Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| skin-derived c-terminal filaggrin-2 fragments are pseudomonas aeruginosa-directed antimicrobials targeting bacterial replication. | soil- and waterborne bacteria such as pseudomonas aeruginosa are constantly challenging body surfaces. since infections of healthy skin are unexpectedly rare, we hypothesized that the outermost epidermis, the stratum corneum, and sweat glands directly control the growth of p. aeruginosa by surface-provided antimicrobials. due to its high abundance in the upper epidermis and eccrine sweat glands, filaggrin-2 (flg2), a water-insoluble 248 kda s100 fused-type protein, might possess these innate eff ... | 2015 | 26371476 |
| preparation of dental resins resistant to enzymatic and hydrolytic degradation in oral environments. | the short average service life of traditional dental composite restorative materials and increasing occurrence of secondary caries adjacent to composite restorations and sealants are necessitating the development of new, longer lasting compositions. novel monomers and their polymers, reinforcing fillers, and adhesive components are needed. the goal of this research is to develop resin systems for use in restorations, sealants, and other dental services that are superior in properties and enduran ... | 2015 | 26358180 |
| live-streaming: time-lapse video evidence of novel streamer formation mechanism and varying viscosity. | time-lapse videos of growing biofilms were analyzed using a background subtraction method, which removed camouflaging effects from the heterogeneous field of view to reveal evidence of streamer formation from optically dense biofilm segments. in addition, quantitative measurements of biofilm velocity and optical density, combined with mathematical modeling, demonstrated that streamer formation occurred from mature, high-viscosity biofilms. we propose a streamer formation mechanism by sudden part ... | 2015 | 26339304 |
| brkautodisplay: functional display of multiple exogenous proteins on the surface of escherichia coli by using brka autotransporter. | bacterial surface display technique enables the exogenous proteins or polypeptides displayed on the bacterial surface, while maintaining their relatively independent spatial structures and biological activities. the technique makes recombinant bacteria possess the expectant functions, subsequently, directly used for many applications. many proteins could be used to achieve bacterial surface display, among them, autotransporter, a member of the type v secretion system of gram-negative bacteria, h ... | 2015 | 26337099 |
| metabolic degradation of 1,4-dichloronaphthalene by pseudomonas sp. hy. | there is increasing concern regarding the adverse health effects of polychlorinated naphthalenes (pcns). the metabolic degradation of 1,4-dichloronaphthalene (1,4-dcn) as a model pcn, was studied using a strain of pseudomonas sp. hy. the metabolites were analyzed by gas chromatography-mass spectrometry (gc-ms). a series of metabolites including dihydroxy-dichloro-naphthalene, epoxy-dichlorinated naphthalene, dichlorinated naphthol, and dichlorinated salicylic acid were identified. the time-conce ... | 2015 | 26308037 |
| overcoming differences: the catalytic mechanism of metallo-β-lactamases. | metallo-β-lactamases are the latest resistance mechanism of pathogenic and opportunistic bacteria against carbapenems, considered as last resort drugs. the worldwide spread of genes coding for these enzymes, together with the lack of a clinically useful inhibitor, have raised a sign of alarm. inhibitor design has been mostly impeded by the structural diversity of these enzymes. here we provide a critical review of mechanistic studies of the three known subclasses of metallo-β-lactamases, analyze ... | 2015 | 26297824 |
| biosurfactant production by bacillus salmalaya for lubricating oil solubilization and biodegradation. | this study investigated the capability of a biosurfactant produced by a novel strain of bacillus salmalaya to enhance the biodegradation rates and bioavailability of organic contaminants. the biosurfactant produced by cultured strain 139si showed high physicochemical properties and surface activity in the selected medium. the biosurfactant exhibited a high emulsification index and a positive result in the drop collapse test, with the results demonstrating the wetting activity of the biosurfactan ... | 2015 | 26295402 |
| multifarious activities of cellulose degrading bacteria from koala (phascolarctos cinereus) faeces. | cellulose degrading bacteria from koala faeces were isolated using caboxymethylcellulose-congo red agar, screened in vitro for different hydrolytic enzyme activities and phylogenetically characterized using molecular tools. bacillus sp. and pseudomonas sp. were the most prominent bacteria from koala faeces. the isolates demonstrated good xylanase, amylase, lipase, protease, tannase and lignin peroxidase activities apart from endoglucanase activity. furthermore many isolates grew in the presence ... | 2015 | 26290743 |
| treatment of uncomplicated symptomatic urinary tract infections: resistance patterns and misuse of antibiotics. | uncomplicated but symptomatic urinary tract infections (utis) are a common problem seen in practice. the study was undertaken to assess the most common pathogens responsible for uncomplicated symptomatic utis and the antimicrobial resistance pattern in a hospital in bangalore. the study also explores the issue of antibiotic usage for these patients. | 2015 | 26288784 |
| biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium. | three bacterial isolates identified as alcanivorax borkumensis sk2, rhodococcus erythropolis hs4 and pseudomonas stutzeri sdm, based on 16s rrna gene sequences, were isolated from crude oil enrichments of natural seawater. single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (alcanivorax: pseudomonas, 1:1), (alcanivorax: rhodococcus, 1:1), (pseudomonas: rhodococcus, 1:1), and (alcanivorax: pseudomonas: rhodococcus, 1:1 ... | 2015 | 26273252 |
| fermentative hydrogen production from agroindustrial lignocellulosic substrates. | to achieve economically competitive biological hydrogen production, it is crucial to consider inexpensive materials such as lignocellulosic substrate residues derived from agroindustrial activities. it is possible to use (1) lignocellulosic materials without any type of pretreatment, (2) lignocellulosic materials after a pretreatment step, and (3) lignocellulosic materials hydrolysates originating from a pretreatment step followed by enzymatic hydrolysis. according to the current literature data ... | 2015 | 26273246 |
| bioaugmentation of mesorhizobium cicer, pseudomonas spp. and piriformospora indica for sustainable chickpea production. | chickpea establishes symbiotic association with mesorhizobium to fulfill its nitrogen (n) requirement. integrating chickpea rhizosphere with potential native mesorhizobia and other plant growth promoting microorganisms can contribute multiple benefits to plants. the present investigation was undertaken to study interactions among piriformospora indica (pi) with potential plant growth promoting rhizobacteria (pgpr) viz. pseudomonas argentinensis (lpgpr1), pseudomonas sp. (lpgpr2) along with natio ... | 2015 | 26261403 |
