Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a serological survey of antibodies against bovine herpesvirus-1 in yak (poephagus grunniens) in arunachal pradesh in india. | serum samples were collected from 254 yak (poephagus grunniens, presently bos grunniens) in different yak tracts of india. these samples were then screened by virus neutralisation test (vnt) and avidin-biotin enzyme-linked immunosorbent assay (ab-elisa) to study the seroprevalence of antibodies against bovine herpesvirus type 1 (bhv-1). the overall seroprevalence in yak was found to be 41% (105) by vnt and ab-elisa. the sex of the animal, whether it was on a farm or free-ranging and the location ... | 2009 | 20462162 |
| small noncoding rnas encoded within the bovine herpesvirus 1 latency-related gene can reduce steady-state levels of infected cell protein 0 (bicp0). | following acute infection in mucosal epithelium, bovine herpes virus 1 (bhv-1) establishes lifelong latency in sensory neurons within trigeminal ganglia. the latency-related rna (lr-rna) is abundantly expressed in sensory neurons of latently infected calves. expression of lr proteins is necessary for the latency reactivation cycle because a mutant virus that does not express lr proteins is unable to reactivate from latency after dexamethasone treatment. lr-rna sequences also inhibit bicp0 expres ... | 2010 | 20410286 |
| productive infection and bicp0 early promoter activity of bovine herpesvirus 1 are stimulated by e2f1. | bovine herpesvirus 1 (bohv-1) is an important viral pathogen of cattle. like other members of the subfamily alphaherpesvirinae, bohv-1 establishes latency in sensory neurons and has the potential to reactivate from latency. dexamethasone (dex) treatment of latently infected calves or rabbits consistently leads to reactivation from latency. the bohv-1 transcript encoding the infected cell protein 0 (bicp0) is consistently detected during reactivation from latency, in part because the bicp0 early ... | 2010 | 20410283 |
| erratum to: serological evidence of bovine herpesvirus-1 (bohv-1) infection in yaks (peophagus grunniens) from the national research centre on yak, india. | 2010 | 20390352 | |
| immunogenicity of a modified-live virus vaccine against bovine viral diarrhea virus types 1 and 2, infectious bovine rhinotracheitis virus, bovine parainfluenza-3 virus, and bovine respiratory syncytial virus when administered intranasally in young calves. | the immunogenicity of an intranasally-administered modified-live virus (mlv) vaccine in 3-8 day old calves was evaluated against bovine viral diarrhea virus (bvdv) types 1 and 2, infectious bovine rhinotracheitis (ibr) virus, parainfluenza-3 (pi-3) virus and bovine respiratory syncytial virus (brsv). calves were intranasally vaccinated with a single dose of a multivalent mlv vaccine and were challenged with one of the respective viruses three to four weeks post-vaccination in five separate studi ... | 2010 | 20381643 |
| bighorn sheep fetal lung cell line for detection of respiratory viruses. | pneumonia is an important disease of bighorn sheep (bhs) that is primarily responsible for the drastic decline in numbers of these animals in north america. members of the genus mannheimia and pasteurella have frequently been isolated from the pneumonic lungs of bhs. antibodies to several respiratory viruses, including bovine parainfluenza virus 3 (bpiv-3), bovine respiratory syncytial virus (brsv), bovine viral diarrhea virus (bvdv), and bovine herpesvirus 1 (bohv-1), have been detected in herd ... | 2010 | 20357964 |
| effect of foot-and-mouth disease virus capsid precursor protein and 3c protease expression on bovine herpesvirus 1 replication. | several reports have previously shown that expression of the foot-and-mouth disease virus (fmdv) capsid precursor protein encoding region p1-2a together with the 3c protease (p1-2a/3c) results in correct processing of the capsid precursor into vp0, vp1 and vp3 and formation of fmdv capsid structures that are able to induce a protective immune response against fmdv challenge after immunization using naked dna constructs or recombinant viruses. to elucidate whether bovine herpesvirus 1 (bhv-1) mig ... | 2010 | 20333533 |
| an indirect elisa for serodiagnosis of cattle footrot caused by fusobacterium necrophorum. | a serodiagnostic elisa (rl-elisa) using recombinant truncated leukotoxin protein pl2 (aa 311-644) of fusobacterium necrophorum as antigen was developed for detection of antibodies against f. necrophorum from cattle footrot. in rl-elisa, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type a, bovine escherichia coli, and bovine salmonella. the rl-elisa ... | 2010 | 20304080 |
| glycoprotein d of bovine herpesvirus 5 (bohv-5) confers an extended host range to bohv-1 but does not contribute to invasion of the brain. | bovine herpesvirus 1 (bohv-1) and bohv-5 are closely related pathogens of cattle, but only bohv-5 is considered a neuropathogen. we engineered intertypic gd exchange mutants with bohv-1 and bohv-5 backbones in order to address their in vitro and in vivo host ranges, with particular interest in invasion of the brain. the new viruses replicated in cell culture with similar dynamics and to titers comparable to those of their wild-type parents. however, gd of bohv-5 (gd5) was able to interact with a ... | 2010 | 20219909 |
| bovine herpesvirus 5 bicp0 complements the bovine herpesvirus 1 homolog. | bovine herpesvirus 1 (bohv-1) and bohv-5 are closely related (82% amino acid identity) but differ strongly in neuropathogenesis. the immediate-early gene for bicp0 is less conserved (70% amino acid identity) and may contribute to a dissimilar phenotype. a peculiar difference is a guanosine hexamer in the bicp0-1 gene which aligns with only five guanosines in the bicp0-5 gene and therefore results in a frameshift in the latter open reading frame. thus, the c-terminal amino acid sequence (residues ... | 2010 | 20211531 |
| should the domestic buffalo (bubalus bubalis) be considered in the epidemiology of bovine herpesvirus 1 infection? | only limited information is available on the epidemiology and pathogenesis of bovine herpesvirus 1 (bohv-1) in domestic buffalos. in this study, a virulent bohv-1 field strain isolated from cattle was inoculated into buffaloes to evaluate their susceptibility to the virus and to investigate the establishment of viral latency through clinical, virological and serological investigations. latency was also studied by attempting viral reactivation using pharmacological induction. six of seven male, 5 ... | 2010 | 20206452 |
| point mutations in bhv-1 us3 gene abolish its ability to induce cytoskeletal changes in various cell types. | the us3 gene is conserved among alphaherpesviruses and codes for a protein kinase, a multifunctional protein involved in many phases of virus infection, like nuclear egress, modulation of apoptosis and modification of the cellular cytoskeleton. bovine herpesvirus (bhv-1), a member of the alphaherpesvirinae, contains an open reading frame homologous to us3 of other herpesviruses, which has been identified as a serine/threonine kinase (takashima, y., tamura, h., xuan, x., otsuka, h., 1999. identif ... | 2010 | 20197221 |
| protein display by bovine herpesvirus type 1 glycoprotein b. | glycoprotein b (gb) of bovine herpesvirus 1 (bhv-1), a major component of the viral envelope, is essential for membrane fusion during entry and cell-to-cell spread. it is cleaved in the trans-golgi network by the proprotein convertase furin. integration of the open reading frame (orf) encoding a mutated gb with a second furin cleavage site and mature boifn-alpha as intervening peptide between the amino-terminal (nh(2)) and carboxy-terminal (cooh) gb subunits yielded recombinant bhv-1/gb2fuifn-al ... | 2010 | 20193990 |
