Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| production of bacteriocin-like inhibitory substance by bifidobacterium lactis in skim milk supplemented with additives. | bacteriocins are natural compounds used as food biopreservatives instead of chemical preservatives. bifidobacterium animalis subsp. lactis (bifid. lactis) was shown to produce a bacteriocin-like inhibitory substance (blis) able to inhibit the growth of listeria monocytogenes selected as an indicator microorganism. to enhance this production by the strain bifid. lactis bl 04, skim milk (sm) was used as a fermentation medium either in the presence or in the absence of yeast extract, tween 80 or in ... | 2015 | 25850555 |
| odor fingerprinting of listeria monocytogenes recognized by spme-gc-ms and e-nose. | microorganisms can produce species-specific microbial volatile organic compounds (mvocs), or odor compounds, which can be characterized by odor fingerprinting. the objective of this study was to characterize the odor fingerprint of listeria monocytogenes. solid-phase microextraction-gas chromatography-mass spectrometry (spme-gc-ms) and electronic nose (e-nose) were used to recognize the mvocs of l. monocytogenes in pure culture medium. the main mvocs of l. monocytogenes were identified by spme-g ... | 2015 | 25847596 |
| stochastic modelling of listeria monocytogenes single cell growth in cottage cheese with mesophilic lactic acid bacteria from aroma producing cultures. | a stochastic model was developed for simultaneous growth of low numbers of listeria monocytogenes and populations of lactic acid bacteria from the aroma producing cultures applied in cottage cheese. during more than two years, different batches of cottage cheese with aroma culture were analysed for ph, lactic acid concentration and initial concentration of lactic acid bacteria. these data and bootstrap sampling were used to represent product variability in the stochastic model. lag time data wer ... | 2015 | 25847186 |
| effect of filling type and heating method on prevalence of listeria species and listeria monocytogenes in dumplings produced in poland. | the count of listeria monocytogenes was determined, before and after heat treatment, in 200 samples of dumplings of 9 brands and with different types of stuffing. analyses were conducted according to iso 11290-1 standard and with real-time pcr method. the highest count of l. monocytogenes was found in meat dumplings (10(2) to 10(4) cfu/g), whereas products with white cheese-potato stuffing and vegetable-mushroom stuffing contained significantly less listeria, 20 to 80 and 5 to 32 cfu/g, respecti ... | 2015 | 25847074 |
| effect of sulfur dioxide fumigation on survival of foodborne pathogens on table grapes under standard storage temperature. | we examined the fate of listeria monocytogenes, escherichia coli o157:h7, and salmonella enterica thompson inoculated on freshly-harvested table grapes under standard cold storage with initial and weekly sulfur dioxide (so2) fumigation. l. monocytogenes and s. enterica thompson were much more sensitive to cold temperature than e. coli o157:h7. furthermore, l. monocytogenes was highly susceptible to so2. initial fumigation with 100 or 200 ppm-hr was sufficient to eliminate this pathogen on grapes ... | 2015 | 25846930 |
| assessment of microbial risk factors and impact of meteorological conditions during production of baby spinach in the southeast of spain. | there is a timely need to evaluate the effect agricultural factors and meteorological conditions on fresh produce contamination. this study evaluated those risk factors and described, for the first time, the distribution of indicator microorganisms (escherichia coli, enterococcus, coliforms, and enterobacteriaceae) and the prevalence of foodborne pathogens (enterohaemorrhagic e. coli, listeria monocytogenes and salmonella spp.) in baby spinach grown in the southeast of spain. a longitudinal stud ... | 2015 | 25846928 |
| effect of ripeness stage during processing on listeria monocytogenes growth on fresh-cut 'conference' pears. | there are several factors that affect the shelf life of fresh-cut fruit, including the cultivar, the ripeness stage of the fruit during processing and the fruit's storage atmosphere and temperature. the effect of fruit ripeness during processing on the survival and growth of listeria monocytogenes on fresh-cut 'conference' pear slices at different temperatures (5, 10 and 20 °c) was studied. the four ripeness stages studied in this work (assessed by a fruit's firmness) were mature-green (54-60 n) ... | 2015 | 25846921 |
| microbial biofilms in seafood: a food-hygiene challenge. | seafood forms a part of a healthy diet. however, seafood can be contaminated with foodborne pathogens, resulting in disease outbreaks. because people consume large amounts of seafood, such disease outbreaks are increasing worldwide. seafood contamination is largely due to the naturally occurring phenomenon of biofilm formation. the common seafood bacterial pathogens that form biofilms are vibrio spp., aeromonas hydrophila, salmonella spp., and listeria monocytogenes. as these organisms pose a gl ... | 2015 | 25846914 |
| management of listeria monocytogenes in fermented sausages using the food safety objective concept underpinned by stochastic modeling and meta-analysis. | in the present work, a demonstration is made on how the risk from the presence of listeria monocytogenes in fermented sausages can be managed using the concept of food safety objective (fso) aided by stochastic modeling (bayesian analysis and monte carlo simulation) and meta-analysis. for this purpose, the icmsf equation was used, which combines the initial level (h0) of the hazard and its subsequent reduction (σr) and/or increase (σi) along the production chain. each element of the equation was ... | 2015 | 25846913 |
| predicting pathogen-specific cd8 t cell immune responses from a modeling approach. | the primary cd8 t cell immune response constitutes a major mechanism to fight an infection by intra-cellular pathogens. we aim at assessing whether pathogen-specific dynamical parameters of the cd8 t cell response can be identified, based on measurements of cd8 t cell counts, using a modeling approach. we generated experimental data consisting in cd8 t cell counts kinetics during the response to three different live intra-cellular pathogens: two viruses (influenza, vaccinia) injected intranasall ... | 2015 | 25846273 |
| effects of sub-lethal concentrations of thyme and oregano essential oils, carvacrol, thymol, citral and trans-2-hexenal on membrane fatty acid composition and volatile molecule profile of listeria monocytogenes, escherichia coli and salmonella enteritidis. | the aim of this work was to investigate the modifications of cell membrane fatty acid composition and volatile molecule profiles of listeria monocytogenes, salmonella enteritidis, escherichia coli, during growth in the presence of different sub-lethal concentrations of thyme and oregano essential oils as well as carvacrol, thymol, trans-2-hexenal and citral. the results evidenced that the tested molecules induced noticeable modifications of membrane fatty acid profiles and volatile compounds pro ... | 2015 | 25842326 |
| two-component-system histidine kinases involved in growth of listeria monocytogenes egd-e at low temperatures. | two-component systems (tcss) aid bacteria in adapting to a wide variety of stress conditions. while the role of tcs response regulators in the cold tolerance of the psychrotrophic foodborne pathogen listeria monocytogenes has been demonstrated previously, no comprehensive studies showing the role of tcs histidine kinases of l. monocytogenes at low temperature have been performed. we compared the expression levels of each histidine kinase-encoding gene of l. monocytogenes egd-e in logarithmic gro ... | 2015 | 25841007 |
