Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| ascending and intrinsic projections of the superior olivary complex in the cat. | the ascending and intrinsic projections of the superior olivary complex (so) in the cat were investigated by injection of 3h-leucine and horseradish peroxidase (hrp) in so and the inferior colliculus (ic), respectively. a topically arranged projection was demonstrated from the nucleus of the trapezoid body (ntb) to the ipsilateral lateral superior olivary nucleus (lso) wiht a lesser connection in the opposite direction. the medial superior olivary nucleus (mso) has a strictly ipsilateral project ... | 1978 | 658183 |
| [chemical modification of epsilon-nh2 groups of lysine residues in horseradish peroxidase. accessibility of these groups to different modifying agents]. | modification of epsilon-nh2 groups of lysine residues in horseradish peroxidase by acetic, propionic, butyric, valeric and succinic anhydrides and trinitrobenzenesulfonic acid (tnbs) is carried out. all the anhydrides modify at 0 degrees c four epsilon-nh2 lysine groups, and tnbs--three groups. tnbs modifies all 6 peroxidase epsilon-nh2 groups at 40 degrees c. possible spacing of lysine epsilon-nh2 groups in the enzyme molecule is discussed. | 1978 | 656503 |
| [demonstration of afferent connections of the forebrain in emys orbicularis turtles by the peroxidase method]. | the transport of horseradish peroxidase (hrp) out of the injection site in the dorsal ventricular ridge was studied in turtles emys orbicularis. labeled cells in the forebrain were observed in the paleostriatum among fibers of the lateral forebrain bundle. in the thalamus most of cells containing the granular hrp reaction product were located in the n. rotundus, n. reuniens and perirotundal nuclei (n. dorso-medialis anterior, n. magnocellularis thalami, n (centralis) lateralis, n. dorso-medialis ... | 2010 | 654713 |
| glomerular filtration of proteins: clearance of anionic, neutral, and cationic horseradish peroxidase in the rat. | glomerular permeability to horseradish peroxidase, a protein slightly smaller than rat albumin but similar in shape, was studied in wistar-furth rats by using a purified neutral isozyme (hrp; molecular radius, a(e) = 29.8 a) as well as an anionic succinyl-derivative (shrp a(e) = 31.8 a) and a cationized enzyme (chrp, a(e) = 30 a). the clearance rate of the proteins was determined over a 20-min period using the amounts of enzyme actually filtered (i.e., protein in the urine and protein reabsorbed ... | 1978 | 651127 |
| horseradish peroxidase uptake into the rat choroid plexus epithelium, with special reference to the lysosomal system. | 1978 | 650732 | |
| abnormal transport of horseradish peroxidase from the medial gastrocnemius muscle in dystrophic mice [proceedings]. | 1978 | 650592 | |
| studies of pulmonary innervation in the rat using horseradish peroxidase as a neuronal marker [proceedings]. | 1978 | 650517 | |
| morphology and organization of axon collaterals from afferent fibres of slowly adapting type i units in cat spinal cord. | 1. the morphology of the collaterals of single axons innervating type i slowly adapting receptors was studied by using the intra-axonal injection of the enzyme horseradish peroxidase in anaesthetized cats. the axons were impaled near their dorsal root entrance zone in the lumbosacral cord. the morphology was revealed by subsequent histochemistry. 2. thirteen type i axons were stained, nine with receptors in the hairy skin and four with receptors in the glabrous foot pad skin. twelve axons could ... | 1978 | 650514 |
| comparison of methods for demonstrating retrogradely transported horseradish peroxidase in neurones [proceedings]. | 1978 | 650512 | |
| cytoarchitecture and thalamic connectivity of third somatosensory area of cat cerebral cortex. | 1. the third somatosensory area (siii) was identified in the cat cerebral cortex by the recording of surface potentials evoked by deflection of a single contralateral mystacial vibrissa. a small amount of tritiated leucine was then injected at the center of the focus of evoked activity and, after a suitable survival period, the brain was prepared for autoradiography. 2. as defined by the presence of an autoradiographic injection, the siii focus lay in a cytoarchitectonic field characterized in p ... | 1978 | 650267 |
| neuronal ceroid-lipofuscinosis. studies of granulocyte enzyme activities. | neuronal ceroid-lipofuscinosis is characterized by pigmentary degeneration of the retina, psychomotor degeneration, epilepsy and intracellular deposition of ceroidlipofuscin. recent reports have suggested that deficiency of peroxidase is the basic genetic defect. however, deficiency of myeloperoxidase could be demonstrated in some but not all patients; this deficiency was noted only when p-phenylenediamine (ppd) was used as hydrogen donor and could not be confirmed with guaiacol. we found that h ... | 1978 | 650251 |
| the permeability of a keratinizing squamous epithelium in culture. | horseradish peroxidase or lanthanum was applied to the surface of keratinized oral epithelium growing in tissue culture and the extent of penetration of these substances examined with the electron microscope. both tracer substances penetrated between the superficial keratinized squames of the tissue, the lanthanum reaching the basal cell layer and the peroxidase diffusing to within 3--8 cells of the basal layer. the permeability of the keratinized layer in this epithelium is in contrast to the s ... | 1978 | 649491 |
| [degradation of the parathyroid hormone in the kidney and liver (author's transl)]. | the cleavage of the parathyroid hormone has been reported to take place in various peripheral tissues, especially in the kidney and liver. in order to claify the mechanism of such a degradation, bovine pth (bpth 1--84) and its synthetic n-terminal peptide (bpth 1--34) labelled with 125i by the chroramine-t method (125i-bpth 1--84) and (125i-bpth 1--34) or labelled with horseradish peroxidase pox-125i-bpth 1--84 and pox 125i-bpth 1 34) were used to study the disappearance from the blood stream an ... | 1978 | 648683 |
| identification of the trochlear motoneurons by retrograde transport of horseradish peroxidase. | 1978 | 648617 | |
| multiple branching of cerebellar efferent projections in cats. | the retrograde labeling of neurons in the deep cerebellar nuclei with horseradish peroxidase was used to compare the morphological characteristics of neurons in the dentate and interposed nuclei projecting in the cerebellothalamic, cerebello-olivary, and cerebellar nucleocortical pathways. the results from these studies demonstrated that cerebellothalamic and nucleocortical projections from the dentate and interposed nuclei originate from similar populations of spindle- and multipolar-shaped neu ... | 1978 | 648599 |