| immunization of cattle with recombinant newcastle disease virus expressing bovine herpesvirus-1 (bhv-1) glycoprotein d induces mucosal and serum antibody responses and provides partial protection against bhv-1. | bovine herpesvirus-1 (bhv-1) is a major cause of respiratory tract diseases in cattle. vaccination of cattle against bhv-1 is a high priority. a major concern of currently modified live bhv-1 vaccines is their ability to cause latent infection and subsequent reactivation resulting in many outbreaks. thus, there is a need for alternative strategies. we generated two recombinant newcastle disease viruses (ndvs) expressing the glycoprotein d (gd) of bhv-1 from an added gene. one recombinant, rlasot ... | 2010 | 20189484 |
| serological evidence of bovine herpesvirus-1 (bohv-1) infection in yaks (peophagus grunniens) from the national research centre on yak, india. | bovine herpesvirus-1 (bohv-1) causes a variety of disease syndromes including, respiratory, nervous and reproductive disorders both in domestic as well as wild bovines and the disease is prevalent throughout the world including india. in this study, serum samples of yaks were screened for serological evidence of bohv-1 in a yak farm with history of abortion and kerato-conjuctivitis by competition-elisa. the result of seroprevalence of bohv-1 infections in yaks (poephagus grunniens) revealed that ... | 2010 | 20143159 |
| development and statistical validation of a guinea pig model for vaccine potency testing against infectious bovine rhinothracheitis (ibr) virus. | infectious bovine rhinothracheitis (ibr) caused by bovine herpesvirus 1 (bohv-1) infection is distributed worldwide. bohv-1 either alone or in association with other respiratory cattle pathogens causes significant economic losses to the livestock industry. the aim of this work was to validate a guinea pig model as an alternative method to the current bohv-1 vaccine potency testing in calves. guinea pigs were immunized with two doses of vaccine, 21 days apart and sampled at 30 days post vaccinati ... | 2010 | 20123054 |
| characterization of the nuclear import and export mechanisms of bovine herpesvirus-1 infected cell protein 27. | in previous study, we have identified a nuclear localization signal (nls) and a nucleolar localization signal (nols) in bovine herpesvirus-1 (bhv-1) infected cell protein 27 (bicp27), which targets predominantly to the nucleolus. furthermore, the c-terminal 300 amino acid residues targets exclusively to the cytoplasm, suggesting that bicp27 might contain a nuclear export signal (nes). amino acid sequence analysis revealed that there is a cluster of leucine-rich residues resembling a nes. heterok ... | 2010 | 20109505 |
| comparison of the biological and biochemical activities of several members of the alphaherpesvirus icp0 family of proteins. | immediate-early protein icp0 of herpes simplex virus type 1 (hsv-1) is an e3 ubiquitin ligase of the ring finger class that is required for efficient lytic infection and reactivation from latency. other alphaherpesviruses also express icp0-related ring finger proteins, but these have limited homology outside the core ring domain. existing evidence indicates that icp0 family members have similar properties, but there has been no systematic comparison of the biochemical activities and biological f ... | 2010 | 20106921 |
| critical role of cholesterol in bovine herpesvirus type 1 infection of mdbk cells. | cholesterol is involved in the life cycle of many viruses. here, we examined the role of cholesterol for both viral envelope and target cell membrane for bovine herpesvirus type 1 (bohv-1) infection. cholesterol depletion by pretreatment of madin-darby bovine kidney (mdbk) cells with a cholesterol-sequestering drug methyl-beta-cyclodextrin (mbetacd), inhibited the production of bohv-1 in a dose-dependent manner. this inhibitory effect was partially reversed by cholesterol replenishment, indicati ... | 2010 | 20097021 |
| surveys for disease agents in introduced elk in arkansas and kentucky. | surveys for disease agents were conducted in introduced free-ranging elk (cervus elaphus nelsoni) in arkansas and kentucky. elk had been captured in colorado and nebraska and released in arkansas during 1981-1985. from 1997 through 2002 elk were captured in arizona, kansas, north dakota, new mexico, oregon, and utah and released in southeastern kentucky. specimens were collected from 170 hunter-killed elk in arkansas during 1998-2006, and 44 elk in kentucky during 2001-2004. significant findings ... | 2010 | 20090032 |
| a bovine herpesvirus 5 recombinant defective in the thymidine kinase (tk) gene and a double mutant lacking tk and the glycoprotein e gene are fully attenuated for rabbits. | bovine herpesvirus 5 (bohv-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in south america and several efforts have been made to produce safer and more effective vaccines. in the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent brazilian bohv-5 strain (sv507/99). the recombinants are defective in glycoprotein e (bohv-5gedelta), thymidine kinase (bohv-5tkdelta) and both proteins (bohv ... | 2010 | 20027480 |
| bovine herpesvirus-1 us3 protein kinase: critical residues and involvement in the phosphorylation of vp22. | the us3 gene product of bovine herpesvirus-1 (bohv-1) is a protein kinase that is expressed early during infection and capable of autophosphorylation. by examining differentially labelled us3 moieties by co-immunoprecipitation, we demonstrated that the protein kinase interacts with itself in vitro, which supports autophosphorylation by us3. based on its homology to other serine/threonine protein kinases, we defined two highly conserved lysines in us3, at position 195 within the atp-binding pocke ... | 2010 | 20016039 |
| construction of single-chain fv with two possible cdr3h conformations but similar inter-molecular forces that neutralize bovine herpesvirus 1. | bovine herpesvirus 1 (bohv-1) causes respiratory and genital diseases in cattle for which available vaccines do not confer adequate protection. since passive immunization with antibodies permits disease prevention, single-chain fragment variable (scfv), originating from a monoclonal bovine igg1 antibody against bohv-1, were constructed and expressed in pichia pastoris in v(lambda)-v(h) orientation via a flexible seven-amino acid linker. similar to the intact igg, the purified recombinant scfv ne ... | 2010 | 20006385 |
| update on viral pathogenesis in brd. | many viruses, including bovine herpesvirus-1 (bhv-1), bovine respiratory syncytial virus (brsv), parainfluenzavirus-3 (pi3), bovine coronavirus, bovine viral diarrhea virus and bovine reovirus, have been etiologically associated with respiratory disease in cattle. this review focuses on the pathogenesis of bhv-1 and brsv, two very different agents that primarily cause disease in the upper and lower respiratory tract, respectively. | 2009 | 20003652 |
| host response to bovine respiratory pathogens. | bovine respiratory disease (brd) involves complex interactions amongst viral and bacterial pathogens that can lead to intense pulmonary inflammation (fibrinous pleuropneumonia). viral infection greatly increases the susceptibility of cattle to secondary infection of the lung with bacterial pathogens like mannheimia haemolytica and histophilus somni. the underlying reason for this viral/bacterial synergism, and the manner in which cattle respond to the virulence strategies of the bacterial pathog ... | 2009 | 20003650 |