| purification and characterization of a bacteriocin from an oenological strain of leuconostoc mesenteroides subsp. cremoris. | malolactic fermentation (mlf), which improves organoleptic properties and biologic stability of some wines, may cause wine spoilage if uncontrolled. bacteriocins were reported as efficient preservatives to control mlf through their bactericidal effect on malolactic bacteria. leuconostoc mesenteroides subsp. cremoris w3 isolated from wine produces an inhibitory substance that is bactericidal against malolactic bacteria in model wine medium. treatment of the culture supernatant of strain w3 with p ... | 2016 | 25837975 |
| aerobic plate counts and atp levels correlate with listeria monocytogenes detection in retail delis. | listeria monocytogenes is a foodborne pathogen that causes an estimated 1,591 cases of illness and 255 deaths annually in the united states, the majority of which are attributed to ready-to-eat deli meats processed in retail delis. because retail delis distribute product directly to consumers, rapid methods to validate cleaning and sanitation are needed to improve retail food safety. this study investigated the relationships among atp levels, standard aerobic plate count (apc), and l. monocytoge ... | 2015 | 25836412 |
| growth inhibition of listeria monocytogenes, salmonella enterica, and escherichia coli o157:h7 by d-tryptophan as an incompatible solute. | under osmotic stress, bacterial cells uptake compatible solutes such as glycine-betaine to maintain homeostasis. it is unknown whether incompatible solutes exist that are similar in structure to compatible solutes but have adverse physiological effects on bacterial physiology. the objective of this study was to evaluate solute incompatibility of various amino acids against bacterial growth. bacterial growth was evaluated by changes in optical density at 595 nm in peptone-yeast-glucose (pyg) brot ... | 2015 | 25836411 |
| microbiological diversity and prevalence of spoilage and pathogenic bacteria in commercial fermented alcoholic beverages (beer, fruit wine, refined rice wine, and yakju). | the present study examined 469 commercially available fermented alcoholic beverages (fabs), including beer (draft, microbrewed, and pasteurized), fruit wine (grape and others), refined rice wine, and yakju (raw and pasteurized). samples were screened for escherichia coli and eight foodborne pathogens (bacillus cereus, campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella spp., staphylococcus aureus, and yersinia enterocolitica), and the aerob ... | 2015 | 25836410 |
| analysis of older adults' domestic kitchen storage practices in the united kingdom: identification of risk factors associated with listeriosis. | increased listeriosis incidence among older adults (≥ 60 years) has been reported internationally, with many cases reported to be sporadic and associated with ready-to-eat (rte) food products with extended refrigerated shelf life. given that the home kitchen is recognized as a significant location where foodborne illnesses are acquired, it is important that consumers implement safe food practices to minimize risks. this is crucial for vulnerable consumers, such as older adults. consumer food saf ... | 2015 | 25836399 |
| effectiveness of sanitizing products on controlling selected pathogen surrogates on retail deli slicers. | the objectives of this study were (i) to assess the efficacy of quaternary ammonium chloride-based wet foam (wf) and dry foam (df) sanitizer systems (600 ppm) for reducing listeria innocua (a nonpathogenic surrogate of listeria monocytogenes) or a 100.0 μg/ml rifampin-resistant salmonella typhimurium lt2 (a nonpathogenic surrogate of salmonella enterica serovar typhimurium) on niche and transfer point areas of an unwashed retail deli slicer as compared with traditional chlorine (cl(-)) treatment ... | 2015 | 25836395 |
| evaluation of novel micronized encapsulated essential oil-containing phosphate and lactate blends for growth inhibition of listeria monocytogenes and salmonella on poultry bologna, pork ham, and roast beef ready-to-eat deli loaves. | essential oils and their constituents are reported to possess potent antimicrobial activity, but their use in food processing is limited because of low solubility in aqueous systems and volatilization during processing. two proprietary noncommercial essential oil-containing phosphate blends were evaluated for antimicrobial activity against salmonella enterica cocktail (sc)-and listeria monocytogenes (lm)-inoculated deli meat products made from pork, poultry, or beef. four treatments were tested ... | 2015 | 25836394 |
| effect of oxygen stress on growth and survival of clostridium perfringens, campylobacter jejuni, and listeria monocytogenes under different storage conditions. | this study investigated the growth and survival of three foodborne pathogens (clostridium perfringens, campylobacter jejuni, and listeria monocytogenes) in beef (7% fat) and nutrient broth under different oxygen levels. samples were tested under anoxic (<0.5%), microoxic (6 to 8%), and oxic (20%) conditions during storage at 7 °c for 14 days and at 22 °c for 5 days. two initial inoculum concentrations were used (1 and 2 log cfu per g of beef or per ml of broth). the results show that c. perfring ... | 2015 | 25836393 |
| determining the potential link between irrigation water quality and the microbiological quality of onions by phenotypic and genotypic characterization of escherichia coli isolates. | the potential transfer of human pathogenic bacteria present in irrigation water onto fresh produce was investigated, because surface water sources used for irrigation purposes in south africa have increasingly been reported to be contaminated with enteric bacterial pathogens. a microbiological analysis was performed of a selected river in limpopo province, south africa, that is often contaminated with raw sewage from municipal sewage works and overhead irrigated onions produced on a commercial f ... | 2015 | 25836387 |
| expert elicitation as a means to attribute 28 enteric pathogens to foodborne, waterborne, animal contact, and person-to-person transmission routes in canada. | enteric illness contributes to a significant burden of illness in canada and globally. understanding its sources is a critical step in identifying and preventing health risks. expert elicitation is a powerful tool, used previously, to obtain information about enteric illness source attribution where information is difficult or expensive to obtain. thirty-one experts estimated transmission of 28 pathogens via major transmission routes (foodborne, waterborne, animal contact, person-to-person, and ... | 2015 | 25835810 |
| serotypes and pulsotypes diversity of listeria monocytogenes in a beef-processing environment. | utensils and equipment from meat-processing facilities are considered relevant cross-contamination points of listeria monocytogenes to foods, demanding tracking studies to identify their specific origins, and predict proper control. the present study aimed to detect l. monocytogenes in a beef-processing facility, investigating the diversity of serotypes and pulsotypes in order to identify the possible contamination routes. surface samples from knives (n=26), tables (n=78), and employees hands (n ... | 2015 | 25835809 |
