Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| purification of cytochrome p-450 from n-hexadecane-grown acinetobacter calcoaceticus. | a convenient procedure for the purification of cytochrome p-450 from an n-alkane-assimilating strain of the bacterial species acinetobacter calcoaceticus has been elaborated. the cytochrome p-450 of n-hexadecane-grown cells was found to be distributed in cell-free extracts among particulate and soluble subcellular fractions. for isolation, cytochrome p-450 was extracted from particulate fractions by addition of triton x-100 to the buffer. subsequently, purification to an apparently homogeneous s ... | 1989 | 2546537 |
| cloning and expression in escherichia coli of an esterase-coding gene from the oil-degrading bacterium acinetobacter calcoaceticus rag-1. | a putative esterase gene (est) from acinetobacter calcoaceticus rag-1 has been cloned into escherichia coli. esterase-positive clones exhibited high levels of esterase activity even in intact cells. in addition, expression of the est gene conferred on e. coli the ability to grow on simple triglycerides such as triacetin (tac). the original esterase-positive plasmid pra17 carried a 2.2-kb insert from a partial mboi digest of rag-1 dna, which gave a single band with rag-1 dna following southern hy ... | 1989 | 2545531 |
| selection of glucose-assimilating variants of acinetobacter calcoaceticus lmd 79.41 in chemostat culture. | glucose metabolism has been studied in two strains of acinetobacter calcoaceticus. strain lmd 82.3, was able to grow on glucose and possessed glucose dehydrogenase (ec 1.1.99.17). glucose oxidation by whole cells was stimulated by pqq, the prosthetic group of glucose dehydrogenase. pqq not only increased the rate of glucose oxidation and gluconic acid production but also shortened the lag phase for growth on glucose. strain lmd 79.41 also possessed glucose dehydrogenase but was unable to grow on ... | 1989 | 2545167 |
| purification and antimicrobial properties of three defensins from rat neutrophils. | three cysteine-rich cationic peptides, designated ratnp-1, ratnp-3, and ratnp-4, were purified from an acid extract of rat polymorphonuclear neutrophils, sequenced, and tested for antimicrobial activity. the peptides ranged from 29 to 32 amino acids in length (mr, 3,252 to 3,825), and each contained all eight invariantly conserved "framework" residues that are characteristic of defensins. each of the peptides killed escherichia coli ml-35, acinetobacter calcoaceticus hon-1, staphylococcus aureus ... | 1989 | 2543629 |
| aminoglycoside-o-phosphotransferase (aph (3'')) activity in a clinical isolate of acinetobacter calcoaceticus. | an isolate of acinetobacter calcoaceticus var. anitratus containing an aminoglycoside-aminocyclitol antibiotic-modifying enzyme, which phosphorylates streptomycin at the 3" position, is described. the enzyme, aph(3"), was identified on the basis of its substrate specificity; in particular, its ability to phosphorylate streptomycin but not streptidine. | 1989 | 2538930 |
| acinetobacter calcoaceticus genes involved in biosynthesis of the coenzyme pyrrolo-quinoline-quinone: nucleotide sequence and expression in escherichia coli k-12. | synthesis of the coenzyme pyrrolo-quinoline-quinone (pqq) from acinetobacter calcoaceticus requires the products of at least four different genes. in this paper we present the nucleotide sequence of a 5,085-base-pair dna fragment containing these four genes. within the dna fragment three reading frames are present, coding for proteins of mr 10,800, 29,700, and 43,600 and corresponding to three of the pqq genes. in the dna region where the fourth pqq gene was mapped the largest possible reading f ... | 1989 | 2536663 |
| [nosocomial infections caused by acinetobacter. epidemiology and therapeutic difficulties]. | nosocomial infections due to acinetobacter calcoaceticus are not easy to treat particularly in intensive-care and surgical units. our study included 33 cases of nosocomial infections which developed during 1987 in the surgical intensive care unit and in the urology department. acinetobacter was isolated from various types of nosocomial infections: urinary tract infections (43 per cent); septicaemia (15 per cent); surgical infections (27 per cent) and respiratory tract infections (15 per cent). f ... | 1989 | 2521935 |
| [hydrophilic-hydrophobic properties of microorganisms under various culturing conditions]. | the index of hydrophobicity and the hydrophilic-hydrophobic properties of seven microbial cultures were determined by studying their adhesion to n-hexadecane. the index of hydrophobicity was shown to be a stable characteristic of a culture grown in media with different carbon sources. as was found for escherichia coli k-12 and acinetobacter calcoaceticus k-9 whose hydrophobicity indicates were quite different, the character of cell hydration was virtually independent of the growth phase and did ... | 1989 | 2511395 |
| in vitro and in vivo antibacterial activities of bo-1341, a new antipseudomonal cephalosporin. | bo-1341, a new antipseudomonal semisynthetic cephalosporin, was evaluated for in vitro and in vivo antibacterial activities in comparison with ceftazidime, cefotaxime, and cefoperazone. the in vitro activity of bo-1341 was generally superior or comparable to the activities of the reference antibiotics against clinical isolates of the family enterobacteriaceae. bo-1341 was highly active against pseudomonas aeruginosa (mic for 90% of the strains tested, 1.56 micrograms/ml), pseudomonas maltophilia ... | 1989 | 2510590 |
| in vitro activity of fleroxacin and 6 other antimicrobials against acinetobacter anitratus. | the in vitro activity of the 4-quinolone compound fleroxacin (ro-23-6240) was compared with that of enoxacin, ofloxacin, cefepime (bmy-28142), ceftazidime, ceftriaxone, and tobramycin against a total of 30 recent clinical isolates of acinetobacter calcoaceticus subsp. anitratum. susceptibility testing (mic50/mic90) was performed by a microtiter broth dilution method and the combination effect of ceftriaxone plus tobramycin was studied by checkerboard titration in microtiter trays. fleroxacin inh ... | 1989 | 2507235 |
| [antibiotic resistance of gram-negative bacteria isolated at a hospital intensive care unit]. | the antibiotic sensitivity of gram-negative facultatively pathogenic bacteria isolated between 1982-1986 in the respiratory intensive care unit of the 3rd department of pediatry of the "lászló" hospital (budapest) has been studied. pseudomonas aeruginosa, klebsiella, acinetobacter calcoaceticus and acinetobacter lwoffii were most frequently isolated both from the clinical samples (total 56.9%) and in samples obtained from hospital-hygienic check-examinations. 70-100% of the individual species wa ... | 1989 | 2498804 |
| [evaluation of the treatment with oral ciprofloxacin: experience with 24 patients]. | we have evaluated the clinical effectiveness and possible side effects of oral ciprofloxacin therapy in 24 patients (11 females and 13 males). two episodes from overall 25 infections were excluded of clinical and microbiological evaluation but were assessed regarding possible side effects. in most patients, the clinical condition was stable at the beginning of therapy. only 3 patients had nonfatal diseases. seventeen episodes were urinary tract infections (four of them with concomitant bacteremi ... | 1989 | 2490722 |
