighty-four percent of the clinical moraxella strains expressed mid-dependent igd binding. the mid gene was detected in all strains as revealed by homology of the signal peptide sequence and a conserved area in the 3' end of the gene. when mid proteins from five different strains were compared, an identity o ... | 2003 | 12644500 |
| evaluation of the specificities of five dna amplification methods for the detection of neisseria gonorrhoeae. | the intragenus specificities of five molecular diagnostic methods for neisseria gonorrhoeae were determined. three assays were considered suboptimal. molecular detection of n. gonorrhoeae from sites where other neisseria spp. commonly occur or from any site in low-prevalence settings should be confirmed by a test targeting a different genetic locus. | 2003 | 12574295 |
| identification of haemophilus influenzae serotypes by standard slide agglutination serotyping and pcr-based capsule typing. | to resolve discrepancies in slide agglutination serotyping (sast) results from state health departments and the centers for disease control and prevention (cdc), we characterized 141 of 751 invasive haemophilus influenzae isolates that were identified in the united states from january 1998 to december 1999 through an active, laboratory-based, surveillance program coordinated by the cdc. we found discrepancies between the results of sast performed at state health departments and those of pcr caps ... | 2003 | 12517878 |
| innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. | the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ... | 2002 | 12438343 |
| mosaic-like structure of penicillin-binding protein 2 gene (pena) in clinical isolates of neisseria gonorrhoeae with reduced susceptibility to cefixime. | neisseria gonorrhoeae strains with reduced susceptibility to cefixime (mics, 0.25 to 0.5 micro g/ml) were isolated from male urethritis patients in tokyo, japan, in 2000 and 2001. the resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, n. gonorrhoeae atcc 19424, by transformation of the penicillin-binding protein 2 gene (pena) that had been amplified by pcr from a strain with reduced susceptibility to cefixime (mic, 0.5 micro g/ml). the sequences o ... | 2002 | 12435671 |
| sulphonamide resistant commensal neisseria with alterations in the dihydropteroate synthase can be isolated from carriers not exposed to sulphonamides. | development of sulphonamide resistance in neisseria meningitidis has been suggested to involve horizontal dna-transfer from a commensal neisseria species. in this study, we isolated commensal neisseria from throat specimens and examined the isolates with respect to sulphonamide resistance. | 2002 | 12435277 |
| assessment of the nasopharyngeal bacterial flora of rhesus macaques: moraxella, neisseria, haemophilus, and other genera. | the nasopharyngeal bacterial flora of healthy rhesus macaques was surveyed for the presence of neisseria and haemophilus species, as well as moraxella catarrhalis. m. catarrhalis was found both in healthy rhesus macaques and in possibly immunocompromised rhesus macaques. several haemophilus spp. that are part of the normal nasopharyngeal bacterial flora of humans were found in many animals; these haemophilus species included h. parahaemolyticus, h. segnis, and h. parainfluenzae. while haemophilu ... | 2002 | 12409426 |
| the class a macrophage scavenger receptor is a major pattern recognition receptor for neisseria meningitidis which is independent of lipopolysaccharide and not required for secretory responses. | macrophages (mphi) play a key role in the pathogenesis of invasive meningococcal infections. the roles of two pattern recognition molecules, the mphi scavenger receptor (sr-a) and toll-like receptor 4 (tlr-4), have been investigated using bone marrow culture-derived mphi (bmmphi). surprisingly, a comparison of bmmphi from wild-type and sr-a knockout (sr-a(-/-)) mice showed that nonopsonic phagocytosis of meningococci was mediated almost exclusively via sr-a. previous studies have demonstrated on ... | 2002 | 12228258 |
| purification and characterization of an endo-1,4-beta-glucanase from neisseria sicca sb that hydrolyzes beta-1,4 linkages in cellulose acetate. | an enzyme catalyzing hydrolysis of beta-1,4 bonds in cellulose acetate was purified 18.3-fold to electrophoretic homogeneity from a culture supernatant of neisseria sicca sb, which can assimilate cellulose acetate as the sole carbon and energy source. the molecular mass of the enzyme was 41 kda and the isoelectric point was 4.8. the ph and temperature optima of the enzyme were 6.0-7.0 and 60 degrees c. the enzyme catalyzed hydrolysis of water-soluble cellulose acetate (degree of substitution, 0. ... | 2002 | 12005042 |
