Publications

transient expression of the coat protein of sugarcane mosaic virus in sugarcane protoplasts and expression in escherichia coli.	the coat protein (cp) of strain sc of sugarcane mosaic virus (scmv-sc) was expressed transiently in sugarcane protoplasts after electroporation with one of two plasmids encoding the cp gene. the cp gene was fused with either the cauliflower mosaic virus 35s promoter or the synthetic monocotyledon promoter "emu". the coat protein gene was also inducibly expressed in escherichia coli when fused to the trc promoter. the protein expressed in both systems had the same electrophoretic mobility and ant ...	1992	1642548
immunochemical tests of potential fertility.	there are many methods of determining the physiological basis of cyclical periods of potential fertility: calculations, sensations, or physical or chemical biosensors. this article reviews the development of self-tests of potential fertility using immunoassay of hormone metabolites in urine. indices of potential fertility in urine are the concentration of principal metabolites of steroid hormones and luteinizing hormone (lf). the ovaries produce an estimated 50-800 mcg of estradiol/day, the most ...	1992	1633952
strain dependency of cell-type specificity and onset of lymphoma development in emu-myc transgenic mice.	c-myc is a nuclear proto-oncogene that, when activated, induces malignancies in a variety of tissues. most murine plasmacytomas and human burkitt's lymphomas have been shown to carry a chromosomal translocation involving c-myc and immunoglobulin genes. to study genetic or epigenetic factors that affect myc-induced lymphoid cell tumors, we previously introduced the emu-myc delta gene lacking its own promoter and first exon into two inbred strains of mice, c57bl/6 and c3h/hej. we observed three ch ...	1992	1582889
urinary tuberculosis: experience of a teaching hospital in syria.	this study was designed to show our experience of urinary tuberculosis in one of the large teaching hospitals in syria. it was a prospective study involving 48 patients (29 males and 19 females) with confirmed tuberculous lesions in the urinary tract. the study period was between 1982-1987. the presenting symptoms were protean, and there were often delays between onset of symptoms and eventual diagnosis. the highest age incidence was in the second and fourth decades. beside the suggestive clinic ...	1992	1459824
down-regulation of c-myc antigen expression in lymphocytes of emu-c-myc transgenic mice treated with anti-c-myc dna methylphosphonates.	in transgenic mice bearing a murine immunoglobulin enhancer/c-myc fusion transgene (emu-myc), it was found that antisense dna methylphosphonates targeted against c-myc mrna inhibited production of c-myc protein in peripheral lymphocytes. the decrease in protein was measured 3-4 h after i.v. administration of a 300-nmol dose. c-myc was detected by immunofluorescence of fixed cells stained with an anti-c-myc antiserum. in addition, dna methylphosphonates did not induce acute toxicity following i.v ...	1992	1458461
reference serum biochemical values for emus and ostriches.	reference serum biochemical values were determined in blood samples from 15 male, 18 female, and 4 unsexed emus (dromaius novaehollandiae) 1 to 48 months old. serum biochemical values also were obtained for 19 male, 26 female, and 4 unsexed ostriches (struthio camelus) 1 to 60 months old. parametric (mean +/- 2 sd) and non-parametric (fifth to 95th percentile) reference ranges and linear trends as influenced by age were determined for enzyme activities and concentrations of glucose, inorganic ph ...	1992	1456516
inhalation doses for aboriginal people reoccupying former nuclear weapons testing ranges in south australia.	the australian government plans to rehabilitate the former nuclear weapons testing ranges at maralinga and emu in south australia and to allow access to the areas for the pitjantjatjara (aboriginal) people who are the traditional owners of the land. the major radiological hazard posed by reoccupation of the ranges arises from inhalation of residual plutonium. the committed effective dose that these people might receive from inhalation on reoccupation of the lands is computed for a range of sites ...	1992	1428883