| bovine respiratory disease research (1983-2009). | bovine respiratory disease (brd) research has provided significant understanding of the disease over the past 26 years. modern research tools that have been used include monoclonal antibodies, genomics, polymerase chain reaction, immunohistochemistry (ihc), dna vaccines and viral vectors coding for immunogens. emerging/reemerging viruses and new antigenic strains of viruses and bacteria have been identified. methods of detection and the role for cattle persistently infected bovine viral diarrhea ... | 2009 | 20003649 |
| inhibition of bovine herpesvirus multiplication in mdbk cells by small interfering rnas. | small interfering rna (sirna) mediated gene silencing is a promising approach in antiviral therapy. to investigate the antiviral effects of sirnas on bovine herpesvirus 1 (bohv-1) multiplication, we designed and in vitro synthesized two sirnas (sirna-1 and sirna-2) targeting the ul25 gene that is essential for bhv-1 multiplication. sirna-1 and sirna-2 inhibited the bohv-1 multiplication in mdbk cells to a different extent, namely by 11% and 40%, respectively, as demonstrated by virus titers (tci ... | 2009 | 19941403 |
| neutralizing antibodies to bovine herpesvirus types 1 (bohv-1) and 5 (bohv-5) and its subtypes. | this study was carried out to determine whether the sensitivity of serum neutralization (sn) tests would be affected by the use of distinct subtypes of bovine herpesvirus 1 (bohv-1) and 5 (bohv-5) as test challenge viruses. bovine sera collected from a randomized sample (n=287) were tested in a 24h incubation sn against three type 1 viruses (bohv-1.1 strains "los angeles" (la) and "evi 123"; bohv-1.2a strain "sv 265") and three type 5 viruses (bohv-5a strain "evi 88"; bohv-5b strain "a 663" and ... | 2010 | 19926411 |
| intracellular trafficking of vp22 in bovine herpesvirus-1 infected cells. | the intracellular trafficking of different vp22-enhanced yellow fluorescent protein (eyfp) fusion proteins expressed by bovine herpesvirus-1 (bhv-1) recombinants was examined by live-cell imaging. our results demonstrate that (i) the fusion of eyfp to the c terminus of vp22 does not alter the trafficking of the protein in infected cells, (ii) vp22 expressed during bhv-1 infection translocates to the nucleus through three different pathways, namely early mitosis-dependent nuclear translocation, l ... | 2010 | 19922972 |
| evaluation of safety and efficacy of dna vaccines against bovine herpesvirus-1 (bohv-1) in calves. | four dna vaccines against bohv-1 were evaluated for their efficacy in calves. twelve animals were divided into four groups which were injected with four different dna vaccines: pvax-tgd (vaccine a); pvax-tgd co-immunised with pvax-48cpg (vaccine b); pvax-ubilaci-tgd-l (vaccine c); pvax-ubilaci-tgd-l co-immunised with pvax-48cpg (vaccine d). three additional calves were given the plasmid vector and served as controls. ninety days after the first vaccination all calves were challenge infected with ... | 2011 | 19906427 |
| bovine herpesvirus type 1 as a novel oncolytic virus. | oncolytic virotherapy is a promising avenue of cancer gene therapy. current vectors include human viruses that have been engineered to replicate in tumor cells or nonhuman viruses that are naturally oncotropic and preferentially replicate in tumor cells harboring defects in innate immune pathways such as the type 1 interferon (ifn) pathway. bovine herpesvirus type 1 (bhv-1) is a species-specific herpesvirus closely related to the human herpes simplex virus type 1 (hsv-1). although bhv-1 does not ... | 2010 | 19893594 |
| a ul47 gene deletion mutant of bovine herpesvirus type 1 exhibits impaired growth in cell culture and lack of virulence in cattle. | tegument protein vp8 encoded by the u(l)47 gene of bovine herpesvirus type 1 (bhv-1) is the most abundant constituent of mature virions. in the present report, we describe the characterization of u(l)47 gene-deleted bhv-1 in cultured cells and its natural host. the u(l)47 deletion mutant exhibited reduced plaque size and more than 100-fold decrease in intracellular and extracellular viral titers in cultured cells. ultrastructural observations of infected cells showed normal maturation of bhv-1 v ... | 2010 | 19864376 |
| cloning of bovine herpesvirus type 1 and type 5 as infectious bacterial artifical chromosomes. | bovine herpesviruses type 1 (bohv1) and type 5 (bohv5) are two closely related pathogens of cattle. the identity of the two viruses on the amino acid level averages 82%. despite their high antigenetic similarities the two pathogens induce distinctive clinical signs. bohv1 causes respiratory and genital tract infections while bohv5 leads to severe encephalitis in calves. | 2009 | 19828032 |
| characterization of micrornas encoded by the bovine herpesvirus 1 genome. | bovine herpesvirus 1 (bohv-1) is a ubiquitous and important pathogen of cattle worldwide. this study reports the identification of 10 microrna (mirna) genes, bhv1-mir-b1-bhv1-mir-b10, encoded by the bohv-1 genome that were processed into 12 detectable mature mirnas as determined by ultra-high throughput sequencing bioinformatics analyses of small rna libraries and expression studies. we found that four of the mirna genes were present as two copies in the bohv-1 genome, resulting in a total of 14 ... | 2010 | 19793906 |
| comparison of bovine herpesvirus 1 vaccines for rapid induction of immunity. | the purpose of the study reported here was to compare four different vaccine regimens for their efficacy in protecting calves from challenge with bovine herpesvirus 1 (bhv1) at 5 and 14 days after vaccination. nine experimental groups of five calves each were used to compare four vaccination regimens and a group that received no vaccine. the four vaccine regimens were: a bhv1 intramuscular (im) vaccine containing both modified live virus (mlv) and killed virus (kv); an mlv intranasal (in) vaccin ... | 2000 | 19757568 |
| response to modified live and killed multivalent viral vaccine in regularly vaccinated, fresh dairy cows. | vaccination programs for viral pathogens in the dairy industry span the full spectrum of possibilities even though few of these have been evaluated in field situations. one such program is the vaccination of fresh cows 30 to 60 days postpartum with modified live viral (mlv) vaccines. the purpose of this study was to evaluate the antibody response to booster vaccinations during this period. the impact of vaccinations on milk production and reproductive performance was also examined. the response ... | 2000 | 19757565 |
| neutralizing antibody responses in preconditioned calves following vaccination for respiratory viruses. | calf preconditioning programs that include vaccinations against common respiratory pathogens are beneficial; however, optimal vaccination protocols have not been determined. in the study described here, calves were placed in a preconditioning program and assigned to one of nine vaccine regimens, each consisting of some combinations of killed or modified-live virus vaccines against bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, and bovine respirato ... | 2002 | 19750742 |
| comparison of single vaccination versus revaccination with a modified-live virus vaccine containing bovine herpesvirus-1, bovine viral diarrhea virus (types 1a and 2a), parainfluenza type 3 virus, and bovine respiratory syncytial virus in the prevention of bovine respiratory disease in cattle. | objective-to compare effects of administration of a modified-live respiratory virus vaccine once with administration of the same vaccine twice on the health and performance of cattle. design-randomized, controlled trial. animals-612 mixed-breed male cattle with unknown health histories. procedures-cattle were randomly assigned to 1 of 2 treatment groups (single vaccination treatment group [svac group] vs revaccination treatment group [revac group]) during the preconditioning phase of production. ... | 2009 | 19719452 |