| detection of meningococcal carriage by culture and pcr of throat swabs and mouth gargles. | the standard method for detecting meningococcal carriage is culture of throat swabs on selective media, but the levels of carriage determined depend heavily on the skills of the individuals taking the swab and interpreting the cultures. this study aimed to determine the most sensitive detection method for meningococcal carriage. throat swabs and saline mouth gargles, obtained from 89 university students, were processed in parallel by conventional culture and taqman ctra pcr. carriage of meningoc ... | 2002 | 11773095 |
| comparative biology of incq and incq-like plasmids. | plasmids belonging to escherichia coli incompatibility group q are relatively small (approximately 5 to 15 kb) and able to replicate in a remarkably broad range of bacterial hosts. these include gram-positive bacteria such as brevibacterium and mycobacterium and gram-negative bacteria such as agrobacterium, desulfovibrio, and cyanobacteria. these plasmids are mobilized by several self-transmissible plasmids into an even more diverse range of organisms including yeasts, plants, and animal cells. ... | 2001 | 11729261 |
| acid stress upregulated outer membrane proteins in clinical isolates of neisseria gonorrhoeae, but not most commensal neisseria. | human immune serum recognition of outer membrane components from commensal and pathogenic neisseria cultured under neutral and acidic conditions was investigated. acid stress caused no detectable alterations in lipooligosaccharide migration and (or) staining, in outer membrane protein profiles, or in immune serum recognition of outer membrane components from neisseria mucosa or neisseria sicca. there was also no difference in the lipoologosaccharide electrophoretic pattern of acid- and neutral-g ... | 2001 | 11683469 |
| [utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients]. | the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ... | 2001 | 11594009 |
| enhancing the specificity of the cobas amplicor ct/ng test for neisseria gonorrhoeae by retesting specimens with equivocal results. | the cobas amplicor ct/ng test for neisseria gonorrhoeae cross-reacts with certain strains of nonpathogenic neisseria species. in some strains, the target sequence is identical to that of n. gonorrhoeae, whereas other strains have a small number of mismatches within the regions recognized by the primers or probe used in the cobas amplicor ng test. these cross-reactive strains are occasionally present in urogenital specimens, causing false-positive results in the cobas amplicor ng test. analysis o ... | 2001 | 11526134 |
| new tests for syphilis: rational design of a pcr method for detection of treponema pallidum in clinical specimens using unique regions of the dna polymerase i gene. | a sensitive and specific pcr method to detect treponema pallidum in clinical specimens was developed. pcr primers were designed based on two unique features of the dna polymerase i gene (pola). the first distinctive characteristic is that the region codes for a high cysteine content and has low homology with similar regions of dna polymerase i gene from known microorganisms. the second unique feature is the presence of four insertions in the gene. pcr tests using primers designed on the basis th ... | 2001 | 11326018 |
| diagnostics of neisseriaceae and moraxellaceae by ribosomal dna sequencing: ribosomal differentiation of medical microorganisms. | fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. however, in the case of some fastidious gram-negative bacterial species, classical phenotype identification based on either metabolic, enzymatic, or serological methods is difficult, time-consuming, and/or inadequate. 16s or 23s ribosomal dna (rdna) bacterial sequencing will most often result in accurate speciation of isolates. therefore, the objective of this study was to find a hypervariable ... | 2001 | 11230407 |
| analysis of lipooligosaccharide biosynthesis in the neisseriaceae. | neisserial lipooligosaccharide (los) contains three oligosaccharide chains, termed the alpha, beta, and gamma chains. we used southern hybridization experiments on dna isolated from various neisseria spp. to determine if strains considered to be nonpathogenic possessed dna sequences homologous with genes involved in the biosynthesis of these oligosaccharide chains. the presence or absence of specific genes was compared to the los profiles expressed by each strain, as characterized by their mobil ... | 2001 | 11208792 |
