Publications

a rapid and reliable method for no quantification and 15no/14no determination using isotope ratio mass spectrometry: an application for the detection of no synthesis in propionibacteria.	for the last decade, numerous studies have focused on the positive or toxic effects of nitric oxide (no) in procaryotic and eucaryotic cells. this gas has fundamental roles in neurotransmission, vasodilatation, cytotoxicity, and intestinal motility. the ability to produce no by intestinal microflora or probiotic bacteria is unknown. in this preliminary study, we present a rapid and reproducible procedure for no quantification and 15no/14no determination (based on the reaction between nitrite and ...	1999	10390862
crystal structure of substrate complexes of methylmalonyl-coa mutase.	x-ray crystal structures of methylmalonyl-coa mutase in complexes with substrate methylmalonyl-coa and inhibitors 2-carboxypropyl-coa and 3-carboxypropyl-coa (substrate and product analogues) show that the enzyme-substrate interactions change little during the course of the rearrangement reaction, in contrast to the large conformational change on substrate binding. the substrate complex shows a 5'-deoxyadenine molecule in the active site, bound weakly and not attached to the cobalt atom of coenz ...	1999	10387043
antibiotic sensitivity of propionibacterium acnes isolates studied in a skin clinic in singapore.		1999	10376712
localized pericarditis with calcifications mimicking a pericardial tumor.	a 62-year-old man was admitted with increasing palpitations. radiography of the chest demonstrated a calcified mass. magnetic resonance imaging revealed compression of the right ventricle by a tumor. at the time of cardiac catheterization, the coronary arteries were found not to supply blood flow of the mass, and no dip-and-plateau pattern was seen in the right ventricular pressure measurements. at the time of surgery, the mass was found to be a focal calcified thickening of the pericardium cont ...	1999	10361909
lacticin 3147 displays activity in buffer against gram-positive bacterial pathogens which appear insensitive in standard plate assays.	lacticin 3147 is a broad-spectrum bacteriocin produced by lactococcus lactis subsp. lactis dpc3147, which has been shown to be active against a range of food-borne bacteria. the reported inhibitory range for lacticin is extended to include methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococcus faecalis, penicillin-resistant pneumococcus, propionibacterium acne and streptococcus mutans. this extended host range is not obvious from traditional agar plate-based methods, but ...	1999	10347889
gastrointestinal case of the day. pyogenic liver abscess caused by perforation by a swallowed wooden toothpick.		1999	10336207
bacteriostatic effects of hyaluronic acid.	this investigation is one of a series of projects seeking to ascertain whether hyaluronic acid (ha) is therapeutically effective in tissue regeneration procedures. the rationale for these investigations is to test the hypothesis that ha can serve as a bioabsorbable carrier for other substrates as well as itself actively promote the regeneration of tissue.	1999	10328647
[intraocular contamination during cataract surgery according to surgical technique and type of implant].	to determine wether the cataract extraction method and intraocular lens material affect bacterial contamination of the eye during surgery.	1999	10327353
in vitro activity of ly 333328 against anaerobic gram-positive bacteria.	ly 333328 is a new semisynthetic glycopeptide with reported activity against aerobic gram-positive cocci such as enterococci, pneumococci, streptococci and staphylococci. the present investigation was undertaken to determine the in vitro activity of ly 333328 against 178 gram-positive anaerobic bacteria recently isolated from human infections. the activity was compared with that of vancomycin, teicoplanin, cefoxitin, imipenem, clindamycin and metronidazole. peptostreptococci (48 strains): ly 333 ...	1999	10326737
tessaracoccus bendigoensis gen. nov., sp. nov., a gram-positive coccus occurring in regular packages or tetrads, isolated from activated sludge biomass.	an isolate of a gram-positive bacterium, designated strain ben 106t, was obtained in pure culture by micromanipulation of a biomass sample obtained from a laboratory-scale sequencing batch reactor. this isolate grew axenically as cocci or clusters of cocci arranged in regular tetrads and was morphologically similar to the dominant organism observed in the biomass. this morphology resembled that of some gram-positive and -negative bacteria and the so-called 'g-bacteria' commonly seen in activated ...	1999	10319465
propionibacterium as a cause of postneurosurgical infection in patients with dural allografts: report of three cases.	although propionibacterium acnes is a common inhabitant of human skin, it is an uncommon pathogen in postoperative infections. we report three cases of postoperative wound infection/osteomyelitis caused by p. acnes.	1999	10232551
manipulating the coordination mumber of the ferric iron within the cambialistic superoxide dismutase of propionibacterium shermanii by changing the ph-value a crystallographic analysis.	the structure of the propionibacterium freudenreichii subspecies shermanii superoxide dismutase (sod) was determined at various ph values. as a comparison, the structure of the fluoride coordinated sod was solved. the sod crystallizes at ph 6.1 in the space group c2221 with two subunits, a and b, in the asymmetric unit. an increase of the ph value changes the cell parameters slightly but not the symmetry of the crystals. the overall structure of the sod remains a compact tetrameter and is compar ...	1999	10231372
corynebacterium parvum- and mycobacterium bovis bacillus calmette and guerin-induced granuloma formation in mice lacking cd4 and cd8.	granuloma formation is a t-cell-dependent inflammatory response that is important in the host defense against intracellular bacteria. the role of cd4 and cd8 molecules in the development of corynebacterium parvum- and mycobacterium bovis bacillus calmette and guerin (bcg)-induced granulomas was examined in cd4/cd8 knockout (ko) mice. cd4/cd8 ko mice developed a greater granulomatous response to heat-killed c. parvum and heat-killed bcg than did control mice. thus, granuloma formation is not depe ...	1999	10222057
