Publications

cloning and nucleotide sequence determination of a heat-stable enterotoxin gene from vibrio cholerae non-o1.		1990	2101148
characterization of vibrio cholerae non-o1 hemagglutinin/protease.		1990	2101140
[derivative spectroscopy and its use in sanitary hygiene control].	an analytical method is developed for defining the residual quantities of rodenticide "brodifacoum" (talon) used in the control of harmful field rodents and especially for species microtus. the method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. the second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. the peak height in this interval is in proportio ...	2007	2099444
[the effect of immune antibodies and the xanthine oxidase-xanthine enzymatic link on vibrio cholerae].	as revealed in experiments on v. cholerae, highly diluted cholera antiserum enhanced the inhibitory action of the enzymatic link xanthine oxidase-xanthine-fe2+ on the multiplication of v. cholerae, while low dilutions of the antiserum weakened this action. normal rabbit serum produced no such effect. the antivibrionic effectiveness of the immune molecular cycle, viz. antiserum--the xanthine oxidase enzymatic link, was found to depend also on the concentration of xanthine. immune antibodies to ch ...	1990	2099072
[the determination of the optimal inoculation dose of an oral cholera chemical bivalent vaccine in a controlled experiment].	276 volunteers aged 19 years and over were placed under observation in the course of the trial of oral cholera vaccine in tablets, containing choleragen toxoid, o-antigens of serovars inaba and ogawa and a number of vibrio cholerae exoenzymes, for safety, reactogenic properties and immunological effectiveness. the vaccine was found to produce no reactions in a dose of 1-4 tablets; the administration of 3 tablets (300,000 binding units of the toxoid and 10,000 units of o-antigens, serovars inaba ...	1990	2099071
the occurrence of alpha (1----2) linked n-acetylperosamine--homopolymer in lipopolysaccharides of non-o1 vibrio cholerae possessing an antigenic factor in common with o1 v. cholerae.	chemical analysis was carried out on lipopolysaccharides from vibrio cholerae bio-serogroup hakata 487-85. the o-specific chain of the phenol-soluble lipopolysaccharides was demonstrated by 13c-nmr spectroscopy and methylation analysis to contain a linear homopolymer of alpha(1----2) linked n-acetylperosamine (4-acetamido-4,6-dideoxy-d-mannopyranose), which was closely similar to but not identical to a linear alpha(1----2) linked n-3-deoxy-l-glycerotetronyl (s-2,4-dihydroxybutyryl) perosamine-ho ...	1990	2098633
epidemiological profile of outbreaks of cholera in india during 1975-1989.	cholera has been present in india since antiquity. six pandemics originated in indian subcontinent. the present seventh pandemic caused by el tor vibrio cholerae started from indonesia (sulawesi) in 1961 and entered india in 1964. by the end of 1965 it has replaced the age old classical v. cholerae. many of the states which never had cholera or were free from it for a long time got infected and became endemic foci of el tor infection. this article reviews the epidemiological features of importan ...	1990	2098418
[degenerative encephalopathy of probable viral etiology in the course of congenital agammaglobulinemia].		2007	2096763
[cognition, memory and affect].	the flow of information within the brain and the sequence of operations which process it get enriched through a twofold mediation, that of the current affective states and that of the references made to an individual life-history. affectivity and memory are closely linked in the elaboration of action (thought acts, speech acts, behaviours) since it is especially through the mediation of affective states and emotions that the current action is both a partial reactualization of the past and one of ...	2007	2096709
spinal cord repair: is tissue oxygenation an important variable?	we have demonstrated the reliability and feasibility of making pto2 recordings from graft and host tissue in the injured spinal cord. the data suggest that the oxygen microenvironment of developing graft and host spinal tissue is clearly different from that found in normal spinal tissue or in transplants that have not survived or integrated well. these same constraints seem to apply to cavitation in developing grafts and poorly developed graft/host interfaces. the similarity between these findin ...	1990	2096642
[use of the "sartorius" cutaneous absorption simulator for transdermal pharmaceutical forms of nsaids].	we report the results of the in vitro-release of the active ingredients (flurbiprofen and ibuprofen) from dermal form obtained by the sartorius absorption simulator sm16750. the results show that the choice of the excipients is basic. using nsaid, the aqueous gel can be considered the suitable formulation to obtained a good in vitro-release. the quantities of the active ingredients released during the time are plotted in diagrams.	2007	2095811
["the profession is not the cause of the crisis"].		2006	2095089
[experiences in incidence monitoring and control of the pharaoh ant in a health resort].	today monomorium pharaonis still represents a threat of the hygiene regime in the objects of public health and welfare, particularly for the asepsis and the "black-white-separation". the experiences collected throughout a successful rub out by the preparate "lafarex n 87" in a health-resort are presented. the necessity of cooperation of all concerned persons (owners, pest control enterprises and public hygiene service) are emphasized.	2005	2095048
