Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [standardization of an indirect enzyme-linked immunosorbent assay (elisa) for demonstration of antibodies to chicken bronchitis virus]. | 1981 | 6282010 | |
| effects of three avian respiratory viruses on pathogenicity in tracheal organ cultures. | 1981 | 6279947 | |
| use of a constant-virus diluting-serum microneutralization technique for the detection and quantification of infectious bronchitis virus antibodies. | a constant-virus diluting-serum microneutralization test (cvmnt) for avian infectious bronchits virus (ibv) was evaluated for both reliability and repeatability. the virus used in the assay was a chick kidney (ck) cell-adapted strain, the beaudette strain (ibv 42). sera tested were from 24-week-old broiler-breeder chickens that had been vaccinated 3 times from a combination vaccine of newcastle disease virus (ndv) and ibv. test results were not repeatable or comparable when the same sera were te ... | 1981 | 6279070 |
| diagnosis of infectious bronchitis (ib) by examination of tracheal mucus for ib-precipitating antigens. | the use of tracheal exudates for detecting precipitating infectious bronchitis (ib) antigen by the agar-gel precipitin (agp) technique is described. of 47 experimentally infected tracheas, 24 gave positive precipitin lines between the second and 12th day postinfection; 9 out of 18 tracheas from birds from natural outbreaks of ib were also positive. tests can be read within 18-24 hr. for best results, several antisera or an antiserum at various concentrations should be used. | 1981 | 6279064 |
| the avian coronavirus multiplication strategy. | 1981 | 6278881 | |
| oligonucleotide fingerprinting of the rna of different strains of infectious bronchitis virus. | 11 distinct oligonucleotide fingerprints were obtained in studies of the rna of 13 isolates of ibv. different serotypes had distinct fingerprints, but so did varieties within a serotype; allowing a greater degree of strain differentiation than was previously possible. some conclusions can be drawn from the fingerprints concerning theories of origin and spread of ibv. | 1981 | 6278877 |
| plaque formation by avian infectious bronchitis virus in primary chick embryo fibroblast cells in the presence of trypsin. | ten strains of avian infectious bronchitis virus (ibv) were titrated as plaque-forming units in primary chick embryo fibroblast cells. in the absence of trypsin, plaques were only formed by beaudette-42 and iowa-609 strains. when trypsin was incorporated in the overlay medium of cell monolayers, all the ibv strains tested produced plaques within 4 days after inoculation. incorporation of 20-40 microgram of trypsin per ml of the overlay medium seemed to be suitable for plaque formation of ibv. a ... | 1981 | 6275820 |
| cell mediated and humoral immune response of chickens to live infectious bronchitis vaccines. | a lymphocyte transformation microassay was used to study cell-mediated immune (cmi) responses in chickens following inoculation with live infectious bronchitis virus vaccines h 120 and h 52 and challenge with massachusetts-41 (m-41). humoral immunity was monitored for comparison, using haemagglutination inhibition (hi) microassay. specific stimulation of infectious bronchitis virus sensitised lymphocytes was demonstrated by their lack of response to mycoplasma synoviae and newcastle disease viru ... | 1981 | 6275477 |
| effectiveness of maternal antibody against challenge with infectious bronchitis viruses. | 1981 | 6274299 | |
| detection and quantification of antibodies to infectious bronchitis virus by enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay (elisa) for detecting and quantifying antibodies to infectious bronchitis virus (ibv) is described. purified antigen, prepared on sucrose density gradients, was required to decrease the nonspecific background, and saline was found to be superior to bicarbonate buffer for coating the cuvettes with antigen. the sensitivity of the test in measuring antiserum titers could be altered greatly and linearly by adjusting the protein content of the antigen. the elisa w ... | 1981 | 6274297 |
| serologic and cross-protection studies with several infectious bronchitis virus isolates from delmarva-reared broiler chickens. | five isolates, from broiler-type chickens, capable of causing acute respiratory disease were identified as infectious bronchitis virus (ibv). the agar-gel precipitin (agp) test was effective in identifying the isolates as ibv. virus-neutralization (vn) and cross-challenge tests indicated two of the isolates to be massachusetts types and one to be a jmk type. the remaining isolates were different from strains normally encountered in chickens reared on the delmarva peninsula. one isolate, although ... | 1981 | 6274294 |
