Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [a new immunochemical method for detecting substance p receptors based on the biotin-streptavidin system]. | a novel method for detecting the membrane receptors of substance p (sp) has been developed. the method does not require radioactive derivatives of sp and is based on quantitation of specifically bound biotinylated sp (bt-sp), the analysis being performed after destruction of the ligand-receptor complex in acidic conditions and bt-sp extraction into solution. the acidic extract from the bt-sp-membrane complex after neutralization is added to immulon microelisa plate coated with affinity purified ... | 2005 | 1280142 |
| highly sensitive sandwich enzyme immunoassay for alpha-fetoprotein in human saliva. | to determine alpha-fetoprotein (afp) in human saliva, a highly sensitive sandwich enzyme immunoassay for saliva afp was developed. afp standards and saliva samples were added into the wells of a polystyrene plate coated with goat igg antibody against human afp. after incubation, the wells were washed and horseradish peroxidase-labelled antibody was added. the enzyme activity specifically bound to the well was assayed using 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide as substrate. the re ... | 1992 | 1280027 |
| antibody-antigen complex formation with immobilized immunoglobulins. | we have investigated the complex formation between an immobilized monoclonal antibody and antigens that differ in size about 50-fold. as a model system, we used an iodinated progesterone derivative and a progesterone-horseradish peroxidase conjugate as tracer and a monoclonal antibody as binding protein. the antibody was immobilized by four different methods: physical adsorption, chemical binding, and binding via protein g in the absence or presence of a protective protein (gelatin). these inves ... | 1992 | 1280003 |
| long-range afferents in the rat spinal cord. ii. arborizations that penetrate grey matter. | 1. the caudal extent of the collateral arborizations of entering sensory fibres in rat spinal cord was investigated by two methods: bulk labelling of peripheral nerves by injection of horseradish peroxidase conjugated to cholera toxin (b-hrp) and by antidromic stimulation using small currents from microelectrodes in the spinal cord while recording from single units in peripheral nerve or dorsal root. 2. the results show that injection of b-hrp into the sural or sciatic nerve labelled sural affer ... | 1992 | 1279734 |
| peripheral injury and anterograde transport of wheat germ agglutinin-horse radish peroxidase to the spinal cord. | previous observations have revealed labeling in the extracellular space surrounding boutons and unmyelinated fibers in superficial laminae of the spinal cord after injection of the tracer wheat germ agglutinin conjugated to horseradish peroxidase in dorsal root ganglia. the degree of extracellular labeling appeared related to the extent of the damage to the ganglia at the time of the injection. to determine whether injury might produce extracellular labeling, we investigated the effects of unila ... | 1992 | 1279465 |
| topographical organization of the nigrotectal projection in rat: evidence for segregated channels. | recent evidence suggests that projections from the superior colliculus to the brainstem in rat are organized into a series of anatomically segregated output channels. to understand how collicular function may be modified by the basal ganglia it is important to know whether particular output modules of the superior colliculus can be selectively influenced by input from substantia nigra. the purpose of the present study was, therefore, to examine in more detail topography within the nigrotectal sy ... | 1992 | 1279464 |
| synaptic organization of gabaergic inputs from the striatum and the globus pallidus onto neurons in the substantia nigra and retrorubral field which project to the medullary reticular formation. | anatomical tract-tracing and immunohistochemical techniques involving correlated light and electron microscopy were used to determine whether the descending striatal and pallidal afferents to the substantia nigra pars reticulata converge onto individual neurons projecting to the pontomedullary and medullary reticular formation in the rat. injections of biocytin into the ventrolateral region of the striatum and phaseolus vulgaris-leucoagglutinin into the ventrolateral and caudal regions of the gl ... | 1992 | 1279463 |
| afferent connections of the parvocellular reticular formation: a horseradish peroxidase study in the rat. | the afferent connections of the parvocellular reticular formation were systematically investigated in the rat with the aid of retrograde and anterograde horseradish peroxidase tracer techniques. the results indicate that the parvocellular reticular formation receives its main input from several territories of the cerebral cortex (namely the first motor, primary somatosensory and granular insular areas), districts of the reticular formation (including its contralateral counterpart, the intermedia ... | 1992 | 1279462 |
| evidence that the masticatory muscles receive a direct innervation from cell group k in the rabbit. | these experiments have shown that a group of neurons lateral to the trigeminal motor nucleus innervates the muscles of mastication. the work began to describe the location of digastric last-order interneurons, using the technique of transneuronal labeling with wheatgerm agglutinin-conjugated horseradish peroxide injected into the left digastric muscle of rabbits under general anaesthesia. four to eight days later, the animals were killed with an overdose of anaesthetic and perfused. coronal sect ... | 1992 | 1279458 |
| calbindin d28k-containing nonpyramidal cells in the rat hippocampus: their immunoreactivity for gaba and projection to the medial septum. | calbindin d28k-containing non-pyramidal cells were found in all layers and subfields of the hippocampus, with the highest frequency in stratum radiatum of the ca1-ca3 subfields. a large number of these neurons had a vertically oriented dendritic tree, often restricted to to stratum radiatum. in stratum oriens and near to the border of strata radiatum and lacunosum moleculare cells with horizontally running dendrites were also found. multipolar cells were most common in stratum radiatum of the ca ... | 1992 | 1279455 |
