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timing of a single daily meal and diel variations of serum thyroxine, triiodothyronine and cortisol in goldfish carassius auratus. 19817196481
fine structural characteristics of the hepatic sinusoidal walls of the goldfish (carassius auratus).fine structural characteristics of hepatic sinusoidal walls of the goldfish were described. kupffer's stellate cells (macrophages) are few in number in the sinusoidal endothelial lining of this fish. very rarely macrophages, whose small cytoplasmic protrusion is sometimes inserted between two endothelial cells, are localized in the sinusoidal lumen in contact with the endothelial cell. typical desmosomes are frequently seen between the two endothelial cells, between the endothelial cell and the ...19807192975
the relationship between the activity of splenic enzymes and the immune response in the goldfish, carassius auratus. i. preliminary experiments with human a1 secretor's saliva and a1 rbc. ii. preliminary enzyme experiments using alpha-gpd and ldh.1. goldfish, carassius auratus, were injected with two antigenic substances a1 secretor's saliva or a1 rbc. 2. the goldfish produced significant antibody titers which continued for 10-12 weeks when maintained at 28 degrees c. 3. analysis of enzymatic activities of soluble and particulate alpha-gpd and ldh from skeletal muscle and spleen showed significant differences between and within tissues.19827172634
biochemical bases for environmental adaptation in goldfish (carassius auratus l.): resistance to ammonia.goldfish specimens were exposed for 24-48 hr to 20, 40, 600, and 2500 micrograms n/liter unionized ammonia (uia). this treatment causes, in the brain, increases in total ammonia, glutamine, lactate, and succinate and decreases in glutamate, glucidic stores, total nadh, and atp. most of these effects have already been reported in ammonia-treated trout. it is therefore suggested that the cerebral biochemical mechanisms of ammonia toxicity in these two species are fundamentally the same. the most i ...19827169039
morphologic evidence for seasonal changes in the pineal organ of the goldfish, carassius auratus; a quantitative study.a quantitative morphologic study of photoreceptor cells in pineal organ of the goldfish was conducted over a period of one year in which the photoperiod-temperature regime simulated the natural environment. statistically significant seasonal differences were found in cell size, nuclei, nucleoli, mitochondria, endoplasmic reticulum, and golgi bodies. peak values generally occurred during the fall and winter months, while minimum values coincided with the reproductive spring and summer seasons. hi ...19827163615
structure of the mitral cell in the olfactory bulb of the goldfish (carassius auratus).the mitral cell in the olfactory bulb of the goldfish was examined by means of light microscopy, high-voltage electron microscopy, and conventional electron microscopy. mitral cells are located rather diffusely throughout the glomerular and plexiform layers. they do not make their own discrete layer. the cell bodies are rounded or triangular, and are about 10-25 micrometers in diameter. in golgi-impregnated material, thick cylindrical dendrites can be seen arising from the cell bodies and branch ...19827161415
neurohypophysial hormonal control of cortisol secretion in the teleost carassius auratus. 19827160609
uptake and toxicity of toxaphene to cell cultures derived from goldfish (carassius auratus). 19827115996
the effects of constant light and constant darkness on daily changes in the morphology of the pineal organ in the goldfish, carassius auratus.the fine structure of photoreceptor cells in the pineal organ of the goldfish was found to vary quantitatively over a 24-hour period. stereological analysis revealed significant daily changes in the volume of the cell and inner segment, nuclear volume and nucleolar diameter, volume of endoplasmic reticulum and golgi bodies, area of both rough and smooth endoplasmic reticulum, and number of vesicles associated with each golgi body. peak values of these variables occurred either during the dark ph ...19827108508
habituation of fright and arousal responses in the teleosts carassius auratus and rutilus rutilus.the habituation of fright and arousal responses of goldfish (carassius auratus) and roach (rutilus rutilus) to the repeated operation of a plunger in the water was studied. the early response was of fright, which habituated, and the response characteristic of arousal appeared, which then habituated as well. longer intervals between stimuli required more presentations of the stimulus for habituation to occur in goldfish. roach required more presentations of the stimulus than goldfish for the resp ...19827096683
nerve growth factor stimulates neurite outgrowth from goldfish retinal explants: the influence of a prior lesion.goldfish (carassius auratus) retinal explants, whose ganglion cells were 'primed' in vivo by an optic nerve crush at varying intervals prior to culture, were found to respond by enhanced neurite outgrowth to low (ng/ml) concentrations of nerve growth factor (ngf). this in vitro response was dose-dependent and specific for ngf. the spontaneous fiber outgrowth which normally occurs in vitro in response to optic nerve lesion without exogenous ngf in the medium could be reduced by approximately 80% ...19827093729
isozyme expression in f1 hybrids between carp and goldfish.interspecific genetic differences in malate dehydrogenase (mdh), lactate dehydrogenase (ldh), superoxide dismutase (sod), and esterase (est) isozymes in carp (cyprinus carpio) and goldfish (carassius auratus) were used to examine the allelic expressions in the hybrid between these species. a unique liver sod and muscle ldh phenotype unambiguously identifies all presumed hybrid individuals. there was no evidence of f2 or backcross phenotypes in hybrid individuals. liver mdh and est phenotypes in ...19827092795
the effects of constant light and constant darkness on the pineal organ of the goldfish, carassius auratus.goldfish (carassius auratus) were subjected to both long- and short-term (1, 3, 6, and 170 days) constant light or constant darkness to determine the effects of environmental lighting on the morphology of photoreceptor cells in the pineal organ. morphometric analysis of electron micrographs from each experimental group suggested that the effects of constant darkness are morphologically expressed only after long-term (6 months) exposure. these included an increase in the volume of photoreceptor a ...19827077259
morphology of radial glia, ependymal cells, and periventricular neurons in the optic tectum of goldfish (carassius auratus).there are three populations of cells in the deep layers of the optic tectum in a normal adult goldfish: the periventricular neurons, the ependymal cells, and the radial glia. the characteristic morphological features which distinguish the three cell populations are examined at light and electron microscopic levels in the present work. a radial glial cell has a deeply invaginated nucleus located in a subependymal layer. its cytoplasm contains mitochondria with 35-nm dark granules and 20-nm microt ...19827076888