| cross-immunity in chickens using seven isolates of avian infectious bronchitis virus. | groups of 75 chickens were each infected with one of 7 isolates of infectious bronchitis virus (mass 41, holland 52, se 17, ark 99, clark 333, jmk, and florida). following recovery, they were challenged along with susceptible controls to the homologous and 6 heterologous isolates. cross-immunity was determined by virus recovery 4 or 5 days post-challenge. challenge with the homologous isolate resulted in 90-100% protection. challenge with heterologous isolates gave variable results and an overa ... | 1981 | 6274293 |
| influence of temperature of incubation on chicken embryo tracheal organ cultures and chick embryos infected with strains of avian infectious bronchitis virus. | the ciliostatic and embryo lethal effect of four strains of avian infectious bronchitis virus (ibv) were observed at two different incubation temperatures. in organ cultures, nephrosis-producing ibv strains (gn-2 and m-41) were slightly more ciliostatic and respiratory strains (beaudette and kita-1/tokushima) less ciliostatic at 40.5 degrees c than at 37 degrees c. mortality of chicken embryos infected with the gn-2, m-41 and kita-1/tokushima strains was greater at 40.5 degrees c than at 37 degr ... | 1981 | 6273983 |
| polypeptides of infectious bronchitis virus. i. polypeptides of the virion. | infectious bronchitis virus (ibv), strain beaudette, grown in cultured cells contained five structural proteins with apparent mol. wt. of 170 000 (p170), 94 000 (gp94), 50 000 (pp50) 30 000 (gp30) and 26 000 (p26). both gp94 and gp30 are glycopeptides since they were labelled with [3h]glucosamine. the only phosphorylated polypeptide was pp50, and both it and gp94 were occasionally resolved into two bands. two other polypeptides with mol. wt. of 28 000 (p28) and 14 000 (p14) were sometimes associ ... | 1981 | 6271900 |
| structural polypeptides of coronavirus ibv. | avian infectious bronchitis virus (ibv) was grown and radiolabelled with 35s-methionine, 3h-leucine and 3h-glucosamine in de-embryonated chicken eggs. approximately 12 different polypeptides were clearly detected by sds-polyacrylamide gel electrophoresis of virus preparations. growth of ibv in chorioallantoic membrane cells labelled with 35s-methionine indicated that most of these polypeptides, and additional ones, some of which were glycosylated, were host components. five polypeptides appeared ... | 1981 | 6268743 |
| ribonucleoprotein of avian infectious bronchitis virus. | the ribonucleoprotein (rnp) of avian infectious bronchitis virus (ibv) was examined by electron microscopy after shadowing with carbon/platinum. linear rnp strands up to 6.7 microns in length, from three ivb strains, were sensitive to both pancreatic rnase and to proteases. these strands were obtained from spontaneously disrupted complete particles but not from disrupted incomplete particles that lacked rnp. they were also released from nonidet p40-disrupted particles and could be isolated on su ... | 1981 | 6268741 |
| structural proteins and glycoproteins of infectious bronchitis virus particles labeled during growth in chick embryo cells. | seven australian infectious bronchitis viruses incorporated radioactive amino acid label during growth in two types of cell culture. from purified virions, four major polypeptides of molecular weights 91,000 (gp91), 51,000 (vp51), 25,000 (gp25) and 23,000 (vp23) were separated by page. two minor polypeptides of molecular weights 210,000 (gp210) and 185,000 (gp185) were also identified. 3h-glucosamine was incorporated during growth of virus into gp210, gp185, gp91 and gp25. bromelain digestion of ... | 1981 | 6268567 |
| pathology of experimental nephrosis-nephritis in chicks induced by infectious bronchitis virus. | 1981 | 6268535 | |
| comparison of a tissue-culture virus-neutralization test and the enzyme-linked immunosorbent assay for measurement of antibodies t infectious bronchitis. | two serological tests, the virus-neutralization (vn) test in tissue culture using a tissue-cell-adapted virus and the enzyme-linked immunosorbent assay (elisa), were compared to detect antibodies against massachusetts 41 and connecticut 46 strains of infectious bronchitis virus (ibv). the vn test was conducted in wells of microplates by the usual procedure. the two strains of ibv were adapted after 20 serial passages to induce cpe in 24 hours in chickens embryos kidney cells (cekc). the elisa te ... | 1981 | 6268048 |
| pathogenicity of two strains of mycoplasma gallisepticum in broilers. | strains f and r of mycoplasma gallisepticum (mg) were compared in two laboratory trials for their relative pathogenicity in terms of inducing airsacculitis and antibody production to mg. chickens exposed to the r strain had significantly higher incidence of air-sac lesions (p less than 0.05) and greater severity of airsacculitis than did chicks exposed to the f strain. in both trials, chickens vaccinated simultaneously with newcastle disease-infectious bronchitis vaccine and exposed to mg had mo ... | 1980 | 6268040 |