| pathways of macromolecular tracer transport across venules and small veins. structural basis for the hyperpermeability of tumor blood vessels. | blood vessels supplying tumors are hyperpermeable to macromolecules, but the mechanisms responsible are poorly understood. | 1992 | 1279271 |
| electrophysiology of the mammillary complex in vitro. i. tuberomammillary and lateral mammillary neurons. | 1. the electrophysiological properties of the tuberomammillary and lateral mammillary neurons in the guinea pig mammillary body were studied using an in vitro brain slice preparation. 2. tuberomammillary (n = 79) neurons were recorded mainly ventral to the lateral mammillary body as well as ventromedially to the fornix within the rostral part of the medial mammillary nucleus. intracellular staining with horseradish peroxidase (n = 9) and lucifer yellow (n = 3) revealed that these cells have seve ... | 1992 | 1279134 |
| regeneration of adult rat cns axons into peripheral nerve autografts: ultrastructural studies of the early stages of axonal sprouting and regenerative axonal growth. | if one end of a segment of peripheral nerve is inserted into the brain or spinal cord, neuronal perikarya in the vicinity of the graft tip can be labelled with retrogradely transported tracers applied to the distal end of the graft several weeks later, showing that cns axons can regenerate into and along such grafts. we have used transmission em to examine some of the cellular responses that underlie this regenerative phenomenon, particularly its early stages. segments of autologous peroneal or ... | 1992 | 1279130 |
| cooperative binding of concanavalin a to thymocytes at 4 degrees c and micro-redistribution of concanavalin a receptors. | the mode of binding of 125i-labelled concanavalin a and succinyl-concanavalin a to rat thymocytes at 4 degrees c was investigated. simultaneously, the free binding sites of the cell-bound lectin molecules were quantified by horseradish peroxidase binding. concanavalin a showed cooperative binding while succinyl-concanavalin a did not. the number of molecules of concanavalin a bound to the cell surface when it was saturated was twice the number of molecules of succinyl-concanavalin a. we interpre ... | 1976 | 1278183 |
| ultrastructure and permeability of lymph node microvasculature in the mouse. | the microvasculature of lymph nodes and peyer's patches consists of arterioles, capillaries and venules. the postcapillary segment comprises high-endothelial venules (he venules) as well as ordinary venules. in order to study the ultrastructure of the microvasculature, particularly with respect to the nature of intercellular junctions, lanthanum and ruthenium red were used as tracers. furthermore, to evaluate the permeability properties of the different segments of the microvasculature, intraven ... | 1976 | 1277282 |
| changes in the distribution of the dentate gyrus associational system following unilateral or bilateral entorhinal lesions in the adult rat. | the distribution of the dentate gyrus associational system was analyzed in naive adult rats and in those with either unilateral or bilateral lesions of the entorhinal cortex. horseradish peroxidase histochemistry was used to trace the origin and course of this intrinsic fiber system. the fibers originated in the ca3-4 pyramidal cell field, apparently medial to the origin of the schaffer collateral system, and followed a trajectory which was essentially identical to that described for this system ... | 1976 | 1276951 |
| monosynaptic inputs to caudate neurons identified by intracellular injection of horseradish peroxidase. | 1976 | 1276937 | |
| trans-glial channel-facilitated translocation of tracer protein across ventral nerve root sheaths of crayfish. | trans-glial channels, which traverse the multilamellate glial sheath of crayfish nerves, are easily recognized in freeze-fracture preparations. their structure and position in the glial layers of the sheath strongly supports the suggestion that they serve to facilitate rapid movement of molecules and fluids from outside the sheath to the surface of axons contained within. segments of ventral ganglion nerve roots, which were ligated at their free ends, were immersed in crayfish ringer solution co ... | 1976 | 1276892 |
| striatal afferent connections in the turtle (chrysemys picta) as revealed by retrograde axonal transport of horseradish peroxidase. | horseradish peroxidase (hrp, 30% solution, 0.1-0.3 mul, 72 h) was injected unilaterally into the basal striatum (str) and the dorsal ventricular ridge (dvr) of adult turtles (chrysemys picta) in order to demonstrate the cells of origin of some afferents to these telencephalic structures. after selective str injection, hrp-labeled cells were visualized in the dorsal thalamus and midbrain tegmentum, ipsilaterally. at thalamic level, hrp-positive neurons were located around nucleus rotundus, i.e., ... | 1976 | 1276890 |
| an improved technique for the microinjection of horseradish peroxidase. | 1976 | 1276885 | |
| direct projections from hypothalamus to hippocampus in the rat demonstrated by retrograde transport of horseradish peroxidase. | 1976 | 1276883 | |
| infrared spectroscopic studies of carbonyl horseradish peroxidases. | infrared difference spectra, feiiico vs. feiii of horseradish peroxidase isoenzymes a2 and c were recorded from 2000 to 1800 cm-1. under alkaline conditions, ph 9, both isoenzymes exhibit two co stretching bands, at 1938 and 1925 cm-1 for a2 and at 1933 and 1929 cm-1 for c. as the ph is lowered the low-frequency band for each isoenzyme decreases in intensity with a concommitant appearance and increase in intensity of a band at 1906 and 1905 cm-1 for the a2 and c isoenzymes, respectively. these c ... | 1976 | 1276134 |
| a study of the supposed hydroxylation of tyrosine catalysed by peroxidase. | the claim that peroxidase (rather than tyrosinase) is the enzyme responsible for the conversion of tyrosine into dopa (3,4-dihydroxyphenylalanine) in melanogenesis was investigated. the spectral changes that occurred during the action of horseradish peroxidase in the presence of h2o2 on dopa, tyrosine and mixtures of dopa with tyrosine or other phenolic compounds were studied. the effect of ascorbic acid or dihydroxyfumaric acid on some of these changes was also investigated. no evidence was fou ... | 1976 | 1275895 |