| prosencephalic pathways related to the paleostriatum of the pigeon (columba livia). | afferent connections of the avian paleostriatal complex were traced by means of anterograde and retrograde transport of horseradish peroxidase (hrp). the paleostriatum augmentatum (pa), a cell field comparable to mammalian caudate nucleus and putamen, was found to receive projections from a distinct population of elencephalic neurons in the temporal-parietal-occipital (tpo) and lateral cortical (cdl) areas of the neostriatum. tpo neurons, in turn, were found to receive projections from the contr ... | 1978 | 647399 |
| preparation of protein conjugates via intermolecular disulfide bond formation. | conjugates of two unlike proteins can be prepared via the intermolecular disulfide interchange reaction, namely, protein a containing thiol groups reacts with protein b containing 4-dithiopyridyl groups to yield a conjugate with the release of 4-thiopyridone. thiol groups can be introduced into proteins upon amidination with methyl 3-mercaptopropionimidate ester or 2-iminothiolane, and 4-dithiopyridyl groups can be introduced into proteins with these same reagents in the presence of 4,4'-dithiod ... | 1978 | 646998 |
| afferents to brain stem nuclei (brain stem raphe, nucleus reticularis pontis caudalis and nucleus gigantocellularis) in the rat as demonstrated by microiontophoretically applied horseradish peroxidase. | using a retrograde tracer technique with microiontophoretically applied horseradish peroxidase (hrp), afferent projections to the brain stem raphe nuclei (br, raphe magnus, pallidus and obscurus) and to two adjacent reticular nuclei, nucleus reticularis pontis caudalis (nrpc) and nucleus gigantocellularis (ngc) were identified. the most striking difference between the afferent projections to the br and the adjacent nuclei as determined by this method is that afferents to the br originate primari ... | 1978 | 646855 |
| alterations in rat jejunal permeability to a macromolecular tracer during a hyperosmotic load. | the effects of intraluminal hyperosmolality on the intestinal permeability of a macromolecular tracer, horseradish peroxidase, was assessed in rats by light and electron microscopic cytochemistry. after 60 minutes of an intraluminal hypertonic perfusion of 600 mosm mannitol, horseradish peroxidase was demonstrable in the intercellular spaces between adjacent absorptive epithelial cells of jejunal villi, along the microvillar brush border, in numerous pinocytotic vesicles, in multivesicular bodie ... | 1978 | 642452 |
| oogenesis in xenopus laevis (daudin). vi. the route of injected tracer transport in the follicle and developing oocyte. | in xenopus laevis, vitellogenin (the yolk precursor) is synthesized in the liver and transported via the circulatory system to the ovary. in order to reach developing oocytes where it is sequestered, it must exit the circulatory system and traverse several follicular tissue layers including the theca, the follicle cell layer, and the vitelline envelope. this study demonstrates this pathway by means of electron-opaque tracers, and follows the fate of heterologous macromolecules after their incorp ... | 1978 | 641487 |
| extraocular muscle representation in the brainstem of the carp. | the representation of the extraocular muscles in the oculomotor nuclei in the brainstem of the carp was studied with the horseradish peroxidase technique. some normal anatomical features of the various oculomotor nuclei are described. following intramuscular hrp injections in the eye muscles and applying survival times of up to 25 days labeled perikarya were found in the various nuclei of the oculomotor complex. rectus inferior, rectus internus, and obliquus inferior are represented in the ipsil ... | 1978 | 641230 |
| the morphology of the spinal cord efferent and afferent neurons contributing to the ventral roots of the cat. | horseradish peroxidase was applied to proximal ventral roots of the coccygeal and sacral spinal cord of cats. subsequent histochemical reaction resulted in extensive staining of spinal cord neurons that had processes in the ventral roots. this procedure was used to study four issues concerning ventral root neurons. (1) extensive transverse dendritic arborizations were revealed for large and small neurons presumed to be alpha and gamma motoneurons respectively. dendrites from these neurons were f ... | 1978 | 641227 |
| superior colliculus connections with the extraocular motor nuclei in the cat. | direct and indirect projections from the cat superior colliculus to the extraocular motor nuclei were studied using the orthograde autoradiographic tracing method, the retrograde horseradish peroxidase technique, and golgi methods. the results show that the superior colliculus projects to the central gray matter directly overlying the oculomotor complex. this projection arises almost entirely from the rostral third of the colliculus, and it terminates most heavily over the rostral half of the oc ... | 1978 | 641226 |
| the vestibular nuclei in the domestic hen (gallus domesticus) iii. ascending projections to the mesencephalic eye motor nuclei. | following injections of horseradish peroxidase in the oculomotor and the trochlear nuclei in the hen, the occurrence of labeled cells was plotted in the vestibular nuclei. the majority of labeled cells was localized in the superior, the medial, and the tangential nucleus. within the superior nucleus the cells were found mainly caudally, extending medially and ventrally in central areas. in the medial nucleus labeled cells were localized exclusively in its rostral half, mainly in ventrolateral re ... | 1978 | 641224 |
| the organization of olivo-cerebellar projections in the opossum, didelphis virginiana, as revealed by the retrograde transport of horseradish peroxidase. | the retrograde transport of horseradish peroxidase was utilized to map olivo-cerebellar projections in the virginia opossum. the spinal cerebellum (anterior lobe, paramedian lobule and pyramis) receives input from several separate regions in the dorsal accessory nucleus, the medial accessory nucleus and portions of the principal nucleus. evidence is present for a topographical organization whereby specific regions of the olive project to restricted longitudinal zones. the visual-auditory region ... | 1978 | 641222 |
| an autoradiographic study of midbrain-diencephalic projections to the inferior olivary nucleus in the opossum (didelphis virginiana). | techniques of intra-axonal transport were utlizied to elucidate the organization of diencephalic and midbrain projections to the inferior olivary nucleus of the virginia opossum. retrograde transport of horseradish peroxidase injected into the olive suggests that terminals within it arise from the subparafascicular nucleus of the caudal thalamus, the nucleus of darkschewitsch, the fields of forel, the interstitial nucleus of cajal, the periaqueductal grey, the caudal pretectal nucleus, the tegme ... | 1978 | 641221 |