il-18-deficient mice are resistant to endotoxin-induced liver injury but highly susceptible to endotoxin shock.	il-18 is an il-1-related cytokine which shares biological functions with il-12. these include the activation of nk cells, induction of ifn-gamma production and th1 cell differentiation. in this study we analyzed the effect of il-18 deficiency on lipopolysaccharide (lps)-induced liver injury and endotoxin shock in propionibacterium acnes-primed mice. p. acnes-primed il-18-deficient (il-18ko) mice showed resistance to lps-induced liver injury. unexpectedly, p. acnes-primed il-18ko mice were highly ...	1999	10221659
[bacterial resistance during anti-acne antibiotic therapy. how to limit the risk].	the treatment of moderate to severe acne often relies on antibiotherapy in order to eradicate as much as possible microorganisms such as propionibacterium spp colonizing the sebaceous follicles. in recent years, bacterial resistances against specific antibiotics have emerged. both the antibiotic and its administration modalities must be considered in order to control the risk. with regard to this conundrum, minocycline is a medication of choice among the diverse anti-acneic therapies.	1999	10221062
coagulase-negative staphylococci are the most common bacteria found in cultures from the deep portions of hidradenitis suppurativa lesions, as obtained by carbon dioxide laser surgery.	the significance of bacterial findings in hidradenitis suppurativa (hs) is controversial. interpretation of the results of bacteriological examinations from the surface of hs lesions is obscured by the possible contamination of resident skin bacteria. bacteriological analysis of aspirates from deeper parts of hs is liable to show low sensitivity. we used a carbon dioxide (co2) laser method to evaporate the diseased tissue level by level from the surface downwards, allowing concurrent sampling of ...	1999	10215774
propionibacterium acnes: interaction with complement and development of an enzyme-linked immunoassay for the detection of antibody.	to characterize the immune response to propionibacterium acnes in acne patients.	1999	10208616
functional roles of mcp-1 in propionibacterium acnes-induced, t cell-mediated pulmonary granulomatosis in rabbits.	the immunological manifestation of granuloma formations in humans largely depends on the delayed-type hypersensitivity response. we investigated the involvement of monocyte chemoattractant protein-1 (mcp-1) in a rabbit model of t cell-mediated pulmonary granulomatosis. intravenous injection of propionibacterium acnes (p. acnes) into sensitized rabbits induced massive and diffuse pulmonary granulomas. levels of mcp-1 in sera and bronchoalveolar lavage fluids (balf) peaked before the granuloma for ...	1999	10204577
human ileostomy glycoproteins as a model for small intestinal mucus to investigate adhesion of probiotics.	human ileostomy glycoproteins were used as a model for small intestinal mucus to investigate the adhesion of 12 lactobacillus strains, one lactococcus strain and one propionibacterium strain, both probiotic and dairy strains were tested. adhesive and non-adhesive escherichia coli strains were used as controls. all the strains were also tested for their adhesion to polystyrene. adhesion to ileostomy glycoproteins and to polystyrene varied significantly among the strains tested. lactobacillus rham ...	1999	10196761
metabolic pathway of propionibacterium growing with oxygen: enzymes, 13c nmr analysis, and its application for vitamin b12 production with periodic fermentation.	the metabolic pathway of propionibacterium grown under an aerobic condition is still not clear so far. in this work, cell growth, organic acid formation, vitamin b12 synthesis, and enzyme activities were determined in different aerobic cultivation systems. it was found that the propionate, which is accumulated during anaerobic cultivation, was completely decomposed when the cultivation was shifted to an aerobic condition. moreover, pyruvate was formed in accordance with the decomposition of the ...	1999	10194395
effect of propionibacteria supplementation on fecal bifidobacteria and segmental colonic transit time in healthy human subjects.	some strains of propionibacterium have bifidogenic properties and enhance gut motility in the animal. however, they are not part of the indigenous fecal flora. this study was designed to assess the digestive survival of ingested propionibacteria, their bifidogenic properties, and the resulting changes in colonic transit time in healthy humans.	1999	10192191
acne neonatorum: a study of 22 cases.	acne is not uncommon in the neonatal period. acne neonatorum is characterized by a mainly facial eruption of inflammatory and noninflammatory lesions. it is most commonly mild and transient. hyperactivity of sebaceous glands, stimulated by neonatal androgens, has been implicated as the underlying pathogenetic mechanism.	1999	10192162
defective interleukin (il)-18-mediated natural killer and t helper cell type 1 responses in il-1 receptor-associated kinase (irak)-deficient mice.	interleukin (il)-18 is functionally similar to il-12 in mediating t helper cell type 1 (th1) response and natural killer (nk) cell activity but is related to il-1 in protein structure and signaling, including recruitment of il-1 receptor-associated kinase (irak) to the receptor and activation of c-jun nh2-terminal kinase (jnk) and nuclear factor (nf)-kappab. the role of irak in il-18-induced responses was studied in irak-deficient mice. significant defects in jnk induction and partial impairment ...	1999	10190904
possible implication of sterile connecting device in contamination of pooled platelet concentrates.	considering the possibility that a pooled random donor platelet concentrate could become contaminated by welding with a sterile connecting device, we undertook a study to determine the influence of pooling on the contamination rate. as a control group, apheresis platelets were examined. bacteriological testing was done with a sensitive co2 detecting culture system, the bact/ alert. out of 1105 pooled platelet concentrates prepared by the buffy coat method, 15 (1.4%) were confirmed as contaminate ...	1997	10175151
in vitro antibacterial properties of pexiganan, an analog of magainin.	pexiganan, a 22-amino-acid antimicrobial peptide, is an analog of the magainin peptides isolated from the skin of the african clawed frog. pexiganan exhibited in vitro broad-spectrum antibacterial activity when it was tested against 3,109 clinical isolates of gram-positive and gram-negative, anaerobic and aerobic bacteria. the pexiganan mic at which 90% of isolates are inhibited (mic90) was 32 micrograms/ml or less for staphylococcus spp., streptococcus spp., enterococcus faecium, corynebacteriu ...	1999	10103181