| selective sensitization of bacteria to peroxide damage associated with fluoride inhibition of catalase and pseudocatalase. | fluoride and sulfide are known inhibitors of heme catalases in acid environments. staphylococcus aureus h cells were found to be sensitized by fluoride or sulfide to h2o2 killing at acid ph values in the range of 3.5 to 4.0, and catalase activity was reduced concomitantly. in contrast, fluoride had little effect on h2o2 killing of streptococcus mutans gs-5, which has fluoride-insensitive peroxidase activity, but still is more sensitive to h2o2 than is s. aureus in the absence of fluoride. fluori ... | 2001 | 11169136 |
| endocarditis due to rare and fastidious bacteria. | the etiologic diagnosis of infective endocarditis is easily made in the presence of continuous bacteremia with gram-positive cocci. however, the blood culture may contain a bacterium rarely associated with endocarditis, such as lactobacillus spp., klebsiella spp., or nontoxigenic corynebacterium, salmonella, gemella, campylobacter, aeromonas, yersinia, nocardia, pasteurella, listeria, or erysipelothrix spp., that requires further investigation to establish the relationship with endocarditis, or ... | 2001 | 11148009 |
| a case of continuous ambulatory peritoneal dialysis peritonitis with an uncommon organism and an atypical clinical course. | this report describes a 46-year-old patient who experienced an atypical course of peritonitis while undergoing continuous ambulatory peritoneal dialysis (capd). the first sign of peritonitis was progressive impairment of ultrafiltration with increasing fluid absorption. the patient came to the center after 5 days with leg edemas and 645 leukocytes/microl in the first dialysate outflow. on the same day, the dialysate cell count decreased to 208/microl. during the following days, ultrafiltration f ... | 2001 | 11136199 |
| neisseria sicca meningitis in a woman with nascent pernicious anemia. | 2000 | 11032571 | |
| prokaryotic carbonic anhydrases. | carbonic anhydrases catalyze the reversible hydration of co(2) [co(2)+h(2)oright harpoon over left harpoon hco(3)(-)+h(+)]. since the discovery of this zinc (zn) metalloenzyme in erythrocytes over 65 years ago, carbonic anhydrase has not only been found in virtually all mammalian tissues but is also abundant in plants and green unicellular algae. the enzyme is important to many eukaryotic physiological processes such as respiration, co(2) transport and photosynthesis. although ubiquitous in high ... | 2000 | 10978542 |
| gram-positive bacteria are potent inducers of monocytic interleukin-12 (il-12) while gram-negative bacteria preferentially stimulate il-10 production. | interleukin-10 (il-10) and il-12 are two cytokines secreted by monocytes/macrophages in response to bacterial products which have largely opposite effects on the immune system. il-12 activates cytotoxicity and gamma interferon (ifn-gamma) secretion by t cells and nk cells, whereas il-10 inhibits these functions. in the present study, the capacities of gram-positive and gram-negative bacteria to induce il-10 and il-12 were compared. monocytes from blood donors were stimulated with uv-killed bacte ... | 2000 | 10816515 |
| detection of legionella species in respiratory specimens using pcr with sequencing confirmation. | legionella spp. are a common cause of community-acquired respiratory tract infections and an occasional cause of nosocomial pneumonia. a pcr method for the detection of legionellae in respiratory samples was evaluated and was compared to culture. the procedure can be performed in 6 to 8 h with a commercially available dna extraction kit (qiagen, valencia, calif.) and by pcr with gel detection. pcr is performed with primers previously determined to amplify a 386-bp product within the 16s rrna gen ... | 2000 | 10790085 |
| characterization of the ftsz cell division gene of neisseria gonorrhoeae: expression in escherichia coli and n. gonorrhoeae. | we cloned the cell division gene ftsz of the gram-negative coccus neisseria gonorrhoeae (ng) strain ch811, characterized it genetically and phenotypically, and studied its localization in n. gonorrhoeae and escherichia coli (ec). the 1,179-bp orf of ftsz(ng) encodes a protein with a predicted molecular mass of 41.5 kda. protein sequence alignments indicate that ftsz(ng) is similar to other ftsz proteins and contains the conserved gtp binding motif. ftsz homologues were identified in several n. g ... | 2000 | 10648099 |
| carbonic anhydrase is an ancient enzyme widespread in prokaryotes. | carbonic anhydrases catalyze the reversible hydration of co(2) and are ubiquitous in highly evolved eukaryotes. the recent identification of a third class of carbonic anhydrase (gamma class) in a methanoarchaeon and our present finding that the beta class also extends into thermophilic species from the archaea domain led us to initiate a systematic search for these enzymes in metabolically and phylogenetically diverse prokaryotes. here we show that carbonic anhydrase is widespread in the archaea ... | 1999 | 10611359 |
