Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| coronary vein infusion of multipotent stromal cells from bone marrow preserves cardiac function in swine ischemic cardiomyopathy via enhanced neovascularization. | few reports have examined the effects of adult bone marrow multipotent stromal cells (mscs) on large animals, and no useful method has been established for msc implantation. in this study, we investigate the effects of msc infusion from the coronary vein in a swine model of chronic myocardial infarction (mi). mi was induced in domestic swine by placing beads in the left coronary artery. bone marrow cells were aspirated and then cultured to isolate the mscs. at 4 weeks after mi, mscs labeled with ... | 2011 | 21283079 |
| molecular mechanism by which amp-activated protein kinase activation promotes glycogen accumulation in muscle. | during energy stress, amp-activated protein kinase (ampk) promotes glucose transport and glycolysis for atp production, while it is thought to inhibit anabolic glycogen synthesis by suppressing the activity of glycogen synthase (gs) to maintain the energy balance in muscle. paradoxically, chronic activation of ampk causes an increase in glycogen accumulation in skeletal and cardiac muscles, which in some cases is associated with cardiac dysfunction. the aim of this study was to elucidate the mol ... | 2011 | 21282366 |
| identification of low and high frequency ranges for heart rate variability and blood pressure variability analyses using pharmacological autonomic blockade with atropine and propranolol in swine. | understanding autonomic nervous system functioning, which mediates behavioral and physiological responses to stress, offers great potential for assessing farm animal stress and welfare. evaluation of heart rate variability (hrv) and blood pressure variability (bpv), using time and frequency domain analyses may provide a sensitive and reliable measure of affective states and stress-mediated changes in sympathetic and parasympathetic tones. the aim of this research was to define low (lf) and high ... | 2011 | 21281655 |
| application of gis in epizootiological surveillance of swine trichinellosis in one endemic district in serbia. | application of new tools for epizootiological investigations in veterinary medicine, such as geographical information systems (gis), offers a new approach and possibilities for the eradication or control of infectious diseases. gis is particularly useful for research conducted in small areas strongly impacted by man. trichinellosis is a world-wide zoonosis, which is endemic in some european countries, balkan district and serbia in particular. there are very few data on gis application in the fie ... | 2010 | 21275245 |
| differential mrna expression of genes in the porcine adrenal gland associated with psychosocial stress. | to gain insight into the adrenal stress response, we analysed differential mrna expression of genes associated with psychosocial stress in the pig (sus scrofa domestica). various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. we selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. to identify differentially expressed genes, we ... | 2011 | 21266515 |
| shen-fu injection attenuates postresuscitation myocardial dysfunction in a porcine model of cardiac arrest. | to investigate the effect of shen-fu injection (sfi) for the management of postresuscitation myocardial dysfunction in a porcine model of cardiac arrest. ventricular fibrillation was induced electrically in anesthetized domestic swine. after 4 min of untreated ventricular fibrillation, cardiopulmonary resuscitation was initiated. sixteen successfully resuscitated pigs were randomized to receive a continuous infusion of either sfi (0.24 mg/min) or saline placebo for 6 h, beginning 15 min after re ... | 2011 | 21263380 |
| distinctive differences in long terminal repeat sequences between γ1 endogenous retroviruses of african and eurasian suid species. | diversity of long terminal repeats (ltrs) from γ1 endogenous retroviruses (ervs) was analysed by dna sequencing in 10 species of the family suidae (suids, pigs and hogs). phylogenetic analysis separated ltr sequences into two groups which correlated approximately with either the previously described cluster i and iii, or the clusters ii, iv and v. interestingly, a specific ltr exhibiting a novel molecular rearrangement was identified exclusively within african host species when compared to ltrs ... | 2011 | 21256982 |
| microrna expression profiling of the porcine developing brain. | micrornas are small, non-coding rna molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. in particular, the developing brain contains an impressive diversity of micrornas. most microrna expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. the domestic pig is considered to be an excellent, alternate, large ... | 2011 | 21253018 |
| identification of a second gene associated with variation in vertebral number in domestic pigs. | the number of vertebrae in pigs varies and is associated with body size. wild boars have 19 vertebrae, but european commercial breeds for pork production have 20 to 23 vertebrae. we previously identified two quantitative trait loci (qtls) for number of vertebrae on sus scrofa chromosomes (ssc) 1 and 7, and reported that an orphan nuclear receptor, nr6a1, was located at the qtl on ssc1. at the nr6a1 locus, wild boars and asian local breed pigs had the wild-type allele and european commercial-bree ... | 2011 | 21232157 |
| suture-free anastomosis of the colon. experimental comparison of two cyanoacrylate adhesives. | we explored the potential of two cyanoacrylate tissue adhesives for constructing colonic anastomoses. | 2011 | 21229329 |
| cloning, characterization and diagnostic performance of the salivary lipocalin protein tsgp1 from ornithodoros moubata. | the argasid tick ornithodoros moubata is distributed throughout south and east africa and madagascar, where it colonizes wild and domestic habitats and feeds on warthogs, domestic swine, and humans. this argasid transmits the spirochete borrelia duttonii, causing east african tick-borne relapsing fever in humans, and the african swine fever virus, which causes a highly lethal haemorrhagic disease in pigs. tick surveillance and the elimination of o. moubata from synanthropic environments (human d ... | 2010 | 21216104 |
| genetic characterization of african swine fever viruses from a 2008 outbreak in tanzania. | outbreaks of african swine fever (asf) have been reported in the past from several countries in sub-saharan africa. the aim of this study was to genotype asf viruses (asfvs) from the 2008 outbreak in morogoro and dar es salaam regions of tanzania. tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with pcr and genome sequencing methods using asfv-specific primer sets. nucleotide sequence data were obtained for the b646l (p72), e183l ... | 2010 | 21214868 |