[aminoacyl-trna synthetases (codases) and their noncanonical functions].	the aim of this review is to summarize the data obtained in the author's laboratory during the last decade. the main objects of these investigations were mammalian aminoacyl-trna synthetases, mainly bovine tryptophanyl-trna synthetase (ec 6.1.1.2). the data are discussed and compared with those described in literature. in the course of these studies it turned out that some properties of mammalian aminoacyl-trna synthetases for instance, nuclear location of some of the synthetases, presence of ex ...	1990	2094804
the mechanism of inhibition of s-adenosyl-l-homocysteine hydrolase by fluorine-containing adenosine analogs.	(z)-4',5'-didehydro-5'-deoxy-5'-fluoroadenosine (i), 5'-deoxy-5'-difluoroadenosine (ii), and 4',5'-didehydro-5'-deoxy-5'-fluoroarabinosyl-adenosine (iii) are inhibitors of rat liver s-adenosyl-l-homocysteine hydrolase. compounds i and ii are time-dependent and irreversible inhibitors of the enzyme. both i and ii are oxidized by e.nad to produce e.nadh, and fluoride anion is formed in the inactivation reaction (0.7 to 1.0 mole fluoride/mole of enzyme subunit, and 1.7 moles fluoride/mole of enzyme ...	1990	2094766
the morphology of synovial lining of various structures in several species as observed with scanning electron microscopy.	data concerning surface morphology of synovial lining of tendons, tendon sheaths, cruciate ligaments, infra-patellar fat pads and peripatellar synovial ridges in various species (rat, rabbit, dwarf goat, sheep, pig, dog, human) are reported on. supportive studies with transmission electron microscopy (tem) and light microscopy were performed. three principal morphological appearances of the synovium are evident. on structures with a dense fibrous architecture like tendons, tendon sheaths and cru ...	1990	2094014
immunogenicity of liposome-associated oral cholera vaccine prepared from combined vibrio cholerae antigens.	liposomes were prepared from bovine brain sphingomyelin and cholesterol. they were reinforced by incorporation of osmium tetroxide to prevent their immediate degradation inside the host. combined vibrio cholerae antigens (lipopolysaccharide, crude cell-bound hemagglutinin and procholeragenoid) were orally administered to experimental rats either as free or liposome-associated. a total of 70 experimental rats was utilized in experiments comparing the immune responses of rats to liposome-associate ...	1990	2091663
randomized double-blind placebo controlled trial to evaluate the safety and immunogenicity of the live oral cholera vaccine strain cvd 103-hgr in swiss adults.	a randomized, double-blind, placebo controlled trial was conducted in 50 healthy swiss adults to assess the safety and immunogenicity of the live oral attenuated cholera vaccine candidate strain vibrio cholerae cvd 103-hgr (classical, inaba). a single dose of 5 x 10(8) viable cvd 103-hgr organisms, administered in a buffered liquid formulation, was well tolerated as compared with individuals who received an equivalent amount of heat-killed escherichia coli k-12 placebo. eighty-eight percent of s ...	1990	2087879
[the auscultation method of measuring arterial blood pressure (history of its invention and the current aspects of its use].		2007	2084325
pseudocoarctation of the aorta.	in this report 5 patients between the ages of 5-14 years admitted to the department of pediatric cardiology, hacettepe university, and diagnosed as having pseudocoarctation of the aorta are presented. the clinical and angiographic findings of this rare abnormality of childhood are discussed, the importance of differential diagnosis of this entity from coarctation of the aorta is emphasized, and the literature reviewed.	2007	2084283
[pulmonary carcinomatous lymphangitis and renal adenocarcinoma].	the case of a 7 year-old who presented with lymphangitic carcinomatosis revealed by lung biopsy and secondary to renal adenocarcinoma is described. this biopsy was performed because of radiologic and clinical signs associating bilateral interstitial infiltrates, mediastinal adenopathy and gradually increasing respiratory distress. despite treatment, the child died two weeks later.	2006	2082848
pseudo-chediak-higashi anomaly in acute myeloid leukemia (m2) of childhood.	the frequency and clinical significance of the pseudo-chediak-higashi (pch) anomaly were studied in 20 children with acute myeloid leukemia (aml) m2 in the fab nomenclature. pch granules were recognized as giant eosinophilic granules, measuring up to 5 microns, in the cytoplasm of leukemic cells on smears. at the electron microscope level, most pch granules were round to oval and outlined by a limiting membrane, and contained homogeneous, granular, crystalloid, rod-like or myelin-like materials. ...	2006	2082666
effect of alprazolam on pentylenetetrazol convulsions.		1990	2082648
detection and interpolation of outliers in biosignals.	detection of outliers in recorded biosignals and their interpolation is described. the detection of outliers is based on the simple rule of 3 sigma. the subsequent interpolation is performed according to the method proposed by brubacher and wilson for autoregressive processes. the output of the suggested procedure can be treated by standard programs of statistical analysis as long as the influence of outliers is eliminated. the program in fortran iv developed in the institute of hygiene and epid ...	1990	2082646
[relationship between the anti-hemolysin activity and the structure of catechins and theaflavins].	we examined the corresponding isomers of catechins and theaflavins for anti-hemolysin activities against staphylococcus aureus alpha-toxin and vibrio cholerae o1 hemolysin. catechins and theaflavins showed anti-hemolysin activities in a dose-dependent manner. among the catechins tested, (-)catechin gallate, (-)epicatechin gallate and (-)epigallocatechin gallate having galloyl groups in their molecules showed more potent anti-hemolysin activities against both toxins. on the other hand, free catec ...	1990	2082027