| inner structures of some coronaviruses. | when treated with formaldehyde, tween 80, sodium oleate and nonidet p-40, avian infectious bronchitis virus, porcine transmissible gastroenteritis virus, neonatal calf diarrhea coronavirus, porcine hemagglutinating encephalomyelitis virus as well as the human coronavirus show similar inner structures by negative staining. the first one is an inner membranous bag. this structure could be evaginated following treatments used and does not show the characteristic projections of coronaviruses. subseq ... | 1981 | 6266623 |
| local antibody response in avian infectious bronchitis: virus-neutralizing antibody in tracheobronchial secretions. | specific-pathogen-free chickens were tested for local antibody response after respiratory exposure to live avian infectious bronchitis virus (ibv). tracheobronchial secretions were obtained after intraocular-intratracheal vaccinations with holland and massachusetts 41 strains ibv. low levels of immunoglobulin (ig)a and igg virus-neutralizing antibodies were detected in secretions after each of two vaccinations with a moderate dose (10(4) eid50), neutralizing activity in secretions was found main ... | 1981 | 6266389 |
| immune responses of breeding chickens to trivalent oil emulsion vaccines: responses to infectious bronchitis. | three similar flocks of broiler breeder parent chickens that had been given live infections bronchitis (ib) vaccines during rearing were injected at 20 weeks of age with three different oil emulsion vaccines: a commercial monovalent newcastle disease (nd) vaccine (flock a); an experimental bivalent vaccine containing nd and infectious bursal disease (ibd) components (flock b); and an experimental trivalent vaccine containing nd, ibd and ib components (flock c). one week after vaccination 40 hens ... | 1981 | 6266125 |
| immune responses of breeding chickens to trivalent oil emulsion vaccines: responses to newcastle disease and infectious bursal disease. | bivalent newcastle disease (nd)/infectious bursal disease (ibd) and trivalent nd/ibd/infectious bronchitis (ib) inactivated oil emulsion vaccines were prepared in the laboratory and evaluated under field conditions. broiler breeder parent chickens previously vaccinated with live vaccines were inoculated with commercial monovalent nd and experimental bivalent or trivalent oil emulsion vaccines. the commercial vaccine induced a higher initial nd haemagglutination inhibition (hi) response than the ... | 1981 | 6266123 |
| oligonucleotide fingerprinting of ribonucleic acids of infectious bronchitis virus strains. | a total of 11 distinct oligonucleotide fingerprints were obtained in studies of the ribonucleic acids of 13 isolates of infectious bronchitis virus. different serotypes had distinct fingerprints, but so did varieties within a serotype, allowing a greater degree of strain differentiation than was previously possible. some conclusions can be drawn from the fingerprints concerning theories of origin and spread of infectious bronchitis virus. | 1981 | 6265376 |
| australian infectious bronchitis viruses: identification of nine subtypes by a neutralisation test. | the antigenic relationships between 17 australian infectious bronchitis viruses, including six vaccine viruses, were studied by a neutralisation test using a plaque reduction method in chick embryo kidney cell monolayers. the 17 viruses formed nine distinct subtypes. antiserum to each subtype had a high titre to viruses of the same subtype and a lower titre to viruses of different subtypes. the heterologous titres of sera varied widely. | 1981 | 6264556 |
| australian infectious bronchitis viruses: plaque formation and assay methods. | 1981 | 6264555 | |
| the disruption of infectious bronchitis virus (ibv-41 strain) with triton x-100 detergent. | 1980 | 6262334 | |
| survival of viruses in fermented edible waste material. | the survival of selected viruses in fermented edible waste material was studied to determine the feasibility of using this material as a livestock feed ingredient. seven viruses, including pseudorabies, newcastle disease, infectious canine hepatitis, avian infectious bronchitis, measles, vesicular stomatitis, and a porcine picornavirus were inoculated into a mixture of ground food waste (collected from a school lung program) containing lactobacillus acidophilus. mixtures were incubated at 5 c, 1 ... | 1981 | 6261622 |
| immune responses of chickens to ib vaccines. | 1981 | 6261436 | |
| isolation of infectious bronchitis, infectious bursal disease and avian adenoviruses from chickens with nephritis-nephrosis syndrome. | 1980 | 6255706 | |
| infectious bronchitis agar-gel precipitin test--use of infected allantoic fluid as antigen. | the use of infected allantoic fluid (af) as precipitating infectious bronchitis (ib) antigen was investigated. the results show that unconcentrated af harvested at the right time can be a very satisfactory precipitating ib antigen. with the majority of the virus strains used, unconcentrated af, harvested 68 hours postinoculation (pi), showed more precipitating activity than that harvested at 20 or 120 hours pi. if further antigen concentration is required, dialysis with polyethyleneglycol mw 20, ... | 1980 | 6254493 |