ultrastructure of scales in a teleost (carassius auratus l.) after use of rapid freeze-fixation and freeze-substitution.new data on the ultrastructural features of the elasmoid scales of carassius auratus have been obtained by use of rapid freezing with subsequent freeze-substitution in anhydrous solvents. these are compared with the results obtained using conventional aqueous fixatives. the external layer of the scales is composed of randomly oriented collagen fibres. in the first stages of mineralization, mineral deposits are located in the interfibrillary substance where dense granules appear to be active site ...19827066978
a technique for assaying the activities of alpha-gpd, ldh, and mdh in the teleost, the goldfish, carassius auratus.1. a spectrophotometric assay using rho-iodo-nitro-triphenyl tetrazolium chloride as the indicator was developed for splenic tissue in the goldfish carassius auratus. 2. this technique permits a rapid test for the activities of the enzymes lactic dehydrogenase, malic dehydrogenase, and alpha-glycerophosphate dehydrogenase which are indicative of the relative activities of various metabolic pathways.19827060353
anaerobic metabolism of red skeletal muscle of goldfish. carassius auratus (l.):mitochondrial produced acetaldehyde as anaerobic electron acceptor. 19827040103
forebrain connections in the goldfish support telencephalic homologies with land vertebrates.horseradish peroxidase injections into dorsomedial and dorsolateral regions of the goldfish (carassius auratus) telencephalon demonstrate, by retrograde cell labeling, that the teleost telencephalon receives a pattern of projections from the thalamus remarkably similar to those of land vertebrates. the evidence provides support for a homology between the dorsomedial region and the corpus striatum of land vertebrates and a homology between two dorsolateral regions and the dorsal and medial palliu ...19816971493
responses of msh and prolactin cells to 5-hydroxytryptophan (5-htp) in amphibians and teleosts.injection of 5-hydroxytryptophan (5-htp), a precursor of serotonin, induces dispersion of melanin in the amphibians, pleurodeles waltlii (urodela) and xenopus laevis (anura), in the goldfish, carassius auratus, and in the carp, cyprinus carpio. it is accompanied by a dispersion of erythrophore pigments. in the pituitaries of pleurodeles and goldfish, a stimulation of msh cells, characterized by a significant nuclear hypertrophy, is also observed; in carassius, msh cells may become degranulated. ...19806967354
visualization of microtubules of cells in situ by indirect immunofluorescence.microtubule staining patterns can be visualized within cells in situ on the surface of fish scales from the squirrel fish, holocentrus ascensionis, and the common goldfish, carassius auratus, after incubation with antibodies to sea urchin tubulin and fluorescein-labeled goat antibodies to rabbit immunoglobulin g. chromatophores in situ from both species reveal a radial microtubule framework that orients the alignment of pigment granules. innervating fibers of erythrophores on the h. ascensionis ...19806935678
ultrastructural localization of delta 5-3 beta-hydroxysteroid dehydrogenase in the interrenal cells of the goldfisch (carassius auratus).the ultrastructural localization of the enzyme delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta-hsd) was studied in the goldfish interrenal cells by using the potassium ferricyanide method. immersion fixation in a mixture of 1% paraformaldehyde and 0.25% glutaraldehyde results in a consistently good ultrastructural preservation and enzyme localization. a precipitate of copper ferrocyanide indicating the localization of 3 beta-hsd activity was observed in close vicinity to the smooth endoplasm ...19806933018
permanent cell lines from erythrophoromas in goldfish (carassius auratus).attempts to establish permanent cell lines from spontaneous erythrophoromas (tumors derived from red pigment cells or erythrophores) of goldfish were made with the use of biopsy specimens from 12 tumors in 10 fish. three cell lines were established that grew in vitro in synthetic medium (l-15 or dulbecco's modified eagle's minimum essential medium) supplemented with 20% fetal bovine serum for more than 8 months. one of these lines (gem-81) with high proliferative activity was cultured for over 2 ...19806928999
relative in vitro effectiveness of 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one and other pregnene derivatives on germinal vesicle breakdown in oocytes of ayu (plecoglossus altivelis), amago salmon (oncorhynchus rhodurus), rainbow trout (salmo gairdneri), and goldfish (carassius auratus).the relative effectiveness of several pregnene derivatives, which had previously been identified in the ovaries of ayu (plecoglossus altivelis), on germinal vesicle breakdown (gvbd) was investigated in vitro using folliculated oocytes of four species of teleosts, ayu, amago salmon (oncorhynchus rhodurus), rainbow trout (salmo gairdneri), and goldfish (carassius auratus). although some species differences existed in the relative effectiveness of each steroid on gvbd, 17 alpha, 20 beta-dihydroxy-4 ...19836884756
the biotransformation of three 14c-labelled phenolic compounds in twelve species of freshwater fish.1. the urinary and biliary excretion of 14c-labelled m-cresol, 1-naphthol and o-chlorophenol were investigated in 12 species of freshwater fish (bitterling, rhodeus sericeus amarus; bream, abramis brama; crucian carp, carassius carassius; goldfish, carassius auratus; gudgeon, gobio gobio; guppy, poecilia reticulata; minnow, phoximus phoximus; perch, perca fluviatilis; roach, rutilus rutilus; rudd, scardinius erythropthalmus; three-spined stickleback, gasterosteus aculeatus; tench, tinca tinca) w ...19836880238
species differences, influence of dose and application on biotransformation of phenol in fish.1. the metabolism of phenol in goldfish (carassius auratus), rainbow trout (salmo gairdneri) and goldenorfe ide (leuciscus ideus melanotus) was compared. all three species produced phenyl sulphate, quinol sulphate and phenyl glucuronide. 2. the dose dependence of metabolism of phenol in goldfish was investigated. when the concn. of phenol was increased from 0.2 to 2 mg/l medium, that glucuronylated increased from 7 to 16% and that sulphated decreased from 63 to 47%. 3. the influence of mode of e ...19836880237
depression of sodium, chloride and calcium ions in the plasma of goldfish (carassius auratus) and immature freshwater- and seawater-adapted eels (anguilla anguilla l.) after acute administration of salmon calcitonin.goldfish showed a significant reduction in their plasma sodium and calcium levels 1 h after receiving 50 i.u. calcitonin per kg body weight. when 100 i.u. calcitonin were injected there was a significant fall in circulating levels of both sodium and calcium and also of chloride ions compared with those found in untreated control animals. administration of calcitonin to immature eels adapted to either freshwater or seawater conditions showed no significant change in plasma ion composition at dose ...19836875432