[chemical and serological studies of lipopolysaccharides isolated from o1 and non-o1 vibrio cholerae].		1990	2082025
dna damage and cell killing by nitrofurantoin in relation to its carcinogenic potential.	nitrofurantoin inhibited growth and produced loss of viability of vibrio cholerae cells in a dose-dependent manner, the 10% (d10) and 37% (d37) survival doses being 18.0 and 5.5 micrograms/ml x hr. respectively. the drug also caused filamentation of the cells in a very significant manner. ultraviolet absorption data and thermal chromatography through hydroxyapatite column revealed that nitrofurantoin treatment of vibrio cholerae cells produced a maximum amount of 55% of dna reversibly bihelical ...	1990	2081336
ovine white-liver disease (owld). treatment trials.		1990	2080781
[a case of acute leukemia with spinal cord involvement].		2007	2080586
homocysteic acid as an endogenous excitatory amino acid.		1990	2080552
genetic analyses of rhizobium meliloti exopolysaccharides.	we have recently obtained strong genetic evidence that the acidic calcofluor-binding exopolysaccharide (eps i) of rhizobium meliloti rm1021 is required for nodule invasion and possibly for later events in nodule development. thirteen loci on the second megaplasmid have been identified that are required for, or affect, the synthesis of eps i. mutations in certain of these loci completely abolish the production of eps i and result in mutants that form empty fix- nodules. exoh mutants fail to succi ...	1990	2078533
[the genetic and pathogenetic mechanisms of atherosclerosis].		2007	2077272
a vasopressin and oxytocin containing nucleus in the pig hypothalamus that shows neuronal changes during puberty.	a vasopressin and oxytocin containing nucleus is described for the first time in the pig hypothalamus. it is located near the third ventricle, just dorsal to the suprachiasmatic nucleus, and consists of magnocellular neurons, similar to those of the supraoptic nucleus and paraventricular nucleus. morphometric analysis of neuronal number, size, density, and volume was performed at four different ages: 1 day, 7 weeks, 16 weeks, and 30 weeks postnatally. no sex difference in these parameters was ob ...	1990	2077051
[recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation].	every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ...	1990	2075365
[age-sex aspects of distribution of children with scleroderma according to clinical forms].		2007	2075074
exercise performance and limiting factors in patients with chronic lung diseases.	to demonstrate the clinical usefulness of the treadmill exercise test in patients with chronic lung diseases (cld), 272 consecutive tests done from jan. 1986 to dec. 1988 were analyzed. the subjects mainly consisted of patients with chronic obstructive pulmonary disease (copd) and interstitial lung disease (ild). with the present incremental treadmill protocol the maximal stage attained by a patient or distance walked were closely related to the patient's maximal oxygen uptake. comparing symptom ...	2007	2074970
scalp cooling by cold air for the prevention of chemotherapy-induced alopecia.	a new system is described for cooling the scalp with cold air in order to prevent chemotherapy-induced alopecia. compressed air was cooled by means of a vortex tube built into a hair-drier cap. this system reduced the blood flow in the scalp to 35%, the surface temperature to 14.2 degrees c and the intradermal temperature at hair follicle level to 29.2 degrees c. the low temperature could be kept constant for at least one hour of cooling. by means of comparison, with cryogel packs the lowest epi ...	2007	2074916
cholera--new york, 1991.	through june 26, 1991, cholera has been reported from seven countries in the western hemisphere: brazil, chile, colombia, ecuador, mexico, peru, and the united states. in the united states, a total of 14 confirmed cases of epidemic-associated cholera have been reported among persons in florida (one) (1), georgia (one) (2), new jersey (eight) (1), and new york (four). this report summarizes information regarding the four cases reported in new york and describes a new laboratory procedure used to ...	1991	2072887
arytenoidectomy and posterior cordectomy for bilateral abductor paralysis.	twelve patients with bilateral fixed vocal cords seeking decannulation of their tracheostomies were subjected to endoscopic correction of the compromised airway. they all had arytenoidectomy associated with posterior cordectomy whether as a primary or secondary stage procedure. decannulation was achieved in all patients using the technique described.	2007	2072014
[surveys on the contamination of marine fish with non-o1 vibrio cholerae and vibrio mimicus and food poisoning cases by these organisms].	the present paper describes the relationship between the contamination with non-o1 vibrio cholerae and vibrio mimicus of marine fish, with special reference to the seasonal variation and the concentration of contamination, and the actual cases of domestic food poisoning by these organisms. a 10 year survey revealed that non-o1 vibrio cholerae (non-o1 v. cholerae) strains were frequently isolated from fish during the summer season with some variations from one year to another, and isolates from f ...	1991	2066602
effects of vibrio cholerae recombinant strains on rabbit ileum in vivo. enterotoxin production and myoelectric activity.	previous studies have identified the effects of vibrio cholerae and its enterotoxin, choleragen (ct a+b+), on the myoelectric activity of rabbit ileal loops in vivo. the response was defined as the migrating action potential complex, the single ring contraction that propels luminal contents aborad. in this study the same rabbit model is used to assess whether migrating action potential complex activity or fluid output is induced by recombinant strains of v. cholerae that produce no subunit of ch ...	1991	2065905