| [evaluation of sen-sir, a new microsystem for the identification and determination of bacterial sensitivity]. | the use of semiautomatic methods for identification and study of bacterial sensitivity to antimicrobials is increasingly widespread in clinical microbiology. in the present study, the evaluation of a new micromethod (sen-sir, bbl) for the identification and determination of bacterial sensitivity is reported. 115 clinical isolates of enterobacteria, 10 strains of acinetobacter calcoaceticus and 57 gram positive cocci, previously identified as belonging to the geni staphylococcus and streptococcus ... | 1989 | 2490681 |
| [control of infection in the odontostomatologic field. possibility of decontamination of critical surfaces]. | after a brief description of the sources and procedures of transmission of infections in the odontostomatological field, the authors illustrate the degree of contamination of a range of surfaces presented by odontological instruments. this is followed by a description of the possibility of a disinfecting treatment using two products one based on iodoform and the other on quaternary ammonium. prior to this disinfection treatment, the surfaces examined presented a level of microbial contamination ... | 1989 | 2483897 |
| [evaluation of the effectiveness of disinfectants used in several neapolitan hospitals]. | among the various and complex aspects concerning the etiology of nosocomial infections, a relevant role is played by the indiscriminate and incorrect use of disinfectants: they may change paradoxically from devices used for nosocomial infection prevention into carriers of infections. critical situations are: a) inadequate cleaning of the container; b) inaccurate concentrations set up; c) use of unsterilized water as a diluent; d) introduction of gauze or cotton flocks into the container; e) disi ... | 1989 | 2483631 |
| [study of clinical strains of microorganisms in a scanning electron microscope]. | the authors present an available method for preparing bacterial cells for examination in a scanning electron microscope. the method has been tried with clinical strains of s. aureus, p. aeruginosa, acinetobacter calcoaceticus v. anitratus. morphologic features of various types of microorganisms are described. | 1989 | 2470972 |
| antimicrobial mechanisms against acinetobacter calcoaceticus of rat polymorphonuclear leukocyte granule extract. | the antimicrobial mechanisms of rat polymorphonuclear leukocyte granule extract and isolated extract fractions against acinetobacter calcoaceticus were examined. crude granule extract and a fraction containing low-molecular-weight cationic peptides (peak d) reduced the viability of a. calcoaceticus and inhibited the uptake of radiolabeled macromolecule precursors by cells. the inhibitory activity observed with peak d was not as great as that of crude granule extract containing equivalent amounts ... | 1988 | 2449397 |
| nucleotide sequence, secondary structure and evolution of the 5s ribosomal rna from five bacterial species. | the nucleotide sequences of the 5s ribosomal rnas of the bacteria agrobacterium tumefaciens, alcaligenes faecalis, pseudomonas cepacia, aquaspirillum serpens and acinetobacter calcoaceticus have been determined. the sequences fit in a generally accepted model for 5s rna secondary structure. however, a closer comparative examination of these and other bacterial 5s rna primary structures reveals the potential of additional base pairing and of multiple equilibria between a set of slightly different ... | 1985 | 2408888 |
| investigation of an outbreak of acinetobacter calcoaceticus var. anitratus infections in an adult intensive care unit. | 1990 | 2403220 | |
| oral pefloxacin mesylate in the treatment of continuous ambulatory peritoneal dialysis associated peritonitis: an open non-comparative study. | twenty-two (15 female and 7 male) patients with continuous ambulatory peritoneal dialysis (capd) associated peritonitis were entered into an open non-comparative evaluation of oral pefloxacin mesylate. an initial loading dose of 800 mg was followed by 400 mg twice daily. five patients were subsequently excluded or withdrawn. the mean age of the patients was 44 +/- 12.6 years. all three gram-negative infections (escherichia coli, acinetobacter calcoaceticus, serratia liquefaciens) and all three c ... | 1990 | 2373667 |
| in vitro studies of ciprofloxacin and survey of resistance patterns in current isolates. | we studied the activity of ciprofloxacin and other antibiotics against both routine and multiresistant (multi-r) clinical isolates. ciprofloxacin inhibited more than 98% of most species of enterobacteriaceae at a concentration of 2 micrograms/ml. only acinetobacter calcoaceticus, and to a much lesser degree, providencia and serratia, were resistant. most pseudomonas aeruginosa isolates were susceptible. only 1% of staphylococci were resistant; the test panel included 1200 mrsa. for most species ... | 1990 | 2369816 |
| airborne microbial contents in two types of swine confinement buildings in quebec. | airborne microorganisms were isolated with a sampler in two types of swine confinement buildings (farrowing units and fattening units). respirable (particles less than 5 microns) and total dust fractions were obtained. samplings were repeated every 2 weeks for a total of 6 samplings per unit between january and april. the predominant microorganisms isolated were bacteria (up to 1.25 x 10(6) cfu/m3) with an important fraction in the respirable size range (up to 0.5 x 10(6) cfu/m3). only small qua ... | 1990 | 2353639 |
| concerning the sensitivity of acinetobacter calcoaceticus. | the strains of acinetobacter calcoaceticus, var. anitratus (a. c. a.) were isolated in the nosocomial environment as an opportune pathogen. the therapy of choice may be determined after in vitro tests. our results show following therapeutical possibilities: beta-lactam antibiotics--cephalosporins of iiird generation (cefotaxime), also combinations of antimicrobials have shown good results: amoxycillin or ticarcillin with clavulanic acid. best synergistic effect was found in combination ticarcill ... | 1990 | 2351822 |
| [evaluation of imipenem/cilastatin sodium in the treatment of respiratory tract infections]. | imipenem/cilastatin sodium (ipm/cs) was administered to 55 patients with respiratory tract infections (rti). a clinical evaluation of ipm/cs was carried out in 51 patients, 28 with pneumonia, 4 with pulmonary abscess, 1 with pyothorax, 6 with bronchitis, 9 with bronchiectasis, 1 with diffuse panbronchiolitis and 2 with rti with chronic obstructive pulmonary disease, and the clinical efficacy rate was 78.4%. causative organisms were isolated in 23 strains out of 20 patients, such as staphylococcu ... | 1990 | 2348550 |