immunocytochemistry of gonadotropic cells in the pituitary of some teleost species.an antiserum (anti-sgth) raised against salmon gonadotropin (sg-g100) was tested on pituitary sections of sarotherodon mossambicus, carassius auratus, anguilla anguilla (intact and estradiol-treated to induce the development of the gonadotropic (gth) cells), salmo trutta fario, and a protandric marine teleost (sarpa salpa, sparidae). using an immunoenzymologic technique, anti-sgth stained gth and thyrotropic (tsh) cells in the species studied. anti-sgth stained the granulated gth cells in s. tru ...19836862173
hydrolysis of the biliary glucuronic acid conjugate of phenol by the intestinal mucus/flora of goldfish (carassius auratus).1. an investigation was carried out on the hydrolytic activity of the intestinal mucus/flora on phenylglucuronide in the bile of goldfish. 2. approximately 79% of the total phenylglucuronide (3.4 mumol) in the bile was hydrolysed after 16 h incubation with the intestinal mucus/flora. 3. of the total phenol in the aquarium water of goldfish exposed previously to phenol for 48 h, 41% was found to be phenylglucuronide when fish were placed in a phenol-free medium and were dosed hourly for 8 h with ...19836858197
characteristics of the hepatic monooxygenase system of the goldfish (carassius auratus) and its induction with beta-naphthoflavone.treatment of goldfish with beta-naphthoflavone (ip) resulted in induction of hepatic monooxygenase activity in a dose-dependent manner. levels of 7-ethoxyresorufin-o-deethylase and mexacarbate nadph-dependent oxidative metabolism as well as cytochrome(s) p-450 were increased. other substrates which have been associated with 3-methylcholanthrene (3-mc)-type induction in mammals were unchanged as was delta-aminolevulinic acid synthetase. enzyme activities were inhibited by carbon monoxide and pipe ...19836857672
chromium and potassium accumulation influenced by body weight in goldfish (carassius auratus). 19836850119
drinking induced by angiotensin ii in fishes.among 20 species of freshwater fishes examined, pseudorasbora parva, rhodeus ocellatus, cobitis anguillicaudatus, carassius auratus, oryzias latipes, gambusia affinis, and gyrinocheilus anymonieri were found to drink water like seawater fishes, while 13 remaining species did not drink. for fish species found exclusively in fresh water, angiotensin ii (aii) treatment did not induce drinking. in contrast, those freshwater fishes which survive in estuarine brackish water (leuciscus hakonensis, c. c ...19836840524
morphologic changes in teleost primary and secondary retinal cells following brief exposure to light.light adaptive morphologic changes in the teleost retina, such as movements of rods, cones, and epithelial pigment and spinule formation on horizontal cell terminals, are normally associated with continual exposure to light. depending on a variety of factors these processes are generally completed within 30-60 min. in this report we show that a brief exposure to light (1-2 min) preceded and followed by darkness is sufficient to elicit these changes in four species of teleost; the trout (salmo ga ...19836826311
[1st immunocytochemical application of an anti-dopamine antibody in the study of the central nervous system].an antibody against dopamine was raised in rabbits by means of an immunogen constituting dopamine coupled to bovine serum albumin by formaldehyde. these antibodies were applied to frozen sections of goldfish (carassius auratus) brain fixed by 4% paraformaldehyde. the localisation of immunoreactive structures corresponded to regions rich in dopamine detected by histofluorescence. the specificity of this antiserum tested with the aid of dopamine analogues coupled with a carrying protein different ...19826820314
growth zone of the juvenile goldfish retina revealed by fluorescent flat mounts.the retinas of juvenile goldfish (carassius auratus) were processed for fluorescence microscope studies with flat mounts. the fish were reared in a laboratory aquarium for 1-3 months after either one class of retinal dopaminergic (da) cells or indoleamine-accumulating (ia) cells had been destroyed by intravitreal injection of 6-hydroxydopamine (6-ohda; 2.5 micrograms) or 5,7-dihydroxytryptamine (5,7-dht; 2.5 micrograms). the fish grew 10-12 cm in body length on the average for 3 months. the reti ...19826813509
resonance raman microscopy of rod and cone photoreceptors.we have constructed a raman microscope that has enabled us to obtain resonance raman vibrational spectra from single photoreceptor cells. the laser beam which excites the raman scattering is focused on the outer segment of the photoreceptor through the epiillumination system of a light microscope. raman scattering from the visual pigment in the photoreceptor is collected by the objective and then dispersed onto a multichannel detector. high-quality spectra are recorded easily from individual out ...19826809771
the effect of temperature on the hepatic catabolism of testosterone in the rainbow trout (salmo gairdneri) and the goldfish (carassius auratus). 19806777240
[melanotropic hormone of goldfish (carassius auratus l.): radioimmunologic comparison with synthetic alpha-msh].alpha msh from the neuro-intermediate lobe of the pituitary gland of the goldfish displays a competitive behaviour in an ria for synthetic alpha msh. the inhibition curves from neuro-intermediate homogenates parallel the standard curve (p < 0,01) but two major pools of immunoreactive peptides have been separated by sephadex gel chromatography. the neuro-intermediate lobe contains 480 +/- 56 ng (s.e.m.) of immunoreactive alpha msh.19806777055
ruffed cell: a new type of neuron with a distinctive initial unmyelinated portion of the axon in the olfactory bulb of the goldfish (carassius auratus): ii. fine structure of the ruffed cell.the fine structure and synaptic features of the ruffed cell, especially those of the initial unmyelinated portion of its axon (ip), were investigated by means of electron microscopy. the round or oval cell body is 10 to 20 micrometers in diameter. near the cell body, there is a specialized region of the ip, about 20 to 40 micromerters in diameter, which consists of branched protrusions from the ip. many neuronal processes end on these protrusions, and the whole assembly reminds one of a bird's n ...19806776163
stress fibers in cells in situ: immunofluorescence visualization with antiactin, antimyosin, and anti-alpha-actinin.stress fiber-like patterns are visualized by indirect immunofluorescence in scleroblasts (fibroblasts) in situ on the scale of the common goldfish, carassius auratus, using an affinity-purified antiactin, antimyosin, and anti-alpha-actinin. these fibers demonstrate the classical convergent and parallel patterns exhibited by stress fibers in tissue culture cells. because the dimensions, the composition, and the pattern of distribution of these cytoplasmic fibers correspond well with those of stre ...19826749863
