Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| cell growth: the power of symplastic isolation. | cotton (gossypium hirsutum) fibers are single-celled seed coat hairs that elongate up to 2mm per day during a phase of rapid growth. recent evidence suggests this growth is orchestrated by a series of events which includes temporary closure of plasmodesmata. | 2001 | 11516666 |
| cloning of peroxisomal ascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in arabidopsis thaliana. | a full-length cdna clone (hvapx1) encoding a peroxisomal type ascorbate peroxidase was isolated from barley (hordeum vulgare cv. haruna-nijyo) leaves by differential display. the deduced amino acid sequence of the hvapx1 gene had 75.3% homology to that from the gossypium hirsutum glyoxysomal apx gene and 72.1% homology to that from the arabidopsis thaliana peroxisomal apx gene, apx3. southern blot analysis indicated that a single-copy gene in the barley genome encoded hvapx1. northern blot analy ... | 2001 | 11483357 |
| a putative plant homolog of the yeast beta-1,3-glucan synthase subunit fks1 from cotton (gossypium hirsutum l.) fibers. | a novel plant gene cfl1 was cloned from cotton (gossypium hirsutum l.) fibers by expressed sequence tag (est) database searching and 5'-race (rapid amplification of cdna ends). this gene shows sequence homology with fks1 which has been identified as the putative catalytic subunit of the yeast beta-1,3-glucan synthase. it encodes a protein (cfl1p) of 219 kda with 13 deduced transmembrane helices and 2 large hydrophilic domains, one of which is at the n-terminus and the other in the internal regio ... | 2001 | 11469587 |
| the experimental herbicide cga 325'615 inhibits synthesis of crystalline cellulose and causes accumulation of non-crystalline beta-1,4-glucan associated with cesa protein. | developing cotton (gossypium hirsutum) fibers, cultured in vitro with their associated ovules, were used to compare the effects of two herbicides that inhibit cellulose synthesis: 2,6-dichlorobenzonitrile (dcb) and an experimental thiatriazine-based herbicide, cga 325'615. cga 325'615 in nanomolar concentrations or dcb in micromolar concentrations causes inhibition of synthesis of crystalline cellulose. unlike dcb, cga 325'615 also causes concomitant accumulation of non-crystalline beta-1,4-gluc ... | 2001 | 11457949 |
| impact of bt cottons expressing one or two insecticidal proteins of bacillus thuringiensis berliner on growth and survival of noctuid (lepidoptera) larvae. | a series of laboratory assays were performed to compare the relative impact of commercial and experimental cultivars of cotton, gossypium hirsutum (l.), expressing zero, one, or two insecticidal proteins of bacillus thuringiensis berliner, on several lepidopteran pests. assays in which larvae were fed fresh plant tissue indicated that dual-toxin b. thuringiensis (bt) cultivars, expressing both cry1ac and cry2ab endotoxins of b. thuringiensis, were more toxic to bollworms, helicoverpa zea (boddie ... | 2001 | 11425033 |
| fate and toxicity of endosulfan in namoi river water and bottom sediment. | endosulfan (6,7,8,9,10,10,-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-oxide) sorption (standardized to 1% total organic carbon and dry weight) was significantly (p < 0.05) more concentrated on the large (>63 microm) particle fraction compared with smaller size fractions (<5 microm and 5-24 microm) of bottom sediments from the namoi river, australia. following completion of the particle size fractionation (6 to 12 wk) and a sediment toxicity assessment (2 wk), the s ... | 2013 | 11401264 |
| endosulfan transport: i. integrative assessment of airborne and waterborne pathways. | to reduce endosulfan (c9h6o3cl6s; 6,7,8,9,10,10-hexachloro-1,5, 5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide) contamination in rivers and waterways, it is important to know the relative significances of airborne transport pathways (including spray drift, vapor transport, and dust transport) and waterborne transport pathways (including overland and stream runoff). this work uses an integrated modeling approach to assess the absolute and relative contributions of these pathways ... | 2013 | 11401261 |
| simulating endosulfan transport in runoff from cotton fields in australia with the gleams model. | endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,4,3-benzodioxathiepin 3-oxide), a pesticide that is highly toxic to aquatic organisms, is widely used in the cotton (gossypium hirsutum l.) industry in australia and is a risk to the downstream riverine environment. we used the gleams model to evaluate the effectiveness of a range of management scenarios aimed at minimizing endosulfan transport in runoff at the field scale. the field management scenarios simulated were (i) ... | 2013 | 11401260 |
| spray drift of pesticides arising from aerial application in cotton. | this paper presents results from field studies carried out during the 1993-1998 australian cotton (gossypium hirsutum l.) seasons to monitor off-target droplet movement of endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide) insecticide applied to a commercial cotton crop. averaged over a wide range of conditions, off-target deposition 500 m downwind of the field boundary was approximately 2% of the field-applied rate with oil-based applicatio ... | 2013 | 11401259 |
| off-site movement of endosulfan from irrigated cotton in new south wales. | the fate and transport of endosulfan (6,7,8,9,10,10-hexachloro-1,5, 5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin 3-oxide) applied to cotton (gossypium hirsutum l.) fields were studied throughout three consecutive years on two selected locations in new south wales (australia). rates of dissipation from foliage and soil, volatilization from the field, and transport of residues in irrigation and/or storm runoff waters were measured in order to estimate a total field balance. dissipation ... | 2013 | 11401258 |
| growth of cotton plants (gossypium hirsutum) as affected by water and sludge from a sewage treatment plant: ii. seed and fiber yield and heavy metal accumulation. | 2001 | 11353376 | |
| growth of cotton plants (gossypium hirsutum) as affected by water and sludge from a sewage treatment plant: i. plant phenology and development. | 2001 | 11353375 | |
| comparative development of fiber in wild and cultivated cotton. | one of the most striking examples of plant hairs is the single-celled epidermal seed trichome of cultivated cotton. the developmental morphology of these commercial "fibers" has been well-characterized in gossypium hirsutum, but little is known about the pattern and tempo of fiber development in wild gossypium species, all of which have short, agronomically inferior fiber. to identify developmental differences that account for variation in fiber length, and to place these differences in a phylog ... | 2010 | 11256432 |