| ultrastructural localization of thermoplasma acidophilum surface carbohydrate by using concanavalin a. | surface carbohydrate, presumably the lipopolysaccharide, of thermoplasma acidophilum was visualized by means of the concanavalin a, horseradish peroxidase, and diaminobenzidine cytochemical staining procedure. | 1978 | 641014 |
| horseradish peroxidase application to the protein transport investigations and alkaline phosphatase ultralocalization at the kidney barrier level. | horseradish peroxidase was used to the precise investigation of low molecular protein transport across the kidney barrier. simultaneously on the parallel material alkaline phosphatase activity was revealed, as this enzyme is connected with the transport processes through cell membranes. the applied reaction diaminebenzidine has shown infiltration spots for low molecular protein at the glomercular barrier and visualized tubular reabsorption of peroxidase in proximal convoluted tubule. alkaline ph ... | 1978 | 640511 |
| a ventral lateral geniculate nucleus projection to the dorsal thalamus and the midbrain in the cat. | retrograde tracing experiments using horseradish peroxidase (hrp) have been utilized for demonstrating the origin of efferent projections of the ventral lateral geniculate nucleus (lgnv) in the cat. hrp-positive cells identifiable as origins of thalamic projections were found in lgnv after injections of hrp into the lateral central intralaminar nucleus. the labeled cells appeared concentrated in the medial part of the internal division of lgnv, consisting of medium-sized multipolar cells. contra ... | 1978 | 639913 |
| afferent fiber connections from lower brain stem to hypothalamus studied by the horseradish peroxidase method with special reference to noradrenaline innervation. | attempts were made to determine the afferent projections to the anterior hypothalamus including the preoptic area from the lower brain stem by means of the horseradish peroxidase method combined with monoamine oxidase staining to identify noradrenaline (na) neurons. in addition to this technique, a histofluorescence analysis was performed. na fibers in the medial part of the anterior hypothalamus were mainly supplied by a1 and a2 na neuron groups, while the lateral part and periventricular zone ... | 1978 | 639912 |
| projections of the ascending somesthetic pathways to the cat superior colliculus visualized by the horseradish peroxidase technique. | projections of the somesthetic ascending pathways to the superior colliculus (sc) of the cat have been demonstrated by means of the horseradish peroxidase (hrp) tracing technique. following hrp injection in one sc, marked neurons were found in the contralateral dorsal horn and in the contralateral dorsal column nuclei, mainly the cuneate nucleus. moreover, in the contralateral trigeminal complex, the sub-nucleus caudalis of the spinal nucleus of the vth nerve, and the subnucleus oralis plus the ... | 1978 | 639909 |
| uptake of horseradish peroxydase by the testis of locusta migratoria during the last larval instar; relation with variations of ecdysteroid levels in haemolymph. | by using horseradish peroxydase (hrp) as a tracer, it is shown that the gonial region of the locust testis is an "open" compartment which is almost always freely penetrated by the tracer. during the last larval instar, however, the penetration of hrp decreases and ceases at the time when high levels of ecdysteroids are detected in the haemolymph by radioimmunoassay. a cause and effect relationship between tracer uptake and hormonal level could not be demonstrated by the experiments carried out u ... | 1978 | 639097 |
| observations on the afferent and efferent connections of the avian isthmo-optic nucleus. | the efferent and afferent connections of the avian isthmo-optic nucleus (ion) were studied using light microscopic techniques. injections of [3h]proline into the nucleus resulted in labeling of centrifugal endings in the retina at the junction of the inner plexiform layer and inner nuclear layer, but produced no other transported label to any thalamic or mesencephalic nucleus. the origin of the tectal afferents to the ion was demonstrated by means of injections of [3h]proline into the most super ... | 1978 | 638787 |
| afferent projections to the central nucleus of the inferior colliculus in the rat. | the afferent projections to the inferior colliculus of the rat were studied using the method of retrograde transport of horseradish peroxidase (hrp). following large injections of hrp into the central nucleus, cells within the cochlear nuclei, superior olivary complex and auditory cortex were stained. within the contralateral dorsal cochlear nucleus, fusiform cells were heavily labeled. giant cells were also labeled in deeper layers. in the contralateral ventral cochlear nucleus, virtually all m ... | 1978 | 638786 |
| cells of origin of the spino-facial pathway in the cat: a horseradish peroxidase study. | 1978 | 638754 | |
| interaction of the hemin 2 and 4 substituents with apo horseradish peroxidase. | 1978 | 637861 | |
| increased permeability to horseradish peroxidase across cerebral vessels, evoked by electrically induced seizures in the rat. | under normal conditions a slight vesicular transfer of intravenously injected horseradish peroxidase (hrp) occurs across the endothelium of cerebral vessels, especially short segments of arterioles. the vesicular transport can be notably increased by chemically induced acute hypertension. in the present investigation 4 groups of animals received hrp, and the permeability of the cerebral endothelium was studied semimacroscopically, light microscopically and electron microscopically. the rats in g ... | 1978 | 636839 |
| neural spread of herpes simplex virus types 1 and 2 in mice after corneal or subcutaneous (footpad) inoculation. | twelve herpes simplex virus (hsv) strains, 6 of each type, were inoculated subcutaneously into the left hind foot and into the cornea of the right eye of 12-day-old swiss albino mice. the neural spread of virus to trigeminal and spinal ganglia and to brain and spinal cord was studied by demonstration of infective virus, histology and electron microscopy. type 1 and type 2 infections seemed to spread equally well by intra-axonal transport. using a protein tracer (horseradish peroxidase) injected ... | 1978 | 632838 |
| the organization of central auditory pathways in a reptile, iguana iguana. | the present experiments were designed to trace the central auditory pathways in an extant reptile, the new worlkd lizard--iguana iguana, utilizing anterograde axonal degeneration stained by the fink-heimer ('67) method and the retrograde axonal transport of horseradish peroxidase (lavail and lavail, '74). beginning with the projections of the auditory portion of the viiith nerve, the ascending pathways were traced through successive relay nuclei to the telencephalon. the auditory portion of the ... | 1978 | 632382 |