endogenous mouse interleukin-10 is up-regulated by exogenously administered recombinant human interleukin-10, but does not contribute to the efficacy of the human protein in mouse models of endotoxemia.	in murine models of experimental endotoxemia, inflammatory cytokines as well as antiinflammatory interleukin-10 (il-10) appear in the circulation after the injection of lipopolysaccharide (lps). there is considerable experimental evidence to suggest that the major function of endogenously produced il-10 is to down-regulate inflammatory cytokine production. indeed, the protective effects of exogenously administered il-10 against murine endotoxin lethality have been shown to correlate with its abi ...	1999	10102793
propionic acid production by extractive fermentation. i. solvent considerations.	solvent selection for extractive fermentation for propionic acid was conducted with three systems: alamine 304-1 (trilaurylamine) in 2-octanol, 1-dodecanol, and witcohol 85 nf (oleyl alcohol). among them, the solvent containing 2-octanol exhibited the highest partition coefficient in acid extraction, but it was also toxic to propionibacteria. the most solvent-resistant strain among five strains of the microorganism was selected. solvent toxicity was eliminated via two strategies: entrapment of d ...	1998	10099222
in vitro activity of 6 antimicrobials against propionibacteria isolates from untreated acne papulopustulosa.	in the present study, mic values of 6 antibiotics were determined for 70 propionibacterium acnes and 13 p.granulosum strains from 71 untreated acne patients using the agar dilution method. the interpretation of in vitro results is difficult because there are only poor data about the concentrations of antibiotics achievable in the sebaceous gland infundibulum. based on breakpoint concentrations according to din 58,944, no resistance was found against chlortetracycline, doxycycline, minocycline, a ...	1999	10096167
brain energy stores in c57bl/6 mice after c. parvum injection.	activation of the immune system has been associated with the development of fatigue of unknown cause. we were interested in brain energy stores (e.g., phosphocreatine (pcr) and creatine kinase) after immune activation to investigate whether this system was altered. in this model, fatigue (defined as > 50% reduction in spontaneous running) was induced in c57bl/6 mice after a single injection of corynebacterium parvum antigen. maximal fatigue (about 86% reduction on day 10 post injection) was asso ...	1999	10094158
prevalence of nim genes in anaerobic/facultative anaerobic bacteria isolated in south africa.	this study investigated the prevalence of nim genes (proposed to encode a 5-nitroimidazole resistance product) in 64 anaerobic/facultative anaerobic bacteria. employing universal nim gene primers, 458-bp amplified fragments were recorded as presumptive positives in 22/64 strains at an annealing temperature of 52 degrees c and 15/64 strains at 62 degrees c, of which seven were propionibacteria. dna sequencing confirmed the presence of nima genes in propionibacterium spp. (five strains), actinomyc ...	1999	10079531
role of macrophage scavenger receptors in hepatic granuloma formation in mice.	in mice homozygous for the gene mutation for type i and type ii macrophage scavenger receptors (msr-a), msr-a-/-, the formation of hepatic granulomas caused by a single intravenous injection of heat-killed corynebacterium parvum was delayed significantly for 10 days after injection, compared with granuloma formation in wild-type (msr-a+/+) mice. in the early stage of granuloma formation, numbers of macrophages and their precursor cells were significantly reduced in msr-a-/- mice compared with ms ...	1999	10079248
peritoneal dialysis-associated peritonitis caused by propionibacteria species.	there are an increasing number of reports about unusual causes of peritonitis in peritoneal dialysis (pd) patients. the propionibacteria species is a microorganism that is a normal skin flora. under the presence of certain risk factors, it may produce serious infections. patients at risk of having propionibacteria sp infections have malignancy, diabetes mellitus, foreign bodies, or immunodeficiency. we describe a pd-associated peritonitis in a 51-year-old woman that was caused by propionibacteri ...	1999	10074606
late complications of silastic duraplasty: low-virulence infections. case report.	the authors describe three patients with expanding hemorrhagic mass lesions who presented 13 to 18 years after undergoing silastic duraplasty. in all patients, results of bacteriological cultures of the masses obtained intraoperatively were positive, revealing low-virulence bacteria. two of the patients were treated with antibiotic drugs and made a good recovery. the third did not receive antibiotic medications initially and later developed an epidural empyema that necessitated reoperation, but ...	1999	10067930
[therapy-refractory iridocyclitis in pseudophakia. chronic low-grade endophthalmitis after cataract operation, induced by propionibacteria].		1999	10067335
the effect of sucrose or starch-based diet on short-chain fatty acids and faecal microflora in rats.	an investigation was carried out to determine whether variations of dietary carbohydrates could modify the colonic flora in rats. sprague-dawley rats were fed with two equicaloric diets based on the ain-76 diet (american institute of nutrition 1977) but differing from that diet in content of carbohydrates, i.e. high sucrose (64%) of high corn starch (64%). feeding was continued for 9 months ad libitum and no variation in weight gain was recorded among the different diets. a prevalence of aerobes ...	1999	10063624
resistance to antibiotics used in dermatological practice.	the increased prevalence of bacterial resistance is one of the major problems of medicine today. antibiotic resistance can be defined as the situation where the minimal inhibitory concentration is greater than the concentration obtainable in vivo. resistance genes are easily transferred among bacteria, especially bacteria on skin and mucous membranes. in dermatological patients the most important resistance problems are found among staphylococci, propionibacterium acnes and, to some extent, stre ...	1998	9990406
[the resistance of propionibacterium acnes and staphylococcus epidermidis to cyclines. the research and study group on acne].	a rising percentage of tetracycline-resistant propionibacterium acnes strains has been reported in the english literature.	1998	9922860