| effect of heat stress on the inhibition and recovery of the ribulose-1,5-bisphosphate carboxylase/oxygenase activation state. | experiments were conducted to determine the relative contributions of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco; ec 4.1.1.39) activation state vis-a-vis rubisco activase and metabolite levels to the inhibition of cotton (gossypium hirsutum l.) photosynthesis by heat stress. exposure of leaf tissue in the light to temperatures of 40 or 45 degrees c decreased the activation state of rubisco to levels that were 65 or 10%, respectively, of the 28 degrees c control. ribulose-1,5-bispho ... | 2000 | 11219585 |
| effect of light intensity on in vitro multiple shoot induction and regeneration of cotton (gossypium hirsutum l. cv khandawa-2). | cotyledonary nodes taken alongwith shoot apex from seedlings of cotton (g. hirsutum) proliferated into shoots on nutrient agar medium supplemented with cytokinins. in the presence of optimal plant growth regulators, low light intensity enhanced the number of shoots initiated per explant in cotton. an average of 33.5 +/- 2.9 shoots were obtained from a single explant cultured for 8 weeks which is about four fold higher than the values reported in earlier protocols. the isolated shoots were rooted ... | 2000 | 11218821 |
| changes in molecular species of triacylglycerols in developing cotton seeds under salt stress. | cotton plants (gossypium hirsutum l. cv. s4) were grown and irrigated with increasing salt concentrations: 0, 50, 100 and 150 mm nacl. lipids from developing seeds, at stages of 3, 4, 5 and 6 weeks after anthesis, were analysed. high salt dose (150 mm nacl) affected triacylglycerol levels drastically at the end of ripening. hplc analysis of triacylglycerols in control seeds showed ten molecular species. palmitodilinolein (pll) is the major fraction, representing about 25% of total triacylglycero ... | 2000 | 11171251 |
| genetic diversity evaluation of some chinese elite cotton varieties with rapd markers. | rapds analysis was used to assess the genetic diversity of some elite cotton varieties. 17 cotton varieties or breeding lines were screened with 200 arbitrary primers to generate 113 polymorphic bands. cluster analysis and upgma showed that 17 varieties could be put into 3 clusters corresponding to genome difference. the genetic similarity among them ranged from 0.2000 to 0.8451. three elite varieties ejing-1, sumian-3 and zhongmiansuo-12 are highly similar in genetic base. 7581, an offspring fr ... | 2000 | 11132498 |
| [genetic analysis on flowering and boll setting in upland cotton (gossypium hirsutum l.). iii. genetic behavior at different developing stages]. | an additive-dominant genetic model with genotype by environment interaction was employed to analyze the genetic behavior of flowering and fruiting at different development stages, with 2-year data from 4 x 4 diallel crosses. the results of variance analysis indicated that flowering and fruiting behaviors were mainly controlled by dominant effects at early period, but by additive effects later on. compared with genetic main effects, ge interaction effects playeda minor role on the flowering and f ... | 2000 | 11132496 |
| electron transport through photosystem ii in leaves during light pulses: acceptor resistance increases with nonphotochemical excitation quenching. | light response curves of photosystem (ps) ii electron transport from oxygen evolving complex to plastoquinone (pq) were measured in sunflower (helianthus annuus l.), cotton (gossypium hirsutum l.) and tobacco (nicotiana tabacum l.) leaves by recording o(2) evolution and fluorescence in 5-200 ms light pulses of 500-13500 micromol absorbed quanta m(-2) s(-1). the leaves were pre-adapted at 60-2000 micromol quanta m(-2) s(-1) for 60 min to obtain different nonphotochemical excitation quenching, whi ... | 2000 | 11106767 |
| 2,4-dichlorophenoxyacetic acid metabolism in transgenic tolerant cotton (gossypium hirsutum). | the metabolic fate of 2,4-dichlorophenoxyacetic acid (2,4-d) was studied in leaves of transgenic 2,4-d-tolerant cotton (gossypium hirsutum), which is obtained by transfer of the tfda gene from the bacterium alcaligenes eutrophus. the tdfa gene codes for a dioxygenase catalyzing the degradation of 2,4-d to 2, 4-dichlorophenol (2,4-dcp). [phenyl-(14)c]-2,4-d was administered by petiolar absorption followed by an 18 h water chase or converted to the isopropyl ester and sprayed onto the leaf surface ... | 2000 | 11087477 |
| thermal dependence of malate synthase activity and its relationship to the thermal dependence of seedling emergence. | cotton yields are often reduced by low temperatures at early planting dates. improved seedling metabolism at low temperatures may enhance seedling performance. the glyoxylate cycle plays a role in the metabolism of stored lipids, and thus thermal limitations on the function of malate synthase (ec 4.1.3.2) may be involved in low-temperature limitations on seedlings. the thermal dependencies of the apparent k(m) and maximal velocity for the malate synthases from cotton (gossypium hirsutum l.) and ... | 2000 | 11052697 |
| feeding behavior of bollworm and tobacco budworm (lepidoptera: noctuidae) larvae in mixed stands of nontransgenic and transgenic cotton expressing an insecticidal protein. | feeding behavior of third-instar bollworm, helicoverpa zea (boddie), and tobacco budworm, heliothis virescens (f.), was observed in pure and mixed stands of nontransgenic and transgenic cotton (btk), gossypium hirsutum l., expressing an insecticidal protein cryia(c) from a bacterium, bacillus thuringiensis berliner subsp. kurstaki. five plant stands composed of btk and non-btk plants were evaluated; two pure stands and three mixed stands. percentage ratios of btk to non-btk plants in the stands ... | 2000 | 10985046 |
| effects of mild night chilling on respiration of expanding cotton leaves. | utilizing expanding leaves of cotton (gossypium hirsutum l. cv. coker 312), the hypothesis that suboptimal night temperatures above those for putative phase transitions of mitochondrial lipids caused greater substrate control of night respiration and increased the control that respiration exerted on atp-dependent metabolism was tested. night respiratory co(2) evolution rates for 5-7-day-old leaves growing at 30/19 degrees (day/night) nearly equaled those of leaves exposed to 28 degrees c nights, ... | 2000 | 10960737 |
| multilocus interactions restrict gene introgression in interspecific populations of polyploid gossypium (cotton). | experimental advanced-generation backcross populations contain individuals with genomic compositions similar to those resulting from interspecific hybridization in nature. by applying a detailed restriction fragment length polymorphism (rflp) map to 3662 bc3f2 plants derived from 24 different bc1 individuals of a cross between gossypium hirsutum and g. barbadense, large and widespread deficiencies of donor (g. barbadense) chromatin were found, and seven independent chromosomal regions were entir ... | 2000 | 10937254 |
