Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| mu-opioid receptors mediate the inhibitory effect of opioids on dopamine-sensitive adenylate cyclase in primary cultures of rat neostriatal neurons. | the receptors mediating the inhibition of d1 dopamine receptor-stimulated adenylate cyclase by opioids were examined in primary cultures of rat neostriatal neurons. adenylate cyclase activity was dose-dependently increased by the selective d1 dopamine receptor agonist skf 38393 (ec50 = 0.05 microm). this stimulation was fully antagonized by the selective d1 dopamine receptor antagonist sch 23390 (1 microm). skf 38393 (1 microm)-stimulated adenylate cyclase activity was strongly reduced (by almos ... | 1990 | 2168934 |
| establishment of ddd/1-mtv-2/mtv-2, nu/nu and ddd/1-mtv-2/mtv-2, nu/+ mice: preliminary characterization in relation to mtv antigen expression and mammary tumorigenesis. | to investigate the effects of the t-cell deprivation on viral mammary tumorigenesis, two double congenic mouse strains of the ddd genetic background, ddd/1-mtv-2/mtv-2, nu/nu and ddd/1-mtv-2/mtv-2, nu/+, were produced by the cross between ddd/1-mtv-2/mtv-2 (ddd-mtv-2) and ddd/1-nu/nu mice, followed by repeated intercross breedings. expression of the mouse mammary tumor virus (mtv)-gp52 antigen was demonstrated in the mammary glands of mice from 14 days on, in both -nu/nu and -nu/+ females. mamma ... | 1990 | 2168807 |
| role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry. | the dynamics of the enthalpy and volume changes found in the photodissociation of co from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. an intermediate is observed whose lifetime at 20 degrees c is 700 ns. the enthalpy of the intermediate increases by approximately 7 kcal/mol upon replacing arginine-45 with either asparagine or glycine. these observations support recent proposals th ... | 2007 | 2168740 |
| dna methylation pattern during the encystment of physarum flavicomum. | in physarum flavicomum berk., haploid myxamoebae convert to dormant microcysts under conditions of nutrient imbalance. exogenous adenine increases the intracellular content of s-adenosylmethionine (sam) and inhibits this process. however, treatments that reduce the intracellular sam levels relieve the inhibition of encystment induced by adenine. sam plays a major metabolic role in cellular transmethylation reactions. in this study, we compared the dna methylation patterns of growing cells, encys ... | 2007 | 2168719 |
| antiinflammatory properties of the new antirheumatic agent 4-acetylaminophenylacetic acid. | the antiinflammatory activity of 4-acetylaminophenylacetic acid (ms-932) was investigated. ms-932 did not suppress the acute inflammation of carrageenin-induced paw edema in rats or of primary swelling in adjuvant arthritic rats. however, prophylactic treatment with ms-932 inhibited secondary inflammation in adjuvant arthritic rats. ms-932 also restored to normal the weight of the spleen and the serum albumin/globulin ratio of adjuvant arthritic rats. in addition to its prophylactic effect, ms-9 ... | 1990 | 2168705 |
| congenital multiple plaquelike glomus tumors. | we encountered two patients with congenital multiple plaquelike glomus tumors. these lesions were present at birth and enlarged with body growth, attaining a diameter of up to 13 cm. the diagnosis was confirmed by histologic and electron-microscopic examination, which revealed the typical changes of glomus tumors. immunocytochemistry findings demonstrated the tumor cells to be vimentin- and alpha-smooth muscle-actin-positive and desmin-negative. on ultrastructural examination, typical dense bodi ... | 2007 | 2168692 |
| phosphonylmethyl ethers of acyclic nucleoside analogues: inhibitors of hsv-1 induced ribonucleotide reductase. | diphosphates of n-(s)-(3-hydroxy-2-phosphonylmethoxypropyl) and n-(2-phosphonylmethoxyethyl) derivatives of purine and pyrimidine heterocyclic bases inhibit hsv-1 encoded ribonucleotide reductase. of the compounds studied, the most efficient inhibitors of cdp reduction (at 5.1 mumols.l-1) by the hsv-1-encoded enzyme are hpmpapp (ic50 = 0.9 mumols.l-1) and pmeapp (ic50 = 8 mumol.l-1). pmeapp does not inhibit the enzyme isolated from the mutant hsv-1 kos strain pmear which is resistant to pmea at ... | 1990 | 2168691 |
| proton-nuclear magnetic resonance relaxation times in brain edema. | proton relaxation times of protein solutions, bovine brain, and edematous feline brain tissue were studied as a function of water concentration, protein concentration, and temperature. in accordance with the fast proton exchange model for relaxation, a linear relation could be established between r1 and the inverse of the weight fraction of tissue water. this relation also applied to r2 of gray matter and of protein solutions. no straightforward relation with water content was found for r2 of wh ... | 1990 | 2168666 |
| effect of superoxide dismutase in cats with cold-induced edema. | we evaluated the potential role of sod, an oxygen free radical scavenger, as a probe to cover the trigger period of injury and the prolonged period of development of edema in vasogenic brain edema. vasogenic brain edema was produced in 34 cats by a standardized cortical freezing lesion. brain edema and the disruption in the bbb were assessed by sg measurement and spread of evans blue by planimetry. detection of superoxide radicals was studied by topical application of nbt within the cranial wind ... | 1990 | 2168663 |
| mechanisms underlying the stimulation of pth release by gppnhp in permeabilized bovine parathyroid cells. | we examined changes in camp and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (g) protein(s) regulating adenylate cyclase and phospholipase c in mediating the stimulatory effects of gppnhp on pth release from permeabilized bovine parathyroid cells. to examine the role of gs, the g protein stimulating adenylate cyclase, and camp on pth release, permeabilized cells were incubated with either gppnhp or isoproterenol, and the effects of these agents on ... | 1990 | 2168660 |