influences of norepinephrine and alpha-adrenergic mechanisms on gonadotropin secretion in female goldfish, carassius auratus.intraperitoneal or intraventricular (3rd cranial ventricle) injection of norepinephrine (ne) caused an increase in serum gonadotropin (gth) concentrations in female goldfish at times of the year when the ovaries were regressed or at early stages of recrudescence, but had no influence at other stages of the ovarian cycle. in a previous report it was shown that clonidine (alpha-agonist) increased serum gth levels in fish at early or mid-ovarian recrudescence [j. p. chang, a. f. cook, and r. e. pet ...19846745634
prostaglandin synthesis in goldfish heart, carassius auratus.potential precursors for prostaglandin (pg) synthesis were measured in goldfish heart and skeletal muscle by gas chromatography. heart tissue contained docosahexaenoic, arachidonic, eicosapentaenoic, and eicosatrienoic acids in concentrations of 3223 +/- 128, 1216 +/- 7.8, 260 +/- 72.8, and 250 +/- 14 ng/mg wet wt, respectively. 14c-labeled substrates were examined for their ability to be converted to prostaglandins. eicosatrienoic and docosahexaenoic acid were not synthesized into prostaglandin ...19846735165
[the primary structure of hemoglobin from goldfish (carassius auratus)].the primary structures of the alpha- and beta-chains from goldfish hemoglobin are given. the globin chains were separated by gel filtration after air-oxidation of globin. after chemical and enzymatical cleavage of the chains, the peptides were isolated by gel filtration and ion exchange chromatography on dowex. the fish-chains have one residue more than the human chains. the alpha-chain is acetylated at the amino-terminal residue and has no cysteine. compared with the human chains there are 66 a ...19846714939
effects of crude dubai oil on salmo gairdneri rich. and carassius auratus l. 19846713130
synaptic contacts between bipolar and photoreceptor cells in the retina of callionymus lyra l.a combined light and electron microscopic study of golgi-impregnated retinas of the marine teleost callionymus lyra l. revealed mixed bipolar cells (m types) contacting rods and cones and pure cone bipolar cells (c types). five types of mixed bipolar cells can be differentiated on the basis of their synaptic contacts. two out of the five mixed bipolar cell types contact double cones, single cones, and rods (mixed, dark, pale, single [mdps and midget-mdps]). their endbuds make narrow cleft juncti ...19846707252
development of retinal monoamine neurons in larval goldfish: a histofluorescence study.the emergence of retinal monoamine neurons was explored in embryonic and larval goldfish (carassius auratus) by means of a histofluorescence technique. the high-affinity uptake capacity of dopamine (da) and indoleamine-accumulating (ia) neurons for exogenously applied da and 5,6-dihydroxytryptamine began to occur simultaneously on the hatching day (stage 25/26). endogenous da neurons were first detected on the third postnatal day (stage 26/27). the appearance of da and ia neurons was found to be ...19836652502
lack of orthogonal particle assemblies and presence of tight junctions in astrocytes of the goldfish (carassius auratus). a freeze-fracture study.the cytoplasmic membranes of astrocytes in the optic nerve of the goldfish were investigated by means of freeze-fracture techniques. astrocytes of normal and regenerating optic nerves did not differ in the fine structure of plasma membranes. emphasis is placed on the following results: astrocytic membranes of fish do not reveal the orthogonal particle assemblies that are believed to be generally characteristic for astrocytes in the white matter. astrocytes reveal extensive membrane areas occupie ...19836640627
effects of monosodium l-glutamate on pituitary innervation in goldfish, carassius auratus.monosodium l-glutamate was injected intraperitoneally into goldfish at a dosage of 2.5 mg/g body wt. the first noticeable effect in the pituitary, 30 min postinjection, was marked swelling of pituicytes in the neurohypophysis (nh). eighteen hours postinjection a large number of degenerating type b fibers were found in the nh of the proximal pars distalis (ppd), while all type a fibers, and type b fibers in other parts of the pituitary, remained unaffected. because glutamate causes a lesion in th ...19836628963
renal ammonia excretion and production in goldfish, carassius auratus, at low environmental ph.renal ammonia excretion and production were investigated in normal and acidotic goldfish (carassius auratus). goldfish were made acidotic by exposure to a low ph environment (ph 4.0), which caused a lowering of blood ph from 7.88 to 7.61. environmental acidification resulted in an increase in total renal ammonia excretion (from 0.43 to 1.00 mueq x 100 g-1 x h-1) and urinary concentration (from 0.58 to 0.76 mm), as well as a rise in renal titratable acid output (from -0.57 to +0.62 mueq x 100 g-1 ...19836624954
effects of hypothalamic lesions on serum growth hormone levels and growth rates in goldfish, carassius auratus.serum growth hormone (gh) levels and growth rates of goldfish were measured concurrently following discrete electrothermic lesions of a number of hypothalamic nuclei. following lesions of the nucleus preopticus periventricularis (npp), significant increases in serum gh levels were evident at 4 weeks postlesioning in comparison to both sham-operated and normal control groups. in addition, the npp-lesioned fish showed significant increases in both total body weight and standard length increments c ...19836618153
comparison of the radiosensitivity of three goldfish cell lines using short term endpoints.the induction and rejoining of dna strand breaks after gamma-irradiation in three goldfish (carassius auratus) cell lines with different sensitivities to the lethal effect of gamma-rays has been studied by the dna strand separation method using hydroxylapatite chromatography. the induction and rejoining of dna strand breaks was similar in all cell lines. there was also little difference in the degree of inhibition of dna synthesis immediately after irradiation. however, the rank orders of the du ...19846610658
involvement of the telencephalic hemispheres and the preoptic area in sexual behavior of the male goldfish, carassius auratus: a brain-lesion study.to locate the brain areas involved in sexual behavior, the effects on male sexual behavior of localized electrolytic lesions in various parts of the telencephalic hemispheres and the preoptic area and of gross ablations of parts of the telencephalic hemispheres were examined in goldfish. all three patterns of male sexual behavior examined (following, butting , and spawning) were severely impaired after bilateral lesions confined to the area ventralis telencephali pars supracommissuralis and/or p ...19846610412