| efficacy of a combination viral vaccine for protection of cattle against experimental infection with field isolates of bovine herpesvirus-1. | to determine whether a combination viral vaccine containing a modified-live bovine herpesvirus-1 (bhv-1) would protect calves from infection with virulent field strains of bhv-1 for weeks or months after vaccination. | 2009 | 19719449 |
| bovine herpesvirus: what is missing from our understanding of the relationship between bohv-1 and bohv-5? | 2010 | 19703787 | |
| major tegument protein vp8 of bovine herpesvirus 1 is phosphorylated by viral us3 and cellular ck2 protein kinases. | the ul47 gene product, vp8, is one of the major tegument proteins of bovine herpesvirus 1 (bohv-1) and is subject to phosphorylation. analysis of protein bands co-immunoprecipitated with vp8 from bohv-1-infected cells by mass spectroscopy suggested that vp8 interacts with two protein kinases: cellular ck2 and viral us3. ck2 is a highly conserved cellular protein, expressed ubiquitously and known to phosphorylate numerous proteins. the us3 gene product is one of the viral kinases produced by bohv ... | 2009 | 19692545 |
| seroconversion to bovine viral diarrhoea virus and infectious bovine rhinotracheitis virus in dairy herds of michoacan, mexico. | bovine viral diarrhoea virus (bvdv) and infectious bovine rhinotracheitis virus (ibrv) are important viral diseases around the world. the objective of this study was to estimate the incidence of seroconversion to bvdv and ibrv and to identify associated risk factors in dairy herds of michoacan, mexico. the longitudinal study included 62 herds and ran from december 2001 to november 2002. the total number of animals enrolled and completing the study were 392 and 342 animals for bvdv and 925 and 89 ... | 2010 | 19685278 |
| exposure to pathogens and incidence of respiratory disease in young bulls on their arrival at fattening operations in france. | the incidence of clinical respiratory disease in 698 young beef bulls kept in 68 pens, and their exposure to respiratory pathogens after their arrival at 51 fattening operations in western france were assessed. antibodies against bovine respiratory syncytial virus (brsv), bovine herpesvirus type 1 (bhv-1), mannheimia haemolytica and mycoplasma bovis were measured by elisa. the incidence risk of respiratory disease was 18.5 per cent during the first six weeks. cases occurred in 37 of the 68 pens, ... | 2009 | 19684344 |
| characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27. | bovine herpesvirus-1 infected cell protein 27 (bicp27) was detected predominantly in the nucleolus. the open reading frame of bicp27 was fused with the enhanced yellow fluorescent protein (eyfp) gene to investigate its subcellular localization in live cells and bicp27 was able to direct monomeric, dimeric or trimeric eyfp exclusively to the nucleolus. by constructing a series of deletion mutants, the putative nuclear localization signal (nls) and nucleolar localization signal (nols) were mapped ... | 2009 | 19682510 |
| prevalence of brucellosis and infectious bovine rhinotracheitis in organized dairy farms in india. | this study was carried out to investigate the prevalence of bovine brucellosis and infectious bovine rhinotracheitis (ibr) in organized dairy farms with history of abortion in india. elisa and rose bengal plate test (rbpt) were used to detect the seropositive animals and the test results indicated that 22.18% and 13.78% animals were declared as sero-positive by elisa and rbpt, respectively. milk ring test (mrt) was carried out only in one farm and 12.82% of the tested animals were turned positiv ... | 2010 | 19644761 |
| [construction and identification of recombinant bhv-1 expressing foot and mouth disease virus vp1 gene]. | in order to construct the recombinant bovine hepervirus-1 (bhv-1) which expressed foot and mouth disease virus (fmdv) vp1 gene, we constructed a bhv-1 ge gene transfer vector by inserting the synthetic vp1 gene of fmdv (o/china/99) under the immediate-early promoter of cytomegalovirus. | 2009 | 19637579 |
| repertoire of bovine mirna and mirna-like small regulatory rnas expressed upon viral infection. | microrna (mirna) and other types of small regulatory rnas play a crucial role in the regulation of gene expression in eukaryotes. several distinct classes of small regulatory rnas have been discovered in recent years. to extend the repertoire of small rnas characterized in mammals and to examine relationship between host mirna expression and viral infection we used illumina's ultrahigh throughput sequencing approach. we sequenced three small rna libraries prepared from cell line derived from the ... | 2009 | 19633723 |
| infertility and venereal disease in cattle inseminated with semen containing bovine herpesvirus type 5. | a group of 20 cows and heifers experienced poor conception rates and probable ovarian dysfunction after being artificially inseminated. when first examined, some showed signs of vulvovaginitis, with pustular, ulcerative lesions consistent with a herpesvirus infection. they had had no contact with bulls during the current breeding season. bovine herpesvirus type 5 (bhv-5) was isolated from samples of frozen semen from the batch that had been used for the artificial insemination programme. bhv-5 w ... | 2009 | 19633324 |
| effect of cryopreservation by slow cooling and vitrification on viral contamination of ivf embryos experimentally exposed to bovine viral diarrhea virus and bovine herpesvirus-1. | the objective was to determine the effect of cryopreservation by conventional slow controlled cooling (0.5 degrees c/min) and by vitrification on the presence of bovine viral diarrhea virus (bvdv) and bovine herpesvirus-1 (bhv-1) infectivity associated with frozen-thawed day 7 bovine embryos. in this study, day 7 embryos generated by in vitro fertilization (ivf) were exposed in vitro for 1.5h to bvdv (n=393) and bhv-1 (n=242) and subsequently tested before and after cryopreservation for the pres ... | 2009 | 19616294 |
| alphaherpesvirus infections in semidomesticated reindeer: a cross-sectional serological study. | alphaherpesviruses infect a wide range of animal species and cause diseases. cervid herpesvirus 2 (cvhv-2) was originally isolated from reindeer in finland but the impact of cvhv-2 infections on reindeer remains unclear. cvhv-2 infection could be partly responsible for calf losses as there are indications that it is associated with abortions and neonatal diseases. previous serosurveys of reindeer (rangifer tarandus tarandus) have shown that an alphaherpesvirus is circulating among reindeer in no ... | 2009 | 19604658 |
| serological response in cattle immunized with inactivated oil and algel adjuvant vaccines against infectious bovine rhinotracheitis. | infectious bovine rhinotracheitis (ibr) virus was grown in madin darby bovine kidney (mdbk) cell line using a roller culture system for its large-scale production. optimum multiplicity of infection (moi) of 1:750 was found to give consistent virus yield. to determine the appropriate payload, three batches of antigen with virus titres ranging from 10(8.37) to 10(6.37) tcid50 per ml were used to prepare experimental inactivated ibr oil adjuvant vaccine. beta-propiolactone (bpl) was used as inactiv ... | 2009 | 19579689 |