| endocytosis by vascular smooth muscle cells in vivo and in vitro. roles or vesicles and lysosomes. | overloading of lysosomes of smooth muscle cells with excess substrate may be a key event in the development of hypertensive and atherosclerotic vascular disease. cellular uptake of materials and its relation to lysosomal function were studied by ultrastructural cytochemistry in aortic smooth muscle cells grown in vitro and in the intact animal. injection of horseradish peroxidase (hrp) into hypertensive rats resulted in rapid insudation of the material into the environs of medial smooth muscle c ... | 1976 | 1275059 |
| permeability alteration of sarcolemmal membrane in catecholamine-induced cardiac muscle cell injury. in vivo studies with fine structural diffusion tracer horse radish peroxidase. | cardiac muscle cell injury was produced in male sprague-dawley rats weighing 300 to 450 gm. with catecholamines, norepinephrine, and isoproterenol; sarcolemmal membrane alteration was tested in vivo using the extracellular macromolecular tracer, horseradish peroxidase. norepinephrine was administered in continuous intravenous infusion in a dose of 4 to 6 mug. per 100 gm. of body weight per minute, whereas isoproterenol was given as a single subcutaneous injection in a dose of 8.5 mg. per 100 gm. ... | 1976 | 1271749 |
| development of an enzyme immunoassay for human placental lactogen using labelled antibodies. | an immunoassay for the determination of human placental lactogen (hpl) has been developed using specific antibodies labelled with horseradish peroxidase. a method is presented in which the enzyme : antibody conjugate is purified with an antigenic solid phase to ensure the production of conjugates with intact immunological activity. serum components interfere with the assay but this problem can only be avoided at the expense of a precise and practical assay for the determination of hpl. | 1976 | 1270811 |
| organization of the tectofugal visual pathway in the pigeon: a retrograde transport study. | in birds, superficial laminae of the optic tectum receive a massive retinal input; the tectum in turn projects upon the nucleus rotundus thalami, which then sends its efferents to the ectostriatal core of the telencephalon. to examine the detailed organization of this principal ascending visual pathway, small injections of the marker horseradish peroxidase (hrp) were placed in various sites throughout the ectostriatum (e) or nucleus rotundus (rt) in pigeons. analysis of the resulting patterns of ... | 1976 | 1270632 |
| descending pathways from the brain stem to the spinal cord in some reptiles. i. origin. | in the present study of the origin of the pathways descending from the brain stem to the spinal cord has been investigated in the reptiles testudo hermanni, pseudemys scripta elegans, tupinambis nigropunctatus and python reticulatus. these reptiles, using highly different types of progression, have been selected, because fundamental variations in the organization of the central motor apparatus are to be expected. the origin of the descending pathways has been demonstrated by recording the occurr ... | 1976 | 1270628 |
| scanning electron microscopy of the subarachnoid space in the dog. iv. subarachnoid macrophages. | young dogs of both sexes were used in this study. transmission and scanning electron microscopy were utilized for the examination of the spinal cord and choroid plexus with emphasis on the study of free cells. these procedures were modified so that, in certain cases, the same cells observed in scanning electron microscopy could be analyzed internally by transmission electron microscopy. one half of the animals were injected under anesthesia with horseradish peroxidase for observation of phagocyt ... | 1976 | 1270624 |
| trans-glial channels in ventral nerve roots of crayfish. | the sheath around the roots of the sixth abdominal ganglion in the ventral nerve cord of the crayfish consists of concentric layers of thin glial processes alternating with wide clefts containing filamentous connective tissue. regions of each glial lamella are perforated by single, short, tubular channels: the trans-glial channels. in thin plastic sections examined in the electron microscope, the channels appear as slits that are 240 a wide and 450-550 a long which traverse glial lamellae less t ... | 1976 | 1270620 |
| the olivocerebellar projection in the cat as studied with the method of retrograde axonal transport of horseradish peroxidase. ii. the projection to the uvula. | following injections of small quantities of horseradish peroxidase (hrp) suspension in the uvula of the cat, the distribution of labeled cells in the inferior olive has been mapped. the findings confirm the conclusion made on the basis of studies of retrograde cell loss in the olive following ablations of the uvula (brodal, '40b) that two small olivary subdivisions, the nucleus beta and the dorsomedial cell column project heavily to the uvula. in addition the hrp-study shows that the uvula recei ... | 1976 | 1270615 |
| convergent thalamic and mesencephalic projections to the anterior medial cortex in the rat. | small microelectrophoretic deposits of horseradish-peroxidase (hrp) were placed at various loci within the gray matter of the rat's anterior medial cortex. a comparison of the patterns of retrograde cell-labeling charted in 26 such cases confirmed earlier findings in fiber-degeneration studies according to which the respective thalamocortical projections of the mediodorsal (md) and anteromedial nucleus (am) overlap each other over a wide region of the anterior medial cortex. this region of thala ... | 1976 | 1270614 |
| projections of the locus coeruleus and adjacent pontine tegmentum in the cat. | the projections of the locus coeruleus and adjacent pontine tegmentum have been studied using anatomical and physiological methods in the cat. axonal trajectories were traced using either the fink-heimer i method following electrolytic lesions, or the autoradiographic method after injection of tritiated proline into the nucleus. results with both methods were similar. axons of locus noeruleus neurons ascended ipsilaterally through the mesencephalon lateral to the medial longitudinal fasiculus, v ... | 1976 | 1270609 |
| the structure of lymphatic capillaries in lymph formation. | the lymphatic vascular system consists of endothelial lined vessels which begin as blind-end tubes or saccules that are located within the connective tissue areas. this system serves as a one-way drainage apparatus for the removal of diffusible substances as well as plasma proteins that escape the blood capillaries. if permitted to accumulate, these escaped components would deplete the circulatory system of its plasma colloids and disrupt the balance of forces responsible for the control of flui ... | 1976 | 1269772 |
