Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| analysis of a lea gene promoter via agrobacterium-mediated transformation of the desiccation tolerant plant lindernia brevidens. | an agrobacterium tumefaciens-based transformation procedure was developed for the desiccation tolerant species lindernia brevidens. leaf explants were infected with a. tumefaciens strain gv3101 harbouring a binary vector that carried the hygromycin resistance gene and an egfp reporter gene under the control of a native dehydration responsive lea promoter (lb2745pro). pcr analysis of the selected hygromycin-resistant plants revealed that the transformation rates were high (14/14) and seeds were o ... | 2007 | 17497152 |
| diverse bacteria isolated from root nodules of trifolium, crotalaria and mimosa grown in the subtropical regions of china. | to analyze the diversity and relationships of rhizobia in the subtropical and tropical zones of china, we characterized 67 bacterial strains isolated from root nodules of five legume species in the genera trifolium, crotalaria and mimosa . pcr-amplified 16s rdna rflp, numerical taxonomy, sds-page of whole cell proteins, sequencing of 16s rdna and dna-dna hybridization grouped the isolates into 17 lineages belonging to bradyrhizobium, mesorhizobium, rhizobium, sinorhizobium and burkholderia, as w ... | 2007 | 17497134 |
| bioformulation of burkholderia sp. mssp with a multispecies consortium for growth promotion of cajanus cajan. | the present work was undertaken to formulate an effective bioformulation using burkholderia sp. strain mssp, a known plant-growth-promoting rhizobacterium. mssp was tagged with the reporter gene of green fluorescent protein (gfp) to monitor its population in cost-effective solid carriers, including sugarcane-bagasse, sawdust, cocoa peat, rice husk, wheat bran, charcoal, and rock phosphate, and paneer-whey as liquid carrier. physical and chemical properties of different low-cost carrier materials ... | 2007 | 17496969 |
| agrobacterium-mediated insertional mutagenesis of the ochratoxigenic fungus aspergillus westerdijkiae. | aspergillus westerdijkiae is a potent ochratoxin a (ota) producer that has been found in coffee beans. ota is known to have nephrotoxic effects and carcinogenic potential in animal species. here we report for the first time the agrobacterium-mediated transformation for aspergillus westerdijkiae and the generation of ochratoxin-defective mutants. conidia were transformed to hygromycin b resistance using strain agl-1 of agrobacterium tumefaciens. the obtained transformation frequency was up to 47 ... | 2007 | 17496961 |
| arabidopsis vire2 interacting protein2 is required for agrobacterium t-dna integration in plants. | agrobacterium tumefaciens-mediated genetic transformation is an efficient tool for genetic engineering of plants. vire2 is a single-stranded dna binding agrobacterium protein that is transported into the plant cell and presumably protects the t-dna from degradation. using a yeast two-hybrid system, we identified arabidopsis thaliana vire2-interacting protein2 (vip2) with a not domain that is conserved in both plants and animals. furthermore, we provide evidence supporting vip2 interaction with v ... | 2007 | 17496122 |
| high efficiency poplar transformation. | with the completion of the poplar tree genome database, populus species have become one of the most useful model systems for the study of woody plant biology. populus tremuloides (quaking aspen) is the most wide-spread tree species in north america, and its rapid growth generates the most abundant wood-based biomass out of any other plant species. to study such beneficial traits, there is a need for easier and more efficient transformation procedures that will allow the study of large numbers of ... | 2007 | 17492451 |
| characterization of agrobacterium tumefaciens dna ligases c and d. | agrobacterium tumefaciens encodes a single nad+-dependent dna ligase and six putative atp-dependent ligases. two of the ligases are homologs of ligd, a bacterial enzyme that catalyzes end-healing and end-sealing steps during nonhomologous end joining (nhej). agrobacterium ligd1 and atuligd2 are composed of a central ligase domain fused to a c-terminal polymerase-like (pol) domain and an n-terminal 3'-phosphoesterase (pe) module. both ligd proteins seal dna nicks, albeit inefficiently. the ligd2 ... | 2007 | 17488851 |
| auxin and nitric oxide control indeterminate nodule formation. | rhizobia symbionts elicit root nodule formation in leguminous plants. nodule development requires local accumulation of auxin. both plants and rhizobia synthesise auxin. we have addressed the effects of bacterial auxin (iaa) on nodulation by using sinorhizobium meliloti and rhizobium leguminosarum bacteria genetically engineered for increased auxin synthesis. | 2007 | 17488509 |
| production and composition of extracellular polysaccharide synthesized by a rhizobium isolate of vigna mungo (l.) hepper. | an extracellular polysaccharide (eps) was produced by a rhizobium sp. isolated from the root nodules of vigna mungo (l.) hepper. maximum eps production (346 mg l(-1)) was when the yeast extract basal medium was supplemented with mannitol (1%), biotin (1.5 mg l(-1)) and asparagine (0.3%). ribose (53%) and mannose (47%) were the principle monomers of the eps. chemical, chromatographic and spectroscopic analysis showed that this polymer, which has man(4)rib(1) as an oligomeric subunit, has an appar ... | 2007 | 17487547 |
| rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. | expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in cell biology. the protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest. these cells can be used to generate stable plant lines using a more time-consuming, cell culture technique ... | 2006 | 17487191 |
| [tolc mutant of sinorhizobium meliloti strain skhm1-188 fails to establish effective symbiosis with alfalfa]. | the tolc mutant tr63 of sinorhizobium meliloti was generated by random tn5 mutagenesis in the effective strain skhm1-188. the mutant did not produce fluorescent halos in uv light on the lb medium containing calcofluor white, which suggests that modification occurred in the production of exopolysaccharide eps1. mutant tr63 also manifested nonmucoidness both on minimal and low-phosphate mops media, and this was most likely connected with the absence of the second exopolysaccharide of s. meliloti ( ... | 2007 | 17486750 |
| expression of human acidic fibroblast growth factor in nicotiana benthamiana with a potato-virus-x-based binary vector. | the afgf (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. a dna fragment encoding hafgf (human afgf) has been cloned into the pvx (potato virus x)-based binary vector (pgr107) and transiently expressed in nicotiana benthamiana (a wild australian tobacco) by agroinfection. approx. 1 week after agroinfection, the recombinant hafgf accumulated in the agroinfected plants reached up to 1% of the ... | 2007 | 17484724 |
| fungal genomics goes industrial. | 2007 | 17483839 | |
| revegetation of a lakeside barren area by the application of plant growth-promoting rhizobacteria. | the growth stimulation of wild plants by several bacterial species showing plant growth-promoting capabilities was examined in a barren lakeside area at lake paro, korea. microbial numbers and activities in the field soil were monitored for 73 days after inoculation of the bacteria. the acridine orange direct counts for the total soil bacterial populations ranged between 2.0-2.3x10(9) cells/g soil and 1.4-1.8x10(9) cells/g soil in the inoculated and uninoculated soils, respectively. the numbers ... | 2007 | 17483804 |