assessment of potential doses at the maralinga and emu test sites.	an assessment has been undertaken of potential doses to future aboriginal inhabitants of the maralinga and emu areas of south australia, where nuclear weapons tests in the 1950s and 1960s have resulted in residual radioactive contamination. radioactive material due to this program of tests and other experiments is still detectable several tens of kilometers from some of the test sites. continued occupancy by individuals following an aboriginal lifestyle could give rise to annual effective dose e ...	1992	1428882
eastern equine encephalitis in a flock of emus (dromaius novaehollandiae).	eastern equine encephalitis (eee) was diagnosed in a flock of emus in southeastern louisiana. the outbreak involved juvenile and adult breeders ranging in age from 20 to 36 months, with an attack rate of 76% and a case fatality rate of 87%. the diagnosis was confirmed by isolation and characterization of the viral agent, and by detection of eee antibody in two recovered emus. high mortality was preceded by marked depression, hemorrhagic diarrhea, and emesis of blood-stained ingesta. on postmorte ...	1992	1417619
estrone 3-glucuronide chemiluminescence immunoassay (lia) and 17beta estradiol radioimmunoassay (ria) in the monitoring of superovulation for in vitro fertilization (ivf): correlation with follicular parameters and oocyte maturity.	many works in the literature of the last years had reported that urinary approach to superovulation study is a suitable method to evaluate ovarian response to pharmacological stimulation. before applying urinary determination of hormonal levels with a chemiluminescence immuno assay (lia) method in early morning urine (emu) samples, we had studied the correlation of ria-lia procedures with reference to follicular volumes at hcg day and to recovered oocyte maturity; in fact follicular growth and o ...	2013	1338258
urinary protein/creatinine index in follow up of patients with wilms' tumour after nephrectomy.	the protein/creatinine index (p/c) was determined in early morning urine (emu) samples from available patients with wilms' tumour who had had a nephrectomy and whose diagnosis had been made between january 1970 and december 1989. clinical details were obtained by case note review. results were obtained from 36 boys and 40 girls. the mean interval between nephrectomy and measurement of the emup/c was 9.0 years (2-23). eleven patients had a emup/c greater than 20 mg/mmol (normal range less than 20 ...	1992	1336953
studies on the apoproteins of the major lipoprotein of the yolk of hen's eggs. i. isolation and properties of the low-molecular-weight apoproteins.	in a continuing study of protein-lipid interactions in egg yolk, the total apoprotein mixture (i.e. the 'apovitellenins') from the high-lipid, low-density lipoprotein (density 0.97 g/ml) of the yolk from hen's eggs has been isolated in a soluble form. by gel-filtration chromatography in 6m urea the mixture has been separated into several fractions from which three new low-molecular-weight proteins (i, ia, and ii), making up about 30% of the total, have been isolated. the most plentiful of these ...	1975	1167127
response to co2 of intrapulmonary chemoreceptors in the emu.	we studied discharge frequencies of 12 intrapulmonary chemoreceptors in the paleopulmonic lung of an emu (dromiceius novaechollandiae) during unidirectional, artificial ventilation when step changes and static co2 concentrations were given. discharge frequency in afferent neurons from the receptors increased as intrapulmonary co2 decreased. the response of the receptors to various static intrapulmonary co2 concentrations was similar to that previously demonstrated for the duck and chicken. the m ...	1976	1019429
chemoreceptors in the paleopulmonic lung of the emu: discharge patterns during cyclic ventilation.		1976	1013516
primary structure of apovitellenin i from hen egg yolk and its comparison with emu apovitellenin i.	the amino acid sequence of apovitellenin i from hen egg yolk has been determined using both automatic and manual procedures; it comprises 82 residues. hen apovitellenin shows considerable homology with emu apovitellenin i which contains 84 residues. besides two deletions in the sequence, the hen protein differs in 28 positions from the emu protein; 26 of these positions may have arisen from single base changes. the changes are largely conservative ones, which suggest that the structure and funct ...	1976	988817
editorial: dr. edwards' emu.		1976	937161