| comparison of electrocardiographic recordings in open-chest and closed-chest swine models. | the aim of our study was to compare the electrocardiographic recordings in an experimental open-chest swine model before and after left-sided thoracotomy to detect any surgery-induced fluctuations that might interfere with subsequent experimental interventions. we obtained electrocardiograms from 8 deeply anesthetized domestic swine and compared the respective st-segment potentials obtained after vascular surgery and after left-sided thoracotomy and dissection of the left anterior descending cor ... | 2010 | 21205452 |
| infrared skin damage thresholds from 1940-nm continuous-wave laser exposures. | a series of experiments are conducted in vivo using yucatan mini-pigs (sus scrofa domestica) to determine thermal damage thresholds to the skin from 1940-nm continuous-wave thulium fiber laser irradiation. experiments employ exposure durations from 10 ms to 10 s and beam diameters of approximately 4.8 to 18 mm. thermal imagery data provide a time-dependent surface temperature response from the laser. a damage endpoint of minimally visible effect is employed to determine threshold for damage at 1 ... | 2010 | 21198172 |
| scanning electron microscopy analysis of luminal inflammation induced by different types of coronary stent in an animal model. | there is histological evidence that drug-eluting stents are associated with delayed endothelialization and a persistent inflammatory state. moreover, clusters of inflammatory cells have been observed on luminal surfaces by scanning electron microscopy. with the aim of quantifying this inflammatory response, we implanted one bare-metal stent and two drug-eluting stents containing different doses of vinblastine embedded in the same polymer into the coronary arteries of 12 domestic pigs. the densit ... | 2010 | 21195533 |
| single-port laparoscopic cholecystectomy with the x-cone: a feasibility study in 9 pigs. | virtually any port system for single-port laparoscopic surgery is for single usage only. the aim of this study was to trial the novel and completely reusable port x-cone in order to perform a cholecystectomy by one infraumbilical incision. | 2010 | 21193478 |
| irreversible electroporation in a swine lung model. | this study was designed to evaluate the safety and tissue effects of ire in a swine lung model. | 2010 | 21191587 |
| prevalence and genotypes of toxoplasma gondii in feline faeces (oocysts) and meat from sheep, cattle and pigs in switzerland. | the protozoan parasite toxoplasma gondii infects almost all warm blooded animal species including humans, and is one of the most prevalent zoonotic parasites worldwide. post-natal infection in humans is acquired through oral uptake of sporulated t. gondii oocysts or by ingestion of parasite tissue cysts upon consumption of raw or undercooked meat. this study was undertaken to determine the prevalence of oocyst-shedding by cats and to assess the level of infection with t. gondii in meat-producing ... | 2010 | 21183278 |
| effects of an alpha-4 integrin inhibitor on restenosis in a new porcine model combining endothelial denudation and stent placement. | restenosis remains the main complication of balloon angioplasty and/or stent implantation. preclinical testing of new pharmacologic agents preventing restenosis largely rely on porcine models, where restenosis is assessed after endothelial abrasion of the arterial wall or stent implantation. we combined endothelial cell denudation and implantation of stents to develop a new clinically relevant porcine model of restenosis, and used this model to determine the effects of an α4 integrin inhibitor, ... | 2010 | 21179441 |
| effects of human perturbation on the genetic make-up of an island population: the case of the sardinian wild boar. | game species are often manipulated by human beings, whose activities can deeply affect their genetic make-up and population structure. we focused on a geographically isolated wild boar population (sardinia, italy), which is classified, together with the corsican population, as a separate subspecies (sus scrofa meridionalis). two hundred and ten wild boars collected across sardinia were analysed with a set of 10 microsatellites and compared with 296 reference genotypes from continental wild popul ... | 2010 | 21179064 |
| experimental infection of common warthogs (phacochoerus africanus) and bushpigs (potamochoerus larvatus) with classical swine fever virus ii: a comparative histopathological study. | wild african suidae, the common warthog (phacochoerus africanus) and bushpig (potamochoerus larvatus), were experimentally infected with classical swine fever (csf) virus following the diagnosis of csf subtype 2.1 in domestic pigs in south africa in 2005. no data regarding the susceptibility or potential lesions of these african wild suids are available. seven subadult warthogs and six bushpigs were captured and infected intranasally with the south african isolate. two in-contact control animals ... | 2010 | 21176120 |
| prins detection of 18s rdna in pig, red fox and chinese raccoon dog, and centromere dna in horse. | the fluorescence in situ hybridization (fish) technique is widely used in animal cytogenetics. contrary to fish procedure, primed in situ dna synthesis (prins) does not require the dna probe preparation (design, synthesis, gel purification of pcr products and labeling). the prins method with primers used as 'dna probes' is both pcr-sensitive and allows for chromosomal localization of dna sequences. here, we show the application of prins reaction with one unlabeled oligonucleotide pair to identif ... | 2010 | 21166802 |
| comparison of purified olive oil and silver sulfadiazine in the treatment of partial thickness porcine burns. | introduction: burns are widespread in the developed world, and expensive burn dressings are not universally available. most burn patients suffer from a partial thickness burn that can be treated conservatively. nevertheless, the ideal dressing for the burn wound has not been identified. we performed an animal experiment to compare the healing of partial thickness burns treated with silver sulfadiazine (ssd) and olive oil. methods: a randomized controlled animal experiment was conducted on 3 anes ... | 2010 | 21159471 |
| characterization of plasma triiodophenol binding proteins in vertebrates and tissue distribution of triiodophenol in rana catesbeiana tadpoles. | we investigated the interaction of 2,4,6-triiodophenol (tip), a potent thyroid hormone disrupting chemical, with serum proteins from rainbow trout (onchorhynchus mykiss), bullfrog (rana catesbeiana), chicken (gallus gallus), pig (sus scrofa domesticus), and rat (rattus norvegicus) using a [(125)i]tip binding assay, gel filtration chromatography, and native polyacrylamide gel electrophoresis. [(125)i]tip bound non-specifically to proteins in trout serum, specifically but weakly to proteins in bul ... | 2010 | 21147258 |
| a preclinical in vivo investigation of high-intensity focused ultrasound combined with radiotherapy. | this study aims to perform an in vivo investigation evaluating the injury to the pancreas and adjacent tissue of swine resulting with high-intensity focused ultrasound (hifu) combined with radiotherapy (rt). the protocol was approved by the animal ethics committee at the peking university first hospital. a total of 12 domestic swine were divided into four groups: control, hifu only, rt only and hifu + rt. the injury to the pancreas, adjacent tissue and tissue within the acoustic path of the hifu ... | 2011 | 21144959 |