| nmr studies of hemoproteins: ph dependence of ferric horseradish peroxidase and horse heart myoglobin. | 1976 | 1269749 | |
| immunohistochemical localization of lh-producing cells in the adenohypophysis of the japanese quail, coturnix coturnix japonica. | the cells that produce luteinizing hormone (lh) in the adenohypophysis of the japanese quail were identified immunohistochemically using anti-chicken lh serum and horseradish peroxidase-labeled goat anti-rabbit gamma globulin serum. the lh cells are localized in the caudal lobe of the pars distalis. they are elongate in shape shape and are polarized toward the sinusoids, especially in their active states. alterations in size of lh cells are directly related to changes in circulating lh levels as ... | 1976 | 1268921 |
| the extravascular channel system in the rostral pituitary of mugil cephalus (teleostei) as revealed by use of horseradish peroxidase. | the interlobular spaces, separating the neuro- and the adenohypophysis can be visualized in the pituitary of the grey mullet by using the horseradish peroxidase technique. on the one hand, these spaces communicate with the pericapillary spaces. on the other, they are linked with the pericellular spaces in the adenohypophysis and the periaxonal spaces in the neurohypophysis, thus providing a pathway for the exchange of metabolites and hormones between blood system, epithelial cells and the termin ... | 1976 | 1268912 |
| magnetic circular dichroism studies on horseradish peroxidase. | magnetic circular dichroism (mcd) spectra were observed for native (fe(iii)) horseradish peroxidase (peroxidase, ec 1.11.1.7), its alkaline form and fluoro- and cyano-derivatives, and also for reduced (fe(ii)) horseradish peroxidase and its carbonmonoxy-- and cyano- derivatives. mcd spectra were obtained for the cyano derivative of fe(iii) horseradish peroxidase, and reduced horseradish peroxidase and its carbonmonoxy- derivative nearly identical with those for the respective myoglobin derivativ ... | 1976 | 1268223 |
| the isolation and characterization of the glycopeptides from horseradish peroxidase isoenzyme c. | the purity of horseradish peroxidase isoenzyme c was demonstrated using isoelectric focusing, polyacrylamide gel electrophoresis at two ph values and cellulose acetate electrophoresis at two ph values. the glycopeptides obtained upon trypsin digestion were isolated using the plant lectin, concanavalin a, and were resolved using paper electrophoresis. the carbohydrate content of the native peroxidase was 86% accounted for by the carbohydrate content of the glycopeptides thus suggesting little los ... | 1976 | 1268213 |
| acute hypersensitivity pneumonitis in the rabbit. i. an animal model with horseradish peroxidase as antigen. | an experimental model of acute hypersensitivity pneumonitis was developed in rabbits using horseradish peroxidase as antigen. the lesion, produced by aerosol challenge with horseradish peroxidase 21 days after a single parenteral immunization with horseradish peroxidase and complete freund adjuvant, was characterized by diffuse infiltration of respiratory bronchioles and of alveolar ducts and walls with approxomately equal numbers of mononuclear cells (lymphocytes and macrophages) and polymorph ... | 1976 | 1267257 |
| transendothelial vesicular transport of protein following compression injury to the spinal cord. | routes of vascular leakage resulting in trauma-induced edema have not been clarified. to explore the problem we followed the fate of intravascular horseradish peroxidase (hrp) after compression injury to the thoracic cord (cats). at 90 seconds and 15 minutes, hrp was confined to the gray matter, occupying perivascular spaces, unexpanded extracellular channels, and cytoplasmic compartments of injured cells. adjoining segments contained similar but lesser deposits. at 4 hours, tracer occupied the ... | 1976 | 1263445 |
| glomerular permeability to proteins. effects of hemodynamic factors on the distribution of endogenous immunoglobulin g and exogenous catalase in the rat glomerulus. | the distribution of endogenous immunoglobulin g (igg) and exogenous catalase was delineated in the rat glomerulus under normal and abnormal hemodynamic conditions. igg was identified by an ultrastructural immunoperoxidase technique using antirat igg fab fragments conjugated to horseradish peroxidase; catalase was identified by a cytochemical reaction. when superficial glomeruli in anesthetized munich-wistar rats were rapidly fixed in situ by dripping glutaraldehyde onto the renal surface, igg an ... | 1976 | 1263444 |
| afferents to the cerebellar lateral nucleus. evidence from retrograde transport of horseradish peroxidase after pressure injections through micropipettes. | hrp was injected by pressure from glass capillary micropipettes unilaterally into the lateral nucleus of rat so as to encompass the entire nucleus, but without spread into the interpositus nuclei. the cells of origin of the afferents to the lateral nucleus were studied after retrograde transport of the hrp. the reticulotegmental nucleus of the pons was labelled bilaterally and is the major source of crossed and uncrossed reticular imputs. the pontine nuclei also provide extensive crossed and unc ... | 1976 | 1262557 |
| a somatotopic and functional representation of the respiratory muscles in the trigeminal and facial motor nuclei of the carp (cyprinus carpio l.). | the place of the cells innervating six most important respiratory muscles in the carp (cyprinus carpio l.) were determined by means of the horseradish peroxidase technique. it was found that these muscles are represented in the rostral and caudal subdivisions of the trigeminal and facial motor nuclei. all connections were ipsilateral. the distribution of the various cell populations over the different subnuclei has been related to the function of the respective muscles in the respiratory cycle. ... | 1976 | 1262554 |
| gustatory pathways in the bullhead catfish. 1. connections of the anterior ganglion. | the central projections of the external gustatory system in bullhead catfish were examined using orthograde degeneration and retrograde transport of horseradish peroxidase (hrp) techniques. both large and small cells were observed in the anterior ganglion which contains a mixture of elements from the trigeminal, facial and anterior lateral line nerves. some of the large cells on the lateral margin of the ganglion were found to belong to the lateral line system. no separation of trigeminal and fa ... | 1976 | 1262542 |