calf thymus alkaline phosphatase. ii. interaction with detergents.	1. a number of detergents were used to dissolve calf thymus plasma membranes rich in alkaline phosphatase (orthophosporic-monoester phosphohydrolase (alkaline optimum), ec 3.1.3.1) activity. 2. the stokes' radius (r) of alkaline phosphatase in each detergent was measured by gel filtraton. the size of the solubilized enzyme varied from r = 6.2 nm in sodium cholate to r = 8.3 nm in berol emu-043. with n-alkylsulphates, the apparent size increased with alkyl chain length, with r = 6.4 nm (c9) and ...	1977	831832
immunochemical analyses of membrane-bound complement. detection of the terminal complement complex and its similarity to "intrinsic" erythrocyte membrane proteins.	(1) membranes of sheep erythrocytes lysed with antibody and human or rabbit complement were solubilized in non-ionic detergents (triton x-100 or berol emu-043) and analysed immunochemically using antisera directed against individual complement components. the precipitation behaviour of membrane-bound c3, c5, c6 and c9 components of complement was examined by immuno-double diffusion, rocket- and crossed immunoelectrophoresis performed in agarose gels containing 1% non-ionic detergent. (2) membran ...	1975	809065
verminous encephalitis in the emu.		2003	567056
lipid-protein globules of avian egg yolk. isolation and properties of globules stable in concentrated sodium chloride solution.	a new type of globular particle, the 'insoluble yolk globule', was isolated from the egg yolk of three avian species (hen, duck, and emu) by centrifugation or gel-filtration chromatography. these globules are stable in nacl and urea solutions at concentrations that dissolve or disrupt other constituents of yolk, the isolated globules are about 1% of the dry yolk of hen's and duck's eggs but about 8% emu's-egg yolk. most of these globules are less than 2 micrometer in diameter. electron micrograp ...	1977	563717
urinary hydroxyproline excretion in carcinoma of the prostate. a comparison of 4 different modes of assessment and its role as a marker.	urinary hydroxyproline (hp) excretion has been estimated without prior dietary restriction in 33 patients with carcinoma of the prostate and expressed as either 24-h hp output or as the hydroxyproline/creatinine (hp/cr) ratio in 24-h urine sample, an early morning urine sample or a spot urine sample. the early morning urine hydroxyproline/creatinine ratio (emu hp/cr) appears to be the most accurate and avoids the disadvantages of formal dietary restriction and prolonged urine collection. the res ...	1979	534843
quantitative immunoelectrophoresis of proteins in human erythrocyte membranes. analysis of protein bands obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.	1. we have defined conditions that permit quantitative immunoelectrophoresis in agarose gels of dodecyl sulfate-solubilized erythrocyte membrane proteins. 2. using human serum albumin, transferrin, mn-glycoprotein (glycophorin) and crude spectrin as test proteins, we found that accurate analyses are possible if samples and gels are 1% in non-ionic detergent (berol emu-043) or triton x-100) and if no more than 100 nmol free dodecyl sulfate is applied per sample. 3. dodecyl sulfate treated membran ...	1975	52375
immunoabsorption of membrane-specific antibodies for determination of exposed and hidden proteins in human erythrocyte membranes.	human erythrocyte membrane proteins solubilized with the non-ionic detergent berol emu-043 have been characterized by crossed immunoelectrophoresis with rabbit antibodies raised against the membrane material. three out of sixteen membrane-specific immunoprecipitates disappeared when the antisera were first absorbed with intact erythrocytes. this finding indicates that three antigens are exposed on the outside of the erythrocyte membrane. one of these antigens showed acetylcholinesterase activity ...	1975	49198
reactivity and ionization constants of the lysine residues in apovitellenin i of emu egg yolk low-density lipoprotein by competitive labelling.	competitive labelling with[14c]acetic anhydride over a range of ph values has been used to explore the surface topography of the apovitellenin i moiety in emu egg yolk low-density lipoprotein. the reaction of the lysine xi-amino groups with acetic anhydride has been related to ph in a set of titration curves; from these, the reactivities relative to alanine and the ionization constants of all but the amino terminal lysines have been determined. all lysines have near normal pka values around 10, ...	1975	5069