| efficacy of cell wall-deficient spheroplasts against experimental murine listeriosis. | various strategies adapted to develop an efficient vaccine against foodborne pathogen, listeria monocytogenes, have met with little success. spheroplasts (bacterial cell devoid of cell wall) are likely to undergo membrane-membrane fusion, leading to the delivery of their content to the cytosol of antigen-presenting cells, thus facilitating mhc class i antigen processing and presentation. in this study, we evaluated the prophylactic potential of listeria spheroplast-based vaccine against experime ... | 2015 | 25833403 |
| antimicrobial activities of active component isolated from lawsonia inermis leaves and structure-activity relationships of its analogues against food-borne bacteria. | the antimicrobial activities of lawsonia inermis leaf extract and 2-hydroxy-1,4-naphthoquinone analogues against food-borne bacteria. the antimicrobial activities of five fractions derived from the methanol extract of lawsonia inermis leaves were evaluated against 7 food-borne bacteria. 2-hydroxy-1,4-naphthoquinone was isolated by chromatographic analyses. 2-hydroxy-1,4-naphthoquinone showed the strong activities against bacillus cereus, listeria monocytogenes, salmonella enterica, shigella sonn ... | 2015 | 25829631 |
| enhanced bactericidal effect of enterocin a in combination with thyme essential oils against l. monocytogenes and e. coli o157:h7. | the combined effects of enterocin a with thymus vulgaris essential oils (eos) against listeria monocytogenes and escherichia coli o157:h7 were investigated in vitro by enumeration of surviving populations of testing pathogens and minimal inhibitory concentration (mic) determination. enterocin a was purified to homogeneity by rp-hplc from the culture fluid of enterococcus strain and thyme eos were extracted from local thymus vulgaris plants. the major constituent of thyme eos oils determined by g ... | 2013 | 25829595 |
| colovesical fistula presenting as listeria monocytogenes bacteraemia. | we present a case of colovesical fistula presenting with a clinical syndrome of urosepsis subsequently demonstrated to be due to listeria monocytogenes bacteraemia. the patient had a history of previous rectal cancer with a low anterior resection and a covering ileostomy that had been reversed 6 months prior to this presentation. l. monocytogenes was also isolated among mixed enteric organisms on urine culture. there were no symptoms or signs of acute gastrointestinal listeriosis or meningoencep ... | 2015 | 25827919 |
| genetic characteristics of japanese clinical listeria monocytogenes isolates. | listeria monocytogenes causes foodborne illnesses through consumption of ready-to-eat foods. although 135-201annual listeriosis cases have been estimated in japan, the details regarding the clinical isolates such as infection source, virulence level, and other genetic characteristics, are not known. in order to uncover the trends of listeriosis in japan and use the knowledge for prevention measures to be taken, the genetic characteristics of the past human clinical isolates needs to be elucidate ... | 2015 | 25826318 |
| bacteriophage predation promotes serovar diversification in listeria monocytogenes. | listeria monocytogenes is a bacterial pathogen classified into distinct serovars (svs) based on somatic and flagellar antigens. to correlate phenotype with genetic variation, we analyzed the wall teichoic acid (wta) glycosylation genes of sv 1/2, 3 and 7 strains, which differ in decoration of the ribitol-phosphate backbone with n-acetylglucosamine (glcnac) and/or rhamnose. inactivation of lmo1080 or the dtdp-l-rhamnose biosynthesis genes rmlacbd (lmo1081-1084) resulted in loss of rhamnose, where ... | 2015 | 25825127 |
| listeria monocytogenes spreads within the brain by actin-based intra-axonal migration. | listeria monocytogenes rhombencephalitis is a severe progressive disease despite a swift intrathecal immune response. based on previous observations, we hypothesized that the disease progresses by intra-axonal spread within the central nervous system. to test this hypothesis, neuroanatomical mapping of lesions, immunofluorescence analysis, and electron microscopy were performed on brains of ruminants with naturally occurring rhombencephalitis. in addition, infection assays were performed in bovi ... | 2015 | 25824833 |
| the human p-glycoprotein transporter enhances the type i interferon response to listeria monocytogenes infection. | human multidrug efflux transporters are known for their ability to extrude antibiotics and toxic compounds out of cells, yet accumulating data indicate they have additional functions in diverse physiological processes not related to drug efflux. here, we show that the human multidrug transporter p-glycoprotein (p-gp) (also named mdr1 and abcb1) is transcriptionally induced in the monocytic cell line thp-1 upon infection with the human intracellular bacterial pathogen listeria monocytogenes. nota ... | 2015 | 25824830 |
| identification of a peptide-pheromone that enhances listeria monocytogenes escape from host cell vacuoles. | listeria monocytogenes is a gram-positive facultative intracellular bacterial pathogen that invades mammalian cells and escapes from membrane-bound vacuoles to replicate within the host cell cytosol. gene products required for intracellular bacterial growth and bacterial spread to adjacent cells are regulated by a transcriptional activator known as prfa. prfa becomes activated following l. monocytogenes entry into host cells, however the signal that stimulates prfa activation has not yet been de ... | 2015 | 25822753 |
| bacillus subtilis subsp. subtilis cbmdc3f with antimicrobial activity against gram-positive foodborne pathogenic bacteria: uv-maldi-tof ms analysis of its bioactive compounds. | in this work a new bacillus sp. strain, isolated from honey, was characterized phylogenetically. its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (uv-maldi ms). bacillus cbmdc3f was phylogenetically characterized as bacillus subtilis subsp. subtilis after rrna analysis of the 16s subunit and the gyra gene (access codes genbank jx ... | 2015 | 25820813 |
| an update on the transport and metabolism of iron in listeria monocytogenes: the role of proteins involved in pathogenicity. | listeria monocytogenes is a gram-positive bacterium that causes a rare but severe human disease with high mortality rate. the microorganism is widespread in the natural environment where it shows a saprophytic lifestyle. in the human body it infects many different cell types, where it lives intracellularly, however it may also temporarily live extracellularly. the ability to survive and grow in such diverse niches suggests that this bacterium has a wide range of mechanisms for both the acquisiti ... | 2015 | 25820385 |
| comparison of the prevalences and diversities of listeria species and listeria monocytogenes in an urban and a rural agricultural watershed. | foods and related processing environments are commonly contaminated with the pathogenic listeria monocytogenes. to investigate potential environmental reservoirs of listeria spp. and l. monocytogenes, surface water and point source pollution samples from an urban and a rural municipal water supply watershed in nova scotia, canada, were examined over 18 months. presumptive listeria spp. were cultured from 72 and 35% of rural and urban water samples, respectively, with 24% of the positive samples ... | 2015 | 25819965 |
| the listeria monocytogenes strain 10403s biocyc database. | listeria monocytogenes is a food-borne pathogen of humans and other animals. the striking ability to survive several stresses usually used for food preservation makes l. monocytogenes one of the biggest concerns to the food industry, while the high mortality of listeriosis in specific groups of humans makes it a great concern for public health. previous studies have shown that a regulatory network involving alternative sigma (σ) factors and transcription factors is pivotal to stress survival. ho ... | 2015 | 25819074 |