| evaluation of a blocking enzyme-linked immunosorbent assay for serological diagnosis of bovine herpesvirus 1. | bovine herpesvirus 1 (bohv-1) is an infectious agent of concern in the international export of bovine products; it is endemic in the united states, but it has been eradicated in many countries of the european union (eu). for export of semen to the eu, accurate assessment of bohv-1 status of the bull is required and is usually accomplished by measuring the level of antibody to the virus. the gold standard is virus neutralization (vn) using overnight incubation with the virus, a test approved by t ... | 2009 | 19564503 |
| lung pathology and infectious agents in fatal feedlot pneumonias and relationship with mortality, disease onset, and treatments. | this study charted 237 fatal cases of bovine respiratory disease (brd) observed from may 2002 to may 2003 in a single oklahoma feed yard. postmortem lung samples were used for agent identification and histopathology. late in the study, 94 skin samples (ear notches) were tested for bovine viral diarrhea virus (bvdv) by immunohistochemistry (ihc). bovine respiratory disease morbidity was 14.7%, and the mortality rate of all causes was 1.3%, with more than half (53.8%) attributed to brd (0.7% total ... | 2009 | 19564494 |
| high prevalence of co-infections with bovine herpesvirus 1 and 5 found in cattle in southern brazil. | based on small scale studies or on little sensitive serological tests, bovines in the south of the state of rio grande do sul, brazil, are known to be infected with either bovine herpesvirus 1 (bohv-1) or 5 (bohv-5). however, whether the prevalence of each of these viruses is high or low is currently still unknown. in order to determine the extent of bohv (-1 and/or -5) infections in bovines in this region of brazil, 200 bovines were studied for the presence of bohv dna. to this end, first a qua ... | 2009 | 19560292 |
| bovine herpes virus infections in cattle. | bovine herpes virus 1 (bhv-1) is primarily associated with clinical syndromes such as rhinotracheitis, pustular vulvovaginitis and balanoposthitis, abortion, infertility, conjunctivitis and encephalitis in bovine species. the main sources of infection are the nasal exudates and the respiratory droplets, genital secretions, semen, fetal fluids and tissues. the bhv-1 virus can become latent following a primary infection with a field isolate or vaccination with an attenuated strain. the viral genom ... | 2009 | 19558751 |
| dexamethasone treatment of calves latently infected with bovine herpesvirus 1 leads to activation of the bicp0 early promoter, in part by the cellular transcription factor c/ebp-alpha. | sensory neurons within trigeminal ganglia (tg) are the primary site for bovine herpesvirus 1 (bhv-1) latency. during latency, viral gene expression is restricted to the latency-related (lr) gene and the open reading frame orf-e. we previously constructed an lr mutant virus that expresses lr rna but not any of the known lr proteins. in contrast to calves latently infected with wild-type (wt) bhv-1 or the lr rescued virus, the lr mutant virus does not reactivate from latency following dexamethason ... | 2009 | 19553330 |
| characterization of interspecific recombinants generated from closely related bovine herpesviruses 1 and 5 through multiple pcr sequencing assays. | bovine herpesviruses 1 (bohv-1) and 5 (bohv-5) are closely related alphaherpesviruses infecting cattle. in countries where both viruses circulate, co-infection of cattle is likely. it was shown that recombination occurs at a high frequency in cattle infected dually with two bohv-1 mutants. in addition, interspecific recombinants are generated in cell culture co-infected with bohv-1 and bohv-5. even if the process of interspecific recombination appears inefficient relative to intraspecific recomb ... | 2009 | 19501619 |
| natural exposure of dromedary camels in sudan to infectious bovine rhinotracheitis virus (bovine herpes virus-1). | the occurrence of bovine herpes virus-1 (bhv-1) in camels was studied. a total of 186 pneumonic camel lungs were collected from slaughter houses at four different areas in sudan during 2000-2006. using sandwich elisa 1.6% of 186 tested lungs were found positive for bhv-1 antigen, all were from tambool at central sudan. direct fluorescent antibody test (fat) was used to confirm the bhv-1 elisa positives, all elisa positives were also positive. pcr was used to detect bhv-1 genome with three positi ... | 2009 | 19433051 |
| critical assessment of an in vitro bovine respiratory organ culture system: a model of bovine herpesvirus-1 infection. | a bovine in vitro organ culture (bivoc) system was evaluated as a model to study host and pathogen events during the course of bovine herpesvirus-1 infection. upper respiratory tract epithelium, from slaughtered animals, was cultured in an air-liquid interface system and integrity, viability, and tnf-alpha gene expression of tissue explants were monitored over 72h in the presence or absence of infection by bovine herpesvirus type 1 (bhv-1). uninfected explants maintained viability and integrity ... | 2009 | 19428580 |
| comparison of the pseudorabies virus us9 protein with homologs from other veterinary and human alphaherpesviruses. | pseudorabies virus (prv) us9 is a small, tail-anchored (ta) membrane protein that is essential for axonal sorting of viral structural proteins and is highly conserved among other members of the alphaherpesvirus subfamily. we cloned the us9 homologs from two human pathogens, varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1), as well as two veterinary pathogens, equine herpesvirus type 1 (ehv-1) and bovine herpesvirus type 1 (bhv-1), and fused them to enhanced green fluorescent ... | 2009 | 19420087 |
| biology of bovine herpesvirus 5. | bovine herpesvirus 5 (bohv-5) is an alphaherpesvirus responsible for meningoencephalitis in young cattle and is closely antigenically and genetically related to bovine herpesvirus 1 (bohv-1). both viruses have common aspects in their pathogenesis: (1) they infect epithelial cells at the portal of entry and (2) they establish a latent infection in the sensory nerve ganglia, i.e., the trigeminal ganglia. however, they have different neuroinvasion and neurovirulence capacities. only in rare cases c ... | 2010 | 19409823 |
| bovine herpesvirus type 1 infection of bovine bronchial epithelial cells increases neutrophil adhesion and activation. | respiratory infection of cattle with bovine herpesvirus type 1 (bhv-1) predisposes cattle to secondary pneumonia with mannheimia haemolytica as part of the bovine respiratory disease complex (brd). one cell type that has received limited investigation for its role in the inflammation that accompanies brd is the respiratory epithelial cell. in the present study we investigated mechanisms by which bhv-1 infection of respiratory epithelial cells contributes to the recruitment and activation of bovi ... | 2009 | 19406483 |
| modified bovine herpesvirus 1 for protein secretion. | the traditional way to utilize bovine herpesvirus 1 (bhv-1) and many other herpesviruses as vectors for synthesis of heterologous proteins like reporter proteins, antigens, or immunomodulatory active molecules was (and still is) the expression of the protein of interest from an entire gene consisting of promoter, 5'- and 3'-noncoding regions, the open reading frame (orf), and a signal sequence for polyadenylation. this approach is doubtlessly appropriate especially in cases when expression of la ... | 2009 | 19378128 |