| identification and nucleotide sequence of the acinetobacter calcoaceticus encoded trpe gene. | the trpe gene from acinetobacter calcoaceticus encoding the anthranilate synthase component i was cloned, identified by deletion analysis and sequenced. it encodes a predicted polypeptide of 497 amino acids with a calculated molecular weight of 55,323. its primary structure shows 49% identical amino acids with the enzyme from clostridium thermocellum, 45% with that of thermus thermophilus and only 35% with that of escherichia coli. the codon usage of the trpe genes encoding the most homologous e ... | 1990 | 2338941 |
| [studies on respiratory infections in primary care clinic (ii). distribution and antibiotic sensitivity to 45 agents of bacteria isolated from patients with respiratory infections visiting a doctor in private practice]. | the bacteriology of the isolates from the sputum or the throat swab of patients with respiratory infections visiting a doctor in private practice in sendai city during the period from march in 1988 to february in 1989 was documented, and their sensitivity to 45 antimicrobial agents was determined. of the 568 patients, 514 cases had acute pharyngitis, 8 cases each had acute tonsillitis and acute bronchitis, 7 cases were acute pneumonia, 6 cases had herpangina, 18 cases had hand-foot-mouth disease ... | 1990 | 2335751 |
| the in-vitro activity of ceftibuten against 475 clinical isolates of gram-negative bacilli, compared with cefuroxime and cefadroxil. | the in-vitro activity of ceftibuten was compared with cefuroxime and cefadroxil against 475 clinically-significant, epidemiologically-distinct isolates of gram-negative bacilli: 170 from blood, 212 from urine and 93 from a supplementary collection of multiply-resistant strains known to have resistance plasmids, to have caused sporadic or epidemic nosocomial infection, or both. ceftibuten mics ranged from 0.003 to greater than 32 mg/l, with a modal mic of 0.01 mg/l: 95% of all isolates had ceftib ... | 1990 | 2329100 |
| an evolutionary comparison of acinetobacter calcoaceticus trpf with trpf genes of several organisms. | the deduced amino acid sequence of acinetobacter calcoaceticus n-(5'-phosphoribosyl) anthranilate isomerase (prai), which is coded by trpf, was compared with trpf of caulobacter crescentus, escherichia coli, bacillus subtilis, saccharomyces cerevisiae, neurospora crassa, and aspergillus nidulans. sixty percent of identical or similar amino acids were located in alpha/beta tim (triose-phosphate isomerase) barrels and in residues important in substrate binding and catalysis. in addition, the analy ... | 1990 | 2299982 |
| dna sequences of genes encoding acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of dna sequences within genes during their evolutionary divergence. | the dna sequence of a 2,391-base-pair hindiii restriction fragment of acinetobacter calcoaceticus dna containing the pcachg genes is reported. the dna sequence reveals that a. calcoaceticus pca genes, encoding enzymes required for protocatechuate metabolism, are arranged in a single transcriptional unit, pcaefdbchg, whereas homologous genes are arranged differently in pseudomonas putida. the pcag and pcah genes represent separate reading frames respectively encoding the alpha and beta subunits o ... | 1990 | 2298704 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates]. | this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ... | 1990 | 2287060 |
| the effect of physical and microbiological factors on food container leakage. | the effect of physical and microbiological factors on food container leakage was investigated in a container leakage model system (clms). the leakage of acinetobacter calcoaceticus, staphylococcus sp., pseudomonas sp., bacillus sp., a coryneform, staph. aureus, and two biotest organisms (enterobacter cloacae nc1b 8151 and ent. aerogenes mb31) was studied. the rate of bacterial leakage (log10 cells/channel/s) was greater in the presence of a partial vacuum of 51-305 mm hg than at atmospheric pres ... | 1990 | 2272940 |
| [acinetobacter as a problem pathogen in patients with long-term tocolysis]. | a hospital outbreak of acinetobacter calcoaceticus in a ward with patients with intravenous tokolysis is reported. within 2 months 9 pregnant women who had tokolysis for premature labour developed septic fever that only subsisted after the administration of tokolysis was stopped. in 7 cases blood culture was positive for acinetobacter calcoaceticus. this endemic outbreak was responsible for premature deliveries in four cases, leading top post partum death of two infants. source identification wa ... | 1990 | 2264412 |
| pefloxacin efficacy in gram-negative bacillary meningitis. | sixteen patients with acute meningitis caused by gram-negative bacteria were treated with pefloxacin intravenously. the age range of the patient group was six months to 85 years with a mean age of 40 years; three patients were children. in all but two patients meningitis was a complication of neurosurgical operations and fourteen of the sixteen had received prior therapy which was not successful. the causative organisms were: pseudomonas aeruginosa (5), acinetobacter calcoaceticus (4), klebsiell ... | 1990 | 2258348 |
| mycenon, a new metabolite from a mycena species ta 87202 (basidiomycetes) as an inhibitor of isocitrate lyase. | mycenon (c11h5cl3o3), a new inhibitor of isocitrate lyase (ec 4.1.3.1) was isolated from the culture broth of a basidiomycete, mycena sp. mycenon is a novel chlorinated benzoquinone derivative which is also active against bacteria and fungi. malate synthase (ec 4.1.3.2) the second key enzyme of the glyoxylate cycle was not affected by mycenon. isocitrate lyase preparations from plants, bacteria and fungi were sensitive. the following ki-values for mycenon have been determined: ricinus communis, ... | 1990 | 2258323 |
| [respiratory distress after endotracheal insufflation of baby powder in dogs]. | the cause of delayed respiratory distress after endotracheal insufflation of baby powder was investigated in dogs. ten dogs were divided into 2 groups. in the insufflation group (n = 6), baby powder 1g.kg-1 was insufflated through an endotracheal tube under controlled mechanical ventilation. in the control group (n = 4), artificial ventilation was continued for 36 hours without insufflation of baby powder. pao2 in the insufflation group fell until 1 hour, and then improved until 12 hours after t ... | 1990 | 2255034 |
| evidence of protein-tyrosine kinase activity in the bacterium acinetobacter calcoaceticus. | protein phosphorylation was investigated in the bacterium acinetobacter calcoaceticus both in vivo and in vitro. in cells grown with [32p]orthophosphate, several radioactive phosphoproteins were detected by gel electrophoresis and autoradiography. these proteins were shown to contain phosphoserine, phosphothreonine, and a relatively large proportion of phosphotyrosine residues. incubation of cellular extracts with [gamma-32p] atp also resulted in the phosphorylation of several proteins. at least ... | 1990 | 2250004 |