| insect-microbe mutualism without vertical transmission: a stinkbug acquires a beneficial gut symbiont from the environment every generation. | the broad-headed bug riptortus clavatus (heteroptera: alydidae) possesses a number of crypts at a posterior midgut region, which house a dense population of a bacterial symbiont belonging to the genus burkholderia. although the symbiont is highly prevalent (95 to 100%) in the host populations, the symbiont phylogeny did not reflect the host systematics at all. in order to understand the mechanisms underlying the promiscuous host-symbiont relationship despite the specific and prevalent associatio ... | 2007 | 17483286 |
| 28-homobrassinolide protects chickpea (cicer arietinum) from cadmium toxicity by stimulating antioxidants. | in the present experiment the seeds of cicer arietinum (l.) cv. uday were inoculated with specific rhizobium grown in sandy loam soil and were allowed to grow for 15 days. at this stage, the seedlings were supplied with 0, 50, 100 or 150 microm of cadmium in the form of cadmium chloride and sprayed with 0.01 microm of 28-homobrassinolide (hbl) at 30-day stage. the data indicated that plant fresh and dry mass, number of nodules, their fresh and dry mass, leghemoglobin content, nitrogen and carboh ... | 2008 | 17481788 |
| controlling the sucrose concentration increases coenzyme q10 production in fed-batch culture of agrobacterium tumefaciens. | the production yield of coenzyme q(10) (coq(10)) from the sucrose consumed by agrobacterium tumefaciens kccm 10413 decreased, and high levels of exopolysaccharide (eps) accumulated after switching from batch culture to fed-batch culture. therefore, we examined the effect of sucrose concentration on the fermentation profile by a. tumefaciens. in the continuous fed-batch culture with the sucrose concentration maintained constantly at 10, 20, 30, and 40 g l(-1), the dry cell weight (dcw), specific ... | 2007 | 17479258 |
| genetic diversity of rhizobia associated with vicia faba in three ecological regions of china. | great genetic diversity was revealed among 75 rhizobal isolates associated with vicia faba grown in chinese fields with aflp, ardra, 16s rdna sequencing, dna-dna hybridization, box-pcr and rflp of pcr-amplified nodd and nodc. most of the isolates were rhizobium leguminosarum, and six isolates belonged to an unnamed rhizobium species. in the homogeneity analysis, the isolates were grouped into three clusters corresponding to (1) autumn sowing (subtropical) region where the winter ecotype of v. fa ... | 2007 | 17479251 |
| [active oxygen species in pea seedlings during the interactions with symbiotic and pathogenic microorganisms]. | the level of active oxygen species (aos)--superoxide anion radical (o2*-) and hydrogen peroxide (h2o2)--in pea (pisum sativum l.) cultivar marat seedlings was studied upon their inoculation with symbiotic (rhizobium leguminosarum bv. viceae strain ciam 1026) and pathogenic (pseudomonas syringae pv. pisi sackett) microorganisms. different patterns of the changes in aos in pea seedlings during the interactions with the symbiont and the phytopathogen were recorded. it is assumed that o2*- and h2o2 ... | 2007 | 17476814 |
| [a study of the competitive properties of the aluminum-tolerant strain rhizobium leguminosarum bv. trifolii 9-4a by the antibiotic resistance method]. | the virulence, competitive ability, and symbiotic efficiency of 2 rhizobium leguminosarum bv. trifolii strains--the wild aluminum tolerant strain 9-4a and the commercial strain 348a-were compared when introducing their variants marked with antibiotic resistance into the rhizosphere of red clover (trifolium pratense l.) plants. high virulence and competitive ability of the strain tolerant to aluminum was demonstrated by a concurrent inoculation of the seeds with these two strains. the resistance ... | 2007 | 17476807 |
| molecular basis of transcriptional antiactivation. tram disrupts the trar-dna complex through stepwise interactions. | conjugative transfer of agrobacterium ti plasmids is regulated by trar, a quorum-sensing activator. quorum dependence requires tram, which binds to and inactivates trar. in this study, we showed that trar and tram form a 151-kda stable complex composed of two trar and two tram dimers both in vitro and in vivo. when interacted with trar bound to tra box dna, wild-type tram formed a nucleoprotein complex of 77 kda composed of one dimer of each protein and dna. the complex converted to the 151-kda ... | 2007 | 17475619 |
| rational design of inhibitors of vira-virg two-component signal transduction. | vira-virg two-component system regulates the vir (virulence) operon in response to specific host factors (xenognosins) in the plant pathogen agrobacterium tumefaciens. using whole cell assays, stable inhibitors inspired by the labile natural benzoxazinone inhibitor hdmboa are developed. it is found that aromatic aldehydes represent a minimal structural unit for activity. in particular, 3-hydroxy-4,6-dimethoxy-3h-isobenzofuran-1-one (hdi) was found to have the highest activity, making it the most ... | 2007 | 17470391 |
| novel scar primers for specific and sensitive detection of agrobacterium vitis strains. | an agrobacterium vitis-specific dna fragment (pavs3) was generated from pcr polymorphic bands amplified by primer urp 2r. a. vitis specificity of this fragment was confirmed by southern hybridization with genomic dna from different agrobacterium species. sequence-characterized amplified region (scar) markers were developed for a. vitis specific detection, using 24-mer oligonucleotide primers designed from the flanking ends of the 670bp insert in pavs3. the scar primers amplified target sequences ... | 2009 | 17467252 |
| sequence analysis of the 181-kb accessory plasmid psmesm11b, isolated from a dominant sinorhizobium meliloti strain identified during a long-term field release experiment. | the 181 251 bp accessory plasmid psmesm11b of sinorhizobium meliloti strain sm11, belonging to a dominant indigenous s. meliloti subpopulation identified during a long-term field release experiment, was sequenced. this plasmid has 166 coding sequences (cdss), 42% of which encode proteins with homology to proteins of known function. plasmid psmesm11b is a member of the repabc replicon family and contains a large gene region coding for a conjugation system similar to that of other self-transmissib ... | 2007 | 17466030 |
| environmental fate of endocrine-disrupting dimethyl phthalate esters (dmpe) under sulfate-reducing condition. | dimethyl phthalate esters (dmpe) can easily be released into the environment from plastic products. as endocrine disruptors, dmpe mimic estrogenic activities in animals and humans. the metabolites of dmpe are suspected to cause even more serious health problems. among the common sterilization techniques adopted in the study of dmpe degradation, the average loss of the parent dmpe compounds after autoclaving was as high as 21.26%. in contrast, the loss after 0.2 microm filtration was significantl ... | 2007 | 17462710 |
| exor is genetically coupled to the exos-chvi two-component system and located in the periplasm of sinorhizobium meliloti. | sinorhizobium meliloti enters into a symbiotic relationship with legume host plants, providing fixed nitrogen in exchange for carbon and amino acids. in s. meliloti, exor and the exos-chvi two-component system regulate the biosynthesis of succinoglycan, an exopolysaccharide important for host invasion. it was previously reported that a loss-of-function mutation in exor and a gain-of-function mutation in exos cause overproduction of succinoglycan and loss of motility, indicating that exor negativ ... | 2007 | 17462014 |