| avian infectious bronchitis in specific-pathogen-free chickens: quantitation of serum immunoglobulins by electroimmunoassay. | immunoglobulin (ig) levels in serums of individual 6-week-old specific-pathogen-free (spf) chickens differed markedly. quantitation by electroimmunoassay showed that the igg level was quite low at 6 weeks of age (1.3 +/- 0.67 mg/ml), increased gradually with maturity, and by 16-18 weeks of age was 2.6 +/- 1.5 mg/ml. igm levels were less variable during the same observation period (6-18 weeks, 2.4 +/- 0.50 to 2.2 +/- 0.86 mg/ml). iga was high initially and decreased with age (6-18 weeks, 0.74 +/- ... | 1980 | 6254487 |
| freeze-dried infectious bronchitis haemagglutinating antigen. | 1980 | 6254230 | |
| performance of 3 successive generations of specified-pathogenfree chickens maintained as a closed flock. | no antibodies against salmonella pullorum, mycoplasma gallisepticum, mycoplasma synoviae, haemophilus gallinarum, fowl pox virus, marek's disease virus, herpes virus of turkey, infectious laryngotracheitis virus, avian adenovirus, avian reovirus, infectious bursal disease virus, reticuloendotheliosis virus, avian leukosis virus, avian encephalomyelitis virus and newcastle disease virus were detectable in the sera obtained from these chickens in 3 generations at various ages. antibodies against i ... | 1980 | 6253742 |
| coronavirus multiplication strategy. ii. mapping the avian infectious bronchitis virus intracellular rna species to the genome. | avian infectious bronchitis virus, a coronavirus, directed the synthesis of six major single-stranded polyadenylated rna species in infected chicken embryo kidney cells. these rnas include the intracellular form of the genome (rna f) and five smaller rna species (rnas a, b, c, d, and e). species a, b, c, and d are subgenomic rnas and together with the genome form a nested sequence set, with the sequences of each rna contained within every larger rna species (d. f. stern and s. i. t. kennedy, j. ... | 1980 | 6253669 |
| influence of residual moisture and sealing atmosphere on viability of two freeze-dried viral vaccines. | this study demonstrated the complexity of the factors leading to changes in the infectivity titers of freeze-dried canine distemper and poultry infectious bronchitis viral vaccines. the change in moisture content during the storage period was an additional parameter which may influence the infectivity titer. the results emphasized the difficulty of predetermining variations in infectivity titers from the initial residual moisture. the analysis of the variations in infectivity titers during the s ... | 1980 | 6252243 |
| cell mediated and humoral immune response in chickens infected with avian infectious bronchitis. | 1980 | 6250665 | |
| [protective medium for the lyophilization of the infectious bronchitis virus of hens]. | 1980 | 6249020 | |
| coronavirus multiplication strategy. i. identification and characterization of virus-specified rna. | we examined the synthesis of intracellular rna in primary chicken embryo kidney cells infected with the avian coronavirus infectious bronchitis virus. infected cells were labeled with (32)p(i) in the presence of actinomycin d for the duration of the viral multiplication cycle, and nucleic acids were extracted, denatured, and analyzed on agarose slab gels. six major rna species were found. none of these rnas was found in extracts of mock-infected cells. all six of the virus-specified rnas (design ... | 1980 | 6247505 |
| the polypeptide composition of isolated surface projections of avian infectious bronchitis virus. | disruption of avian infectious bronchitis virus (ibv) particles with 4% triton x-100 and 1.0 m-kcl and centrifugation through a sucrose gradient containing 0.1% triton x-100 and 1.0 m-kcl enabled separation of the petal-shaped surface projections. by negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. sds-page of ... | 1980 | 6247435 |
| the polypeptides of infectious bronchitis virus (ibv-41 strain). | the massachusetts strain of avian infectious bronchitis virus was purified from embryonated hens' eggs. four major species of apparent mol. wt. 90 000, 52 000, 29 000 and 26 000 were resolved by sds-polyacrylamide gel electrophoresis. omission of reducing agent failed to resolve the 29 000 mol. wt. component. labelling of acrylamide gels with 125i-concanavalin a indicated that polypeptides of mol. wt. 90 000, 29 000 and 26 000 were glycosylated and, in the absence of reducing agent, that the 29 ... | 1980 | 6247427 |
| protection of laying hens against infectious bronchitis with inactivated emulsion vaccines. | commercially-reared laying chickens were challenged at 31 weeks of age with a virulent infectious bronchitis (ib) virus. they showed a sharp drop in egg production, despite having been vaccinated at four and eight weeks old with live attenuated ib vaccines to a recommended schedule. in contrast, similar birds that had been further immunised at point-of-lay with inactivated oil emulsion ib vaccine, or with a combined ib/newcastle disease (nd) emulsion vaccine, showed no detectable fall in egg pro ... | 1980 | 6246669 |