| cloning of a gene for an acyl-coa dehydrogenase from pisum sativum l. and purification and characterization of its product as an isovaleryl-coa dehydrogenase. | isovaleryl-coa dehydrogenase (ivd, ec ) catalyzes the third step in the catabolism of leucine in mammals. deficiency of this enzyme leads to the clinical disorder isovaleric acidemia. ivd has been purified and characterized from human and rat liver, and the x-ray crystallographic structure of purified recombinant human ivd has been reported. nothing is known about ivd activity in plants, although cdna clones from arabidopsis thaliana and partial sequences from gossypium hirsutum and oryza sativa ... | 2000 | 10913142 |
| ty1-copia-retrotransposon behavior in a polyploid cotton. | retrotransposons constitute a ubiquitous and dynamic component of plant genomes. intragenomic and intergenomic comparisons of related genomes offer potential insights into retrotransposon behavior and genomic effects. here, we have used fluorescent in-situ hybridization to determine the chromosomal distributions of a ty1-copia-like retrotransposon in the cotton ad-genome tetraploid gossypium hirsutum and closely related putative a- and d-genome diploid ancestors. retrotransposon clone a108 hybri ... | 2000 | 10730591 |
| dynamics of non-structural carbohydrates in developing leaves, bracts and floral buds of cotton. | development of cotton (gossypium hirsutum l.) squares (i.e. floral buds with bracts) is fundamental for yield formation. a 2-year field study was conducted to determine dry weight (dw) accumulations of cotton leaves, floral bracts and floral buds, and the changes in concentrations of non-structural carbohydrates (hexoses, sucrose and starch) in these tissues during square ontogeny as affected by fruiting positions within the plant canopy. during square development, dw accumulation of a subtendin ... | 2000 | 10725518 |
| salicylic acid mediated by the oxidative burst is a key molecule in local and systemic responses of cotton challenged by an avirulent race of xanthomonas campestris pv malvacearum. | we analyzed the production of reactive oxygen species, the accumulation of salicylic acid (sa), and peroxidase activity during the incompatible interaction between cotyledons of the cotton (gossypium hirsutum) cv reba b50/xanthomonas campestris pv malvacearum (xcm) race 18. sa was detected in petioles of cotyledons 6 h after infection and 24 h post inoculation in cotyledons and untreated leaves. the first peak of sa occurred 3 h after generation of superoxide (o(2)(.-)), and was inhibited by inf ... | 2000 | 10712539 |
| identification and expression of cotton (gossypium hirsutum l.) plastidial carbonic anhydrase. | four carbonic anhydrase (ca) cdna clones were isolated from a 48 h dark-grown cotton (gossypium hirsutum l.) seedling cdna library. nucleotide sequence analysis revealed two different ca isoforms designated ghca1 and ghca2. the encoded polypeptides possess n-terminal serine/threonine-rich regions indicative of plastid transit peptides, and approximately 80% sequence identity to other plant plastidial beta-cas. the ghca1 cdna encodes a nearly complete preprotein of 323 amino acids with a molecula ... | 1999 | 10682348 |
| an assessment of alphaalpha-tubulin isotype modification in developing cotton fiber. | multiple isoforms of alpha- and beta-tubulin accumulate in higher plant cells, including cotton (gossypium hirsutum l.) fiber. isotypes may originate either by transcription of distinct genes or by posttranslational modification of gene products. the existence of two types of posttranslational modification in cotton fiber alpha-tubulin has been examined by immunoblotting cotton fiber proteins from two developmental stages and by probing with specific monoclonal antibodies to acetylated (6-11b-1) ... | 2000 | 10648195 |
| expression of a lipid transfer protein gene family during cotton fibre development. | there are six to eight lipid transfer protein-related genes in the allotetraploid gossypium hirsutum genome, three of which, fsltp1, fsltp2 and fsltp3, were isolated in two genomic clones. all three genes potentially encode full-length lipid transfer proteins (ltps), but sequence differences between these and the cotton fibre-specific cdna previously reported, pfs6, suggest that none is likely to encode the fibre transcripts. gene-specific rt-pcr experiments showed that expression patterns of th ... | 2000 | 10634943 |
| identification and cloning of a complementary dna encoding a vicilin-like proprotein, jug r 2, from english walnut kernel (juglans regia), a major food allergen. | walnuts and other tree nuts are important food-allergen sources that have the potential to be associated with life-threatening, ige-mediated systemic reactions in some individuals. | 1999 | 10589017 |
| duplicated genes evolve independently after polyploid formation in cotton. | of the many processes that generate gene duplications, polyploidy is unique in that entire genomes are duplicated. this process has been important in the evolution of many eukaryotic groups, and it occurs with high frequency in plants. recent evidence suggests that polyploidization may be accompanied by rapid genomic changes, but the evolutionary fate of discrete loci recently doubled by polyploidy (homoeologues) has not been studied. here we use locus-specific isolation techniques with comparat ... | 1999 | 10588718 |
| mobility of the herbicide pyrithiobac through intact soil columns. | the relative mobility of pyrithiobac [sodium 2-chloro-6-(4, 6-dimethoxypyrimidin-2-ylthio)benzoate], a new herbicide used for postemergence control of broadleaf weeds in cotton (gossypium hirsutum), was evaluated and compared against that of bromide (br(-)) tracer on four soils representative of cotton-growing regions using intact soil columns under saturated flow conditions. pyrithiobac breakthrough curves were asymmetrical in shape with significant tailing and displaced to the left of 1 pore v ... | 1999 | 10564050 |
| interplant movement of heliothis virescens (lepidoptera: noctuidae) larvae in pure and mixed plantings of cotton with and without expression of the cry1ac delta-endotoxin protein of bacillus thuringiensis berliner. | laboratory and field studies were conducted during 1993 and 1994 to quantify interplant movement of heliothis virescens (f.) larvae in pure and mixed plantings of cotton, gossypium hirsutum l., with ('event 531') and without ('coker 312') the expression of cry1ac delta-endotoxin protein of bacillus thuringiensis berliner. field studies were conducted with neonate, 4-, and 7-d-old larvae placed on 3-plant experimental units and observed at 24, 48, 72, and 96 h after inoculation of larvae. combini ... | 1999 | 10504897 |