| purification and characterization of an esterase involved in cellulose acetate degradation by neisseria sicca sb. | an esterase catalyzing the hydrolysis of acetyl ester moieties in cellulose acetate was purified 1,110-fold to electrophoretic homogeneity from the culture supernatant of neisseria sicca sb, which can assimilate cellulose acetate as the sole carbon and energy source. the purified enzyme was a monomeric protein with a molecular mass of 40 kda and the isoelectric point was 5.3. the ph and temperature optima of the enzyme were 8.0-8.5 and 45 degrees c. the enzyme catalyzed the hydrolysis of acetyl ... | 1999 | 10586499 |
| structural and evolutionary inference from molecular variation in neisseria porins. | the porin proteins of the pathogenic neisseria species, neisseria gonorrhoeae and neisseria meningitidis, are important as serotyping antigens, putative vaccine components, and for their proposed role in the intracellular colonization of humans. a three-dimensional structural homology model for neisseria porins was generated from escherichia coli porin structures and n. meningitidis pora and porb sequences. the neisseria sequences were readily assembled into the 16-strand beta-barrel fold charac ... | 1999 | 10225902 |
| gram type-specific broad-range pcr amplification for rapid detection of 62 pathogenic bacteria. | broad-range pcr has proven to be useful for the detection of bacteria. a set of broad-range pcr primers directed against conserved regions in the 16s rrna gene was designed to specifically amplify either gram-positive or gram-negative bacteria. the gram type-specific broad-range pcr correctly classified all 62 pathogenic species tested. | 1999 | 9889245 |
| evaluation of amplicor neisseria gonorrhoeae pcr using cppb nested pcr and 16s rrna pcr. | certain strains of neisseria subflava and neisseria cinerea are known to produce false-positive results with the amplicor neisseria gonorrhoeae pcr (roche diagnostic systems, branchburg, n.j.). the analytical sensitivity and analytical specificity of three pcr tests were assessed with 3 geographically diverse n. gonorrhoeae strains and 30 non-n. gonorrhoeae neisseria spp. the sensitivities of the in-house nested cppb gene and the 16s rrna pcr methods were greater than that of the amplicor n. gon ... | 1999 | 9889224 |
| differentiation between mycobacterium tuberculosis and mycobacterium avium by amplification of the 16s-23s ribosomal dna spacer. | differentiation between mycobacterium tuberculosis and m. avium is helpful for the treatment of disseminated mycobacterial infection in aids patients. this can traditionally be done by time-consuming biochemical tests or with accuprobe. previously, pcr restriction enzyme analysis (pcr-rea) of the 16s-23s rrna gene spacer was shown to be able to identify a limited number of strains of mycobacterium. in this study the method was improved by using more specific primers and was tested with 50 clinic ... | 1998 | 9705362 |
| the regulated outer membrane protein omp21 from comamonas acidovorans is identified as a member of a new family of eight-stranded beta-sheet proteins by its sequence and properties. | omp21, a minor outer membrane protein of the soil bacterium comamonas acidovorans, was purified from a spontaneous mutant lacking a surface layer and long-chain lipopolysaccharide. omp21 synthesis is enhanced by oxygen depletion, and the protein has a variable electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis due to its heat-modifiable behavior. the structural gene omp21 encodes a precursor of 204 amino acids with a putative signal peptide of 21 amino acids. m ... | 1998 | 9683466 |
| septic arthritis caused by neisseria sicca. | 1998 | 9558202 | |
| immunoreactivity of five monoclonal antibodies against the 37-kilodalton common cell wall protein (psaa) of streptococcus pneumoniae. | five monoclonal antibodies (mabs) were produced against the streptococcus pneumoniae pneumococcal surface adhesin a (psaa) 37-kda common cell wall protein. these antibodies were used in a dot immunoblot and western blot study of clinical isolates of s. pneumoniae to detect the presence of the protein. by both assays, the mabs reacted with clinical isolates representing the 23 type-specific serotypes present in the licensed pneumococcal polysaccharide vaccine. western blot analysis confirmed the ... | 1998 | 9521144 |
| ciprofloxacin versus cefuroxime axetil in the treatment of adult patients with acute bacterial sinusitis. | this study compared the use and efficacy of ciprofloxacin to cefuroxime axetil for adult patients with acute bacterial sinusitis. | 1998 | 9511113 |
| bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds. | this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ... | 1998 | 9501433 |