| [studies on respiratory infections in primary care clinic (iv). antibiotic sensitivity of bacteria isolated from patients with respiratory infections visiting 21 private clinics in the tohoku district of japan]. | we determined the mics of ampicillin, methicillin, cefaclor, cefixime, cefteram, ofloxacin and ciprofloxacin against a total of 1,448 strains from 11 species: 464 strains of staphylococcus aureus, 306 strains of streptococcus pneumoniae, 114 strains of streptococcus pyogenes, 37 strains of branhamella catarrhalis, 329 strains of haemophilus influenzae, 32 strains of escherichia coli, 66 strains of klebsiella pneumoniae, 26 strains of enterobacter cloacae, 20 strains of serratia marcescens, 12 st ... | 1990 | 2243194 |
| plasmid dna fingerprinting of acinetobacter calcoaceticus subspecies anitratus from intubated and mechanically ventilated patients. | forty-three intubated and mechanically ventilated patients in five intensive care units (icus) of one hospital developed respiratory colonization or infection with acinetobacter calcoaceticus subspecies anitratus over a 16-month interval. neither the frequency nor rate of a anitratus isolation exceeded the hospital endemic norms. single isolates from 34 of the patients were subtyped by plasmid dna analysis, two biotyping systems and antimicrobial susceptibility to 24 drugs. plasmid dna fingerpri ... | 1990 | 2230042 |
| cloning, mapping, and sequencing of the gene encoding escherichia coli quinoprotein glucose dehydrogenase. | escherichia coli contains pyrroloquinoline quinone-dependent glucose dehydrogenase. we cloned and sequenced the gene (gcd) encoding this enzyme and showed that the derived amino acid sequence is highly homologous to that of the gdha gene product of acinetobacter calcoaceticus. stretches of homology also exist between the amino acid sequence of e. coli glucose dehydrogenase and other pyrroloquinoline quinone-dependent dehydrogenases from several bacterial species. the position of gcd on the chrom ... | 1990 | 2228962 |
| recovery of dna from the acinetobacter calcoaceticus chromosome by gap repair. | a strain of acinetobacter calcoaceticus that demonstrates unusually high competence for natural transformation by linear dna has proven valuable for analysis of genes and gene clusters associated with aromatic catabolism. the transformation system allowed gap repair to be used to recover mutant chromosomal dna within recombinant plasmids. the sizes of the recovered fragments, 5 and 7 kilobase pairs in length, indicate that gap repair will be a useful procedure for isolation of wild-type and modi ... | 1990 | 2211536 |
| selection of acinetobacter calcoaceticus mutants deficient in the p-hydroxybenzoate hydroxylase gene (poba), a member of a supraoperonic cluster. | p-hydroxybenzoate hydroxylase, the product of the poba gene, gives rise to protocatechuate, which is metabolized by enzymes encoded by the pca operon in acinetobacter calcoaceticus. mutations in pcad prevented growth of a. calcoaceticus with succinate in the presence of p-hydroxybenzoate. mutants selected on this medium contained the original mutation in pcad and also carried spontaneous mutations in poba. these independently expressed genes were cotransformed with a frequency of 15% and thus ar ... | 1990 | 2211534 |
| molecular cloning, nucleotide sequence, and promoter structure of the acinetobacter calcoaceticus trpfb operon. | the trpfb operon from acinetobacter calcoaceticus encoding the phosphoribosyl anthranilate isomerase and the beta-subunit of tryptophan synthase has been cloned by complementation of a trpb mutation in a. calcoaceticus, identified by deletion analysis, and sequenced. it encodes potential polypeptides of 214 amino acids with a calculated molecular weight of 23,008 (trpf) and 403 amino acids with a molecular weight of 44,296 (trpb). the encoded trpb sequence shows striking homologies to those from ... | 1990 | 2211532 |
| mechanism of enhancement of microbial cell hydrophobicity by cationic polymers. | polycationic polymers have been noted for their effects in promoting cell adhesion to various surfaces, but previous studies have failed to describe a mechanism dealing with this type of adhesion. in the present study, three polycationic polymers (chitosan, poly-l-lysine, and lysozyme) were tested for their effects on microbial hydrophobicity, as determined by adhesion to hydrocarbon and polystyrene. test strains (escherichia coli, candida albicans, and a nonhydrophobic mutant, mr-481, derived f ... | 1990 | 2211502 |
| parenteral fluoroquinolones in children with life-threatening infections. | the new fluoroquinolones have not been tested in children despite their wide spectrum of in vitro activity and efficacy, because of an observed damage to cartilage in young animals. however, in some cases they may be life-saving. we present three pediatric patients with life threatening infections in whom the fluoroquinolones were used when other antibiotics failed: a seven-year-old boy with meningitis due to multiresistant acinetobacter calcoaceticus, a three-year-old boy with job's syndrome wi ... | 1990 | 2210857 |
| ciprofloxacin in the treatment of nosocomial multiply resistant acinetobacter calcoaceticus bacteremia. | twelve cases of multiply resistant acinetobacter calcoaceticus bacteremia occurred in three intensive care units in three different outbreaks. all patients were mechanically ventilated, on broad spectrum antibiotics and had central lines when bacteremia occurred. the sites of primary infection were: abdominal (n = 3); respiratory (n = 4); central lines (n = 4); cns (n = 1). in eight cases the acinetobacter strains were susceptible to ciprofloxacin only. four other strains were sensitive to amika ... | 1990 | 2210856 |
| an outbreak of acinetobacter calcoaceticus infection in a neonatal care unit. | between january and december 1988, 383 neonates were admitted to our neonatal intensive care unit. 1,991 swabs and blood cultures were tested bacteriologically. among them 90 specimens obtained from 41 patients were positive for acinetobacter calcoaceticus. during this period we discovered and treated three cases with a. calcoaceticus sepsis. three additional cases had blood cultures positive for this bacterium without demonstrating any clinical signs of infection. there is good evidence that co ... | 1990 | 2210855 |
| acinetobacter calcoaceticus from blood culture of children. | 1990 | 2210834 | |
| therapeutic effects of cefpirome, a new cephalosporin, on various models of infections in mice and rats. | cefpirome (hr 810) is a new cephalosporin with a 2,3-cyclopentenopyridine group in the 3-position side chain. it was compared with other cephem antibiotics in protective and therapeutic effects on various experimental infections, systemic and local, in mice and rats. hr 810 had more potent protective effect than ceftazidime (caz), cefoperazone (cpz), and cefotaxime (ctx) on systemic infections induced by escherichia coli ec-31, staphylococcus aureus smith, and serratia marcescens sm-6 in mice. a ... | 1990 | 2190011 |