| feasibility and safety of endoscopic transesophageal access and closure using a maryland dissector and a self-expanding metal stent. | background: secure access and reliable closure is paramount in the setting of transesophageal mediastinal endoscopic surgery. the purpose of this study was to develop a secure transesophageal access technique and to evaluate the feasibility, safety, and efficacy of a novel covered, self-expanding, retractable stent for closure of 15-mm esophageal defects. methods: fifteen-millimeter esophagotomies were created in 18 domestic pigs using needle knife puncture and balloon dilatation or a blunt diss ... | 2010 | 21136086 |
| oct4-enhanced green fluorescent protein transgenic pigs: a new large animal model for reprogramming studies. | the domesticated pig has emerged as an important tool for development of surgical techniques, advancement of xenotransplantation, creation of important disease models, and preclinical testing of novel cell therapies. however, germ line-competent pluripotent porcine stem cells have not yet been derived. this has been a major obstacle to genetic modification of pigs. the transcription factor oct4 is essential for the maintenance of pluripotency and for reprogramming somatic cells to a pluripotent ... | 2011 | 21126163 |
| first detection of african swine fever virus in ornithodoros porcinus in madagascar and new insights into tick distribution and taxonomy. | abstract: | 2010 | 21118485 |
| vertebral column, rib and sternal muscles of sulawesi babirusa (babyrousa celebensis). | the muscular anatomy of the vertebrae, ribs and sternebrae of the sulawesi babirusa (babyrousa celebensis) is described. there are many similarities to the anatomy of the domestic pig (sus scrofa). however, unlike other pigs, the m. spinalis et semispinalis cervicis et thoracis has an origin from the twelfth thoracic mamillary process. similarly, the babyrousa does not have the lumbar part of the m. obliquus internus abdominis which is found in other pigs. the m. sacrocaudalis dorsalis medialis ... | 2010 | 21108656 |
| distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays. | double fertilization in flowering plants refers to a process in which two sperm cells, carried by the pollen tube, fertilize both the egg and the central cell after their release into a synergid cell of the female gametophyte. the molecular processes by which the female gametophytic cells express their unique functions during fertilization are not well understood. genes expressed in egg and synergid cells might be important for multiple stages of the plant reproductive process. here, we profiled ... | 2010 | 21106719 |
| an on-farm survey of spatial and temporal stratifications of 17β-estradiol concentrations. | 17β-estradiol (e2) is a natural estrogenic hormone found in animal manure and urine, which may cause endocrine disruption in sensitive organisms. 17β-estradiol has been widely detected in the environment, and animal agriculture may be an important source. the objectives of this study were to investigate the potential sources and/or spatial and temporal characteristics contributing to detections of e2 at the farm-scale. soil cores, segmented into 0.15m increments, were taken down to the water tab ... | 2010 | 21094971 |
| gene expression of serotonin and dopamine receptors and monoamine oxidase-a in the brain of dominant and subordinate pubertal domestic pigs (sus scrofa) fed a β-adrenoreceptor agonist. | aggression is a major source of social stress with negative effects on health and well-being, yet limited information is known about the molecular mechanisms mediating aggressive behavior in swine. ractopamine (rac) is a β-adrenoreceptor agonist that enhances growth but increases aggressive behaviors in female pigs. thus, the effects of rac, sex, and social rank on the mrna abundance of genes encoding serotonin and dopamine receptors, and monoamine oxidase (mao)-a in brains of sub-adult pigs wer ... | 2010 | 21094150 |
| mechanical chest compressions with trapezoidal waveform improve haemodynamics during cardiac arrest. | during manual chest compressions for cardiac arrest the waveforms of chest compressions are generally sinusoidal, whereas mechanical chest compression devices can have different waveforms, including trapezoidal. we studied the haemodynamic differences of such waveforms in a porcine model of cardiac arrest. | 2010 | 21093141 |
| prevalence of porcine circoviruses in transylvanian wild boars, detected by real-time pcr--short communication. | porcine circoviruses (pcv) are widespread in domestic pigs worldwide and there is growing information about the presence of pcv in other suid species. based on serological studies with sera of wild boars, it was established that pcv1 was present in these animals and antibodies specific to pcv2 were also detected in wild boars living in captivity or in sylvatic areas, both with or without clinical signs of pmws. studies including pcv2 genome or antigen detection confirmed the previous findings. t ... | 2010 | 21087917 |
| genetic variations and sequences analysis of mtatp6 and mtatp8 genes among different chinese pig breeds. | to investigate the genetic variations of mtatp6 and mtatp8 genes among different chinese pig breeds, two fragments of 425 and 743 bp containing the whole coding region of mtatp8 and mtatp6 genes were amplified with 805 individuals from 23 chinese local pig breeds, three types of chinese wild boars and three european pig breeds. sequence comparison identified a total number of 17 substitutions including six variable sites in mtatp8 and eleven substitutions in mtatp6 gene. the restriction enzyme f ... | 2010 | 21077971 |
| noninvasive mr characterization of structural and functional components of reperfused infarct. | left ventricular (lv) remodeling is a highly complex phenomenon that starts soon after infarction and progresses to extensive regional and global architectural changes over time. | 2010 | 21062130 |
| hybrid transureteral natural orifice translumenal endoscopic nephrectomy: a feasibility study in the porcine model. | natural orifice approaches for nephrectomy have included access via the stomach, vagina, bladder, and rectum. the use of the ureter as a natural orifice for natural orifice translumenal endoscopic surgery (notes) nephrectomy has not been previously reported. the purpose of this study is to test the feasibility of transureteral laparoscopic notes nephrectomy. | 2010 | 21058889 |
| nucleotide variability of the porcine serpina6 gene and the origin of a putative causal mutation associated with meat quality. | the serpin peptidase inhibitor, clade a, member 6 gene (serpina6), also known as corticosteroid-binding globulin or cbg, is involved in obesity and stress sensitivity. previous studies have reported putative causal mutations within that gene in the porcine species. to characterize a hypothetical selective footprint, we have resequenced approximately 6 kb of coding and non-coding fragments in 20 pigs comprising domestic breeds and wild boars from asia and europe. nucleotide variability was found ... | 2010 | 21054451 |