| mutagenesis of the carboxy terminal protease ctpa decreases desiccation tolerance in rhizobium leguminosarum. | to better understand the role of proteases in rhizobium leguminosarum biovar viciae, a gene with homology to the carboxy-terminal protease (ctpa), which belongs to a novel group of serine proteases, was studied. the ctpa gene was cloned and mutated using allelic exchange and a gusa reporter gene was used to study ctpa expression. mutational analysis shows that ctpa is critical for the viability of r. leguminosarum when cells are grown on complex semi-solid media but is dispensable when cells are ... | 2007 | 17456188 |
| a diagnosis-prescription system for nitrogen management in environment. | a decision support system in the framework of the geographic information system (gis) and subsurface flow model, hydrosub, were used to identify critical areas from simulated spatial distributions of relative nitrogen export. diagnosis and prescription expert systems (es) are developed and applied to the identification of probable causes of excessive nitrogen export and selection of appropriate best management practices (bmps). the result is a spatially distributed set of recommended best manage ... | 2007 | 17454363 |
| agrobacterium-mediated transformation of the dwarf pomegranate (punica granatum l. var. nana). | the dwarf pomegranate (punica granatum l. var. nana) is a dwarf ornamental plant that has the potential to be the model plant of perennial fruit trees because it bears fruits within 1 year of seedling. we established an agrobacterium-mediated transformation system for the dwarf pomegranate. adventitious shoots regenerated from leaf segments were inoculated with a. tumefaciens strain eha105 harboring the binary vector pbin19-sgfp, which contains neomycin phosphotransferase (npt ii) and green fluo ... | 2007 | 17453216 |
| salt and drought tolerance of transgenic salvia miltiorrhiza bunge with the talea1 gene. | talea1, a gene encoding a late-embryogenesis-abundant protein, was cloned from wheat and was transformed into salvia miltiorrhiza bunge by agrobacterium-mediated leaf-disk transformation method. seven transgenic lines were obtained after kanamycin (50 mg/l) screening. six positive lines were obtained by pcr amplification, and after four additional generations, one stable line was obtained by southern hybridization. transgenic plants had better growth states than control plants on mediums contain ... | 2007 | 17452795 |
| mesorhizobium spp. are the main microsymbionts of caragana spp. grown in liaoning province of china. | caragana species are woody legumes widely distributed in the arid regions of china. these plants form root nodules but their nodule bacteria have not been clearly classified. a total of 112 symbiotic bacterial isolates were obtained from four caragana species grown in liaoning province and were characterized with ardra, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, box-pcr and sequencing of the 16s rrna gene. most of them were classified as mesorhizobium belonging to 11 ... | 2007 | 17451445 |
| trehalose biosynthesis in rhizobium leguminosarum bv. trifolii and its role in desiccation tolerance. | rhizobium leguminosarum bv. trifolii forms nitrogen-fixing root nodules on the pasture legume trifolium repens, and t. repens seed is often coated with a compatible r. leguminosarum bv. trifolii strain prior to sowing. however, significant losses in bacterial viability occur during the seed-coating process and during storage of the coated seeds, most likely due to desiccation stress. the disaccharide trehalose is known to function as an osmoprotectant, and trehalose accumulation due to de novo b ... | 2007 | 17449695 |
| rhizavidin from rhizobium etli: the first natural dimer in the avidin protein family. | rhizobium etli cfn42 is a symbiotic nitrogen-fixing bacterium of the common bean phaseolus vulgaris. the symbiotic plasmid p42d of r. etli comprises a gene encoding a putative (strept)avidin-like protein, named rhizavidin. the amino acid sequence identity of rhizavidin in relation to other known avidin-like proteins is 20-30%. the amino acid residues involved in the (strept)avidin-biotin interaction are well conserved in rhizavidin. the structural and functional properties of rhizavidin were car ... | 2007 | 17447892 |
| a rhizobia strain isolated from root nodule of gymnosperm podocarpus macrophyllus. | symbiotic nitrogen fixation of rhizobia and leguminous plants is considered as the most important biologic nitrogen fixation system on earth. symbiotic nodulation of gymnosperm podocarpus macrophyllus and rhizobia has never been reported. in this study, 11 endophytic bacteria strains were isolated from root nodules of p. macrophyllus and its variation p. macrophyllus var. maki. the plant infection tests on these strains indicated that the isolated strains could be nodulated on p. macrophyllus pl ... | 2007 | 17447030 |
| agrobacterium rhizogenes-mediated transformation of soybean to study root biology. | this protocol is used to induce transgenic roots on soybean to study the function of genes required in biological processes of the root. young seedlings with unfolded cotyledons are infected at the cotyledonary node and/or hypocotyl with agrobacterium rhizogenes carrying the gene construct to be tested and the infection sites are kept in an environment of high humidity. when the emerged hairy roots can support the plants, the main roots are removed and the transgenic roots can be tested. using t ... | 2007 | 17446894 |
| expression of human nuclear receptors in plants for the discovery of plant-derived ligands. | plants have the potential to produce a wide array of secondary metabolites that have utility as drugs to treat human diseases. to tap this potential, functional human nuclear receptors have been expressed in plants to create in planta screening assays as a tool to discover natural product ligands. assays have been designed and validated using 3 nuclear receptors: the estrogen receptor (er), the androgen receptor (ar), and the heterodimeric retinoid x receptor-alpha plus thyroid hormone receptor- ... | 2007 | 17438068 |
| the hopx (avrpphe) family of pseudomonas syringae type iii effectors require a catalytic triad and a novel n-terminal domain for function. | many gram-negative plant pathogenic bacteria employ type iii secretion systems to deliver effector proteins directly into the host cell during infection. on susceptible hosts, type iii effectors aid pathogen growth by manipulating host defense pathways. on resistant hosts, some effectors can activate specific host disease resistance (r) genes, leading to generation of rapid and effective immune responses. the biochemical basis of these processes is poorly understood. the hopx (avrpphe) family is ... | 2007 | 17427805 |
| a simplified method for the analysis of transcription factor-promoter interactions that allows high-throughput data generation. | transient expression systems are intensively used to study the transactivation potential of transcription factors and to confirm target promoters. here we present a novel system based on the high-efficiency transformation of cultured arabidopsis thaliana cells by agrobacteria. to demonstrate the potential of this system, we compared it with a commonly used protoplast transfection assay, and studied the regulation of phenylpropanoid biosynthetic pathway genes by various transcription factors. bot ... | 2007 | 17425717 |