| hmg-coa reductase gene family in cotton (gossypium hirsutum l.): unique structural features and differential expression of hmg2 potentially associated with synthesis of specific isoprenoids in developing embryos. | as a first step towards understanding the biosynthesis of isoprenoids that accumulate in specialized pigment glands of cotton at the molecular level, two full-length genes (hmg1 and hmg2) were characterized encoding hmg-coa reductase (hmgr; ec 1.1.1.34), the enzyme that catalyzes the formation of a key isoprenoid precursor. cotton hmgr genes exhibited features typical of other plant genes, however, hmg2 encodes the largest of all plant hmgr enzymes described to date. hmg2 contains several novel ... | 1999 | 10501034 |
| gene-specific changes in alpha-tubulin transcript accumulation in developing cotton fibers. | the fibers of cotton (gossypium hirsutum) are single-cell trichomes that undergo rapid and synchronous elongation. cortical microtubules provide spatial information necessary for the alignment of cellulose microfibrils that confine and regulate cell elongation. we used gene-specific probes to investigate alpha-tubulin transcript levels in elongating cotton fibers. two discrete patterns of transcript accumulation were observed. whereas transcripts of alpha-tubulin genes ghtua2/3 and ghtua4 increa ... | 1999 | 10482673 |
| conditioning of fasciation by gibberellin and genotype in cotton (gossypium hirsutum l.). | the round-leafed mutant cotton line l-501 developed fasciation of the upper stem when field grown in central asia. fasciation co-segregated with the mutant gene for round leaves in.(l) fasciation developed at the flowering stage, but removal of floral buds did not prevent fasciation. fasciation in l-501 could be inhibited by the gibberellin (ga) biosynthesis inhibitor chlorocholine chloride or by fusicoccin. ga(3) application in the field induced fasciation in the mutant's parental line l-463, w ... | 1999 | 10467019 |
| identification of disease response genes expressed in gossypium hirsutum upon infection with the wilt pathogen verticillium dahliae. | verticillium wilt is a vascular disease of cotton (gossypium spp.) caused by the fungal pathogen verticillium dahliae. to begin to understand the molecular mechanisms of the disease response in cotton cultivars that display superior wilt tolerance, such as gossypium hirsutum cv. sicala v-1, a cdna library was constructed with mrna isolated from root tissue of sicala v-1, 24 h after inoculation with v. dahliae. the library was screened by a differential screening technique which was successful in ... | 1999 | 10412907 |
| new ribosomal rna gene locations in gossypium hirsutum mapped by meiotic fish. | in this study we have mapped newly identified rdna loci in gossypium hirsutum. four new minor 18s-26s rdna loci, in addition to the sites previously identified, were mapped using fluorescence in situ hybridization (fish) to heterozygous translocation (nt) quadrivalents (ivs). the newly detected 18s-26s rdna loci were mapped to the right arms of chromosomes 8, 9, 15, 17, 19, 20, and 23 and the left arms of chromosomes 5, 11, 12, and 14. using the rdna loci as common reference points, we detected ... | 1999 | 10398849 |
| differential regulation of six novel myb-domain genes defines two distinct expression patterns in allotetraploid cotton (gossypium hirsutum l.). | a pcr-based strategy was employed to identify myb-related genes potentially involved in the differentiation and development of cotton seed trichomes. cdna clones representing six newly identified cotton myb-domain genes (ghmyb) of the r2r3-myb family were characterized in the allotetraploid species gossypium hirsutum l. (2n = 4x = 52; aadd). several interesting motifs and domains in the transregulatory region (trr) were identified as potential candidates for modulating ghmyb activity. one such s ... | 1999 | 10394903 |
| increased nuclear dna content in developing cotton fiber cells. | the nuclear dna content of developing cotton fiber cells (gossypium hirsutum, cv. md51ne) increases ~24% after 2 d postanthesis (dpa). the amount of nuclear dna at 2 dpa is 5.4 +/- 0.27 pg. at 3-4 dpa it increases to 6.7 +/- 0.24 pg and by 5 dpa it is 6.8 +/- 0.70 pg. these values were obtained by nuclear fluorescence after staining with hoechst 33258. human oral squamous cell nuclei were used as a dna standard. nuclear dna content increases in fibers growing on either fertilized or unfertilized ... | 1999 | 10371719 |
| low levels of nucleotide diversity at homoeologous adh loci in allotetraploid cotton (gossypium l.). | levels of genetic diversity within and among populations and species are shaped by both external (population-level) and internal (genomic and genic) evolutionary forces. to address the effect of internal pressures, we estimated nucleotide diversity for a pair of homoeologous adh loci in an allotetraploid species, gossypium hirsutum. these data represent the first such estimates for a pair of homoeologous nuclear loci in plants. estimates of nucleotide diversity for adha in gossypium are lower th ... | 1999 | 10331275 |
| inhibition and acclimation of photosynthesis to heat stress is closely correlated with activation of ribulose-1,5-bisphosphate carboxylase/oxygenase | increasing the leaf temperature of intact cotton (gossypium hirsutum l.) and wheat (triticum aestivum l.) plants caused a progressive decline in the light-saturated co2-exchange rate (cer). cer was more sensitive to increased leaf temperature in wheat than in cotton, and both species demonstrated photosynthetic acclimation when leaf temperature was increased gradually. inhibition of cer was not a consequence of stomatal closure, as indicated by a positive relationship between leaf temperature an ... | 1999 | 10318695 |
| coordinated accumulation of (+)-delta-cadinene synthase mrnas and gossypol in developing seeds of gossypium hirsutum and a new member of the cad1 family from g. arboreum. | a new member of the (+)-delta-cadinene synthase (cad1) family was isolated from a gossypium arboreum cdna library. this cdna encodes a protein that showed 97.3%, 96.9%, and 79.2% sequence identities with the proteins encoded by previously isolated cdnas of cad1-c1, cad1-c14, and cad1-a, respectively. it may be grouped into the cad1-c subfamily as cad1-c2. seeds of a glanded cotton cultivar, g. hirsutum cv. sumian-6, were collected at different intervals during maturation, and the cad1 mrna level ... | 1999 | 10075752 |
