Publications

fine structure of fruiting bodies of stigmatella aurantiaca (myxobacterales).	the fruiting body of stigmatella aurantiaca consists of a thick stalk supporting a number of individual cysts. the stalk is made of discontinuous tubules, of dimensions known for vegetative cells, which are oriented parallel to the longitudinal axis of the stalk. the red-brown cysts contain numerous, randomly oriented myxospores which are surrounded by thick, fibrous capsules. their cell walls are wavy or ruffled and exhibit fewer budlike infoldings than reported for myxospores induced in liquid ...	1969	5370282
studies on stigmatella brunnea.		1969	4894284
carotenoid pigments of stigmatella aurantiaca (myxobacterales).i. the minor carotenoids.		1969	4315734
fatty acids of myxococcus xanthus.	fatty acids were extracted from saponified vegetative cells and myxospores of myxococcus xanthus and examined as the methyl esters by gas-liquid chromatography. the acids consisted mainly of c(14) to c(17) species. branched acids predominated, and iso-pentadecanoic acid constituted half or more of the mixture. the other leading component (11-28%) was found to be 11-n-hexadecenoic acid. among the unsaturated acids were two diunsaturated ones, an n-hexadecadienoic acid and an iso-heptadecadienoic ...	1973	4197903
distribution of multicopy single-stranded dna among myxobacteria and related species.	multicopy single-stranded dna (msdna) is a short single-stranded linear dna originally discovered in myxococcus xanthus and subsequently found in stigmatella aurantiaca. it exists at an estimated 500 to 700 copies per chromosome (t. yee, t. furuichi, s. inouye, and m. inouye, cell 38:203-209, 1984). we found msdna in other myxobacteria, including myxococcus coralloides, cystobacter violaceus, cystobacter ferrugineus (cbfe17), nannocystis exedens, and nine independently isolated strains of m. xan ...	1985	3932332
morphogenesis of stigmatella aurantiaca fruiting bodies.	scanning electron micrographs of intermediate stages of fruiting body formation in the myxobacterium stigmatella aurantiaca suggest that fruiting body formation can be divided into several stages distinguishable on the basis of the motile behavior of the cells. aggregates formed at sites where cells glide as groups in circles or spirals. thus, each aggregate was surrounded by a wide band of cells. several streams of cells were pointed toward and connected to the wide band of cells at the base of ...	1985	3926747
energy-dependent cell cohesion in myxobacteria.	cohesion in the myxobacterium stigmatella aurantiaca was characterized. two classes of cohesion were revealed, termed class a and class b. class a cohesion is a characteristic of vegetative cells grown in tryptone or casitone (difco laboratories, detroit, mich.), whereas class b cohesion requires the addition of calcium ion for induction. class a cohesion occurs in the presence of any cation and is temperature independent. class b cohesion requires the presence of a cation in the calcium group a ...	1985	3917996
the aurachins, new quinoline antibiotics from myxobacteria: production, physico-chemical and biological properties.	the aurachins, new quinoline alkaloids, were extracted with acetone from the biomass of the myxobacterium, stigmatella aurantiaca strain sg a15 and purified by column chromatography. the four described aurachins a, b, c and d, were inhibitory for gram-positive bacteria and a few yeasts and molds. they blocked nadh oxidation in beef heart submitochondrial particles.	1987	3106289
transfer of incp plasmids into stigmatella aurantiaca leading to insertional mutants affected in spore development.	derivatives of the broad-host-range plasmid rp4, containing the wild-type or modified transposon tn5 were transferred by conjugation to various stigmatella aurantiaca isolates. the transposons and in some cases fragments of the plasmid as well were integrated into the chromosome. thus, insertional mutants have been obtained affected in spore formation in liquid culture.	1988	2853291
calcium requirement for gliding motility in myxobacteria.	the ability to glide on a solid surface was inducible by calcium ion in stigmatella aurantiaca. the induction of motility but not motility itself was prevented by chloramphenicol and erythromycin. calcium ion was also required for cells to glide, even when they were previously induced. the ability of myxococcus xanthus to glide in groups using the s motility system but not as single cells (a system) was prevented by chloramphenicol and erythromycin.	1989	2509428