[in vitro resistance of propionibacterium acnes and staphylococcus epidermidis to antibiotics: should our antibiotic prescription practices for the treatment of acne be changed?].		1998	9922858
antimicrobial susceptibility of propionibacterium acnes isolated from acne vulgaris.	systemic and topical antimicrobial treatment for acne vulgaris remains the mainstay method of therapy in japan. strains of propionibacterium acnes (p. acnes) resistant to erythromycin (em), clindamycin (cldm), tetracycline (tc), doxycycline (doxy) and minocycline (mino) have been reported. the aim of the present study was to examine the antimicrobial susceptibility to 10 currently used antimicrobial agents of 50 strains of p. acnes isolated from acne lesions and identified using a rap id ana ii ...	1999	9920982
alleviation of lipopolysaccharide-induced acute liver injury in propionibacterium acnes-primed ifn-gamma-deficient mice by a concomitant reduction of tnf-alpha, il-12, and il-18 production.	the present study was designed to investigate the role of ifn-gamma in lps-induced liver injury following priming with propionibacterium acnes. at 1 week after priming balb/c mice with p. acnes, a large number of macrophages (mphi) and lymphocytes predominantly infiltrated the portal area, resulting in the intrahepatic formation of granulomas consisting of epithelioid and lymphoid cells. in comparison, in ifn-gamma gene-disrupted balb/c mice (ifn-gamma knockout mice), the number of infiltrated m ...	1999	9916732
polymerase chain reaction in the diagnosis of bacterial endophthalmitis.	microbiological investigations of vitreous fluid (vf) and aqueous humour (ah) specimens have often failed to detect the infecting agent in infectious endophthalmitis, resulting in a clinical dilemma regarding therapy. in this study, the polymerase chain reaction (pcr) was evaluated in the diagnosis of bacterial and propionibacterium acnes endophthalmitis.	1998	9893601
prospective evaluation of external ocular microbial growth and aqueous humor contamination during cataract surgery.	to analyze the route of aqueous humor contamination leading to the development of postoperative endophthalmitis.	1999	9888079
oropharynx microbiota among alcoholics and non-alcoholics.	the oropharynx microbiota plays an important role in the origin of infections, especially among alcoholics whose airway defenses are impaired.	1998	9876451
identification and clustering of dairy propionibacteria by rapd-pcr and cge-rea methods.	a total of 67 classical propionic acid bacteria (pab) strains, 10 of which were from type culture collections and 57 from milk, typical italian cheeses, acid whey and feed flour of different regions, were analysed by randomly amplified polymorphic dna (rapd-pcr) and by conventional gel electrophoresis restriction endonuclease analysis (cge-rea). the genotypic traits achieved using rapd-pcr with three primers (opl-01, opl-02 and opl-05) and smai cge-rea patterns were compared by numerical analysi ...	1998	9871315
pacemaker-induced endocarditis due to propionibacterium acnes.		1998	9868683
a clinical, microbiological, and histopathologic study of trichostasis spinulosa.	trichostasis spinulosa (tss) is a relatively common follicular disorder that can occur on the face and trunk, especially in the interscapular area. its cause remains unclear. we examined clinically 30 patients with tss and follicular materials extracted from each patient were examined microscopically. bacterial culture and skin biopsy were done in 12 and 10 patients, respectively. periodic acid schiff (pas) and brown-brenn gram stain were used for detection of pityrosporum (malassezia) and bacte ...	1998	9863280
[effect of matrine on mouse hepatitis and tumor necrosis factor production induced by propionibacterium acnes/lipopolysaccharides].	the effect of matrine (mat) on lipopolysaccharides (lps)-induced fatal hepatitis and tumor necrosis factor (tnf) production in propionibacterium acnes (pa)-primed mice were studied. mice were injected i.p. lps (10 micrograms/mouse) 7 d after i.p. pa (0.5 ml/mouse) to induce fatal hepatitis. after i.p. lps, serum tnf activity rose to 1657 +/- 406 ku.l-1 at 1.5 h and alt activity increased up to 1,496 +/- 890 u.l-1 at 5 h. six of 8 mice died within 5 h and the massive hemorrhagic necrosis of the l ...	1996	9863231
[skin reactions to antigens of propionibacterium acnes in patients with acne vulgaris treated with autovaccine].	one of the most common diseases of the skin is acne. the etiology and pathogenesis of acne, in spite of the advancement of medical knowledge, remain unknown and the effects of treatment unsatisfactory. the mechanism of the beneficial effects of immunotherapy in some cases of acne, including autovaccines prepared from the bacterial strains of the patient, also awaits explanation. the present work was aimed at elucidating the influence of autovaccine on some parameters of specific humoral and cell ...	1998	9857532
[propionibacterium acnes infectious endocarditis].	propionibacterium acnes, a gram positive microaerophilic bacteria is considered to have a low level of virulence. nevertheless, it can be associated with infective endocarditis. we report 2 cases and a review of the literature.	1998	9856128
the pyrophosphate-dependent phosphofructokinase of the protist, trichomonas vaginalis, and the evolutionary relationships of protist phosphofructokinases.	the pyrophosphate-dependent phosphofructokinase (ppi-pfk) of the amitochondriate protist trichomonas vaginalis has been purified. the enzyme is a homotetramer of about 50 kda subunits and is not subject to allosteric regulation. the protein was fragmented and a number of peptides were sequenced. based on this information a pcr product was obtained from t. vaginalis gdna and used to isolate corresponding cdna and gdna clones. southern analysis indicated the presence of five genes. one open readin ...	1998	9847416
identification of classical propionibacterium species using 16s rdna-restriction fragment length polymorphisms.	the phenotypic identification of the classical propionibacteria is essentially still problematic and alternative techniques for the identification of the various species are required. a rapid and sensitive technique for the routine identification of the classical propionibacteria, based on the amplification of 16s rrna genes using the polymerase chain reaction and the subsequent restriction endonuclease digestion of the pcr products, was previously described. although this technique enabled diff ...	1998	9841128