| glycerol is a suberin monomer. new experimental evidence for an old hypothesis | the monomer composition of the esterified part of suberin can be determined using gas chromatography-mass spectroscopy technology and is accordingly believed to be well known. however, evidence was presented recently indicating that the suberin of green cotton (gossypium hirsutum cv green lint) fibers contains substantial amounts of esterified glycerol. this observation is confirmed in the present report by a sodium dodecyl sulfate extraction of membrane lipids and by a developmental study, demo ... | 1999 | 10069853 |
| gtpase activity and biochemical characterization of a recombinant cotton fiber annexin. | a cdna encoding annexin was isolated from a cotton (gossypium hirsutum) fiber cdna library. the cdna was expressed in escherichia coli, and the resultant recombinant protein was purified. we then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. an "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. however, it was found that the recomb ... | 1999 | 10069831 |
| the involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers | h2o2 is a widespread molecule in many biological systems. it is created enzymatically in living cells during various oxidation reactions and by leakage of electrons from the electron transport chains. depending on the concentration h2o2 can induce cell protective responses, programmed cell death, or necrosis. here we provide evidence that h2o2 may function as a developmental signal in the differentiation of secondary walls in cotton (gossypium hirsutum) fibers. three lines of evidence support th ... | 1999 | 10069824 |
| isolation of a cotton cap gene: a homologue of adenylyl cyclase-associated protein highly expressed during fiber elongation. | the cdna encoding cap (adenylyl cyclase-associated protein) was isolated from a cotton (gossypium hirsutum) fiber cdna library. the cdna (ghcap) contained an open reading frame that encoded 471 amino acid residues. rna blot analysis showed that the cotton cap gene was expressed mainly in young fibers. | 1998 | 10050322 |
| heterologous myb genes distinct from gl1 enhance trichome production when overexpressed in nicotiana tabacum. | myb-class transcription factors implicated in cell shape regulation were overexpressed in arabidopsis thaliana and nicotiana tabacum in an attempt to assess the extent to which cellular differentiation programs might be shared between these distantly related plants. glabrous 1, a myb gene required for trichome development in arabidopsis, did not alter the trichome phenotype of the tobacco plants in which it was overexpressed. mixta, which in antirrhinum majus is reported to regulate certain aspe ... | 1999 | 9895315 |
| structural analysis of a hmg-coa-reductase pseudogene: insights into evolutionary processes affecting the hmgr gene family in allotetraploid cotton (gossypium hirsutum l.). | structural analysis of hmg-coa reductase (hmgr) genes in the allotetraploid cotton species gossypium hirsutum l. revealed the first-known existence of a pseudogene, psihmg5, for this important enzyme. complete sequencing of the genomic clone hmg5 unveiled several deleterious lesions, resulting in an organization that departed significantly from the linear canonical hmgr gene structure. although analysis of the 5' flanking region indicated a promoter-like composition based on comparison with othe ... | 1998 | 9799357 |
| a fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds | fiber cell initiation in the epidermal cells of cotton (gossypium hirsutum l.) ovules represents a unique example of trichome development in higher plants. little is known about the molecular and metabolic mechanisms controlling this process. here we report a comparative analysis of a fiberless seed (fls) mutant (lacking fibers) and a normal (fls) mutant to better understand the initial cytological events in fiber development and to analyze the metabolic changes that are associated with the loss ... | 1998 | 9765525 |
| analysis of plastid dna-like sequences within the nuclear genomes of higher plants. | a wide-ranging examination of plastid (pt)dna sequence homologies within higher plant nuclear genomes (promiscuous dna) was undertaken. digestion with methylation-sensitive restriction enzymes and southern analysis was used to distinguish plastid and nuclear dna in order to assess the extent of variability of promiscuous sequences within and between plant species. some species, such as gossypium hirsutum (cotton), nicotiana tabacum (tobacco), and chenopodium quinoa, showed homogenity of these se ... | 1998 | 9615455 |
| polyploid formation created unique avenues for response to selection in gossypium (cotton). | a detailed restriction fragment length polymorphism map was used to determine the chromosomal locations and subgenomic distributions of quantitative trait loci (qtls) segregating in a cross between cultivars of allotetraploid (aadd) gossypium hirsutum ("upland" cotton) and gossypium barbadense ("sea island," "pima," or "egyptian" cotton) that differ markedly in the quality and quantity of seed epidermal fibers. most qtls influencing fiber quality and yield are located on the "d" subgenome, deriv ... | 1998 | 9539752 |
| genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated. | cotton (gossypium hirsutum l.) fibers are single-celled trichomes that synchronously undergo a phase of rapid cell expansion, then a phase including secondary cell wall deposition, and finally maturation. to determine if there is coordinated regulation of gene expression during fiber expansion, we analyzed the expression of components involved in turgor regulation and a cytoskeletal protein by measuring levels of mrna and protein accumulation and enzyme activity. fragments of the genes for the p ... | 1998 | 9536073 |
| moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase-mediated activation of rubisco | we tested the hypothesis that light activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) is inhibited by moderately elevated temperature through an effect on rubisco activase. when cotton (gossypium hirsutum l.) or wheat (triticum aestivum l.) leaf tissue was exposed to increasing temperatures in the light, activation of rubisco was inhibited above 35 and 30 degreesc, respectively, and the relative inhibition was greater for wheat than for cotton. the temperature-induced inhib ... | 1998 | 9490757 |
| manipulation of catalase levels produces altered photosynthesis in transgenic tobacco plants. | constructs containing the cdnas encoding the primary leaf catalase in nicotiana or subunit 1 of cottonseed (gossypium hirsutum) catalase were introduced in the sense and antisense orientation into the nicotiana tabacum genome. the n. tabacum leaf cdna specifically overexpressed cat-1, the high catalatic [corrected] form, activity. antisense constructs reduced leaf catalase specific activities from 0.20 to 0.75 times those of wild type (wt), and overexpression constructs increased catalase specif ... | 1998 | 9449845 |