| construction of a recombinant bhv-1 expressing the vp1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model. | foot-and-mouth disease (fmd) and infectious bovine rhinotracheitis (ibr) are two important infectious diseases of cattle. using bovine herpesvirus type 1 (bhv-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. in this study, a recombinant bhv-1 was constructed by inserting the synthetic fmdv (o/china/99) vp1 gene in the the ge locus of bhv-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phie cmv) and bovine ... | 2009 | 19343503 |
| granular vulvovaginitis syndrome in nelore pubertal and post pubertal replacement heifers under tropical conditions: role of mycoplasma spp., ureaplasma diversum and bhv-1. | in order to determine the role of mycoplasma spp, ureaplasma diversum and bhv-1 as causal agents of granular vulvovaginitis syndrome in nelore heifers raised under tropical conditions and based on the hypothesis that stressful conditions during puberty or breeding season would be a determinant factor for the infection, 340 heifers not vaccinated against bhv-1 were divided in post-pubertal, in the beginning of the first breeding season, and pubertal heifers. the vaginal lesion score (vls) grade 1 ... | 2009 | 19319656 |
| cervid herpesvirus 2 causes respiratory and fetal infections in semidomesticated reindeer. | members of the viral subfamily alphaherpesvirinae establish latency from which they can be reactivated. bovine herpesvirus 1 causes infectious bovine rhinotracheitis and infectious pustular vulvovaginitis in cattle, as well as abortion and weak calves. serological evidence of alphaherpesvirus infection has been reported for wild and semidomesticated reindeer (rangifer tarandus tarandus) in norway. to address the possibility that reindeer alphaherpesvirus (cervid herpesvirus 2 [cvhv-2]) infection ... | 2009 | 19279181 |
| enhanced immune response and protection efficacy of a dna vaccine constructed by linkage of the mycobacterium tuberculosis ag85b-encoding gene with the bvp22-encoding gene. | plasmid dna vaccines have been widely explored for use in tuberculosis immunization but their immunogenicity needs improvement. in the present study, we incorporated the bovine herpesvirus 1 vp22 (bvp22)-encoding gene, which encodes a protein that demonstrates a capability for disseminating the expressed antigen to neighbouring cells, into a dna vector in which it was fused to the ag85b-encoding gene of mycobacterium tuberculosis (mtb), and investigated whether this linkage could enhance immune ... | 2009 | 19273642 |
| evaluation of three commercial bovine elisa kits for detection of antibodies against alphaherpesviruses in reindeer (rangifer tarandus tarandus). | the genus varicellovirus (family herpesviridae subfamily alphaherpesvirinae) includes a group of viruses genetically and antigenically related to bovine herpesvirus 1 (bohv-1) among which cervid herpesvirus 2 (cvhv-2) can be of importance in reindeer. these viruses are known to be responsible for different diseases in both wild and domestic animals. reindeer are a keystone in the indigenous saami culture and previous studies have reported the presence of antibodies against alphaherpesviruses in ... | 2009 | 19272136 |
| [examination of biocides for their effectiveness against animal viruses according to european union standards with emphasis on the selection of a suitable test virus]. | because of the changes to be expected in the methods for testing disinfectants deemed to be used in the veterinary field, candidate viral species were evaluated for their suitability as test virus. considered viral species included different non-enveloped viruses [bovine enterovirus type 1 (ecbo (enteric cytopathogenic bovine orphan) virus), mammalian reovirus type 1, feline calici virus (fcv), and bovine parvovirus (bpv)], as well as enveloped viruses, as equine arteritisvirus (eav), bovine her ... | 2009 | 19226936 |
| a four year longitudinal sero-epidemiological study of bovine herpesvirus type-1 (bhv-1) in adult cattle in 107 unvaccinated herds in south west england. | bovine herpesvirus type-1 (bhv-1) is an important pathogen of cattle that presents with a variety of clinical signs, including the upper respiratory tract infection infectious bovine rhinotracheitis (ibr). a seroepidemiological study of bhv-1 antibodies was conducted in england from 2002 - 2004: 29,782 blood samples were taken from 15,736 cattle from 114 herds which were visited on up to three occasions. antibody concentration was measured using a commercial elisa. farm management information wa ... | 2009 | 19183476 |
| the infected cell protein 0 encoded by bovine herpesvirus 1 (bicp0) associates with interferon regulatory factor 7 and consequently inhibits beta interferon promoter activity. | the bicp0 protein encoded by bovine herpesvirus 1 stimulates productive infection and viral gene expression but inhibits interferon (ifn)-dependent transcription. bicp0 inhibits beta ifn (ifn-beta) promoter activity and induces degradation of ifn regulatory factor 3 (irf3). although bicp0 inhibits the trans-activation activity of irf7, irf7 protein levels are not reduced. in this study, we demonstrate that bicp0 is associated with irf7. furthermore, bicp0 inhibits the ability of irf7 to trans-ac ... | 2009 | 19176627 |
| coinfection with two closely related alphaherpesviruses results in a highly diversified recombination mosaic displaying negative genetic interference. | phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. however, despite an ever-increasing amount of evidence of recombination in herpesvirus history, the recombination process and the consequences on the genetic diversity of the progeny remain poorly characterized. we addressed this issue by using multiple single-nucleotide polymorphisms (snps) differentiating the two subtypes of an alphaherpesvirus, bo ... | 2009 | 19153224 |
| amended recombinant cells (arcs) expressing bovine ifn-gamma: an economical and highly effective adjuvant system. | interferon-gamma (ifn-gamma) has potent immune stimulatory activity, but purifying recombinant protein is expensive, soluble cytokine delivery is inefficient, and high doses of ifn-gamma cause adverse systemic effects. we've shown, however, that chemically fixed, amended recombinant pseudomonas fluorescens cells (arcs), expressing soluble bovine ifn-gamma (bgi/arcs), provide an effective production and delivery vehicle for highly active cytokine. in this report we investigate the immune enhancin ... | 2009 | 19150640 |
| the cellular transcription factor, ccaat enhancer-binding protein alpha (c/ebp-alpha), has the potential to activate the bovine herpesvirus 1 immediate-early transcription unit 1 promoter. | following acute infection, bovine herpesvirus-1 (bhv-1) establishes a lifelong latent infection in sensory neurons of trigeminal ganglia. bhv-1 periodically reactivates from latency and is shed as infectious virus. the latency-related (lr) gene is abundantly expressed in trigeminal ganglia of infected calves, and proteins encoded by the lr gene are necessary for reactivation from latency. we previously demonstrated that a novel lr protein interacts with a host transcription factor, ccaat enhance ... | 2009 | 19115128 |
| bovine herpesvirus type 1 (bohv-1) anterograde neuronal transport from trigeminal ganglia to nose and eye requires glycoprotein e. | the requirement of bovine herpesvirus type 1 (bohv-1) envelope protein ge (us8 homolog) for establishment of latency and reactivation in trigeminal ganglia (tg) was examined. although bhv-1 ge-rescued and ge-deleted viruses were isolated from nasal or ocular swabs during primary infection, only the ge-rescued virus was isolated following dexamethasone-induced reactivation. furthermore, gc protein expression, which requires viral dna replication for its expression, was detected in tg of calves in ... | 2009 | 19115127 |