| association and commissural fiber systems of the olfactory cortex of the rat. | the association and commissural fiber systems arising in the olfactory cortical areas caudal to the olfactory peduncle (the piriform cortex, nucleus of the lateral olfactory tract, anterior cortical nucleus of the amygdala, periamygdaloid cortex and entorhinal cortex) have been studied utilizing horseradish peroxidase as both an anterograde and a retrograde axonal tracer. in the piriform cortex two sublaminae within layer ii (iia and iib) layer iii have been found to give rise to distinctly diff ... | 1978 | 632378 |
| afferent projections to the rat locus coeruleus as determined by a retrograde tracing technique. | afferent connections to the rat locus coeruleus (lc), which contains exclusively noradrenergic neurons, have been traced using the technique of retrograde transport of horseradish peroxidase (hrp). in order to ensure accurate placement of adequate amounts of hrp in the lc, a microiontophoretic delivery technique coupled with single cell recording was employed. the use of electro physiological "landmarks" as aids in placing the injections is described. following hrp injections into the lc, forebr ... | 1978 | 632368 |
| uptake of horseradish peroxidase from csf into the choroid plexus of the rat, with special reference to transepithelial transport. | protein uptake from cerebral ventricles into the epithelium of the choroid plexus, and transport across the epithelium were studied ultrastructurally in rats. horseradish peroxidase (hrp, mw 40,000) was used as protein tracer. steady-state ventriculo-cisternal perfusion with subatmospheric pressure (-10cm of water) in the ventricular system was applied. hrp dissolved in artificial csf was perfused from the lateral ventricles to cisterna magna for various times, and ventriculo-cisternal perfusion ... | 1978 | 630593 |
| visual corticopontine input to the paraflocculus: a combined autoradiographic and horseradish peroxidase study. | 1978 | 630397 | |
| postnatal development of rat cerebellum: massive and transient accumulation of concanavalin a binding glycoproteins in parallel fiber axolemma. | modifications of protein-bound sugars during postnatal development of rat cerebellum were studied. glycoprotein-bound mannose accumulates, in the particulate fractions, at an earlier age than the bulk of glycoprotein sugar. this corresponds to a transient and massive accumulation of glycoproteins which bind to concanavalin a (con a). these glycoproteins were localized by using fluorescent con a and the horseradish peroxidase-con a method. cerebellar white matter and the molecular layer bind mass ... | 1978 | 630386 |
| the location of spinal projection neurons in the cerebellar nuclei (cerebellospinal tract neurons) of the cat. a study with the horseradish peroxidase technique. | the distribution of spinal projection neurons was studied in the cerebellar nuclei of the cat following injections of horseradish peroxidase (hrp) into the cervical, thoracic and lumbar cord. hrp-positive (labeled) neurons were found in the medial (fastigial) and the posterior interpositus nuclei on the side contralateral to the cervical injection, being most numberous in cases with injections between the c2 and the c3 segments. in the medial nucleus (m) labeled neurons were distributed in the c ... | 1978 | 630384 |
| the vestibular nuclei in the domestic hen (gallus domesticus). iv. the projection to the spinal cord. | horseradish peroxidase was injected at various levels of the spinal cord of the hen and the cells in the brain stem, labeled due to retrograde transport of the tracer enzyme, were mapped with particular reference to the vestibular nuclear complex. the deiters' nuclei project ipsilaterally to the spinal cord. the projection from the nucleus deiters ventralis reaches down to the lumbosacral spinal cord. the spinal projection from the nucleus deiters dorsalis is somatotopically organized. cells loc ... | 1978 | 630381 |
| cortical projections of the thalamic mediodorsal nucleus in the rabbit. | the cortical projection of the thalamic mediodorsal nuclear complex (md) in the rabbit was mapped retrograde horseradish peroxidase and anterograde tritiated proline techniques. the projection field occupied the entire medial wall rostral to a mid corpus callosal level, wrapped around the frontal pole onto the lateral convexity and tailed off caudally on the dorsal bank of the rhinal sulcus. the projection of the lateral approximately one-half of md, the half which does not receive olfactory inp ... | 1978 | 626902 |
| the morphology of group ia afferent fibre collaterals in the spinal cord of the cat. | 1. the enzyme horseradish peroxidase (hrp) was injected into single ia muscle afferent fibres in anaesthetized cats. subsequent histochemistry allowed the morphology of the axons and their collaterals in the lumbosacral spinal cord to be determined. 2. fifteen ia axons were stained, four from medial gastrocnemius, four from lateral gastrocnemius-soleus and seven from muscles innervated by the posterior tibial nerve. all thirteen axons that could be traced into the dorsal roots bifurcated upon en ... | 1978 | 624988 |
| immunoelectron microscopic localization of lipopolysaccharides in the cell wall of bacteroides oralis and fusobacterium nucleatum. | lipopolysaccharides (lps) have been extracted and purified from two anaerobic gram-negative bacteria: bacteroides oralis and tfusobacterium nucleatum. chemical analysis of the preparations showed a great proportion of neutral sugars, mainly glucose, in lps of b. oralis. in rabbits, lps of b. oralis induced both immunoglobulin m and g antibodies in contrast to lps of f. nucleatum, to which only immunoglobulin m antibodies were produced. an immunohistochemical method with horseradish peroxidase-la ... | 1978 | 624589 |
| human marrow erythropoiesis in culture: iii. ultrastructural and cytochemical studies of cellular interactions. | human erythroblasts cultured with a methylcellulose clonal assay technique were studied with ultrastructural and cytochemical methods. the intact colonies contained only erythroblasts at a similar stage of maturation, and no macrophages were identified within the colonies. the cultured erythroblasts demonstrated many of the morphologic features described in vivo. neutral and acid glycoconjugates identified on the plasmalemma stained in a similar way to that seen in vivo with a concanavalin a hor ... | 1978 | 624337 |