| unearthing the genomes of plant-beneficial pseudomonas model strains wcs358, wcs374 and wcs417. | plant growth-promoting rhizobacteria (pgpr) can protect plants against pathogenic microbes through a diversity of mechanisms including competition for nutrients, production of antibiotics, and stimulation of the host immune system, a phenomenon called induced systemic resistance (isr). in the past 30 years, the pseudomonas spp. pgpr strains wcs358, wcs374 and wcs417 of the willie commelin scholten (wcs) collection have been studied in detail in pioneering papers on the molecular basis of pgpr-me ... | 2015 | 26198432 |
| encapsulating peritoneal sclerosis as a late complication of peritoneal dialysis. | encapsulating peritoneal sclerosis (eps) is a rare cause of intestinal obstruction which is characterized by fibrotic encapsulation of the bowel. although its pathogenesis is still not clear, many etiological factors have been stated. | 2015 | 26150908 |
| classes of organic molecules targeted by a methanogenic microbial consortium grown on sedimentary rocks of various maturities. | organic-rich shales are populated by methanogenic consortia that are able to degrade the fossilized organic matter into methane gas. to identify the organic fraction effectively degraded, we have sequentially depleted two types of organic-rich sedimentary rocks, shale, and coal, at two different maturities, by successive solvent extractions to remove the most soluble fractions (maltenes and asphaltenes) and isolate kerogen. we show the ability of the consortia to produce methane from all rock sa ... | 2015 | 26136731 |
| identification of an endophytic antifungal bacterial strain isolated from the rubber tree and its application in the biological control of banana fusarium wilt. | banana fusarium wilt (also known as panama disease) is one of the most disastrous plant diseases. effective control methods are still under exploring. the endophytic bacterial strain itbb b5-1 was isolated from the rubber tree, and identified as serratia marcescens by morphological, biochemical, and phylogenetic analyses. this strain exhibited a high potential for biological control against the banana fusarium disease. visual agar plate assay showed that itbb b5-1 restricted the mycelial growth ... | 2015 | 26133557 |
| analysis of fungal ball rhinosinusitis by culturing fungal clumps under endoscopic surgery. | this study was designed to investigate the clinical microbiology of fungal ball (fb) rhinosinusitis by culturing fungal clumps collected under endoscopic surgery. | 2015 | 26131186 |
| a facile method for controlling the reaction equilibrium of sphingolipid ceramide n-deacylase for lyso-glycosphingolipid production. | lyso-glycosphingolipids (lyso-gsls), the n-deacylated forms of glycosphingolipids (gsls), are important synthetic intermediates for the preparation of gsl analogs. although lyso-gsls can be produced by hydrolyzing natural gsls using sphingolipid ceramide n-deacylase (scdase), the yield for this reaction is usually low because scdase also catalyzes the reverse reaction, ultimately establishing an equilibrium between hydrolysis and synthesis. in the present study, we developed an efficient method ... | 2015 | 26130766 |
| abstracts, 9th german allergy congress, wiesbaden, 2-4 october 2014. | 2014 | 26120532 | |
| microbial population dynamics in response to pectobacterium atrosepticum infection in potato tubers. | endophytes are microbes and fungi that live inside plant tissues without damaging the host. herein we examine the dynamic changes in the endophytic bacterial community in potato (solanum tuberosum) tuber in response to pathogenic infection by pectobacterium atrosepticum, which causes soft rot in numerous economically important crops. we quantified community changes using both cultivation and next-generation sequencing of the 16s rrna gene and found that, despite observing significant variability ... | 2015 | 26118792 |
| seasonal variation in denitrification and dissimilatory nitrate reduction to ammonia process rates and corresponding key functional genes along an estuarine nitrate gradient. | this research investigated spatial-temporal variation in benthic bacterial community structure, rates of denitrification and dissimilatory nitrate reduction to ammonium (dnra) processes and abundances of corresponding genes and transcripts at three sites-the estuary-head, mid-estuary and the estuary mouth (em) along the nitrate gradient of the colne estuary over an annual cycle. denitrification rates declined down the estuary, while dnra rates were higher at the estuary head and middle than the ... | 2015 | 26082763 |
| efflux pump-deficient mutants as a platform to search for microbes that produce antibiotics. | pseudomonas putida dot-t1e-18 is a strain deficient in the major antibiotic efflux pump (ttgabc) that exhibits an overall increased susceptibility to a wide range of drugs when compared with the wild-type strain. we used this strain as a platform to search for microbes able to produce antibiotics that inhibit growth. a collection of 2400 isolates from soil, sediments and water was generated and a drop assay developed to identify, via growth inhibition halos, strains that prevent the growth of do ... | 2015 | 26059350 |
| lack of negative effects on syrian hamsters and mongolian gerbils housed in the same secondary enclosure. | in cases where different species might be housed in the same room or secondary enclosure, the guide for the care and use of laboratory animals recommends that the animals should be behaviorally compatible and have the same health status. syrian hamsters and mongolian gerbils, both desert-dwelling rodents, appear to be reasonable candidates for such a combination. this study was undertaken to evaluate whether housing hamsters and gerbils in the same secondary enclosure is an acceptable practice. ... | 2015 | 26045450 |
| the potential impact of coinfection on antimicrobial chemotherapy and drug resistance. | across a range of pathogens, resistance to chemotherapy is a growing problem in both public health and animal health. despite the ubiquity of coinfection, and its potential effects on within-host biology, the role played by coinfecting pathogens on the evolution of resistance and efficacy of antimicrobial chemotherapy is rarely considered. in this review, we provide an overview of the mechanisms of interaction of coinfecting pathogens, ranging from immune modulation and resource modulation, to d ... | 2015 | 26028590 |
| interactions of non-natural halogenated substrates with d-specific dehalogenase (dehd) mutants using in silico studies. | the d-2-haloacid dehalogenase of d-specific dehalogenase (dehd) from rhizobium sp. rc1 catalyses the hydrolytic dehalogenation of d-haloalkanoic acids, inverting the substrate-product configuration and thereby forming the corresponding l-hydroxyalkanoic acids. our investigations were focused on dehd mutants: r134a and y135a. we examined the possible interactions between these mutants with haloalkanoic acids and characterized the key catalytic residues in the wild-type dehalogenase, to design deh ... | 2014 | 26019583 |