| antibody response against three widespread bovine viruses is not impaired in holstein cattle carrying bovine leukocyte antigen drb3.2 alleles associated with bovine leukemia virus resistance. | due to the wide dissemination of bovine leukemia virus (blv) infection among dairy cattle, control and eradication programs based on serological detection of infected cattle and subsequent culling face a major economic task. in argentina, genetic selection of cattle carrying alleles of the bovine leukocyte antigen (bola) drb3.2 gene associated with blv-infection resistance, like *0902, emerges as the best additional tool toward controlling virus spread. a potential risk in expanding or segregati ... | 2009 | 19109295 |
| multiple molecular regions confer intracellular localization of bovine toll-like receptor 8. | toll-like receptors (tlrs), a family of highly conserved germline-encoded pattern-recognition receptors (prrs), are essential for the host immune response. the cellular localization of tlr proteins determines the access to certain sources of ligands and thus the triggering of downstream cellular signaling. the tlr7/8/9 subfamily proteins are localized intracellularly but the molecular elements determining the cellular localization of these proteins are not fully understood. here we demonstrated ... | 2009 | 19019441 |
| attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions. | the present study describes a novel microcarrier substrate consisting of a swellable, copolymer of styrene and divinylbenzene, derivatized with trimethylamine. the co-polymer trimethylamine microcarriers support the growth of a number of different cell lines - madin darby bovine kidney, madin-darby canine kidney, vero and cos-7 - under serum-free conditions, and human diploid fibroblasts in serum-containing medium. cells attach to the co- polymer trimethylamine microcarriers as rapidly as they a ... | 1998 | 19003412 |
| serum-free produced bovine herpesvirus type 1 and bovine parainfluenza type 3 virus vaccines are efficacious and safe. | the studies described in this report were performed to determine, whether it is possible to produce live virus vaccines without serum or fractions thereof used during any cell or virus passage, thus completely serum-free. two viruses were included in the experiments: bovine herpesvirus 1 (bhv-1) and bovine parainfluenza type 3 virus (pi3). both viruses were found to grow to satisfactory titers, and to be stable after freeze-drying and subsequent storage at temperatures of +4 degrees c and -20 de ... | 2002 | 19003306 |
| evaluation of trypsin treatment on the inactivation of bovine herpesvirus type 1 on in vitro produced pre-implantation embryos. | the aim of this study was to evaluate the efficiency of trypsin treatment on the inactivation of bovine herpesvirus type 1 (bohv-1) on in vitro produced by fertilization and artificially infected bovine embryos. bovine embryos on day 7 were exposed with 10 microl of bohv-1, los angeles strain 10(7.5) tcid. these embryos and control embryos were divided in two groups: submitted to the sequential washes or to the trypsin treatment according to the international embryo transfer society (iets) guide ... | 2009 | 18992124 |
| prevalence of infectious agents in free-ranging white-tailed deer in northeastern mexico. | the objectives of this study were to determine the prevalence of antibodies against brucellosis, leptospirosis, infectious bovine rhinotracheitis virus, and bovine viral diarrhea virus (bvdv) in white-tailed deer (odocoileus virginianus) in northeastern mexico. deer (n=521) were captured from helicopter using a netgun on 15 ranches covering 62,114 ha in the states of coahuila, nuevo leon, and tamaulipas during spring 2004. the prevalence of antibodies against leptospira, infectious bovine rhinot ... | 2008 | 18957659 |
| the essential and non-essential genes of bovine herpesvirus 1. | bovine herpesvirus 1 (bohv-1) is an economically important pathogen of cattle associated with respiratory and reproductive disease. to further develop bohv-1 as a vaccine vector, a study was conducted to identify the essential and non-essential genes required for in vitro viability. random-insertion mutagenesis utilizing a tn5 transposition system and targeted gene deletion were employed to construct gene disruption and gene deletion libraries, respectively, of an infectious clone of bohv-1. tra ... | 2008 | 18931083 |
| comparison of the biobullet versus traditional pharmaceutical injection techniques on injection-site tissue damage and tenderness in beef subprimals. | the incidence and severity of injection-site lesions has decreased since the development of the beef quality assurance program. the objective was to evaluate the route of administration and the pharmaceutical product on the impact on tenderness, collagen concentration, and lesion occurrence in muscles of chucks and rounds. a total of 144 yearling steers (initial bw = 383 +/- 29.4 kg) were selected and transported to oklahoma state university. steers were blocked into 2 groups of 72 based on init ... | 2009 | 18849383 |
| the zinc ring finger of bovine herpesvirus 1-encoded bicp0 protein is crucial for viral replication and virulence. | bovine herpesvirus 1 (bhv-1) infected cell protein 0 (bicp0) stimulates productive infection, in part by activating viral gene expression. the c(3)hc(4) zinc ring finger of bicp0 is crucial for activating viral transcription and productive infection. in this study, we used a bacterial artificial chromosome containing a wild-type (wt) virulent bhv-1 strain to generate a single amino acid mutation in the c(3)hc(4) zinc ring finger of bicp0. this virus (the 51g mutant) contains a cysteine-to-glycin ... | 2008 | 18842710 |
| serosurveillance of viral diseases in korean native goats (capra hircus). | a total of 804 goat sera were collected from 144 goat farms in five regions of south korea during a period between 2005 and 2006 and screened for the antibodies of viral pathogens in ruminants. the individual seropositive rates for each virus were 13.7% (110/804) for bovine herpesvirus-1 (bhv-1), 9.5% (76/804) for bovine parainfluenza type-3 virus (pi-3v), 5.5% (44/804) for akabane virus (akav), 13.3% (107/804) for aino virus (ainv), 2.0% (16/804) for chuzan virus (chuv) and 1.0% (8/804) for bov ... | 2008 | 18840974 |
| double immunization strategy with a bohv-4-vectorialized secreted chimeric peptide bvdv-e2/bohv-1-gd. | a bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. so cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrhoea virus glycoprotein e2 ectodomain with the bovine herpesvirus 1 glycoprotein d ectodomain. recombinant virus infected cells robustly expressed and secreted the chimeric peptide i ... | 2008 | 18812200 |
| the varicellovirus ul49.5 protein blocks the transporter associated with antigen processing (tap) by inhibiting essential conformational transitions in the 6+6 transmembrane tap core complex. | tap translocates virus-derived peptides from the cytosol into the endoplasmic reticulum, where the peptides are loaded onto mhc class i molecules. this process is crucial for the detection of virus-infected cells by ctl that recognize the mhc class i-peptide complexes at the cell surface. the varicellovirus bovine herpesvirus 1 encodes a protein, ul49.5, that acts as a potent inhibitor of tap. ul49.5 acts in two ways, as follows: 1) by blocking conformational changes of tap required for the tran ... | 2008 | 18802093 |