| ultrastructural studies of mouse leucocytes showing surface binding of peroxidase-conjugated lectins. | examination in the electron microscope of mouse spleen cells treated with conjugates of wheatgerm agglutinin and horseradish peroxidase has shown the surface of myeloid cells to be heavily stained. resting lymphocytes were unstained but became positive after stimulation by phytohaemagglutinin or concanavalin a, suggesting a relationship between the presence of this surface component and the functional state of the cell and providing a further point of similarity between myeloid and lymphoblastoi ... | 1976 | 1261094 |
| horseradish peroxidase/hydrogen peroxide-catalyzed oxidation of the carcinogen n-hydroxy-n-acetyl-2-aminofluorene as effected by cyanide and ascorbate. | horseradish peroxidase and h2o2 mediate n-hydroxy-n-acetyl-2-aminofluorene (n-oh-aaf) conversion into two more potent carcinogens, 2-nitrosofluorene and n-acetoxy-n-acetyl-2-aminofluorene. optical studies of this system indicate that horseradish peroxidase is operating as a peroxidase with n-oh-aaf as the electron donor. our studies confirm the earlier finding that 2-nitrosofluorene and n-acetoxy-n-acetyl-2-aminofluorene are the products of the type ii enzyme-mediated oxidation of n-oh-aaf, but ... | 1976 | 1260768 |
| intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spinocervical tract cells. | 1976 | 1260464 | |
| effects of 2,4-substituents of deuteroheme upon the stability of the oxy-form and compound i of horseradish peoxidases. | 1976 | 1259443 | |
| the porphyrin-sensitized photooxidation of horseradish apoperoxidase. | 1976 | 1259421 | |
| simultaneous visualization by light microscopy of two pituitary hormones in a single tissue section using a combination of indirect immunohistochemical methods. | two indirect methods involving enzyme-labeled antibodies were used to demonstrate simultaneously two distinct tissue antigens in the same histologic section without a need for antigen-antibody dissociative procedures. sections of rat pituitary gland were incubated with rabbit anti-rat luteinizing hormone followed by goat anti-rabbit gamma-globulin conjugated to horseradish peroxidase. the same sections were then further incubated with monkey anti-rat growth hormone followed by goat anti-monkey g ... | 1976 | 1254941 |
| cerebello-olivary fibers: their origin, course and distribution in the north american opossum. | although degeneration techniques suggest that cerebello-olivary fibers are limited in their origin and distribution, horseradish peroxidase and autoradiographic experiments make it clear that they arise within all cerebellar nuclei and project to most, if not all, areas of the contralateral inferior olive. autoradiographic preparations show that cerebello-olivary fibers are highly ordered and suggest that the dentate nucleus projects primarily to the principal olive, the interpositus anterior re ... | 1976 | 1253856 |
| [experience with the use of horseradish peroxidase for light and electron microscope studies of interneuronal connections]. | by the method based on a retrograde axonal transport of exogenous horseradish peroxidase (hrp), the origins of afferentation of the motor cortex of adult cats, kittens and albino rats were studied. hrp-positive neurons were found by light and electron microscopy in the somatosensory cortex (c1) of the ipsilateral hemisphere and in the portions of the cortex of the contralateral hemisphere which were symmetrical to the site of injection of hrp. the disposition of neurons, marked by hrp, in the vt ... | 1976 | 1252130 |
| the penetration of fluorescein-conjugated and electrondense tracer proteins into xenopus tadpole optic nerves following perineural injection. | the permeability of xenopus tadpole optic nerves to macromolecules was studied in order to evaluate the usefulness of this system for studying mechanisms of serum-induced cns demyelination in vivo. single injections of either horseradish peroxidase (hrp), ferritin or fluorescein-conjugated human igg were injected around the right optic nerve and tadpoles were then sacrificed between 15 min and 48 h. each of the tracers had penetrated the nerve parenchyma by 30 min. entry of hrp and ferritin occu ... | 1976 | 1247883 |
| peroxidase activity of alveolar macrophages. | peroxidase activity was studied in alveolar macrophages and compared to the peroxidase activity in polymorphonuclear leukocytes using cytochemical techniques. a dense reaction product for peroxidase was observed in the primary lysosomes of polymorphonuclear leukocytes, but no significant peroxidase or peroxidative enzymes could be detected in rabbit alveolar macrophages. furthermore, following vigorous phagocytosis of zymosan particles by alveolar macrophages in vitro, no peroxidase could be det ... | 1976 | 1246123 |
| organization of thalamocortical auditory system in the cat studied with horseradish peroxidase. | 1976 | 1244977 | |
| electron microscopic studies on the blood-aqueous barrier of prostaglandin-treated rabbit eyes. ii. iris. | the distribution of intravenously injected horseradish peroxidase in the iris of prostaglandin (e1 and e2)-treated rabbit eyes, has been studied with the electron microscope. peroxidase was demonstrated in the stroma of all parts of the iris. the tracer was found throughout interendothelial clefts of iris vessels, indicating that these vessels had become permeable to peroxidase. the distribution of peroxidase in the iris epithelium indicates that the posteroir epithelial cells are girdled by zon ... | 1975 | 1242595 |
| electron microscopic studies on the blood-aqueous barrier of prostaglandin-treated rabbit eyes. i. iridial and ciliary processes. | prostaglandins e1 and e2 were applied topically to rabbit eyes. structures related to the blood-aqueous barrier in the iridial and ciliary processes, as well as the permeability of the ciliary epithelium to the protein tracer horseradish peroxidase, were studied with the electron microscope. marked morphological changes, including dilations of the intercellular spaces and separation of the two epithelial cell layers, were found in the epithelium of the iridial processes. only minor structural ch ... | 1975 | 1242594 |