| [brain stem auditory evoked potentials in the early period after a brain concussion]. | acoustic truncal evoked potentials (atep) were studied in 24 patients in the first 48 hours after concussion of the brain. the absolute peak latency of components 1, 3, and 5, the interpeak intervals 1-3, 3-5, and 1-5, and the interaural difference were determined. the results were compared with those of examination of a control group of 24 healthy individuals. individual and group appraisal failed to reveal any significant differences from normal values in none of the studied parameters in pati ... | 2007 | 2168645 |
| [the ultraviolet irradiation of autologous blood and endolymphatic antibiotic therapy in treating pneumonia in patients with craniocerebral trauma]. | on the basis of analysis of 50 cases of craniocerebral injury complicated by pneumonia, the authors prove the efficacy of including ultraviolet irradiation of autologous blood and endolymphatic antibiotic therapy in the complex of therapeutic measures. the mortality and the period of in-hospital treatment of this group of patients reduced. | 2007 | 2168643 |
| [autonomic polyneuropathies in diabetes mellitus (the problems of the clinical picture and treatment)]. | patients with diabetic somatic and vegetative polyneuropathies were subjected to clinical and laboratory studies before and after treatment with the new soviet drug isodibut. the drug has turned out to be highly efficacious in the treatment of vegetative peripheral insufficiency. | 2007 | 2168621 |
| increase of the anion and proton permeability of saccharomyces carlsbergensis plasmalemma by n-alcohols as a possible cause of its de-energization. | the effect of n-alcohols on atp-dependent generation of delta ph and em across the plasma membrane vesicles of the yeast saccharomyces carlsbergensis was investigated. the alcohols were shown to collapse delta ph and em in the order c2 less than c3 less than c4 less than c5 less than or equal to c6 greater than or equal to c7 greater than c8 greater than c11, the dissipation of em being more pronounced. inhibition of the plasmalemma h(+)-atpase was insignificant; at low alcohol concentrations it ... | 1990 | 2168610 |
| protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves. | formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ... | 1990 | 2168606 |
| urinary bladder blood flow. i. comparison of clearance of locally injected 99mtechnetium pertechnate and radioactive microsphere technique in dogs. | the blood flow of the dog urinary bladder measured by radioactive microsphere technique was compared to the clearance of locally injected 99mtechnetium pertechnate (99mtc) in the bladder wall. in semilogarithmic plots the 99mtc washout curves showed a multiexponential course. from the initial slopes (median 5.7 min) the bladder blood flow was calculated to be only 30-62% of the results obtained from the radioactive microsphere technique (blood flow in the muscular layer 21.7-44.8 ml/100 g/min). ... | 1990 | 2168600 |
| chronic myeloid leukemia arising in a progenitor common to t cells and myeloid cells. | until recently, t cells were believed not to be involved in chronic myeloid leukemia. we describe an example of cml in t lymphoblastic crisis with massive generalized lymphadenopathy in which the blasts were cd2(+), cd5(+), and cd7(+), variably cd1(+) and cd3(+), and both responded to and could be induced to produce the t cell growth factor, interleukin-2. additionally, the blasts were shown to contain the cml-related tyrosine kinase p210bcr-abl rather than the smaller kinase associated with ph1 ... | 2007 | 2168506 |
| accuracy and precision of computer-simulated tissue temperatures in individual human intracranial tumours treated with interstitial hyperthermia. | accurate knowledge of tissue temperature is necessary for effective delivery of clinical hyperthermia in the treatment of malignant tumours. this report compares computer-predicted versus measured intratumoral temperatures in 11 human subjects with intracranial tumours, treated with a conceptually simple 'conductive' interstitial hyperthermia system. interstitial hyperthermia was achieved by the use of parallel arrays of implanted, electrically heated catheters. the tissue was warmed by thermal ... | 1990 | 2168460 |
| enhancement by calcium of the detection of cytomegalovirus in cells treated with dexamethasone and dimethyl sulfoxide. | mrc-5 cultures treated with dexamethasone, dimethyl sulfoxide, and calcium were compared with untreated cultures and cultures treated with dexamethasone and dimethyl sulfoxide for the detection of human cytomegalovirus by the shell vial method. the addition of calcium resulted in significantly increased growth of human cytomegalovirus ad169. protein studies and cell counts showed renewed cell proliferation in the treated cultures. when low-titer clinical specimens were retested, 27.5% of all pos ... | 1990 | 2168437 |
| diminished adrenocorticotropin response to insulin-induced hypoglycemia in nondepressed, actively drinking male alcoholics. | although changes in hypothalamic-pituitary-adrenal axis function have frequently been reported in alcoholics, the majority of studies have used recently detoxified subjects in whom abstinence phenomena and clinical depression may contribute to observed stress axis alterations. to isolate the primary effects of alcohol dependence on the stress axis, the acth and cortisol responses to insulin-induced hypoglycemia were measured in seven actively drinking male alcoholics recruited from the general p ... | 2007 | 2168434 |
| somatostatin receptor incidence and distribution in breast cancer using receptor autoradiography: relationship to egf receptors. | somatostatin receptors (ss-r) were measured with in vitro receptor autoradiography using the ss analog 125i-[tyr3]-sms 201-995 as radioligand in 342 breast-tumor samples. in a group of 158 "small" tumor samples (mean section surface: 14 mm2 +/- 0.4; mean +/- sem), 34 tumors (21%) were ss-r positive. in a group of 72 "large" tumor samples (mean size: 180 mm2 +/- 8; mean +/- sem), 33 tumors (46%) were ss-r positive. in this second group, more than half of the tumors had a non-homogeneous distribut ... | 2007 | 2168344 |