nuclear transplantation in teleosts. hybrid fish from the nucleus of crucian and the cytoplasm of carp.hybrid fish are obtained from the combination of nucleus and cytoplasm from two genera of fresh-water teleosts using the technique of nuclear transplantation (i.e. the combination of the nucleus of crucian (carassius auratus) and the cytoplasm of carp (cyprinus carpio]. morphological characteristics of these hybrid fish that have been examined so far indicate that some features such as barbs, pharyngeal teeth, the number of scales along the lateral line, and the number of vertebrae are similar t ...19846597566
incidence of chromatoblastomas in aging goldfish (carassius auratus).the incidence of chromatoblastomas in aging goldfish, carassius auratus, was observed for 2 successive years. tumors first appeared in fish at 5 years of age, and the tumor frequency increased with increasing age of the fish. the cumulative incidence rate of chromatoblastomas and the mean number of tumors per fish were estimated for each age. the mean number of tumors per fish fitted a straight line with a slope of approximately 3 on a double logarithmic graph against age. thus tumor incidence s ...19836572741
in vitro estradiol-17 beta and testosterone production by ovarian follicles of the goldfish, carassius auratus.in vitro production of estradiol-17 beta and testosterone by ovarian follicles of goldfish (carassius auratus) at different developmental stages in response to human chorionic gonadotropin (hcg) was examined using 18-hr incubations. the vitellogenic follicles (primary and secondary yolk stage) produced estradiol-17 beta in response to hcg; follicles at the secondary yolk stage produced about five times more estradiol-17 beta than the primary yolk stage follicles. however, tertiary yolk stage fol ...19846539268
the effect of water acidification on prolactin cells and pars intermedia pas-positive cells in the teleost fish oreochromis (formerly sarotherodon) mossambicus and carassius auratus.although exposure to acid water (ph 3.5) induces severe and prolonged reduction in plasma osmolarity and total plasma calcium concentration in tilapia (oreochromis mossambicus) and goldfish (carassius auratus), the responses of the hypophyseal cells are clearly different. in tilapia, the size of the rostral pars distalis of the pituitary gland is enlarged as a result of the increase in size and number of prolactin cells. the pars intermedia pas-positive (pipas) cells are not noticeably changed. ...19846525622
effects of olfactory bulb ablation on cardiac and ventilatory arousal responses and their habituation in the goldfish carassius auratus.the effects of olfactory bulb ablation on the cardiac and ventilatory arousal responses to the onset of illumination and habituation of these responses were investigated in goldfish. bulbectomy did not effect the magnitude of response on novel stimulus presentation. normal, sham operated, and olfactory bulb-ablated animals showed similar rates of habituation. these results suggest that deficits in feeding and sexual behavior in goldfish are due to the loss of specific olfactory influences on low ...19846525140
temperature dependent attraction by goldfish to a chemical feeding cue presented alone and in combination with heated water.an eight cell multiple-choice experiment was used to investigate the influence of temperature on the locomotor response of goldfish (carassius auratus) to a chemical feeding cue. when a food extract was infused into a single cell at ambient temperatures of 26, 28, 30, 32, and 34 degrees c (ambient temperature=acclimation temperature) the goldfish spent more time in the extract cell than three similarly outfitted dummy cells at all temperatures, but the intensity of their attraction varied over t ...19846522471
[polymorphism of trypanosoma gruby, 1841 found in the blood of carp (carassius auratus gibelio (bloch), perch (perca fluviatilis l.) and stone perch (acerina cernua (l.))]. 19846516399
trypanosoma danilewskyi: host specificity and host's effect on morphometrics.carassius auratus, barbus conhus, danio malabaricus, catostomus commersoni, notropis cornutus, etheostoma caeruleum, and ictalurus nebulosus were susceptible to trypanosoma danilewskyi by intraperitoneal inoculation. trypanosomes isolated from all species of susceptible fishes were infective to goldfish. no trypanosomes were detected in inoculated semotilus atromaculatus, ambloplites rupestris, lepomis gibbosus and perca flavescens. fifty specimens were measured from each of 3 c. auratus, 2 b. c ...19846512642
structural and functional analysis of spontaneous anti-nitrophenyl antibodies in three cyprinid fish species: carp (cyrinus carpio), goldfish (carassius auratus) and tench (tinca tinca).high spontaneous anti-trinitrophenyl (tnp) activities were found in three cyprinid fish species: carp (cyprinus carpio), goldfish (carassius auratus) and tench (tinca tinca). the molecules involved, isolated by affinity chromatography on dinitrophenyl-lysine sepharose (dnp-lysine-sepharose), had the main characteristics of a high molecular weight immunoglobulin (igm-like). affinity measurements were performed on natural anti-dnp/tnp antibodies isolated from nine individual tench sera, using the ...19846500139
glial fibrillary acidic protein in regenerating teleost spinal cord.immunohistological and ultrastructural studies were carried out on normal and regenerating spinal cord of the gymnotid sternarchus albifrons, and in the brain and spinal cord of the goldfish carassius auratus, to examine the distribution of glial fibrillary acidic protein (gfap) in these tissues. sections of normal goldfish brain and spinal cord exhibited positive staining for gfap. in normal sternarchus spinal cord, electron microscopy has revealed filament-filled astrocytic processes; however, ...19846481149
performance of goldfish (carassius auratus) in patterned sequences of rewarded and nonrewarded trials.the performance of goldfish was studied in a series of experiments with patterned sequences of reward (r) and nonreward (n) for response to a given stimulus (s3). trials with two other, readily discriminable stimuli, response to one always rewarded and response to the second never rewarded, were interpolated in such a way that the outcome of any s3 trial could not be predicted from the events of the immediately preceding trial. the main purpose was to look for control of response to s3 based on ...19846478787
gonadotropin release from the pars distalis of goldfish, carassius auratus, transplanted beside the brain or into the brain ventricles: additional evidence for gonadotropin-release-inhibitory factor.the pars distalis of the pituitary was transplanted from one goldfish to another, of matched gonadal condition and body size, either beside the brain ("juxta" location) or into the brain ventricle in the preoptic region or under the optic tectum. recipients of a pars distalis had significantly higher serum gonadotropin (gth) levels than sham-operated controls without transplants; following removal of the transplants, serum gth levels decreased to levels found in controls within 24 hr. this demon ...19846468914