| tumor necrosis factor-α, interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in ossimi sheep with encephalitic listeriosis. | encephalitic listeriosis in sheep is a life-threatening disease. however, little is known about the cytokine response and their predictive value in this disease. the aim of present study was to assess the prognostic significance of tumor necrosis factor-α (tnf-α), interleukin-12(p40) (il-12 p40), interleukin-6 (il-6), and interleukin 10 (il-10) levels in cerebrospinal fluid (csf) in sheep with encephalitic listeriosis. fifty-nine ewes in 14 flocks were diagnosed clinically as having listeriosis. ... | 2015 | 25817420 |
| [morphologic changes in listeria cells under the effect of metabolites of human intestinal microflora enterococci]. | characterization of morphologic changes in listeria cells under the effect of metabolites of human intestine microflora enterococci. | 2015 | 25816518 |
| ascorbic acid, ultraviolet c rays, and glucose but not hyperthermia are elicitors of human β-defensin 1 mrna in normal keratinocytes. | hosts' innate defense systems are upregulated by antimicrobial peptide elicitors (apes). our aim was to investigate the effects of hyperthermia, ultraviolet a rays (uva), and ultraviolet c rays (uvc) as well as glucose and ascorbic acid (aa) on the regulation of human β-defensin 1 (defb1), cathelicidin (camp), and interferon-γ (ifng) genes in normal human keratinocytes (nhk). the indirect in vitro antimicrobial activity against staphylococcus aureus and listeria monocytogenes of these potential ... | 2015 | 25815330 |
| a naturally occurring prfa truncation in a listeria monocytogenes field strain contributes to reduced replication and cell-to-cell spread. | listeria (l.) monocytogenes is an environmental bacterium that may become an intracellular pathogen upon ingestion to cause gastroenteritis, septicaemia, abortions, and/or fatal infections of the central nervous system. we here describe a l. monocytogenes field strain (jf5171) isolated from a bovine placenta in the context of abortion, which exhibited attenuation in bovine brain-slice cultures. the whole genome of strain jf5171 was sequenced, and the invasion, replication, and intercellular spre ... | 2015 | 25813546 |
| characterization of listeria monocytogenes from three countries and antibiotic resistance differences among countries and listeria monocytogenes serogroups. | a total of 104 listeria monocytogenes isolates from 330 fish samples from three countries were characterized by multiplex pcr for serogrouping and virulence markers determination and tested for antibiotics resistance. a 53·8% of the isolates belonged to serogroup 1/2a, 3a; 32% belonged to 1/2b, 3b, 7; 14·4% belonged to 4b, 4d, 4e and 1% belonged to 1/2c, 3c. all isolates exhibited resistance to at least one antibiotic but the resistance rates varied among countries. the isolates exhibited high r ... | 2015 | 25808878 |
| antimicrobial effects of essential oils, nisin, and irradiation treatments against listeria monocytogenes on ready-to-eat carrots. | the study aimed at using essential oil (eo) alone or combined eo with nisin and low dose γ-irradiation to evaluate their antibacterial effect against listeria monocytogenes during storage of carrots at 4 °c. minicarrots were inoculated with l. monocytogenes at a final concentration of approximately 7 log cfu/g. inoculated samples were coated by nisin at final concentration of 10(3) international unit (iu)/ml or individual mountain savory eo or carvacrol at final concentration of 0.35%, w/w) or n ... | 2015 | 25807882 |
| division of human listeria monocytogenes pulsed-field gel electrophoresis (pfge) types belonging to lineage i (serovar 4b, 1/2b, and 3b) into pfge groups. | the 63 pulsed-field gel electrophoresis (pfge) types identified among 427 clinical isolates of listeria monocytogenes that were characterized in a previous study by serotyping and pfge (asci) could be further divided into 17 pfge groups. while the 63 pfge types, all part of lineage i, were established based on the number and distribution of all bands in each dna profile, the 17 pfge groups were based on the configuration of small bands with sizes <145.5 kb. the 30 pfge types of l. monocytogenes ... | 2015 | 25803595 |
| pneumonia by listeria monocytogenes: a common infection by an uncommon pathogen. | infections by listeria monocytogenes typically occur in infants, the elderly, pregnant women, and immunosuppressed subjects. pulmonary infections in adults are extremely uncommon and only few reports can be found in the literature. we here report a case of listeria pneumonia in an 85-year-old female patient and we discuss our diagnostic and therapeutic approach. despite being rare and in most cases difficult to be identified, listeria pneumonia should always be considered in immunosuppressed pat ... | 2015 | 25802774 |
| fournier's gangrene caused by listeria monocytogenes as the primary organism. | a 70-year-old man with a history of tongue cancer presented with fournier's gangrene caused by listeria monocytogenes serotype 4b. surgical debridement revealed undiagnosed rectal adenocarcinoma. the patient did not have an apparent dietary or travel history but reported daily consumption of sashimi (raw fish). old age and immunodeficiency due to rectal adenocarcinoma may have supported the direct invasion of l monocytogenes from the tumour. the present article describes the first reported case ... | 2017 | 25798155 |
| molecular serotyping and pathogenic potential of listeria monocytogenes isolated from milk and milk products in tamil nadu, india. | listeria monocytogenes, an important bacterial pathogen, is responsible for foodborne illnesses worldwide. examination of food samples for the presence of l. monocytogenes and assessment of their pathogenicity is usually an effective strategy in the prevention of listeriosis. in the present study, we have tested 307 samples of milk and milk products from various places in tamil nadu, india for the presence of l. monocytogenes using iso 11290 and u.s. food and drug administration bacteriological ... | 2015 | 25793931 |
| characterization of indigenous lactobacillus strains for probiotic properties. | probiotics are defined as adequate amount of live microorganisms able to confer health benefits on the host. currently, most commercially available probiotic products in the market belong to genera lactobacillus. traditional dairy products are usually rich source of lactobacillus strains with significant health benefits. in order to evaluate the probiotic potential of these bacteria, it is essential to assess their health benefits, efficacy, and safety. | 2015 | 25793099 |
| complete genome sequence of the persistent listeria monocytogenes strain r479a. | the complete genome sequence of the persistent listeria monocytogenes strain r479a isolated from smoked salmon in denmark and belonging to lineage ii, serovar 1/2a, and multilocus sequence type 8 (st8) is presented here. | 2015 | 25792065 |
| sensitive enumeration of listeria monocytogenes and other listeria species in various naturally contaminated matrices using a membrane filtration method. | for the enumeration of listeria monocytogenes (l. monocytogenes) in food, a sensitive enumeration method has been recently developed. this method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate l. monocytogenes. an evaluation of this method was performed with several categories of foods naturally contaminated with l. monocytogenes. the results obtained with this technique were compared with those obtained from the mod ... | 2015 | 25791005 |