| cytomegalovirus nucleic acids in allografted hearts. | 1990 | 2168339 | |
| the antiphospholipid antibody dilemma. | 2007 | 2168305 | |
| [methods of determining clinically sufficient decurarization and its evaluation]. | mechanomyoscopy as an objective method for determining the degree of decurarisation is particularly useful in the case of uncooperative patients and those who are incapable of adequate neck flexion mostly for spinal column diseases. the authors use for that purpose a czechoslovak-made neuromuscular stimulator (lsn 110), with constant 50 hz frequency of stimulation, 0.3 ms impulse duration, max. 50 ma and, depending on skin impedance, 5-140 v. the stimulator switch is manually controlled. the abs ... | 2007 | 2168290 |
| a progesterone-modulated, low-molecular-weight protein from the uterus of the sheep is associated with crystalline inclusion bodies in uterine epithelium and embryonic trophectoderm. | a protein of mr 14,000 (14k protein) has been identified in the medium after culturing sheep conceptuses in vitro. however, it was also a component of uterine flushes of nonpregnant ewes during the luteal phase of the estrous cycle. the 14k protein, which consists of several isoforms, was purified from conceptus culture medium by a simple three-step procedure, involving ammonium sulfate precipitation, high-performance anion-exchange chromatography, and gel filtration on sephacryl s-200. western ... | 1990 | 2168219 |
| vectorial electron and proton transfer steps in the cytochrome bc1 complex. | 1990 | 2168207 | |
| local control of operable breast cancer after radiotherapy alone. | 221 patients with operable breast carcinoma stage tis, t1, t2, t3, n0n1 were treated with radiotherapy alone without tumorectomy. the mean follow-up time was 15.5 years (range 5-22). the annual risk for local recurrence was 3% during the first 5 years and 1% during the following 10 years, resulting in an actuarial local control rate of 75.4% after 15 years. the risk for local recurrence was assessed in multivariate analysis and was significantly related to the size of the tumour measured on mamm ... | 2007 | 2168190 |
| presence of human papillomavirus type-6-related sequences in inverted nasal papillomas. | twenty dna samples obtained from seven cases of inverted papillomas, eight cases of nasal polyps and five cases of chronic sinusitis were investigated by southern blot hybridization for the possible presence of sequences homologous to human papillomavirus (hpv) types 6, 11, 16 and 18. hpv type-6-related dna was identified in one of the seven inverted papillomas. the restriction endonuclease cleavage patterns showed that this latter dna is a new subtype of hpv type 6 dna. in the other six papillo ... | 1990 | 2168185 |
| stabilization of glucose transporter mrna by insulin/igf-1 and glucose deprivation. | chronic exposure of l6 myocytes to insulin/igf-1 or glucose deprivation results in an increase in the level of brain-type glucose transporter (glut1) mrna. we have investigated the effects of insulin and glucose deprivation on glut1 mrna stability. the half-life of glut1 mrna in control cells was 2-2.5 h. insulin increased glut1 mrna levels by 5- to 10-fold, and glut1 mrna half-life and transcription by 2-fold. glucose deprivation increased glut1 mrna level by 2- to 4-fold and half-life by 2-fol ... | 2007 | 2168170 |
| kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid. | the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ... | 1990 | 2168160 |
| subunit analysis of bovine cytochrome c oxidase by reverse phase high performance liquid chromatography. | bovine cytochrome c oxidase subunits were separated by reverse phase high performance liquid chromatography using a c4 column eluted with water and an acetonitrile gradient, both containing 0.1% trifluoroacetic acid. subunits i and iii precipitated in this solvent and could not be analyzed; the remaining eleven subunits were dissociated, denatured, soluble and could be resolved by elution from the column. the protein subunit eluting in each chromatographic peak was identified by a combination of ... | 1990 | 2168156 |
| induction of ornithine decarboxylase mrna in cerebral cortex in response to lesion of nucleus basalis. | 1990 | 2164982 | |
| characterization of the hlyb gene and its role in the production of the el tor haemolysin of vibrio cholerae o1. | el tor strains of vibrio cholerae are capable of producing a haemolysin which they actively secrete into the growth medium. this requires translation to produce the protein at the surface of the cytoplasmic membrane and translocation across this membrane, the periplasmic space and the outer membrane. the mechanism by which this occurs is poorly understood. in addition to the structural gene for the haemolysin (hlya), we have cloned a second adjacent gene, hlyb. by site-directed mutagenesis, spec ... | 1990 | 2162464 |
| future vaccines against enteric pathogens. | a small number of bacterial agents, including enterotoxigenic escherichia coli (etec), enteropathogenic e. coli (epec), shigella and vibrio cholerae 01 and one virus, rotavirus, combine to cause a major proportion of the diarrheal illness of public health importance worldwide. salmonella typhi is by far the major cause of enteric fever. attempts to develop safe, practical, and effective vaccines against these agents are under way. examples of vaccine candidates include live oral vaccines against ... | 1990 | 2155260 |
| comparative studies of azithromycin in skin and soft-tissue infections and sexually transmitted infections by neisseria and chlamydia species. | two open, randomized, single centre studies have investigated the efficacy and safety of azithromycin (cp-62,993) in the treatment of infections by azithromycin-sensitive pathogens: (a) acute bacterial infections of skin or soft tissue (compared with erythromycin; n = 82); and (b) urethritis and/or cervicitis caused by neisseria gonorrhoeae and/or chlamydia trachomatis (compared with doxycycline; n = 108). in study a, azithromycin was administered to 42 patients for five days at a dosage of 250 ... | 1990 | 2154430 |