| ionic strength dependence of the oxidation of iodide and ferrocyanide by compound i of horseradish peroxidase. | the kinetics of the oxidation of iodide and ferrocyanide by compound i of horseradish peroxidase have been studied at 25 degrees c as a function of ionic strength and ph. the ionic strength dependencies of the second-order rate constants are tested with an extended form of the debye-hückel equation for the activity coefficients of the reacting species. for the reaction of iodide with compound i it is shown that the ph variation of the rate constant at zero ionic strength is caused mainly by titr ... | 1978 | 624287 |
| early stages of uptake and transport of horseradish-peroxidase by cortical structures, and its use for the study of local neurons and their processes. | 1978 | 621289 | |
| uptake and fate of luminally administered horseradish peroxidase in resting and isoproterenol-stimulated rat parotid acinar cells. | the formation and fate of apical endocytic vesicles in resting and isoproterenol-stimulated rat parotid acinar cells were studied using luminally administered horseradish peroxidase (hrp) to mark the vesicles. the tracer was taken up from the lumen by endocytosis in small, smooth-surfaces "c"- or ring-shaped vesicles. about 1 h after hrp administration the vesicles could be found adjacent to the golgi apparatus. at later times hrp reaction product was localized in multivesicular bodies and lysos ... | 1978 | 618893 |
| a horseradish peroxidase study of the cerebellorubral pathway in the rat. | 1978 | 618740 | |
| occurrence of patulin in fruit and vegetables. | in different varieties of apples and pears suffering from brown rot, patulin was found in about 50 p. 100 of samples investigated (about 120). patulin levels as high as 1 g/kg rotten material were found 2-3 days after the fruit was removed from the cold store where it had been stored for 5 months. a significant diffusion into the healthy plant tissue was not observed in apples, but in peaches, tomatoes and pears. vegetable products with natural patulin content: apples, pears, peaches, apricots, ... | 1977 | 613910 |
| on the lysosomal function of juxtaglomerular granules. | the presence of acid phosphatase, beta-glucuronidase and aryl sulfatase in juxtaglomerular cell granules (jgg) as well as the uptake and concentration of certain low molecular weight dyes by these granules have repeatedly suggested that they are akin to lysosomes. in the present experiments, rats were injected with three substances of widely different molecular weight and physicochemical properties--sucrose, iron sorbitol-citric acid complex (jectofer) and horseradish peroxidase--that are well k ... | 1977 | 610707 |
| transmission of horseradish peroxidase across the small intestinal mucosa under tissue culture conditions. | 1977 | 610391 | |
| the cerebellar projection from the raphe nuclei in the cat as studied with the method of retrograde transport of horseradish peroxidase. | following injections of horseradish peroxidase (hrp) in the cerebellar cortex and nuclei of the cat, the distribution of labeled cells in the raphe nuclei was mapped. the findings confirm those made previously in studies of retrograde cell degeneration following cerebellar ablations (brodal et al., 1960a), and in addition reveal new details in the projection of the raphe nuclei onto the cerebellar cortex and nuclei. all the raphe nuclei except nucleus linearis intermedius and nucleus linearis ro ... | 1977 | 605998 |
| transendothelial vesicular transport of protein in brain edema induced by ultraviolet irradiation. | focal vasogenic brain edema was induced by ultraviolet irradiation of the exposed cerebral cortex of 16 cats. in 5 animals horseradish peroxidase was intravenously injected at times varying from 30 min to 24 h following irradiation and allowed to circulate for 45 min. fixation was carried out by perfusion and immersion with glutaraldehyde. the tissue, part of it incubated for peroxidatic activity, was treated for electron microscopy. the uv-irradiation leads to a shallow coagulation of the super ... | 1977 | 602683 |
| the reorganization of synaptic connexions in the rat submandibular ganglion during post-natal development. | 1. the innervation of neurones in the submandibular ganglion of neonatal and adult rats has been studied with intracellular recording, and light and electron microscopy. 2. intracellular recordings from neurones in isolated ganglia from adult animals showed that about 75% of the ganglion cells are innervated by a single preganglionic fibre. 3. however multiple steps in the post-synaptic potential (about five on average) were elicited in ganglion cells from neonatal animals by graded stimulation ... | 1977 | 599418 |
| topographical arrangement of thalamocortical neurons in the centrolateral nucleus (cl) of the cat, with special reference to a spino-thalamo-motor cortical path through the cl. | in the centrolateral nucleus of the thalamus (cl) in the cat, a topographical arrangement of the thalamocortical projection neurons was demonstrated by utilizing retrograde axonal transport of horseradish peroxidase (hrp). following injections of hrp into the medial or lateral areas of the anterior sigmoid gyrus (asg), hrp-labeled neurons were located medially or laterally in the caudal levels of the cl, respectively; neurons in the central areas of the cl were labeled after injections of hrp in ... | 1977 | 598437 |
| [development of arrangement and degradation of the system of rough endoplasmic reticulum in antibody-producing plasma cells]. | during the immune response of the cells of murine omentum to horseradish peroxidase which had been administered intraperitoneally together together with endotoxin or endotoxoid acting as an adjuvans the plasma cells showed several different patterns of the rough endoplasmic reticulum [rer] system. irregular to bizarre rer structures occurred plasma cells during the initial phase of the immune reaction only, whereas the plasma cells found at later stages had characteristic highly organized rer sy ... | 1977 | 597922 |
| localization of adductor and abductor motor nerve fibers to the larynx. | knowledge of the location of motor nerve fibers to the adductor and abductor muscles of the larynx may be useful in the diagnosis and treatment of innervation disorders in this organ. anterograde degeneration and retrograde tracer anatomical techniques have demonstrated the central and peripheral positions of these two groups of motor nerve fibers in the cat. traditional nerve fibers degeneration methods applied following intracranial transection of the vagus nerve rootlets indicated that: 1) mo ... | 1977 | 596775 |