pivotal role of tarc, a cc chemokine, in bacteria-induced fulminant hepatic failure in mice.	thymus and activation-regulated chemokine (tarc) is a recently identified lymphocyte-directed cc chemokine which specifically chemoattracts t helper type 2 cd4(+) t cells in human. to establish the pathophysiological roles of tarc in vivo, we investigated whether a monoclonal antibody (mab) against tarc could inhibit the induction of hepatic lesions in murine model using propionibacterium acnes and lipopolysaccharide (lps). p. acnes-induced intrahepatic granuloma formation in the priming phase i ...	1998	9835618
cloning, expression, and chromosomal stabilization of the propionibacterium shermanii proline iminopeptidase gene (pip) for food-grade application in lactococcus lactis.	proline iminopeptidase produced by propionibacterium shermanii plays an essential role in the flavor development of swiss-type cheeses. the enzyme (pip) was purified and characterized, and the gene (pip) was cloned and expressed in escherichia coli and lactococcus lactis, the latter species being an extensively studied, primary cheese starter culture that is less fastidious in its growth condition requirements than p. shermanii. the levels of expression of the pip gene could be enhanced with a f ...	1998	9835556
the anti-lipase activity of shiunko on propionibacterium acnes.		1998	9832288
propionibacterium acnes sepsis in a previously healthy man.		1998	9827299
microorganisms in closed periapical lesions.	the purpose of this study was to investigate the microorganisms of strictly selected closed periapical lesions associated with both refractory endodontic therapy and pulpal calcification. definitive criteria were established that assured complete clinical isolation of the periapical lesion from the oral and periodontal environment. a total of 13 criteria-referenced lesions were selected from 70 patients with endodontic surgical indications. a well controlled culturing method was used in all case ...	1998	9823127
bacterial eradication from root dentine by ultrasonic irrigation with sodium hypochlorite.	the study aimed to evaluate intracanal irrigation procedures in eradicating bacteria from surface, shallow and deep layers of root dentine using extracted human teeth. artificial bacterial smear layer was successfully produced by rubbing a mixture of dental plaque and artificially decalcified dentine or carious dentine on root canal walls. the reservoir holes were 3.5 mm in depth, 1 mm in diameter prepared 1.5 mm apart and parallel to the root canals on the decrowned planes, in which five separa ...	1998	9823113
[bacteremia in intra-arterial angiography, percutaneous transluminal angioplasty and percutaneous transhepatic cholangio-drainage].	prospective evaluation of the rate of bacteremia attributed to invasive radiological techniques.	1998	9819654
humic acid reduction by propionibacterium freudenreichii and other fermenting bacteria	iron-reducing bacteria have been reported to reduce humic acids and low-molecular-weight quinones with electrons from acetate or hydrogen oxidation. due to the rapid chemical reaction of amorphous ferric iron with the reduced reaction products, humic acids and low-molecular-weight redox mediators may play an important role in biological iron reduction. since many anaerobic bacteria that are not able to reduce amorphous ferric iron directly are known to transfer electrons to other external accept ...	1998	9797315
suppression of murine endotoxin response by e5531, a novel synthetic lipid a antagonist.	as a consequence of blood-borne bacterial sepsis, endotoxin or lipopolysaccharide (lps) from the cell walls of gram-negative bacteria can trigger an acute inflammatory response, leading to a series of pathological events and often resulting in death. to block this inflammatory response to endotoxin, a novel lipid a analogue, e5531, was designed and synthesized as an lps antagonist, and its biological properties were examined in vitro and in vivo. in murine peritoneal macrophages, e5531 inhibited ...	1998	9797210
structural characterization of the entire 1.3s subunit of transcarboxylase from propionibacterium shermanii.	transcarboxylase (tc) from propionibacterium shermanii, a biotin-dependent enzyme, catalyzes the transfer of a carboxyl group from methylmalonyl-coa to pyruvate in two partial reactions. within the multisubunit enzyme complex, the 1.3s subunit functions as the carboxyl group carrier. the 1.3s is a 123-amino acid polypeptide (12.6 kda), to which biotin is covalently attached at lys 89. we have expressed 1.3s in escherichia coli with uniform 15n labeling. the backbone structure and dynamics of the ...	1998	9792103
effect of fk506 on the activation state of hepatic macrophages in propionibacterium acnes-treated rats.	activated hepatic macrophages can provoke massive liver necrosis following endotoxin stimulation through microcirculatory disturbances due to sinusoidal fibrin deposition in rats pretreated with heat-killed propionibacterium acnes. in these rats, fk506 (tachlorinus) administered 24 h before and at the time of endotoxin injection, significantly attenuated liver injury compared with the rats given no fk506. the effect of fk506 on hepatic macrophage activation and its action sites were studied in p ...	1998	9792034
[isolation and purification of a protective protein from propionibacterium freudenreichii subsp. shermanii].	a protein responsible for the protective and reactivating activities of two active fractions (af1 and af2) of the cells of propionibacterium freudenreichii subsp. shermanii was isolated. the active fraction af1 was obtained by fractional precipitation of the cell-free extract of propionic acid bacteria between 20 and 40% ammonium sulfate saturation, whereas fraction af2 was precipitated between 60 and 80% saturation. further fractionation of af1 and af2 by gel filtration on sephacryl s-200 and b ...	1998	9785347
propionibacterium acnes isolated from synovial tissue and fluid in a patient with oligoarthritis associated with acne and pustulosis.	this report describes the case of a patient with a 14-month course of severe oligoarthritis associated with acne. pure cultures of propionibacterium acnes were isolated from synovial tissue and synovial fluid specimens collected from the same joint after a 4-month interval. after 2 months of treatment with roxithromycin 300 mg/day, rifampicin 1,200 mg/day, and a nonsteroidal antiinflammatory drug (nsaid), followed by 4 months of treatment with azithromycin 1 gm/week and an nsaid, the synovitis p ...	1998	9778232
establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cdna into human myelomonocytic kg-1 cells.	we genetically engineered human myelomonocytic kg-i cells by introducing cdna of murine interleukin-18 receptor (muil-18r) and established human cells which were capable of responding to muil-18. these cells expressed larger number of muil-18r (> 13,000 sites/cell) than intrinsic human il-18 receptor (huil-18r) (< 2,500 sites/cell). and the cells responded to muil-18 as well as to huil-18 in a dose-dependent manner, and produced large amounts of interferon-gamma (ifn-gamma). we could estimate th ...	1998	9776579
effect of vaccination and the immunomodulators "bacillus of calmette-guérin, avridine and propionibacterium acnes" on rabies in mice.	responses of vaccination and treatment to immunomodulators against rabies in mice were evaluated through macrophage inhibition factor (mif), intra-pad inoculation (ipi) and serum neutralization (sn) tests and by the detection of gamma-interferon (ifn-gamma). onco-bcg, avridine and propionibacterium acnes were administered to groups of mice. higher survival rates were found in animals treated with p. acnes. lower levels of ifn-gamma were observed in the groups of infected and vaccinated mice. the ...	1998	9775360
[effect of silymarin on mouse liver damage, production and activity of tumor necrosis factor].	tumor necrosis factor (tnf) has been well-characterized as a prominent mediator in the development of liver injury. effects of silymarin (sb) on mouse liver damage, tnf production and activity were studied. pretreatment with sb (25-50 mg.kg-1, i.p., bid x 3 d) before the lipopolysaccharides (lps) injection markedly alleviated liver injury and diminished lps-induced tnf production in propionibacterium acnes (pa)-primed mice. sb (12.5-50 micrograms.ml-1) significantly inhibited lps-induced tnf rel ...	1996	9772703
stabilization of radical intermediates by an active-site tyrosine residue in methylmalonyl-coa mutase.	the adenosylcobalamin-dependent methylmalonyl-coa mutase catalyzes the reversible rearrangement of methylmalonyl-coa into succinyl-coa by a free-radical mechanism. the recently solved x-ray crystal structure of methylmalonyl-coa mutase from propionibacterium shermanii has shown that tyrosine 89 is an active-site residue involved in substrate binding. the role of tyrosine 89, a conserved residue among methylmalonyl-coa mutases, has been investigated by using site-directed mutagenesis to replace t ...	1998	9772164
mechanistic and structural studies on methylmalonyl-coa mutase.		1998	9765867
a rapid, enzymatic assay for the measurement of inorganic pyrophosphate in animal tissues.	a simple, rapid enzymatic assay for the determination of inorganic pyrophosphate in tissue and plasma has been developed using the enzyme pyrophosphate--fructose-6-phosphate 1-phosphotransferase (ec 2.7.1.90) which was purified from extracts of propionibacterium shermanii. the enzyme phosphorylates fructose-6-phosphate to produce fructose-1,6-bisphosphate using inorganic pyrophosphate as the phosphate donor. the utilization of inorganic pyrophosphate is measured by coupling the production of fru ...	1978	9762143
systematic review of propionibacterium acnes resistance to systemic antibiotics.	to document changes in the prevalence of resistance of propionibacterium acnes to antibiotics used for treating acne.	1998	9762064
production of a mixture of antimicrobial organic acids from lactose by co-culture of bifidobacterium longum and propionibacterium freudenreichii.	the antimicrobial activities of standard solutions of three organic acids (lactic, acetic, and propionic acids) were compared using micrococcus luteus, pseudomonas sp. and staphylococcus aureus as test microorganisms. at the same concentrations of the undissociated form, the antimicrobial activities of acetic and propionic acids were higher than that of lactic acid, irrespective of test microorganisms. in a single cultivation of bifidobacterium longum, a mixture of lactic (17 g/l) and acetic (20 ...	1998	9757558
update on acne.	acne is a common condition seen routinely by both primary care physicians and dermatologists. most patients have no underlying pathology and respond to traditional treatment; others, however, require more individualized evaluation and aggressive therapy. new information regarding the pathogenesis and treatment of acne is now available. this update discusses the proper evaluation of early childhood acne, the emergence of propionibacterium acnes resistance, and the rare but serious side effects oc ...	1998	9757362
mechanism of suppressed neutrophil mobilization in a mouse model for binge drinking: role of glucocorticoids.	the goals of this study were to determine if suppression of neutrophil accumulation and tnf-alpha production in the peritoneal cavity occurs in mice exposed to a chemical stressor [ethanol (etoh)], to evaluate the role of etoh-induced increases in endogenous glucocorticoids in any such suppression, and to determine if decreased tumor necrosis factor-alpha (tnf-alpha) production is responsible for decreases in neutrophil accumulation in etoh-treated mice. an inflammatory response induced in the p ...	1998	9756533
propionibacterium [correction of proprionibacterium] acnes infections of cerebrospinal fluid shunts.	ventricular cerebrospinal fluid shunt infections with propionibacterium acnes are generally low-grade, indolent infections. typical presentations include gradual shunt malfunction, nausea, headache, malaise, and infrequently, fever. in all, 489 shunt procedures performed between january 1992 and december 1995, and in 15 of these cases p. acnes was subsequently cultured from reservoir taps or an intraoperative culture which was obtained when the existing shunt components were revised. six of thes ...	1998	9753404
surface enhanced raman spectroscopic monitor of p. acnes lipid hydrolysis in vitro.	surface enhanced raman spectroscopy (sers) at a silver microelectrode was used to monitor bacterial hydrolysis of triglycerides in lipid mixtures that model sebaceous gland secretions. mixtures of wax esters, squalene, triolein, and triisostearin were used as model skin secretions. the transformation was followed in vitro as changes in the sers caused by hydrolysis of triglyceride to fatty acid. the fatty acid was adsorbed as its carboxylate, which is readily identified by the characteristic ban ...	1998	9741703