| agrobacterium-mediated viral vector-amplified transient gene expression in nicotiana glutinosa plant tissue culture. | a viral vector based on the bean yellow dwarf virus was investigated for its potential to increase transient gene expression. an intron-containing gus reporter gene and the cis-acting viral regulatory elements were incorporated in the viral vector and could be complemented by the viral replication-associated proteins provided on a secondary vector. all vectors were delivered to nicotiana glutinosa plant cell suspension or hairy root cultures via auxotrophic agrobacterium tumefaciens. cell cultur ... | 2007 | 17425328 |
| rhizobium etli ce3 bacteroid lipopolysaccharides are structurally similar but not identical to those produced by cultured ce3 bacteria. | rhizobium etli ce3 bacteroids were isolated from phaseolus vulgaris root nodules. the lipopolysaccharide (lps) from the bacteroids was purified and compared with the lps from laboratory-cultured r. etli ce3 and from cultures grown in the presence of anthocyanin. comparisons were made of the o-chain polysaccharide, the core oligosaccharide, and the lipid a. although lps from ce3 bacteria and bacteroids are structurally similar, it was found that bacteroid lps had specific modifications to both th ... | 2007 | 17420254 |
| root hair curling and rhizobium infection in medicago truncatula are mediated by phosphatidylinositide-regulated endocytosis and reactive oxygen species. | the symbiotic relationships between legumes and rhizobacteria involve extensive signalling between the two organisms. studies using genetic, biochemical, and pharmacological approaches have demonstrated the involvement of calcium and reactive oxygen species in the establishment of symbiotic interactions. in the early stage of the interactions rhizobia grow as infection thread within host root hairs and are internalized into the plant cells via endocytosis. it is shown here that inoculation of me ... | 2007 | 17420174 |
| monitoring circadian rhythms in arabidopsis thaliana using luciferase reporter genes. | arabidopsis thaliana is internationally adopted as the model plant species for molecular genetics. as such a huge range of resources are available for its study. arabidopsis does not display any obvious circadian rhythms; however, rhythms in gene expression can be readily detected. for this reason the promoters of rhythmically expressed genes have been fused to the firefly luciferase gene. using this reporter gene we have developed a number of automated techniques for monitoring luciferase activ ... | 2007 | 17417007 |
| replication-independent long-distance trafficking by viral rnas in nicotiana benthamiana. | viruses with separately encapsidated genomes could have their genomes introduced into different leaves of a plant, thus necessitating long-distance trafficking of the viral rnas for successful infection. to examine this possibility, individual or combinations of genome segments from the tripartite brome mosaic virus (bmv) were transiently expressed in leaves of nicotiana benthamiana plants using engineered agrobacterium tumefaciens. bmv rna3 was found to traffic from the initial site of expressi ... | 2007 | 17416731 |
| nitrogen fixation control under drought stress. localized or systemic? | legume-rhizobium nitrogen fixation is dramatically affected under drought and other environmental constraints. however, it has yet to be established as to whether such regulation of nitrogen fixation is only exerted at the whole-plant level (e.g. by a systemic nitrogen feedback mechanism) or can also occur at a local nodule level. to address this question, nodulated pea (pisum sativum) plants were grown in a split-root system, which allowed for half of the root system to be irrigated at field ca ... | 2007 | 17416644 |
| antitumor and antibacterial activity of ethylacetate extract of ludwigia hyssopifolia linn and its active principle piperine. | an alkaloid constituent 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine, trivial name piperine was isolated from ludwigia hyssopifolia linn. (family-onagraceae). the ethylacetate extract of the plant and the isolated compound piperine were studied for antitumor and in vitro antibacterial activity. ethylacetate extract showed 73.05 and 84.14% inhibition of agrobacterium tumefaciens-induced crown gall tumor formation in potato disc. piperine exhibited antitumor activity with ic50 valu ... | 2007 | 17416568 |
| regioselective and enzymatic production of gamma-resorcylic acid from resorcinol using recombinant escherichia coli cells expressing a novel decarboxylase gene. | a recombinant escherichia coli, expressing the rdc gene, which encodes a gamma-resorcylic acid decarboxylase (rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from rhizobium radiobacter wu-0108, converted 20 mm resorcinol to gamma-resorcylic acid with a 44% (mol/mol) yield at 30 degrees c for 7 h. the recombinant e. coli cells were recyclable at least five times for use as biocatalysts. | 2007 | 17415666 |
| optimization of human papillomavirus type 16 (hpv-16) l1 expression in plants: comparison of the suitability of different hpv-16 l1 gene variants and different cell-compartment localization. | virus-like particle-based vaccines for high-risk human papillomaviruses (hpvs) appear to have great promise; however, cell culture-derived vaccines will probably be very expensive. the optimization of expression of different codon-optimized versions of the hpv-16 l1 capsid protein gene in plants has been explored by means of transient expression from a novel suite of agrobacterium tumefaciens binary expression vectors, which allow targeting of recombinant protein to the cytoplasm, endoplasmic re ... | 2007 | 17412974 |
| a protocol for agrobacterium-mediated transformation in rice. | agrobacterium-mediated transformation of rice is an important method that has been widely adopted by many laboratories. however, because current approaches rely on culture systems, routine protocols have been established only in japonica rice, especially those varieties with higher regeneration potential. some very efficient methods have been developed for japonica varieties that enable high-throughput functional analysis in rice; however, many elite japonica, and most indica, varieties are diff ... | 2006 | 17406537 |
| genetic transformation of tobacco nt1 cells with agrobacterium tumefaciens. | this protocol is used to produce stably transformed tobacco (nicotiana tabacum) nt1 cell lines, using agrobacterium tumefaciens-mediated dna delivery of a binary vector containing a gene encoding hepatitis b surface antigen and a gene encoding the kanamycin selection marker. the nt1 cultures, at the appropriate stage of growth, are inoculated with a. tumefaciens containing the binary vector. a 3-day cocultivation period follows, after which the cultures are rinsed and placed on solid selective m ... | 2006 | 17406390 |
| agrobacterium-mediated transformation of arabidopsis thaliana using the floral dip method. | collective efforts of several laboratories in the past two decades have resulted in the development of various methods for agrobacterium tumefaciens-mediated transformation of arabidopsis thaliana. among these, the floral dip method is the most facile protocol and widely used for producing transgenic arabidopsis plants. in this method, transformation of female gametes is accomplished by simply dipping developing arabidopsis inflorescences for a few seconds into a 5% sucrose solution containing 0 ... | 2006 | 17406292 |