| identification of a delta-tip cdna clone and determination of related a and d genome subfamilies in gossypium species. | tonoplast intrinsic proteins (tips) have been implicated in the process of cell elongation, such as occurs in the developing cotton fiber. we have isolated a cdna clone (997 bp in length) from a cotton (gossypium hirsutum l.) library which putatively encodes a protein of 248 residues (mr 25079) with 85% identity to arabidopsis delta-tip. the derived amino acid sequence included two conserved sequences associated with major intrinsic proteins (sgxhxnpa at residues 78 to 85, npa residues at 197 to ... | 1997 | 9177317 |
| sequence and rt-pcr expression analysis of two peroxidases from arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases. | cdna clones encoding two new arabidopsis thaliana peroxidases, atp 1a and atp 2a, have been identified by searching the arabidopsis database of expressed sequence tags (dbest). they represent a novel branch of hitherto uncharacterized plant peroxidases which is only 35% identical in amino acid sequence to the well characterized group of basic plant peroxidases represented by the horseradish (armoracia rusticana) isoperoxidases hrp c, hrp e5 and the similar arabidopsis isoperoxidases atp ca, atp ... | 1997 | 9132061 |
| identification of a cotton fiber-specific acyl carrier protein cdna by differential display. | transcripts from immature fibers and stripped ovules (fibers removed) of cotton (gossypium hirsutum l.) were compared by differential display to identify cdna fragments that represent mrnas that are expressed primarily in cotton fibers. eight independent fiber-specific cdna fragments were isolated. one of these cdnas had strong sequence similarity with acyl carrier protein (acp). a full-length cdna for the cotton fiber-specific acp was isolated using a pcr cdna library screening technique. this ... | 1997 | 9130594 |
| use of meiotic fish for identification of a new monosome in gossypium hirsutum l. | the extensive use of molecular cytogenetics in human genetics and clinical diagnostics indicates that analogous applications in plants are highly feasible. one sort of application would be the identification of new aneuploids, which traditionally involves either direct karyotypic identification, which is feasible in only a few plant species, or tests with markers (cytogenetic, genetic, or molecular), which require sexual hybridization and at least one subsequent seed or plant generation. we have ... | 1997 | 9061912 |
| higher plants contain homologs of the bacterial cela genes encoding the catalytic subunit of cellulose synthase. | in spite of much effort, no one has succeeded in isolating and characterizing the enzyme(s) responsible for synthesis of cellulose, the major cell wall polymer of plants. we have characterized two cotton (gossypium hirsutum) cdna clones and identified one rice (oryza sativa) cdna that are homologs of the bacterial cela genes that encode the catalytic subunit of cellulose synthase. three regions in the deduced amino acid sequences of the plant cela gene products are conserved with respect to the ... | 1996 | 8901635 |
| ribosomal protein rl44 is encoded by two subfamilies in upland cotton (gossypium hirsutum l.). | we have isolated 4 cdna clones encoding the full-length sequence of the eukaryotic ribosomal protein rl44 from upland cotton (gossypium hirsutum l.). sequencing of these clones resulted in the classification of 2 subfamilies of rl44; these subfamilies had coding regions (315 bp) which were 92% identical. rl44-1 (454 bp) and rl44-2 (485 bp) constitute subfamily 1, whereas rl44-3 (913 bp) and rl44-5 (541 bp) constitute subfamily 2. the differences in nucleotide sequences, however, occurred only at ... | 1996 | 8806588 |
| characterization and expression of chitinase and 1,3-beta-glucanase genes in cotton. | we have isolated cdna clones representing mrnas encoding chitinase and 1,3-beta-glucanase in cotton (gossypium hirsutum l.) leaves. the chitinase clones were sequenced and found to encode a 28,806 da protein with 71% amino acid sequence similarity to the sk2 chitinase from potato (solanum tuberosum). the 1,3-beta-glucanase clones encoded a 37,645 da protein with 57.6% identity to a 1,3-beta-glucanase from soybean (glycine max). northern blot analyses showed that chitinase mrna is induced in plan ... | 1996 | 8806421 |
| the alcohol dehydrogenase genes of cotton. | we report here the initial characterization of the alcohol dehydrogenase (adh) gene family of the allotetraploid gossypium hirsutum, a crop plant that is highly sensitive to waterlogging. twelve adh cdnas were isolated from a library constructed from rna prepared from anaerobically stressed root tips. nine of the twelve cdnas fell into one class, while each of the other three cdnas fell into a separate class. the 3'-untranslated regions had little or no homology between classes implying that eac ... | 1996 | 8806419 |
| characterization and expression of metallothionein-like genes in cotton. | we have characterized cotton (gossypium hirsutum l.) genes encoding type 1 metallothionein-like proteins that are highly expressed in roots. little or no expression of these genes was detected in other organs and tissues. the deduced amino acid sequences have a high degree of similarity with type 1 metallothionein-like proteins from other plants, including a central hydrophobic domain flanked by conserved cysteine-rich motifs. the type 1 metallothionein-like genes of cotton are encoded by a smal ... | 1996 | 8790303 |
| purification of (+)-delta-cadinene synthase, a sesquiterpene cyclase from bacteria-inoculated cotton foliar tissue. | a sesquiterpene cyclase whose activity is induced in a glandless, bacterial blight-resistant line of cotton (gossypium hirsutum l.) catalyses the conversion of (e,e)-farnesyl diphosphate to (+)-delta-cadinene. this enzyme was purified by a combination of salt-induced phase separation, hydroxylapatite fractionation, hydrophobic interaction and strong anion-exchange chromatography, and denaturing polyacrylamide gel electrophoresis, followed by renaturation with tween 80. the purified enzyme has a ... | 1996 | 8728715 |
| differential induction of 3-hydroxy-3-methylglutaryl coa reductase in two cotton species following inoculation with verticillium. | gossypium barbadense cottons are typically more resistant to wilt pathogens than are gossypium hirsutum cultivars. both species make terpenoid phytoalexins in response to infection, implicating isoprenoid biosynthesis as a factor in resistance. conserved regions in plant 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr), the first enzyme in the terpene biosynthesis pathway, were used to design polymerase chain reaction primers for cloning a fragment of a cotton hmgr gene. the clone was used ... | 1995 | 8664497 |