| two particle types of avian infectious bronchitis virus. | two distinct types of avian infectious bronchitis virus (ibv) particles were isolated on sucrose density gradients. the higher density particles banded at 1.18 g/ml, had typical coronavirus morphology and contained all the structural polypeptides and a complete genome. the less dense particles of density 1.13 g/ml appeared to have typical coronavirus morphology, although they were much more flattened than the more dense particles. furthermore, these particles lacked the ribonucleoprotein polypep ... | 1980 | 6245175 |
| avian infectious bronchitis virus structural polypeptides: effect of different conditions of disruption and comparison of different strains and isolates. | variations in the conditions used for disruption of purified virus involving differences in heat treatment and reducing agent concentration produced little affect on the polypeptide profiles of the massachusetts 41 (m 41) strain of avian infectious bronchitis virus obtained by polyacrylamide gel electrophoresis. comparisons of the structural polypeptides of 12 ibv isolates, consiting of m 41, six serologically related viruses and representatives of five other serotypes, showed that the viruses c ... | 1980 | 6243925 |
| egg production in relation to the results of a long term serological survey of 73 flocks of fowl. | seventy-three flocks of fowl were tested at regular intervals for the presence of precipitins to fowl adenovirus (av) and infectious bronchitis virus (ibv), haemaggluinating inhibiting antibodies to bc in 14 virus, and of agglutinins to mycoplasma gallisepticum (m.g.) and mycoplasma synoviae (m.s.). in all the eight flocks affected with egg drop syndrome (eds '76), egg production problems were associated with increasing numbers of bc14 virus reactors and av reactors. in flocks showing production ... | 1980 | 6243424 |
| rapid serotyping of infectious bronchitis virus isolates with the hemagglutination-inhibition test. | the hemagglutination-inhibition (hi) test was evaluated as a method of typing recent suspect infectious bronchitis virus (ibv) isolates. hemagglutination (ha) antigen was prepared from each isolate by phospholipase c treatment of virus concentrated from allanto-amniotic fluids of infected chicken embryos. an hi test was run with the ha antigen of each isolate against a battery of 17 antisera that had been prepared against different ibv strains classified by virus neutralization (vn). the hi test ... | 1984 | 6207809 |
| muscle protein synthesis and growth of two strains of chicks vaccinated for newcastle disease and infectious bronchitis. | rates of in vitro protein synthesis were monitored during an 8-week period in male broilers and leghorns. the birds received vaccinations during weeks 2 and 5 posthatching for newcastle disease and infectious bronchitis by the administration of a standard dose of the mixed vaccine in the drinking water. both types of birds exhibited a marked decline in the rate of protein synthesis during each week after vaccination. the decline in ribonucleic acid (rna) and deoxyribonucleic acid (dna) levels we ... | 1984 | 6207519 |
| immunomodulatory activity of isoprinosine on experimental viral infections in avian models. | the immunomodulatory activity of isoprinosine treatments have been experimentally verified on chicken infected by three different viruses: newcastle disease, fowl plague and avian infectious bronchitis. in protection tests, positive variations in the mean day of death rather than in the mortality rate were found depending on the modality of treatment. a stimulatory influence on primary anti-newcastle disease virus antibody response was observed. in the avian model the isoprinosine antiviral effe ... | 1984 | 6207123 |
| determination of the antigenic relationships within the massachusetts (m41) type of infectious bronchitis virus using the hemagglutination-inhibition test. | the antigenic relationships, antigenic spectrum, and immunogenicity of seven ibv-massachusetts-41 isolates were investigated using the hemagglutination-inhibition (hi) test. hi titers equal to 32 are considered suspicious, titers lower than 32 are considered negative, and titers higher than 32 are considered positive immune responses to infectious bronchitis virus (ibv). some isolates of massachusetts-41 ( m41 ) were capable of inducing large quantities of antibodies in chickens following inocul ... | 1984 | 6204632 |
| serological comparison and antigenic relationships of seven serotypes of infectious bronchitis virus using the hemagglutination-inhibition test. | the antigenic relationships, antigenic spectrum, and immunogenicity of seven isolates of infectious bronchitis virus (ibv) were examined using the hemagglutination-inhibition (hi) test. because there was a discontinuity of antigenic relationships and a high degree of cross-reactivity among serotypes of ibv in cross-hemagglutination-inhibition tests, the range of antigenic spectrum used to group the serotypes with the hi test should be wider than the limits suggested by the plaque-reduction test. ... | 1984 | 6204631 |