epidermal cytokines and their roles in cutaneous wound healing.	cytokines are small proteins or glycoproteins which are synthesized and secreted by a variety of cell types. through binding to specific receptors on target cells, these hormone-like products regulate many normal cell activities, including growth and differentiation, migration and immune functions. within the epidermis, keratinocytes are the major source of cytokines along with melanocytes and langerhans cells. in response to a variety of injurious stimuli, including ultraviolet irradiation and ...	1991	2064935
the production of transgenic mice by embryo microinjection.	the production of transgenic mice is a technology of great utility in the dissection of complex biological processes. this article is intended as a detailed primer for people interested in learning to produce transgenic mice, and discusses equipment, methods, and future directions for this technique.	2007	2064823
restrained eating, obesity, and cumulative food intake curves during four-course meals.	a four-course meal was presented to six obese and 18 normal weight women in which the second course was eaten ad libitum and the other three courses were fixed in amount. eating behaviour was observed directly and intake was monitored via an electronic scale built into the table under the plate. intake deceleration was observed in the normal weight women who scored low on the herman-polivy (1980) restraint questionnaire and on the cognitive restraint factor (f1) of stunkard and messick's (1985) ...	2006	2064393
rubinstein-taybi syndrome with de novo reciprocal translocation t(2;16)(p13.3;p13.3).	we describe a girl with typical rubinstein-taybi syndrome with apparently balanced reciprocal translocation between chromosome 2 and 16. the patient has a condition characterized by mental retardation, typical facial manifestations, broad thumbs and first toes. cytogenetic studies of the patient showed a reciprocal translocation without visible deletion, karyotype: 46,xx, t(2;16)(p13.3;p13.3). her parents had normal chromosomes. these results suggest that the locus of the gene for the rubinstein ...	1991	2063911
cholera and cholera-like diarrhoeal diseases: why the differences in severity?	although vibrio cholera and several other enterotoxigenic bacteria such as the enterotoxigenic escherichia coli produce secretory diarrhoea by exactly the same mechanism, cholera is much more severe. it is proposed that this difference in severity may be because while the alkaline diarrhoeal fluid produced in these conditions is optimal for the growth of vibrio cholera, e. coli and other enterotoxigenic bacteria are inhibited.	1991	2062265
a method for studies of an el tor-associated antigen of vibrio cholerae o1.	a method for studying the biotype el tor associated mannose-sensitive haemagglutinin (msha) of v. cholerae o1 has been developed. by using crude msha adsorbed to chicken erythrocytes as solid phase antigen in an enzyme-linked immunosorbent assay (elisa), antisera against v. cholerae of the el tor biotype reacted in high titre with the msha-coated cells, whereas antisera against vibrios of the classical biotype did not bind significantly, i.e. in higher titre than pre-immune sera. the binding of ...	1991	2060759
evaluation of the antimicrobial activity of methylglyoxal bis(guanylhydrazone) analogues, the inhibitors for polyamine biosynthetic pathway.	metabolic and antiproliferative effects of methylglyoxal bis(butylamidinohydrazone) (mgbb) and methylglyoxal bis(cyclopentylamidinohydrazone) (mgbcp), inhibitors for polyamine biosynthetic pathway, on escherichia coli, shigella sonnei, aeromonas sobria, aeromonas hydrophila and vibrio cholerae were investigated. mgbb at the concentration of 100 mumol/l depleted intracellular putrescine and spermidine concentrations of e. coli to 25 and 20% of the controls, respectively, while mgbcp depressed the ...	1991	2055790
risk of transmission of cholera by foods.		1991	2054288
regulatory cascade controls virulence in vibrio cholerae.	expression of more than 17 virulence genes in vibrio cholerae is under the coordinate control of the toxr protein. toxr is a transmembrane protein that binds to and activates the promoter of the operon encoding cholera toxin. as yet, the ability of toxr to activate directly other genes in this regulon has not been demonstrated. we have cloned a gene called toxt from v. cholerae 569b; the toxt gene product, like toxr, can activate the ctx promoter in escherichia coli. in addition, expression of o ...	1991	2052618
vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions.	attenuated vibrio cholerae vaccine strains specifically mutated in genes encoding cholera toxin (ct) are still capable of causing mild to moderate diarrhea. culture supernatants of v. cholerae strains, both ct-positive and ct-negative, were examined in ussing chambers, and a toxin was found that increases the permeability of the small intestinal mucosa by affecting the structure of the intercellular tight junction, or zonula occludens. the activity of this toxin is reversible, heat-labile, sensi ...	1991	2052603
duodenal rapeseed oil infusion in early and midlactation cows. 1. intestinal apparent digestibility of fatty acids and lipids.	rapeseed oil was infused continuously into the duodenum of lactating dairy cows. five separate trials were conducted with differences in amount of oil infused (1.0 to 1.5 kg/d), in lactation stage (early to late), and in basal diet (corn or grass silages). the effects of lecithin addition (9 g/d) also were studied. oil infusion did not affect the apparent digestibility of defatted organic matter. fatty acid, ether extract, and total lipid digestibilities in control cows were 62 to 82%, 72 to 76% ...	1991	2045558