| movement of horseradish peroxidase in submandibular glands of dogs after ductal injection. | small volumes of solutions of a tracer enzyme, horseradish peroxidase (hrp), have been injected retrogradely via the main duct of submandibular glands in dogs, and the distribution of the peroxidase reaction product within the glands has been studied light microscopically and electron microscopically at different times after the injection. tissue taken immediately after injections of 0.25 ml, given over 10 sec, showed that hrp had penetrated into the interstitial spaces of the gland. it was usua ... | 1977 | 592901 |
| the morphology of hair follicle afferent fibre collaterals in the spinal cord of the cat. | 1. the enzyme horseradish peroxidase (hrp) was injected into single axons that innervated hair follicle receptors to study the morphology of their collaterals in the dorsal horn of the cord. the axons were impaled near the dorsal root entrance zone in the lumbosacral spinal cord of anaesthetized cats and hrp injected by passing current through the intra-axonal micro-electrode. the morphology was revealed by subsequent histochemistry.2. thirteen hair-follicle afferent fibres were stained includin ... | 1977 | 592215 |
| a study on the postulated transoptic regulation of the intraocular pressure. | the possibility that fibers regulating the intraocular pressure originate in the hypothalamus and course in the optic nerve, has been investigated in albino rabbits with different techniques. by means of horseradish perixodase tracing-technique, oculohypothalamic but no hypothalamoocular connections were observed. the water-loading effect on the intraocular pressure was studied after unilateral optic nerve transection. the transection was performed in three experimental groups in the following w ... | 1978 | 580841 |
| bulbospinal projections to the intermediolateral cell column: a neuroanatomical study. | the location of those neurones in the brain stem that project to the intermediolateral column (ilc) from which preganglionic sympathetic neurones have their origin was studied by the method of retrograde transport of horseradish peroxidase (hrp). in cats 30--50 nl of a 30% hrp solution was injected into the region of the ilc at t3 or l1 on one side. after a survival period of 72 h the lower brain stem from c1 to the inferior colliculi was sectioned and prepared for histological study under brigh ... | 1979 | 575994 |
| morphologic and functional changes of the aortic intima during experimental hypertension. | the morphology and permeability to horseradish peroxidase of the rat aortic intima have been investigated in three experimental models of hypertension having different values of plasma renin content and plasma aldosterone level. during hypertension the aortic endothelium shows three main changes: 1) increased arithmetic mean thickness, with prominent rough endoplasmic reticulum and polyribosomes; 2) the appearance of actin microfilament bundles; and 3) increased permeability to horseradish perox ... | 1979 | 573070 |
| barriers and transport properties of the perineurium. an ultrastructural study with 125i-labeled albuminin and horseradish peroxidase in normal and protein-deprived rats. | the permeability properties of the perineurium in sciatic nerves of 12-week-old rats were studied. the penetration of 125i-labeled albumin and horseradish peroxidase into the perineurium was investigated electronmicroscopically 10, 30, and 120 min after the local extraneural application of the tracers. the autoradiographic study included age-matched protein-deprived rats. it was concluded that the perineurium acted as a diffusion barrier but also permitted a slow passage of the macromolecules in ... | 1979 | 573043 |
| horseradish peroxidase/hydrogen peroxide-catalyzed binding of aromatic hydrocarbons to dna. | 1979 | 572364 | |
| granule secretion by the luteal cell of the sheep: the fate of the granule membrane. | the ultrastructure of the corpus luteum of the sheep has been examined at the mid-stage of the estrous cycle when progesterone secretion is active. secretory granules are associated with the secretion of this hormone, and the evidence indicates that the granule membrane becomes incorporated into the plasma membrane during exocytosis. further evidence of this process has been obtained from studies on the uptake of horseradish peroxidase by the luteal cells. | 1979 | 572262 |
| blood-brain barrier dysfunction to peroxidase after air embolism, aggravated by acute ethanol intoxication. | ethanol-intoxicated and non-intoxicated rats were injected with horseradish peroxidase and evan's blue-labelled albumin and given a small air embolus in the right common carotid artery after ligation of the external carotid branch. in both ethanol-intoxicated and non-intoxicated rats, some endothelial cells, mainly in arteries and arterioles, showed a diffuse distribution of peroxidase in the cytoplasm. in some arterioles with a diffuse endothelial distribution of peroxidase there was a detachme ... | 1979 | 571899 |
| afferent projections related to attack sites in the pontine tegmentum. | attack by a cat on a rat was elicited by electrical stimulation of the ventral parabrachial nucleus or the paralemniscal pontine reticular formation. the attack was quiet and it differed from similar behavior elicited at other sites in that the cat not only bit the rat but struck it with its paw. horseradish peroxidase was deposited at the attack sites. cells sending projections to the attack sites were located in gyrus proreus, anterior, central, and medial amygdala, the bed nucleus of the stri ... | 1979 | 570870 |
| hardening of the sea urchin fertilization envelope by peroxidase-catalyzed phenolic coupling of tyrosines. | within minutes after its elevation from the egg surface, the sea urchin fertilization envelope (fe) becomes "hardened" by a reaction that renders it resistant to agents that solubilize, denature or degrade most proteins. peroxidase activity is released into the surrounding seawater from stronglyocentrotus purpuratus eggs during fertilization. evidence from several sources indicate that the catalytic action of the peroxidase is responsible for hardening the fe through the phenolic coupling of tyr ... | 1978 | 569021 |
| proton nuclear magnetic resonance spectra of compounds i and ii of horseradish peroxidase. | enzymatic reaction intermediates of horseradish peroxidase, compounds i and ii, were studied by high-resolution nuclear magnetic resonance spectroscopy at 220 mhz. the heme peripheral proton peaks were successfully obtained in the downfield region of 50 to 80 ppm from 4,4-dimethyl-4-silapentane-5-sulfonate for compound i and of 10 to 20 ppm for compound ii at ph 9.2. this indicates that no isoporphyrin appears in the catalytic cycle of the enzyme. temperature dependences of the spectra also were ... | 1978 | 568936 |