| interaction of an ihf-like protein with the rhizobium etli nifa promoter. | the nifa gene fulfills an essential role in the regulation of nitrogen fixation genes in rhizobium etli. transcription analysis of the nifa gene, assessed using promoter deletions, indicated an oxygen-independent expression, threefold higher during symbiosis as compared with free-living conditions. electrophoretic mobility shift assays using those nifa promoter deletion fragments, which were actively transcribed, demonstrated the specific interaction with r. etli cellular protein(s) resulting in ... | 2007 | 17403047 |
| ubiquinone-10 production using agrobacterium tumefaciens dps gene in escherichia coli by coexpression system. | ubiquinone (coenzyme q; abbreviation, uq) acts as a mobile component of the respiratory chain by playing an essential role in the electron transport system, and has been widely used in pharmaceuticals. the biosynthesis of uq involves 10 sequential reactions brought about by various enzymes. in this study we have cloned, expressed the decaprenyl diphosphate synthase, designated dps gene, from agrobacterium tumefaciens, and succeeded in detecting uq-10 in addition to innate uq-8 in escherichia col ... | 2007 | 17401144 |
| sensitive whole-cell biosensor suitable for detecting a variety of n-acyl homoserine lactones in intact rhizosphere microbial communities. | to investigate quorum sensing in rhizosphere soil, a whole-cell biosensor, agrobacterium tumefaciens(pahl-ice), was constructed. the biosensor responded to all n-acyl homoserine lactones (ahls) tested, except c(4) homoserine lactone, with a minimum detection limit of 10(-12) m, as well as to both exogenously added ahls and ahl-producing bacterial strains in soil. this highly sensitive biosensor reveals for the first time the increased ahl availability in intact rhizosphere microbial communities ... | 2007 | 17400771 |
| genetic determinants of swarming in rhizobium etli. | swarming motility is considered to be a social phenomenon that enables groups of bacteria to move coordinately atop solid surfaces. the differentiated swarmer cell population is embedded in an extracellular slime layer, and the phenomenon has previously been linked with biofilm formation and virulence. the gram-negative nitrogen-fixing soil bacterium rhizobium etli cnpaf512 was previously shown to display swarming behavior on soft agar plates. in a search for novel genetic determinants of swarmi ... | 2008 | 17394038 |
| rhizobium cellulosilyticum sp. nov., isolated from sawdust of populus alba. | during a study of polysaccharide-hydrolysing bacteria present in different plant sources, two strains were isolated from pulverized decaying wood of populus alba and classified in the genus rhizobium on basis of their almost complete 16s rrna gene sequences. their closest phylogenetic relatives were rhizobium galegae usda 4128(t) and rhizobium huautlense s02(t), with 98.2 and 98.1 % 16s rrna gene sequence similarity, respectively. reca and atpd sequence analysis showed that these species have le ... | 2007 | 17392217 |
| the pyda-pydb fusion gene produces an active dioxygenase-hydrolase that degrades 3-hydroxy-4-pyridone, an intermediate of mimosine metabolism. | the objective of this research was to construct a pyda-pydb hybrid gene that encodes a functional dioxygenase-hydrolase (pyda-pydb) fusion protein for degradation of 3-hydroxy-4-pyridone (hp). hp is an intermediate in both synthesis and degradation of mimosine, a toxic amino acid produced by the tree legume leucaena leucocephala. computer-generated models of the fusion proteins suggested that joining of pyda and pydb with 0, 3, or 7 glycine residues as a linker should produce a functional pyda-p ... | 2007 | 17390132 |
| enhancing salt tolerance in a crop plant by overexpression of glyoxalase ii. | earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. we now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase ii. the rice glyoxalase ii was cloned in pcambia1304 and transformed into rice (oryza sativa cv pb1) via agrobacterium. the transgenic plants showed higher constitutive activity of glyoxalase ii that increased further upon salt stress, reflecting the upregulation of endogenous glyoxa ... | 2008 | 17387627 |
| response of inoculated foliar fed pea plants (pisum sativum l.) to reduced mo supply. | the application of nutrients to the roots and leaves of inoculated pea plants grown under conditions of reduced mo supply was studied. pea plants (pisum sativum l.) were grown on liquid nutrient solution excluding mo from the media until the 35th day under glasshouse conditions. plants were inoculated with the bacterial suspension of rhizobium leguminosarum bv. vicae, strain d293 at approximately 10(8) cells per cm3. the foliar fertilizer agroleaf was applied at 0.3% concentration. changes in th ... | 2007 | 17385546 |
| suppressor of rna silencing encoded by the monopartite tomato leaf curl java begomovirus. | we previously isolated the monopartite begomovirus tomato leaf curl java virus (tolcjav) and satellite dnabeta02 from the same naturally infected tomato source in indonesia. tolcjav induced mild leaf curl symptoms in nicotiana benthamiana plants; dnabeta02 encoded the betac1 gene and produced severe leaf curl symptoms when co-inoculated with tolcjav in n. benthamiana. however, dnabeta02mbetac1, which contains a frame shift mutation, did not induce severe symptoms in n. benthamiana when co-inocul ... | 2007 | 17385070 |
| rhizobium tropici response to acidity involves activation of glutathione synthesis. | rhizobium tropici ciat899 displays intrinsic tolerance to acidity, and efficiently nodulates phaseolus vulgaris at low ph. by characterizing a gshb mutant strain, glutathione has been previously demonstrated to be essential for r. tropici tolerance to acid stress. the wild-type gshb gene region has been cloned and its transcription profile has been characterized by using quantitative real-time pcr and transcriptional gene fusions. activation of the gshb gene under acid-stress conditions was demo ... | 2007 | 17379738 |
| rhizobium-initiated rice growth inhibition caused by nitric oxide accumulation. | isolates of rhizobium leguminosarum bv. trifolii (the clover root-nodule endosymbiont) from the nile river delta have been found to infect rice roots and colonize the intercellular spaces of the rice roots. some of these isolates inhibit rice seedling growth but one in particular, r4, has been found in rice roots which develop and grow normally. we present evidence that the induced growth inhibition is due to a toxic accumulation of nitric oxide (no), from the reduction of nitrate, and suggest t ... | 2007 | 17378431 |
| symbiosis-induced cascade regulation of the mesorhizobium loti r7a virb/d4 type iv secretion system. | the mesorhizobium loti r7a symbiosis island contains genes encoding a virb/d4 type iv secretion system (t4ss) similar to that of agrobacterium tumefaciens. this system has host-dependent effects on symbiosis that probably are due to translocation of two effector proteins, msi059 and msi061, into host cells. here we report that, as in a. tumefaciens, the m. loti vir genes are transcriptionally regulated by a vira/virg two-component regulatory system. a virgn54d mutant gene of m. loti caused const ... | 2007 | 17378428 |