cloning and nucleotide sequence of the vibrio cholerae hemagglutinin/protease (ha/protease) gene and construction of an ha/protease-negative strain.	the structural gene hap for the extracellular hemagglutinin/protease (ha/protease) of vibrio cholerae was cloned and sequenced. the cloned dna fragment contained a 1,827-bp open reading frame potentially encoding a 609-amino-acid polypeptide. the deduced protein contains a putative signal sequence followed by a large propeptide. the extracellular ha/protease consists of 414 amino acids with a computed molecular weight of 46,700. in the absence of protease inhibitors, this is processed to the 32- ...	1991	2045361
no time for an aids backlash.		2007	2045284
[health indicators in urban areas. variations in function of social coherence of the geographic areas used].	the detection of health inequalities in the urban environment and their magnitude depends to a great extent on the internal social coherence of the geographical division used. using an existing social map of alicante which divides the city into basic units for social intervention (busi), we have compared the distribution of an indicator with wellknown relationship with the socio-economic level such as low birth weight, using both the busi and the municipal districts as analysis units. the data o ...	2007	2045222
dna adducts in bronchial biopsies.	to investigate the feasibility of measuring dna-carcinogen adducts in the lungs of non-surgical patients, endobronchial biopsies were obtained from 78 patients undergoing routine diagnostic bronchoscopy. lung cancer was present in 37 (47%) of the patients. dna was isolated from the tissues and analyzed by hplc- or nuclease-pi-enriched 32p-postlabelling, using procedures selective for aromatic adducts. chromatograms from all 28 current smokers showed a distinctive diagonal adduct zone which was p ...	2007	2045196
effects of an endogenous ouabainlike compound on heart and aorta.	an endogenous ouabainlike compound (olc) has been purified from human plasma, and mass spectrometry has shown it to be indistinguishable from plant-derived ouabain. this human olc was tested for its effects on evoked tension in guinea pig left atria and aortic rings. the tissues were incubated at 37 degrees c in bicarbonate-buffered physiological salt solution gassed with 95% o2-5% co2. in atria stimulated electrically at 1 hz, 85 and 170 nm human olc increased peak active force to 177 +/- 15% a ...	1991	2045174
glucagon and clonidine testing in the diagnosis of pheochromocytoma.	we assessed the sensitivity and specificity of glucagon stimulation and clonidine suppression tests in the diagnosis of pheochromocytoma in 113 hypertensive patients, 39 with and 74 without the tumor. in the glucagon stimulation test, blood was sampled 2 minutes after intravenous injection of 0.28 mumol (1 mg) glucagon, and in the clonidine suppression test, blood was sampled 3 hours after administration of oral clonidine, 1.30 mumol (0.3 mg)/70 kg body wt. baseline levels of catechols in antecu ...	2007	2045133
cardiac output during exercise in paraplegic subjects.	the purpose of this study was to measure the cardiac output using the co2 rebreathing method during submaximal and maximal arm cranking exercise in six male paraplegic subjects with a high level of spinal cord injury (hp). they were compared with eight able bodied subjects (ab) who were not trained in arm exercise. maximal o2 consumption (vo2max) was lower in hp (1.11.min, sd 0.1; 17.5 ml.min-1.kg-1, sd 4) than in ab (2.5 l.min-1, sd 0.6; 36.7 ml.min-1.kg, sd 10.7). maximal cardiac output was si ...	1991	2044535
altitude diuresis: endocrine and renal responses to acute hypoxia of acclimatized and non-acclimatized subjects.	as a result of our recently published studies we have thought that altitude diuresis resulting from hypoxic stimulation of the arterial chemoreceptors reduces the cardiac volume overload. to test this hypothesis, cardiovascular, endocrine and renal responses to stepwise acute exposure to simulated altitude (6,000 m) were compared in ten acclimatized recumbent mountaineers a mean of 24 days, sd 11, after descending from himalayan altitudes of at least 4,000 m, with those found in ten non-acclimat ...	2007	2044531
static and dynamic assessment of the biodex dynamometer.	the validity and accuracy of the biodex dynamometer was investigated under static and dynamic conditions. static torque and angular position output correlated well with externally derived data (r = 0.998 and r greater than 0.999, respectively). three subjects performed maximal voluntary knee extensions and flexions at angular velocities from 60 to 450 degrees.s-1. using linear accelerometry, high speed filming and biodex software, data were collected for lever arm angular velocity and linear acc ...	2007	2044524
biphasic changes in leukocytes induced by strenuous exercise.	seven healthy male volunteers participated in short- (str, 1.7 km), middle- (mtr, 4.8 km) and long- (ltr, 10.5 km) term runs at a speed close to their maximum. a prompt mobilization of white cells, and lymphocytes in particular, appeared following the exercise. the initial increase in the number of lymphocytes was succeeded by a significant decrease [(p less than 0.03) lymphopenial], which on average was 32%-39% of the pre-exercise values in all groups. a close correlation was found between the ...	2007	2044521
sir frederick hobday memorial lecture. all wind and water: some progress in the study of equine gut motility.		1991	2044514
mind over muscle.		1991	2044511