| use of monoclonal antibodies to assess antigenic relationships of avian infectious bronchitis virus serotypes in the united states. | 1984 | 6204515 | |
| survey of respiratory diseases among chicken flocks in japan for avian infectious bronchitis by the neutralization test with embryo-adapted beaudette strain. | 1983 | 6200635 | |
| [comparative serological studies of south german field isolates of hen infectious bronchitis virus and dutch variant strains d207, d3128 and d3896 in tracheal cultures]. | 1983 | 6196022 | |
| evaluation of the hemagglutination-inhibition test for measuring the response of chickens to avian infectious bronchitis virus vaccination. | an infectious bronchitis virus (ibv) hemagglutination-inhibition (hi) test was used to assay serum-antibody titers after ibv vaccination of ibv-susceptible specific-pathogen-free broilers and commercial layers. three-week-old broilers were vaccinated via eye-drop with ibv strains that represent the antigenic spectrum of commercial vaccines--holland, massachusetts 41 (41 ms), connecticut 46, florida 18288, or jmk strain--and revaccinated 3 weeks later with either the same or a heterologous strain ... | 1983 | 6189476 |
| serotypes of avian infectious bronchitis virus isolates from field cases in japan. | eight etiologic agents isolated from field cases in japan were identified as isolates of infectious bronchitis virus by agar-gel diffusion, buoyant-density determination on sucrose-density centrifugation, and morphological study by electron microscope of the purified viruses. in studies of the antigenic relationships of the eight isolates and six known infectious bronchitis viruses, antigenic diversity of these viruses was recognized from the degrees of relatedness using a plaque reduction in th ... | 1982 | 6186239 |
| interferon induction by avian infectious bronchitis virus in chickens. | 1982 | 6182331 | |
| antigenic variation of avian infectious bronchitis virus during replication in bhk-21 cells. | 1982 | 6181761 | |
| mouse hepatitis virus a59: mrna structure and genetic localization of the sequence divergence from hepatotropic strain mhv-3. | the composition and structure of the mouse hepatitis virus (mhv)-specific rna in actinomycin d-treated, infected l-2 cells were studied. seven virus-specific rna species with molecular weights of 0.6 x 10(6), 0.9 x 10(6), 1.2 x 10(6), 1.5 x 10(6), 3.0 x 10(6), 4.0 x 10(6), and 5.4 x 10(6) (equivalent to the viral genome) were detected. t1 oligonucleotide fingerprinting studies suggested that the sequences of each rna species were totally included within the next large rna species. the oligonucle ... | 1981 | 6169842 |
| an immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, cv 777, and several coronaviruses. | a possible antigenic relationship between the porcine enteropathogenic coronavirus-like agent (cvla) and 6 known coronaviruses was examined by immunoelectron microscopy (iem) and by immunofluorescence (if). cvla did not show cross reactivity with infectious bronchitis virus, transmissible gastroenteritis virus (tgev), canine coronavirus (ccv) hemagglutinating encephalomyelitis virus (hev), neonatal calf diarrhea coronavirus (ncdcv) or feline infectious peritonitis virus (fipv). antigenic relatio ... | 1981 | 6166280 |
| antigen requirements and specificity of a microplate enzyme-linked immunosorbent assay (elisa) for detecting infectious bronchitis viral antibodies in chicken serum. | the conditions of a rapid, indirect-enzyme linked immunosorbent assay for infectious bronchitis virus (ibv) antibodies have been established. optimal sensitivity was obtained using 10 micrograms/ml protein concentration of the mass 41 strain purified from infected allantoic fluid. specificity was demonstrated with newcastle disease virus (ndv) antigen-antibody system. negligible crossreactions were observed. after bromelain or lipase treatment ibv had an elisa reactivity similar to untreated par ... | 1981 | 6165342 |
| the coronavirus avian infectious bronchitis virus requires the cell nucleus and host transcriptional factors. | 1980 | 6158788 | |
| effect of different passage histories of infectious bronchitis virus on the sensitivity to inhibitors in chick serum and their removal by trypsin. | sensitivity of the beaudette strain of infectious bronchitis virus (ibv) to non-antibody inhibitors in neutralization tests depended on the passage history of the virus. the chick embryo kidney cell-adapted (e71 cek11) virus was the most sensitive, but after one chick embryo (ce) passage (e71 cek11 e1), this virus showed reversion to the sensitivity of the parent virus (e71). ibv inhibitors in chicken serum could be removed by treatment with trypsin but not with phospholipase c. | 1984 | 6147998 |
| demonstration of 7-nm projections on human and avian coronaviruses. | direct electron microscopy (em) of human coronavirus (hcv) strain oc43 and avian infectious bronchitis virus (ibv) strain f revealed particles with small granular projections about 7 nm in size in addition to the characteristic coronavirus particles with projections of 20 nm. relationship of the short fringed form to the conventional coronavirus particle is established by ordinary immune electron microscopy (iem) and immunosorbent electron microscopy (isem). | 1984 | 6099927 |