| cross resistance to ciprofloxacin and other antimicrobial agents among clinical isolates of acinetobacter calcoaceticus biovar anitratus. | using an agar dilution assay, for 66 of 104 (63.5%) clinical isolates of acinetobacter calcoaceticus biovar anitratus, the mic of ciprofloxacin was greater than or equal to 1.0 micrograms/ml. cross resistance was demonstrable to ciprofloxacin and gentamicin (p less than 0.001), amikacin (p less than 0.01), cefotaxime (p less than 0.001), azlocillin (p less than 0.001), ceftazidime (p less than 0.001), trimethoprim-sulfamethoxazole (p less than 0.001), and minocycline (p less than 0.05). the mean ... | 1990 | 2185692 |
| analysis and nucleotide sequence of an origin of dna replication in acinetobacter calcoaceticus and its use for escherichia coli shuttle plasmids. | a shuttle plasmid for acinetobacter calcoaceticus and escherichia coli has been constructed from a cryptic a. calcoaceticus lwoffi plasmid and pbr322. it is transformed to a. calcoaceticus bd413 by natural competency, yielding about 10(6) transformants per microgram of plasmid dna. the apr and tcr genes of pbr322 are functional in a. calcoaceticus. a gene bank was constructed from chromosomal a. calcoaceticus dna and the shuttle plasmid. direct transformation to a. calcoaceticus yielded about 95 ... | 1990 | 2185139 |
| native valve endocarditis caused by acinetobacter calcoaceticus subspecies lwoffi. | a case of native valve endocarditis due to acinetobacter calcoaceticus in a patient with dental caries is presented. the aortic, mitral and tricuspid valves were affected and showed vegetation by echocardiography in the affected valves. in spite of a good response to antibiotic therapy, multiple embolisms resulted in the patient's death. an autopsy confirmed the vegetation, which was calcified and contained no bacteria. | 1990 | 2150206 |
| characterisation of a siderophore from acinetobacter calcoaceticus. | the gram-negative bacterium acinetobacter calcoaceticus was examined for production of siderophores and iron-repressible outer membrane proteins following growth in iron-restricted media. the iron chelator, 2,3-dihydroxybenzoic acid was identified in the culture supernatant by 1h nuclear magnetic resonance (nmr) spectroscopy and mass spectrometry (ms). a group of outer membrane proteins between 80 and 85 kda were induced under iron restriction. | 1990 | 2144495 |
| [comparative activity of imipenem, ceftazidime and cefotaxime against acinetobacter calcoaceticus]. | the authors compared the in vitro activity of imipenem, ceftazidime and cefotaxime against 100 strains of acinetobacter calcoaceticus isolated in 1986 and 1987. the minimal inhibitory and bactericidal concentrations (mics and mbcs) were determined by the agar dilution and broth microdilution methods respectively, with and without 50 per cent of human serum in the medium to evaluate the possible influence of protein binding. imipenem was the most active of the three drugs against acinetobacter, i ... | 1990 | 2139937 |
| [evaluation of microbial air contamination in dental areas]. | the authors carried out a survey in dental wards in order to evaluate the qualitative and quantitative microbial contamination level of the air, which is considered a crucial habitat for the transmission of many infectious diseases. a high microbial counts were found in all of the environments tested; the microorganisms isolated belonged to pathogenous species such as staphylococcus aureus, staphylococcus haemoliticus, pseudomonas aeruginosa, acinetobacter calcoaceticus var. lwoffi. it seems nec ... | 1990 | 2129101 |
| [non-hospital-acquired pneumonia caused by acinetobacter calcoaceticus]. | 1990 | 2128612 | |
| [spontaneous transformation in mixed cultures of various types of acinetobacter and during joint growth of acinetobacter calcoaceticus with escherichia coli and pseudomonas aeruginosa]. | the transfer of chromosomal and plasmid genes was studied via spontaneous transformation is mixed cultures of acinetobacter spp. it turned out that any acinetobacter strain, irrespective of its species specificity, serves as chromosomal dna donor in case the mixed culture contains competent cells of the recipient strain. no transfer took place when non-related bacteria were used as donors. we also studied the transfer into ac. calcoaceticus competent strain cells of small non-conjugative plasmid ... | 1990 | 2126520 |
| bacteriostatic and bactericidal actions of boric acid against bacteria and fungi commonly found in urine. | boric acid has been used for over 20 years to preserve urine while in transit for bacteriological examination. it has been suggested that it may be toxic for some urinary pathogens. to investigate this several strains of bacteria and fungi commonly found in urine were exposed to different concentrations of boric acid in nutrient broth. viable counts were made at the outset and at intervals for up to 24 hours at room temperature to detect bacteriostatic or bactericidal effects. at concentrations ... | 1990 | 2116453 |
| the antimicrobial activity of fleroxacin ro 23-6240, a third generation fluoroquinolone derivative, tested in vitro. | the broad antimicrobial spectrum of fleroxacin ro 23-6240 was tested on a panel of bacterial and several mycobacterial species. staph. aureus strains, gramnegative bacteria e. coli, kl. pneumoniae, salmonella spp. were found to be rather susceptible to the tested preparation in vitro. the same was true of those species which are known to feature strong natural resistance, e.g. ps. aeruginosa and acinetobacter calcoaceticus. as regards mycobacteria, complete susceptibility was recorded for 10 m. ... | 1990 | 2112571 |
| [clinical evaluation of therapy for aspiration pneumonia with imipenem/cilastatin sodium]. | in an open, prospective, multicenter trial the clinical efficacy of imipenem/cilastatin sodium (ipm/cs) for the treatment of 14 cases with aspiration pneumonia was investigated. the mean age was 75.4 years old. diseases of central nervous system were present in 11 cases, cardiovascular diseases, pulmonary diseases and diabetes mellitus in 2 cases each respectively. seven cases were community-acquired and another seven were hospital-acquired. six cases were moderate and 8 cases were severe. causa ... | 1990 | 2112207 |
| [peritonitis in continuous ambulatory peritoneal dialysis. microbiological and clinical evaluation]. | in 1976, popovich et al. described a technique of peritoneal dialysis using bottled dialysate. later oreopoulos et al. modified the technique by using plastic bags. but peritonitis still is a major and potentially serious complication of peritoneal dialysis. we have evaluated a) microbiologic diagnostic methods for infectious peritonitis, b) incidence of etiologic agents, and c) the evolution during antimicrobial treatment. eighteen patients with chronic renal failure of diverse causes were foll ... | 1990 | 2101843 |
| removal of lead from aqueous solution by acinetobacter calcoaceticus. | a bacterial strain tolerant to the presence of 400 ppm lead was isolated from digested sewage sludge. the organism was identified as acinetobacter calcoaceticus var: anitraus (98% confidence). both viable and formalin-inactivated bacterial cells could remove pb from an aqueous solution. the pb-binding ability of inactivated cells was compared with that of a commercial ion-exchange resin. amberlite ir-120. the metal-binding ability of a. calcoaceticus followed the sequence pb greater than or equa ... | 1990 | 2099791 |