isolation and characterisation of obligately anaerobic, lipolytic bacteria from the rumen of red deer.	two gram-positive, obligately anaerobic, lipolytic bacteria, isolates lip4 and lip5, were obtained from the rumen contents of juvenile red deer. these mesophilic bacterial strains were capable of hydrolysing the neutral lipids, tallow, tripalmitin and oliver oil, into their constituent free long-chain fatty acid and glycerol moieties. the latter compound was dissimilated by both isolates, with isolate lip4 producing propionate as the predominant product, while isolate lip5 produced acetate, etha ...	1998	9741118
how a protein prepares for b12 binding: structure and dynamics of the b12-binding subunit of glutamate mutase from clostridium tetanomorphum.	glutamate mutase is an adenosylcobamide (coenzyme b12) dependent enzyme that catalyzes the reversible rearrangement of (2s)-glutamate to (2s,3s)-3-methylaspartate. the enzyme from clostridium tetanomorphum comprises two subunits (of 53.7 and 14.8 kda) and in its active form appears to be an alpha 2 beta 2 tetramer. the smaller subunit, termed muts, has been characterized as the b12-binding component. knowledge on the structure of a b12-binding apoenzyme does not exist.	1998	9739092
right ventricular endocarditis due to infection of pacemaker apparatus.	this article describes an unusual case of endocarditis in a 73-year-old man with a permanent pacemaker. echocardiography revealed vegetations in the right ventricle below the tricuspid valve only, and he required open heart surgery for removal of the pacemaker system. this case demonstrates the potentially protracted course of infective endocarditis many years after pacemaker insertion. it also emphasizes the importance of echocardiography in diagnosing right ventricular vegetations; and the nee ...	1998	9735684
superficial suppurative thrombophlebitis in children, caused by anaerobic bacteria.	the purpose of this study was to present the microbiology and clinical features of six children with superficial suppurative thrombophlebitis (sst) caused by anaerobic bacteria.	1998	9722004
preliminary characterization of microflora of comté cheese.	the evolution of the microflora of three comté cheeses made in duplicate with raw milk from three different sources was followed during ripening. the same starter was used with each type of milk. the comparison of the cheeses did not reveal any significant difference in the development of the microflora. starter lactic acid bacteria (streptococcus thermophilus and lactobacillus helveticus), which are added at the beginning of manufacture, decreased quickly in the first stages of ripening support ...	1998	9721662
production of conjugated linoleic acid by dairy starter cultures.	nineteen different strains of lactobacilli, lactococci, streptococci and propionibacteria commonly used as dairy starter cultures were tested for their ability to produce conjugated linoleic acid (cla) from free linoleic acid in vitro. two strains of propionibacterium freudenreichii ssp. freudenreichii and one strain of p. freudenreichii ssp. sheramnii were found to be capable of converting free linoleic acid to extracellular cla. the highest level of cla formed in the media was 265 micrograms m ...	1998	9721660
[chronic postoperative endophthalmitis caused by propionibacterium acnes].	there is a report of three cases of chronic postoperative endophthalmitis following cataract operations. in two patients there was proven propionibacterium acnes. there is presented a technique to obtain specimens for microbiological examination and an overview of clinical experience in treatment of the mentioned less common types of endophthalmitis. early surgical treatment as well as intraocular injection of vancomycin can help to restore a good visual acuity despite of longlasting inflammatio ...	1998	9721491
in vitro activity of hmr 3647 against anaerobic bacteria.	the aim of the present investigation was to determine the in vitro activity of hmr 3647 compared with other antimicrobial agents against anaerobic bacteria. the activity of hmr 3647 was determined against 342 clinical isolates of anaerobic bacteria by the agar dilution method and was compared with azithromycin, clarithromycin, roxithromycin, erythromycin, cefoxitin, imipenem, clindamycin and metronidazole. among the macrolides hmr 3647 and among the beta-lactams imipenem were the most active age ...	1998	9720465
hepatoprotective effect of propagermanium on corynebacterium parvum and lipopolysaccharide-induced liver injury in mice.	propagermanium is an organic germanium compound with immunopotentiating activity. we examined the hepatoprotective effect of propagermanium and its mechanism in an experimental animal model of acute liver injury induced with corynebacterium parvum (c. parvum) and lipopolysaccharide (lps) injection. oral pretreatment with propagermanium decreased alanine aminotransferase (alt) and aspartate aminotransferase (ast) activity in a dose-dependent manner. significant attenuation of alt and ast activity ...	1998	9716110
study of the morphology of the cell walls of some strains of lactic acid bacteria and related species.	the objective of the present study was to find an explanation for the biological effect of the bacteria present in a biotherapeutic milk (lactobacillus casei crl 431 and lactobacillus acidophilus crl 730). the ability of bacterial cell walls to induce an immune response when introduced into an organism is well known. in this paper we specifically analyzed the morphology of these cell walls. besides the two bacterial strains used in the fermented milk, two other lactic acid bacteria, belonging to ...	1998	9709227
response of cultures of propionibacterium to acid and low ph: tolerance and inhibition.	seventeen propionibacterium strains were tested for acid production and final ph achieved on glucose, fructose, or maltose as the primary carbon source. on average, strains of propionibacterium acidipropionici produced more acid and reached lower final ph values than did strains of the other species. three strains of p. acidipropionici, one propionibacterium jensenii, and two propionibacterium thoenii strains were tested further for the ability to survive and/or grow at low ph with lactic, hydro ...	1998	9708284
classification and identification of propionibacteria based on ribosomal rna genes and pcr.	a rapid method was developed to differentiate the genus propionibacterium from other genera by using a modified multiplex-pcr (mpcr) approach. three 16s rrna-targeted oligonucleotide primers were designed to amplify simultaneously two dna-fragments in the mpcr assay. the universal primer pair bak11w and bak4 (corresponding to the e. coli 16s rrna positions 8-25 and 1522-1540, respectively) was used in combination with the primer pair bak4 and gd1 (5'-tgctttcgatacgggttgac-3'). the later sequence ...	1998	9704111