| the quorum-sensing transcription factor trar decodes its dna binding site by direct contacts with dna bases and by detection of dna flexibility. | trar of agrobacterium tumefaciens is a member of the luxr family of transcriptional regulators, and binds to specific dna sequences (tra boxes) at target promoters of the tumour-inducing (ti) plasmid. each tra box has a pronounced dyad symmetry, and each subunit of a trar dimer binds to one half of a tra box via a helix-turn-helix (hth) dna binding motif. structural analysis has suggested that trar makes extensive sequence-specific contacts with tra box dna. in this study, we tested these predic ... | 2007 | 17376086 |
| [the pssa gene encodes udp-glucose: polyprenyl phosphate-glucosyl phosphotransferase initiating biosynthesis of rhizobium leguminosarum exopolysaccharide]. | symbiotic nitrogen-fixing bacteria rhizobium leguminosarum by. viciae vf39 secrete an acidic heteropolysaccharide, the biosynthesis of which involves the stage of polyprenyl diphosphate octasaccharide formation, with its carbohydrate fragment corresponding to the repeating polymer unit. the amino acid analysis of the product of the pssa gene, we have earlier identified, showed its homology to bacterial polyisoprenyl phosphate hexose 1-phosphate transferases catalyzing the formation of phosphodie ... | 2007 | 17375671 |
| characterization of t-dna insertion patterns in the genome of rice blast fungus magnaporthe oryzae. | agrobacterium tumefaciens-mediated transformation (atmt) has been proven to be a powerful strategy for gene disruption in plants and fungi. patterns associated with transferred dna (t-dna) integration in plants and yeast have been studied comprehensively, whereas no detailed analysis of t-dna integration has been reported yet in filamentous fungi. here, we reported the t-dna insertion patterns in the genome of filamentous fungus magnaporthe oryzae. using atmt, a t-dna tagged population consistin ... | 2007 | 17372735 |
| hrt and nutrients affect bacterial communities grown on recirculation aquaculture system effluents. | in a recirculation aquaculture system the drumfilter effluent can be used as substrate for heterotrophic bacterial production, which can be recycled as feed. because the bacteria might contain pathogens, which could reduce its suitability as feed, it is important to characterize these communities. bacteria were produced in growth reactors under different conditions: 7 h hydraulic retention time (hrt) vs. 2 h, sodium acetate vs. molasses, and ammonia vs. nitrate. the community of the drumfilter e ... | 2007 | 17371322 |
| caspase-resistant vird2 protein provides enhanced gene delivery and expression in plants. | agrobacterium tumefaciens vird2 protein is one of the key elements of agrobacterium-mediated plant transformation, a process of transfer of t-dna sequence from the agrobacterium tumour inducing plasmid into the nucleus of infected plant cells and its integration into the host genome. the vird2 protein has been shown to be a substrate for a plant caspase-like protease activity (pclp) in tobacco. we demonstrate here that mutagenesis of the vird2 protein to prevent cleavage by pclp increases the ef ... | 2007 | 17370074 |
| traa, trac and trad autorepress two divergent quorum-regulated promoters near the transfer origin of the ti plasmid of agrobacterium tumefaciens. | whole-genome transcriptional profiling experiments were performed to identify the complete set of trar-regulated genes in isogenic a. tumefaciens strains containing an octopine-type or nopaline-type ti plasmid. most of the known trar-regulated genes as well as a number of new inducible genes were identified. surprisingly, some known members of this regulon showed both weaker induction and weak levels of expression than we had predicted based upon earlier studies. in particular, traa was expresse ... | 2007 | 17367394 |
| [obtaining marker-free transgenic soybean plants with optimal frequency by constructing three t-dnas binary vector]. | obtaining marker-free plants with high efficiency will benefit the environmental release of transgenic crops. to achieve this point, a binary vector pnb35svip1 with three t-dnas was constructed by using several mediate plasmids, in which one copy of bar gene expression cassette and two copies of vip1 gene expression cassette were included. eha101 agrobacterium strain harboring the final construct was applied to transform soybean (glycine max) cotyledon nodes. through 2 - 3 months regeneration an ... | 2007 | 17366903 |
| cell-cell communication in the plant pathogen agrobacterium tumefaciens. | the plant pathogen agrobacterium tumefaciens induces the formation of crown gall tumours at wound sites on host plants by directly transforming plant cells. this disease strategy benefits the bacteria as the infected plant tissue produces novel nutrients, called opines, that the colonizing bacteria can use as nutrients. almost all of the genes that are required for virulence, and all of the opine uptake and utilization genes, are carried on large tumour-inducing (ti) plasmids. the observation mo ... | 2007 | 17360279 |
| quorum-sensing regulation in rhizobia and its role in symbiotic interactions with legumes. | legume-nodulating bacteria (rhizobia) usually produce n-acyl homoserine lactones, which regulate the induction of gene expression in a quorum-sensing (or population-density)-dependent manner. there is significant diversity in the types of quorum-sensing regulatory systems that are present in different rhizobia and no two independent isolates worked on in detail have the same complement of quorum-sensing genes. the genes regulated by quorum sensing appear to be rather diverse and many are associa ... | 2007 | 17360278 |
| molecular diversity of legume root-nodule bacteria in kakadu national park, northern territory, australia. | symbiotic relationships between leguminous plants (family fabaceae) and nodule-forming bacteria in australia native ecosystems remain poorly characterized despite their importance. most studies have focused on temperate parts of the country, where the use of molecular approaches have already revealed the presence of bradyrhizobium, ensifer (formerly sinorhizobium), mesorhizobium and rhizobium genera of legume root-nodule bacteria. we here provide the first molecular characterization of nodulatin ... | 2007 | 17356689 |
| genome-wide functional analysis of pathogenicity genes in the rice blast fungus. | rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, magnaporthe oryzae. in total, we produced 21,070 mutants through l ... | 2007 | 17353894 |
| characterization and transient replication of tomato leaf curl virus defective dnas. | distinct subgenomic dna species known as defective (df) dna molecules were found in plants infected with tomato leaf curl virus (tlcv). four df dnas derived from tlcv type and darwin 1 strains were found to contain large deletions that disrupt all of the viral genes required for viral replication, encapsidation and spread. however, the viral origin of replication (ori), including the replication-associated protein (rep) binding domains, was present in all four df dnas. co-agroinfection of leaf s ... | 2007 | 17347773 |
| nuclear labeling of coccidioides posadasii with green fluorescent protein. | coccidioidomycosis is a mild to life-threatening disease in otherwise healthy humans and other mammals caused by the fungus coccidioides spp. understanding the development of the unique dimorphic life cycle of coccidioides spp. and its role in pathogenesis has been an area of research focus. however, nuclear behavior during the saprobic and parasitic life cycle has not been studied intensively. in this study, green fluorescent protein (gfp) was fused to histone h1 and introduced into coccidioide ... | 2007 | 17344520 |
| silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers. | beta-carotene is the main dietary precursor of vitamin a. potato tubers contain low levels of carotenoids, composed mainly of the xanthophylls lutein (in the beta-epsilon branch) and violaxanthin (in the beta-beta branch). none of these carotenoids have provitamin a activity. we have previously shown that tuber-specific silencing of the first step in the epsilon-beta branch, lcy-e, redirects metabolic flux towards beta-beta carotenoids, increases total carotenoids up to 2.5-fold and beta-caroten ... | 2007 | 17335571 |
| metal induction of a paxillus involutus metallothionein and its heterologous expression in hebeloma cylindrosporum. | * metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. here we characterized the pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus paxillus involutus. * expression of pimt1 in p. involutus under metal stress conditions was measured by northern blot and rt-pcr analyses. the full-length cdna was used to perform functional complementation in yeast mutant strains and agrotransformation of hebeloma cylindrosporum. * heterologous expression in ... | 2007 | 17335505 |
| evolving ideas of legume evolution and diversity: a taxonomic perspective on the occurrence of nodulation. | legumes evolved about 60 million years ago (ma), and nodulation 58 ma. nonnodulation remains common in caesalpinioideae, with smaller numbers in mimosoideae and papilionoideae. the first type of infection by bacteria may have been at junctions where lateral roots emerged, followed by formation of infection threads to confine bacteria and convey them to some cells in the developing nodule, where they were generally released into symbiosomes. infection threads were a prerequisite for root-hair inf ... | 2007 | 17335493 |
| inhibition of lipid a biosynthesis as the primary mechanism of chir-090 antibiotic activity in escherichia coli. | the deacetylation of udp-3-o-[(r)-3-hydroxymyristoyl]-n-acetylglucosamine (udp-3-o-acyl-glcnac) by lpxc is the committed reaction of lipid a biosynthesis. chir-090, a novel n-aroyl-l-threonine hydroxamic acid, is a potent, slow, tight-binding inhibitor of the lpxc deacetylase from the hyperthermophile aquifex aeolicus, and it has excellent antibiotic activity against pseudomonas aeruginosa and escherichia coli, as judged by disk diffusion assays. we now report that chir-090 is also a two-step sl ... | 2007 | 17335290 |
| protein conformational changes of agrobacterium phytochrome agp1 during chromophore assembly and photoconversion. | phytochromes are widely distributed photochromic biliprotein photoreceptors. typical bacterial phytochromes such as agrobacterium agp1 have a c-terminal histidine kinase module; the n-terminal chromophore module induces conformational changes in the protein that lead to modulation of kinase activity. we show by protein cross-linking that the c-terminal histidine kinase module of agp1 mediates stable dimerization. the fragment agp1-m15, which comprises the chromophore module but lacks the histidi ... | 2007 | 17335289 |
| making a friend from a foe: expressing a groel gene from the whitefly bemisia tabaci in the phloem of tomato plants confers resistance to tomato yellow leaf curl virus. | some (perhaps all) plant viruses transmitted in a circulative manner by their insect vectors avoid destruction in the haemolymph by interacting with groel homologues, ensuring transmission. we have previously shown that the phloem-limited begomovirus tomato yellow leaf curl virus (tylcv) interacts in vivo and in vitro with groel produced by the whitefly vector bemisia tabaci. in this study, we have exploited this phenomenon to generate transgenic tomato plants expressing the whitefly groel in th ... | 2007 | 17334947 |
| verification and rapid identification of soybean rhizobia in indian soils. | sixty root nodule isolates of soybean rhizobia indigenous to eight field sites in india were characterized using pcr-rflp for repeated sequence rsalpha a 1195-bp dna fragment, indole acetic acid production, and nitrogenase activity. site-dependent variations were observed in terms of iaa production and nitrogenase activities. rsalpha was conserved in slow-growing soybean rhizobia across locations and sites and was absent in other rhizobiaceae members and other bacterial genera. the results sugge ... | 2007 | 17334844 |
| [t-dna-induced mutations in transgenic plants]. | the review surveys experimental data on changes of individual traits in genetically modified (transgenic) plants. the attention is focused on mutations induced by t-dna insertions upon agrobacterium-induced transformation of dicotyledonous plants. the character of mutation appearance in transgenic plants is examined. the prospects of mutations induced by t-dna insertions are considered. | 2007 | 17333933 |
| microbial enzymes involved in lactone compound metabolism and their biotechnological applications. | lactone compounds are widely distributed in nature and play important roles in organisms. these compounds are synthesized and metabolized enzymatically in vivo; however, detailed investigation of these enzymes lags behind that of other common enzymes. in this paper, recent work on the enzymes involved in the metabolism of lactone compounds will be reviewed. in particular, fundamental and application studies on lactonases and baeyer-villiger monooxgenases of microbial origin are described. | 2007 | 17333168 |
| salidroside production by hairy roots of rhodiola sachalinensis obtained after transformation with agrobacterium rhizogenes. | hairy roots induced by agrobacterium rhizogenes grow faster, and are considered as genetically stable. these hairy roots can be used as an interesting material for the production of secondary metabolites of pharmaceutical value. salidroside has been identified as the major compounds from the roots of rhodiola sachalinensis a. bor. here, we provide an update that adds new perspectives on the prospects and challenges of producing salidroside from hairy roots induced by agrobacterium rhizogene in r ... | 2007 | 17329834 |
| chemolithoautotrophic oxidation of thiosulfate, tetrathionate and thiocyanate by a novel rhizobacterium belonging to the genus paracoccus. | two tropical leguminous-rhizospheric strains, sst and jt 001, phylogenetically closest to paracoccus thiocyanatus and paracoccus pantotrophus, respectively, were isolated on reduced sulfur compounds as sole energy and electron sources. while sst had versatile chemolithotrophic abilities to oxidize thiosulfate, tetrathionate, thiocyanate, sulfide and elemental sulfur, jt 001 could oxidize thiosulfate, soluble sulfide, elemental sulfur and a relatively lesser amount of tetrathionate. positive hybr ... | 2007 | 17326754 |
| characterization of vascular-specific rss1 and rolc promoters for their utilization in engineering plants to develop resistance against hemipteran insect pests. | rice sucrose synthase1, rss1 (isolated from rice) and rolc (isolated from agrobacterium rhizogenes) promoters were evaluated by binding analyses of their respective cis-elements with host nuclear transcription factors. the expression profile of an insecticidal protein driven by these promoters in transgenic plants was monitored. motif-search analysis with available phloem-specific promoter sequences revealed the presence of two boxii elements in rss1. an octopine synthase element, a stem-specifi ... | 2007 | 17323077 |