| distribution of 5s and 18s-28s rdna loci in a tetraploid cotton (gossypium hirsutum l.) and its putative diploid ancestors. | the most widely cultivated species of cotton, gossypium hirsutum, is a disomic tetraploid (2n=4x=52). it has been proposed previously that extant a- and d-genome species are most closely related to the diploid progenitors of the tetraploid. we used fluorescent in situ hybridization (fish) to determine the distribution of 5s and 18s-28s rdna loci in the a-genome species g. herbaceum and g. arboreum, the d-genome species g. raimondii and g. thurberi, and the ad tetraploid g. hirsutum. high signal- ... | 1996 | 8662259 |
| structural characterization of genes corresponding to cotton fiber mrna, e6: reduced e6 protein in transgenic plants by antisense gene. | two genes, each corresponding to fiber mrna e6, were isolated from cotton cultivars coker 312 (gossypium hirsutum l.) and sea island (g. barbadense l.). e6 is one of the predominant fiber-specific mrnas present during early fiber development. the distinguishing feature of nucleotide-derived e6 protein is the presence of a motif where a dimer, ser-gly, is repeated several times. two of the sea island genes contained a pentameric motif, ser-gly, while one of the coker genes had one and the other h ... | 1996 | 8616253 |
| diversity in cyclic sesquiterpene production by gossypium hirsutum. | major sesquiterpene components of oil of texas race stock 810 of gossypium hirsutum were alpha- and beta-selinene. this is the seventh cyclic terpene type found to date in this genus. both alpha- and beta-selinene, along with aromadendrene, were found but only as minor components of extracts of several domestic cultivars of g. hirsutum. | 1995 | 8590634 |
| permanent gases inside healthy and microbially infected cotton fruit during development. | permanent gases inside developing cotton fruit (gossypium hirsutum l.) were, by weight, 46% nitrogen, 29% oxygen, 4% argon and 20% carbon dioxide, whereas plant canopy air assayed at 73% nitrogen, 25% oxygen, 2% argon and 0.3% carbon dioxide. light exposure, fruit age, and mild infection (erwinia) had no compositional effect but aggressive infection (aspergillus) raised carbon dioxide content to 31% by weight and correspondingly lowered oxygen to 17%. respiration with oxygen replenishment except ... | 1993 | 8466505 |
| cotton (gossypium hirsutum) matp6 and matp7 oleosin genes. | 1993 | 8278515 | |
| toxicity of cotton phytoalexins to zoopathogenic fungi. | the sesquiterpenoid phytoalexins desoxyhemigossypol, desoxymethoxyhemigossypol, and hemigossypolone formed in cotton (gossypium hirsutum and g. barbadense) stem xylem infected with verticillium dahliae were shown to be highly toxic to zoopathogenic fungi. this appears to be the first study of the toxicity of terpenoid phytoalexins to zoopathogenic fungi. the toxicities of the phytoalexins expressed as mic (micrograms ml-1) values were 8 to 128 against four isolates of candida albicans and one is ... | 1993 | 8167949 |
| [nucleotide sequence of internal transcribed spacers and 5.8s rdna for the ribosomal operon from alfalfa medicago sativa and cotton gossypium hirsutum l]. | the 708- and 769-bp fragments from alfalfa and cotton containing the 3'-end of the 18s gene, the internal transcribed spacer. 1 (its1) the 5.8s gene, its2, and the 5'-end of the 28s gene were obtained using primers to the 18s and 28s ends of rdna from tomato by a polymerase chain reaction. these sequences were cloned into ptz19r. the 5.8s rdna, its1 and its2 nucleotide sequences of alfalfa and cotton were determined. comparative analysis of nucleotide sequences of alfalfa and cotton showed large ... | 2006 | 8145750 |
| vacuolar h(+)-atpase 69-kilodalton catalytic subunit cdna from developing cotton (gossypium hirsutum) ovules. | 1993 | 8108515 | |
| a method for the isolation of nuclear dna from cotton (gossypium) leaves. | cotton leaves contain high levels of polyphenolic compounds that irreversibly interact with proteins and nucleic acids during dna isolation. a procedure to isolate nuclear dna from cotton (gossypium hirsutum l.) has been developed. the method is based on the rapid initial isolation of nuclei in a glucose medium designed to stabilize nuclear structure and composition while preventing covalent interactions with polyphenolics. the resulting dna is high in yield and purity and is suitable for southe ... | 1993 | 8098189 |
| phenylalanine ammonia-lyase from cotton (gossypium hirsutum) hypocotyls: properties of the enzyme induced by a verticillium dahliae phytotoxin. | phenylalanine ammonia-lyase (ec 4.3.1.5), induced by a verticillium dahliae phytotoxin, has been purified to electrophoretic homogeneity from cotton hypocotyls by differential ammonium sulfate fractionation and hydrophobic interaction chromatography, with a yield of 52%. the enzyme is a tetramer with a molecular weight of 332,000 to 337,000. the isoelectric point is 4.6, and no isoforms were observed. the subunits of the enzyme are unstable and breaks down to fragments with m(r)'s of 69,000 and ... | 1994 | 8043606 |
| isolation of multiple cdnas encoding the vacuolar h(+)-atpase subunit b from developing cotton (gossypium hirsutum l.) ovules. | 1994 | 7972522 | |
| isolation and characterization of a cdna encoding ribosomal protein l41 from cotton (gossypium hirsutum l.). | 1994 | 7972506 | |
| cotton (gossypium hirsutum l.) pollen-specific polygalacturonase mrna: tissue and temporal specificity of its promoter in transgenic tobacco. | a gene (g9) expressed during late microsporogenesis in cotton (gossypium hirsutum l.) was isolated. sequence analysis of the cdna (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kda. the coding region of the gene is interrupted by three introns. northern analysis of the rna from developing anthers showed that the transcripts appear 12 days before anthesis and that the maximal concentration of rna occurs in pollen on the day of anthesi ... | 1994 | 7858233 |
| a detailed rflp map of cotton, gossypium hirsutum x gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. | we employ a detailed restriction fragment length polymorphism (rflp) map to investigate chromosome organization and evolution in cotton, a disomic polyploid. about 46.2% of nuclear dna probes detect rflps distinguishing gossypium hirsutum and gossypium barbadense; and 705 rflp loci are assembled into 41 linkage groups and 4675 cm. the subgenomic origin (a vs. d) of most, and chromosomal identity of 14 (of 26), linkage groups is shown. the a and d subgenomes show similar recombinational length, s ... | 1994 | 7851778 |
| the expression and anaerobic induction of alcohol dehydrogenase in cotton. | the alcohol dehydrogenase (adh) system in cotton is characterized, with an emphasis on the cultivated allotetraploid species gossypium hirsutum cv. siokra. a high level of adh activity is present in seed of siokra but quickly declines during germination. when exposed to anaerobic stress the level of adh activity can be induced several fold in both roots and shoots of seedlings. unlike maize and arabidopsis, adh activity can be anaerobically induced in mature green leaves. three major adh isozyme ... | 1994 | 7826315 |