| interaction of cholera toxin with cloned human goblet cells in monolayer culture. | exposure of the intestinal mucosa to vibrio cholerae enterotoxin (ct) results in mucus secretion from intestinal goblet cells. on the other hand, there is evidence that elevation of intracellular adenosine 3',5'-cyclic monophosphate levels is not sufficient to induce rapid mucin secretion. to determine whether ct has direct effects on human goblet cells and whether ct alone can elicit rapid exocytosis of apical mucin granules, purified ct was applied to monolayer cultures of well-differentiated ... | 1990 | 2154122 |
| identification of an iron-regulated virulence determinant in vibrio cholerae, using tnphoa mutagenesis. | several virulence determinants of bacteria are regulated by the concentration of iron in the medium, with increased expression occurring under low-iron conditions. iron-regulated virulence factors have not been previously described in vibrio cholerae. we used the transposon vector tn5 is50l::phoa (tnphoa) to obtain insertion mutations in iron-regulated genes of v. cholerae 0395. one strain, carrying an insertion mutation in iron-regulated gene irga, had reduced virulence in an animal model and h ... | 1990 | 2152889 |
| [sensitivity of enteropathogenic bacteria to furazolidone]. | the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ... | 1990 | 2152485 |
| persistence of anti-hbs and protective efficacy after administration of hepatitis b vaccine. | to evaluate the effect of hepatitis b vaccine on the persistence of anti-hbs and its efficacy in preventing hepatitis type b, anti-hbs and anti-hbc levels were studied over a period of 5.5 years. plasma-derived hepatitis b vaccine, containing 20 micrograms of hbsag protein, was injected subcutaneously in 122 healthy medical staff members, followed by two identical injections 1 and 6 months later. anti-hbs and anti-hbc levels were then measured by radio-immunoassay. the anti-hbs titres were expre ... | 2007 | 2151729 |
| a new 12-lead pediatric ecg interpretation program. | 2008 | 2151043 | |
| immunizing against whooping cough. | 2006 | 2150961 | |
| [the epidemiological aspects of the importation of cholera into the territory of the uzbek ssr in recent years]. | 1990 | 2150142 | |
| serum lipids and lipoproteins in type 2 diabetic patients with persistent microalbuminuria. | to investigate whether persistent microalbuminuria is related to altered levels of both lipids and apolipoproteins in type 2 diabetes mellitus serum total-cholesterol, triglycerides, hdl-cholesterol, ldl-cholesterol, apolipoprotein a-i, and apolipoprotein b were measured by standard methods in a group of type 2 diabetic patients affected by persistent microalbuminuria (albumin excretion rate (aer) 20-200 micrograms min-1) as compared with a group of sex- and age-matched non-microalbuminuric pati ... | 1998 | 2148134 |
| preparation and characterization of anti-paroxetine antibodies. | 6-nitroparoxetine was synthesized and reduced to 6-aminoparoxetine. after coupling to glutaraldehyde at the 6-position and to bovine serum albumin, the resulting schiff's base was further reduced into an amino-derivative which served as the antigen. anti-paroxetine antibodies were raised against this antigen in rabbits and the anti-paroxetine igg purified by protein a affinity chromatography. the anti-paroxetine igg demonstrated high specificity towards paroxetine and 6-nitroparoxetine without s ... | 1990 | 2147049 |
| [the epidemiological importance of vibrio cholerae isolated from different ecological systems]. | the analysis of the data on the isolation of v. cholerae from different ecological systems indicates that v. eltor do not constantly inhibit the rivers and sea at the territory under control. hemolytically active v. cholerae without the vct gene, found to be faintly virulent and avirulent when studied on suckling rabbits used as a model and when evaluated by the complex method, show no tendency towards epidemic spread in the presence of conditions for the realization of the transmission of vibri ... | 1990 | 2146847 |
| iron-binding compounds and related outer membrane proteins in vibrio cholerae non-o1 strains from aquatic environments. | a total of 156 strains of vibrio cholerae non-o1 from aquatic origins were examined for the presence of iron uptake mechanisms and compared with o1 strains and other vibrio species. all non-o1 strains were able to grow in iron-limiting conditions, with mics of ethylenediaminedi (o-hydroxyphenylacetic acid) ranging from 20 microm to 2 mm. the production of siderophores was demonstrated by growth in chrome azurol s agar and cross-feeding assays. all strains produced phenolate-type compounds, as as ... | 1990 | 2144956 |
| [comparative study of cholera phages isolated from the water of open reservoirs and lysogenic strains of vibrio el-tor]. | 1990 | 2144501 | |
| stimulation of a human erythrocyte membrane atpase by glutathione conjugates. | an atp-dependent transport process for s-(2,4-dinitrophenyl) glutathione (dnp-sg) mediated by a novel atpase designated as dnp-sg atpase has been demonstrated in human erythrocytes (labelle et al., febs lett. 228, 53-51, 1988). in order to investigate whether the dnp-sg atpase system represents a generalized mechanism for the transport of xenobiotic conjugates of glutathione (gsh), stimulation of this atpase by different gsh conjugates was studied in membrane vesicles prepared from human erythro ... | 1990 | 2143605 |
| influence of chronic alterations of salt intake and aging on the kinetic of red cell na+ and k+ transport in sprague-dawley rats. | the kinetics of na+ and k+ (rb)+ transport mediated by the na(+)-k+ pump and na(+)-k+ cotransport system (assessed as a function of rb+o and na+i) as well as the magnitude of cation leaks were determined in red cells of young male rats subjected to chronic salt deprivation or salt loading (0.1% and 8% nacl diet). these salt intake alterations induced moderate kinetic changes of the na(+)-k+ pump which did not result in significant changes of ouabain-sensitive (os) rb+ uptake or na+ net extrusion ... | 1990 | 2142786 |