the stanford experience with high-dose etoposide cytoreductive regimens and autologous bone marrow transplantation in hodgkin's disease and non-hodgkin's lymphoma: preliminary data.	seventy-seven hodgkin's disease and non-hodgkin's lymphoma (nhl) patients received high-dose etoposide in combination with cyclophosphamide and either fractionated total body irradiation (tbi) (n = 28) or carmustine (n = 49) prior to autologous bone marrow transplantation. marrow from nhl patients was purged in vitro with a panel of monoclonal b- and t-cell antibodies and complement. six toxic deaths (8%) occurred, all in patients who received carmustine. with a median follow-up of 1 year, 57 pa ...	2007	2043498
detection of chlamydia trachomatis in first-void urine collected from men and women attending a venereal clinic.	cervical, urethral and first-void urine (fvu) specimens from 196 men and 245 women attending a venereal outpatient clinic were studied by culture and a commercial enzyme immunoassay (eia) (chlamydiazyme). confirmatory chlamydial testing by a direct fluorescence assay (dfa) (microtrak) was performed on the sediments of the positive eia samples from culture-negative patients. chlamydia trachomatis was isolated from 11% of the men and 12% of the women. of the women, 67% were positive in both sampli ...	1991	2043357
abrogation of dominant glucose intolerance in sjl mice by a growth hormone transgene.	glucose tolerance was studied in transgenic mice (sjl x c57bl/6) expressing human gh under the control of a housekeeping promoter. parental sjl mice were found to harbour a dominant allele, termed here glid, determining glucose intolerance in pure-bred animals and in f1 hybrids with glucose-tolerant c57bl/6 mice. blood glucose levels in transgenic sjl x c57bl/6 hybrid mice were well controlled following glucose challenge, whereas non-transgenic hybrids failed to control their glucose adequately. ...	1991	2043241
[postpolio muscular atrophy].		2007	2042161
ovarian cancer.		2007	2042058
[operative arthroscopy of the ankle. 107 cases].	ankle arthroscopy was performed in 127 patients. it was possible to evaluate 107 of these patients. follow-up covered a mean period of 39 months (12 to 94 months). a figure of 80% good results was found in disorders such as osteochondral fractures of the dome of the talus, anterior osteophytosis of the tibia or after synovectomy with arthroscopic direct vision in synovial diseases such as benign tumours or chronic inflammatory rheumatism. the role of arthroscopy in ankle instability and in osteo ...	2007	2042013
production of monoclonal antibodies against a hemagglutinin/protease of vibrio cholerae non-01.	two hybridoma cell lines producing monoclonal antibodies (mabs) against a hemagglutinin/protease (ha/p) from vibrio cholerae non-01 were produced and characterized. the two mabs contained the kappa light chain and were igg1 type. they similarly neutralized ha/p protease activity derived from both v. cholerae non-01 and v. cholerae 01, whereas they were unable to neutralize the hemagglutinating activity of ha/p, suggesting that the epitopes for protease and hemagglutination activities are differe ...	1991	2040430
experimental liver cysts induced by 2-acetylaminofluorene.	liver cyst formation was studied serially in an experimental model in which rats were fed a diet containing 0.02% 2-acetylaminofluorene, a carcinogen, for 6 weeks, followed by a normal diet for 42 weeks. cysts appeared in the portal tracts at the 12th week, by which time the intrahepatic bile ducts had also proliferated and dilated. some of the dilated bile ducts were cavernous or multicystic and appeared to represent a transitional form between the bile duct dilation and cystic formation. after ...	1991	2040399
human immunodeficiency virus infection in pregnancy.	many aspects of hiv infection in pregnancy remain unclear. subsets at increased risk for perinatal transmission, adverse pregnancy outcome, and development of symptomatic hiv infection need to be identified. for instance, relative risks may be quite different in asymptomatic hiv infected patients with t4 lymphocyte counts greater than 200 cells per cubic millimeter compared to those with either symptoms of hiv infection or t4 cell counts less than 200 cells per cubic millimeter. at present, anti ...	1991	2040117
strain specific sensitivity to diethylnitrosamine-induced carcinogenesis is maintained in hepatocytes of c3h/hen in equilibrium with c57bl/6n chimeric mice.	the c3h/hen (c3h) and c57bl/6n (c57) mouse strains are known, respectively, for their high and low susceptibility to both spontaneous and chemically induced hepatocarcinogenesis. the present study was aimed at elucidating whether this difference is dependent on intrinsic features of the target hepatocytes or in the in vivo milieu and associated growth promoting factors to which the cells are exposed. c3h in equilibrium with c57 chimeric mice were produced and given injections of diethylnitrosami ...	1991	2040001
a library for the fifteenth through the twenty-first centuries.	the university of california, san francisco (ucsf), began developing a program for a new library in 1977, started the design in 1985, began construction in 1988, and opened the library in september 1990. the primary objectives were to design and build a facility that would house print collections under optimal conditions, allow for ten years' growth, be flexible enough to permit future reconfiguration, support present and future technologies, and provide beautiful spaces in which to study. the p ...	2006	2039900
field trial of oral cholera vaccines in bangladesh: serum vibriocidal and antitoxic antibodies as markers of the risk of cholera.	the relationship of serum vibriocidal (vc) and igg anti-cholera toxin (ct) antibodies to the risk of cholera was evaluated during the first year of follow-up of recipients of three oral doses of b subunit (bs)-whole-cell vaccine, whole-cell vaccine, or escherichia coli k12 strain placebo in bangladesh. acute sera from 121 cholera patients were compared with sera from 2592 contemporaneous community controls. each doubling of vc titer was associated, on average, with a 22%-47% reduction of cholera ...	1991	2037789