| sequence of the membrane protein gene from avian coronavirus ibv. | cdna clones prepared from genomic rna of coronavirus ibv have been sequenced. the nucleotide sequence for the complete 5' region of mrna c, which is not present in mrnas a and b, has been determined. a sequence of 1224 bases is presented which contains a long open reading frame predicting a polypeptide of molecular weight 25 443. this is in agreement with the molecular weight of 23 000 reported for the unglycosylated form of the membrane polypeptide. | 1984 | 6099661 |
| replication and morphogenesis of avian coronavirus in vero cells and their inhibition by monensin. | avian infectious bronchitis virus (ibv) was adapted to vero cells by serial passage. no significant inhibition of ibv replication was observed when infected vero cells were treated with alpha-amanitin or actinomycin d. in thin sections of infected cells, assembly of ibv was observed at the rough endoplasmic reticulum (rer), and mature ibv particles were located in dilated cisternae of the rer as well as in smooth cytoplasmic vesicles. in addition to typical ibv particles, enveloped particles con ... | 1984 | 6099655 |
| nephropathogenicity of infectious bronchitis virus. | four strains of infectious bronchitis virus (ibv) were compared in chickens as to nephropathogenicity. two of the viruses were from the united states, one was from australia, and one from italy. each has been described as nephropathogenic. the australian strain proved to be most pathogenic and was then followed by the italian strain and, finally, the two viruses from the united states. with an increase in the age of the chickens there was an apparent decrease in pathogenic effect. all of the vir ... | 1984 | 6099569 |
| use of egg yolk in serological tests (elisa and hi) to detect antibody to newcastle disease, infectious bronchitis, and mycoplasma gallisepticum. | serum and yolks from commercial flocks and from hens exposed to newcastle disease virus (ndv), infectious bronchitis virus (ibv), and mycoplasma gallisepticum (mg) were tested for immunoglobulin g antibody by the enzyme-linked immunosorbent assay (elisa) and the hemagglutination-inhibition (hi) test. yolks prepared by chloroform extraction and low-speed centrifugation performed well in the serological tests used and were a suitable alternative to serum for antibody determination by the elisa for ... | 1984 | 6098252 |
| monoclonal antibodies to the s1 spike and membrane proteins of avian infectious bronchitis coronavirus strain massachusetts m41. | we have established four murine hybridoma cell lines which secrete specific antibody to avian infectious bronchitis virus (ibv) strain massachusetts m41. two monoclonal antibodies reacted with the spike protein and two reacted with the membrane protein. the specificity of the monoclonal antibodies for the external structural proteins was detected by immunoprecipitations using radiolabelled virus. the reactions of the monoclonal antibodies showed that one (s1) of the two glycopolypeptides associa ... | 1984 | 6096499 |
| influence of newcastle disease and infectious bronchitis live virus vaccines on immune response against infectious laryngotracheitis live virus vaccine in chickens. | 1984 | 6092761 | |
| sequencing of coronavirus ibv genomic rna: a 195-base open reading frame encoded by mrna b. | dna sequencing of genomic cdna clones of avian infectious bronchitis virus (ibv) has been carried out. 770 bases have been determined which include genomic sequences spanning the 5' termini of the two smallest mrnas of the 3'-coterminal "nested" set: mrna a and mrna b. this region contains the complete coding sequences for mrna b which are additional to those present in mrna a. two open reading frames are present, predicting proteins of mrs 7500 and 9500. | 1984 | 6092234 |
| in vitro and in vivo studies with an avian reovirus derived from a temperature-sensitive mutant clone. | a temperature-sensitive (ts) mutant of avian reovirus isolate maine 1-203 was obtained at 41 c. the 19th passage of this mutant (ts 19) was utilized for in vitro studies comparing its replication in macrophage cultures with that of its parent strain and an apathogenic s1133 virus. in vivo studies were initiated to determine the ts mutant's pathogenicity for day-old chicks, safety and protective levels for 6-day-old chicks, and interference with marek's disease, newcastle, and infectious bronchit ... | 1984 | 6091605 |
| occurrence and significance of infectious bronchitis virus variant strains in egg and broiler production in the netherlands. | despite vaccination against infectious bronchitis virus (ibv) with the massachusetts type vaccine viruses h120 and h52 in the netherlands, an increasing number of properly vaccinated flocks have suffered from the disease since 1978. in the years 1978-1982, the virus was isolated from 162 ibv suspected flocks. cross-virus-neutralization tests showed that the majority (67 per cent) of these isolates belonged to serotypes other than the massachusetts type, the connecticut-, florida-, iowa 97-, iowa ... | 1984 | 6091319 |