| transcriptional modulation of intestinal innate defense/inflammation genes by preterm infant microbiota in a humanized gnotobiotic mouse model. | it is known that postnatal functional maturation of the small intestine is facilitated by microbial colonization of the gut. preterm infants exhibit defects in gut maturation, weak innate immunity against intestinal infection and increased susceptibility to inflammatory disorders, all of which may be related to the inappropriate microbial colonization of their immature intestines. the earliest microbes to colonize the preterm infant gut encounter a naïve, immature intestine. thus this earliest m ... | 2015 | 25928420 |
| the role of ala198 in the stability and coenzyme specificity of bacterial formate dehydrogenases. | it has been shown by an x-ray structural analysis that the amino acid residues ala198, which are located in the coenzyme-binding domain of nad(+)-dependent formate dehydrogenases (ec 1.2.1.2., fdh) from bacteria pseudomonas sp.101 and moraxella sp. c-1 (psefdh and morfdh, respectively), have non-optimal values of the angles ψ and φ. these residues were replaced with gly by site-directed mutagenesis. the mutants psefdh a198g and morfdh a198g were expressed in e.coli cells and obtained in active a ... | 2015 | 25927002 |
| plant-microbe cross-talk in the rhizosphere: insight and biotechnological potential. | rhizosphere, the interface between soil and plant roots, is a chemically complex environment which supports the development and growth of diverse microbial communities. the composition of the rhizosphere microbiome is dynamic and controlled by multiple biotic and abiotic factors that include environmental parameters, physiochemical properties of the soil, biological activities of the plants and chemical signals from the plants and bacteria which inhabit the soil adherent to root-system. recent a ... | 2015 | 25926899 |
| the toluene o-xylene monooxygenase enzymatic activity for the biosynthesis of aromatic antioxidants. | monocyclic phenols and catechols are important antioxidant compounds for the food and pharmaceutic industries; their production through biotransformation of low-added value starting compounds is of major biotechnological interest. the toluene o-xylene monooxygenase (tomo) from pseudomonas sp. ox1 is a bacterial multicomponent monooxygenase (bmm) that is able to hydroxylate a wide array of aromatic compounds and has already proven to be a versatile biochemical tool to produce mono- and dihydroxyl ... | 2015 | 25915063 |
| a redox regulatory system critical for mycobacterial survival in macrophages and biofilm development. | survival of m. tuberculosis in host macrophages requires the eukaryotic-type protein kinase g, pkng, but the underlying mechanism has remained unknown. here, we show that pkng is an integral component of a novel redox homeostatic system, rhocs, which includes the ribosomal protein l13 and renu, a nudix hydrolase encoded by a gene adjacent to pkng. studies in m. smegmatis showed that pkng expression is uniquely induced by nadh, which plays a key role in metabolism and redox homeostasis. in vitro, ... | 2015 | 25884716 |
| the crystal structure of d-threonine aldolase from alcaligenes xylosoxidans provides insight into a metal ion assisted plp-dependent mechanism. | threonine aldolases catalyze the pyridoxal phosphate (plp) dependent cleavage of threonine into glycine and acetaldehyde and play a major role in the degradation of this amino acid. in nature, l- as well as d-specific enzymes have been identified, but the exact physiological function of d-threonine aldolases (dtas) is still largely unknown. both types of enantio-complementary enzymes have a considerable potential in biocatalysis for the stereospecific synthesis of various β-hydroxy amino acids, ... | 2015 | 25884707 |
| comparing two types of rabbit atg prior to reduced intensity conditioning allogeneic hematopoietic sct for hematologic malignancies. | different rabbit polyclonal antilymphocyte globulins (atgs) are used in allogeneic hematopoietic stem cell transplantation (allohsct) to prevent graft-versus-host disease (gvhd). we compared 2 different atgs in allohsct after reduced intensity conditioning (ric) for hematological malignancies. we reviewed 30 allohsct for hematologic malignancies performed between 2007 and 2010 with fludarabine and i.v. busulfan as conditioning regimen. patients alternatingly received thymoglobulin or atg-f. medi ... | 2015 | 25874131 |
| encapsulating peritoneal sclerosis - a rare and serious complication of peritoneal dialysis: case series. | encapsulating peritoneal sclerosis is a pathological entity mainly associated with peritoneal dialysis (pd). the clinical syndrome is characterized by various degrees of intestinal obstruction due to thickening, sclerosis and calcification of peritoneum resulting in the encapsulation and cocooning of the bowel. it is a rare but potentially devastating complication associated with a considerable morbidity and mortality. | 2014 | 25870687 |
| bacteria isolated from bats inhibit the growth of pseudogymnoascus destructans, the causative agent of white-nose syndrome. | emerging infectious diseases are a key threat to wildlife. several fungal skin pathogens have recently emerged and caused widespread mortality in several vertebrate groups, including amphibians, bats, rattlesnakes and humans. white-nose syndrome, caused by the fungal skin pathogen pseudogymnoascus destructans, threatens several hibernating bat species with extinction and there are few effective treatment strategies. the skin microbiome is increasingly understood to play a large role in determini ... | 2015 | 25853558 |
| experimental design approach to the optimization of pahs bioremediation from artificially contaminated soil: application of variables screening development. | the effectiveness of bioremediation systems for pah-contaminated soil may be constrained by physicochemical properties of contaminants and environmental factors. information on what is the most effective factor in bioremediation process is essential in the decision of what stimulations can be taken to assist the biodegradation efficacy. | 2015 | 25834738 |
| alginate lyase: review of major sources and classification, properties, structure-function analysis and applications. | alginate lyases catalyze the degradation of alginate, a complex copolymer of α-l-guluronate and its c5 epimer β-d-mannuronate. the enzymes have been isolated from various kinds of organisms with different substrate specificities, including algae, marine mollusks, marine and terrestrial bacteria, and some viruses and fungi. with the progress of structural biology, many kinds of alginate lyases of different polysaccharide lyases families have been characterized by obtaining crystal structures, and ... | 2015 | 25831216 |
| comparative analysis of wolbachia genomes reveals streamlining and divergence of minimalist two-component systems. | two-component regulatory systems are commonly used by bacteria to coordinate intracellular responses with environmental cues. these systems are composed of functional protein pairs consisting of a sensor histidine kinase and cognate response regulator. in contrast to the well-studied caulobacter crescentus system, which carries dozens of these pairs, the streamlined bacterial endosymbiont wolbachia pipientis encodes only two pairs: ccka/ctra and plec/pled. here, we used bioinformatic tools to co ... | 2015 | 25809075 |