light- and electron-microscopic demonstration of immunoreactive opsin in the pinealocytes of various vertebrates.an antibody to opsin isolated from rod outer segments of the frog retina was applied in light- and electron-microscopic immunocytochemical studies to the pineal organ of various vertebrates (cyprinus carpio, carassius auratus, rana esculenta, emys orbicularis, pseudemys scripta elegans, lacerta agilis and viridis. gallus domesticus, columba livia, melopsittacus undulatus, serinus canaria, taeniopyga punctata, didelphis virginiana, erinaceus roumanicus, myotis myotis, rabbit, rat, cat).19816458362
[experimental study of the bioaccumulation of inorganic mercury and methylmercury in the goldfish (carassius auratus l.)].the study of the mercury and methylmercury bioaccumulation by various species of fish is only possible experimentally when the animals are maintained fasting during a few days. with a such experimentation in the goldfish (carassius auratus l.), the authors are showing that methylmercury is much more accumulated than inorganic mercury: the transfert factor is about 760 to 780 for the methylmercury chloride and only 90 for the mercuric chloride. in fact a lot of variables occur in this phenomena o ...19806449276
toxicity of carbaryl and 1-naphthol to goldfish (carassius auratus) and killifish (fundulus heteroclitus). 19836416338
lethal and mutagenic effects of radiation and chemicals on cultured fish cells derived from the erythrophoroma of goldfish (carassius auratus).gem 199 cells derived from an erythrophoroma of goldfish (carassius auratus), which had a high plating efficiency, were used to investigate the lethal and mutational effects of radiations (uv and gamma-rays) and chemicals (4nqo and mnng). the cells were more resistant to gamma-rays than mammalian cells and caf-mm1 cells derived from the normal fin tissue of goldfish. they were also more resistant to uv-irradiation than caf-mm1 cells. photoreactivation after uv-irradiation was present in gem 199 ...19836408467
the release of gamma-aminobutyric acid from horizontal cells of the goldfish (carassius auratus) retina.isolated horizontal cells from goldfish retinas were prepared by enzymatic dissociation using papain and separated from other cells by velocity sedimentation. in the intact retina, h1 horizontal cells possess a high-affinity mechanism for accumulating gamma-aminobutyric acid (gaba). this property is retained in isolated cells, which also release the accumulated gaba in response to depolarization by elevated external k+. l-glutamic acid and its analogues are highly effective at micromolar concent ...19846387085
immunocytochemical localization of luteinizing hormone-releasing hormone in the brain of the goldfish carassius auratus.using an indirect immunofluorescence technique, lh-rh was localized in the brain of the goldfish (carassius auratus). immunoreactive cell bodies were inconsistantly found in two hypothalamic nuclei: the anteroventral nucleus preopticus periventricularis (npp) and the posterior nucleus lateralis tuberis (nltp). numerous immunoreactive fibers were observed in hypothalamic as well as in the extrahypothalamic parts of the brain and in the proximal pars distalis of the pituitary gland. our results ar ...19846370786
beta 2 microglobulin-like polypeptide of the goldfish, carassius auratus.antisera to human beta 2 microglobulin (beta 2m) detected a plasma membrane molecule on goldfish (carassius auratus) cells in immunofluorescence. a goldfish molecule detected by radioimmunoassay (ria) co-eluted with human beta 2m on gel filtration. by affinity chromatography on immobilized antibody to human beta 2m, a molecule was purified (from extracts of goldfish) that showed, on sds-polyacrylamide gel electrophoresis, a mobility similar to that of human beta 2m (apparent mr 12,800 +/- 500).19846369118
serotonin in the brain of the goldfish, carassius auratus. an immunocytochemical study.the distribution of immunoreactive serotonin (5-ht) was investigated in the brain and pituitary gland of the goldfish with the use of indirect immunofluorescence technique. immunoreactive cerebrospinal fluid-contacting neurons were found in the nucleus recessus lateralis and in the nucleus recessus posterioris of the hypothalamus. additional immunoreactive neurons were observed ventro-lateral to the posterior commissure in the nucleus dorsolateralis thalami. this group of cell bodies extends pos ...19836357477
effects of pimozide and des gly10,[d-ala6]luteinizing hormone-releasing hormone ethylamide on serum gonadotropin concentrations, germinal vesicle migration, and ovulation in female goldfish, carassius auratus.in gravid female goldfish held at 10-12 degrees, a single injection of des gly10,[d-ala6]luteinizing hormone-releasing hormone ethylamide (lhrh-a), or 2 injections of lhrh-a given 12 hr apart, increased serum gonadotropin (gth) concentrations, but did not stimulate germinal vesicle migration or ovulation. injection of a dopamine antagonist, pimozide (pim), increased serum gth levels. pim injected with the second of two lhrh-a injections not only potentiated the lhrh-a-induced increase in serum g ...19836354840
effects of dopamine on gonadotropin release in female goldfish, carassius auratus.intraperitoneal injections of dopamine (da) or its agonist, apomorphine decreased and pimozide, a da antagonist, increased serum gonadotropin (gth) levels in normal female goldfish. injection of da into the third cranial ventricular did not alter serum gth concentrations. intraperitoneal injections of da or apomorphine each reduced the highly elevated serum gth levels caused by preoptic lesions which abolish an inhibitory hypothalamic influence on gth release allowing prolonged spontaneous relea ...19836343907
effects of cadmium-enriched sediment on fish and amphibian embryo-larval stages.aquatic toxicity tests were conducted to evaluate the effects of cadmium-enriched sediment on embryo-larval stages of the goldfish (carassius auratus), leopard frog (rana pipiens), and largemouth bass (micropterus salmoides). natural stream sediment was collected and enriched with cadmium to nominal concentrations of 1.0, 10.0, 100, and 1000 mg/kg. enriched sediments were placed in pyrex dishes and covered with 350 ml of reconstituted water. fertilized eggs were placed in the dishes and maintain ...19846332728
influence of catecholamines on gonadotropin secretion in goldfish, carassius auratus.serum gonadotropin (gth) concentrations in female goldfish were measured before and at various times after intraperitoneal injection of drugs altering catecholamine synthesis and neural activities. reserpine, a depleter of neurotransmitter stores, elevated serum gth levels compared to controls, suggesting the general involvement of neurotransmitters in altering gth release. 6-hydroxydopamine, a catecholaminergic neurotoxin, increased serum gth concentration, suggesting that catecholaminergic neu ...19836298058