| bacteriocin production and inhibition of listeria monocytogenes by lactobacillus sakei subsp. sakei 2a in a potentially synbiotic cheese spread. | survival, bacteriocin(s) production, and antilisterial effect of lactobacillus sakei subsp. sakei 2a were evaluated in a potentially synbiotic cheese spread, throughout storage at 4 °c and 15 °c for up to 28 days, using culture-dependent (plate count) and culture-independent (qpcr) methods. bacteriocin(s) production in the food product was monitored by phenotypic and molecular (rt-qpcr) techniques. three cheese spread trials (t) containing the prebiotic fiber inulin were produced in duplicates a ... | 2015 | 25791002 |
| antilisterial activity of bacteriocinogenic pediococcus acidilactici ha6111-2 and lactobacillus plantarum esb 202 grown under ph and osmotic stress conditions. | bacteriocin producing lactic acid bacteria (lab) cultures can be used as biopreservatives in fermented food products; thus the food industry is interested in stable cultures that produce bacteriocins consistently. inhibtion of listeria spp. by bacteriocinogenic pediococcus acidilactici and lactobacillus plantarum (both isolated from fermented meats) was investigated under conditions of stress induced by low ph and high salt concentrations. listeria monocytogenes serogroup iib (from cheese), l. m ... | 2015 | 25790998 |
| a novel gene, lstc, of listeria monocytogenes is implicated in high salt tolerance. | listeria monocytogenes, causative agent of human listeriosis, has been isolated from a wide variety of foods including deli meats, soft cheeses, cantaloupes, sprouts and canned mushrooms. standard control measures for restricting microbial growth such as refrigeration and high salt are often inadequate as l. monocytogenes grows quite well in these environments. in an effort to better understand the genetic and physiological basis by which l. monocytogenes circumvents these controls, a transposon ... | 2015 | 25790994 |
| enhancing the antibacterial effect of 461 and 521 nm light emitting diodes on selected foodborne pathogens in trypticase soy broth by acidic and alkaline ph conditions. | light emitting diodes (leds) with their antibacterial effect present a novel method for food preservation. this effect may be influenced by environmental conditions such as the ph of the food contaminated by the pathogen. thus, it is necessary to investigate the influence of ph on the antibacterial effect of leds before their application to real food matrices. escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in trypticase soy broth were illuminated using 10-w 461 (22.1 ... | 2015 | 25790991 |
| effects of oxygen-depleted atmospheres on survival and growth of listeria monocytogenes on fresh-cut iceberg lettuce stored at mild abuse commercial temperatures. | the effects of oxygen-depleted atmospheres, 0.25% o2+12% co2 (balance n2) and 2% o2 + 6% co2 (balance n2), on growth of listeria monocytogenes on fresh-cut iceberg lettuce were determined. the study was carried out at mild abuse temperatures using controlled atmosphere chambers. during storage at a constant temperature of 7 °c, growth was enhanced at the lower oxygen level of 0.25% o2 by day 10. over 17 days of storage at temperatures designed to mimic mild abuse commercial conditions, there wer ... | 2015 | 25790986 |
| notes from the field: listeriosis associated with stone fruit--united states, 2014. | on july 19, 2014, a packing company in california (company a) voluntarily recalled certain lots of stone fruits, including whole peaches, nectarines, plums, and pluots, because of concern about contamination with listeria monocytogenes based on internal company testing. on july 31, the recall was expanded to cover all fruit packed at their facility during june 1-july 17. after the initial recall, clinicians, state and local health departments, cdc, and the food and drug administration (fda) rece ... | 2015 | 25789745 |
| combined effect of a nanoemulsion of d-limonene and nisin on listeria monocytogenes growth and viability in culture media and foods. | the present work evaluated the antibacterial effect of nanoemulsions from natural compounds d-limonene and nisin against listeria monocytogenes in tryptic soy broth growth medium, chicken broth, and vegetable cream. experiments were performed by means of optical density growth curves and studies of viability in culture media and foods. stability of nanoemulsions was evaluated by a mastersizer 2000 equipment. results showed greater effectiveness when applying d-limonene in form of nanoemulsion th ... | 2016 | 25788168 |
| hygienisation and nutrient conservation of sewage sludge or cattle manure by lactic acid fermentation. | manure from animal farms and sewage sludge contain pathogens and opportunistic organisms in various concentrations depending on the health of the herds and human sources. other than for the presence of pathogens, these waste substances are excellent nutrient sources and constitute a preferred organic fertilizer. however, because of the pathogens, the risks of infection of animals or humans increase with the indiscriminate use of manure, especially liquid manure or sludge, for agriculture. this p ... | 2015 | 25786255 |
| estimating the attack rate of pregnancy-associated listeriosis during a large outbreak. | in 2011, a multistate outbreak of listeriosis linked to contaminated cantaloupes raised concerns that many pregnant women might have been exposed to listeria monocytogenes. listeriosis during pregnancy can cause fetal death, premature delivery, and neonatal sepsis and meningitis. little information is available to guide healthcare providers who care for asymptomatic pregnant women with suspected l. monocytogenes exposure. | 2015 | 25784782 |
| prospecting russula senecis: a delicacy among the tribes of west bengal. | russula senecis, a worldwide distributed mushroom, is exclusively popular among the tribal communities of west bengal for food purposes. the present study focuses on its reliable taxonomic identification through macro- and micro-morphological features, dna barcoding, confirmation of its systematic placement by phylogenetic analyses, myco-chemicals and functional activities. for the first time, the complete internal transcribed spacer region of r. senecis has been sequenced and its taxonomic posi ... | 2015 | 25780764 |
| a new bovine conjunctiva model shows that listeria monocytogenes invasion is associated with lysozyme resistance. | listerial keratoconjunctivitis ('silage eye') is a wide spread problem in ruminants causing economic losses to farmers and impacts negatively on animal welfare. it results from direct entry of listeria monocytogenes into the eye, often following consumption of contaminated silage. an isolation protocol for bovine conjunctival swabbing was developed and used to sample both infected and healthy eyes bovine eyes (n=46). l. monocytogenes was only isolated from one healthy eye sample, and suggests th ... | 2015 | 25778543 |
| stability of sublethal acid stress adaptation and induced cross protection against lauric arginate in listeria monocytogenes. | the stability of acid stress adaptation in listeria monocytogenes and its induced cross protection effect against gras (generally recognized as safe) antimicrobial compounds has never been investigated before. in the present study, the acid stress adaptation in l. monocytogenes was initially induced in ph 5.0 tryptic soy broth supplemented with 0.6% yeast extract (tsb-ye) at 37 °c. subsequently, the stability of acid stress adaptation, which was defined as the capacity to maintain its acquired a ... | 2015 | 25777680 |