evidence for a membrane-associated gtp-binding protein in stigmatella aurantiaca, a prokaryotic cell.	signal transducing g proteins are present in all eukaryotic cells, but they have not been found in prokaryotes so far. myxobacteria, especially stigmatella aurantiaca, are prokaryotic organisms able to exchange signals. moreover, they exhibit an active phosphoinositide metabolism, whose intensity is dependent on the physiological state of the cell. therefore g proteins potentially involved in the activation of phospholipid metabolism or any other event stimulated by external signals were looked ...	1989	2492803
mutations that affect production of branched rna-linked msdna in myxococcus xanthus.	a deletion mutation of the gene (msd-msr) for the branched rna-linked msdna of myxococcus xanthus was constructed by replacing the chromosomal 0.7-kilobase (kb) smai-xhoi fragment encompassing msd-msr with a 1.4-kb fragment carrying a gene for kanamycin resistance. it was found that this deletion strain (delta mssx) could not produce msdna, although it still contained another species of msdna, mrdna (msdna, reduced size). no apparent differences between delta mssx and the wild-type strain were o ...	1988	2461359
biosynthesis and structure of stable branched rna covalently linked to the 5' end of multicopy single-stranded dna of stigmatella aurantiaca.	stigmatella aurantiaca, a gram-negative bacterium, contains approximately 500 copies per cell of a short single-stranded linear dna (multicopy single-stranded dna: msdna). this dna is attached to a branched rna (msdrna) by its 5' end. the entire sequence of msdrna was determined and found to consist of 76 bases. the msdna is linked at the 19th g residue of msdrna by a 2', 5' phosphodiester linkage. the coding region for msdrna (msr) is located downstream of the coding region for msdna (msd). the ...	1987	2431795
branched rna covalently linked to the 5' end of a single-stranded dna in stigmatella aurantiaca: structure of msdna.	stigmatella aurantiaca is a gliding, gram-negative bacterium that shows a spectacular fruiting body formation upon starvation of nutrient. this bacterium was found to contain approximately 500 copies per cell of a short single-stranded linear dna (multicopy single-stranded dna: msdna). the primary structure of msdna was determined and found to consist of 162 or 163 deoxyribonucleotides. its unique chromosomal gene was cloned and sequenced. the msdna was found to be attached to a branched rna by ...	1987	2431793
purification of the dna-dependent rna polymerase from the myxobacterium stigmatella aurantiaca.	the dna-dependent rna polymerase (ec 2.7.7.6) of the myxobacterium stigmatella aurantiaca has been purified. it shows three main polypeptide bands with apparent molecular weights of 146,000, 105,000, and 40,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. beta and beta' subunits of the s. aurantiaca polymerase were shown to migrate in the 146,000-molecular-weight polypeptide band and the main sigma factor was shown to migrate in the 105,000-molecular-weight band by using heterol ...	1992	1556092
cell surface modifications induced by calcium ion in the myxobacterium stigmatella aurantiaca.	calcium ion induces in the myxobacterium stigmatella aurantiaca the ability to glide on solid surfaces and to become cohesive (d. f. gilmore and d. white, j. bacteriol. 161:113-117, 1985; b. j. womack, d. f. gilmore, and d. white, j. bacteriol. 171:6093-6096, 1989). the addition of calcium ion to the growth medium resulted in the formation of extracellular fibrils, the appearance in the membrane fractions of a 30-kda protein, and the accumulation in a low-speed centrifugal pellet of 10 polypepti ...	1992	1522058
cloning and dna sequence of sigb gene of stigmatella aurantiaca.		1992	1475207
size and stability of the genomes of the myxobacteria stigmatella aurantiaca and stigmatella erecta.	genomic dna of stigmatella aurantiaca dw 4/3.1 was restricted with the rare-cutting endonucleases asei and spei. the restriction pattern derived is composed of 33 asei and 25 spei fragments, whose total size amounts to approximately 9,350 kbp. genomic fingerprint analysis of chromosomal dna from several s. aurantiaca isolates further revealed five completely different spei and asei fingerprints and one distinct fingerprint for stigmatella erecta. in addition, minor variations between the genome ...	1992	1400181
in vivo production of a stable single-stranded cdna in saccharomyces cerevisiae by means of a bacterial retron.	gram-negative bacteria such as myxococcus xanthus, stigmatella aurantiaca, and escherichia coli contain retroelements called retrons. retrons consist of the msr-msd region and the gene for reverse transcriptase (rt), which are essential for the production of the branched rna-linked ms-dna (multicopy single-stranded dna). in this study, we attempted to produce msdna in the yeast saccharomyces cerevisiae. retron ec67 from e. coli, which is responsible for the production of msdna-ec67, was cloned u ...	1992	1378616