| [dementia disorders among older people; description of disease conditions and odontologic consequences]. | 1994 | 2142599 | |
| serum steroid hormones and pituitary function in female epileptic patients during carbamazepine therapy. | ten regularly menstruating women with epilepsy were studied in a 12-month prospective follow-up study to evaluate the short-term effects of carbamazepine (cbz) on serum sex hormone balance and pituitary function. thirteen female epilepsy patients receiving long-term cbz monotherapy (mean medication duration 5.3 years) were also studied. controls were 17 regularly menstruating healthy volunteers. untreated patients had higher free testosterone (ft) and luteinizing hormone (lh) serum concentration ... | 1993 | 2142450 |
| 3 processing of histone rna precursors. | 2007 | 2141913 | |
| contribution of u1 snrna structural domains to u1 snrnp function. | 1990 | 2141898 | |
| effect of atropine on plasma atrial natriuretic peptide concentration in healthy subjects. | this work shows that an intravenous infusion of atropine increases the concentration of atrial natriuretic peptide (anp) in the plasma of healthy subjects. the effect is discussed in reference to the heart rate and the role of the autonomic nervous system in the control of anp secretion by atrial cardiocytes. | 1998 | 2141774 |
| [current knowledge of the immunogenetics of spondylarthritis]. | 1998 | 2140156 | |
| analysis of cis-acting requirements of the rh3 and rh4 genes reveals a bipartite organization to rhodopsin promoters in drosophila melanogaster. | the rhodopsin genes of drosophila melanogaster are expressed in nonoverlapping subsets of photoreceptor cells within the insect visual system. two of these genes, rh3 and rh4, are known to display complementary expression patterns in the uv-sensitive r7 photoreceptor cell population of the compound eye. in addition, we find that rh3 is expressed in a small group of paired r7 and r8 photoreceptor cells at the dorsal eye margin that are apparently specialized for the detection of polarized light. ... | 2007 | 2140105 |
| strikingly low atpase activities in flagellar axonemes of a chlamydomonas mutant missing outer dynein arms. | the atpase activities in chlamydomonas axonemes were compared between wild type and a mutant (oda) that lacks entire outer dynein arms, at various ionic strengths and ph values, and in the presence of different concentrations of high-molecular-mass dextran. over a 0-0.2 m kcl concentration range, the atpase activity of oda axonemes was found to be 5-12 times lower than that of the wild-type axonemes. the low activity in oda is surprising since outer arm-depleted axonemes of sea urchin sperm have ... | 2007 | 2140096 |
| strikingly low atpase activities in flagellar axonemes of a chlamydomonas mutant missing outer dynein arms. | the atpase activities in chlamydomonas axonemes were compared between wild type and a mutant (oda) that lacks entire outer dynein arms, at various ionic strengths and ph values, and in the presence of different concentrations of high-molecular-mass dextran. over a 0-0.2 m kcl concentration range, the atpase activity of oda axonemes was found to be 5-12 times lower than that of the wild-type axonemes. the low activity in oda is surprising since outer arm-depleted axonemes of sea urchin sperm have ... | 2007 | 2140096 |
| studies of experimental hosiery in diabetic neuropathic patients with high foot pressures. | high plantar pressures and painless trauma are associated with the development of foot ulcers in diabetic patients. padded hosiery has been reported to reduce plantar pressures in patients at risk of ulceration. using the optical pedobarograph we have studied 10 patients who regularly wore experimental padded hosiery for 6 months. the hosiery continued to provide substantial and significant reduction in peak forefoot pressures at 3 months (mean reduction 15.5%, p less than 0.01) and 6 months (17 ... | 2007 | 2140084 |
| dna damage and prophage induction and toxicity of nitrofurantoin in escherichia coli and vibrio cholerae cells. | repair-deficient and repair-proficient strains of e. coli k12 were sensitive to nitrofurantoin treatment to varying degrees with the double mutant strain (uvra 6, reca 13) being most sensitive. ultraviolet absorption data and thermal chromatography through a hydroxyapatite column revealed that nitrofurantoin treatment of v. cholerae strain ogawa 154 produced a maximal amount of 55% reversibly bihelical dna at a nitrofurantoin dose of 120 micrograms/ml/h, which indicated the formation of inter-st ... | 1990 | 2139919 |
| activation of the e2f transcription factor in adenovirus-infected cells involves e1a-dependent stimulation of dna-binding activity and induction of cooperative binding mediated by an e4 gene product. | previous experiments have demonstrated that the dna-binding activity of the e2f transcription factor is increased upon adenovirus infection and that both the e1a and e4 genes are required for activation. in this study, we demonstrated that this enhanced binding of e2f to the e2 promoter is the result of two events. (i) there is stimulation of the dna-binding activity of the e2f factor; this stimulation is e1a dependent but independent of e4. (ii) there is also induction of a stabilized interacti ... | 1990 | 2139893 |
| curriculum guidelines for training general practice residents to treat the person with a handicap. | 1997 | 2139881 | |
| lovastatin, pravastatin, and serum lipoprotein(a) | 1997 | 2139768 | |
| the glycosyl phosphatidylinositol-linked fc gamma riiipmn mediates transmembrane signaling events distinct from fc gamma rii. | to investigate the ability of fcgammariii(pmn), the gpi-anchored isoform of fcgammariii (cd16) in polymorphonuclear leukocytes (pmn), to mediate transmembrane signaling events, we measured changes in membrane potential with dioc(5) and in intracellular calcium with indo-1. fcgammar were ligated by anti-fcgammariii mab 3g8 (igg and fab), anti-fcgammarii mab iv.3 (igg and fab), and human igg aggregates. cell bound mabs were also crosslinked by goat f(ab')(2) anti-mouse igg. 3g8 igg elicited a rapi ... | 1990 | 2139101 |