[bacterial colonization of conjunctiva with propionibacterium acnes before and after polyvidon iodine administration before intraocular interventions].	propionibacterium acnes has been described as a causative agent of postoperative endophthalmitis. this gram-positive, immotile, non-spore-forming bacterium is highly pleomorphic and grows under conditions of low to no oxygen concentration. it is commonly found on the skin at the openings of sebaceous glands and on hairs. a near-symptomless postoperative endophthalmitis occurs particularly when propionibacteria are enclosed in the capsular bag. we investigated to what extent the number of p. acne ...	1998	9703725
improved detection of infection in hip replacements. a currently underestimated problem.	our aim was to determine if the detection rate of infection of total hip replacements could be improved by examining the removed prostheses. immediate transfer of prostheses to an anaerobic atmosphere, followed by mild ultrasonication to dislodge adherent bacteria, resulted in the culture of quantifiable numbers of bacteria, from 26 of the 120 implants examined. the same bacterial species were cultured by routine microbiological techniques from only five corresponding tissue samples. tissue remo ...	1998	9699813
pancreatic fluid collections: diagnostic and therapeutic implications of percutaneous drainage guided by ultrasound.	the aim of the present study is to assess the usefulness of biochemical and bacteriological analysis of the pancreatic fluid obtained at percutaneous drainage of pancreatic pseudocysts and abscesses guided by ultrasound.	1998	9684149
energy conservation in the decarboxylation of dicarboxylic acids by fermenting bacteria.	decarboxylation of dicarboxylic acids (oxalate, malonate, succinate, glutarate, and malate) can serve as the sole energy source for the growth of fermenting bacteria. since the free energy change of a decarboxylation reaction is small (around -20 kj per mol) and equivalent to only approximately one-third of the energy required for atp synthesis from adp and phosphate under physiological conditions, the decarboxylation energy cannot be conserved by substrate-level phosphorylation. it is either co ...	1998	9683642
percutaneous suction aspiration and drainage for pyogenic spondylitis.	retrospective evaluation of results in 23 cases of early-stage pyogenic spondylitis treated with percutaneous suction aspiration and drainage.	1998	9682317
comparative in vitro activity of bay 12-8039 and five other antimicrobial agents against anaerobic bacteria.	the in vitro activity of bay 12-8039 against 360 anaerobic clinical isolates was determined by the agar dilution method and compared to that of five other antimicrobial agents. bay 12-8039 and imipenem were the most active agents tested. the following mic90 values were determined for bay 12-8039: peptostreptococcus spp. (50 isolates), 1 mg/l; propionibacterium acnes (30 isolates). 0.25 mg/l; clostridium perfringens (30 isolates), 0.5 mg/l; clostridium difficile (50 isolates), 2 mg/l; bacteroides ...	1998	9665302
16s rrna mutation associated with tetracycline resistance in a gram-positive bacterium.	a genetic basis for tetracycline resistance in cutaneous propionibacteria was suggested by comparing the nucleotide sequences of the 16s rrna genes from 16 susceptible and 21 resistant clinical isolates and 6 laboratory-selected tetracycline-resistant mutants of a susceptible strain. fifteen clinical isolates resistant to tetracycline were found to have cytosine instead of guanine at a position cognate with escherichia coli 16s rrna base 1058 in a region important for peptide chain termination a ...	1998	9661007
subgingival microflora associated with nifedipine-induced gingival overgrowth.	the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ...	1998	9660335
immunologic function in horses after non-specific immunostimulant administration.	inactivated propionibacterium acnes is a biologic response modifier for treatment of non-specific respiratory disease in horses. the objectives of this investigation were to determine alterations in phagocytic activity, phenotypic expression of lymphocyte subpopulations and lymphokine-activated killing cell response in healthy young horses. samples were collected on day 0, 7 and 14 of the investigation. blood samples were obtained via jugular venipuncture and pulmonary leukocytes were recovered ...	1998	9656421
conformational changes on substrate binding to methylmalonyl coa mutase and new insights into the free radical mechanism.	methylmalonyl coa mutase catalyses the interconversion of succinyl coa and methylmalonyl coa via a free radical mechanism. the enzyme belongs to a family of enzymes that catalyse intramolecular rearrangement reactions in which a group and a hydrogen atom on adjacent carbons are exchanged. these enzymes use the cofactor adenosylcobalamin (coenzyme b12) which breaks to form an adenosyl radical, thus initiating the reaction. determination of the structure of substrate-free methylmalonyl coa mutase ...	1998	9655823
microbiology of cervical lymphadenitis in adults.	the microbiology of needle aspirates from 40 inflamed cervical lymph glands was studied for aerobic and anaerobic bacteria, fungi and mycobacteria. forty-two bacterial, 11 mycobacterial and six fungal isolates were isolated. aerobic bacteria only were recovered in 11 (27.5%), anaerobes alone in five (12.5%) and mixed aerobic and anaerobic bacteria in seven (17.5%). mycobacterium sp. were recovered in 11 (27.5%) and fungi in six (15%). the recovery of anaerobes was associated with dental infectio ...	1998	9655225
adjuvant properties of killed propionibacterium avidum kp-40 in vaccination of dogs against canine parvovirosis.	immunomodulating and adjuvant properties of propionibacterium avidum kp-40 (pa), a potent stimulator of the macrophage-monocyte system and inducer of endogenous interferon, were tested in healthy dogs and in dogs vaccinated against canine parvovirosis (cpv). a single subcutaneous injection of pa (0.5 mg/kg b. m.) was administered either 10 days before or simultaneously with cpv immunization. the immunomodulating properties of pa were expressed by enhancement of phagocytic and bactericidal activi ...	1998	9646552