| comparative study of the osseointegration of dental implants after different bone augmentation techniques: vascularized femur flap, non-vascularized femur graft and mandibular bone graft. | objectives: the purpose of this study was to evaluate the osseointegration of the dental implants placed into the mandible augmented with different techniques in pigs. material and methods: four adult domestic pigs were used. horizontal augmentation of the mandible was performed in animals by using vascularized femur flap (vff), non-vascularized femur graft (nvfg) and monocortical mandibular block graft (mg). after 5 months of healing 10 dental implants were placed into each augmented site. the ... | 2010 | 21044163 |
| novel robotic da vinci instruments for laparoendoscopic single-site surgery. | to describe novel robotic laparoendoscopic single-site surgery (r-less) instruments, and present the initial laboratory experience in urology. | 2010 | 20980046 |
| virulence genes and plasmid profiles in rhodococcus equi isolates from domestic pigs and wild boars (sus scrofa) in brazil. | the virulence genes and plasmid profiles of 23 rhodococcus equi isolates from 258 lymph nodes from domestic pigs (129 nodes with lesions and 129 without lesions) and 120 lymph nodes from slaughtered wild boars (60 nodes with lesions and 60 without) were characterized. r. equi was obtained from 19 lymph nodes of domestic pigs, 17 with, and two without lesions, and from four lymph nodes with lesions, from wild boars. the 23 isolates were tested for the presence of vapa and vapb genes, responsible ... | 2010 | 20980033 |
| a 6-bp deletion in the tyrp1 gene causes the brown colouration phenotype in chinese indigenous pigs. | brown coat colour has been described in chinese-tibetan, kele, and dahe pigs. here, we report the identification of a causal mutation underlying the brown colouration. we performed a genome-wide association study (gwas) on tibetan and kele pigs, and found that brown colours in chinese breeds are controlled by a single locus on pig chromosome 1. by using a haplotype-sharing analysis, we refined the critical region to a 1.5-mb interval that encompasses only one pigmentation gene: tyrosinase-relate ... | 2010 | 20978532 |
| [changes in myocardial connexin 43 during ventricular fibrillation]. | to observe changes in connexin 43 (cx43) after ventricular fibrillation (vf) and the effects of rotigaptide (zp123) on cx43. | 2010 | 20977841 |
| african swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site. | the role of the ancestral sylvatic cycle of the african swine fever virus (asfv) is not well understood in the endemic areas of eastern africa. we therefore analysed the asf infection status on samples collected from 51 free-ranging warthogs (phacocherus africanus) and 1576 ornithodorus porcinus ticks from 26 independent warthog burrows at a single ranch in kenya. abattoir samples from 83 domestic pigs without clinical symptoms, originating from specific locations with no recent reported asf out ... | 2010 | 20965989 |
| left ventricular pacing lead insertion via the coronary sinus cardioplegia cannula: a novel method for temporary biventricular pacing during reoperative cardiac surgery. | objective: temporary biventricular pacing to treat low output states after cardiac surgery is an active area of investigation. reoperative cases are not studied due to adhesions, which preclude left ventricular mobilization to place epicardial pacing wires. in such patients, inserting a temporary left ventricular lead via the coronary sinus cardioplegia cannula may allow for biventricular pacing. we developed a novel technique for intraoperative left ventricular lead placement. methods: eight do ... | 2010 | 20965517 |
| an elisa based on a truncated soluble orf2 protein for the detection of pcv2 antibodies in domestic pigs. | postweaning multisystemic wasting syndrome (pmws) is an important swine disease that is closely associated with porcine circovirus type 2 (pcv2). the capsid protein (cap protein) is a major structural protein that has at least three immunoreactive regions, and it can be a suitable candidate antigen for detecting the specific antibodies of a pcv2 infection. in the present study, an indirect enzyme-linked immunosorbent assay (tcelisa) based on a truncated soluble cap protein produced in escherichi ... | 2010 | 20960293 |
| ochrobactrum pecoris sp. nov., isolated from farm animals. | two gram-negative, rod-shaped, non-spore-forming strains, 08rb2639(t) and 08rb2781-1 isolated from sheep (ovis aries) and boar (sus scrofa domestica), respectively, were studied for their taxonomic position. by 16s rrna gene sequencing, these strains revealed identical sequences and were shown to belong to the alphaproteobacteria with 97.8 % sequence similarity to ochrobactrum rhizosphaerae pr17(t), ochrobactrum pituitosum ccug 50899(t) , ochrobactrum tritici scii24(t) and ochrobactrum haematoph ... | 2010 | 20952542 |
| magnetically controllable gastrointestinal steering of video capsules. | wireless capsule endoscopy (wce) allows for comfortable video explorations of the gastrointestinal (gi) tract, with special indication for the small bowel. in the other segments of the gi tract also accessible to probe gastroscopy and colonscopy, wce still exhibits poorer diagnostic efficacy. its main drawback is the impossibility of controlling the capsule movement, which is randomly driven by peristalsis and gravity. to solve this problem, magnetic maneuvering has recently become a thrust rese ... | 2010 | 20952324 |
| coronary bare metal stent implantation in homozygous ldl receptor deficient swine induces a neointimal formation pattern similar to humans. | to date, most of all new developments in stent technologies are tested in normal animals. although invaluable in the evaluation of device safety, the juvenile domestic swine (ds) do not follow the biological healing response occurring in humans following coronary stent implantation. by using a novel swine breed afflicted with familial hypercholesterolemia (fhs), we aimed to analyse the vascular response occurring following bare metal stent (bms) implantation by comparing in vivo endovascular ima ... | 2010 | 20950808 |
| next-generation sequencing of the trichinella murrelli mitochondrial genome allows comprehensive comparison of its divergence from the principal agent of human trichinellosis, trichinella spiralis. | the mitochondrial genome's non-recombinant mode of inheritance and relatively rapid rate of evolution has promoted its use as a marker for studying the biogeographic history and evolutionary interrelationships among many metazoan species. a modest portion of the mitochondrial genome has been defined for 12 species and genotypes of parasites in the genus trichinella, but its adequacy in representing the mitochondrial genome as a whole remains unclear, as the complete coding sequence has been char ... | 2010 | 20946970 |