| avoidance of autophagy mediated by plca or acta is required for listeria monocytogenes growth in macrophages. | listeria monocytogenes is a facultative intracellular pathogen that escapes from phagosomes and grows in the cytosol of infected host cells. most of the determinants that govern its intracellular life cycle are controlled by the transcription factor prfa, including the pore-forming cytolysin listeriolysin o (llo), two phospholipases c (plca and plcb), and acta. we constructed a strain that lacked prfa but expressed llo from a prfa-independent promoter, thereby allowing the bacteria to gain acces ... | 2015 | 25776746 |
| metabolic gene expression shift by listeria monocytogenes in coculture biofilms. | coculture communities of microbes are more realistic and common in nature than in laboratory-grown pure cultures. in a mixed community, when resources with a potential role in growth are shared, conflict (as a consequence of competition) or cooperation is certain. in our study, this situation of conflict and cooperation was explored to understand the population dynamics and community behavior of listeria monocytogenes. the social behavioral response of l. monocytogenes to the presence of bacillu ... | 2015 | 25776109 |
| irf8 directs stress-induced autophagy in macrophages and promotes clearance of listeria monocytogenes. | autophagy, activated by many stresses, plays a critical role in innate immune responses. here we show that interferon regulatory factor 8 (irf8) is required for the expression of autophagy-related genes in dendritic cells. furthermore in macrophages, irf8 is induced by multiple autophagy-inducing stresses, including ifnγ and toll-like receptor stimulation, bacterial infection, starvation and by macrophage colony-stimulating factor. irf8 directly activates many genes involved in various steps of ... | 2015 | 25775030 |
| erratum to: lmo0171, a novel internalin-like protein, determines cell morphology of listeria monocytogenes and its ability to invade human cell lines. | 2015 | 25773205 | |
| tea and soybean extracts in combination with milk fermentation inhibit growth and enterocyte adherence of selected foodborne pathogens. | this study examined the antibacterial and anti-adhesive properties of pure plant extracts (ppes) of green tea (gt), black tea (bt) and soybean individually or in combination with milk. fermented phenolic enriched-milk (fpem) was prepared by combining ppes with milk and fermented with lactic acid bacteria. antimicrobial activity of extracts was evaluated by broth-dilution and agar diffusion assay. anti-adhesive property of extracts was evaluated in caco-2 cell model. results from antibacterial te ... | 2015 | 25766833 |
| lactic acid bacteria: promising supplements for enhancing the biological activities of kombucha. | kombucha is sweetened black tea that is fermented by a symbiosis of bacteria and yeast embedded within a cellulose membrane. it is considered a health drink in many countries because it is a rich source of vitamins and may have other health benefits. it has previously been reported that adding lactic acid bacteria (lactobacillus) strains to kombucha can enhance its biological functions, but in that study only lactic acid bacteria isolated from kefir grains were tested. there are many other natur ... | 2015 | 25763303 |
| [the metabolism of the innate immunity cells in bacterial infections]. | metabolic activity of innate immunity cells infected by various doses of gram-negative (yersinia pseudotuberculosis, salmonella enteritidis) and gram-positive (staphylococcus aureus, listeria monocytogenes) bacteria has been investigated. using various animal models we found that during the initial period (up to 2 days) changes of infection in cellular responses depend on the type of the pathogen. in response to infection caused by gram-negative bacteria predominant neutrophil accumulation in th ... | 2015 | 25762604 |
| a novel therapy for melanoma developed in mice: transformation of melanoma into dendritic cells with listeria monocytogenes. | listeria monocytogenes is a gram-positive bacteria and human pathogen widely used in cancer immunotherapy because of its capacity to induce a specific cytotoxic t cell response in tumours. this bacterial pathogen strongly induces innate and specific immunity with the potential to overcome tumour induced tolerance and weak immunogenicity. here, we propose a listeria based vaccination for melanoma based in its tropism for these tumour cells and its ability to transform in vitro and in vivo melanom ... | 2015 | 25760947 |
| gas chromatography-mass spectrometry characterisation of the anti-listeria components of garcinia kola seeds. | adsorption chromatography was used to separate the bioactive constituents of the crude n-hexane extract of garcinia kola seeds. the silica gel 60 column fractions were eluted using the solvent combination of benzene: ethanol : ammonium hydroxide (bea) in the ratio combination of 36 : 4 : 0.4 v/v. the fractions were tested for anti-listeria activities by determining their mic50, mic90 or mic against 4 listeria isolates. the fractions were labelled bea1 to bea5 and 3 out of the 5 fractions eluted ... | 2015 | 25757343 |
| antibiotic resistance of listeria monocytogenes isolated from meat-processing environments, beef products, and clinical cases in brazil. | the present study aimed to assess the antimicrobial resistance and the presence of virulence markers in 137 listeria monocytogenes isolates obtained from meat-processing environments, beef products, and clinical cases. all isolates were subject to molecular serogrouping and their antibiotic resistance profiles were assessed against 12 antimicrobials. in addition, isolates were subjected to detection of virulence marker genes (inla, inlc, inlj). the isolates were classified into serogroups 4b, 4d ... | 2015 | 25756759 |
| antimicrobial action of oleanolic acid on listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. | this study investigated the antimicrobial action of oleanolic acid against listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. to determine the cytotoxicity of oleanolic acid, hep-2 cells were incubated with oleanolic acid at 37°c. mics (minimal inhibition concentrations) for l. monocytogenes, e. faecium, and e. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid a ... | 2015 | 25756202 |
| potential probiotic attributes of a new strain of bacillus coagulans cgmcc 9951 isolated from healthy piglet feces. | a new strain of bacillus coagulans cgmcc 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including escherichia coli o8, staphylococcus aureus, salmonella enterica subsp. enterica serovar enteritidis, streptococcus suis, listeria monocytogenes and pasteurella multocida, was isolated from healthy piglet feces. in adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of b. subtilis jt143 and l. acidophilus ly24 res ... | 2015 | 25752235 |
| cxcr3 blockade protects against listeria monocytogenes infection-induced fetal wastage. | mammalian pregnancy requires protection against immunological rejection of the developing fetus bearing discordant paternal antigens. immune evasion in this developmental context entails silenced expression of chemoattractant proteins (chemokines), thereby preventing harmful immune cells from penetrating the maternal-fetal interface. here, we demonstrate that fetal wastage triggered by prenatal listeria monocytogenes infection is driven by placental recruitment of cxcl9-producing inflammatory ne ... | 2015 | 25751061 |
| novel inhibitor discovery and the conformational analysis of inhibitors of listeriolysin o via protein-ligand modeling. | increasing bacterial resistance to available antibiotics makes the discovery of novel efficacious antibacterial agents a priority. a previous report showed that listeriolysin o (llo) is a critical virulence factor and suggested that it is a target for developing anti-virulence drugs against listeria monocytogenes infections. in this study, we report the discovery of llo natural compound inhibitors with differential activity by using hemolysis assay. the mechanism of action of the inhibitors was ... | 2015 | 25749652 |