| serious adverse drug reactions to pyrimethamine-sulphadoxine, pyrimethamine-dapsone and to amodiaquine in britain. | all reports of adverse reactions with pyrimethamine-sulphadoxine (fansidar), pyrimethamine-dapsone (maloprim), and amodiaquine spontaneously reported through the uk national post-marketing system were reviewed. retrospective reporting rates of serious reactions associated with these drugs were analysed using prescription data from the department of health, derived from the prescription pricing authority, and relevant pharmaceutical companies. whilst interpretation of these data requires caution, ... | 1990 | 2138674 |
| ca2(+)-dependent membrane-binding proteins in normal erythrocytes and erythrocytes from patients with chronic myelogenous leukemia. | cytosolic and membrane fractions of human erythrocytes were probed with antisera raised against several members of the annexin family of ca2(+)-dependent phospholipid/membrane-binding proteins. one of the antisera, that against the 67 kd calcimedin, identified erythrocyte polypeptides of molecular weights 48 and 67 kd, which were found in the cytoplasm when normal erythrocytes were lysed in the presence of egta but on the membrane when lysis buffers contained ca2+. in contrast, membranes of eryt ... | 1990 | 2138037 |
| surfaces of murine lymphocyte subsets differ in sialylation states and antigen distribution of a major n-linked penultimate saccharide structure. | rat liver beta-galactoside alpha-2,6-sialyltransferase and vibrio cholerae sialidase were used with cytidine-5'-monophospho-n-acetyl-[3h]neuraminic acid (cmp-[3h]neuac) to specifically probe the distribution and sialylation state of gal beta 1-4glcnac residues on n-linked saccharides on the surfaces of murine lymphocytes. the relative extent of exogenous sialyltransferase-mediated sialylation (per cellular protein) was thymocytes greater than t-cells greater than t-cell lymphoma (el-4) greater t ... | 1990 | 2137033 |
| kinetic analysis of 3-quinuclidinyl 4-[125i]iodobenzilate transport and specific binding to muscarinic acetylcholine receptor in rat brain in vivo: implications for human studies. | radioiodinated r- and s-quinuclidinyl derivatives of rs-benzilate (r- and s-125iqnb) have been synthesized for quantitative evaluation of muscarinic acetylcholine receptor binding in vivo. two sets of experiments were performed in rats. the first involved determining the metabolite-corrected blood concentration and tissue distribution of tracer r-iqnb (active enantiomer) and s-iqnb (inactive enantiomer) in brain 1 min to 26 h after intravenous injection. the second involved the measurement of br ... | 1990 | 2134838 |
| the expressed empathy of psychiatric nursing staff. | 1982 | 2134204 | |
| 1,25 dihydroxycholecalciferol and the genesis of hypocalcaemia in magnesium-deficient chicks. | the role of 1,25 dihydroxycholecalciferol (1,25 dhcc) in the genesis of hypocalcaemia of magnesium (mg) deficiency was studied in chicks fed control or mg-deficient diets with varying calcium (ca) content. in one study, ca and mg deficiencies were induced simultaneously whereas in a second study, exposure to low dietary ca followed induction of mg deficiency. in both studies, mg-deficient chicks fed adequate dietary ca were hypocalcaemic yet had normal circulating 1,25 dhcc, whereas mg-deficient ... | 1990 | 2132748 |
| htlv-i infection in britain. | 1997 | 2132563 | |
| histomorphological criteria for prognosis in breast cancer. | one hundred cases of carcinoma breast were analysed with regard to histological features and clinical follow-up. bad prognostic factors included tumour size larger than 5cms; absent or minimal intraduct component in the tumour and absent or minimal periductal elastosis. conversely significant intraduct component in the tumour and periductal elastosis were associated with better survival. degree of lymph nodal involvement did not influence the outcome. one reason for this unexpected finding could ... | 1997 | 2132500 |
| [presence of salmonella in the northwest shore of the l'albufera de valencia]. | for the purpose of ascertaining the amount of pollution reaching the large salt lagoon in valencia from its northwest side, 19 river-beds flowing into it have been studied. for each sample, the presence of markers indicating fecal pollution and the presence or absence of pathogens; salmonella, vibrio cholerae and shigella have been evaluated. for each river-bed, a fecal pollution index has been calculated, this index revealing a positive correlation (p = 0.03) with the isolating of salmonella. s ... | 1990 | 2131632 |
| [evaluation of systolic and diastolic left ventricular function in acute myocardial infarction by aortic echogram]. | a noninvasive method for the measurement of the cardiac output and diastolic ventricular function was evaluated by aortic echogram in a group of patients with acute myocardial infarction (ami). the following parameters were employed: average of initial and late aortic cusp separation (acs), left ventricular ejection time (ep) and amplitude of aortic posterior wall during ejection (aa). the following formula was used: cardiac output (co) = (acs x ep) x 100 + aa x hr. diastolic ventricular functio ... | 1967 | 2130226 |
| [findings of intestinal parasites in relation to age in school children from guca and the surrounding region]. | 1972 | 2130016 | |
| [results of triennial screening of a mountain community school population in ussl n. 42]. | the paper reports data regarding dental caries in deciduous and permanent teeth in a sample population of 1015 pupils aged between 5 and 15, resident in the mountain community of the chisone and germanasca valleys. the dmf index was significantly high, especially if one takes into account that the partial findings referring to '89 are virtually unchanged in comparison to '87. | 1986 | 2129585 |