| a biochemical comparison of the in vivo effects of bulbine frutescens and bulbine natalensis on cutaneous wound healing. | in south africa the local population relies extensively on indigenous plants in the formulation of traditional medicines to treat skin ailments. the scientific merits of many of these plants used to treat wounds and burns are yet to be validated. bulbine natalensis and bulbine frutescens of the asphodelaceae family are indigenous to only southern africa and are widely used as a skin remedy. this study aimed to explore the scientific value of these plants through investigating the in vivo biochem ... | 2010 | 20937372 |
| identification of a novel astrovirus in a domestic pig in hungary. | the family astroviridae consists of two genera, avastrovirus and mamastrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. we serendipitously identified a novel porcine astrovirus in a fecal specimen from a domestic pig (sus scrofa domestica) in hungary. sequencing of a fragment indicated that it was an orf1b/orf2/3'utr sequence, and it has been submitted to the database as porcine astrovirus type 2 (pastv-2/hungary/2007) with accession number g ... | 2010 | 20931250 |
| meiotic studies in infertile domestic pig-babirusa hybrids. | mating of a babirusa (babyrousa babyrussa) boar and a domestic sow (sus scrofa) resulted in the birth of 5 live domestic pig-babirusa hybrid piglets. chromosome analysis of one of the surviving males confirmed that they were domestic pig-babirusa hybrids by revealing the presence of a complete haploid set of 19 porcine chromosomes as well as a complete haploid set of 19 babirusa chromosomes in the karyotype. none of the surviving piglets, two males and one female, had shown signs of sexual matur ... | 2010 | 20924163 |
| the effect of nesting material on the nest-building and maternal behavior of domestic sows and piglet production. | nest building is an important part of maternal behavior in domestic pigs. the aim of the study was to assess the effect of nesting material sawdust vs. straw on sow behavior 24 h before and after birth of the first piglet (bfp) and piglet production. sows, housed in farrowing crates, were randomly divided into 2 treatments: sawdust (n = 12) and straw (n = 13). sawdust and straw were provided during the pre- and parturient period; after parturition, straw was given to both experimental groups. th ... | 2010 | 20889685 |
| a novel device for rapid cleaning of poorly prepared colons. | adequate colon preparation is essential for the quality and accuracy of colonoscopy and has a significant influence on related costs. the aim of this study was to assess the efficacy and safety of a novel attachable colon-cleaning device used during colonoscopies in porcine colon. | 2010 | 20886401 |
| confirmation of needle cricothyroidotomy using an esophageal detector device in a porcine model. | the difficult intubation is one of the most trying situations encountered in airway management. transtracheal jet ventilation via needle cricothyroidotomy can provide temporary oxygenation in this critical situation in a minimally invasive fashion. the esophageal detector device (edd) has been previously shown to be effective in determining the tracheal placement of endotracheal tubes. in this experiment, three commonly available edds are utilized to accurately identify 15 of 15 intratracheal pl ... | 2010 | 20882932 |
| distribution of orf2 and orf3 genotypes of porcine circovirus type 2 (pcv-2) in wild boars and domestic pigs in germany. | porcine circovirus 2 (pcv-2), the essential infectious agent in pcvd (porcine circovirus diseases) circulates at high rates among domestic pig and wild boar populations. wild boars may be viremic and shed the virus with excretions and secretions, and thus serve as a reservoir for domestic pig pcv-2 infection. we hypothesize that pcv-2 strains circulating in wild boars and in domestic pigs are significantly different and thus, partially independent. to prove this hypothesis, the present study inv ... | 2010 | 20869816 |
| comparison of the substrate kinetics of pig cyp3a29 with pig liver microsomes and human cyp3a4. | cyp (cytochrome p450) 3a29 in pigs could be an important candidate gene responsible for xenobiotic metabolism, similar to cyp3a4 in humans. accordingly, the tissue expression of cyp3a29 mrna in domestic pigs has been determined by a real-time pcr. the enzymatic properties of cyp3a29, cyp3a4 and plm (pig liver microsomes) were compared by kinetic analysis of tst (testosterone) 6β-hydroxylation and nif (nifedipine) oxidation. cyp3a29 mrna was highly expressed in the liver and small intestines of d ... | 2011 | 20863320 |
| video: transcervical videoscopic esophageal dissection in minimally invasive esophagectomy. | minimally invasive esophagectomy (mie) may involve video-assisted thoracoscopic surgery (vats) for mediastinal esophageal dissection. usually, vats requires single-lung ventilation and has associated cardiopulmonary morbidity [1-3]. alternatively, transhiatal dissection can be performed, although its complications include vocal cord palsy [4], cardiac arrythmias [5], and increased bleeding [5, 6], the latter associated with mortality after esophagectomy [2]. therefore, the feasibility of mie usi ... | 2011 | 20844900 |
| contrast-enhanced ultrasound for blunt hepatic trauma: an animal experiment. | the aim of this study was to study the value of contrast-enhanced ultrasound (ceus) in the diagnosis of active hemorrhage and intraparenchymal lesions in blunt hepatic trauma versus conventional ultrasound (us). | 2010 | 20837263 |
| establishment of a porcine model for lobar lung auto-transplantation. | the aim of this study was to develop a porcine model of left single lung auto-transplantation. | 2010 | 20832588 |
| reassortant between human-like h3n2 and avian h5 subtype influenza a viruses in pigs: a potential public health risk. | human-like h3n2 influenza viruses have repeatedly been transmitted to domestic pigs in different regions of the world, but it is still uncertain whether any of these variants could become established in pig populations. the fact that different subtypes of influenza viruses have been detected in pigs makes them an ideal candidate for the genesis of a possible reassortant virus with both human and avian origins. however, the determination of whether pigs can act as a "mixing vessel" for a possible ... | 2010 | 20830295 |
| volumetric tracking tool using four-dimensional ct for image guided-radiation therapy. | to analyze lung vessel motion automatically, we developed computerized tracking software for multi-slice ct (msct) data. here, we evaluated the tracking accuracy in images of healthy porcine lungs obtained by 256 multi-slice ct (256-msct). a domestic pig was scanned with 256-msct under free breathing conditions. the 256-msct provides 128-mm scan coverage with a 0.5-mm slice thickness in a single rotation (1.0 s/rot). automatic tracking was performed by template matching, where volumes of interes ... | 2008 | 20821162 |