| commercially available rapid methods for detection of selected food-borne pathogens. | generally, the enumeration and isolation of food-borne pathogens is performed using culture-dependent methods. these methods are sensitive, inexpensive, and provide both qualitative and quantitative assessment of the microorganisms present in a sample, but these are time-consuming. for this reason, researchers are developing new techniques that allow detection of food pathogens in shorter period of time. this review identifies commercially available methods for rapid detection and quantification ... | 2016 | 25749054 |
| rapid identification and source-tracking of listeria monocytogenes using maldi-tof mass spectrometry. | listeria monocytogenes is an important foodborne pathogen responsible for the sometimes fatal disease listeriosis. public health concerns and stringent regulations associated with the presence of this pathogen in food and food processing environments underline the need for rapid and reliable detection and subtyping techniques. in the current study, the application of matrix assisted laser desorption/ionisation-time-of-flight mass spectrometry (maldi-tof ms) as a single identification and source- ... | 2015 | 25747262 |
| label-free impedimetric detection of listeria monocytogenes based on poly-5-carboxy indole modified ssdna probe. | listeria monocytogenes is a life threatening pathogenic bacteria concerned with human health. the accurate and rapid detection of l. monocytogenes is required for preventing of listeriosis. in this study, dna sensing probe based on conducting polymer poly-5-carboxy indole (5c pin) was developed for the detection of l. monocytogenes hlya gene responsible for pathogenicity. the probe sequences (24 mer ssdna) were covalently immobilized on 5c pin via n-(3-dimethylaminopropyl)-n'-ethylcarbodiimidehy ... | 2015 | 25746904 |
| microbiological contamination of dried and lyophilized garlic as a potential source of food spoilage. | garlic is valued more for its flavoring and used in a wide variety of foods. in food technology, fresh garlic is not used, but instead its processed forms, most often dried and lyophilized, are utilized. the quality and safety of the final product largely depends on their microbiological quality. this research has provided information about effect of garlic fixation methods and provided information about effect of microbiological contamination of garlic used as a spice for quality of garlic mayo ... | 2015 | 25745260 |
| microbiological and shelf life characteristics of eviscerated and vacuum packed freshwater catfish (ompok pabda) during chill storage. | the effect of vacuum packaging technique on the shelflife of eviscerated pabda catfish (ompok pabda) during chill storage at 4 ± 2 °c was studied. the shelflife of pabda fish was predicted based on the cumulative observations of biochemical, microbiological and sensory parameters. there was no significant difference in the lipid quality parameters studied between the samples during the chill storage period, whereas, vacuum packaging significantly improved the sensory and microbiological quality. ... | 2015 | 25745210 |
| genome sequences of five disinfectant-resistant listeria monocytogenes strains from two iberian pork-processing plants. | we announce the draft genome sequences of five listeria monocytogenes strains from two iberian pork-processing plants located in spain that were distinguished by their resistance to benzalkonium chloride. these strains seem highly adapted to the meat-processing environment according to their persistence and transmission capabilities. | 2015 | 25745005 |
| whole-genome sequence of listeria monocytogenes serovar 4b strain izsam_lm_hs2008, isolated from a human infection in italy. | listeria monocytogenes is one of the most important foodborne pathogens. in this report, we present the complete and annotated genome of l. monocytogenes sequence type 06 (st06) serovar 4b strain izsam_lm_hs2008, isolated from an adult immunocompetent patient who developed the disease and died. | 2015 | 25744995 |
| detection of foodborne bacterial pathogens from individual filth flies. | there is unanimous consensus that insects are important vectors of foodborne pathogens. however, linking insects as vectors of the pathogen causing a particular foodborne illness outbreak has been challenging. this is because insects are not being aseptically collected as part of an environmental sampling program during foodborne outbreak investigations and because there is not a standardized method to detect foodborne bacteria from individual insects. to take a step towards solving this problem ... | 2015 | 25742168 |
| listeria monocytogenes meningitis in an immunocompromised patient. | this report describes a case of meningitis caused by listeria monocytogenes in a stem cell transplant recipient on immunosuppressive therapy for cutaneous chronic graft-versus host disease. a 59-year-old woman had undergone allogeneic stem cell transplantation (from a matched unrelated donor) 13 months previously for chronic lymphocytic leukemia. she was on regular hematologic follow-up. though her previous malignancy has been in remission, she was immunosuppressed due to the pharmacological tre ... | 2015 | 25742155 |
| effect of chitosan on spoilage bacteria, escherichia coli and listeria monocytogenes in cured chicken meat. | the effects of essential oil (eo) of oregano and chitosan on the microbial quality and growth inhibition of listeria monocytogenes and escherichia coli o157:h7 in the iranian traditional ready-to-barbecue chicken was evaluated. thus, three groups of samples were prepared. one of them was considered to evaluate for aerobic plate count (apc), lactic acid bacteria (lab), psychrophilic and enterobacteriacae counts and the other two groups were inoculated with e. coli o157:h7 and l. monocytogenes 4b ... | 2015 | 25735728 |
| broad spectrum anti-microbial compounds producing bacteria from coast of qingdao bays. | anti-microbial resistance burden and hazard associated with chemical treatment of infections demanded for new anti-microbial natural products. marine associated microorganisms are the enormous source of bioactive compounds. in this study we have isolated 272 marine bacteria among them 136 (50%) were antagonistic to at least one of the four pathogenic strains listeria monocytogenes, vibrio cholerae, e. coli and s. aureus. only two strains exhibited antibacterial activity against all four test str ... | 2015 | 25730803 |
| molecular cloning and characterization of autophagy-related gene tmatg8 in listeria-invaded hemocytes of tenebrio molitor. | macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. currently 36 autophagy-related genes (atg) and their homologs have been characterized in yeast and higher eukaryotes, including insects. in the present study, we identified and functionally characterized the immune function of an atg8 homolog in a coleopteran insect, tenebri ... | 2015 | 25727880 |
| outbreak investigation identifies a single listeria monocytogenes strain in sheep with different clinical manifestations, soil and water. | listeria (l.) monocytogenes causes orally acquired infections and is of major importance in ruminants. little is known about l. monocytogenes transmission between farm environment and ruminants. in order to determine potential sources of infection, we investigated the distribution of l. monocytogenes genetic subtypes in a sheep farm during a listeriosis outbreak by applying four subtyping methods (maldi-tof-ms, mlst, mlva and pfge). l. monocytogenes was isolated from a lamb with septicemia and f ... | 2015 | 25726302 |