purification and characterization of a new heat-stable enterotoxin produced by vibrio cholerae non-o1 serogroup hakata.	the possible production of a heat-stable enterotoxin (vc-h-st) by vibrio cholerae non-o1 serogroup hakata was investigated, and the purified vc-h-st was characterized. it has a unique amino acid sequence, lidcceiccnpacfgcln. this sequence is quite similar to that of the heat-stable enterotoxin (nag-st) produced by v. cholerae non-o1 except for one amino acid (leucine) residue excess at the n terminus. other characteristics, including biological activity, are compatible with those of nag-st.	1991	2037379
enterotoxin-associated dna sequence homology between salmonella species and escherichia coli.	multiple hindiii-restriction fragments of salmonella typhimurium and salmonella typhi chromosomal dna exhibited homology with the heat-labile enterotoxin (lt1) gene of escherichia coli as determined by southern blot analysis. a 9.4 kb hindiii restriction fragment identified in s. typhimurium and s. typhi chromosomal dna reacted with both elta and eltb gene probes. however, the homology of the 9.4 kb dna fragment from these salmonella species was greater with eltb than elta. in addition, a synthe ...	1991	2037224
tumor necrosis factor alpha selectively sensitizes human immunodeficiency virus-infected cells to heat and radiation.	we report here that infection of the human t-cell line hut-78 with human immunodeficiency virus (hiv) increases its sensitivity to heat and radiation toxicity. a possible explanation for this result may be the reduced expression of manganous superoxide dismutase (mnsod) in hiv-infected cells compared to uninfected cells. tumor necrosis factor alpha (tnf-alpha) further sensitizes hiv-infected cells but not uninfected cells to heat and radiation. this is consistent with the ability of tnf-alpha to ...	1991	2034677
characterization of genetic variation and 3'-azido-3'-deoxythymidine- resistance mutations of human immunodeficiency virus by the rnase a mismatch cleavage method.	the rnase a mismatch cleavage method has been applied to the characterization of natural genetic variation of human immunodeficiency virus (hiv) from different geographical areas. the approach provides a rapid and simple assay for the analysis of differences in closely related viral isolates and allows the establishment of phylogenetic relationships between epidemiologically distinct viruses. our results show a broad clustering of circulating viruses according to their geographical distribution. ...	1991	2034672
cholera mystery.		2007	2034267
synthesis in vibrio cholerae and secretion of hepatitis b virus antigens fused to escherichia coli heat-labile enterotoxin subunit b.	a simple and effective electroporation method for the transformation of vibrio cholerae with nonmobilizable plasmids is described. expression plasmids directing the synthesis of fusion proteins with the subunit b of escherichia coli heat-labile enterotoxin b (lt-b) were transformed into nontoxinogenic v. cholerae vaccine strains. a protein consisting of two overlapping immunodominant antibody-binding sites of the hepatitis b virus (hbv) middle surface antigen fused to the c terminus of full-leng ...	1991	2022336
cholera. small risk of cholera transmission by food imports.		1991	2021526
validation of biologic markers for use in research on chronic fatigue syndrome.	unresolved aspects of chronic fatigue syndrome can be addressed by research involving biologic markers. these may be any molecular, biochemical, physiological, or other biologic parameter obtainable from biologic specimens. the use of biologic markers in research requires their validation as dependent or independent variables. additionally, other characteristics of markers such as reliability of assays, background level, confounding factors, interpretations, and legal and ethical implications sh ...	2007	2020808
the protective activity of tea against infection by vibrio cholerae o1.	extracts of black tea exhibited bactericidal activity against vibrio cholerae o1. the tea extract inhibited the haemolysin activity of v. cholerae o1, el tor and the morphological changes of chinese hamster ovary cells induced by cholera toxin. tea extract also reduced fluid accumulation induced by cholera toxin in sealed adult mice and by v. cholerae o1 in ligated intestinal loops of rabbits. these findings suggest that tea has protective activity against v. cholerae o1.	1991	2019547
preferential reactivity of autoantibodies in murine lupus nzb mice to neuraminidase-treated monosialogangliosides on b cells of mouse spleen.	when analyzed by flow cytometry, reactivity of igm autoantibodies in sera from nzb mice to spleen b cells, but not to t cells, from balb/c mice was remarkably increased after treatment of the cells with vibrio cholerae neuraminidase. by tlc immunostaining with the antibodies, neither neutral nor acidic glycosphingolipids from both balb/c and nzb mouse spleens were found to be reactive, but after neuraminidase treatment of the tlc plate, prior to the immunostaining, three components became reacti ...	1991	2018978
production and characterization of monoclonal antibodies to vibrio cholerae soluble haemagglutinin.	monoclonal antibodies (mabs) against a protease of vibrio cholerae, the soluble haemagglutinin (sha), have been prepared and characterized with regard to their ability to inhibit different biological properties of sha and to protect against experimental v. cholerae infection. four fusion experiments yielded two specific immunoglobulin g1 mabs that reacted with sha produced by different v. cholerae o1 and non-o1 strains but that differed in their antigen-binding capacity. both mabs were capable o ...	1991	2018638