| neurones within the "chemosensitive area" on the ventral surface of the brainstem which project to the intermediolateral column. | with the method of retrograde transport of horseradish peroxidase it has been demonstrated that neurones within the "chemosensitive area" of the brainstem project to the thoracic intermediolateral column. the function of these neurones is discussed in regard to the regulation of blood pressure. | 1978 | 567798 |
| localization of concanavalin a binding sites on the cell membrane of herpetomonas samuelpessoai: influence of growth conditions. | herpetomonas samuelpessoai agglutinates when incubated with concanavalin a (con a). agglutination involved binding of con a to specific receptors as it was inhibited by alpha-methyl-d-mannoside. the pattern and the intensity of the agglutination as well as the localization of con a-binding sites were influenced by the composition of the growth medium, growth temperature and time of incubation. con a-binding sites were detected by using the con a-horseradish peroxidase-diaminobenzidine technique. | 1978 | 567601 |
| ultrastructural studies on blood-brain barrier dysfunction around cerebral stab wounds, aggravated by acute ethanol intoxication. | ethanol-intoxicated and non-intoxicated albino rats subjected to cerebral stab wounds were injected with evans blue-labelled albumin (eba) and horseradish peroxidase (hrp) 24 hours after the injury. the tracers were injected 30 and 2 minutes before perfusion fixation with glutaraldehyde and then prepared for light microscopy and electron microscopy. there was prominent leakage of peroxidase through chains of pinocytotic vesicles and transendothelial channels from blood, through the endothelial c ... | 1978 | 566999 |
| effect of daily ethanol ingestion on intestinal permeability to macromolecules. | the effects of regular ethyl alcohol ingestion on morphological and permeability characteristics of the small intestine were assessed in mature rats using the tracer protein, horseradish peroxidase. thirty adult rats were divided into two groups and provided a standard commercial diet in pellet form. each morning, after an overnight fast, every animal in the experimental group was administered by gavage an aliquot of 20% ethanol; animals in the control group were provided aliquots of 20% sucrose ... | 1978 | 563673 |
| septal afferents to the arcuate-median eminence region in the guinea pig: correlative electrophysiological and horseradish peroxidase studies. | 1977 | 562702 | |
| concanavalin a-binding by cells of the early chick embryo. | the surfaces of cells from the early embryo of the chick were examined using electron microscope techniques for the visualization of concanavalin a-binding sites. horseradish peroxidase and ferritin labelled concanavalin a were used to determine the distribution of the binding sites. all surfaces of the epiblast and hypoblast layers which were accessible to concanavalin a showed the presence of binding sites in stage 1 embryos. the ventral surface of the epiblast showed a high lectin affinity wh ... | 1977 | 561795 |
| kinetics of antibody and immunoglobulin-producing cells appearing in popliteal lymph nodes of mice stimulated with horseradish peroxidase. | 1977 | 561126 | |
| experimental spinal cord compression by epidural neoplasm. | we have developed an experimental model of spinal cord compression in rats. tumor injected anterior to the t-12 vertebral body grows through the intervertebral foramina to compress the cord and produces paraplegia in 3 to 4 weeks. evidence for vasogenic edema in spinal cord compressed by tumor includes increased water content, leakage of horseradish peroxidase into gray matter, and histologic evidence of edema. the vascular supply to the cord overlying the tumor appears to be compromised. both s ... | 1977 | 558545 |
| [regulation of antibody secretion: observation made on cultured lymphoid cells from immune rabbit lymph nodes]. | rabbits were immunized with soluble and particulate antigens (horseradish peroxidase or srbc) in complete freund's adjuvant. four to twelve weeks after footpad immunization, popliteal lymph nodes were removed and the lymphoid cells were cultured with autologous serum for 5 to 7 days. using local hemolysis assay, we observed a recrudescence of antibody secreting cells when the autologous serum present in the medium had previously been freed of antibody by specific adsorption. this phenomenon was ... | 1977 | 557948 |
| induction of pinocytosis in rat hepatocytes by partial hepatectomy. | rat hepatocytes, normally not highly pinocytic cells, becomes so after partial hepatectomy when about two-thirds of the liver is removed. droplets, up to 20 mum in diameter, develop, initially by addition to smaller pinocytic structures and later by fusion with lysosomes. the droplets contain a material with an electron microscope periodicity characteristic of fibrin; they are periodic acid schiff-positive as is plasma. it is therefore reasonable to consider plasma glycoproteins to be major comp ... | 1977 | 557041 |
| [motoneural organisation of the anterior horn with respect to the peripheral nerves in the rat (as studied by the method of retrograde transport of horseradish peroxidase)(author's transl)]. | the motoneuronal organisation of the anterior horn with respect to the peripheral nerves, is still imprecise due to methodological problems. the chromatolytic and golgi methods have furnished some fruitful but incomplete data. the retrograde transport of horseradish peroxidase permits to obtain both the topography of cells and their dendritic tree. with such a method we studied the cervical enlargment of the rat's spinal cord. each nerve is in relation with a column of motoneurons whose both the ... | 1979 | 555020 |
| labelled trigeminal ganglion cells after injection of horseradish peroxidase in the extraocular muscles and iiird nerve of the lamb. | after injection of hrp in the oculomotor nerve labelled cells were found in the dorsal most part of the ophthalmo-maxillary area of the semilunar ganglion. below this area a labelled cellular pool was detected following the injection of the enzyme in the extrinsic eye muscles. a clear somatotopic arrangement of these muscles was noticeable. | 1979 | 553536 |
| cells of origin of motor axons in the subdiaphragmatic vagus of the rat. | the central cell groups that give rise to the motor axons that travel in the subdiaphragmatic vagus were re-examined in the rat by transecting the dorsal or ventral vagus near the stomach and incubating the nerve stump in crystalline horseradish peroxidase (hrp). an exceedingly large percentage of cells was labeled throughout the dorsal motor nucleus of the vagus (mx), with labeled cells extending even beyond the rostro-caudal limits of the nucleus usually assigned on the basis of cytoarchitectu ... | 1979 | 553094 |