| a 28 kda protein of normal mouse serum binds lipopolysaccharides of gram-negative and lipoteichoic acids of gram-positive bacteria. | a 28 kda protein from normal mouse serum known to bind to the inner core region of bacterial lipopolysaccharide (lps) was found to bind also to bacterial poly(glycerophosphate) lipoteichoic acid (lta). twenty-nine preparations of lta were isolated from 19 different bacterial species, purified, chemically analysed, and tested for their ability to bind the 28 kda protein in a complement-dependent hemolysis and hemolysis inhibition assay. all but one were active in one or both systems and one half ... | 1990 | 2099385 |
| [clinical importance of bacteremias caused by acinetobacter calcoaceticus]. | clinical data from patients with acinetobacter calcoaceticus bacteremia observed during a period of 10 years were retrospectively reviewed. these bacteremias represent the 0.66% of the total number of positive blood cultures observed during the same period of time. the type anitratus was the most common (79.4%) although there were no significant differences in epidemiology, sensitivity, or clinical significance between both types. only in 55.8% of the cases bacteremia was considered of clinical ... | 1990 | 2098121 |
| [nosocomial bacteremia caused by acinetobacter]. | forty episodes of nosocomial acinetobacter calcoaceticus bacteremia produced by the anitratus type over a period of 4 years were analyzed and compared with a control group of 28 patients with bacteremia produced by gram negative bacilli. although most of acinetobacter bacteremia were endemic an outbreak involving 12 cases were observed in an intensive care unit during the study period. thirteen patients presented a transient bacteremia. when the site of origin of the infection could be establish ... | 1990 | 2098118 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. research group for testing imipenem susceptibility on clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at 459 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were performed at each hospital laboratory and the tests were carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: , ++, + and -. ipm had significantly high activity against st ... | 1990 | 2086814 |
| in vitro activities of cefoperazone and sulbactam singly and in combination against cefoperazone-resistant members of the family enterobacteriaceae and nonfermenters. | among 28,000 isolates of the family enterobacteriaceae and nonfermenters isolated at multiple medical centers, 1,084 (4%) were resistant to cefoperazone (mic, greater than or equal to 64 micrograms/ml) and 1,711 (6%) exhibited cefoperazone mics of 2 to 32 micrograms/ml. ninety-six percent of these 2,795 isolates produced beta-lactamase, as determined by the nitrocefin test. sulbactam alone (8 micrograms/ml) was inactive against 99.6% of the isolates other than acinetobacter calcoaceticus and pse ... | 1990 | 2073118 |
| nucleotide sequence analysis reveals linked n-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of streptomyces hygroscopicus. | nucleotide sequence analysis of a 5,000-bp region of the bialaphos antibiotic production (bap) gene cluster defined five open reading frames (orfs) which predicted structural genes in the order bah, orf1, orf2, and orf3 followed by the regulatory gene, brpa (h. anzai, t. murakami, s. imai, a. satoh, k. nagaoka, and c.j. thompson, j. bacteriol. 169:3482-3488, 1987). the four structural genes were translationally coupled and apparently cotranscribed from an undefined promoter(s) under the positive ... | 1991 | 2066341 |
| cellular fatty acid composition of acinetobacter strains. | the cellular fatty acids of the different biotypes of acinetobacter calcoaceticus were determined by high resolution gas-liquid chromatography. while all strains exhibited, qualitatively, similar fatty acid compositions, quantitative differences were observed in strains belonging to defined phenotypic subgroups which can be used for differentiation. | 1991 | 2030649 |
| reliability of phenotypic tests for identification of acinetobacter species. | a numerical approach was used for identification of 198 acinetobacter strains assigned to dna groups according to the classification of tjernberg and ursing (i. tjernberg and j. ursing, apmis 97:595-605, 1989). the matrix used was constructed from data published by bouvet and grimont (p.j.m. bouvet and p.a.d. grimont, int. j. syst. bacteriol. 36:228-240, 1986) and bouvet and jeanjean (p.j.m. bouvet and s. jeanjean, res. microbiol. 140:291-299, 1989). the tests chosen were those of the simplified ... | 1991 | 2007635 |
| fatal septicaemia with selenomonas sputigena and acinetobacter calcoaceticus. a case report. | a 38-year-old man with a history of alcohol abuse developed rapidly fulminating septicaemia and died. selenomonas sputigena and acinetobacter calcoaceticus were isolated from a blood culture. selenomonas sputigena is a motile anaerobic gram-negative rod rarely associated with systemic disease. difficulties in isolation and taxonomic identification are discussed. | 1991 | 1993118 |
| immune response to acinetobacter calcoaceticus infection in man. | after growth in an iron-depleted chemically-defined medium acinetobacter calcoaceticus expressed four high mol. wt outer-membrane proteins (omps) which were repressed under iron supplementation or in a complex laboratory medium. immunoblotting with serum from a septicaemic patient infected with a. calcoaceticus revealed antibody binding to these iron-repressible omps, indicating that they were expressed in vivo, and also to the 42- and 18-kda omps. although the antibody response to the omps did ... | 1991 | 1990144 |
| comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in acinetobacter calcoaceticus and from the tol-plasmid-encoded toluene pathway in pseudomonas putida. n-terminal amino acid sequences, amino acid compositions and immunological cross-reactions. | 1. n-terminal sequences were determined for benzyl alcohol dehydrogenase, benzaldehyde dehydrogenase i and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus n.c.i.b. 8250, benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by the tol plasmid pww53 in pseudomonas putida mt53 and yeast k(+)-activated aldehyde dehydrogenase. comprehensive details of the sequence determinations have been deposited as supplementary publication sup 50161 (5 pages) at the british library d ... | 1991 | 1989592 |
| [epidemiologic study of pathogens causing nosocomial infections]. | nosocomial infection is a global problem. it significantly adds to the expected duration of hospitalization resulting with an increased economic burden and represents a leading cause of death. the characteristics of nosocomial infection vary from country to country and also changes with time. in order to understand the pathogens causing nosocomial infection in the large teaching hospital in taiwan, we collected and analysed the surveillence data of nosocomial infection at the national taiwan uni ... | 1990 | 1982123 |