plasma amine oxidase activities in norrie disease patients with an x-chromosomal deletion affecting monoamine oxidase.	two individuals with an x-chromosomal deletion were recently found to lack the genes encoding monoamine oxidase type a (mao-a) and mao-b. this abnormality was associated with almost total (90%) reductions in the oxidatively deaminated urinary metabolites of the mao-a substrate, norepinephrine, and with marked (100-fold) increases in an mao-b substrate, phenylethylamine, confirming systemic functional consequences of the genetic enzyme deficiency. however, urinary concentrations of the deaminated ...	2007	2018626
identification of the genomic region determining serotype specificity of vibrio cholerae 01.	a 2.1-kb genomic region responsible for ogawa serotype specificity of vibrio cholerae 01 was identified by cosmid cloning and recombinant plasmid experiments. the plasmid carrying this region derived from ogawa type vibrio cholerae nih 41 coded for a specific protein of 27 kd, and was found to convert serotype specificity from inaba to ogawa when co-introduced into the escherichia coli cells harboring a cloned 20-kilobase genomic dna fragment of inaba type vibrio cholerae 35a3.	1991	2018510
synthetic peptides as the basis for vaccine design.		1991	2017193
[the visit of the soviet military medical delegation to china].		2006	2014671
purification and characterization of a secreted protease from the pathogenic marine bacterium vibrio anguillarum.	vibrio anguillarum is a pathogenic marine bacterium which causes the disease vibriosis in salmonid fish, which is characterized by a fatal hemorrhagic septicemia accompanied by massive tissue destruction. in this paper, the purification of the major caseinolytic extracellular protease from v. anguillarum is presented. the purification steps include ammonium sulfate precipitation, deae-sepharose chromatography, sephacryl s-200 chromatography, and deae high-pressure liquid chromatography. the puri ...	1991	2012804
magnetic resonance imaging: a new approach for evaluating coronary artery disease?		1973	2008844
immunogenicity of two formulations of oral cholera vaccines in thai volunteers.	a formulation of oral vaccine consisting of vibrio cholerae lipopolysaccharides (lps), cell-bound haemagglutinin (cha) and procholeragenoid (p), namely vaccine a, was compared with another formulation, vaccine b, prepared from killed whole vibrios plus procholeragenoid on their immunogenicity and reactogenicity in thai male volunteers. volunteers were randomly allocated into three groups. the first two groups received orally three doses of vaccines a and b, respectively at 14-day intervals. volu ...	1991	2008802
aircrew decision-making behavior in hazardous weather avoidance.	in-flight encounters with hazardous weather represent one of the most significant safety issues in civil aviation operations. aircrew judgment is often cited as the probable cause of incidents and accidents involving weather, although lack of information is also a factor. the present study examines how information, presented at different times and in different forms, affects the awareness and decision-making behavior of aircrews in a flight simulation study of a recent microburst/windshear incid ...	2007	2001213
[the effect of the application technic on the formal success of large-field radiotherapies].	198 patients suffering from hodgkin's disease or non-hodgkin's lymphomas were irradiated with large-field techniques from 1979 to 1988. 129 of these were treated with common block techniques whereas the field-integrated dose modification (fidm) was applied to 69 patients. retrospective analysis shows that fidm decreases acute toxicity. this was also evident in those patients who were irradiated by accelerated hyperfractionation. although single doses were higher in patients treated with fidm tre ...	2007	2000545
direct evidence that ganglioside is an integral component of the thyrotropin receptor.	gangliosides were extracted from purified human and porcine thyrotropin (tsh) receptors (tsh-r) and were detected by probing with an 125i-labeled sialic acid-specific lectin, limax flavus agglutinin. gangliosides copurified with human and porcine tsh-r migrated between monosialoganglioside gm1 and disialoganglioside gd1a. ceramide glycanase digestion of the purified human tsh-r-associated glycolipid confirmed its ganglioside nature. it was resistant to vibrio cholerae sialidase, which digests al ...	1991	2000404
toxr regulates the production of lipoproteins and the expression of serum resistance in vibrio cholerae.	the genes encoding three lipoproteins of vibrio cholerae were identified by a combination of dna sequence analysis and [3h]palmitate labeling of hybrid proteins encoded by tnphoa gene fusions. the expression of these three lipoproteins, taga, acfd, and tcpc, was controlled by toxr, the cholera toxin transcriptional activator. the involvement of other bacterial lipoproteins in conferring resistance to the bactericidal effects of complement prompted us to examine this possibility in v. cholerae. r ...	1991	2000374
freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles.	freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ...	1991	1999383
[prolongation of hospital stay by nosocomial pneumonia and wound infection].	from june 1988 to september 1989, a prospective survey comprising a total of 1183 patients in a university hospital was carried out to ascertain the additional length of stay in intensive care units because of nosocomial pneumonia associated with artificial ventilation (418 patients, 296 men, 122 women, mean age 48.8 +/- 21 years, ventilated for more than 24 h) or by postoperative wound infections (765 patients, 501 men, 264 women, mean age 60 +/- 11 years, after operations on the large bowel, h ...	2006	1997295