| phytogenic compounds as alternatives to in-feed antibiotics: potentials and challenges in application. | this article summarizes current experimental knowledge on the efficacy, possible mechanisms and feasibility in the application of phytogenic products as feed additives for food-producing animals. phytogenic compounds comprise a wide range of plant-derived natural bioactive compounds and essential oils are a major group. numerous studies have demonstrated that phytogenic compounds have a variety of functions, including antimicrobial/antiviral, antioxidative and anti-inflammation effects and impro ... | 2015 | 25806623 |
| clinical outcomes and microbiological characteristics of severe pneumonia in cancer patients: a prospective cohort study. | pneumonia is the most frequent type of infection in cancer patients and a frequent cause of icu admission. the primary aims of this study were to describe the clinical and microbiological characteristics and outcomes in critically ill cancer patients with severe pneumonia. | 2015 | 25803690 |
| detection of metallo-beta lactamases among carbapenem-resistant pseudomonas aeruginosa. | carbapenems are important drugs used for the treatment of pseudomonas aeruginosa infections, however metallo-β-lactamases (mbl) are able to efficiently hydrolyze these classes of drugs. immediate detection of the mbl-producing p. aeruginosa is necessary in order to accurately treat infections caused by this organism. | 2014 | 25774271 |
| cultivable endophytic bacteria from leaf bases of agave tequilana and their role as plant growth promoters. | agave tequilana weber var. 'azul' is grown for the production of tequila, inulin and syrup. diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. in this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy agave tequilana plants. in plant tissue bacteria occurred at mean population densities of 3 million cfu/g of fresh plant tissue. three hundred endophytic strains were isolated and 16s rdna sequences grouped the bacteria into ... | 2014 | 25763038 |
| cultivable endophytic bacteria from leaf bases of agave tequilana and their role as plant growth promoters. | agave tequilana weber var. 'azul' is grown for the production of tequila, inulin and syrup. diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. in this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy agave tequilana plants. in plant tissue bacteria occurred at mean population densities of 3 million cfu/g of fresh plant tissue. three hundred endophytic strains were isolated and 16s rdna sequences grouped the bacteria into ... | 2014 | 25763038 |
| a bioinformatic survey of distribution, conservation, and probable functions of luxr solo regulators in bacteria. | luxr solo transcriptional regulators contain both an autoinducer binding domain (abd; n-terminal) and a dna binding helix-turn-helix domain (hth; c-terminal), but are not associated with a cognate n-acyl homoserine lactone (ahl) synthase coding gene in the same genome. although a few luxr solos have been characterized, their distributions as well as their role in bacterial signal perception and other processes are poorly understood. in this study we have carried out a systematic survey of distri ... | 2015 | 25759807 |
| soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. | salinity is one of the most brutal environmental factors limiting the productivity of crop plants because most of the crop plants are sensitive to salinity caused by high concentrations of salts in the soil, and the area of land affected by it is increasing day by day. for all important crops, average yields are only a fraction - somewhere between 20% and 50% of record yields; these losses are mostly due to drought and high soil salinity, environmental conditions which will worsen in many region ... | 2014 | 25737642 |
| soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. | salinity is one of the most brutal environmental factors limiting the productivity of crop plants because most of the crop plants are sensitive to salinity caused by high concentrations of salts in the soil, and the area of land affected by it is increasing day by day. for all important crops, average yields are only a fraction - somewhere between 20% and 50% of record yields; these losses are mostly due to drought and high soil salinity, environmental conditions which will worsen in many region ... | 2014 | 25737642 |
| the induction of ethylene response factor 3 (erf3) in potato as a result of co-inoculation with pseudomonas sp. r41805 and rhizophagus irregularis mucl 41833 - a possible role in plant defense. | colonization of plant rhizosphere/roots by beneficial microorganisms (e.g. plant growth promoting rhizobacteria - pgpr, arbuscular mycorrhizal fungi - amf) confers broad-spectrum resistance to virulent pathogens and is known as induced systemic resistance (isr) and mycorrhizal-induced resistance (mir). isr or mir, an indirect mechanism for biocontrol, involves complex signaling networks that are regulated by several plant hormones, the most important of which are salicylic acid (sa), jasmonic ac ... | 2015 | 25723847 |
| phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. | phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement. although abundant in soils, in both organic and inorganic forms, its availability is restricted as it occurs mostly in insoluble forms. the p content in average soil is about 0.05% (w/w) but only 0.1% of the total p is available to plant because of poor solubility and its fixation in soil (illmer and schinner, soil biol biochem 27:257-263, 1995). an adequate supply of p ... | 2013 | 25674415 |
| statistically optimized biotransformation protocol for continuous production of l-dopa using mucuna monosperma callus culture. | l-dopa (3,4-dihydroxyphenyl-l-alanine), a modified amino acid, is an expansively used drug for the parkinson's disease treatment. in the present study, optimization of nutritional parameters influencing l-dopa production was attempted using the response surface methodology (rsm) from mucuna monosperma callus. optimization of the four factors was carried out using the box-behnken design. the optimized levels of factors predicted by the model include tyrosine 0.894 g l(-1), ph 4.99, ascorbic acid ... | 2013 | 25674405 |
| identification of a histidine metal ligand in the arge-encoded n-acetyl-l-ornithine deacetylase from escherichia coli. | the h355a, h355k, h80a, and h80k mutant enzymes of the arge-encoded n-acetyl-l-ornithine deacetylase (arge) from escherichia coli were prepared, however, only the h355a enzyme was found to be soluble. kinetic analysis of the co(ii)-loaded h355a exhibited activity levels that were 380-fold less than co(ii)-loaded wt arge. electronic absorption spectra of co(ii)-loaded h355a-arge indicate that the bound co(ii) ion resides in a distorted, five-coordinate environment and isothermal titration calorim ... | 2013 | 25674394 |
| marine sediments microbes capable of electrode oxidation as a surrogate for lithotrophic insoluble substrate metabolism. | little is known about the importance and/or mechanisms of biological mineral oxidation in sediments, partially due to the difficulties associated with culturing mineral-oxidizing microbes. we demonstrate that electrochemical enrichment is a feasible approach for isolation of microbes capable of gaining electrons from insoluble minerals. to this end we constructed sediment microcosms and incubated electrodes at various controlled redox potentials. negative current production was observed in incub ... | 2014 | 25642220 |