| [a membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 3. inhibition of the enzyme]. | the alanine aminopeptidase from acinetobacter calcoaceticus is inhibited by sh-reagents like p-hydroxymercuribenzote, ellman's reagent, n-bromosuccinimide, and metal chelating agents like 1,10-phenanthroline. the aap is competitively inhibited by l-amino acids such as leucine, phenylalanine, and valine having hydrophobic side chains. bacitracin (ki = 2.0.10(-6) mol/l) inhibits aap stronger than puromycin (ki = 8.0.10(-6) mol/l). in contrast, the aeromonas aminopeptidase (ec 3.4.11.10) is stronge ... | 1990 | 1980190 |
| the survival of acinetobacter calcoaceticus inoculated on fingertips and on formica. | when inoculated on the fingers of three volunteers, strains of acinetobacter calcoaceticus var. anitratus survived better than strains of var. lwoffii; 60 min after an inoculum of 10(4) cfu/finger, washings yielded between 2.6 x 10(1) (for a sporadic strain of var. lwoffii) and 7.2 x 10(2) cfu/finger (for an epidemic strain of var. anitratus). all five test strains survived better on formica than on skin, and from an inoculum of 10(4) cfu, between 6.4 x 10(2) and 2.2 x 10(3) cfu/finger were reco ... | 1990 | 1971628 |
| in-vitro and in-vivo activities of sulbactam and ytr830h against acinetobacter calcoaceticus. | the antibacterial activities of sulbactam and ytr830h, both beta-lactamase inhibitors, were studied against acinetobacter calcoaceticus, in vitro and in vivo. in-vitro susceptibility tests showed both to be very active against 80 clinical isolates of a. calcoaceticus. against an intraperitioneal infection in mice, caused by a. calcoaceticus ac54, the 50% effective doses of sulbactam and ytr830h were 1.62 and 16.7 mg/kg, respectively. against a. calcoaceticus s31, a strain resistant to gentamicin ... | 1990 | 1964157 |
| a sheep survived for 48 days with the biventricular bypass type total artificial heart. | a biventricular bypass type total artificial heart (bvb-tah) utilizing two pusher-plate pumps was developed and implanted in a sheep for 48 days with excellent results. a hall effect sensor was utilized to operate each pump independently with a full stroke at variable rates (vr). with this system, the animal's hemodynamics was kept physiologically, and all metabolic parameters except hemoglobin and hematocrit returned to normal three weeks after implantation. however, signs of infection appeared ... | 1991 | 1962530 |
| [antibacterial activities of ofloxacin against recent isolates from patients with community-acquired infections]. | in order to survey antibacterial activities of ofloxacin (oflx) against 1,440 bacterial strains isolated from patients with community-acquired infections in 1987 and 1990, minimum inhibitory concentrations (mics) of the drug as well as those of other new quinolones and oral cephems were determined. the following conclusions were reached. 1. comparison of the mic distribution for strains isolated in 1987 with those in 1990 suggested a tendency toward an increase in the frequency of oflx-resistant ... | 1991 | 1960856 |
| association of contaminated gloves with transmission of acinetobacter calcoaceticus var. anitratus in an intensive care unit. | acinetobacter calcoaceticus var. anitratus is an important nosocomial pathogen that has been associated with environmental reservoirs. an increased isolation rate of a. anitratus in our intensive care units (icus), from 0.03% (two of 7,800) to 0.5% (seven of 1,300) (p less than 0.00003), prompted an investigation. | 1991 | 1951409 |
| microbial growth and endotoxin production in the intravenous anesthetic propofol. | in this study, we measured microbial growth and endotoxin production in the intravenous anesthetic propofol using 10 different microbial strains; 6 isolated from outbreak cases and 4 from laboratory stock cultures. | 1991 | 1940276 |
| potential dna slippage structures acquired during evolutionary divergence of acinetobacter calcoaceticus chromosomal benabc and pseudomonas putida tol pww0 plasmid xylxyz, genes encoding benzoate dioxygenases. | the xylxyz dna region is carried on the tol pww0 plasmid in pseudomonas putida and encodes a benzoate dioxygenase with broad substrate specificity. the dna sequence of the region is presented and compared with benabc, the chromosomal region encoding the benzoate dioxygenase of acinetobacter calcoaceticus. corresponding genes from the two biological sources share common ancestry: comparison of aligned xylx-bena, xyly-benb, and xylz-benc amino acid sequences revealed respective identities of 58.3, ... | 1991 | 1938949 |
| purification and characterization of acinetobacter calcoaceticus isocitrate lyase. | acinetobacter calcoaceticus is capable of growing on acetate or compounds that are metabolized to acetate. during adaptation to growth on acetate, a. calcoaceticus b4 exhibits an increase in nadp(+)-isocitrate dehydrogenase and isocitrate lyase activities. in contrast, during adaptation to growth on acetate, escherichia coli exhibits a decrease in nadp(+)-isocitrate dehydrogenase activity that is caused by reversible phosphorylation of specific serine residues on this enzyme. also, in e. coli, i ... | 1991 | 1938889 |
| imipenem/cilastatin as secondary therapy for bacterial infections. | imipenem/cilastatin (ipm/cs) was used to treat 39 documented infections in patients who had failed to respond to other antibiotic regimens. the overall response rating was 76.9%. respiratory infections responded less frequently (efficacy rating, 55.6%) to ipm/cs than abdominal infections, urinary tract infections, or sepsis. methicillin-resistant staphylococcus aureus, xanthomonas maltophilia, and acinetobacter calcoaceticus were less sensitive to ipm/cs therapy than the other bacterial strains ... | 1991 | 1933995 |
| [antimicrobial activity of cefpiramide to fresh clinical isolates]. | in the subjects of 835 strains of 37 clinically isolated microbial strains, which were separated and identified among materials collected from patients with various infections and which were sent from medical therapeutic institutions throughout japan in 1990, for the purpose of examining the antimicrobial activity of cefpiramide (cpm), its minimum inhibitory concentration (mic), together with those of other cephem antibiotics, was determined, and the following conclusions were obtained. 1. micro ... | 1991 | 1920808 |
| community acquired pneumonia: aetiology and prognostic index evaluation. | a 10 month prospective study of all adults admitted to waikato hospital with community acquired pneumonia was performed to assess aetiology, mortality, hospital stay, and the value of a prognostic index based on that obtained from a british thoracic society study. the 92 patients in the survey had a mean age of 56 (range 13-97) years. a microbiological diagnosis was established in 72%; streptococcus pneumoniae (33%), mycoplasma pneumoniae (18%), and influenza a virus (8%) were the most common mi ... | 1991 | 1907034 |