| isolation and characterization of a cdna encoding ribosomal protein s16 from cotton (gossypium hirsutum l.). | 1994 | 7824648 | |
| a cdna encoding ribosomal protein s4e from cotton (gossypium hirsutum l.). | 1995 | 7784518 | |
| characterization of a cotton (gossypium hirsutum l.) fiber mrna (fb-b6). | 1995 | 7770543 | |
| simultaneous induction of postabscission and germination mrnas in cultured dicotyledonous embryos. | cloned mrnas identify three programs of gene expression in cotton (gossypium hirsutum l.) embryos that are associated with the maturation (reserve accumulation) stage, the postabscission stage, which is marked by expression of late-embryogenesis-abundant (lea) mrnas, and germination (broadly defined as including all events through early postgerminative growth). in order to test if the regulation of these programs is the same in other dicotyledonous species, their expression was studied in normal ... | 1994 | 7764404 |
| a view of plant dehydrins using antibodies specific to the carboxy terminal peptide. | dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ... | 1993 | 7693020 |
| copia-like retrotransposable element evolution in diploid and polyploid cotton (gossypium l.). | copia-like retrotransposable elements were identified in allotetraploid cotton, gossypium hirsutum, and two species representing its diploid progenitors, g. herbaceum and g. raimondii. these elements are present in high copy number in all three species. because the two diploid genomic groups have been isolated on opposite sides of the world for 6-11 million years, horizontal transfer of elements between these species is highly unlikely. elements were intensively sampled to generate a model of co ... | 1993 | 7685393 |
| solubilization and partial characterization of extensin fragments from cell walls of cotton suspension cultures. evidence for a covalent cross-link between extensin and pectin. | extensin, a major hydroxyproline (hyp)-rich glycoprotein in walls of cultured cells of dicotyledonous plants, is very difficult to solubilize. to learn about the nature of the insolubilization, we have tested the ability of a variety of selective hydrolytic methods, and combinations of them, to liberate extensin or fragments of extensin from suspension-culture cell walls. after the complete deglycosylation of cotton (gossypium hirsutum l.) walls, trypsinization solubilized 80% of the hyp. the se ... | 1995 | 7659756 |
| expression of two related vacuolar h(+)-atpase 16-kilodalton proteolipid genes is differentially regulated in a tissue-specific manner. | the 16-kd proteolipid subunit is the principal integral membrane protein of the vacuolar h(+)-atpase (v-atpase) complex that forms the proton channel responsible for translocating protons across lipid bilayers. two degenerate synthetic oligonucleotides, cot11 and cot12, corresponding to highly conserved transmembrane domains in all 16-kd subunits sequenced so far, were used to amplify a partial cdna of the v-atpase proteolipid subunit from cotton (gossypium hirsutum l.) by polymerase chain react ... | 1995 | 7659746 |
| organization, inheritance and expression of acetohydroxyacid synthase genes in the cotton allotetraploid gossypium hirsutum. | the acetohydroxyacid synthase (ahas) gene family of the cotton ad allotetraploid gossypium hirsutum has been cloned and characterized. we have identified six different ahas genes from an analysis of genomic clones and southern blots of genomic dna. four of the six genes are organized as tandem pairs, in which the genes are separated by only 2-3 kb. conservation of restriction fragment length polymorphisms between g. hirsutum and a-genome and d-genome-containing diploid cottons was sufficient to ... | 1995 | 7640356 |
| characterization of mrna for a proline-rich protein of cotton fiber. | cotton (gossypium hirsutum l.) mrna (h6) is expressed predominantly in fiber cells and is present during early primary cell wall formation. however, h6 protein is found to accumulate during later stages, when active secondary cell wall formation occurs, indicating possible regulation at the translational level and function in the secondary cell wall assembly. the nucleotide-derived amino acid sequence of pck-h6 is proline rich (35 mol %) with a calculated molecular mass of 21 kd. cotton protein ... | 1995 | 7610164 |
| a membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. | sucrose synthase (susy; ec 2.4.1.13; sucrose + udp reversible udpglucose + fructose) has always been studied as a cytoplasmic enzyme in plant cells where it serves to degrade sucrose and provide carbon for respiration and synthesis of cell wall polysaccharides and starch. we report here that at least half of the total susy of developing cotton fibers (gossypium hirsutum) is tightly associated with the plasma membrane. therefore, this form of susy might serve to channel carbon directly from sucro ... | 1995 | 7568131 |
| a modified hot borate method significantly enhances the yield of high-quality rna from cotton (gossypium hirsutum l.). | the isolation of biologically active rna from cotton (gossypium hirsutum l.) is difficult due to interference by high levels of endogenous phenolics, polysaccharides, and secondary metabolites. a modified hot borate procedure was developed to combat these cellular constituents during tissue homogenization, resulting in the quantitative recovery of rna suitable for hybridization analysis, in vitro translation, and cdna synthesis. the efficacy of several hot borate buffer adjuvants for the qualita ... | 1994 | 7535022 |
| n-acylphosphatidylethanolamine in dry and imbibing cottonseeds. amounts, molecular species, and enzymatic synthesis. | n-acylphosphatidylethanolamine (nape), an unusual acylated derivative of phosphatidylethanolamine (pe), was recently shown to be synthesized from pe and free fatty acids in cotyledons of cotton (gossypium hirsutum l.) seedlings (k.d. chapman, t.s. moore [1993] plant physiol 102: 761-769). here we report that nape is present in dry seeds of cotton and increases with time of imbibition from 2.31 nmol/seed in dry seeds to 4.26 nmol/seed in 4-h-soaked seeds. total phospholipid/seed also increased su ... | 1995 | 7480326 |
| prenyltransferase from gossypium hirsutum. | 1980 | 7436426 | |
| ultrastructural studies of protophloem sieve elements in gossypium hirsutum. | 1981 | 7277570 | |
| nuclear degeneration and the association of endoplasmic reticulum with the nuclear envelope and microtubules in maturing sieve elements of gossypium hirsutum. | 1981 | 7241642 | |
| changes in the endoplasmic reticulum during differentiation of a sieve element in gossypium hirsutum. | 1981 | 7241641 |