| marine sediments microbes capable of electrode oxidation as a surrogate for lithotrophic insoluble substrate metabolism. | little is known about the importance and/or mechanisms of biological mineral oxidation in sediments, partially due to the difficulties associated with culturing mineral-oxidizing microbes. we demonstrate that electrochemical enrichment is a feasible approach for isolation of microbes capable of gaining electrons from insoluble minerals. to this end we constructed sediment microcosms and incubated electrodes at various controlled redox potentials. negative current production was observed in incub ... | 2014 | 25642220 |
| comparison of microbiomes from different niches of upper and lower airways in children and adolescents with cystic fibrosis. | changes in the airway microbiome may be important in the pathophysiology of chronic lung disease in patients with cystic fibrosis. however, little is known about the microbiome in early cystic fibrosis lung disease and the relationship between the microbiomes from different niches in the upper and lower airways. therefore, in this cross-sectional study, we examined the relationship between the microbiome in the upper (nose and throat) and lower (sputum) airways from children with cystic fibrosis ... | 2015 | 25629612 |
| nanomedicine and therapy of lung diseases. | the use of nanotechnology has significantly increased in different fields of science, including the development of drug delivery systems. currently, the most modern pharmaceutical nanocarriers, such as liposomes, micelles, nanoemulsions and polymeric nanoparticles, demonstrate extremely useful properties from the point of view of drug therapy. in this context, the development of nanocarriers for pulmonary application has been much debated by the scientific community in recent decades. although r ... | 2014 | 25628213 |
| neonatal conjunctivitis - a review. | ophthalmia neonatorum remains a significant cause of ocular morbidity, blindness and even death in underdeveloped countries. the organisms causing ophthalmia neonatorum are acquired mainly from the mother's birth canal during delivery and a small percentage of cases are acquired by other ways. chlamydia and neisseria are the most common pathogens responsible for the perinatal infection. fortunately in most cases, laboratory studies can identify the causative organism and unlike other form of con ... | 2008 | 25606121 |
| trapping and assembly of living colloids at water-water interfaces. | we study the assembly of inert and living colloids in a two-phase water-water system that provides an environment that can sustain bacteria, providing a new structure with rich potential to confine and structure microbial communities. the water-water system, formed via phase separation of a casein and xanthan mixture, forms a 3-d structure of coexisting casein-rich and xanthan-rich phases. fluorescent labelling and confocal microscopy reveal the attachment of these living colloids, including esc ... | 2015 | 25600991 |
| applying nightingale charts to evaluate the heterogeneity of biomedical waste in a hospital. | to evaluate the heterogeneity of biomedical waste (bw) using nightingale charts. | 2014 | 25591088 |
| novel assay to measure the plasmid mobilizing potential of mixed microbial communities. | mobilizable plasmids lack necessary genes for complete conjugation and are therefore non-self-transmissible. instead, they rely on the conjugation system of conjugal plasmids to be horizontally transferred to new recipients. while community permissiveness, the fraction of a mixed microbial community that can receive self-transmissible conjugal plasmids, has been studied, the intrinsic ability of a community to mobilize plasmids that lack conjugation systems is unexplored. here, we present a nove ... | 2014 | 25566238 |
| bacteria-bacteria interactions within the microbiota of the ancestral metazoan hydra contribute to fungal resistance. | epithelial surfaces of most animals are colonized by diverse microbial communities. although it is generally agreed that commensal bacteria can serve beneficial functions, the processes involved are poorly understood. here we report that in the basal metazoan hydra, ectodermal epithelial cells are covered with a multilayered glycocalyx that provides a habitat for a distinctive microbial community. removing this epithelial microbiota results in lethal infection by the filamentous fungus fusarium ... | 2014 | 25514534 |
| bacteria-bacteria interactions within the microbiota of the ancestral metazoan hydra contribute to fungal resistance. | epithelial surfaces of most animals are colonized by diverse microbial communities. although it is generally agreed that commensal bacteria can serve beneficial functions, the processes involved are poorly understood. here we report that in the basal metazoan hydra, ectodermal epithelial cells are covered with a multilayered glycocalyx that provides a habitat for a distinctive microbial community. removing this epithelial microbiota results in lethal infection by the filamentous fungus fusarium ... | 2014 | 25514534 |
| bioconversion of α-pinene by a novel cold-adapted fungus chrysosporium pannorum. | the psychrotrophic fungus chrysosporium pannorum a-1 is reported for the first time as a novel biocatalyst for o2-promoted oxidation of α-pinene. gc-ms analysis indicated that the main products of the reaction were compounds of a high commercial value, verbenol (1) and verbenone (2). exponentially growing cells (days 2-3) were about twice as active as cells in the late stationary phase in terms of the total concentration of products. the highest yields of 1 and 2 were obtained using three-day an ... | 2014 | 25487757 |
| bioconversion of α-pinene by a novel cold-adapted fungus chrysosporium pannorum. | the psychrotrophic fungus chrysosporium pannorum a-1 is reported for the first time as a novel biocatalyst for o2-promoted oxidation of α-pinene. gc-ms analysis indicated that the main products of the reaction were compounds of a high commercial value, verbenol (1) and verbenone (2). exponentially growing cells (days 2-3) were about twice as active as cells in the late stationary phase in terms of the total concentration of products. the highest yields of 1 and 2 were obtained using three-day an ... | 2014 | 25487757 |
| significance of serum antibodies against hsp 60 and hsp 70 for the diagnostic of infectious diseases. | heat shock proteins (hsp) represent important antigenic targets for the immune response, playing an important role in the pathology and infectious diseases control. the purpose of this work was to investigate the levels of hsp60 and hsp70 specific antibodies in the bloodstream of patients with different bacterial infections and cancer, in order to evaluate their potential role as diagnosis markers of different infectious diseases. detection of specific anti-hsp 60 and hsp 70 serum levels was per ... | 2014 | 25483863 |