isolation of xanthophores from the goldfish (carassius auratus l.).a method is described for the isolation of milligram quantities of viable, hormone-responsive xanthophores from goldfish scales. the preparations are typically 70 to 90% pure and are useful for biochemical analyses or for establishing primary cultures.19826288547
histological and enzyme histochemical studies on the nephrons of the freshwater fishes, cyprinus carpio and carassius auratus.the nephrons of carp (cyprinus carpio) and goldfish (carassius auratus) were examined histologically and also histochemically for enzymes. in both species the distal and collecting tubules have much wider lumens than do the other renal tubules; thus urine probably flows more slowly in these larger tubules. enzyme histochemistry shows that epithelium of the neck and proximal and intermediate tubules respires anaerobically, whereas that of the distal and collecting tubules respires aerobically. th ...19826286982
characterization of and hormonal effects on subcellular fractions from xanthophores of the goldfish carassius auratus l. 19816272788
effects of lithium chloride on lateral line responses in carassius auratus. 19806253837
distribution of neurofilaments in myelinated axons of the optic nerve of goldfish (carassius auratus l.).neurofilaments were counted in myelinated axons of the optic nerve of goldfish which were acclimated to 5 degrees and 25 degrees c. the number of neurofilaments increases markedly with increasing axonal size; axons of less than 0.1 micrometer 2 in area contain between 25 and 60 neurofilaments, while in the larger axons of area greater than 1.0 micrometer 2 there are approximately 190. the densities of the neurofilaments in the small axons are noticeably higher than in the larger ones (507 and 16 ...19806253602
[phosphatases and oxidative enzymes in the kidney of the prussian carp (carassius auratus gibelio bloch) adapted to salt water].the distribution and activities of phosphatases and oxidative enzymes have been determined with the help of histochemical methods in the kidney of the prussian carp, a stenohaline freshwater-fish. in addition to fish maintained in freshwater aquaria, a group of the animals used has been adapted to seawater of moderate salinity. the following pattern of enzyme reaction intensities has been observed in the various kidney structures: strong reactions of alkaline phosphatase in the nephron are confi ...19806251647
cerebrospinal fluid-contacting neurons, sensory pinealocytes and landolt's clubs of the retina as revealed by means of an electron-microscopic immunoreaction against opsin.opsin-immunoreactive sites of hypothalamic cerebrospinal fluid (csf)-contacting neurons, pinealocytes and retinal cells were studied in various vertebrates (carassius auratus, phoxinus phoxinus, triturus cristatus, bombina bombina, rana esculenta) by means of postembedding immuno-electron microscopy with the use of the protein a-gold labeling method. the retina of the rat served as a general reference tissue for the quality of the immunocytochemical reaction. a strong opsin immunoreaction (rat-a ...19836226359
histochemical analysis of muscle fibers in myotome of teleost fishes (carassius auratus gibelio).qualitative histochemical studies were carried out on lateral musculature of carassius auratus gibelio. on the basis of some metabolic enzymes (sdh, ldh) and myofibrillar atp-ase activities, red, intermediate, white and small diameter muscle fibers were discerned. when sdh method was used, two other fiber types were distinguished. they built up a transition zone, which lay between intermediate and white muscle fiber complexes. the histochemical reaction for myofibrillar atp-ase activity, after a ...19826219051
immunoreactive opsin in the pineal organ of reptiles and birds.the presence of opsin was investigated with light microscopic immunocytochemistry in pinealocytes of reptiles and birds (emys orbicularis, pseudemys scripta elegans, lacerta agilis et viridis, gallus domesticus, columba livia, melopsittacus undulatus, serinus canaria, taeniopyga punctate). the outer segments of pinealocytes selectively bound antiopsin antibody as revealed by indirect immunocytochemical techniques, indicating the occurrence of a rhodopsin-like photopigment in these structures. th ...19826213109
effect of ascorbic acid on biotransformation and modification of the toxicity of mercurials in goldfish (carassius auratus). 19826212265
three distinct morphological classes of receptors in fish olfactory organs.three morphologically distinct classes of receptor-neurons are proposed: (1) type i ciliar cells, (2) microvillar cells and (3) type ii ciliar cells. retrograde transport of horseradish peroxidase by axons in the olfactory nerve to the olfactory organs of goldfish (carassius auratus) and channel catfish (ictalurus punctatus) provided evidence that these axon-bearing cells are present in the organs of both species. goldfish olfactory organs were also studied with scanning electron microscopy, dis ...19846199384
origin of efferent fibers of the vestibular apparatus in goldfish. a horseradish peroxidase study.after injecting a horseradish peroxidase (hrp) solution into the vestibular apparatus in goldfish (carassius auratus), cells in the medulla oblongata exhibited retrogradely transported hrp reaction product. these labeled cells were confined to 3 nuclei, i.e. the nucleus motorius tegmenti of bartelmez (with the most prominent labeled cell groups), the ventral vestibular nucleus (dieter's nucleus) and the medial vestibular nucleus. we consider these labeled cells to be the origin of the efferent i ...19806189014
characterization of benzo[a]pyrene metabolites formed by 3-methylcholanthrene-induced goldfish, black bullhead and brown bullhead.metabolites of bap formed by 3-mc-induced goldfish (carassius auratus), black bullhead (ictalurus melas), brown bullhead (ictalurus nebulus) and rat have been separated by hplc. the main metabolites in both bullhead species and the rat were 3-hydroxy-bap and bap 9,10- and 7,8-dihydrodiols, whereas bap 1,6- and 3,6-diones and 9- and 3-hydroxy-bap predominated in the goldfish. induced rat formed over 10 times as many total metabolites as the fish. no induction was observed in the 3-mc-treated brow ...19846149861
the in vitro metabolism by homogenates of goldfish (carassius auratus) liver of pentachloro-2-(chloromethyl sulphonamido) diphenyl ether, the major component and active ingredient of the mothproofing agent eulan wa new.after dosing goldfish (carassius auratus) for 2 hr with pentachloro-2-(chloromethyl sulphonamido) diphenyl ether, 6-pcsd, (the major component of the active ingredients of the mothproofing agent eulan wa new) in the surrounding water, high concentrations of the corresponding amine pentachloro-2-aminodiphenyl ether, 5-pad, were found in the liver. this suggests that the liver is a primary site of 5-pad formation in vivo. homogenates of goldfish liver showed a 5-pad forming activity when incubated ...19846146475