| new piophilid in town: the first palaearctic record of piophila megastigmata and its coexistence with piophila casei in central spain. | the genus piophila fallén (diptera: piophilidae) is known from only two species: piophila casei (l.) is a major pest, a cosmopolitan species and is commonly used as a forensic indicator, whereas piophila megastigmata mcalpine has until now only been recorded in a natural environment in south africa. the present work reports the first occurrence of p. megastigmata in the palaearctic region from specimens collected by carrion-baited traps throughout different natural habitats of central spain. fur ... | 2011 | 20819151 |
| peptide vaccine candidates against classical swine fever virus: t cell and neutralizing antibody responses of dendrimers displaying e2 and ns2-3 epitopes. | three peptide-based systems integrating b and t antigenic sites of csfv and displaying the b epitopes in fourfold presentation have been designed and produced, and shown to bring about significant enhancements in immunogenicity over the peptides in monomeric form. of the different strategies tested for producing the dendrimeric constructs, stepwise spps using 3,6-dioxaoctanoic acid as flexible, peg-like spacer units at the branching points is clearly advantageous, in particular over ligation in ... | 2011 | 20812373 |
| a study on repair of porcine articular cartilage defects with tissue-engineered cartilage constructed in vivo by composite scaffold materials. | the study was performed to find out a promising injectable composite scaffold for cartilage tissue engineering. by using a composite of allogenous cartilage microparticle acellular tissue matrix (cmactm) and fibrin glue (fg) as injectable scaffold materials, tissue-engineered cartilage was constructed in vivo, and the effects of which on the repair of porcine articular cartilage defects were observed. cmactm was obtained from domestic pigs. the chondrocytes were prepared from experimental mini-t ... | 2010 | 20798625 |
| camp-activated ca2+ signaling is required for cftr-mediated serous cell fluid secretion in porcine and human airways. | cystic fibrosis (cf), which is caused by mutations in cftr, affects many tissues, including the lung. submucosal gland serous acinar cells are primary sites of fluid secretion and cftr expression in the lung. absence of cftr in these cells may contribute to cf lung pathogenesis by disrupting fluid secretion. here, we have isolated primary serous acinar cells from wild-type and cftr-/- pigs and humans without cf to investigate the cellular mechanisms and regulation of fluid secretion by optical i ... | 2010 | 20739756 |
| toxicity of valproic acid in liver slices from sprague-dawley rats and domestic pigs. | the reason for sensitivity to valproic acid (vpa) hepatotoxicity in humans is not known and requires further investigation. we investigated two in vitro animal models that might represent the unpredictably sensitive and the predictably non-sensitive populations of patients. vpa-induced hepatotoxicity was evaluated in vitro using precision-cut liver slices prepared from adult sprague-dawley rats and 4-wk-old domestic pigs. protein synthesis, k(+) retention and cytosolic lactate dehydrogenase leak ... | 1991 | 20732016 |
| durability of the self-approximating translumenal access technique (stat) for potential use in natural orifice translumenal surgery (notes). | the self-approximating translumenal access technique (stat) has been shown to provide a safe and reliable means of abdominal access for natural orifice translumenal endoscopic surgery (notes). however, the feasibility of using stat for translumenal organ resection is unknown. this study aimed to evaluate the technical performance of organ resection using stat, the integrity of the stat gastric tunnel after organ resection, and the postoperative morbidity of organ resection using stat. | 2011 | 20725749 |
| examination of protective effect of ischemic postconditioning after small bowel autotransplantation. | ischemia/reperfusion (i/r) injury is a serious condition that results from some surgical procedures, including intestinal transplantation. ischemic postconditioning is defined as brief periods of reperfusion alternating with reocclusion applied during the early minutes after reperfusion. the objective of this study was to investigate the effect of ischemic postconditioning before small bowel autotransplantation. total orthotopic intestinal autotransplantation was performed in 30 white domestic p ... | 2010 | 20692464 |
| a three-dimensional digital segmented and deformable brain atlas of the domestic pig. | we used high-magnetic field (4.7 t) magnetic resonance imaging (mri) to build the first high-resolution (100 microm x 150 microm x 100 microm) three-dimensional (3d) digital atlas in stereotaxic coordinates of the brain of a female domestic pig (sus scrofa domesticus). this atlas was constructed from one hemisphere which underwent a symmetrical transformation through the midsagittal plane. concomitant construction of a 3d histological atlas based on the same scheme facilitated control of deep br ... | 2010 | 20692291 |
| prevalence of actinobacillus pleuropneumoniae infection in hunted wild boars (sus scrofa) in germany. | actinobacillus pleuropneumoniae is an important pathogen of the porcine respiratory tract. subclinically and chronically infected pigs may enter a permanent carrier state, harboring a. pleuropneumoniae in lungs or tonsils. despite the enormous role of a. pleuropneumoniae in domestic swine production, no published data about prevalence and distribution of a. pleuropneumoniae infections in wild boars (sus scrofa) are available. antibodies against a. pleuropneumoniae have been detected in serum of ... | 2010 | 20688648 |
| experimental study of pancreaticojejunostomy completed using anastomotic chains. | the most difficult, time-consuming, and complication-prone step in pancreaticoduodenectomy is the pancreaticojejunostomy step. the largest disadvantage of this kind of anastomosis is the high incidence of postoperative anastomotic leakage. once pancreatic leakage occurs, the patient death rate can be very high. the aim of this study was to design a pancreaticojejunostomy procedure using anastomotic chains, which results in the cut end of the jejunum being attached to the pancreatic stump without ... | 2010 | 20687743 |
| the rethink project--minipigs as models for the toxicity testing of new medicines and chemicals: an impact assessment. | the objective of the rethink project was to evaluate the potential impact of toxicity testing in the minipig as an alternative approach in regulatory toxicity testing that can contribute to the replacement, refinement and reduction of animal testing (3rs). minipigs are strains of domestic pigs that are markedly smaller than farmyard varieties, and thus better adapted to laboratory housing. the pig closely resembles man in many features of its anatomy, physiology, biochemistry and lifestyle. in p ... | 2010 | 20685395 |