| electrophysiological and neuroanatomical studies of hepatic portal osmo- and sodium-receptive afferent projections within the brain. | previous investigations concerning the function of hepatic sodium and osmoreceptors indicated that the activation of these chemoreceptive structures significantly affected physiological and behavioral mechanisms related to water and electrolyte homeostasis. though anatomical studies predicted that such information should follow the previously described gustatory afferent pathy, until recently, the precise course taken by higher order hepatic afferents was unknown. the studies described herein ve ... | 1979 | 553093 |
| afferent and efferent connections of the area postrema demonstrated by the horseradish peroxidase method. | the previous autoradiographic study of the efferent connections of the arca postrema have been completed by using horseradish peroxidase (h.r.p.) as a marking agent. these two labelling techniques have made it possible to determine two categories of structures connected with a.p.: those with afferent connections (nucleus ambiguus), or efferent connections (mesencephalic nucleus of v, locus coeruleus, griseum centrale and inferior and superior colliculi) and those having both types of connections ... | 1979 | 550738 |
| hydrophobic binding of phenoxyacetic and phenylacetic acids to horseradish peroxidase and human serum albumin: structure-activity relationships. | studies of protein binding in homologous series of drugs are of great interest for drug research. apparent binding constants of phenoxyacetic and phenylacetic acids to horseradish peroxidase and to human serum albumin are evaluated by nmr studies and an optical method. these constants are good parameters to describe hydrophobic interactions, and the results are in a good agreement with our protein binding model described previously. | 1979 | 549409 |
| thalamoamygdaloid connections studied by the method of retrograde transport. | on the basis of retrograde horseradish peroxidase transport from nuclei ot the amygdaloid body of the rat to the thalamus, it was found that several groups of thalamic nuclei send fibers to the amygdala. these are: (i) nuclei of posterior region of thalamus and neighboring area of the tegmentum - peripeduncular nucleus, suprageniculate-limitans nucleus, (ii) midline nuclei - paraventricular nucleus, parataenial nucleus, nucleus reuniens, (ii) intralaminar nuclei - central medial nucleus, parafas ... | 1979 | 547711 |
| nigral projections to the inferior and the superior colliculus in the rat: a horseradish peroxidase study. | 1979 | 547209 | |
| [partial purification of hog thyroid peroxidase using detergent treatment and its spectral changes induced by hydrogen peroxide (author's transl)]. | thyroid peroxidase (tpo) was partially purified from hog thyroid microsomes after solubilization by means of deoxycholate treatment followed by ammonium sulfate farctionation and affinity chromatography with con a sepharose. the absorption spectra of the preparation showed the maxima at around 410 nm for oxidized form, 422 nm for dithionite-reduced form and 422 nm for co complex of reduced form. the cyanide difference spectrum showed a peak at 431 nm and a trough at 403 nm. this preparation was ... | 1979 | 544287 |
| motor nuclear representation of masticatory muscles in the rat. | representation of the masticatory muscles within the motor trigeminal nucleus was studied in rats by the horseradish peroxidase (hrp) method and the antidromic field potential method. the motor trigeminal nucleus of the rat could be divided cytoarchitecturally into a dorsolateral and a ventromedial division. within the dorsolateral division, the temporal muscle was represented dorsomedially, the masseter muscle dorsolaterally and laterally, and the lateral and medial pterygoid muscles ventrolat ... | 1979 | 541900 |
| the relationship between some measures of synaptic ultrastructure as a function of distance from the soma on lamprey reticulospinal neurons. | synaptic junctions located on the dendrites of lamprey (petromyzon marinus) reticulospinal neurons labelled with intracellularly-injected horseradish peroxidase were studied. the normal ultrastructure of the synaptic junctions was defined and several quantitative measures made from each junction in order to test the hypothesis that distally-located synapses are ultrastructurally different from those located at proximal dendritic sites. a total of 820 contacts from one neuron and 279 from a secon ... | 1979 | 541691 |
| the blood-nerve barrier and reconstitution of the perineurium following nerve grafting. | sural nerve autografts were performed on intact rat sural nerves and on sural nerves excised proximal to the site of grafting. the effect of the presence or absence of regenerating axons upon reconstitution of the perineurium at the graft junctions and upon re-establishment of the blood-nerve barrier to horseradish peroxidase (hrp) were studied over the succeeding 3--24 weeks. compartmentation of the nerve fascicle occurred at the graft junctions where the perineurium was damaged. each compartme ... | 1979 | 537675 |
| localization of synaptic input on dendrites of a lamprey spinal cord neurone from physiological measurements of membrane properties. | 1. composite excitatory post-synaptic potentials (e.p.s.p.s) resulting from electrotonic and chemical synaptic junctions were recorded from eighteen interneurones following stimulation of the i2 burster axon in the isolated lamprey spinal cord. 2. in each cell, the half-width of the electrotonic e.p.s.p. was measured and used, together with the cable parameters estimated for the same neurone, to locate the position of synaptic contact made by the i2 axon on the dendrites of the interneurone. the ... | 1979 | 536916 |
| the origin of reticulospinal fibers in the rat: a hrp study. | the distribution as well as morphological characteristics of brain stem reticular neurons projecting to spinal cord both of aminergic and non-aminergic natures in the rat was investigated by means of the retrograde horseradish peroxidase (hrp) method. for the identification of aminergic neurons, a combination of hrp technique and monoamine-oxidase staining as well as a pretreatment of 6-hydroxydopamine and 5,6-dihydroxytryptamine (5,6-dht) was applied. following the injection of hrp to the cervi ... | 1979 | 536593 |
| the regions of the fornix longus: a morphologic analysis. | the areas surrounding the fornix longus were examined using the rapid golgi method, acetylcholinesterase (ache) histochemistry, and horseradish peroxidase histochemistry. parasagittal golgi impregnated sections revealed the neuronal configurations of the nuclei of the fornix longus. an interstitial nucleus within the fiber bundle and a bed nucleus were observed. they coursed from midline dorsal hippocampus and became continuous with caudal septal regions (notably septotriangularis). neurons in t ... | 1979 | 536586 |