| cyclosporin-induced renal magnesium leak in renal transplant patients. | renal transplant patients (n = 116) attending an outpatients clinic were screened for hypomagnesaemia. no azathioprine-treated patients (n = 46) but 24% (17 of 70) of the cyclosporin treated patients were hypomagnesaemic. the hypomagnesaemia in all cases was associated with a renal magnesium leak. this leak did not correlate with plasma bicarbonate, urate, calcium, cyclosporin or creatinine concentrations, nor did it correlate with the use of loop diuretics or duration of the transplant. a renal ... | 1984 | 2129356 |
| telomere and centromere dna are associated with the cores of meiotic prophase chromosomes. | mouse (mus musculus) whole-mount, surface-spread, meiotic prophase chromosomes have an axial core structure, the synaptonemal complex, sc, from which extend chromatin loops. this arrangement permits a novel approach to the analysis of chromosome structure. using in situ hybridization, the types of dna sequences preferentially associated with the sc and the types located primarily in the chromatin loops can be determined. with biotinylated probes, detected by avidin conjugated to fitc, we present ... | 1990 | 2129288 |
| expression of toxr, the transcriptional activator of the virulence factors in vibrio cholerae, is modulated by the heat shock response. | the toxr gene of vibrio cholerae encodes a transmembrane, dna-binding protein that positively controls transcription of the genes for cholera toxin, tcp pili, and other proteins important in cholera pathogenesis. nucleotide sequence analysis of the toxr upstream region has revealed that the heat shock gene htpg, encoding the bacterial homologue of the eukaryotic hsp90 protein, was located immediately upstream and was divergently transcribed from toxr. using lacz transcriptional fusions, we have ... | 1990 | 2124707 |
| [purification and various properties of vibrio cholerae non-01 cytolysin]. | a new method for isolation and purification of vibrio cholerae non-01 cytolysin has been elaborated. it includes the steps of concentration by ammonium sulphate, ion exchange chromatography on de-52-cellulose, gel filtration via ultragel asa-44 and chromatography on mono q. cytolysin is shown to be a 60 kd and pi 6.2 protein. it is not inactivated by thiol-restoring agents and induces the production of precipitating antibodies. in contrast to choleragen the protein cytolytically affects erythroc ... | 1990 | 2123963 |
| comparison of the vibrio cholerae hemagglutinin/protease and the pseudomonas aeruginosa elastase. | the soluble hemagglutinin/protease (ha/protease) produced by vibrio cholerae and the elastase of pseudomonas aeruginosa are both zinc/calcium-dependent proteases. in the present study the two enzymes are compared immunologically and functionally. the n-terminal amino acid sequences of the proteins had 65% identity within the first 20 amino acids. polyclonal antisera against each purified protein recognized the enzyme of the other species in enzyme-linked immunosorbent assay, checkerboard immunob ... | 1990 | 2123831 |
| nonsurgical bypass of malignant duodenal and biliary obstruction. | 2007 | 2121584 | |
| polarized secretion of diamine oxidase by intestinal epithelial cells and its stimulation by heparin. | the caco-2 cells have been used as a model system to study the pathways of diamine oxidase secretion by the intestinal epithelium. when grown in transwell filter chamber devices, the polarized cell monolayers released the enzyme preferentially into the basal chamber. heparin (1-10 usp u/ml) rapidly induced a marked stimulation of enzyme secretion only when in contact with the basolateral cell membrane, where high affinity binding sites for [3h]heparin were also exclusively located. among the oth ... | 1990 | 2121582 |
| properties of a hemolysin related to the thermostable direct hemolysin produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus. | a hemolytic toxin (vp-trh) produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus was further characterized. the purified vp-trh showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (vp-tdh), a pathogenic toxin produced by kanagawa phenomen ... | 1990 | 2118824 |
| effect of partial discectomy on the stability of the lumbar spine. a study of kinematics. | the effect on the stability of the lumbar motion segment, as affected by partial discectomy, was investigated using three-dimensional motion measurement technique. eight functional spinal units from fresh cadaver lumbar spines were potted and clinically relevant loads applied through a special loading frame attached rigidly to the top vertebra of the specimen. partial discectomy was performed on the right side of the specimen. the three-dimensional motions of each lumbar motion segment, before a ... | 2007 | 2118056 |
| a single pre-b cell can give rise to antigen-specific b cells that utilize distinct immunoglobulin gene rearrangements. | a group of hybridomas that express antibodies with related specificities for the influenza virus hemagglutinin (ha), that represent b cells that were the clonal progeny of a single pre-b cell, and that utilized distinct l chain gene rearrangements have been characterized. the clonal relationship was established by the sharing of h chain gene rearrangements at both the productive and the nonproductive alleles. among these hybridomas, one group had rearranged only one of its kappa alleles, having ... | 1990 | 2117635 |
| a single pre-b cell can give rise to antigen-specific b cells that utilize distinct immunoglobulin gene rearrangements. | a group of hybridomas that express antibodies with related specificities for the influenza virus hemagglutinin (ha), that represent b cells that were the clonal progeny of a single pre-b cell, and that utilized distinct l chain gene rearrangements have been characterized. the clonal relationship was established by the sharing of h chain gene rearrangements at both the productive and the nonproductive alleles. among these hybridomas, one group had rearranged only one of its kappa alleles, having ... | 1990 | 2117635 |
| imaging of chronic renal infections. | 2007 | 2117344 | |