| analysis of 11 tetrameric strs in wild boars for forensic purposes. | str profiling of animal species has a wide range of applications, including forensic identification, wildlife preservation, veterinary public health protection and food safety. we tested the efficacy of a multiplex pcr-based assay including 11 porcine-specific tetrameric strs in a population sample of wild boars (n=142) originating from piedmont (north west italy). multiple deviations from hardy-weinberg expectations were observed, mostly due to a reduction in observed heterozygosity indicative ... | 2010 | 20685189 |
| effect of continuous compressions and 30:2 cardiopulmonary resuscitation on global ventilation/perfusion values during resuscitation in a porcine model. | rescue ventilations during bystander resuscitation, although previously considered essential, interrupt the continuity of chest compressions and might have deleterious effects in basic life support. this study was undertaken to analyze the global ventilation/perfusion values of continuous compressions and 30:2 cardiopulmonary resuscitation to determine the effectiveness for each approach in a porcine model of prolonged bystander cardiopulmonary resuscitation for ventricular fibrillation. | 2010 | 20683258 |
| comparative pathology of select agent influenza a virus infections. | influenza a virus infections may spread rapidly in human populations and cause variable mortality. two of these influenza viruses have been designated as select agents: 1918 h1n1 virus and highly pathogenic avian influenza (hpai) virus. knowledge of the pathology of these virus infections in humans, other naturally infected species, and experimental animals is important to understand the pathogenesis of influenza, to design appropriate models for evaluation of medical countermeasures, and to mak ... | 2010 | 20682805 |
| in vitro permeation of a pegylated naltrexone prodrug across microneedle-treated skin. | microneedles (mn) are a useful tool for increasing skin permeability to xenobiotics. previous research showed marked improvement in the percutaneous flux of naltrexone (ntx) hydrochloride by the use of mn skin pretreatment alone; however, for better therapeutic effect, further enhancement is desired. the goal of this in vitro study was to combine microneedle skin pretreatment with the use of a highly water-soluble pegylated naltrexone prodrug (polyethyleneglycol-ntx, peg-ntx) to investigate its ... | 2010 | 20678989 |
| chondroitin sulfate demarcates astrocytic territories in the mammalian cerebral cortex. | adjacent astrocytes in the grey matter of the mammalian cerebral cortex are organized in a tile-like manner and separated from one another, forming discrete domains named "non-overlapping territories". we have previously reported that an anti-chondroitin sulfate (cs) antibody, cs-56, marks a subpopulation of cortical astrocytes which we named the dandelion clock-like structure (dacs) based on its morphological characteristics. in the present study, we found that another anti-cs antibody (anti-cs ... | 2010 | 20678547 |
| effects of sodium selenite and amiloride on calvarial calcification in closing small cranial defects. | fresh autogenous bone graft is the most preferred osteoplastic material, whether the purpose is cosmetic, psychological, or for the protection of the brain. these grafts are not rejected and do not react immunologically. the aim of this study was to evaluate the efficacy of autogenous fat rolled with bone dust derived from the bur hole in closing small cranial defects. additionally, the authors examined the morphological and biochemical effects of na selenite and amiloride on calvarial calcifica ... | 2011 | 20672896 |
| a tractable experimental model for study of human and animal scabies. | scabies is a parasitic skin infestation caused by the burrowing mite sarcoptes scabiei. it is common worldwide and spreads rapidly under crowded conditions, such as those found in socially disadvantaged communities of indigenous populations and in developing countries. pruritic scabies lesions facilitate opportunistic bacterial infections, particularly group a streptococci. streptococcal infections cause significant sequelae and the increased community streptococcal burden has led to extreme lev ... | 2010 | 20668508 |
| echo-endoscope biplane navigation system for endovascular aneurysm repair. | requirement of contrast medium limits the indication of endovascular intervention to patients with renal insufficiency and allergic reaction. furthermore, landmarks under fluoroscopic guidance do not necessarily encompass the local anatomy of complex aortic lesions. we evaluated the feasibility of a bimodal monitoring system consisting of echocardiography and endoscopy as a more accurate anatomical navigation system for endovascular intervention. | 2010 | 20667361 |
| the effects of rapid enzymatic debridement of deep partial-thickness burns with debrase on wound reepithelialization in swine. | reepithelialization of deep burns requires spontaneous or active removal or debridement of the necrotic eschar, as recently defined by the american burn association. debrase is a bromelain-derived enzymatic preparation that has been shown to result in rapid and selective debridement of human and animal burns. the authors hypothesized that rapid debridement of deep dermal burns with debrase would result in earlier reepithelialization of the remaining dermis in a porcine model. eighty deep dermal ... | 2010 | 20661148 |
| pancreatic islet plasticity: interspecies comparison of islet architecture and composition. | the pancreatic islet displays diverse patterns of endocrine cell arrangement. the prototypic islet, with insulin-secreting beta-cells forming the core surrounded by other endocrine cells in the periphery, is largely based on studies of normal rodent islets. recent reports on large animals, including humans, show a difference in islet architecture, in which the endocrine cells are randomly distributed throughout the islet. this particular species difference has raised concerns regarding the inter ... | 0 | 20657742 |
| tetrahydrolipstatin inhibition, functional analyses, and three-dimensional structure of a lipase essential for mycobacterial viability. | the highly complex and unique mycobacterial cell wall is critical to the survival of mycobacteria in host cells. however, the biosynthetic pathways responsible for its synthesis are, in general, incompletely characterized. rv3802c from mycobacterium tuberculosis is a partially characterized phospholipase/thioesterase encoded within a genetic cluster dedicated to the synthesis of core structures of the mycobacterial cell wall, including mycolic acids and arabinogalactan. enzymatic assays performe ... | 2010 | 20656688 |
| protection of the renal collecting system during radiofrequency ablation with antegrade cold dextrose infusion. | to evaluate the feasibility and efficacy of antegrade renal cooling during radiofrequency (rf) ablation to protect the renal collecting system in pigs. | 2010 | 20651062 |
| establishment of a streptozotocin-induced diabetic domestic pig model and a systematic evaluation of pathological changes in the hard and soft tissue over a 12-month period. | the number of diabetic patients in need of medical treatment is growing steadily. therefore, a diabetic animal model with high degree of similarities with humans, which is suitable for the systematic evaluation of biomaterials and medical devices, is needed. | 2010 | 20636725 |