| in vitro studies on peroxidase movement in the epithelium of prostaglandin-treated rabbit ciliary bodies. | the movement of horseradish peroxidase (hrp) in the epithelium of isolated rabbit iris/ciliary body preparations, has been studied with the electron microscope. hrp was applied at the stromal side of the epithelium, and was left for 60 and 120 min. the distribution pattern of hrp found in the epithelium of the iridial and ciliary processes is consistent with in vivo studies, i.e. the progression of hrp is blocked at the site of the zonula occludens of the superficial epithelium. the hrp distribu ... | 1975 | 1242593 |
| [exogenous peroxidase in the eggs of rats during cleavage]. | the presence of exogenous peroxidase of the rat's eggs during cleavage at the 1-, 2-, 8-cellular stages and the blastocysts were studied after 30 minute incubation with horseradish peroxidase (molecular weight 40.000, approximate size of the molecule 40 a). the activity of exogenous peroxidase in the area of zona pellucida was established in the perivitelline space, in the cytoplasm - in the pinocytosis vesicles, in certain greater vacuoles and in certain secondary lysosomes. at the stage of bla ... | 1975 | 1241658 |
| dorsal column nuclei projections to the cerebellar cortex in cats as revealed by the use of the retrograde transport of horseradish peroxidase. | the existence of a cerebellar projection from the dorsal column nuclei (gracile and cuneate nuclei, dcn) has been proposed on electrophysiological grounds but questioned when studied with neuroanatomical techniques. the retrograde transport of horseradish peroxidase (hrp) has been used for the present study and provides anatomical evidence of a dcn-cerebellar pathway. in adult cats, 1 to 6 mul of 30% hrp were injected in pars intermedia of the anterior lobe (lobules iv-v), in paramedial lobule a ... | 1975 | 1236873 |
| absorption of horseradish peroxidase by the small intestinal epithelium in postnatal developing rats. | after an intraluminal injection of horseradish peroxidase into the small intestine, the localization of peroxidase was studied in neonatal developing and adult rats by means of electron microscopy. until around the 14th day of the neonatal period absorbed peroxidase granules in the duodenal and jejunal epithelium were abundant in the microvillous membrane, the apical tubulo-vacuolar system, and the golgi apparatus, and on the lateral cell and basal membranes, and the luminal surfaces of the capi ... | 1975 | 1234392 |
| blood-brain barrier dysfunction in acute lead encephalopathy: a reappraisal. | acute lead encephalopathy was induced in adult guinea pigs by administering daily oral doses of lead carbonate. during the development of the encephalopathy, the structural and functional integrity of the blood-brain barrier was evaluated with electron microscopy and tracer probes. blood, cerebral gray matter, liver, and kidney were analyzed for lead, calcium, and magnesium content. the animals regularly developed an encephalopathy after four doses of lead. there were no discernible pathomorphol ... | 1975 | 1227864 |
| [flavonols and flavones of vegetables. v. flavonols and flavones of root vegetables (author's transl)]. | root vegetables contain flavon(ol) glycosides in tracers up to small amounts, while the level of their leaves are in part considerable (to more than 1 g/kg, calculated as aglycon). radish, rutabagas, scorzoneras, and beets contain less than 1 mg/kg kaempferol and/or quercetin; carrots less than 1 mg/kg apigenin and luteolin; celery roots ca. 75 mg apigenin/kg and 14 mg luteolin/kg; horseradish about 20 mg kaempferol/kg and small radish 1-10 mg kaempferol/kg, whereby all these flavones and flavon ... | 1975 | 1224798 |
| electron microscopic study on permeability of coronary artery wall of normotensive rabbits using horseradish peroxidase as a tracer. | in order to study the pathways in the coronary artery vessel wall horseradish peroxidase (hrp) was used as a tracer. hrp was injected directly in the left ventricle of rabbit hearts. 30 seconds after intracardial injection of hrp in normotensive rabbits, peroxidase reaction products could be located in random areas of the coronary arterial system and could then be observed in all three layers of the coronary walls at these locations. hrp reaches the subendothelial space of the coronary arteries ... | 1975 | 1220671 |
| rabbit homocytotropic antibody response to horseradish peroxidase: the role of contaminating antigens. | 1975 | 1218893 | |
| some enzymes present in the walls of mesophyll cells of tobacco leaves. | cell-wall enzymes were assayed by the difference between enzyme activities in the whole cell and the protoplast. both peroxidase (85.2%) and acid phosphatase (21.9%) were located in the wall. however, malate dehydrogenase was found only in the protoplast. a study of the time-course of the release of peroxidase and malate dehydrogenase into the incubation medium from cells either treated with cellulase or untreated, also indicated that peroxidase and not malate dehydrogenase was located in the wa ... | 1975 | 1212211 |
| acute lead encephalopathy in the guinea pig. | acute lead encephalopathy was induced in adult guinea pigs with daily oral doses of lead carbonate. cerebral capillaries were examined by electron microscopy, and the blood-brain barrier (b-bb) evaluated with evans blue and horseradish peroxidase. brain lead levels were also determined during the developing encephalopathy. there was no cerebral capillary alteration or demonstrable b-bb dysfunction. brain lead concentrations increased over the 5-day period. the encephalopathy in the absence of an ... | 1975 | 1211109 |
| contractile apparatus in aortic endothelium of hypertensive rat. | one week after hypertension was produced in male wistar rats (150 g body weight) by a complete aortic ligature placed between renal arteries, electron microscopic studies showed a striking increase of cytoplasmic microfilaments in the endothelial cells of the aortic segment above coarctation (mean blood pressure 160 mm hg). these microfilaments measured 40-70 a in diameter and were located particularly close to the endothelial clefts. in "en face" preparation of aortic endothelial cells treated ... | 1975 | 1209006 |