| limited proteolysis of beta 2-microglobulin at lys-58 by complement component c1s. | we have now demonstrated that activated complement component c1s cleaves beta 2-microglobulin at the position identical to that at which beta 2-microglobulin is cleaved in serum of patients suffering from lung cancer. the main cleavage is in the disulphide loop c-terminal to lys-58, generating a modified form of beta 2-microglobulin with a two-chain structure. the c-terminal lys-58 in the a chain is highly susceptible to removal by a carboxypeptidase-b-like activity causing the formation of des- ... | 2007 | 2110898 |
| closely related glycosylation patterns of recombinant human il-2 expressed in a cho cell line and natural il-2. | we report here the study of the glycosylation pattern of human recombinant (r) il2 expressed in a chinese hamster ovary (cho) cell line. the human ril2 secreted by this high-producing recombinant cho cell line was metabolically radiolabelled with [35s]-methionine, or with [3h]-glucosamine and [3h]-galactose, purified to homogeneity, and then characterized. the electrophoretic analysis of the [35s]-methionine-labelled proteins present in the culture medium of the cho cell line showed that the ril ... | 1990 | 2109157 |
| long-term persistence of toxigenic vibrio cholerae 01 in the mucilaginous sheath of a blue-green alga, anabaena variabilis. | cholera epidemics occur twice a year in the endemic area of bangladesh. vibrio cholerae 01 can be isolated from the environment only during the epidemics and the question of possible interepidemic environmental reservoirs of v. cholerae remains open. the present laboratory-based studies investigate the role of an aquatic alga, anabaena variabilis, as a possible reservoir. persistence of v. cholerae inside the mucilaginous sheath of a. variabilis was observed by phase-contrast and fluorescent mic ... | 1990 | 2109096 |
| neuroblastoma differentiation involves the expression of two isoforms of the alpha-subunit of go. | the regulation of gtp-binding proteins (g proteins) was examined during the course of differentiation of neuroblastoma n1e-115 cells. n1e-115 cell membranes possess three bordetella pertussis toxin (ptx) substrates assigned to alpha-subunits (g alpha) of go (a g protein of unknown function) and "gi (a g protein inhibitory to adenylate cyclase)-like" proteins and one substrate of vibrio cholerae toxin corresponding to an alpha-subunit of gs (a g protein stimulatory to adenylate cyclase). in undif ... | 1990 | 2107277 |
| a study of v. cholerae strains isolated in bombay. | of 935 faecal samples studied over a period of one year, v. cholerae 01 was isolated from 102 samples (10.9%). all the strains were found to be e1 tor ogawa. the strains belonging to the phage types 2 and 4 were encountered in our study, type 2 being the highest (76.5%). the sensitivity pattern of all strains to the commonly used antibiotics was determined. strains sensitive to gentamicin (92.2%), nalidixic acid (85.3%), kanamycin (83.3%), cotrimoxazole (80.4%) and chloramphenicol (75.5%) were o ... | 1990 | 2102910 |
| delivery of the cholera toxin b subunit by using a recombinant yersinia enterocolitica strain as a live oral carrier. | the gene ctxb encoding the cholera toxin b subunit was subcloned to design its production by yersinia enterocolitica. it was joined in two ways to yoph, a gene of the virulence plasmid pyv specific to this genus. this gene encodes one of the major yop proteins (yoph) secreted by bacteria incubated at 37 degrees c in a ca(2+)-deprived medium. in a first construction, an operon fusion was obtained between ctxb and yoph so that ct-b and a truncated yoph protein were produced. the recombinant ct-b f ... | 1990 | 2101483 |
| recombinant attenuated vibrio cholerae strains used as live oral vaccines. | although great strides have been made in the development of recombinant attenuated vibrio cholerae vaccine strains, the task has not been as simple as once imagined. the initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as cvd103-hgr, are well-tolerated, immunogenic and protective after a single dose. in addition, this strain carries a selectable marker to distinguish it from wild strains and has been evaluated in a practical, lyophilized formulation ... | 1990 | 2101480 |
| new attenuated derivatives of vibrio cholerae. | 1990 | 2101479 | |
| construction of an invertible dna segment for improved antigen expression by a hybrid salmonella vaccine strain. | 1990 | 2101464 | |
| effectiveness of oral rehydration salt solution (ors) in reduction of death during cholera epidemic. | an extensive outbreak of acute gastroenteritis involving all age group of patients occurred during july-september, 1978 in the central district of manipur state. a total of 4469 cases occurred during the period. 45.7% of diarrhoea cases sampled and 47.6% of water samples collected from rivers were found to be positive for v.cholerae biotype eitor. case fatality rate in this epidemic was exceptionally low (0.8%) which was attributed to the early domiciliary use of oral rehydration salt solution ( ... | 1990 | 2101392 |
| etiological agents of diarrhoea. | two decades of research have established newer pathogens and techniques in establishing several organisms of diarrhoeal diseases as aetiological agents. it is now possible to detect an agent in 80% of the situation of diarrhoea in a standard laboratory. the brief review describes the list of pathogens, their diagnostic techniques with short description on clinical and epidemiological status. | 1990 | 2101388 |
| management of acute diarrhoea. | before 1970, laboratory staff could not only identify the causative organism of acute diarrhea in 20% of cases, but in 1990, they could identify it in 80% of cases. these organisms are either bacteria, virus, or parasites. the bacteria include enterotoxigenic bacteria (vibrio cholerae, enterotoxigenic escherichia coli, clostridium perfringens, and staphylococcus aureus) and enteroinvasive bacteria (campylobacter jejuni, c. coli, and salmonella and shigella species). the leading cause of death ... | 1990 | 2101385 |