| crystal structure of agr_c_4470p from agrobacterium tumefaciens. | we report here the crystal structure at 2.0 a resolution of the agr_c_4470p protein from the gram-negative bacterium agrobacterium tumefaciens. the protein is a tightly associated dimer, each subunit of which bears strong structural homology with the two domains of the heme utilization protein chus from escherichia coli and hems from yersinia enterocolitica. remarkably, the organization of the agr_c_4470p dimer is the same as that of the two domains in chus and hems, providing structural evidenc ... | 2007 | 17322535 |
| gene targeting in gram-negative bacteria by use of a mobile group ii intron ("targetron") expressed from a broad-host-range vector. | mobile group ii introns ("targetrons") can be programmed for insertion into virtually any desired dna target with high frequency and specificity. here, we show that targetrons expressed via an m-toluic acid-inducible promoter from a broad-host-range vector containing an rk2 minireplicon can be used for efficient gene targeting in a variety of gram-negative bacteria, including escherichia coli, pseudomonas aeruginosa, and agrobacterium tumefaciens. targetrons expressed from donor plasmids introdu ... | 2007 | 17322321 |
| changes in motility of the rhizobacterium azospirillum brasilense in the presence of plant lectins. | the plant-beneficial bacterium azospirillum brasilense can swim in liquids and swarm or migrate with the formation of microcolonies in soft media. to get closer to understanding the influence of natural environments on a. brasilense motility, we studied the individual and social movement of the bacterium in the presence of various plant lectins. the lectins with specificity for n-acetyl-beta-d-glucosamine oligomers (wheat germ, solanum tuberosum and ulex europeus agglutinins) decreased a. brasil ... | 2009 | 17317126 |
| bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an auxin- and ethylene-independent signaling mechanism in arabidopsis thaliana. | soil microorganisms are critical players in plant-soil interactions at the rhizosphere. we have identified a bacillus megaterium strain that promoted growth and development of bean (phaseolus vulgaris) and arabidopsis thaliana plants. we used arabidopsis thaliana as a model to characterize the effects of inoculation with b. megaterium on plant-growth promotion and postembryonic root development. b. megaterium inoculation caused an inhibition in primary-root growth followed by an increase in late ... | 2007 | 17313171 |
| identification and characterization of a nodh ortholog from the alfalfa-nodulating or191-like rhizobia. | nodulation of medicago sativa (alfalfa) is known to be restricted to sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to rhizobium sp. strain or191. distinctive features of the symbiosis between alfalfa and s. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (nod factors [nfs]) at its reducing end. here, we present evidence of the presence of a ... | 2007 | 17313165 |
| living without fur: the subtlety and complexity of iron-responsive gene regulation in the symbiotic bacterium rhizobium and other alpha-proteobacteria. | the alpha-proteobacteria include several important genera, including the symbiotic n(2)-fixing "rhizobia", the plant pathogen agrobacterium, the mammalian pathogens brucella, bartonella as well as many others that are of environmental or other interest--including rhodobacter, caulobacter and the hugely abundant marine genus pelagibacter. only a few species--mainly different members of the rhizobia--have been analyzed directly for their ability to use and to respond to iron. these studies, howeve ... | 2007 | 17310401 |
| transient beta-glucuronidase expression in lily (lilium longflorum l.) pollen via wounding-assisted agrobacterium-mediated transformation. | a pollen-based transient expression system has been developed. lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with agrobacterium tumefaciens lba4404 cells harboring the beta-glucuronidase (gus) gene construct, pbi121. in histochemical and fluorometric gus analysis, the wounding processes allowed efficient transformation and, in cdna blot hybridization, gus mrna synthesis was clearly detected. lily pollen with appropri ... | 2007 | 17310322 |
| production of transgenic strawberries by temporary immersion bioreactor system and verification by tail-pcr. | strawberry (fragaria x ananassa) is an economically important soft fruit crop with polyploid genome which complicates the breeding of new cultivars. for certain traits, genetic engineering offers a potential alternative to traditional breeding. however, many strawberry varieties are quite recalcitrant for agrobacterium-mediated transformation, and a method allowing easy handling of large amounts of starting material is needed. also the genotyping of putative transformants is challenging since th ... | 2007 | 17309794 |
| agrobacterium-mediated transformation of cereals: a promising approach crossing barriers. | cereal crops have been the primary targets for improvement by genetic transformation because of their worldwide importance for human consumption. for a long time, many of these important cereals were difficult to genetically engineer, mainly as a result of their inherent limitations associated with the resistance to agrobacterium infection and their recalcitrance to in vitro regeneration. the delivery of foreign genes to rice plants via agrobacterium tumefaciens has now become a routine techniqu ... | 2006 | 17309731 |
| a chemically inducible cucumber mosaic virus amplicon system for expression of heterologous proteins in plant tissues. | a novel cucumber mosaic virus inducible viral amplicon (cmviva) expression system has been developed that allows for tightly regulated chemically inducible expression of heterologous genes in plant hosts. transient production of recombinant alpha(1)-antitrypsin (raat), a human blood protein, was demonstrated in nicotiana benthamiana leaves. the highest production levels were obtained by co-infiltrating leaves with agrobacterium tumefaciens cells containing cmviva carrying the aat gene and a. tum ... | 2006 | 17309729 |
| coupling two mercury resistance genes in eastern cottonwood enhances the processing of organomercury. | eastern cottonwood (populus deltoides bartr. ex marsh.) trees were engineered to express mera (mercuric ion reductase) and merb (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. earlier studies with arabidopsis thaliana and nicotiana tabacum showed that this gene combination resulted in more efficient detoxification of organomercurial compounds than did merb alone, but neither species is optimal for long-term field applications. leaf dis ... | 2007 | 17309680 |
| genetic diversity of rhizobia associated with desmodium species grown in china. | desmodia are leguminous plants used as important forage and herbal medicine in china. little information is available about the nodule bacteria of desmodium species. to understand the genetic diversity of rhizobia associated with desmodium species grown in china, isolates from temperate and subtropical regions were obtained and analysed. | 2007 | 17309506 |
| development of a simple and rapid agrobacterium tumefaciens-mediated transformation system for the entomopathogenic fungus metarhizium anisopliae var. acridum. | to examine the ability of agrobacterium to attach to metarhizium anisopliae var. acridum strain cg423 under co-cultivation and to develop an agrobacterium-mediated method of gene delivery into strain cg423, a promising agent for biological control of grasshoppers. | 2007 | 17309500 |