| bovine herpesvirus type 1 (bhv-1) and bovine viral diarrhoea virus (bvdv) infections in dairy herds: self clearance and the detection of seroconversions against a new atypical pestivirus. | the epidemiology of bovine herpesvirus type 1 (bhv-1) and bovine viral diarrhoea virus (bvdv) was studied in a population of small dairy herds that had not been vaccinated. bulk tank milk samples of 186 herds in thailand were collected four times between 2002 and 2004. serum samples from individual animals in 11 herds were also taken on three occasions. the prevalence of bhv-1 in the 186 herds was 61% in 2002, decreasing to 48% in 2004 and for bvdv was 91% in 2002, decreasing to 72% in 2004. a b ... | 2009 | 18760643 |
| the ul49 gene product of bohv-1: a major factor in efficient cell-to-cell spread. | the role of the ul49 gene product, vp22, of bovine herpesvirus type 1 (bohv-1) in virus replication was characterized with respect to a putative functional interaction of vp22 with the viral glycoprotein e (ge) during bohv-1 cell-to-cell spread. deletion of the open reading frames of ul49 and/or ge from an infectious bohv-1 bacterial artificial chromosome clone did not severely impair the production of viral progeny in single-step growth experiments. however, plaque sizes induced by a vp22-negat ... | 2008 | 18753236 |
| open reading frame 2, encoded by the latency-related gene of bovine herpesvirus 1, has antiapoptotic activity in transiently transfected neuroblastoma cells. | the latency-related (lr) rna encoded by bovine herpesvirus 1 is abundantly expressed in the trigeminal ganglia of latently infected calves. expression of lr proteins is necessary for reactivation from latency and the protection of infected neurons from apoptosis. in this study, we demonstrated that an lr-encoded protein, open reading frame 2 (orf-2), or orf-2 fusion proteins encoded by alternatively spliced lr transcripts inhibit cold shock or fas ligand-induced apoptosis in mouse neuroblastoma ... | 2008 | 18715935 |
| 2,3,7,8-tetrachlorodibenzo-p-dioxin regulates bovine herpesvirus type 1 induced apoptosis by modulating bcl-2 family members. | exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd), leads to an increased susceptibility to infectious agents. infection of bovine cells (mdbk) with bovine herpesvirus 1 (bhv-1) anticipates virus-induced apoptosis, suggesting an involvement of tcdd in virus infection. herein we analyzed the effects of tcdd on apoptotic pathway in mdbk cells infected with bhv-1. after 12 h of infection, tcdd induced a significant increase in apoptotic cells. tcdd caused a dose ... | 2008 | 18696228 |
| effect of stress on viral-bacterial synergy in bovine respiratory disease: novel mechanisms to regulate inflammation. | the severity of bovine respiratory infections has been linked to a variety of factors, including environmental and nutritional changes, transportation, and social reorganization of weaned calves. fatal respiratory infections, however, usually occur when a primary viral infection compromises host defences and enhances the severity of a secondary bacterial infection. this viral-bacterial synergy can occur by a number of different mechanisms and disease challenge models have been developed to analy ... | 2005 | 18629190 |
| microarray-based detection of viruses causing vesicular or vesicular-like lesions in livestock animals. | definitive diagnosis of vesicular or vesicular-like lesions in livestock animals presents challenges both for veterinary clinicians and diagnostic laboratories. it is often impossible to diagnose the causative disease agent on a clinical basis alone and difficult to collect ample vesicular epithelium samples. due to restrictions of time and sample size, once laboratory tests have ruled out foot-and-mouth disease, vesicular stomatitis and swine vesicular disease a definitive diagnosis may remain ... | 2009 | 18621489 |
| effect of age at the time of vaccination on antibody titers and feedlot performance in beef calves. | to compare antibody responses, feedlot morbidity and mortality rates, feedlot performance, and carcass value for calves vaccinated with 1 of 2 vaccination strategies and for unvaccinated control calves. | 2008 | 18593324 |
| associations between health and productivity in cow-calf beef herds and persistent infection with bovine viral diarrhea virus, antibodies against bovine viral diarrhea virus, or antibodies against infectious bovine rhinotracheitis virus in calves. | to measure associations between health and productivity in cow-calf beef herds and persistent infection with bovine viral diarrhea virus (bvdv), antibodies against bvdv, or antibodies against infectious bovine rhinotracheitis (ibr) virus in calves. | 2008 | 18593246 |
| lactoferrin from bovine milk inhibits bovine herpesvirus 1 in cell culture but suppresses development of in vitro-produced bovine embryos. | bovine herpesvirus 1 (bohv-1) is widely distributed among cattle populations and has been associated with cells, fluids, and tissues collected from donor animals for use in reproductive technologies. the purpose of this study was to determine if lactoferrin would inhibit bohv-1 in cell culture and to evaluate if embryos could develop normally when cultured in vitro with lactoferrin. in experiment 1, lactoferrin (10 mg/ml) inhibited up to 25,000 plaque forming units (pfu)/ml of bohv-1 in madin da ... | 2009 | 18586420 |
| seroprevalence of bovine respiratory viruses in north-western turkey. | bovine respiratory disease complex is a very important health problem around the world. present study describes serological distribution of bovine major respiratory viruses in non-vaccinated cattle population of marmara region in north-western turkey. neutralising antibodies specific to bovine viral diarrhoea virus (bvdv), bovine herpesvirus 1 (bhv-1), bovine respiratory syncytial virus (brsv), bovine parainfluenza virus 3 (pi-3), bovine adenovirus serotype 1 (bav-1) and serotype 3 (bav-3) were ... | 2008 | 18551779 |
| varicellovirus ul 49.5 proteins differentially affect the function of the transporter associated with antigen processing, tap. | cytotoxic t-lymphocytes play an important role in the protection against viral infections, which they detect through the recognition of virus-derived peptides, presented in the context of mhc class i molecules at the surface of the infected cell. the transporter associated with antigen processing (tap) plays an essential role in mhc class i-restricted antigen presentation, as tap imports peptides into the er, where peptide loading of mhc class i molecules takes place. in this study, the ul 49.5 ... | 2008 | 18516302 |
| a comparison of 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever/bovine respiratory disease. | the aim of this study was to compare 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever (uf)/bovine respiratory disease (brd). at feedlot arrival, 3882 calves were enrolled in the study and randomly allocated to 2 groups, which were housed by group in 12 pens. at the time of allocation, 1 group (mlv3-bt2) received a multivalent, modified-live viral vaccine containing infectious bovine rhinotracheitis virus (ibrv) and types i and ii bovine viral diarr ... | 2008 | 18512457 |
| [immune evasion of alphaherpesviruses]. | alphaherpesviruses represent the largest subfamily of the herpesviruses and comprise many different, closely related pathogens of man and animal, including herpes simplex virus (cold sores, genital lesions) and varicella-zoster virus (chickenpox, shingles) in man, pseudorabies virus oraujeszky's disease virus in pigs (neurological and respiratory symptoms, abortion), equine herpesvirus type 1 (neurological and respiratory symptoms, abortion), and bovine herpesvirus type 1 (respiratory symptoms, ... | 2008 | 18512358 |