| micrornaome of porcine pre- and postnatal development. | the domestic pig is of enormous agricultural significance and valuable models for many human diseases. information concerning the pig micrornaome (mirnaome) has been long overdue and elucidation of this information will permit an atlas of microrna (mirna) regulation functions and networks to be constructed. here we performed a comprehensive search for porcine mirnas on ten small rna sequencing libraries prepared from a mixture of tissues obtained during the entire pig lifetime, from the fetal pe ... | 2010 | 20634961 |
| a role for factor xiia-mediated factor xi activation in thrombus formation in vivo. | mice lacking factor xii (fxii) or factor xi (fxi) are resistant to experimentally-induced thrombosis, suggesting fxiia activation of fxi contributes to thrombus formation in vivo. it is not clear whether this reaction has relevance for thrombosis in pri mates. in 2 carotid artery injury models (fecl(3) and rose bengal/laser), fxii-deficient mice are more resistant to thrombosis than fxi- or factor ix (fix)-deficient mice, raising the possibility that fxii and fxi function in distinct pathways. a ... | 2010 | 20634381 |
| does pressure matter in creating burns in a porcine model? | multiple animal models of burn injury have been reported, and only some of these have been fully validated. one of the most popular approaches is burn infliction by direct contact with the heat source. previous investigators have reported that the pressure of application of the contact burn infliction device does not affect the depth of injury. we hypothesized that the depth of injury would increase with increasing pressure of application in a porcine burn model. forty mid-dermal contact burns m ... | 2010 | 20616654 |
| seeding endothelial progenitor cells on a self-expanding metal stent: an in vitro study. | to demonstrate the feasibility of seeding a self-expanding metal stent with endothelial progenitor cells to enhance rapid reendothelialization, which is postulated to prevent local thrombus formation and restenosis after vascular intervention. | 2010 | 20610181 |
| different fusion configurations of evolutionarily conserved segments in karyotypes of potamochoerus porcus and phacochoerus africanus. | the karyotype of the red river hog potamochoerus porcus (2n = 34) differs from that of the domestic pig by the presence of 2 fusion chromosomes homologous to pig chromosomes 13/16 and 15/17. moreover, chromosomes corresponding to pig chromosomes 13/16 and 1 are both acrocentric. hybridization with region-specific painting probes confirmed tandem fusion of pig chromosomes 13 and 16, and a pericentric inversion of the pig chromosome 1p equivalent in p. porcus. the chromosome complement of the wart ... | 2010 | 20606389 |
| seroprevalence and risk factors associated with toxoplasma gondii in domestic pigs from spain. | serum samples from 2970 (1400 sows, 1570 fattening) pigs, from 100 farms in the 10 main swine production regions in spain were tested for antibodies against t. gondii by the modified agglutination test (mat). antibodies to t. gondii (mat 1:25 or higher) were detected in 492 pigs (16.6%, 9.7% in fattening pigs and 24.2% in sows). the herd prevalence was 85.0% (95% ci: 78-92) and within-farm prevalence ranged from 2.9% to 92.8% (median=17.6%). statistically significant differences were observed am ... | 2010 | 20601110 |
| endoscopic full-thickness resection and defect closure in the colon. | endoscopic full-thickness resection (eftr) is a minimally invasive method for en bloc resection of gi lesions. | 2010 | 20598252 |
| developmental stage-specific imprinting of ipl in domestic pigs (sus scrofa). | imprinted in placenta and liver (ipl) gene has been identified as an imprinted gene in the mouse and human. its sequence and imprinting status, however, have not been determined in the domestic pigs. in the present study, a 259 base pair-specific sequence for ipl gene of the domestic pig was obtained and a novel snp, a t/c transition, was identified in ipl exon 1. the c allele of this polymorphism was found to be the predominant allele in landrace,yorkshire, and duroc. the frequency of cc genoty ... | 2010 | 20589073 |
| fourier domain pump-probe optical coherence tomography imaging of melanin. | we report the development of a two-color fourier domain pump-probe optical coherence tomography (ppoct) system. tissue phantom experiments to characterize the system performance demonstrated imaging depths in excess of 725 microm, nearly comparable to the base optical coherence tomography system. ppoct a-line rates were also demonstrated in excess of 1 khz. the physical origin of the ppoct signal was investigated with a series of experiments which revealed that the signal is a mixture of short a ... | 2010 | 20588366 |
| how pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane. | mammalian sperms are activated in the oviduct. this process, which involves extensive sperm surface remodelling, is required for fertilization and can be mimicked under in vitro fertilization conditions (ivf). | 2010 | 20585455 |
| negative intratracheal pressure produced by esophageal detector devices causes tracheal wall collapse in a porcine cardiac arrest model. | esophageal detector devices (edds) impose negative pressure on the trachea or esophagus to verify endotracheal tube (ett) position. in cardiac arrest, the smooth muscle of the lower esophageal sphincter relaxes in a time-dependent and irreversible manner. if relaxation also occurs in the muscular posterior tracheal wall, it could predispose tracheal invagination or collapse with negative pressure, potentially yielding false-negative (tracheal ett, edd indicates esophagus) results. we compared 3 ... | 2010 | 20584869 |
| depth of tissue ablation and residual thermal damage caused by a pixilated 2,940 nm laser in a swine skin model. | the purpose of this study was to assess the effects of fluence, pulse stacking, and multiple passes on the depth of injury caused by a fractionated er:yag laser in an in vivo farm pig model. design/material/methods: a fractionated 2,940 nm er:yag laser (pixel, alma lasers, caesarea, israel) was applied to the flank skin of a yorkshire cross pig. the 11 mmx11 mm handpiece was comprised of either 49 or 81 microbeams (200 microm diameter), depending on the tip configuration. there were six differen ... | 2010 | 20583246 |
| anterior shear strength of the porcine lumbar spine after laminectomy and partial facetectomy. | degenerative lumbar spinal stenosis is the most common reason for lumbar surgery in patients in the age of 65 years and older. the standard surgical management is decompression of the spinal canal by laminectomy and partial facetectomy. the effect of this procedure on the shear strength of the spine has not yet been investigated in vitro. in the present study we determined the ultimate shear force to failure, the displacement and the shear stiffness after performing a laminectomy and a partial f ... | 2010 | 20582709 |