| functional and structural changes in mammalian sympathetic neurones following interruption of their axons. | the effects of interrupting the axons of principal neurones in the superior cervical ganglion of adult guinea-pigs were studied by means of intracellular recording, and light and electron microscopy. 1. within 72 hr of axon interruption, the amplitude of exitatory postsynaptic potentials potentials (e.p.s.p.s) recorded in principal neurons in response to maximal preganglionic stimulation declined. e.p.s.p.s were maximally reduced (by more than 70% on average) 4-7 days following interruption, and ... | 1975 | 1206535 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. | the distribution of labeled cells in the inferior olive of the cat has been mapped following injections of small amounts of horseradish perosidase in the paramedian lobule of the cerebellum. the distribution of labeled cells was plotted in drawings of approximately serial transverse sections. the findings in each case were transferred to a standard diagram of the olive to facilitate comparison of cases. previous studies of the distribution of retrograde cell loss in the inferior olive following ... | 1975 | 1206129 |
| olfactory relationships of the telencephalon and diencephalon in the rabbit. ii. an autoradiographic and horseradish peroxidase study of the efferent connections of the anterior olfactory nucleus. | the efferent connections of the anterior olfactory nucleus in the female albino rabbit have been studied using the autoradiographic and horseradish peroxidase methods for tracing axonal pathways. following a unilateral injection of 3h-leucine into the olfactory peduncle, radioactively labeled efferent projections from the anterior olfactory nucleus were traced into all layers of the ipsilateral main olfactory bulb beneath the olfactory nerve layer and through the ipsilateral anterior limb of the ... | 1975 | 1206126 |
| proceedings: spectroscopic and nmr studies on the binding of substrates to horseradish peroxidase. | 1975 | 1205735 | |
| proceedings: modifications of horseradish peroxidase with aromatic compounds. | 1975 | 1205733 | |
| [evidence for direct projections from the hypothalamus to the lateral geniculate and occipital cortex of the cat as demonstrated by horseradish peroxidase technique (author's transl)]. | 1975 | 1204798 | |
| an ultrastructural study of normal and injured hypoglossal nuclei after injection of horseradish peroxidase. | the left hypoglossal nuclei of normal rats, and rats which had received left hypoglossal nerve axotomies 7-21 days previously, were studied by electron microscopy after injection of horseradish peroxidase as a marker of extracellular space and pinocytosis. quantitative analysis showed that the number of pinocytotically-derived structures in presynaptic boutons was significantly increased in rats at 7, 14, and 21 days after axotomy when compared with normal rats. it was suggested that presynaptic ... | 1975 | 1204692 |
| structural changes in the membrane of vero cells infected with a paramyxovirus. | vero cells productively infected with the halle strain of measles virus have been studied by means of surface replication, freeze-fracturing, and surface labeling with horseradish peroxidase-measles antibody conjugate in order to examine changes in the structure of the cell membrane during viral maturation. early in infection, the surfaces of infected cells are embossed by scattered groups of twisted strands, and diffuse patches of label for viral antigens cover regions marked by these strands. ... | 1975 | 1202014 |
| anti-horseradish peroxidase associated with tubular structures in the endoplasmic reticulum of plasma cells in stimulated lymph nodes of mice. | tubular structures are described in dilated cisternae of the endoplasmic reticulum in plasma cells of lymph nodes stimulated with horseradish peroxidase (hrp). a positive reaction for anti-hrp antibody suggests that they are formed of immunoglobulins or are at least closely associated with them. | 1975 | 1199967 |
| changes in vascular permeability in stroke-prone spontaneously hypertensive rats studied with peroxidase as a tracer. | cerebrovascular permeability in stroke-prone spontaneously hypertensive rats (shr) at various ages was histologically studied using horseradish peroxidase as a tracer and such was related to the cerebrovascular lesions in the animals. an increase in permeability was demonstrated in the brain of shr, particularly in those animals with an extremely high blood pressure. increased cerebrovascular permeability occurred in some animals without any organic vascular change or severe parenchymal changes, ... | 1975 | 1199727 |
| [comparison of the activities of free and carrier fixed horseradish peroxidase]. | commerical horseradish-peroxidase was covalently bound to brcn-activated sepharose. the activity parameters vmax and km were determined by the leukomalachite green reaction. compared with the soluble enzyme, the immobilized pod has a relative residual activity of 22%. dimethylsulfoxide and formamide were found to diminish the enzymatic activity in a concentration-dependent manner. the activity of the free enzyme in aqueous formamide solution (10%) is reduced by 43%, that of the insolubilized enz ... | 1975 | 1199630 |
| membrane permeability alterations as manifestation of early cardiac muscle cell injury. | studies with extracellular fine structural diffusion protein tracers, horseradish peroxidase and ferritin, were carried out on catecholamine-induced cardiac muscle cell injury. epinephrine, norepinephrine, and isoproterenol were administered in a dose of 4- 6 mug/100g body weight by continuous intravenous infusion. for follow-up studies, isoproterenol was also given subcutaneously in a dose of 8.5 mg/100 g body weight as a single injection. in contrast to saline-infused controls and following ep ... | 1975 | 1197896 |
| altered reabsorption of protein by the renal cortex in rats treated with hypertonic saline or mannitol. | the reabsorption of horseradish peroxidase (hrp) by the proximal tubule cells of rat kidneys was investigated by measuring the concentration of hrp in total particulate fractions of the cortex 1/4 and 1 hr after intravenous injection, and by correlated cytochemical observations. when compared to the corresponding values of the control animals, the concentration of hrp 1 hr after injection was decreased approximately 10-fold in the renal cortex of rats which had received an intravenous injection ... | 1975 | 1194661 |