| in vitro and in vivo evaluation of ro 09-1428, a new parenteral cephalosporin with high antipseudomonal activity. | ro 09-1428, a new parenteral cephalosporin with a catechol moiety attached at position 7 of the cephalosporin ring, showed high in vitro activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, and streptococcus pyogenes, with mics for 90% of strains tested (mic90s) of less than or equal to 0.39 micrograms/ml. morganella morganii, providencia rettgeri, citrobacter freundii, haemophilus influenzae, staphylococcus aureus, and streptococcus pneumoniae were inhi ... | 1991 | 1906261 |
| nucleotide sequences of the acinetobacter calcoaceticus benabc genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases. | the nucleotide sequences of the acinetobacter calcoaceticus benabc genes encoding a multicomponent oxygenase for the conversion of benzoate to a nonaromatic cis-diol were determined. the enzyme, benzoate 1,2-dioxygenase, is composed of a hydroxylase component, encoded by benab, and an electron transfer component, encoded by benc. comparison of the deduced amino acid sequences of benabc with related sequences, including those for the multicomponent toluate, toluene, benzene, and naphthalene 1,2-d ... | 1991 | 1885518 |
| susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to newer antimicrobials. | the susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to a variety of newer antimicrobials was determined. sixty-three amikacin-resistant isolates were collected from 61 inpatients at a tertiary-care hospital between 1985 and 1988. the organisms included xanthomonas maltophilia (24 isolates), pseudomonas aeruginosa (16), pseudomonas species (3), serratia marcescens (10), flavobacterium meningosepticum (4), enterobacter species (3), acinetobacter calcoaceticu ... | 1991 | 1860303 |
| [intrahospital septicemia due to acinetobacter calcoaceticus var. anitratus in a neonatal intensive care unit]. | twenty-one cases of sepsis by acinetobacter calcoaceticus biovar anitratus occurred, ten in 1989 and eleven in 1990 (january-april). in this last year, there were nine cases with characteristics of an epidemic outbreak. it was noted that sepsis was predominant in males, babies of less than 2,500 g and less than 37 weeks of gestation. they were hospitalized in our intensive care unit for different problems, but almost all had mechanical ventilation, parenteral nutrition and received antibiotics p ... | 1991 | 1844938 |
| [acinetobacter in a metropolitan neonatal unit: clinical and microbiological aspects]. | acinetobacter calcoaceticus var. anitratus (aca) was isolated from two newborn infants and var. iwoffi of the same species from a third patient within a month in the neonatal unit of a general hospital at metropolitan santiago, chile. the first isolate was obtained from a neonate with infection of the skin surrounding a colostomy operation after surgery for tracheo esophageal fistulae, in march 1989. the second case's skin became colonized after he was admitted for surgical repair of severe gast ... | 1991 | 1844163 |
| [sensitivity of bacteria of the genus acinetobacter to antibiotics and ofloxacin]. | between 1989-1989 276 strains of acinetobacter genus were isolated which contained: acinetobacter calcoaceticus subsp. anitratus (n = 167), acinetobacter calcoaceticus subsp. iwoffi (n- = 83), acinetobacter haemolyticus (n = 26). their sensitivity to aminoglycoside antibiotics, beta-lactams, tetracyclines, chloramphenicol, colistin, and ofloxacin was tested. more than 90% of strains were sensitive to colistin and ofloxacin. the sensitivity to remaining antibiotics differentiated depending on spe ... | 1991 | 1823372 |
| mechanisms of resistance to beta-lactam antibiotics in acinetobacter calcoaceticus. | examination of 12 strains of acinetobacter calcoaceticus revealed that the strains expressed different constitutive levels of beta-lactamase. mutants resistant to cefoxitin, cefoperazone or ceftazidime were selected from a strain producing a low level of beta-lactamase. all the mutants showed no change in expression of beta-lactamase, but produced penicillin-binding proteins with altered expression and/or affinity for beta-lactams. in addition, the outer membrane of the mutants showed decreased ... | 1991 | 1816177 |
| [imipenem-cilastatin cure of meningitis caused by acinetobacter calcoaceticus]. | 1991 | 1805958 | |
| [effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections]. | the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ... | 1991 | 1805697 |
| [sensitivity of acinetobacter calcoaceticus-baumanni complex and acinetobacter lwoffi to common antibiotics]. | 1991 | 1799617 | |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ... | 1991 | 1798067 |
| release of transforming plasmid and chromosomal dna from two cultured soil bacteria. | the release of chromosomal and plasmid dna from acinetobacter calcoaceticus and bacillus subtilis cultivated in minimal medium and broth over a period of 50 h was monitored and related to growth phase, autolysis, dnase production and natural competence. the released dnas were biologically active in natural transformation. in addition, the circular integrity of a released b. subtilis shuttle vector (phv14) was demonstrated by artificial transformation of escherichia coli. in cultures of both stra ... | 1991 | 1793338 |
| [quantitation of acinetobacter calcoaceticus in mixed bacterial cultures by an enzyme immunoassay]. | an enzyme-linked immunosorbent assay using polyclonal antibodies from rabbits has been developed for quantification of acinetobacter calcoaceticus. bacteria were added to the wells of a microtiter plate coated with anti-acinetobacter immunoglobulin. for detecting bound cells the peroxidase-labelled immunoglobulin fraction was used. over a distinct range there is a linear correlation between bound bacteria and measured absorbance allowing a quantification of bacteria in an order from 10(7) to 10( ... | 1991 | 1793089 |
| use of a broad-host-range gyra plasmid for genetic characterization of fluoroquinolone-resistant gram-negative bacteria. | the gyra genotypes of ciprofloxacin-resistant clinical isolates of escherichia coli (n = 3), klebsiella pneumoniae (n = 4), providencia stuartii (n = 2), pseudomonas aeruginosa (n = 1), and acinetobacter calcoaceticus (n = 1) were analyzed in a dominance test. this test is based on the dominance of a wild-type gyra gene (gyra+) over the quinolone resistance allele (gyra) in a heterodiploid strain. plasmid pbp515, developed to carry the gyra+ gene of e. coli k-12 on a broad-host-range vector deri ... | 1991 | 1759823 |
| cis-diol dehydrogenases encoded by the tol pww0 plasmid xyll gene and the acinetobacter calcoaceticus chromosomal bend gene are members of the short-chain alcohol dehydrogenase superfamily. | in the aerobic degradation of benzoate by bacteria, benzoate is first dihydroxylated by a ring-hydroxylating dioxygenase to form a cis-diol (1,2-dihydroxycyclohexa-3,4-diene carboxylate) which is subsequently transformed to a catechol by an nad(+)-dependent cis-diol dehydrogenase. the structural gene for this dehydrogenase, encoded on tol plasmid pww0 of pseudomonas putida (xyll) and that encoded on the chromosome of acinetobacter calcoaceticus (bend), were sequenced. they encode polypeptides of ... | 1992 | 1740120 |