the effects of somatostatin on serum growth hormone levels in the goldfish, carassius auratus.in the present study the effects of somatostatin on serum growth hormone (gh) levels in the goldfish, carassius auratus, were investigated. a single intraperitoneal injection of either 0.1 or 1.0 micrograms somatostatin/g body wt caused a significant decrease in serum gh levels at 1 h postinjection compared to vehicle-injected controls. two intraperitoneal injections of somatostatin (1.0 micrograms/g body wt), given 12 hr apart, caused a significant decrease in serum gh levels, compared to both ...19846144614
effects of clomiphene citrate, a nonsteroidal antiestrogen, on brain monoaminergic mechanisms in female goldfish, carassius auratus.the antiestrogen, clomiphene citrate ( merrell -national laboratories) was administered to female goldfish in order to test the hypothesis that this drug may act on brain monoaminergic mechanisms. brain monoamine oxidase (mao) activity and hypothalamic serotonin (5-ht) content were measured fluorometrically after i.p. administration of 0, 5 or 25 micrograms clomiphene citrate/g body wt. brain mao activity was significantly inhibited by the high dose of clomiphene citrate, whereas hypothalamic 5- ...19846144439
responses of solitary retinal horizontal cells from carassius auratus to l-glutamate and related amino acids.effects of l-glutamate and its analogues on membrane potentials of solitary horizontal cells were studied by intracellular recording. l-glutamate depolarized these cells at micromolar concentrations (greater than or equal to 10 microm), while d-glutamate and l-alpha-amino adipic acid produced slight depolarizations only at millimolar concentrations. neither l- nor d-aspartate, even at millimolar doses, produced any change in solitary horizontal-cell resting potential. solitary horizontal-cell re ...19846143822
carbon dioxide production by red skeletal muscle of goldfish (carassius auratus l.) aerobic and anaerobic metabolism of glucose and glutamate.oxygen uptake and metabolic co2 production by lateral red muscle of goldfish have been measured in vitro. added glucose 6-phosphate depresses the rate of oxygen uptake by minced red muscle (crabtree effect). total co2 production is stimulated resulting in a respiratory quotient which is considerably greater than one. 14co2 release from [u-14c] glucose 6-phosphate and [u-14c] glutamate continues during anoxia. no activity of the hexose monophosphate shunt was observed. the results suggest that bo ...19846141024
drug-oxidizing mono-oxygenase system in liver microsomes of goldfish (carassius auratus).the fractionation of the liver of goldfish (carassius auratus) was studied, and the properties of the microsomal fraction were examined. the microsomal fraction contained cytochrome p-450 and catalyzed the oxidation of aminopyrine, aniline, 7-ethoxycoumarin and benzo(a)pyrene. the oxidation activities were significantly lower than those of rat liver microsomes. the titration of cytochrome p-450 by potassium cyanide indicated the presence of multiple forms of cytochrome p-450 in goldfish liver mi ...19836139256
optic tectum adenylate cyclase activity from goldfish (carassius auratus) as a model to assess dopamine receptor agonists and antagonists.the distribution of adenylate cyclase activity in several discrete areas of brain in the goldfish (carassius auratus) and the responsiveness of this activity in the optic tectum to various neurotransmitters were studied. adenylate cyclase activity was found to have an uneven distribution in the brain, the highest concentrations occurring in the telencephalon. a dopamine-sensitive adenylate cyclase was found in the optic tectum. the increase in camp formation induced by dopamine was selectively p ...19836133329
altered activities of branchial and renal na/k- and mg-atpases in cold-acclimated goldfish (carassius auratus).1. cold-acclimated (1 degree c) goldfish (carassius auratus) branchial na/k-atpase activity was elevated 100% while renal na/k-atpase activity was not significantly affected compared with warm-acclimated (20 degrees c) goldfish. 2. cold-acclimated goldfish branchial and renal mg-atpase activity was reduced 18 and 30% on a per mg protein basis, respectively. 3. renal na/k-atpase activity was 4.6- and 1.6-fold greater than gill in cold- and warm-acclimated fish, respectively. 4. the elevated branc ...19836132750
effects of a dietary deficiency in calcium on growth and calcium uptake from the aquatic environment in the goldfish, carassius auratus.1. young goldfish, carassius auratus, were fed a calcium-deficient diet for 8 or 56 days and then their net uptake of calcium from the aquatic environment was examined using 45ca. 2. they showed normal growth and the normal level of plasma calcium in both experiments. 3. in the short-term experiment, no dietary effect was found in the uptake of environmental calcium. in the long-term experiment, however, the rate of uptake was significantly stimulated by 18%. 4. the results indicate the presence ...19836131769
similar antagonism of morphine analgesia by mif-1 and naloxone in carassius auratus.prolyl-leucyl-glycinamide (mif-1), the c-terminal tripeptide of oxytocin, and naloxone were administered intracranially (ic) to goldfish (carassius auratus) in doses of 0.001, 0.01, 0.1, 1.0 and 10.0 mg/kg and compared to a diluent control group for their ability to reduce the effects of morphine (30 mg/kg ic) in an assay measuring analgesia to electric shock. threshold levels of pain were determined by the voltage necessary to produce an agitated swimming response (asr). both mif-1 and naloxone ...19826129644
in vivo and in vitro effects of temperature on monoamine oxidase activity in brain and other tissues of the goldfish. carassius auratus l.1. the maximum velocity (vmax) and apparent michaelis constant (km) of brain and liver monoamine oxidase (mao) in goldfish were different in fish acclimated to 22 degrees c and to 7 degrees c ambient temperature. 2. in brain, vmax and km were dependent upon incubation temperature, but both parameters were lower in 7 degrees c, adapted fish over most of the incubation temperature range. 3. the values obtained for km showed a plateau at incubation temperatures at and below 25 degrees c for warm wa ...19826128171
[immunocytochemical distribution of somatostatin in the forebrain of two teleosts, the goldfish (carassius auratus) and gambusia sp (author's transl)].using immunofluorescence somatostatin was localized in the forebrain of the goldfish and gambusia. immunoreactive cell bodies were detected in the nucleus preopticus periventricularis (npp), the nucleus lateralis tuberis (nlt) and a dorsomedial nucleus at the level of the posterior nucleus anterioris periventricularis (napv) and of the rostral part of the nucleus ventromedialis thalami (nvm). in the pituitary, immunoreactive terminals were restricted at the proximal pars distalis.19826124310
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