| localization of the efferent neurons of the tensor tympani muscle of the newborn kitten using horseradish peroxidase. | 1975 | 1193198 | |
| cell populations of the lateral geniculate nucleus of the cat as determined with horseradish peroxidase. | 1975 | 1192198 | |
| the central cervical nucleus as cell origin of a spinocerebellar tract arising from the cervical cord: a study in the cat using horseradish peroxidase. | 1975 | 1192184 | |
| the haem-enviroment in horseradish peroxidase as seen by proton magnetic relaxation. | 1975 | 1191283 | |
| location of cat trigeminal ganglion cells innervating dental pulp of upper and lower canines studied by retrograde transport of horseradish peroxidase. | 1975 | 1188971 | |
| proceedings: a quantitative study of horseradish peroxidase uptake by boutons presynaptic to axotomized hypoglossal neurones. | 1975 | 1185647 | |
| vasogenic edema in the injured spinal cord: a method of evaluating the extent of blood-brain barrier alteration to horseradish peroxidase. | 1975 | 1183533 | |
| the correlation of the time of origin of neurons with their axonal projection: the combined use of [3h]thymidine autoradiography and horseradish peroxidase histochemistry. | 1975 | 1182549 | |
| the retrograde transport of horseradish peroxidase from the developing limb of the chick embryo. | chick embryos ranging in age from 4.0 to 18 days of incubation and 1-2-day-old hatchlings received injections of hrp solutions directly into the leg musculature. after survival periods of from 5 to 25 h motoneurons and cells in the spinal sensory ganglia were found to be stained with the hrp reaction product. it was found that the first appearance of a positive hrp reaction coincided with the time when nerve processes are first detected in the limb-bud by silver techniques (i.e. at 4.5 days of i ... | 1975 | 1182520 |
| the origin of the annulo-olivary tract with notes on other mesencephalo-olivary pathways. a study by the horseradish peroxidase method. | 1975 | 1182508 | |
| mössbauer investigations of high-spin ferrous heme proteins. ii. chloroperoxidase, horseradish peroxidase, and hemoglobin. | reduced samples of chloroperoxidase, horseradish peroxidase, and deoxyhemoglobin were studied by mössbauer spectroscopy in strong magnetic fields. the intricate paramagnetic spectra of chloroperoxidase were evaluated in detail in the framework of a spin hamiltonian pertinent to high-spin ferrous iron. the studies strongly suggest that, in their reduced states, chloroperoxidase from caldariomyces fumago and cytochrome p-450 from pseudomonas putida have similar, if not identical ligand structures ... | 1975 | 1182095 |
| mössbauer investigations of high-spin ferrous heme proteins. i. cytochrome p-450. | anaerobically reduced samples of cytochrome p-450 from pseudomonas putida were studied by mössbauer spectroscopy. in the presence of an applied magnetic field the high-spin ferrous heme iron showed an intricate pattern of electric and magnetic hyperfine interactions which could be parametrized successfully in terms of a spin hamiltonian formalism. the results imply a very low (triclinic) symmetry of the heme iron. the effects of the ligand environment and of spin-orbit coupling result in a large ... | 1975 | 1182094 |
| ultrastructural studies on cerebrovascular permeability in acute hypertension. | acute hypertension, experimentally induced by intravenous injection of metaraminol in adult rabbits, rapidly induced a damage of the blood-brain barrier in the cerebral cortex, as visualized by evans-blue-conjugated albumin and horseradish peroxidase. extravasation of these two exogenous tracers was demonstrated to occur in arterioles, in capillaries and, rarely, in venules. peroxidase passed the endothelial cell into the nervous tissue in either or three different ways, i.e. through channels, o ... | 1975 | 1180001 |
| localization of masticatory motoneurons in the cat and rat by means of retrograde axonal transport of horseradish peroxidase. | topographical localization of monotoneurons supplying the masticatory muscles was investigated in the cat and rat, utilizing retrograde axonal transport of horseradish peroxidase. following injection of horseradish peroxidase in each masticatory muscle, motoneurons labelled with peroxidase were seen to be aggregated into a cluster within the motor nucleus of the trigeminal nerve. such clusters of peroxidase-motoneurons innervating each masticatory muscle were demarcated more sharply in kittens t ... | 1975 | 1176649 |
| different targets of different neurons in nucleus gracilis of the cat. | efferent projections of neurons in the cat nucleus gracilis to the ventrobasal complex (vb) of the thalamus and the dorsal accessory portion of the inferior olive (iod) were studied using tracing techniques that exploit neuronal orthograde and retrograde intra-axonal transport processes. these projections were studied in order to determine if the heterogeneity of the morphology, physiology and afferent input of the nucleus gracilis is paralleled by a similar heterogeneity in its efferent project ... | 1975 | 1176641 |
| circulation and turnover of synaptic vesicle membrane in cultured fetal mammalian spinal cord neurons. | intact neurons in cultures of fetal rodent spinal cord explants show stimulation-dependent uptake of horseradish peroxidase (hrp) into many small vesicles and occasional tubules and multivesicular bodies (mvb) at presynaptic terminals. presynaptic terminals were allowed to take up hrp during 1 h of strychnine-enhanced stimulation of synaptic transmitter release and then "chased" in tracer-free medium either with strychnine or with 10 mm mg++ which depresses transmitter release. tracer-containing ... | 1975 | 1176531 |
| similarities and differences of five peroxidases from turnip and horseradish. peptide mapping studies on glycoproteins. | four isoperoxidases of turnip root and isoperoxidase c of horseradish root were digested with trypsin, and their peptide maps, prepared by high-voltage paper electrophoresis, were compared. all five tryptic digests were completely soluble at ph 8. the maps were developed with a variety of general and specific reagents: ninhydrin, histidine, tyrosine, tryptophan and arginine reagents. cystine peptides and cysteic acid derivatives have also been characterized. all detected half-cystine residues se ... | 1975 | 1175650 |
| delineation of dorsal lateral geniculate afferents from the cat brain stem as visualized by the horseradish peroxidase technique. | 1975 | 1174981 |