| comparative proteomic analysis of listeria monocytogenes atcc 7644 exposed to a sublethal concentration of nisin. | listeria monocytogenes infections have been frequently reported in many food poisoning outbreaks around the world. in this work, the protein repertoires of l. monocytogenes atcc 7644 cells treated or not with a 10(-3)mg/ml nisin sublethal concentration, established by antimicrobial susceptibility tests, were analyzed by lc-ms/ms. overall, 179 proteins were identified, 9 of them more abundant in nisin-treated samples, and 4 more abundant in non-treated control samples. in nisin treated cells, pro ... | 2015 | 25724729 |
| previous physicochemical stress exposures influence subsequent resistance of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes to ultraviolet-c in coconut liquid endosperm beverage. | this study investigated the influences of prior exposures to common physicochemical stresses encountered by microorganisms in food and food processing ecologies such as acidity, desiccation, and their combinations, on their subsequent susceptibility towards uv-c treatment in coconut liquid endosperm beverage. cocktails of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes were separately subjected to gradually acidifying environment (final ph 4.46), exposed to abrupt desic ... | 2015 | 25723813 |
| plasmid-borne cadmium resistant determinants are associated with the susceptibility of listeria monocytogenes to bacteriophage. | listeria monocytogenes is an intracellular pathogen causing gastroenteritis, central nervous system infections and abortions. chromosomal virulence determinants have been extensively investigated. however, the function of genes encoded by plasmids in l. monocytogenes has not been fully understood. in this study, we determined the prevalence and molecular profile of plasmids in food isolates of l. monocytogenes and examined the contribution of four plasmid-borne cadmium-resistant genes to the sus ... | 2015 | 25721472 |
| whole-genome sequences of 12 clinical strains of listeria monocytogenes. | listeria monocytogenes is a foodborne pathogen of global concern due to the high mortality rate among immunocompromised patients. whole-genome sequences of 12 strains of l. monocytogenes from humans were reported. the availability of these genomes should provide useful information on the evolutionary history and genetic diversity of l. monocytogenes. | 2015 | 25720683 |
| ceramide and sphingosine in pulmonary infections. | acid sphingomyelinase and ceramide have previously been shown to play a central role in infections with neisseria gonorrhoeae, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, escherichia coli, and mycobacterium avium. recent studies have extended the role of sphingolipids in bacterial infections and have demonstrated that ceramide and sphingosine are central to the defense of lungs against bacterial pathogens. ceramide accumulates in the airway epit ... | 2015 | 25720061 |
| prevalence of bacteria and absence of anisakid parasites in raw and prepared fish and seafood dishes in spanish restaurants. | this study evaluated the presence of bacteria and anisakid parasites in 45 samples of raw anchovies in vinegar, a dish widely eaten in spain, and in 227 samples of cooked fish and cephalopods served in spanish food service establishments. our analysis showed that, according to european and spanish regulation, 14 to 30% of the prepared fish and cephalopod dishes exceeded the maximum allowable level for mesophilic aerobic counts, and 10 to 40% of these samples exceeded the allowable levels for ent ... | 2015 | 25719890 |
| differentiation of different mixed listeria strains and also acid-injured, heat-injured, and repaired cells of listeria monocytogenes using fourier transform infrared spectroscopy. | fourier transform infrared (ft-ir) spectroscopy was used to differentiate mixed strains of listeria monocytogenes and mixed strains of l. monocytogenes and listeria innocua. ft-ir spectroscopy was also applied to investigate the hypothesis that heat-injured and acid-injured cells would return to their original physiological integrity following repair. thin smears of cells on infrared slides were prepared from cultures for mixed strains of l. monocytogenes, mixed strains of l. monocytogenes and l ... | 2015 | 25719878 |
| seek and destroy process: listeria monocytogenes process controls in the ready-to-eat meat and poultry industry. | the majority of human listeriosis cases appear to be caused by consumption of ready-to-eat (rte) foods contaminated at the time of consumption with high levels of listeria monocytogenes. although strategies to prevent growth of l. monocytogenes in rte products are critical for reducing the incidence of human listeriosis, control of postprocessing environmental contamination of rte meat and poultry products is an essential component of a comprehensive l. monocytogenes intervention and control pro ... | 2015 | 25710164 |
| first two domains at the lp_1643 protein n terminus inhibit pathogen adhesion to porcine mucus in vitro. | gastrointestinal probiotics are important members of intestinal microflora in both healthy animals and human beings, and these bacteria may reduce the risk of infection caused by certain opportunistic pathogens through exclusive inhibition, competition, and displacement. the lp_1643 protein on the cell surface of lactobacillus plantarum wcfsi was assumed to possess a mucus-binding capability. this study aimed to determine if purified his-n2 protein exclusively inhibits pathogen adhesion to porci ... | 2015 | 25710153 |
| assessing the survival of listeria monocytogenes in a domestic freezer by analyzing subsequent growth at 30°c using a novel reference method. | listeria monocytogenes is a serious pathogen capable of extensive survival under frozen storage. using optical density and multiple initial inocula in multiple identically prepared microtiter plates, the effect of storage time at -22°c on the subsequent growth at 30°c of the organism when defrosted was studied using a technique that compared the growth (through time to detection) of a test plate (previously frozen) with that of an identically prepared control plate, analyzed at the start of the ... | 2015 | 25710150 |
| survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes on raw peanut and pecan kernels stored at -24, 4, and 22°c. | cocktails of lawn-collected cells were used to determine the survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes on the surface of raw peanut and pecan kernels. kernels were inoculated with mixtures of four to five strains at 3 or 6 log cfu/g, dried at room temperature, and then stored at -24 ± 1, 4 ± 2, and 22 ± 1°c for 28 or 365 days. in most cases, rates of decline of the pathogens did not differ significantly between the two inoculum concentrations in the 28-day stud ... | 2015 | 25710147 |
| estimation of listeria monocytogenes and escherichia coli o157:h7 prevalence and levels in naturally contaminated rocket and cucumber samples by deterministic and stochastic approaches. | the aims of the present study were to determine the prevalence and levels of listeria monocytogenes and escherichia coli o157:h7 in rocket and cucumber samples by deterministic (estimation of a single value) and stochastic (estimation of a range of values) approaches. in parallel, the chromogenic media commonly used for the recovery of these microorganisms were evaluated and compared, and the efficiency of an enzyme-linked immunosorbent assay (elisa)-based protocol was validated. l. monocytogene ... | 2015 | 25710146 |