| dissolving mechanism of strain p17 on insoluble phosphorus of yellow-brown soil. | strain p17 was a bacterial strain identified as bacillus megaterium isolated from ground accumulating phosphate rock powder. the fermentation broth of strain p17 and the yellow-brown soil from nanjing agricultural university garden were collected to conduct this study. the simulation of fixed insoluble phosphorous forms after applying calcium superphosphate into yellow-brown soil was performed in pots, while available p and total p of soil were extremely positive correlative with those of ground ... | 2014 | 25477929 |
| genomics-based exploration of virulence determinants and host-specific adaptations of pseudomonas syringae strains isolated from grasses. | the pseudomonas syringae species complex has recently been named the number one plant pathogen, due to its economic and environmental impacts, as well as for its role in scientific research. the bacterium has been repeatedly reported to cause outbreaks on bean, cucumber, stone fruit, kiwi and olive tree, as well as on other crop and non-crop plants. it also serves as a model organism for research on the type iii secretion system (t3ss) and plant-pathogen interactions. while most of the current w ... | 2014 | 25437611 |
| reactive oxygen species mediated bacterial biofilm inhibition via zinc oxide nanoparticles and their statistical determination. | the formation of bacterial biofilm is a major challenge in clinical applications. the main aim of this study is to describe the synthesis, characterization and biocidal potential of zinc oxide nanoparticles (nps) against bacterial strain pseudomonas aeruginosa. these nanoparticles were synthesized via soft chemical solution process in a very short time and their structural properties have been investigated in detail by using x-ray diffraction and transmission electron microscopy measurements. in ... | 2014 | 25402188 |
| silencing by h-ns potentiated the evolution of salmonella. | the bacterial h-ns protein silences expression from sequences with higher at-content than the host genome and is believed to buffer the fitness consequences associated with foreign gene acquisition. loss of h-ns results in severe growth defects in salmonella, but the underlying reasons were unclear. an experimental evolution approach was employed to determine which secondary mutations could compensate for the loss of h-ns in salmonella. six independently derived s. typhimurium hns mutant strains ... | 2014 | 25375226 |
| draft genome sequence analysis of a pseudomonas putida w15oct28 strain with antagonistic activity to gram-positive and pseudomonas sp. pathogens. | pseudomonas putida is a member of the fluorescent pseudomonads known to produce the yellow-green fluorescent pyoverdine siderophore. p. putida w15oct28, isolated from a stream in brussels, was found to produce compound(s) with antimicrobial activity against the opportunistic pathogens staphylococcus aureus, pseudomonas aeruginosa, and the plant pathogen pseudomonas syringae, an unusual characteristic for p. putida. the active compound production only occurred in media with low iron content and w ... | 2014 | 25369289 |
| study of the structure-function-stability relationships in yeast d-amino acid oxidase: hydrophobization of alpha-helices. | hydrophobization of alpha-helices is one of the general approaches used for improving the thermal stability of enzymes. a total of 11 serine residues located in alpha-helices have been found based on multiple alignments of the amino acid sequences of d-amino acid oxidases from different organisms and the analysis of the 3d-structure of d-amino acid oxidase from yeast trigonopsis variabilis (tvdaao, ec 1.4.3.3). as a result of further structural analysis, eight ser residues in 67, 77, 78, 105, 27 ... | 2014 | 25349716 |
| chromobacterium csp_p reduces malaria and dengue infection in vector mosquitoes and has entomopathogenic and in vitro anti-pathogen activities. | plasmodium and dengue virus, the causative agents of the two most devastating vector-borne diseases, malaria and dengue, are transmitted by the two most important mosquito vectors, anopheles gambiae and aedes aegypti, respectively. insect-bacteria associations have been shown to influence vector competence for human pathogens through multi-faceted actions that include the elicitation of the insect immune system, pathogen sequestration by microbes, and bacteria-produced anti-pathogenic factors. t ... | 2014 | 25340821 |
| bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality. | some previous studies have identified bacteria in semen as being a potential factor in male infertility. however, only few types of bacteria were taken into consideration while using pcr-based or culturing methods. here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. ninety-six semen samples collected were examined for bacterial communities, measuring seven cli ... | 2014 | 25340531 |
| prophylactic antibody treatment and intramuscular immunization reduce infectious human rhinovirus 16 load in the lower respiratory tract of challenged cotton rats. | human rhinoviruses (hrv) represent the single most important etiological agents of the common cold and are the most frequent cause of acute respiratory infections in humans. currently the performance of available animal models for immunization studies using hrv challenge is very limited. the cotton rat (sigmodon hispidus) is a well-recognized model for the study of human respiratory viral infections. in this work we show that, without requiring any genetic modification of either the host or the ... | 2014 | 25328560 |
| membrane bioprocesses for pharmaceutical micropollutant removal from waters. | the purpose of this review work is to give an overview of the research reported on bioprocesses for the treatment of domestic or industrial wastewaters (ww) containing pharmaceuticals. conventional ww treatment technologies are not efficient enough to completely remove all pharmaceuticals from water. indeed, these compounds are becoming an actual public health problem, because they are more and more present in underground and even in potable waters. different types of bioprocesses are described ... | 2014 | 25295629 |
| the non-canonical hydroxylase structure of yfcm reveals a metal ion-coordination motif required for ef-p hydroxylation. | ef-p is a bacterial trna-mimic protein, which accelerates the ribosome-catalyzed polymerization of poly-prolines. in escherichia coli, ef-p is post-translationally modified on a conserved lysine residue. the post-translational modification is performed in a two-step reaction involving the addition of a β-lysine moiety and the subsequent hydroxylation, catalyzed by poxa and yfcm, respectively. the β-lysine moiety was previously shown to enhance the rate of poly-proline synthesis, but the role of ... | 2014 | 25274739 |
| growth kinetics, effect of carbon substrate in biosynthesis of mcl-pha by pseudomonas putida bet001. | growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-pha) in pseudomonas putida bet001 isolated from palm oil mill effluent was studied. models with substrate inhibition terms described well the kinetics of its growth. selected fatty acids (c8:0 to c18:1) and ammonium were used as carbon and nitrogen sources during growth and pha biosynthesis, resulting in pha accumulation of about 50 to 69% (w/w) and pha yields ranging from 10.12 g l(-1) to 15.45 g l(-1), respecti ... | 2014 | 25242925 |