| sequential development of humoral immunity and assessment of protection in chickens following vaccination and challenge with avian infectious bronchitis virus. | chicks without specific maternal antibody to avian infectious bronchitis virus were vaccinated intranasally at two weeks old with a live h120 vaccine and a proportion revaccinated with a live h52 vaccine at 15 weeks. groups of these birds were then challenged intratracheally with the massachusetts m41 strain at varying intervals and their humoral antibody responses monitored by neutralisation and haemagglutination-inhibition tests. protection was assessed by the presence of tracheal ciliostasis ... | 1984 | 6089286 |
| a leader sequence is present on mrna a of avian infectious bronchitis virus. | the subgenomic mrnas of the fowl coronavirus infectious bronchitis virus (ibv) form a 3' co-terminal or 'nested' set. the rnase t1 oligonucleotide mapping data which demonstrated this form of sequence organization provided no evidence for the presence of non-contiguous (leader) sequences fused to the 5' termini of the mrnas. however, we have been able to demonstrate, by extension of an end-labelled synthetic oligonucleotide primer, the presence of a leader sequence on ibv mrna a. sequencing by t ... | 1984 | 6086827 |
| electron microscopy of infectious bronchitis virus. | 1967 | 6070456 | |
| immunity following aerosol exposure to high-embryo-passage avian infectious bronchitis virus. | 1967 | 6069470 | |
| avian infectious bronchitis. | 1967 | 6068045 | |
| adaptation of the massachusetts strain of infectious bronchitis virus to turkey embryos. | 1967 | 6067604 | |
| [research on the sensitivity of various small birds to avian infectious bronchitis virus]. | 1966 | 6012003 | |
| titration of infectious bronchitis virus in tracheas of susceptible and immune chickens. | 1969 | 5818062 | |
| [differentiation of field strains of avian bronchitis virus (ibv)]. | 1969 | 5817689 | |
| serologic and immunologic properties of a recent isolate of infectious bronchitis virus. | 1969 | 5816048 | |
| [comparative studies on the pathogenicity, histo-pathogenesis and immunizing ability of two attenuated strains of avian infectious bronchitis virus]. | 1969 | 5815857 | |
| the effect of unilateral ureteral ligation on mortality in chickens experimentally infected with nephrosis-inducing infectious bronchitis virus. | 1969 | 5815412 | |
| the control of avian infectious bronchitis/nephrosis in australia. | 1969 | 5814107 | |
| studies on avian infectious bronchitis virus. 2. incidence of the virus in broiler and layer flocks, by isolation and serological methods. | 1969 | 5806096 | |
| studies on avian infectious bronchitis virus. i. distribution and survival of the virus in tissues of affected chickens and studies on the carrier status. | 1969 | 5806095 | |
| neutralizing antibody complex of infectious bronchitis virus. | 1969 | 5773320 | |
| the pathogenicity of an infectious bronchitis virus for laying hens, with observations on pathogenesis. | 1968 | 5750632 | |
| neutralization of infectious bronchitis virus by human sera. | 1968 | 5748864 | |
| replication and cytopathogenicity of avian infectious bronchitis virus in chicken embryo kidney cells. | 1968 | 5729633 | |
| an interference type of serological test for infectious bronchitis virus using newcastle disease virus. | 1968 | 5699787 | |
| the morphological and biological effects of various antisera on avian infectious bronchitis virus. | 1968 | 5692879 | |
| morphogenesis of avian infectious bronchitis virus and a related human virus (strain 229e). | avian infectious bronchitis virus (ibv) and strain 229e, a virus recently recovered from patients with colds, have been shown to possess a similar distinctive morphology in negatively stained preparations. an electron microscopic study of the morphogenesis of ibv in the chorioallantoic membrane and of strain 229e in wi-38 cells was performed. in infected cells, round electron-dense particles 82 mmu in diameter were observed to form by a process of budding from membranes of the endoplasmic reticu ... | 1967 | 5630226 |
| [serological study of the spread in italy of different types of the virus of avian infectious bronchitis]. | 1967 | 5596592 | |
| [infectious bronchitis of chickens. iv. characterization of new field strain of ib virus (ibv-10)]. | 1967 | 5594731 | |
| interaction of mycoplasma gallisepticum, mild strains of newcastle disease virus and infectious bronchitis virus in chickens. | 1971 | 5569701 | |
| abnormalities in laying chickens following exposure to infectious bronchitis virus at one day old. | 1971 | 5547755 | |
| pathogenesis of oviduct lesions in immature chickens following exposure to infectious bronchitis virus at one day old. | 1971 | 5547754 |