similarity between the myxococcus xanthus and stigmatella aurantiaca reverse transcriptase genes associated with multicopy, single-stranded dna.	to determine the evolutional relationship of bacterial retroelements of myxococcus xanthus and stigmatella aurantiaca, the nucleotide sequence of 3,060 bases encompassing msr, msd, and the upstream region of msd (downstream of msr) of s. aurantiaca dw4 was determined and compared with the same region from m. xanthus. an open reading frame was found 92 bases upstream of msd which encoded a polypeptide of 480 amino acid residues having 73% identity with the reverse transcriptase of m. xanthus. tog ...	1992	1372604
carotenoid pigments of facultatively anaerobic spirochetes.	carotenoid pigments were purified from a previously undescribed, red, halophilic spirochete (spirochete rs1), and from spirochaeta aurantia strain j1. both spirochetes are facultative anaerobes and produce pigments when growing aerobically. the major pigments of the two spirochetes were identified by means of chromatographic analysis, absorption spectroscopy, hydride reduction, acetylation and silylation experiments, and mass spectrometry. it was concluded that the major pigment from spirochete ...	1975	1158846
scanning electron microscopy of fruiting body formation by myxobacteria.	scanning electron microscopy was used to follow fruiting body formation by pure cultures of chondromyces crocatus m38 and stigmatella aurantica. vegetative cells were grown on sp agar and then transferred to bonner salts agar for fructification. fruiting in both species commences with the formation of aggregation centers which resemble a fried egg in appearance. in chondromyces the elevated center or "yolk" region of the aggregation enlarges into a bulbous structure under which the stalk forms a ...	1975	811649
distribution of adenosine 5'-triphosphate (atp)-dependent hexose kinases in microorganisms.	a systematic study of adenosine triphosphate (atp)-dependent hexose kinases among microorganisms has been undertaken. sixteen hexose kinases of five major types were partially purified from 12 microorganisms and characterized with respect to specificity for sugar and nucleotide substrates and michaelis constants for the sugar substrates. glucokinase activities that phosphorylate glucose and glucosamine are inhibited by n-acetyl-glucosamine and xylose, were found to be present in the non-sulphur ...	1978	214181
[occurrence and distribution of fruiting body-forming myxobacteria in siebengebirge. comparative studies with special reference to characteristic biotypes].	in the region of the "siebengebirge" near bonn, six characteristic biotopes were studied with respect to the occurrence of fruiting body forming myxobacteria. 23 different species, belonging to the 8 known genera, were found. the number of species in the respective biotopes varied between 6 and 17, the average numbers of species per sampling area were between 2.1 and 10.0. 15 species were found on dung pellets of wild living herbivorous mammals. deciduous forest soils contained 13 species in spr ...	1979	121178
iso-branched 2- and 3-hydroxy fatty acids as characteristic lipid constituents of some gliding bacteria.	the fatty acids present in the total hydrolysates of several gliding bacteria (myxococcus fulvus, stigmatella aurantiaca, cytophaga johnsonae, cytophaga sp. strain samoa and flexibacter elegans) were analyzed by combined gas-liquid chromatography and mass spectrometry. in addition to 13-methyl-tetradecanoic acid, 15-methyl-hexadecanoic acid, hexadecanoic acid, and hexadecenoic acid, 2- and 3-hydroxy fatty acids comprised up to 50% of the total fatty acids. the majority was odd-numbered and iso-b ...	1979	118159
morphogenetic movements and multicellular development in the fruiting myxobacterium, stigmatella aurantiaca.		1978	109333
light-stimulated morphogenesis in the fruiting myxobacterium stigmatella aurantiaca.	when the fruiting myxobacterium stigmatella aurantiaca, a gliding prokaryote, is starved on an agar surface, the cells form multicellular aggregates resulting from morphogenetic movements. in the presence of incandescent light, each aggregate develops into a structurally complex fruiting body, possessing a stalk and several sporangia. in contrast, this pattern of development is not seen when cultures are incubated in the dark. the cells form irregular interconnecting aggregates, which rarely dev ...	1978	96528