| isolation and characterization of atrazine mineralizing bacillus subtilis strain hb-6. | atrazine is a widely used herbicide with great environmental concern due to its high potential to contaminate soil and waters. an atrazine-degrading bacterial strain hb-6 was isolated from industrial wastewater and the 16s rrna gene sequencing identified hb-6 as a bacillus subtilis. pcr assays indicated that hb-6 contained atrazine-degrading genes trzn, atzb and atzc. the strain hb-6 was capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaved the s-t ... | 2014 | 25238246 |
| rhamnolipid biosurfactants-past, present, and future scenario of global market. | 2014 | 25228898 | |
| outcomes of patients with healthcare-associated pneumonia: worse disease or sicker patients? | healthcare-associated pneumonia (hcap) is an entity distinct from community-acquired pneumonia (cap). hcap has a higher case-fatality rate, due either to hcap organisms or to the health status of hcap patients. the contribution of hcap criteria to case-fatality rate is unknown. | 2014 | 25222889 |
| synonymous codon bias and functional constraint on gc3-related dna backbone dynamics in the prokaryotic nucleoid. | while mrna stability has been demonstrated to control rates of translation, generating both global and local synonymous codon biases in many unicellular organisms, this explanation cannot adequately explain why codon bias strongly tracks neighboring intergene gc content; suggesting that structural dynamics of dna might also influence codon choice. because minor groove width is highly governed by 3-base periodicity in gc, the existence of triplet-based codons might imply a functional role for the ... | 2014 | 25200075 |
| p. aeruginosa sgnh hydrolase-like proteins algj and algx have similar topology but separate and distinct roles in alginate acetylation. | the o-acetylation of polysaccharides is a common modification used by pathogenic organisms to protect against external forces. pseudomonas aeruginosa secretes the anionic, o-acetylated exopolysaccharide alginate during chronic infection in the lungs of cystic fibrosis patients to form the major constituent of a protective biofilm matrix. four proteins have been implicated in the o-acetylation of alginate, algijf and algx. to probe the biological function of algj, we determined its structure to 1 ... | 2014 | 25165982 |
| characterization of the medium- and long-chain n-alkanes degrading pseudomonas aeruginosa strain sjtd-1 and its alkane hydroxylase genes. | a gram-negative aliphatic hydrocarbon-degrading bacterium sjtd-1 isolated from oil-contaminated soil was identified as pseudomonas aeruginosa by comparative analyses of the 16s rrna sequence, phenotype, and physiological features. sjtd-1 could efficiently mineralize medium- and long-chain n-alkanes (c12-c30) as its sole carbon source within seven days, showing the most optimal growth on n-hexadecane, followed by n-octadecane, and n-eicosane. in 36 h, 500 mg/l of tetradecane, hexadecane, and octa ... | 2014 | 25165808 |
| quality parameters, fatty acid profiling and estimation of umbelliferone in grahaṇimihira tailam: an ayurvedic oil preparation. | grahaṇimihira tailam is an unexplored ayurvedic oil preparation which consists of 34 ingredients. the efficacy of this traditional ayurvedic medicine is undisputable. proper clinical standardization of this formulation will go a long way in securing greater recognition for it. the main objective of this study was to develop standardization parameters for the formulation in a multidisciplinary way. | 2013 | 25161324 |
| does netosis contribute to the bacterial pathoadaptation in cystic fibrosis? | significant advances in our understanding of neutrophil biology were made in the past several years. the exciting discovery that neutrophils deploy neutrophil extracellular traps (nets) to catch pathogens paved the way for a series of additional studies to define the molecular mechanisms of net generation and the biological significance of netosis in acute and chronic pathologic conditions. this review highlights the latest knowledge regarding net structures, deployment, and function, with an em ... | 2014 | 25157250 |
| degradation of toluene by ortho cleavage enzymes in burkholderia fungorum flu100. | burkholderia fungorum flu100 simultaneously oxidized any mixture of toluene, benzene and mono-halogen benzenes to (3-substituted) catechols with a selectivity of nearly 100%. further metabolism occurred via enzymes of ortho cleavage pathways with complete mineralization. during the transformation of 3-methylcatechol, 4-carboxymethyl-2-methylbut-2-en-4-olide (2-methyl-2-enelactone, 2-ml) accumulated transiently, being further mineralized only after a lag phase of 2 h in case of cells pre-grown on ... | 2014 | 25130674 |
| degradation of toluene by ortho cleavage enzymes in burkholderia fungorum flu100. | burkholderia fungorum flu100 simultaneously oxidized any mixture of toluene, benzene and mono-halogen benzenes to (3-substituted) catechols with a selectivity of nearly 100%. further metabolism occurred via enzymes of ortho cleavage pathways with complete mineralization. during the transformation of 3-methylcatechol, 4-carboxymethyl-2-methylbut-2-en-4-olide (2-methyl-2-enelactone, 2-ml) accumulated transiently, being further mineralized only after a lag phase of 2 h in case of cells pre-grown on ... | 2014 | 25130674 |
| antibiotic susceptibilities of bacteria isolated within the oral flora of florida blacktip sharks: guidance for empiric antibiotic therapy. | sharks possess a variety of pathogenic bacteria in their oral cavity that may potentially be transferred into humans during a bite. the aim of the presented study focused on the identification of the bacteria present in the mouths of live blacktip sharks, carcharhinus limbatus, and the extent that these bacteria possess multi-drug resistance. swabs were taken from the oral cavity of nineteen live blacktip sharks, which were subsequently released. the average fork length was 146 cm (±11), suggest ... | 2014 | 25110948 |
| identification of a novel nidovirus in an outbreak of fatal respiratory disease in ball pythons (python regius). | respiratory infections are important causes of morbidity and mortality in reptiles; however, the causative agents are only infrequently identified. | 2014 | 25106433 |
| newly identified pathogens associated with periodontitis: a systematic review. | there is substantial evidence supporting the role of certain oral bacteria species in the onset and progression of periodontitis. nevertheless, results of independent-culture diagnostic methods introduced about a decade ago have pointed to the existence of new periodontal pathogens. however, the data of these studies have not been evaluated together, which may generate some misunderstanding on the actual role of these microorganisms in the etiology of periodontitis. the aim of this systematic re ... | 2014 | 25074492 |
| structural and functional analysis show that the escherichia coli uncharacterized protein yjcs is likely an alkylsulfatase. | sodium dodecyl sulfate (sds) is a widely used anionic surfactant in industry and research settings, and is known to have a detrimental effect to the environment. the pathway of sds degradation by bacteria is initiated by an alkylsulfatase and the oxidized product, 1-dodecanoic acid, subsequently enters into the β-oxidation pathway and is used as a carbon source. in this work, we solved the crystal structure of escherichia coli uncharacterized protein yjcs and identified that it belongs to the ty ... | 2014 | 25066955 |
| efficient asymmetric synthesis of 1-cyano-tetrahydroisoquinolines from lipase dual activity and opposite enantioselectivities in α-aminonitrile resolution. | dual promiscuous racemization/amidation activities of lipases leading to efficient dynamic kinetic resolution protocols of racemic α-aminonitrile compounds are described. α-amidonitrile products of high enantiomeric purity could be formed in high yields. several lipases from different sources were shown to exhibit the dual catalytic activities, where opposite enantioselectivities could be recorded for certain substrates. | 2014 | 25055970 |
| two structurally different dienelactone hydrolases (tfdei and tfdeii) from cupriavidus necator jmp134 plasmid pjp4 catalyse cis- and trans-dienelactones with similar efficiency. | in this study, dienelactone hydrolases (tfdei and tfdeii) located on plasmid pjp4 of cupriavidus necator jmp134 were cloned, purified, characterized and three dimensional structures were predicted. tfdei and tfdeii genes were cloned into pet21b vector and expressed in e. coli bl21(de3). the enzymes were purified by applying ultra-membrane filtration, anion-exchange qff and gel-filtration columns. the enzyme activity was determined by using cis-dienelactone. the three-dimensional structure of enz ... | 2014 | 25054964 |
| spatial heterogeneity of bacteria: evidence from hot composts by culture-independent analysis. | the phylogenetic diversity of the bacteria in hot composting samples collected from three spatial locations was investigated by molecular tools in order to determine the influence of gradient effect on bacterial communities during the thermophilic phase of composting swine manure with rice straw. total microbial dna was extracted and bacterial near full-length 16s rrna genes were subsequently amplified, cloned, restriction fragment length polymorphism-screened and sequenced. the superstratum sam ... | 2012 | 25049662 |
| sepsis in intensive care unit patients with traumatic brain injury: factors associated with higher mortality. | patients with traumatic brain injury are particularly susceptible to sepsis, which may exacerbate the systemic inflammatory response and lead to organ dysfunction. the influence of clinical variables on the mortality of intensive care unit patients with traumatic brain injury and sepsis was investigated. | 2014 | 25028949 |
| rate of positive urine culture and double-j catheters colonization on the basis of microorganism dna analysis. | the aim of the trial was to estimate the relationship between colonization of the double-j catheter, and the microorganisms cultured from urine. | 2014 | 24982789 |
| identification and characterization of the spiruchostatin biosynthetic gene cluster enable yield improvement by overexpressing a transcriptional activator. | spiruchostatins a and b are members of the fk228-family of natural products with potent histone deacetylase inhibitory activities and antineoplastic activities. however, their production in the wild-type strain of pseudomonas sp. q71576 is low. to improve the yield, the spiruchostatin biosynthetic gene cluster (spi) was first identified by rapid genome sequencing and characterized by genetic mutations. this spi gene cluster encodes a hybrid biosynthetic pathway similar to that encoded by the fk2 ... | 2014 | 24973954 |
| comparison of bacterial community structure and dynamics during the thermophilic composting of different types of solid wastes: anaerobic digestion residue, pig manure and chicken manure. | this study investigated the impact of composting substrate types on the bacterial community structure and dynamics during composting processes. to this end, pig manure (pm), chicken manure (cm), a mixture of pm and cm (pm + cm), and a mixture of pm, cm and anaerobic digestion residue (adr) (pm + cm + adr) were selected for thermophilic composting. the bacterial community structure and dynamics during the composting process were detected and analysed by polymerase chain reaction-denaturing gradie ... | 2014 | 24963997 |
| computational design of a ph stable enzyme: understanding molecular mechanism of penicillin acylase's adaptation to alkaline conditions. | protein stability provides advantageous development of novel properties and can be crucial in affording tolerance to mutations that introduce functionally preferential phenotypes. consequently, understanding the determining factors for protein stability is important for the study of structure-function relationship and design of novel protein functions. thermal stability has been extensively studied in connection with practical application of biocatalysts. however, little work has been done to ex ... | 2014 | 24959852 |
| a novel peptidoglycan binding protein crucial for pbp1a-mediated cell wall biogenesis in vibrio cholerae. | the bacterial cell wall, which is comprised of a mesh of polysaccharide strands crosslinked via peptide bridges (peptidoglycan, pg), is critical for maintenance of cell shape and survival. pg assembly is mediated by a variety of penicillin binding proteins (pbp) whose fundamental activities have been characterized in great detail; however, there is limited knowledge of the factors that modulate their activities in different environments or growth phases. in vibrio cholerae, the cause of cholera, ... | 2014 | 24945690 |
| microbial community structures and dynamics in the o3/bac drinking water treatment process. | effectiveness of drinking water treatment, in particular pathogen control during the water treatment process, is always a major public health concern. in this investigation, the application of pcr-dgge technology to the analysis of microbial community structures and dynamics in the drinking water treatment process revealed several dominant microbial populations including: α-proteobacteria, β-proteobacteria, γ-proteobacteria, bacteroidetes, actinobacteria firmicutes and cyanobacteria. α-proteobac ... | 2014 | 24937529 |
| melioidosis presenting as lymphadenitis: a case report. | melioidosis is an infection caused by the facultative intracellular gram-negative bacterium; burkholderia pseudomallei. it gives rise to protean clinical manifestations and has a varied prognosis. although it was rare in sri lanka increasing numbers of cases are being reported with high morbidity and mortality. here we report a case of melioidosis presenting with lymphadenitis which was diagnosed early and treated promptly with a good outcome. | 2014 | 24927768 |
| from community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic bacteroidetes generalists. | the microbiota of multi-pond solar salterns around the world has been analyzed using a variety of culture-dependent and molecular techniques. however, studies addressing the dynamic nature of these systems are very scarce. here we have characterized the temporal variation during 1 year of the microbiota of five ponds with increasing salinity (from 18% to >40%), by means of card-fish and dgge. microbial community structure was statistically correlated with several environmental parameters, includ ... | 2014 | 24926861 |